MD15G1066600.v1.1

BSD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010000
Physical Location & Seq
Reverse (-)
4632386 .. 4635023
2638 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1066600.v1.1.491

Sequence Viewer

Length: 1206 bp
ATGTCATGGCTCGCTCGCTCCCTCGCTGACTCTCTACGGCTCGACGAGGACGGCGACGCCGAGAACGACGTCGTACCCAACGAATCAGCAATCCGAACCACCGCCGACAACGACGTCGTTCGAAGAAGCGAAATTGAATCTTCAGATGAGGAGAACGAAGACGACGACCAGACCCGAGGCGTGAGGGAAGATTTGGACGAATTCAAGCAGACGCTGACCCGCCAATTTTGGGGGGTCGCGTCATTCCTCGCTCCGCTTCCGCCCCCACCGCCTCCGCCGTACCCTTCATTGCCATCCAGTAATCGAACGGATCCTCCCGACATCGGTCGATCCGAACCGCCCGATCAATTGGCGATGGATTTTGGTGAAGGAGAAGAGGAGGAGGAGGAATTGGAGGAGGATTGCGCCGTAGGGGTCACTGAGGAGGTGCTGGCGTTCGCGAGGAACATCGCGATGCATCCGGAGACGTGGCTGGACTTTCCTCTCGACGAAGAGGAGGATTTGGGCGATTTTGACATGTCTGATGCCCAGCAAGAGCATGCTACGGTGATTGAACATCTTGCACCAAGATTAGCTGCTTTGAGATTTGAACTCTGTCCGTGCCATATGAGTGAGAGTTACTTCTGGAAAGTCTATTTTGTTCTTCTGCATTCGAGGCTCAATAAGCAGGATGCCGAGATCTTGTCCACACCCCAAGTAATGGAAGCCAGGGCAATGTGGATGCGGGAGCTACATTGTCAGACTGCATTAGAAGATGATCAATTTGAAAGAAACCCTTCTTTTTTAAAAGACCATTCCAATATACTACACGAAGATTGTGCTCCAGTCTCATCCTGTAGTGTTTATTACGAGGACAAGGTGCTAAGGACATATCCTTATGAATCAACAATGTCCCCCATGGCTTATGAGACTGAGAAGCACCCAGTTGAGAGTGATGAAATCCCATTTATTGATAAGTCTGTTATTGAAGAAAAGCCCATCATTAAGAGCGAGGACAAGAATCTAACAGTTGGTCCATCCTCGAAGATGCTAGATAATATCTATGAAAATGATGACGATGATGACTGGCCAGAGGAAGATTCTGAATTAGATGGGCATAGTGGAACAGCTATTCATGCAATCAATGAAGACGACATATCTTTCAGTGATCTAGAGGACGATGATTTCCTTGCATCCACAAAACTCAAGATTGTCTCAAAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

402

Amino Acids

45.69

Weight (kDa)

4.15

Isoelectric Point (pI)

69.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 169 - 224 5.4e-14 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 113
AatII GACGTC 2 cut(s) 72, 117
AccB7I CCANNNNNTGG 1 cut(s) 700
AccII CGCG 3 cut(s) 239, 440, 452
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 8 cut(s) 102, 220, 254, 260, 269, 275, 338, 724
AclWI GGATC 3 cut(s) 305, 318, 324
AcoI YGGCCR 1 cut(s) 1067
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 126
AcyI GRCGYC 3 cut(s) 57, 69, 114
AfaI GTAC 2 cut(s) 75, 281
AfiI CCNNNNNNNGG 3 cut(s) 229, 323, 700
AflIII ACRYGT 1 cut(s) 516
AgsI TTSAA 6 cut(s) 137, 205, 554, 590, 767, 968
AjiI CACGTC 1 cut(s) 468
AjnI CCWGG 1 cut(s) 707
AloI GAACNNNNNNTCC 2 cut(s) 298, 330
AluBI AGCT 3 cut(s) 575, 730, 1109
AluI AGCT 3 cut(s) 575, 730, 1109
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 4 cut(s) 458, 832, 902, 1198
AlwI GGATC 3 cut(s) 305, 318, 324
AlwNI CAGNNNCTG 1 cut(s) 214
Ama87I CYCGRG 1 cut(s) 174
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 1 cut(s) 1067
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 1 cut(s) 200
Asp700I GAANNNNTTC 1 cut(s) 775
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 1013
AsuHPI GGTGA 2 cut(s) 377, 559
AsuII TTCGAA 1 cut(s) 121
AvaI CYCGRG 1 cut(s) 174
AvaII GGWCC 1 cut(s) 1013
BalI TGGCCA 1 cut(s) 1069
BamHI GGATCC 1 cut(s) 310
BbsI GAAGAC 2 cut(s) 165, 1134
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 1 cut(s) 562
BccI CCATC 5 cut(s) 301, 349, 986, 1024, 1085
BceAI ACGGC 4 cut(s) 53, 67, 262, 392
BcgI CGANNNNNNTGC 2 cut(s) 800, 834
BciT130I CCWGG 1 cut(s) 709
BclI TGATCA 1 cut(s) 757
BcoDI GTCTC 4 cut(s) 458, 832, 902, 1198
BfaI CTAG 2 cut(s) 1031, 1151
BfmI CTRYAG 1 cut(s) 835
BglII AGATCT 1 cut(s) 678
BisI GCNGC 1 cut(s) 576
BlsI GCNGC 1 cut(s) 577
Bme1390I CCNGG 1 cut(s) 709
Bme18I GGWCC 1 cut(s) 1013
BmeT110I CYCGRG 1 cut(s) 174
BmgBI CACGTC 1 cut(s) 468
BmgT120I GGNCC 1 cut(s) 1013
BmiI GGNNCC 1 cut(s) 312
BmrFI CCNGG 1 cut(s) 709
BmrI ACTGGG 1 cut(s) 917
BmsI GCATC 7 cut(s) 444, 466, 514, 661, 711, 1017, 1181
BmuI ACTGGG 1 cut(s) 917
BoxI GACNNNNGTC 1 cut(s) 324
BpiI GAAGAC 2 cut(s) 165, 1134
BpmI CTGGAG 1 cut(s) 807
Bpu10I CCTNAGC 1 cut(s) 863
Bpu14I TTCGAA 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 1169
BsaHI GRCGYC 3 cut(s) 57, 69, 114
BsaJI CCNNGG 3 cut(s) 175, 708, 897
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 2 cut(s) 298, 328
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 323, 700
Bse1I ACTGG 4 cut(s) 297, 824, 923, 1070
Bse3DI GCAATG 2 cut(s) 287, 720
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 1 cut(s) 709
BseDI CCNNGG 3 cut(s) 175, 708, 897
BseGI GGATG 7 cut(s) 293, 457, 676, 726, 830, 1016, 1172
BseLI CCNNNNNNNGG 3 cut(s) 229, 323, 700
BseMI GCAATG 2 cut(s) 287, 720
BseMII CTCAG 2 cut(s) 411, 903
BseNI ACTGG 4 cut(s) 297, 824, 923, 1070
BseRI GAGGAG 7 cut(s) 164, 392, 395, 398, 410, 437, 509
BseXI GCAGC 1 cut(s) 562
BseYI CCCAGC 1 cut(s) 528
Bsh1236I CGCG 3 cut(s) 239, 440, 452
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 1 cut(s) 1069
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 823
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 1 cut(s) 461
BslFI GGGAC 1 cut(s) 877
BslI CCNNNNNNNGG 3 cut(s) 229, 323, 700
BsmAI GTCTC 4 cut(s) 458, 832, 902, 1198
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 877
BsmI GAATGC 1 cut(s) 649
BsnI GGCC 1 cut(s) 1069
BsoBI CYCGRG 1 cut(s) 174
Bsp119I TTCGAA 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 823
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 6 cut(s) 310, 329, 343, 678, 757, 1147
Bsp19I CCATGG 1 cut(s) 897
Bsp68I TCGCGA 2 cut(s) 440, 452
BspACI CCGC 8 cut(s) 102, 220, 254, 260, 269, 275, 338, 724
BspANI GGCC 1 cut(s) 1069
BspCNI CTCAG 2 cut(s) 412, 904
BspEI TCCGGA 1 cut(s) 460
BspFNI CGCG 3 cut(s) 239, 440, 452
BspLI GGNNCC 1 cut(s) 312
BspPI GGATC 3 cut(s) 305, 318, 324
BspT104I TTCGAA 1 cut(s) 121
BsrDI GCAATG 2 cut(s) 287, 720
BsrI ACTGG 4 cut(s) 297, 824, 923, 1070
BssECI CCNNGG 3 cut(s) 175, 708, 897
BssMI GATC 6 cut(s) 310, 329, 343, 678, 757, 1147
BssNI GRCGYC 3 cut(s) 57, 69, 114
BssT1I CCWWGG 1 cut(s) 897
Bst2UI CCWGG 1 cut(s) 709
Bst4CI ACNGT 2 cut(s) 547, 1009
Bst6I CTCTTC 2 cut(s) 369, 486
BstACI GRCGYC 3 cut(s) 57, 69, 114
BstBI TTCGAA 1 cut(s) 121
BstC8I GCNNGC 4 cut(s) 12, 16, 432, 540
BstDEI CTNAG 3 cut(s) 420, 863, 912
BstDSI CCRYGG 1 cut(s) 897
BstF5I GGATG 7 cut(s) 293, 457, 676, 726, 830, 1016, 1172
BstFNI CGCG 3 cut(s) 239, 440, 452
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 6 cut(s) 313, 332, 346, 681, 760, 1150
BstMAI GTCTC 4 cut(s) 458, 832, 902, 1198
BstMBI GATC 6 cut(s) 310, 329, 343, 678, 757, 1147
BstMCI CGRYCG 1 cut(s) 328
BstMWI GCNNNNNNNGC 4 cut(s) 268, 655, 664, 1115
BstNI CCWGG 1 cut(s) 709
BstNSI RCATGY 2 cut(s) 520, 542
BstPAI GACNNNNGTC 1 cut(s) 324
BstSCI CCNGG 1 cut(s) 707
BstSFI CTRYAG 1 cut(s) 835
BstUI CGCG 3 cut(s) 239, 440, 452
BstV1I GCAGC 1 cut(s) 562
BstV2I GAAGAC 2 cut(s) 165, 1134
BstX2I RGATCY 2 cut(s) 310, 678
BstYI RGATCY 2 cut(s) 310, 678
BsuRI GGCC 1 cut(s) 1069
BtgI CCRYGG 1 cut(s) 897
BtgZI GCGATG 3 cut(s) 368, 433, 467
BtrI CACGTC 1 cut(s) 468
BtsCI GGATG 7 cut(s) 293, 457, 676, 726, 830, 1016, 1172
BtsIMutI CAGTG 2 cut(s) 417, 1150
BtuMI TCGCGA 2 cut(s) 440, 452
Cac8I GCNNGC 4 cut(s) 12, 16, 432, 540
CaiI CAGNNNCTG 1 cut(s) 214
CfoI GCGC 1 cut(s) 407
Cfr13I GGNCC 1 cut(s) 1013
CseI GACGC 3 cut(s) 65, 220, 228
Csp6I GTAC 2 cut(s) 74, 280
CviAII CATG 5 cut(s) 6, 517, 539, 898, 1115
CviQI GTAC 2 cut(s) 74, 280
DdeI CTNAG 3 cut(s) 420, 863, 912
DpnI GATC 6 cut(s) 312, 331, 345, 680, 759, 1149
DpnII GATC 6 cut(s) 310, 329, 343, 678, 757, 1147
DraI TTTAAA 1 cut(s) 786
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
EaeI YGGCCR 1 cut(s) 1067
Eam1104I CTCTTC 2 cut(s) 369, 486
EarI CTCTTC 2 cut(s) 369, 486
EciI GGCGGA 2 cut(s) 249, 264
Eco130I CCWWGG 1 cut(s) 897
Eco47I GGWCC 1 cut(s) 1013
Eco57I CTGAAG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 174
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 707
EcoT14I CCWWGG 1 cut(s) 897
EcoT22I ATGCAT 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 897
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 5 cut(s) 9, 520, 542, 901, 1118
FalI AAGNNNNNCTT 2 cut(s) 760, 792
FaqI GGGAC 1 cut(s) 877
FatI CATG 5 cut(s) 5, 516, 538, 897, 1114
FauI CCCGC 2 cut(s) 227, 717
FauNDI CATATG 1 cut(s) 606
FbaI TGATCA 1 cut(s) 757
Fnu4HI GCNGC 1 cut(s) 576
FokI GGATG 7 cut(s) 280, 444, 683, 733, 817, 1003, 1159
Fsp4HI GCNGC 1 cut(s) 576
FspBI CTAG 2 cut(s) 1031, 1151
GlaI GCGC 1 cut(s) 406
GluI GCNGC 1 cut(s) 576
GsaI CCCAGC 1 cut(s) 532
GsuI CTGGAG 1 cut(s) 807
HaeIII GGCC 1 cut(s) 1069
HapII CCGG 1 cut(s) 461
HgaI GACGC 3 cut(s) 65, 220, 228
HhaI GCGC 1 cut(s) 407
Hin1I GRCGYC 3 cut(s) 57, 69, 114
Hin1II CATG 5 cut(s) 9, 520, 542, 901, 1118
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HinfI GANTC 6 cut(s) 29, 83, 137, 881, 1000, 1079
HpaII CCGG 1 cut(s) 461
HphI GGTGA 2 cut(s) 377, 559
Hpy166II GTNNAC 1 cut(s) 687
Hpy188I TCNGA 6 cut(s) 95, 145, 334, 523, 741, 1084
Hpy188III TCNNGA 8 cut(s) 317, 439, 451, 461, 485, 625, 1151, 1186
Hpy8I GTNNAC 1 cut(s) 687
Hpy99I CGWCG 8 cut(s) 47, 59, 71, 74, 116, 119, 167, 491
HpyAV CCTTC 3 cut(s) 294, 362, 786
HpyCH4III ACNGT 2 cut(s) 547, 1009
HpyCH4IV ACGT 3 cut(s) 69, 114, 467
HpyCH4V TGCA 6 cut(s) 457, 563, 649, 746, 1118, 1172
HpyF10VI GCNNNNNNNGC 4 cut(s) 268, 655, 664, 1115
HpyF3I CTNAG 3 cut(s) 420, 863, 912
HpySE526I ACGT 3 cut(s) 69, 114, 467
Hsp92I GRCGYC 3 cut(s) 57, 69, 114
Hsp92II CATG 5 cut(s) 9, 520, 542, 901, 1118
HspAI GCGC 1 cut(s) 405
Kpn2I TCCGGA 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 757
Kzo9I GATC 6 cut(s) 310, 329, 343, 678, 757, 1147
LmnI GCTCC 4 cut(s) 23, 256, 727, 826
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 7 cut(s) 444, 466, 514, 661, 711, 1017, 1181
MaeI CTAG 2 cut(s) 1031, 1151
MaeII ACGT 3 cut(s) 69, 114, 467
MaeIII GTNAC 2 cut(s) 415, 617
MalI GATC 6 cut(s) 312, 331, 345, 680, 759, 1149
MboI GATC 6 cut(s) 310, 329, 343, 678, 757, 1147
MfeI CAATTG 1 cut(s) 347
MflI RGATCY 2 cut(s) 310, 678
MhlI GDGCHC 1 cut(s) 823
MlsI TGGCCA 1 cut(s) 1069
MluCI AATT 7 cut(s) 132, 200, 224, 347, 389, 761, 1085
MluNI TGGCCA 1 cut(s) 1069
MlyI GAGTC 1 cut(s) 23
Mox20I TGGCCA 1 cut(s) 1069
Mph1103I ATGCAT 1 cut(s) 459
MroI TCCGGA 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 775
MscI TGGCCA 1 cut(s) 1069
MseI TTAA 2 cut(s) 785, 984
MslI CAYNNNNRTG 2 cut(s) 452, 609
Msp20I TGGCCA 1 cut(s) 1069
MspI CCGG 1 cut(s) 461
MspR9I CCNGG 1 cut(s) 709
MunI CAATTG 1 cut(s) 347
Mva1269I GAATGC 1 cut(s) 649
MvaI CCWGG 1 cut(s) 709
MvnI CGCG 3 cut(s) 239, 440, 452
MwoI GCNNNNNNNGC 4 cut(s) 268, 655, 664, 1115
NcoI CCATGG 1 cut(s) 897
NdeI CATATG 1 cut(s) 606
NdeII GATC 6 cut(s) 310, 329, 343, 678, 757, 1147
NlaIII CATG 5 cut(s) 9, 520, 542, 901, 1118
NlaIV GGNNCC 1 cut(s) 312
NmeAIII GCCGAG 2 cut(s) 85, 700
NmuCI GTSAC 1 cut(s) 415
NruI TCGCGA 2 cut(s) 440, 452
NsiI ATGCAT 1 cut(s) 459
NspI RCATGY 2 cut(s) 520, 542
NspV TTCGAA 1 cut(s) 121
PaeI GCATGC 1 cut(s) 542
PciI ACATGT 1 cut(s) 516
PcsI WCGNNNNNNNCGW 6 cut(s) 51, 57, 63, 78, 162, 330
PctI GAATGC 1 cut(s) 649
PdmI GAANNNNTTC 1 cut(s) 775
PfeI GAWTC 5 cut(s) 83, 137, 881, 1000, 1079
PflMI CCANNNNNTGG 1 cut(s) 700
PkrI GCNGC 1 cut(s) 577
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PscI ACATGT 1 cut(s) 516
PshAI GACNNNNGTC 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 707
PspFI CCCAGC 1 cut(s) 528
PspGI CCWGG 1 cut(s) 707
PspN4I GGNNCC 1 cut(s) 312
PspPI GGNCC 1 cut(s) 1013
PstNI CAGNNNCTG 1 cut(s) 214
PsuI RGATCY 2 cut(s) 310, 678
RruI TCGCGA 2 cut(s) 440, 452
RsaI GTAC 2 cut(s) 75, 281
RsaNI GTAC 2 cut(s) 74, 280
RseI CAYNNNNRTG 2 cut(s) 452, 609
SaqAI TTAA 2 cut(s) 785, 984
SatI GCNGC 1 cut(s) 576
Sau3AI GATC 6 cut(s) 310, 329, 343, 678, 757, 1147
Sau96I GGNCC 1 cut(s) 1013
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 1 cut(s) 709
SduI GDGCHC 1 cut(s) 823
SetI ASST 8 cut(s) 72, 117, 429, 470, 577, 732, 861, 1111
SfaNI GCATC 7 cut(s) 444, 466, 514, 661, 711, 1017, 1181
SfcI CTRYAG 1 cut(s) 835
SfuI TTCGAA 1 cut(s) 121
SinI GGWCC 1 cut(s) 1013
SmiMI CAYNNNNRTG 2 cut(s) 452, 609
SmlI CTYRAG 1 cut(s) 1184
SmoI CTYRAG 1 cut(s) 1184
SphI GCATGC 1 cut(s) 542
Sse9I AATT 7 cut(s) 132, 200, 224, 347, 389, 761, 1085
SsiI CCGC 8 cut(s) 102, 220, 254, 260, 269, 275, 338, 724
SspMI CTAG 2 cut(s) 1031, 1151
StyD4I CCNGG 1 cut(s) 707
StyI CCWWGG 1 cut(s) 897
TaaI ACNGT 2 cut(s) 547, 1009
TaiI ACGT 3 cut(s) 72, 117, 470
TaqI TCGA 7 cut(s) 42, 121, 304, 328, 486, 653, 1022
TaqII GACCGA 1 cut(s) 314
TasI AATT 7 cut(s) 132, 200, 224, 347, 389, 761, 1085
TfiI GAWTC 5 cut(s) 83, 137, 881, 1000, 1079
Tru1I TTAA 2 cut(s) 785, 984
Tru9I TTAA 2 cut(s) 785, 984
TscAI CASTG 2 cut(s) 424, 1150
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 6 cut(s) 276, 894, 951, 1059, 1103, 1140
TspGWI ACGGA 2 cut(s) 323, 588
TspRI CASTG 2 cut(s) 424, 1150
Van91I CCANNNNNTGG 1 cut(s) 700
VpaK11BI GGWCC 1 cut(s) 1013
XapI RAATTY 1 cut(s) 200
XbaI TCTAGA 1 cut(s) 1150
XceI RCATGY 2 cut(s) 520, 542
XmnI GAANNNNTTC 1 cut(s) 775
XspI CTAG 2 cut(s) 1031, 1151
ZraI GACGTC 2 cut(s) 70, 115
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.