RLG00000010524

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012893
Physical Location & Seq
Reverse (-)
2369938 .. 2371167
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010524

Sequence Viewer

Length: 1230 bp
ATGGAACTCGGGCTCGTCACGAAGGCCTTGCATCGAAGAAAGAAATGGCTTCTGATACTCGCGGCTCTCGGCGCGTCCGGTTACGGCGCCTACAGGGTTTACAATTCGCCCTCTTCGGTCCAGAAGCGAAAGAGGTTCATGAAGGTCGTCGGAGCCCTAATTTCTATGGCGGAGATGGCGTCTGATTCGGCCGAGACCGTCAGCATCGTCTCCAACGACTTGAAGGAGTTTCTGAACTCCGATTCCGACGAAATGCCCAAAAGTCTGAAGCAGATTTCCAAGATTGCGAGGTCGGAGGAGTTCTCTGCGTCGTTGCAGGGGTTGACGGAGGCTCTGACGATCGGGGTTTACCGAGGGTTTGAGTTTGAGACGAGGAATGGGGTTGGATCGGAGAAGGGTTTGGGCTTTTCAGAGAGAGTTATGGAGAAGGTTTTCTCTGAAGCCGGGACCGGGTTCGTTTCGGTTGTAGTTGGAAGCTTTGCAAGGAATATGGTTATGGGTTTTTACTCAAATGAGCATTCAGGTCAGGATTCGAGTTCTTCGGATGTGAATAGATGGGTTGATGCGGTTTGTAGTGATGGAAGTAAGGAGCTGATAGCGAATTGCATACAGGTTTTCGTGTCTACTGCGGTTGCGGCGTACCTAGACAAAACGAAGGATGTCAATTTCTGTGATGACATGTTTGGTGGAATGACCAATTCCAAGCATCAGGAGAAGGTCAGGGAGCTCTTGGTTTCTGTTTGCAATGGTGCTGTGGAGACTGTTGTGAAAACTTCTCATCAAGTTTTGACGAGTTCTGGTTCGAATCCGAGCTCAAATTCCGGGACTTGGTCAGTTGTTGATCATGATGAAGGTGTGAGTGGAGGTAGGGATGAGTGCCTTGAGCAAGAAGCAAAGCGGCCGAAGGAGGGAAGTTCACTGGAGGCTATTCAGAATAGCGAGTGGATTGGAAAGGTTTCGTCTACATTGGCAGTGCCGCGCAATAGGAAGTTTGTTCGTGACGTGACTGGGAGGGTGACATTCGAGACTGTAAGATCTTTTGTTGAATTCATGTTGTGGACACTGCTAGAGGGATTGAAAAGAACTGTTAGTGTGGTTCATGGGGAGATTGTGGGTAGGGGGTTGCAAGTTATCAGATATTTTGGTGCAAAGTCTTCGGCTATTGTCACCATATGTCTTGCTTTGCACTTGTATGTTCTAGGAGGTAGTAGAGTTCTCTTGACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

410

Amino Acids

44.51

Weight (kDa)

7.62

Isoelectric Point (pI)

29.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012893)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10150 AT1G59510
fragaria_vesca FvH4_2g32762
malus_domestica MD08G1079200.v1.1 MD15G1066400.v1.1
prunus_persica Prupe.1G420200_v2.0.a1
pyrus_communis pycom15g06260
rosa_chinensis RchiOBHm_Chr6g0309911
rosa_laevigata RLG00000010524
rosa_multiflora Rmu_co7980562.1_g000001 Rmu_sc0000294.1_g000005
rosa_roxburghii Rroxscaffold_7G00159050
rosa_rugosa Rorug06G0375800
rosa_samantha Rh6AG489700 Rh6BG499100 Rh6CG504000 Rh6DG490200
rosa_wichuraiana Rw6G042620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccI GTMKAC 2 cut(s) 623, 962
AccII CGCG 3 cut(s) 62, 74, 979
AciI CCGC 7 cut(s) 62, 170, 566, 629, 635, 898, 977
AclWI GGATC 1 cut(s) 394
AcoI YGGCCR 2 cut(s) 189, 899
AcsI RAATTY 2 cut(s) 817, 1046
AcuI CTGAAG 2 cut(s) 287, 459
AcyI GRCGYC 2 cut(s) 87, 179
AfaI GTAC 1 cut(s) 641
AfiI CCNNNNNNNGG 3 cut(s) 450, 828, 908
AflIII ACRYGT 1 cut(s) 678
AgsI TTSAA 3 cut(s) 223, 1046, 1078
AjiI CACGTC 1 cut(s) 1003
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 4 cut(s) 477, 592, 727, 813
AluI AGCT 4 cut(s) 477, 592, 727, 813
Alw21I GWGCWC 2 cut(s) 729, 815
Alw26I GTCTC 5 cut(s) 188, 214, 362, 752, 1019
AlwI GGATC 1 cut(s) 394
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 3 cut(s) 24, 189, 899
ApoI RAATTY 2 cut(s) 817, 1046
Asp700I GAANNNNTTC 2 cut(s) 431, 955
AspLEI GCGC 3 cut(s) 74, 89, 981
AspS9I GGNCC 2 cut(s) 118, 447
AsuC2I CCSGG 3 cut(s) 445, 451, 823
AsuHPI GGTGA 2 cut(s) 1027, 1159
AsuII TTCGAA 1 cut(s) 803
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 2 cut(s) 118, 447
BanI GGYRCC 1 cut(s) 86
BanII GRGCYC 4 cut(s) 15, 157, 729, 815
BbsI GAAGAC 1 cut(s) 1146
Bbv12I GWGCWC 2 cut(s) 729, 815
BccI CCATC 3 cut(s) 169, 549, 572
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 4 cut(s) 10, 44, 1137, 1171
BclI TGATCA 1 cut(s) 841
BcnI CCSGG 3 cut(s) 445, 451, 823
BcoDI GTCTC 5 cut(s) 188, 214, 362, 752, 1019
BfaI CTAG 3 cut(s) 644, 1067, 1199
BfmI CTRYAG 1 cut(s) 91
BfoI RGCGCY 1 cut(s) 90
BglII AGATCT 1 cut(s) 1034
BisI GCNGC 4 cut(s) 63, 636, 899, 977
BlsI GCNGC 4 cut(s) 64, 637, 900, 978
Bme1390I CCNGG 3 cut(s) 445, 451, 823
Bme18I GGWCC 2 cut(s) 118, 447
BmeT110I CYCGRG 1 cut(s) 8
BmgBI CACGTC 1 cut(s) 1003
BmgT120I GGNCC 2 cut(s) 118, 447
BmiI GGNNCC 3 cut(s) 88, 154, 448
BmrFI CCNGG 3 cut(s) 445, 451, 823
BmrI ACTGGG 1 cut(s) 1017
BmsI GCATC 4 cut(s) 40, 213, 553, 715
BmuI ACTGGG 1 cut(s) 1017
BpiI GAAGAC 1 cut(s) 1146
BplI GAGNNNNNCTC 2 cut(s) 287, 319
BpmI CTGGAG 1 cut(s) 941
Bpu14I TTCGAA 1 cut(s) 803
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 3 cut(s) 445, 451, 823
BsaHI GRCGYC 2 cut(s) 87, 179
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 352
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 3 cut(s) 450, 828, 908
Bse1I ACTGG 2 cut(s) 924, 1012
Bse3DI GCAATG 1 cut(s) 751
BseDI CCNNGG 1 cut(s) 352
BseGI GGATG 3 cut(s) 550, 664, 877
BseLI CCNNNNNNNGG 3 cut(s) 450, 828, 908
BseMI GCAATG 1 cut(s) 751
BseNI ACTGG 2 cut(s) 924, 1012
BseRI GAGGAG 1 cut(s) 311
BseX3I CGGCCG 2 cut(s) 189, 899
Bsh1236I CGCG 3 cut(s) 62, 74, 979
Bsh1285I CGRYCG 3 cut(s) 192, 342, 902
BshFI GGCC 3 cut(s) 26, 191, 901
BshNI GGYRCC 1 cut(s) 86
BsiEI CGRYCG 3 cut(s) 192, 342, 902
BsiHKAI GWGCWC 2 cut(s) 729, 815
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 4 cut(s) 78, 444, 450, 822
BslFI GGGAC 2 cut(s) 460, 838
BslI CCNNNNNNNGG 3 cut(s) 450, 828, 908
BsmAI GTCTC 5 cut(s) 188, 214, 362, 752, 1019
BsmBI CGTCTC 2 cut(s) 214, 362
BsmFI GGGAC 2 cut(s) 460, 838
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 3 cut(s) 26, 191, 901
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 803
Bsp1286I GDGCHC 4 cut(s) 15, 157, 729, 815
Bsp143I GATC 4 cut(s) 339, 386, 841, 1034
BspACI CCGC 7 cut(s) 62, 170, 566, 629, 635, 898, 977
BspANI GGCC 3 cut(s) 26, 191, 901
BspFNI CGCG 3 cut(s) 62, 74, 979
BspHI TCATGA 2 cut(s) 138, 844
BspLI GGNNCC 3 cut(s) 88, 154, 448
BspPI GGATC 1 cut(s) 394
BspT104I TTCGAA 1 cut(s) 803
BspT107I GGYRCC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 751
BsrI ACTGG 2 cut(s) 924, 1012
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 4 cut(s) 339, 386, 841, 1034
BssNI GRCGYC 2 cut(s) 87, 179
Bst4CI ACNGT 4 cut(s) 199, 763, 1030, 1087
Bst6I CTCTTC 1 cut(s) 118
BstACI GRCGYC 2 cut(s) 87, 179
BstBI TTCGAA 1 cut(s) 803
BstDEI CTNAG 1 cut(s) 1227
BstF5I GGATG 3 cut(s) 550, 664, 877
BstFNI CGCG 3 cut(s) 62, 74, 979
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 3 cut(s) 74, 89, 981
BstKTI GATC 4 cut(s) 342, 389, 844, 1037
BstMAI GTCTC 5 cut(s) 188, 214, 362, 752, 1019
BstMBI GATC 4 cut(s) 339, 386, 841, 1034
BstMCI CGRYCG 3 cut(s) 192, 342, 902
BstMWI GCNNNNNNNGC 3 cut(s) 71, 176, 635
BstNSI RCATGY 1 cut(s) 682
BstSCI CCNGG 3 cut(s) 443, 449, 821
BstSFI CTRYAG 1 cut(s) 91
BstUI CGCG 3 cut(s) 62, 74, 979
BstV2I GAAGAC 1 cut(s) 1146
BstX2I RGATCY 1 cut(s) 1034
BstYI RGATCY 1 cut(s) 1034
BstZI CGGCCG 2 cut(s) 189, 899
BsuRI GGCC 3 cut(s) 26, 191, 901
BtrI CACGTC 1 cut(s) 1003
BtsCI GGATG 3 cut(s) 550, 664, 877
BtsI GCAGTG 2 cut(s) 978, 1061
BtsIMutI CAGTG 3 cut(s) 917, 978, 1061
CciI TCATGA 2 cut(s) 138, 844
CfoI GCGC 3 cut(s) 74, 89, 981
Cfr13I GGNCC 2 cut(s) 118, 447
CseI GACGC 3 cut(s) 63, 168, 297
Csp6I GTAC 1 cut(s) 640
CviAII CATG 5 cut(s) 139, 679, 845, 1051, 1100
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 1 cut(s) 1227
DinI GGCGCC 1 cut(s) 88
DpnI GATC 4 cut(s) 341, 388, 843, 1036
DpnII GATC 4 cut(s) 339, 386, 841, 1034
EaeI YGGCCR 2 cut(s) 189, 899
EagI CGGCCG 2 cut(s) 189, 899
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 2 cut(s) 727, 813
EclXI CGGCCG 2 cut(s) 189, 899
Eco147I AGGCCT 1 cut(s) 26
Eco24I GRGCYC 4 cut(s) 15, 157, 729, 815
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 2 cut(s) 118, 447
Eco52I CGGCCG 2 cut(s) 189, 899
Eco53kI GAGCTC 2 cut(s) 727, 813
Eco57I CTGAAG 2 cut(s) 287, 459
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 2 cut(s) 727, 813
EcoRI GAATTC 1 cut(s) 1046
EcoT38I GRGCYC 4 cut(s) 15, 157, 729, 815
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
Esp3I CGTCTC 2 cut(s) 214, 362
FaeI CATG 5 cut(s) 142, 682, 848, 1054, 1103
FaqI GGGAC 2 cut(s) 460, 838
FatI CATG 5 cut(s) 138, 678, 844, 1050, 1099
FauNDI CATATG 1 cut(s) 1172
FbaI TGATCA 1 cut(s) 841
FblI GTMKAC 2 cut(s) 623, 962
Fnu4HI GCNGC 4 cut(s) 63, 636, 899, 977
FokI GGATG 3 cut(s) 557, 671, 884
FriOI GRGCYC 4 cut(s) 15, 157, 729, 815
Fsp4HI GCNGC 4 cut(s) 63, 636, 899, 977
FspBI CTAG 3 cut(s) 644, 1067, 1199
GlaI GCGC 3 cut(s) 73, 88, 980
GluI GCNGC 4 cut(s) 63, 636, 899, 977
GsuI CTGGAG 1 cut(s) 941
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 3 cut(s) 26, 191, 901
HapII CCGG 4 cut(s) 78, 444, 450, 822
HgaI GACGC 3 cut(s) 63, 168, 297
HhaI GCGC 3 cut(s) 74, 89, 981
Hin1I GRCGYC 2 cut(s) 87, 179
Hin1II CATG 5 cut(s) 142, 682, 848, 1054, 1103
Hin6I GCGC 3 cut(s) 72, 87, 979
HinP1I GCGC 3 cut(s) 72, 87, 979
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HindIII AAGCTT 1 cut(s) 475
HinfI GANTC 4 cut(s) 185, 242, 530, 805
HpaII CCGG 4 cut(s) 78, 444, 450, 822
HphI GGTGA 2 cut(s) 1027, 1159
Hpy166II GTNNAC 7 cut(s) 100, 324, 349, 624, 917, 963, 1059
Hpy188III TCNNGA 9 cut(s) 19, 121, 139, 527, 710, 845, 998, 1024, 1219
Hpy8I GTNNAC 7 cut(s) 100, 324, 349, 624, 917, 963, 1059
Hpy99I CGWCG 3 cut(s) 152, 251, 313
HpyAV CCTTC 9 cut(s) 16, 136, 217, 388, 421, 649, 709, 845, 898
HpyCH4III ACNGT 4 cut(s) 199, 763, 1030, 1087
HpyCH4IV ACGT 1 cut(s) 1002
HpyCH4V TGCA 8 cut(s) 31, 316, 482, 606, 744, 1126, 1148, 1186
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 176, 635
HpyF3I CTNAG 1 cut(s) 1227
HpySE526I ACGT 1 cut(s) 1002
Hsp92I GRCGYC 2 cut(s) 87, 179
Hsp92II CATG 5 cut(s) 142, 682, 848, 1054, 1103
HspAI GCGC 3 cut(s) 72, 87, 979
KasI GGCGCC 1 cut(s) 86
Ksp22I TGATCA 1 cut(s) 841
Kzo9I GATC 4 cut(s) 339, 386, 841, 1034
LmnI GCTCC 3 cut(s) 152, 589, 724
LweI GCATC 4 cut(s) 40, 213, 553, 715
MaeI CTAG 3 cut(s) 644, 1067, 1199
MaeII ACGT 1 cut(s) 1002
MaeIII GTNAC 6 cut(s) 16, 80, 998, 1003, 1015, 1165
MalI GATC 4 cut(s) 341, 388, 843, 1036
MboI GATC 4 cut(s) 339, 386, 841, 1034
MboII GAAGA 4 cut(s) 48, 105, 531, 1146
MflI RGATCY 1 cut(s) 1034
MhlI GDGCHC 4 cut(s) 15, 157, 729, 815
MluCI AATT 7 cut(s) 103, 159, 601, 664, 697, 817, 1046
Mly113I GGCGCC 1 cut(s) 87
MmeI TCCRAC 6 cut(s) 130, 237, 270, 273, 364, 451
MroXI GAANNNNTTC 2 cut(s) 431, 955
MslI CAYNNNNRTG 1 cut(s) 1191
MspI CCGG 4 cut(s) 78, 444, 450, 822
MspR9I CCNGG 3 cut(s) 445, 451, 823
Mva1269I GAATGC 1 cut(s) 517
MvnI CGCG 3 cut(s) 62, 74, 979
MwoI GCNNNNNNNGC 3 cut(s) 71, 176, 635
NarI GGCGCC 1 cut(s) 87
NciI CCSGG 3 cut(s) 445, 451, 823
NdeI CATATG 1 cut(s) 1172
NdeII GATC 4 cut(s) 339, 386, 841, 1034
NlaIII CATG 5 cut(s) 142, 682, 848, 1054, 1103
NlaIV GGNNCC 3 cut(s) 88, 154, 448
NmeAIII GCCGAG 2 cut(s) 48, 217
NmuCI GTSAC 5 cut(s) 16, 998, 1003, 1015, 1165
NspI RCATGY 1 cut(s) 682
NspV TTCGAA 1 cut(s) 803
PagI TCATGA 2 cut(s) 138, 844
PceI AGGCCT 1 cut(s) 26
PciI ACATGT 1 cut(s) 678
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PctI GAATGC 1 cut(s) 517
PdmI GAANNNNTTC 2 cut(s) 431, 955
PfeI GAWTC 4 cut(s) 185, 242, 530, 805
PflFI GACNNNGTC 1 cut(s) 829
PfoI TCCNGGA 1 cut(s) 821
PkrI GCNGC 4 cut(s) 64, 637, 900, 978
Ple19I CGATCG 1 cut(s) 342
PluTI GGCGCC 1 cut(s) 90
PscI ACATGT 1 cut(s) 678
Psp124BI GAGCTC 2 cut(s) 729, 815
PspN4I GGNNCC 3 cut(s) 88, 154, 448
PspPI GGNCC 2 cut(s) 118, 447
PsuI RGATCY 1 cut(s) 1034
PsyI GACNNNGTC 1 cut(s) 829
PvuI CGATCG 1 cut(s) 342
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
RseI CAYNNNNRTG 1 cut(s) 1191
SacI GAGCTC 2 cut(s) 729, 815
SatI GCNGC 4 cut(s) 63, 636, 899, 977
Sau3AI GATC 4 cut(s) 339, 386, 841, 1034
Sau96I GGNCC 2 cut(s) 118, 447
ScrFI CCNGG 3 cut(s) 445, 451, 823
SduI GDGCHC 4 cut(s) 15, 157, 729, 815
SfaNI GCATC 4 cut(s) 40, 213, 553, 715
SfcI CTRYAG 1 cut(s) 91
SfoI GGCGCC 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 803
SinI GGWCC 2 cut(s) 118, 447
SmiMI CAYNNNNRTG 1 cut(s) 1191
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
Sse9I AATT 7 cut(s) 103, 159, 601, 664, 697, 817, 1046
SseBI AGGCCT 1 cut(s) 26
SsiI CCGC 7 cut(s) 62, 170, 566, 629, 635, 898, 977
SspDI GGCGCC 1 cut(s) 86
SspMI CTAG 3 cut(s) 644, 1067, 1199
SstI GAGCTC 2 cut(s) 729, 815
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 3 cut(s) 443, 449, 821
TaaI ACNGT 4 cut(s) 199, 763, 1030, 1087
TaiI ACGT 1 cut(s) 1005
TaqI TCGA 4 cut(s) 34, 533, 803, 1023
TaqII GACCGA 1 cut(s) 106
TasI AATT 7 cut(s) 103, 159, 601, 664, 697, 817, 1046
TauI GCSGC 4 cut(s) 65, 638, 901, 979
TfiI GAWTC 4 cut(s) 185, 242, 530, 805
TscAI CASTG 3 cut(s) 924, 978, 1068
TseFI GTSAC 5 cut(s) 16, 998, 1003, 1015, 1165
Tsp45I GTSAC 5 cut(s) 16, 998, 1003, 1015, 1165
TspDTI ATGAA 5 cut(s) 127, 155, 864, 1039, 1088
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 3 cut(s) 924, 978, 1068
Tth111I GACNNNGTC 1 cut(s) 829
VpaK11BI GGWCC 2 cut(s) 118, 447
XapI RAATTY 2 cut(s) 817, 1046
XceI RCATGY 1 cut(s) 682
XmiI GTMKAC 2 cut(s) 623, 962
XmnI GAANNNNTTC 2 cut(s) 431, 955
XspI CTAG 3 cut(s) 644, 1067, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.