Rroxscaffold_7G00159050

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012893
Physical Location & Seq
Reverse (-)
2346985 .. 2349107
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159050.1

Sequence Viewer

Length: 1239 bp
ATGGAACTCGGGCTCGTCACGCAGGCCTTGCATCGAAGAAAGAAATGGCTTCTGATACTCGCGGCTCTCGGCGCGTCCGGTTACGGCGCCTATAGGGTTTACAATTCGCCCTCTTTGGTCCAGAAGCGGAAGAGGTTCATGAAGGTCGTCGGAGCCCTAATTTCTATGGCGGAGATGGCGTCTGATTCTGCCGAGACTGTCAGCATTGTCTCCAACGACTTGAAGGAGTTTCTGAACTCCGATTCCGACGAAATGCCCAAAAGTCTGAAGCAGATTTCCAAGATTGCGAGGTCGGAGGAGCTCTCTGCGTCGTTGCAGAGGTTGACGGAGGCTATGACGATCGGGGTTTGCCGAGGGTTTGAGTTTGAGACGGGGAATGGGGTTGGATCGGAGAAGGGTTTGGGGAATTCGAGCTATTCAGAGAGAGTTATGGAGAAGGTTTTCTCTGAAGCCGGGACCGGGTTCGTTTCGGTTGTACTCGGAAGCTTTGCAAGGAATATGGTTATGGGGTTTTACTCAAATGAGCATTCAGGTCAGGATTCGAGTTCTTCAGATGTGAATAGATGGGTCGATGTGGTTTGTAGTGATCGAAGTAAGGAGCTGATAGCGAATTGCATACAAGTTTTCGTGTCTACTGCGGTTGCTGCGTACCTAGAGAAAACGAAGGATGTCAATTTCTGTGATGACATGTTTGGTGGAATGACCAATTCCAAGCATCAGGAGAAGGTCAGGGAGCTCTTGGTTTCTGTTTGCAATGGTGCTGTGGAGACTGTTGTGAAAACTTCTCATCAAGTTTTGACGAGTTCTGGTTCGAATCCGAGCTCAAATTCGGGGACTTGGTCAGTTGTTGATCATGAAGAAGGTGTGAGTGGAGCTAGGGATGAGTGCCTTGAGCAAGAAGCAAAGCAGCCGAAGGAGGGAAGTTCACTTGAGGCTATTCAGAATAGCGAGTGGATTGGAAAGGTTTCGTCTACATTGGCAGTGCCGCGCAATAGGAAGTTTGTTCGTGAGGTGACTGGGAGGGTGACATTCGAGACTGTAAGATCTTTTGTTGAATTCATGTTGTGGACACTACTAGAGGGGTTGAAAAGAACTGTTAGTGTGGTTCATGGGGAGATTGTAGGTAGGGGGTTGCAAGTTATCAGATATTTTGGTGCAAAGTCTTCGGCTATTGTTACCATATGTCTTGCTTTGCACTTGTATGTTCTAGGAGGTAGTAGAGTTCTCTTGACTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

412

Amino Acids

44.94

Weight (kDa)

7.05

Isoelectric Point (pI)

30.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10150 AT1G59510
fragaria_vesca FvH4_2g32762
malus_domestica MD08G1079200.v1.1 MD15G1066400.v1.1
prunus_persica Prupe.1G420200_v2.0.a1
pyrus_communis pycom15g06260
rosa_chinensis RchiOBHm_Chr6g0309911
rosa_laevigata RLG00000010524
rosa_multiflora Rmu_co7980562.1_g000001 Rmu_sc0000294.1_g000005
rosa_roxburghii Rroxscaffold_7G00159050
rosa_rugosa Rorug06G0375800
rosa_samantha Rh6AG489700 Rh6BG499100 Rh6CG504000 Rh6DG490200
rosa_wichuraiana Rw6G042620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccI GTMKAC 2 cut(s) 632, 971
AccII CGCG 3 cut(s) 62, 74, 988
AciI CCGC 5 cut(s) 62, 127, 170, 638, 986
AclWI GGATC 1 cut(s) 394
AcsI RAATTY 3 cut(s) 406, 826, 1055
AcuI CTGAAG 3 cut(s) 287, 468, 534
AcyI GRCGYC 2 cut(s) 87, 179
AfaI GTAC 2 cut(s) 477, 650
AfiI CCNNNNNNNGG 2 cut(s) 459, 917
AflIII ACRYGT 1 cut(s) 687
AgsI TTSAA 3 cut(s) 223, 1055, 1087
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 7 cut(s) 301, 414, 486, 601, 736, 822, 875
AluI AGCT 7 cut(s) 301, 414, 486, 601, 736, 822, 875
Alw21I GWGCWC 3 cut(s) 303, 738, 824
Alw26I GTCTC 5 cut(s) 188, 214, 362, 761, 1028
AlwI GGATC 1 cut(s) 394
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 1 cut(s) 24
ApeKI GCWGC 2 cut(s) 644, 907
ApoI RAATTY 3 cut(s) 406, 826, 1055
Asp700I GAANNNNTTC 3 cut(s) 134, 440, 964
AspLEI GCGC 3 cut(s) 74, 89, 990
AspS9I GGNCC 2 cut(s) 118, 456
AsuC2I CCSGG 2 cut(s) 454, 460
AsuHPI GGTGA 2 cut(s) 1024, 1036
AsuII TTCGAA 1 cut(s) 812
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 2 cut(s) 118, 456
BanI GGYRCC 1 cut(s) 86
BanII GRGCYC 5 cut(s) 15, 157, 303, 738, 824
BbsI GAAGAC 1 cut(s) 1155
Bbv12I GWGCWC 3 cut(s) 303, 738, 824
BbvI GCAGC 2 cut(s) 631, 919
BccI CCATC 2 cut(s) 169, 558
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 2 cut(s) 1146, 1180
BclI TGATCA 1 cut(s) 850
BcnI CCSGG 2 cut(s) 454, 460
BcoDI GTCTC 5 cut(s) 188, 214, 362, 761, 1028
BfaI CTAG 5 cut(s) 653, 876, 1076, 1208, 1237
BfmI CTRYAG 1 cut(s) 91
BfoI RGCGCY 1 cut(s) 90
BglII AGATCT 1 cut(s) 1043
BisI GCNGC 4 cut(s) 63, 645, 908, 986
BlsI GCNGC 4 cut(s) 64, 646, 909, 987
Bme1390I CCNGG 2 cut(s) 454, 460
Bme18I GGWCC 2 cut(s) 118, 456
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 118, 456
BmiI GGNNCC 3 cut(s) 88, 154, 457
BmrFI CCNGG 2 cut(s) 454, 460
BmrI ACTGGG 1 cut(s) 1026
BmsI GCATC 2 cut(s) 40, 724
BmuI ACTGGG 1 cut(s) 1026
BpiI GAAGAC 1 cut(s) 1155
BplI GAGNNNNNCTC 2 cut(s) 287, 319
Bpu14I TTCGAA 1 cut(s) 812
BpuEI CTTGAG 2 cut(s) 911, 950
BpuMI CCSGG 2 cut(s) 454, 460
BsaHI GRCGYC 2 cut(s) 87, 179
BsaJI CCNNGG 1 cut(s) 352
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 2 cut(s) 459, 917
Bse1I ACTGG 1 cut(s) 1021
Bse3DI GCAATG 1 cut(s) 760
BseDI CCNNGG 1 cut(s) 352
BseGI GGATG 2 cut(s) 673, 886
BseLI CCNNNNNNNGG 2 cut(s) 459, 917
BseMI GCAATG 1 cut(s) 760
BseNI ACTGG 1 cut(s) 1021
BseRI GAGGAG 1 cut(s) 311
BseXI GCAGC 2 cut(s) 631, 919
Bsh1236I CGCG 3 cut(s) 62, 74, 988
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 1 cut(s) 26
BshNI GGYRCC 1 cut(s) 86
BsiEI CGRYCG 1 cut(s) 342
BsiHKAI GWGCWC 3 cut(s) 303, 738, 824
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 3 cut(s) 78, 453, 459
BslFI GGGAC 2 cut(s) 469, 847
BslI CCNNNNNNNGG 2 cut(s) 459, 917
BsmAI GTCTC 5 cut(s) 188, 214, 362, 761, 1028
BsmBI CGTCTC 1 cut(s) 362
BsmFI GGGAC 2 cut(s) 469, 847
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 1 cut(s) 26
BsoBI CYCGRG 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 812
Bsp1286I GDGCHC 5 cut(s) 15, 157, 303, 738, 824
Bsp143I GATC 5 cut(s) 339, 386, 586, 850, 1043
BspACI CCGC 5 cut(s) 62, 127, 170, 638, 986
BspANI GGCC 1 cut(s) 26
BspFNI CGCG 3 cut(s) 62, 74, 988
BspHI TCATGA 2 cut(s) 138, 853
BspLI GGNNCC 3 cut(s) 88, 154, 457
BspPI GGATC 1 cut(s) 394
BspT104I TTCGAA 1 cut(s) 812
BspT107I GGYRCC 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 760
BsrI ACTGG 1 cut(s) 1021
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 5 cut(s) 339, 386, 586, 850, 1043
BssNI GRCGYC 2 cut(s) 87, 179
Bst4CI ACNGT 4 cut(s) 199, 772, 1039, 1096
Bst6I CTCTTC 1 cut(s) 125
BstACI GRCGYC 2 cut(s) 87, 179
BstAPI GCANNNNNTGC 1 cut(s) 28
BstBI TTCGAA 1 cut(s) 812
BstC8I GCNNGC 1 cut(s) 24
BstF5I GGATG 2 cut(s) 673, 886
BstFNI CGCG 3 cut(s) 62, 74, 988
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 3 cut(s) 74, 89, 990
BstKTI GATC 5 cut(s) 342, 389, 589, 853, 1046
BstMAI GTCTC 5 cut(s) 188, 214, 362, 761, 1028
BstMBI GATC 5 cut(s) 339, 386, 586, 850, 1043
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 5 cut(s) 19, 28, 71, 176, 644
BstNSI RCATGY 1 cut(s) 691
BstSCI CCNGG 2 cut(s) 452, 458
BstSFI CTRYAG 1 cut(s) 91
BstUI CGCG 3 cut(s) 62, 74, 988
BstV1I GCAGC 2 cut(s) 631, 919
BstV2I GAAGAC 1 cut(s) 1155
BstX2I RGATCY 1 cut(s) 1043
BstYI RGATCY 1 cut(s) 1043
BsuRI GGCC 1 cut(s) 26
BtsCI GGATG 2 cut(s) 673, 886
BtsI GCAGTG 1 cut(s) 987
BtsIMutI CAGTG 1 cut(s) 987
Cac8I GCNNGC 1 cut(s) 24
CciI TCATGA 2 cut(s) 138, 853
CfoI GCGC 3 cut(s) 74, 89, 990
Cfr13I GGNCC 2 cut(s) 118, 456
CseI GACGC 3 cut(s) 63, 168, 297
Csp6I GTAC 2 cut(s) 476, 649
CviAII CATG 5 cut(s) 139, 688, 854, 1060, 1109
CviQI GTAC 2 cut(s) 476, 649
DinI GGCGCC 1 cut(s) 88
DpnI GATC 5 cut(s) 341, 388, 588, 852, 1045
DpnII GATC 5 cut(s) 339, 386, 586, 850, 1043
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 3 cut(s) 301, 736, 822
Eco147I AGGCCT 1 cut(s) 26
Eco24I GRGCYC 5 cut(s) 15, 157, 303, 738, 824
Eco47I GGWCC 2 cut(s) 118, 456
Eco53kI GAGCTC 3 cut(s) 301, 736, 822
Eco57I CTGAAG 3 cut(s) 287, 468, 534
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 3 cut(s) 301, 736, 822
EcoRI GAATTC 2 cut(s) 406, 1055
EcoT38I GRGCYC 5 cut(s) 15, 157, 303, 738, 824
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
Esp3I CGTCTC 1 cut(s) 362
FaeI CATG 5 cut(s) 142, 691, 857, 1063, 1112
FaqI GGGAC 2 cut(s) 469, 847
FatI CATG 5 cut(s) 138, 687, 853, 1059, 1108
FauNDI CATATG 1 cut(s) 1181
FbaI TGATCA 1 cut(s) 850
FblI GTMKAC 2 cut(s) 632, 971
Fnu4HI GCNGC 4 cut(s) 63, 645, 908, 986
FokI GGATG 2 cut(s) 680, 893
FriOI GRGCYC 5 cut(s) 15, 157, 303, 738, 824
Fsp4HI GCNGC 4 cut(s) 63, 645, 908, 986
FspBI CTAG 5 cut(s) 653, 876, 1076, 1208, 1237
GlaI GCGC 3 cut(s) 73, 88, 989
GluI GCNGC 4 cut(s) 63, 645, 908, 986
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 1 cut(s) 26
HapII CCGG 3 cut(s) 78, 453, 459
HgaI GACGC 3 cut(s) 63, 168, 297
HhaI GCGC 3 cut(s) 74, 89, 990
Hin1I GRCGYC 2 cut(s) 87, 179
Hin1II CATG 5 cut(s) 142, 691, 857, 1063, 1112
Hin6I GCGC 3 cut(s) 72, 87, 988
HinP1I GCGC 3 cut(s) 72, 87, 988
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 4 cut(s) 185, 242, 539, 814
HpaII CCGG 3 cut(s) 78, 453, 459
HphI GGTGA 2 cut(s) 1024, 1036
Hpy166II GTNNAC 6 cut(s) 100, 324, 633, 926, 972, 1068
Hpy188III TCNNGA 8 cut(s) 121, 139, 536, 719, 854, 1007, 1033, 1228
Hpy8I GTNNAC 6 cut(s) 100, 324, 633, 926, 972, 1068
Hpy99I CGWCG 3 cut(s) 152, 251, 313
HpyAV CCTTC 8 cut(s) 136, 217, 388, 430, 658, 718, 854, 907
HpyCH4III ACNGT 4 cut(s) 199, 772, 1039, 1096
HpyCH4V TGCA 8 cut(s) 31, 316, 491, 615, 753, 1135, 1157, 1195
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 28, 71, 176, 644
Hsp92I GRCGYC 2 cut(s) 87, 179
Hsp92II CATG 5 cut(s) 142, 691, 857, 1063, 1112
HspAI GCGC 3 cut(s) 72, 87, 988
KasI GGCGCC 1 cut(s) 86
Ksp22I TGATCA 1 cut(s) 850
Kzo9I GATC 5 cut(s) 339, 386, 586, 850, 1043
LmnI GCTCC 5 cut(s) 152, 298, 598, 733, 872
Lsp1109I GCAGC 2 cut(s) 631, 919
LweI GCATC 2 cut(s) 40, 724
MaeI CTAG 5 cut(s) 653, 876, 1076, 1208, 1237
MaeIII GTNAC 5 cut(s) 16, 80, 1012, 1024, 1174
MalI GATC 5 cut(s) 341, 388, 588, 852, 1045
MboI GATC 5 cut(s) 339, 386, 586, 850, 1043
MboII GAAGA 5 cut(s) 48, 142, 540, 869, 1155
MflI RGATCY 1 cut(s) 1043
MhlI GDGCHC 5 cut(s) 15, 157, 303, 738, 824
MluCI AATT 8 cut(s) 103, 159, 406, 610, 673, 706, 826, 1055
Mly113I GGCGCC 1 cut(s) 87
MmeI TCCRAC 5 cut(s) 130, 237, 270, 273, 364
MroXI GAANNNNTTC 3 cut(s) 134, 440, 964
MslI CAYNNNNRTG 1 cut(s) 1200
MspI CCGG 3 cut(s) 78, 453, 459
MspR9I CCNGG 2 cut(s) 454, 460
Mva1269I GAATGC 1 cut(s) 526
MvnI CGCG 3 cut(s) 62, 74, 988
MwoI GCNNNNNNNGC 5 cut(s) 19, 28, 71, 176, 644
NarI GGCGCC 1 cut(s) 87
NciI CCSGG 2 cut(s) 454, 460
NdeI CATATG 1 cut(s) 1181
NdeII GATC 5 cut(s) 339, 386, 586, 850, 1043
NlaIII CATG 5 cut(s) 142, 691, 857, 1063, 1112
NlaIV GGNNCC 3 cut(s) 88, 154, 457
NmeAIII GCCGAG 3 cut(s) 48, 217, 377
NmuCI GTSAC 3 cut(s) 16, 1012, 1024
NspI RCATGY 1 cut(s) 691
NspV TTCGAA 1 cut(s) 812
PagI TCATGA 2 cut(s) 138, 853
PceI AGGCCT 1 cut(s) 26
PciI ACATGT 1 cut(s) 687
PctI GAATGC 1 cut(s) 526
PdmI GAANNNNTTC 3 cut(s) 134, 440, 964
PfeI GAWTC 4 cut(s) 185, 242, 539, 814
PflFI GACNNNGTC 1 cut(s) 838
PkrI GCNGC 4 cut(s) 64, 646, 909, 987
Ple19I CGATCG 1 cut(s) 342
PluTI GGCGCC 1 cut(s) 90
PscI ACATGT 1 cut(s) 687
Psp124BI GAGCTC 3 cut(s) 303, 738, 824
PspN4I GGNNCC 3 cut(s) 88, 154, 457
PspPI GGNCC 2 cut(s) 118, 456
PsuI RGATCY 1 cut(s) 1043
PsyI GACNNNGTC 1 cut(s) 838
PvuI CGATCG 1 cut(s) 342
RsaI GTAC 2 cut(s) 477, 650
RsaNI GTAC 2 cut(s) 476, 649
RseI CAYNNNNRTG 1 cut(s) 1200
SacI GAGCTC 3 cut(s) 303, 738, 824
SatI GCNGC 4 cut(s) 63, 645, 908, 986
Sau3AI GATC 5 cut(s) 339, 386, 586, 850, 1043
Sau96I GGNCC 2 cut(s) 118, 456
ScrFI CCNGG 2 cut(s) 454, 460
SduI GDGCHC 5 cut(s) 15, 157, 303, 738, 824
SfaNI GCATC 2 cut(s) 40, 724
SfcI CTRYAG 1 cut(s) 91
SfoI GGCGCC 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 812
SinI GGWCC 2 cut(s) 118, 456
SmiMI CAYNNNNRTG 1 cut(s) 1200
SmlI CTYRAG 2 cut(s) 890, 929
SmoI CTYRAG 2 cut(s) 890, 929
Sse9I AATT 8 cut(s) 103, 159, 406, 610, 673, 706, 826, 1055
SseBI AGGCCT 1 cut(s) 26
SsiI CCGC 5 cut(s) 62, 127, 170, 638, 986
SspDI GGCGCC 1 cut(s) 86
SspMI CTAG 5 cut(s) 653, 876, 1076, 1208, 1237
SstI GAGCTC 3 cut(s) 303, 738, 824
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 2 cut(s) 452, 458
TaaI ACNGT 4 cut(s) 199, 772, 1039, 1096
TaqI TCGA 7 cut(s) 34, 410, 542, 570, 589, 812, 1032
TasI AATT 8 cut(s) 103, 159, 406, 610, 673, 706, 826, 1055
TatI WGTACW 1 cut(s) 475
TauI GCSGC 2 cut(s) 65, 988
TfiI GAWTC 4 cut(s) 185, 242, 539, 814
TscAI CASTG 1 cut(s) 987
TseFI GTSAC 3 cut(s) 16, 1012, 1024
TseI GCWGC 2 cut(s) 644, 907
Tsp45I GTSAC 3 cut(s) 16, 1012, 1024
TspDTI ATGAA 5 cut(s) 127, 155, 870, 1048, 1097
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 1 cut(s) 987
Tth111I GACNNNGTC 1 cut(s) 838
VpaK11BI GGWCC 2 cut(s) 118, 456
XapI RAATTY 3 cut(s) 406, 826, 1055
XceI RCATGY 1 cut(s) 691
XmiI GTMKAC 2 cut(s) 632, 971
XmnI GAANNNNTTC 3 cut(s) 134, 440, 964
XspI CTAG 5 cut(s) 653, 876, 1076, 1208, 1237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.