AT1G10330

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009495
Physical Location & Seq
Forward (+)
3388599 .. 3390150
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10330.1

Sequence Viewer

Length: 1404 bp
ATGCGTTGTACTTCCTTCTCAAGTTTCAGTTTGTCTCTTGAAGACGCACTTCATCTTCTCCAACGTTTCTTATACTCCTCAAACCAGATTAAGCAGATTCACACAGTTCTTTTGACCAGCAACGCATTGGTTGCTTCGAGATGGAAGACGAAATGCGTCTACAACACTCTCATTCGATCCTATCTCACTACAGGAGAATACAAGACTTCTCTTGCTCTCTTCACTCATATGCTTGCAAGCCATGTCCAACCAAATAACCTCACTTTCCCTTCTCTGATTAAAGCAGCTTGCTCATCTTTCTCTGTTTCTTATGGCGTTGCCTTACATGGCCAAGCTCTTAAACGGGGTTTTCTATGGGATCCATTTGTTCAGACTTCTTTTGTCCGGTTCTATGGTGAAGTTGGTGATTTGGAGAGCTCGAGGAAGATGTTTGATGATATTTTAAACCCTTGTGTTGTAGCTTGTAACAGTTTGCTTGACGCTTGTGGAAGAAACGGAGAGATGGATTATGCTTTCGAGTATTTTCAGAGAATGCCTGTAACTGATGTGGTCTCTTGGACCACTGTTATCAACGGGTTTAGTAAGAAAGGGTTACACGCCAAAGCTTTAATGGTTTTTGGTGAGATGATACAGAATGAGCGTGCGGTAATAACACCTAACGAGGCTACTTTCGTAAGTGTACTTTCTTCATGTGCTAATTTTGATCAAGGAGGTATTCGACTGGGGAAGCAGATTCATGGATATGTTATGAGTAAAGAGATCATTCTTACTACAACTTTGGGTACTGCTTTGCTTGATATGTATGGAAAAGCCGGTGACTTGGAGATGGCATTGACCATTTTTGATCAAATTCGTGATAAGAAAGTTTGCGCTTGGAATGCGATAATATCAGCTCTTGCCTCCAATGGTAGACCTAAGCAAGCGCTGGAGATGTTTGAGATGATGAAATCAAGCTATGTGCATCCGAATGGGATCACTTTACTTGCAATACTCACGGCTTGTGCTCGATCTAAACTTGTGGATTTAGGTATTCAGTTGTTCTCTTCTATATGTAGCGAGTACAAGATCATTCCAACATCAGAACATTACGGTTGCGTTGTTGATCTCATTGGCAGAGCTGGACTATTGGTTGACGCAGCCAATTTCATTCAAAGTTTGCCCTTCGAGCCTGATGCTTCTGTTCTCGGAGCTCTTTTGGGCGCTTGCAAGATTCATGAGAATACTGAATTAGGAAATACAGTAGGTAAACAGCTTATTGGACTGCAACCTCAACACTGTGGCCAGTATGTGGCATTGTCGACGTTCAATGCTTTGGATAGCAATTGGTCAGAAGCAGAAAAAATGAGGAAAGCCATGATAGAAGCTGGAATTCGGAAAATCCCTGCTTACAGTGTGCTGACTTAA

Protein Analysis

467

Amino Acids

51.43

Weight (kDa)

7.9

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 52 - 97 2.1e-08 PPR repeat family
PPR_2 PF13041 182 - 233 6.8e-09 PPR repeat family
PPR PF01535 184 - 212 9.2e-07 PPR repeat
PPR_2 PF13041 288 - 336 1.1e-09 PPR repeat family
PPR PF01535 291 - 318 7.8e-07 PPR repeat
E_motif PF20431 405 - 464 1.1e-06 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1288
AccI GTMKAC 3 cut(s) 159, 910, 1298
AciI CCGC 1 cut(s) 644
AclI AACGTT 1 cut(s) 64
AclWI GGATC 4 cut(s) 171, 353, 366, 980
AcoI YGGCCR 2 cut(s) 328, 1279
AcsI RAATTY 2 cut(s) 849, 1368
AfaI GTAC 4 cut(s) 10, 681, 784, 1061
AfeI AGCGCT 1 cut(s) 924
AfiI CCNNNNNNNGG 1 cut(s) 1288
AgsI TTSAA 3 cut(s) 41, 1151, 1306
Alw21I GWGCWC 3 cut(s) 419, 1006, 1192
Alw26I GTCTC 2 cut(s) 39, 556
AlwI GGATC 4 cut(s) 171, 353, 366, 980
Ama87I CYCGRG 1 cut(s) 418
Aor51HI AGCGCT 1 cut(s) 924
AoxI GGCC 2 cut(s) 328, 1279
ApeKI GCWGC 2 cut(s) 284, 1136
ApoI RAATTY 2 cut(s) 849, 1368
AspLEI GCGC 3 cut(s) 872, 925, 1202
AspS9I GGNCC 1 cut(s) 558
AsuHPI GGTGA 4 cut(s) 407, 416, 632, 827
AvaI CYCGRG 1 cut(s) 418
AvaII GGWCC 1 cut(s) 558
BalI TGGCCA 2 cut(s) 330, 1281
BamHI GGATCC 1 cut(s) 358
BanII GRGCYC 2 cut(s) 419, 1192
BbsI GAAGAC 2 cut(s) 48, 152
Bbv12I GWGCWC 3 cut(s) 419, 1006, 1192
BbvI GCAGC 2 cut(s) 296, 1148
BccI CCATC 3 cut(s) 135, 496, 820
BceAI ACGGC 1 cut(s) 1011
BcgI CGANNNNNNTGC 2 cut(s) 1154, 1188
BclI TGATCA 2 cut(s) 703, 844
BcoDI GTCTC 2 cut(s) 39, 556
BfmI CTRYAG 1 cut(s) 189
BfoI RGCGCY 2 cut(s) 926, 1203
BisI GCNGC 2 cut(s) 285, 1137
BlsI GCNGC 2 cut(s) 286, 1138
Bme18I GGWCC 1 cut(s) 558
BmeT110I CYCGRG 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 558
BmiI GGNNCC 1 cut(s) 360
BmrI ACTGGG 1 cut(s) 731
BmsI GCATC 2 cut(s) 970, 1162
BmuI ACTGGG 1 cut(s) 731
BpiI GAAGAC 2 cut(s) 48, 152
BpmI CTGGAG 1 cut(s) 947
Bpu10I CCTNAGC 1 cut(s) 915
BpuEI CTTGAG 1 cut(s) 4
BsaI GGTCTC 1 cut(s) 556
BsaWI WCCGGW 1 cut(s) 384
Bsc4I CCNNNNNNNGG 1 cut(s) 1288
Bse118I RCCGGY 1 cut(s) 812
Bse1I ACTGG 2 cut(s) 726, 1282
BseGI GGATG 1 cut(s) 961
BseLI CCNNNNNNNGG 1 cut(s) 1288
BseNI ACTGG 2 cut(s) 726, 1282
BseRI GAGGAG 1 cut(s) 67
BseXI GCAGC 2 cut(s) 296, 1148
BshFI GGCC 2 cut(s) 330, 1281
BsiHKAI GWGCWC 3 cut(s) 419, 1006, 1192
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 2 cut(s) 385, 813
BslI CCNNNNNNNGG 1 cut(s) 1288
BsmAI GTCTC 2 cut(s) 39, 556
BsmI GAATGC 2 cut(s) 537, 883
BsnI GGCC 2 cut(s) 330, 1281
Bso31I GGTCTC 1 cut(s) 556
BsoBI CYCGRG 1 cut(s) 418
Bsp1286I GDGCHC 3 cut(s) 419, 1006, 1192
Bsp143I GATC 9 cut(s) 176, 358, 703, 759, 844, 972, 1007, 1065, 1102
BspACI CCGC 1 cut(s) 644
BspANI GGCC 2 cut(s) 330, 1281
BspHI TCATGA 1 cut(s) 1213
BspLI GGNNCC 1 cut(s) 360
BspPI GGATC 4 cut(s) 171, 353, 366, 980
BspTNI GGTCTC 1 cut(s) 556
BsrFI RCCGGY 1 cut(s) 812
BsrI ACTGG 2 cut(s) 726, 1282
BssAI RCCGGY 1 cut(s) 812
BssMI GATC 9 cut(s) 176, 358, 703, 759, 844, 972, 1007, 1065, 1102
Bst4CI ACNGT 7 cut(s) 106, 470, 565, 1091, 1240, 1277, 1391
Bst6I CTCTTC 2 cut(s) 224, 1048
BstAPI GCANNNNNTGC 1 cut(s) 131
BstC8I GCNNGC 6 cut(s) 234, 238, 289, 642, 921, 1204
BstDEI CTNAG 1 cut(s) 915
BstF5I GGATG 1 cut(s) 961
BstH2I RGCGCY 2 cut(s) 926, 1203
BstHHI GCGC 3 cut(s) 872, 925, 1202
BstKTI GATC 9 cut(s) 179, 361, 706, 762, 847, 975, 1010, 1068, 1105
BstMAI GTCTC 2 cut(s) 39, 556
BstMBI GATC 9 cut(s) 176, 358, 703, 759, 844, 972, 1007, 1065, 1102
BstMWI GCNNNNNNNGC 3 cut(s) 131, 878, 1165
BstSFI CTRYAG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 296, 1148
BstV2I GAAGAC 2 cut(s) 48, 152
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 2 cut(s) 330, 1281
BtsCI GGATG 1 cut(s) 961
BtsIMutI CAGTG 3 cut(s) 561, 1273, 1396
Cac8I GCNNGC 6 cut(s) 234, 238, 289, 642, 921, 1204
CciI TCATGA 1 cut(s) 1213
CfoI GCGC 3 cut(s) 872, 925, 1202
Cfr10I RCCGGY 1 cut(s) 812
Cfr13I GGNCC 1 cut(s) 558
CseI GACGC 4 cut(s) 53, 145, 488, 1142
Csp6I GTAC 4 cut(s) 9, 680, 783, 1060
CviAII CATG 6 cut(s) 242, 326, 690, 737, 1214, 1354
CviQI GTAC 4 cut(s) 9, 680, 783, 1060
DdeI CTNAG 1 cut(s) 915
DpnI GATC 9 cut(s) 178, 360, 705, 761, 846, 974, 1009, 1067, 1104
DpnII GATC 9 cut(s) 176, 358, 703, 759, 844, 972, 1007, 1065, 1102
DraI TTTAAA 1 cut(s) 444
EaeI YGGCCR 2 cut(s) 328, 1279
Eam1104I CTCTTC 2 cut(s) 224, 1048
EarI CTCTTC 2 cut(s) 224, 1048
Ecl136II GAGCTC 2 cut(s) 417, 1190
Eco24I GRGCYC 2 cut(s) 419, 1192
Eco31I GGTCTC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 558
Eco47III AGCGCT 1 cut(s) 924
Eco53kI GAGCTC 2 cut(s) 417, 1190
Eco88I CYCGRG 1 cut(s) 418
EcoICRI GAGCTC 2 cut(s) 417, 1190
EcoRI GAATTC 1 cut(s) 1368
EcoT38I GRGCYC 2 cut(s) 419, 1192
FaeI CATG 6 cut(s) 245, 329, 693, 740, 1217, 1357
FalI AAGNNNNNCTT 2 cut(s) 33, 65
FatI CATG 6 cut(s) 241, 325, 689, 736, 1213, 1353
FauNDI CATATG 1 cut(s) 228
FbaI TGATCA 2 cut(s) 703, 844
FblI GTMKAC 3 cut(s) 159, 910, 1298
Fnu4HI GCNGC 2 cut(s) 285, 1137
FokI GGATG 1 cut(s) 948
FriOI GRGCYC 2 cut(s) 419, 1192
Fsp4HI GCNGC 2 cut(s) 285, 1137
GlaI GCGC 3 cut(s) 871, 924, 1201
GluI GCNGC 2 cut(s) 285, 1137
GsuI CTGGAG 1 cut(s) 947
HaeII RGCGCY 2 cut(s) 926, 1203
HaeIII GGCC 2 cut(s) 330, 1281
HapII CCGG 2 cut(s) 385, 813
HgaI GACGC 4 cut(s) 53, 145, 488, 1142
HhaI GCGC 3 cut(s) 872, 925, 1202
Hin1II CATG 6 cut(s) 245, 329, 693, 740, 1217, 1357
Hin6I GCGC 3 cut(s) 870, 923, 1200
HinP1I GCGC 3 cut(s) 870, 923, 1200
HincII GTYRAC 2 cut(s) 1132, 1299
HindII GTYRAC 2 cut(s) 1132, 1299
HindIII AAGCTT 1 cut(s) 603
HinfI GANTC 3 cut(s) 97, 733, 1210
HpaII CCGG 2 cut(s) 385, 813
HphI GGTGA 4 cut(s) 407, 416, 632, 827
Hpy166II GTNNAC 6 cut(s) 160, 680, 911, 1132, 1247, 1299
Hpy188I TCNGA 8 cut(s) 276, 372, 528, 966, 1081, 1187, 1330, 1374
Hpy188III TCNNGA 4 cut(s) 38, 138, 854, 1214
Hpy8I GTNNAC 6 cut(s) 160, 680, 911, 1132, 1247, 1299
Hpy99I CGWCG 1 cut(s) 1303
HpyAV CCTTC 3 cut(s) 25, 279, 1171
HpyCH4III ACNGT 7 cut(s) 106, 470, 565, 1091, 1240, 1277, 1391
HpyCH4IV ACGT 2 cut(s) 64, 1301
HpyCH4V TGCA 5 cut(s) 236, 961, 986, 1206, 1264
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 878, 1165
HpyF3I CTNAG 1 cut(s) 915
HpySE526I ACGT 2 cut(s) 64, 1301
Hsp92II CATG 6 cut(s) 245, 329, 693, 740, 1217, 1357
HspAI GCGC 3 cut(s) 870, 923, 1200
Ksp22I TGATCA 2 cut(s) 703, 844
Kzo9I GATC 9 cut(s) 176, 358, 703, 759, 844, 972, 1007, 1065, 1102
LmnI GCTCC 1 cut(s) 1187
Lsp1109I GCAGC 2 cut(s) 296, 1148
LweI GCATC 2 cut(s) 970, 1162
MaeII ACGT 2 cut(s) 64, 1301
MaeIII GTNAC 4 cut(s) 464, 538, 591, 815
MalI GATC 9 cut(s) 178, 360, 705, 761, 846, 974, 1009, 1067, 1104
MboI GATC 9 cut(s) 176, 358, 703, 759, 844, 972, 1007, 1065, 1102
MboII GAAGA 8 cut(s) 47, 53, 157, 211, 436, 501, 678, 1035
MfeI CAATTG 1 cut(s) 1321
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 3 cut(s) 419, 1006, 1192
MlsI TGGCCA 2 cut(s) 330, 1281
MluCI AATT 6 cut(s) 697, 849, 1141, 1226, 1321, 1368
MluNI TGGCCA 2 cut(s) 330, 1281
MmeI TCCRAC 3 cut(s) 85, 271, 1097
MnlI CCTC 8 cut(s) 88, 269, 414, 655, 704, 910, 1278, 1338
Mox20I TGGCCA 2 cut(s) 330, 1281
MscI TGGCCA 2 cut(s) 330, 1281
MseI TTAA 6 cut(s) 90, 279, 339, 443, 608, 1402
MslI CAYNNNNRTG 3 cut(s) 227, 741, 966
Msp20I TGGCCA 2 cut(s) 330, 1281
MspI CCGG 2 cut(s) 385, 813
MunI CAATTG 1 cut(s) 1321
Mva1269I GAATGC 2 cut(s) 537, 883
MwoI GCNNNNNNNGC 3 cut(s) 131, 878, 1165
NdeI CATATG 1 cut(s) 228
NdeII GATC 9 cut(s) 176, 358, 703, 759, 844, 972, 1007, 1065, 1102
NlaIII CATG 6 cut(s) 245, 329, 693, 740, 1217, 1357
NlaIV GGNNCC 1 cut(s) 360
NmuCI GTSAC 1 cut(s) 815
PaeR7I CTCGAG 1 cut(s) 418
PagI TCATGA 1 cut(s) 1213
PctI GAATGC 2 cut(s) 537, 883
PfeI GAWTC 3 cut(s) 97, 733, 1210
PflMI CCANNNNNTGG 1 cut(s) 1288
PkrI GCNGC 2 cut(s) 286, 1138
Psp124BI GAGCTC 2 cut(s) 419, 1192
Psp1406I AACGTT 1 cut(s) 64
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 1 cut(s) 558
PspXI VCTCGAGB 1 cut(s) 418
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 4 cut(s) 10, 681, 784, 1061
RsaNI GTAC 4 cut(s) 9, 680, 783, 1060
RseI CAYNNNNRTG 3 cut(s) 227, 741, 966
SacI GAGCTC 2 cut(s) 419, 1192
SalI GTCGAC 1 cut(s) 1297
SaqAI TTAA 6 cut(s) 90, 279, 339, 443, 608, 1402
SatI GCNGC 2 cut(s) 285, 1137
Sau3AI GATC 9 cut(s) 176, 358, 703, 759, 844, 972, 1007, 1065, 1102
Sau96I GGNCC 1 cut(s) 558
SduI GDGCHC 3 cut(s) 419, 1006, 1192
SfaNI GCATC 2 cut(s) 970, 1162
SfcI CTRYAG 1 cut(s) 189
Sfr274I CTCGAG 1 cut(s) 418
SinI GGWCC 1 cut(s) 558
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 3 cut(s) 227, 741, 966
SmlI CTYRAG 2 cut(s) 19, 418
SmoI CTYRAG 2 cut(s) 19, 418
Sse9I AATT 6 cut(s) 697, 849, 1141, 1226, 1321, 1368
SsiI CCGC 1 cut(s) 644
SstI GAGCTC 2 cut(s) 419, 1192
TaaI ACNGT 7 cut(s) 106, 470, 565, 1091, 1240, 1277, 1391
TaiI ACGT 2 cut(s) 67, 1304
TaqI TCGA 8 cut(s) 137, 175, 419, 516, 718, 1006, 1164, 1298
TasI AATT 6 cut(s) 697, 849, 1141, 1226, 1321, 1368
TatI WGTACW 3 cut(s) 8, 679, 1059
TfiI GAWTC 3 cut(s) 97, 733, 1210
Tru1I TTAA 6 cut(s) 90, 279, 339, 443, 608, 1402
Tru9I TTAA 6 cut(s) 90, 279, 339, 443, 608, 1402
TscAI CASTG 3 cut(s) 568, 1280, 1396
TseFI GTSAC 1 cut(s) 815
TseI GCWGC 2 cut(s) 284, 1136
Tsp45I GTSAC 1 cut(s) 815
TspDTI ATGAA 6 cut(s) 41, 678, 725, 959, 1135, 1202
TspGWI ACGGA 1 cut(s) 510
TspRI CASTG 3 cut(s) 568, 1280, 1396
Van91I CCANNNNNTGG 1 cut(s) 1288
VpaK11BI GGWCC 1 cut(s) 558
XapI RAATTY 2 cut(s) 849, 1368
XcmI CCANNNNNNNNNTGG 2 cut(s) 124, 607
XhoI CTCGAG 1 cut(s) 418
XmiI GTMKAC 3 cut(s) 159, 910, 1298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.