Rroxscaffold_7G00159060

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010000
Physical Location & Seq
Reverse (-)
2356056 .. 2358863
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159060.1

Sequence Viewer

Length: 1233 bp
ATGTCATGGCTCGCCCACTCGCTCTCTAAATCCCTCCGAATCGACGGCGACGACGGCGACGGCGAAGGCCAAGATCACGACGACGACGTCGTACCCCACCACCACACCAACAATGAAGCTGACGTCCCGCCCACCGCCTCCACCAAACCCCAAGACAACGAAATCCAATCGGATGCAGAAGCAGTTGAAGACGCCCAAACGCGTGGCGTGAAAGAAGACCTATCGGAATTCCAGCAAACCCTAACGCGCCAATTCTGGGGCGTCGCCTCGTTCCTTGCGCCACCACCGCCCTCTGATCGATTAGCTTCTCCGCCCGATTGCGATCGATCCGAGCCGGCCGATCACCCACCCGGTCAATCGCCGATGGCGTTCAATGAAGACGAAGAGGAGGAGGAGGAGTGCGCCGTCGGTGTCACTGACGAGGTCTTGGCCTTCGCTAGAAACATCGCGATGCATCCAGAGACCTGGTTGGACTTTCCTCTTGATGAAGAAGAAGACTTGGGAGATTTTGAAATGTCTGATGCCCAGCAAGAGCATGCTATGATGATTGAACATCTTGCGCCGAGATTAGCTGCTTTGAGAATTGAGCTTTGTCCGTGCCATATGAGCGAGAGTTACTTCTGGAAAGTCTATTTTGTTCTTCTGCATTCGAGGCTCAACAAGCAGGATGCTGAGGTTTTGTCCACACCTCAGGTAATGGAAGCCAGAGCAATGTGGATGCAAGAGCTACAATGTCAGACTCCATCATCAATTTCTAAAATGTCAGAACATGAATGGTTTGGAAGAAGCACTTCACATTTAAAATACAGTTCAAATGCAATACATGAAGATTATGAAGATTACTCTCCGGTCTCATCACATACTCCTCATTATGAGGATACACTTCATAAGACGTATCCTTTTGAATCAACCATGCCTTCCACAGCCGACGAGACTGAGAAGCACCCAGTTGAGAGTAATGAGATGCCATTCATTGATAAGTCAGTTATTGAAGAAAAGCCCTTCATTAGGACTGAGGATAAGAATGTGACGGTTGGTCCATCCTCAGACATACTAGTTAATAGCTATGACGACGACGACGACGATGATGACTGGCCAGAGGATGATGGTGAATTATATGGGTGTAGTGGAACAGCTGTTGCTTTGATAAATGAAGAAGACATATCTTTCAGTGATCTTGAGGATGATGATTTCCAGGCACCCTCTAAATTAAAGTTCATCTCAAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

410

Amino Acids

46.35

Weight (kDa)

4.22

Isoelectric Point (pI)

66.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 168 - 223 1.7e-13 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AatII GACGTC 2 cut(s) 90, 126
AccB1I GGYRCC 1 cut(s) 1198
AccII CGCG 3 cut(s) 202, 247, 449
AciI CCGC 4 cut(s) 128, 135, 287, 311
AclWI GGATC 1 cut(s) 321
AcoI YGGCCR 2 cut(s) 336, 1094
AcsI RAATTY 1 cut(s) 227
AcyI GRCGYC 4 cut(s) 87, 123, 192, 261
AfaI GTAC 1 cut(s) 93
AfiI CCNNNNNNNGG 2 cut(s) 256, 1008
AflIII ACRYGT 1 cut(s) 200
AgsI TTSAA 7 cut(s) 188, 373, 512, 551, 813, 905, 992
AhdI GACNNNNNGTC 1 cut(s) 1035
AhlI ACTAGT 1 cut(s) 1054
AjnI CCWGG 2 cut(s) 464, 1194
AluBI AGCT 7 cut(s) 119, 305, 572, 589, 727, 1065, 1136
AluI AGCT 7 cut(s) 119, 305, 572, 589, 727, 1065, 1136
Alw26I GTCTC 3 cut(s) 455, 856, 926
AlwI GGATC 1 cut(s) 321
AoxI GGCC 4 cut(s) 67, 336, 429, 1094
ApeKI GCWGC 1 cut(s) 572
ApoI RAATTY 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 790
AspLEI GCGC 4 cut(s) 249, 280, 404, 562
AspS9I GGNCC 1 cut(s) 1037
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 2 cut(s) 335, 1121
AvaII GGWCC 1 cut(s) 1037
AxyI CCTNAGG 1 cut(s) 690
BalI TGGCCA 1 cut(s) 1096
BanI GGYRCC 1 cut(s) 1198
BbsI GAAGAC 5 cut(s) 195, 222, 384, 501, 1164
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 1 cut(s) 559
BccI CCATC 4 cut(s) 358, 751, 1048, 1100
BceAI ACGGC 4 cut(s) 61, 70, 76, 389
BciT130I CCWGG 2 cut(s) 466, 1196
BciVI GTATCC 2 cut(s) 871, 906
BcnI CCSGG 1 cut(s) 351
BcoDI GTCTC 3 cut(s) 455, 856, 926
BcuI ACTAGT 1 cut(s) 1054
BfaI CTAG 2 cut(s) 438, 1055
BfuI GTATCC 2 cut(s) 871, 906
BisI GCNGC 1 cut(s) 573
BlsI GCNGC 1 cut(s) 574
Bme1390I CCNGG 3 cut(s) 351, 466, 1196
Bme18I GGWCC 1 cut(s) 1037
BmeRI GACNNNNNGTC 1 cut(s) 1035
BmgT120I GGNCC 1 cut(s) 1037
BmiI GGNNCC 1 cut(s) 1200
BmrFI CCNGG 3 cut(s) 351, 466, 1196
BmrI ACTGGG 1 cut(s) 941
BmsI GCATC 7 cut(s) 163, 441, 463, 511, 658, 708, 954
BmuI ACTGGG 1 cut(s) 941
BpiI GAAGAC 5 cut(s) 195, 222, 384, 501, 1164
Bpu10I CCTNAGC 1 cut(s) 672
BpuEI CTTGAG 1 cut(s) 1199
BpuMI CCSGG 1 cut(s) 351
Bsa29I ATCGAT 2 cut(s) 298, 325
BsaBI GATNNNNATC 1 cut(s) 321
BsaHI GRCGYC 4 cut(s) 87, 123, 192, 261
BsaI GGTCTC 2 cut(s) 455, 856
BsaWI WCCGGW 1 cut(s) 847
BsaXI ACNNNNNCTCC 2 cut(s) 383, 413
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 1008
Bse118I RCCGGY 1 cut(s) 334
Bse1I ACTGG 2 cut(s) 947, 1097
Bse21I CCTNAGG 1 cut(s) 690
Bse3DI GCAATG 1 cut(s) 717
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 2 cut(s) 466, 1196
BseCI ATCGAT 2 cut(s) 298, 325
BseGI GGATG 7 cut(s) 178, 454, 673, 723, 1040, 1108, 1189
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 256, 1008
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 5 cut(s) 663, 704, 927, 1005, 1059
BseNI ACTGG 2 cut(s) 947, 1097
BseRI GAGGAG 5 cut(s) 401, 404, 407, 410, 855
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 1 cut(s) 559
BseYI CCCAGC 1 cut(s) 525
Bsh1236I CGCG 3 cut(s) 202, 247, 449
Bsh1285I CGRYCG 2 cut(s) 325, 339
BshFI GGCC 4 cut(s) 69, 338, 431, 1096
BshNI GGYRCC 1 cut(s) 1198
BshVI ATCGAT 2 cut(s) 298, 325
BsiEI CGRYCG 2 cut(s) 325, 339
BsiSI CCGG 3 cut(s) 335, 351, 848
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 256, 1008
BsmAI GTCTC 3 cut(s) 455, 856, 926
BsmFI GGGAC 1 cut(s) 110
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 4 cut(s) 69, 338, 431, 1096
Bso31I GGTCTC 2 cut(s) 455, 856
Bsp143I GATC 6 cut(s) 73, 295, 322, 326, 340, 1174
Bsp68I TCGCGA 1 cut(s) 449
BspACI CCGC 4 cut(s) 128, 135, 287, 311
BspANI GGCC 4 cut(s) 69, 338, 431, 1096
BspCNI CTCAG 5 cut(s) 664, 703, 928, 1006, 1058
BspDI ATCGAT 2 cut(s) 298, 325
BspFNI CGCG 3 cut(s) 202, 247, 449
BspLI GGNNCC 1 cut(s) 1200
BspPI GGATC 1 cut(s) 321
BspT107I GGYRCC 1 cut(s) 1198
BspTNI GGTCTC 2 cut(s) 455, 856
BsrDI GCAATG 1 cut(s) 717
BsrFI RCCGGY 1 cut(s) 334
BsrI ACTGG 2 cut(s) 947, 1097
BssAI RCCGGY 1 cut(s) 334
BssMI GATC 6 cut(s) 73, 295, 322, 326, 340, 1174
BssNI GRCGYC 4 cut(s) 87, 123, 192, 261
Bst2UI CCWGG 2 cut(s) 466, 1196
Bst4CI ACNGT 2 cut(s) 809, 1033
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 4 cut(s) 87, 123, 192, 261
BstC8I GCNNGC 3 cut(s) 12, 336, 537
BstDEI CTNAG 5 cut(s) 672, 690, 936, 1014, 1045
BstENI CCTNNNNNAGG 1 cut(s) 1006
BstF5I GGATG 7 cut(s) 178, 454, 673, 723, 1040, 1108, 1189
BstFNI CGCG 3 cut(s) 202, 247, 449
BstHHI GCGC 4 cut(s) 249, 280, 404, 562
BstKTI GATC 6 cut(s) 76, 298, 325, 329, 343, 1177
BstMAI GTCTC 3 cut(s) 455, 856, 926
BstMBI GATC 6 cut(s) 73, 295, 322, 326, 340, 1174
BstMCI CGRYCG 2 cut(s) 325, 339
BstMWI GCNNNNNNNGC 5 cut(s) 54, 286, 606, 652, 661
BstNI CCWGG 2 cut(s) 466, 1196
BstNSI RCATGY 1 cut(s) 539
BstSCI CCNGG 3 cut(s) 349, 464, 1194
BstUI CGCG 3 cut(s) 202, 247, 449
BstV1I GCAGC 1 cut(s) 559
BstV2I GAAGAC 5 cut(s) 195, 222, 384, 501, 1164
BstXI CCANNNNNNTGG 2 cut(s) 203, 465
BstZI CGGCCG 1 cut(s) 336
Bsu15I ATCGAT 2 cut(s) 298, 325
Bsu36I CCTNAGG 1 cut(s) 690
BsuI GTATCC 2 cut(s) 871, 906
BsuRI GGCC 4 cut(s) 69, 338, 431, 1096
BsuTUI ATCGAT 2 cut(s) 298, 325
BtgZI GCGATG 2 cut(s) 430, 464
BtsCI GGATG 7 cut(s) 178, 454, 673, 723, 1040, 1108, 1189
BtsIMutI CAGTG 2 cut(s) 414, 1177
BtuMI TCGCGA 1 cut(s) 449
Cac8I GCNNGC 3 cut(s) 12, 336, 537
CfoI GCGC 4 cut(s) 249, 280, 404, 562
Cfr10I RCCGGY 1 cut(s) 334
Cfr13I GGNCC 1 cut(s) 1037
ClaI ATCGAT 2 cut(s) 298, 325
CseI GACGC 2 cut(s) 200, 250
CsiI ACCWGGT 1 cut(s) 464
Csp6I GTAC 1 cut(s) 92
CviAII CATG 5 cut(s) 6, 536, 770, 824, 913
CviQI GTAC 1 cut(s) 92
DdeI CTNAG 5 cut(s) 672, 690, 936, 1014, 1045
DpnI GATC 6 cut(s) 75, 297, 324, 328, 342, 1176
DpnII GATC 6 cut(s) 73, 295, 322, 326, 340, 1174
DraI TTTAAA 1 cut(s) 801
DrdI GACNNNNNNGTC 1 cut(s) 86
DriI GACNNNNNGTC 1 cut(s) 1035
DseDI GACNNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 2 cut(s) 336, 1094
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 378
Eam1105I GACNNNNNGTC 1 cut(s) 1035
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 1 cut(s) 300
EclXI CGGCCG 1 cut(s) 336
Eco31I GGTCTC 2 cut(s) 455, 856
Eco47I GGWCC 1 cut(s) 1037
Eco52I CGGCCG 1 cut(s) 336
Eco81I CCTNAGG 1 cut(s) 690
EcoNI CCTNNNNNAGG 1 cut(s) 1006
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 464, 1194
EcoT22I ATGCAT 1 cut(s) 456
FaeI CATG 5 cut(s) 9, 539, 773, 827, 916
FalI AAGNNNNNCTT 2 cut(s) 775, 807
FaqI GGGAC 1 cut(s) 110
FatI CATG 5 cut(s) 5, 535, 769, 823, 912
FauI CCCGC 1 cut(s) 135
FauNDI CATATG 1 cut(s) 603
Fnu4HI GCNGC 1 cut(s) 573
FokI GGATG 7 cut(s) 185, 441, 680, 730, 1027, 1115, 1196
Fsp4HI GCNGC 1 cut(s) 573
FspBI CTAG 2 cut(s) 438, 1055
GlaI GCGC 4 cut(s) 248, 279, 403, 561
GluI GCNGC 1 cut(s) 573
GsaI CCCAGC 1 cut(s) 529
HaeIII GGCC 4 cut(s) 69, 338, 431, 1096
HapII CCGG 3 cut(s) 335, 351, 848
HgaI GACGC 2 cut(s) 200, 250
HhaI GCGC 4 cut(s) 249, 280, 404, 562
Hin1I GRCGYC 4 cut(s) 87, 123, 192, 261
Hin1II CATG 5 cut(s) 9, 539, 773, 827, 916
Hin6I GCGC 4 cut(s) 247, 278, 402, 560
HinP1I GCGC 4 cut(s) 247, 278, 402, 560
HinfI GANTC 3 cut(s) 39, 739, 905
HpaII CCGG 3 cut(s) 335, 351, 848
HphI GGTGA 2 cut(s) 335, 1121
Hpy166II GTNNAC 1 cut(s) 684
Hpy188I TCNGA 9 cut(s) 38, 172, 226, 295, 331, 520, 738, 766, 1048
Hpy188III TCNNGA 6 cut(s) 77, 448, 458, 482, 622, 1178
Hpy8I GTNNAC 1 cut(s) 684
HpyAV CCTTC 4 cut(s) 59, 442, 927, 1012
HpyCH4III ACNGT 2 cut(s) 809, 1033
HpyCH4IV ACGT 3 cut(s) 87, 123, 893
HpyCH4V TGCA 5 cut(s) 176, 454, 646, 721, 818
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 286, 606, 652, 661
HpyF3I CTNAG 5 cut(s) 672, 690, 936, 1014, 1045
HpySE526I ACGT 3 cut(s) 87, 123, 893
Hsp92I GRCGYC 4 cut(s) 87, 123, 192, 261
Hsp92II CATG 5 cut(s) 9, 539, 773, 827, 916
HspAI GCGC 4 cut(s) 247, 278, 402, 560
KroI GCCGGC 1 cut(s) 334
KroNI GCCGGC 1 cut(s) 336
Kzo9I GATC 6 cut(s) 73, 295, 322, 326, 340, 1174
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 7 cut(s) 163, 441, 463, 511, 658, 708, 954
MabI ACCWGGT 1 cut(s) 464
MaeI CTAG 2 cut(s) 438, 1055
MaeII ACGT 3 cut(s) 87, 123, 893
MaeIII GTNAC 3 cut(s) 412, 614, 1027
MalI GATC 6 cut(s) 75, 297, 324, 328, 342, 1176
MboI GATC 6 cut(s) 73, 295, 322, 326, 340, 1174
MlsI TGGCCA 1 cut(s) 1096
MluCI AATT 6 cut(s) 227, 251, 582, 750, 1112, 1208
MluI ACGCGT 1 cut(s) 200
MluNI TGGCCA 1 cut(s) 1096
MlyI GAGTC 1 cut(s) 733
MmeI TCCRAC 1 cut(s) 450
Mox20I TGGCCA 1 cut(s) 1096
Mph1103I ATGCAT 1 cut(s) 456
MroNI GCCGGC 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 790
MscI TGGCCA 1 cut(s) 1096
MseI TTAA 3 cut(s) 800, 1059, 1211
MslI CAYNNNNRTG 1 cut(s) 449
Msp20I TGGCCA 1 cut(s) 1096
MspA1I CMGCKG 1 cut(s) 1136
MspI CCGG 3 cut(s) 335, 351, 848
MspR9I CCNGG 3 cut(s) 351, 466, 1196
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 2 cut(s) 466, 1196
MvnI CGCG 3 cut(s) 202, 247, 449
MwoI GCNNNNNNNGC 5 cut(s) 54, 286, 606, 652, 661
NaeI GCCGGC 1 cut(s) 336
NciI CCSGG 1 cut(s) 351
NdeI CATATG 1 cut(s) 603
NdeII GATC 6 cut(s) 73, 295, 322, 326, 340, 1174
NgoMIV GCCGGC 1 cut(s) 334
NlaIII CATG 5 cut(s) 9, 539, 773, 827, 916
NlaIV GGNNCC 1 cut(s) 1200
NmeAIII GCCGAG 1 cut(s) 588
NmuCI GTSAC 2 cut(s) 412, 1027
NruI TCGCGA 1 cut(s) 449
NsiI ATGCAT 1 cut(s) 456
NspI RCATGY 1 cut(s) 539
PaeI GCATGC 1 cut(s) 539
PcsI WCGNNNNNNNCGW 7 cut(s) 48, 60, 84, 87, 365, 1077, 1080
PctI GAATGC 1 cut(s) 646
PdiI GCCGGC 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 790
PfeI GAWTC 2 cut(s) 39, 905
PflFI GACNNNGTC 2 cut(s) 86, 422
PkrI GCNGC 1 cut(s) 574
Ple19I CGATCG 1 cut(s) 325
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
Psp6I CCWGG 2 cut(s) 464, 1194
PspFI CCCAGC 1 cut(s) 525
PspGI CCWGG 2 cut(s) 464, 1194
PspN4I GGNNCC 1 cut(s) 1200
PspPI GGNCC 1 cut(s) 1037
PsyI GACNNNGTC 2 cut(s) 86, 422
PvuI CGATCG 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 1136
RruI TCGCGA 1 cut(s) 449
RsaI GTAC 1 cut(s) 93
RsaNI GTAC 1 cut(s) 92
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 3 cut(s) 800, 1059, 1211
SatI GCNGC 1 cut(s) 573
Sau3AI GATC 6 cut(s) 73, 295, 322, 326, 340, 1174
Sau96I GGNCC 1 cut(s) 1037
SchI GAGTC 1 cut(s) 733
ScrFI CCNGG 3 cut(s) 351, 466, 1196
SexAI ACCWGGT 1 cut(s) 464
SfaNI GCATC 7 cut(s) 163, 441, 463, 511, 658, 708, 954
SinI GGWCC 1 cut(s) 1037
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 1178
SmoI CTYRAG 1 cut(s) 1178
SpeI ACTAGT 1 cut(s) 1054
SphI GCATGC 1 cut(s) 539
Sse9I AATT 6 cut(s) 227, 251, 582, 750, 1112, 1208
SsiI CCGC 4 cut(s) 128, 135, 287, 311
SspMI CTAG 2 cut(s) 438, 1055
StyD4I CCNGG 3 cut(s) 349, 464, 1194
TaaI ACNGT 2 cut(s) 809, 1033
TaiI ACGT 3 cut(s) 90, 126, 896
TaqI TCGA 4 cut(s) 42, 298, 325, 650
TasI AATT 6 cut(s) 227, 251, 582, 750, 1112, 1208
TfiI GAWTC 2 cut(s) 39, 905
Tru1I TTAA 3 cut(s) 800, 1059, 1211
Tru9I TTAA 3 cut(s) 800, 1059, 1211
TscAI CASTG 2 cut(s) 421, 1177
TseFI GTSAC 2 cut(s) 412, 1027
TseI GCWGC 1 cut(s) 572
Tsp45I GTSAC 2 cut(s) 412, 1027
TspGWI ACGGA 1 cut(s) 585
TspRI CASTG 2 cut(s) 421, 1177
Tth111I GACNNNGTC 2 cut(s) 86, 422
VpaK11BI GGWCC 1 cut(s) 1037
XagI CCTNNNNNAGG 1 cut(s) 1006
XapI RAATTY 1 cut(s) 227
XceI RCATGY 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 790
XspI CTAG 2 cut(s) 438, 1055
ZraI GACGTC 2 cut(s) 88, 124
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.