pycom17g09460

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009495
Physical Location & Seq
Forward (+)
7216879 .. 7217619
741 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g09460.1

Sequence Viewer

Length: 741 bp
ATGATTAATGGGTTTGGGAAGAATGGGGATATGGTTTCCGCTAATATTCTCTTTGAAAGTATGCCGGCGAGGGACGTGGTTTCGTGGACAAGCGTTATCAACGGGTTCAGGAGAAATGGGTATTTTACTGAGGGAATTTGGTTTTTTAAGATGATGGTGAATTATGAAGATGAGATTGGTTGTTTTGTGAAGCCGAATGAAGCTACTTATGTTAGGGTTTTTTCTTTGTGTGCTAATTTAGATGGGTCGGGATCTATTTATTGGGGAAAGCAAATACATGGGCATGTTATTAGGAATACCATCGAATTGACTGCGTTTTTGGGGACTGTGTTGGTGGATCTTTACGGGAAGACTCGTTGTTTGAAAAGCACTGGATACCTGTTCAAGAAAATGGTAGTTAAAGAAGTTTGTACTTGGAATTCAATGATTTCAGCACTTTCTTTGAATGGTAGGGAGAGGGAGGATTTGGACTTGTTTGAAAAAATGAAGATGGAAAACTTGCAGCCTAATGAGGTCACATTTGTCACAGTTCTCACGGCTTGTGCTCGTGGAAAACCGGTGAAGTTTGGTTTGGAATTGTTTAGATCAATGTCCAATGATTTCGGGGTTGAGCCGATAATGGAACACCACGGGTGTGTGGTTGACCTCTTGGGCAGAGCAGGACTGTTTTGGGAGGCAACTGAAGTTATTAAGAGCATGCCCCGATGCCTCTGTGTTGGGAGCTATTTTAGGTTCTTGTAA

Protein Analysis

247

Amino Acids

27.89

Weight (kDa)

7.54

Isoelectric Point (pI)

29.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 136 - 183 1.2e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 39
AclWI GGATC 2 cut(s) 259, 345
AcsI RAATTY 2 cut(s) 135, 418
AcuI CTGAAG 1 cut(s) 702
AfaI GTAC 1 cut(s) 412
AgeI ACCGGT 1 cut(s) 556
AgsI TTSAA 6 cut(s) 56, 364, 385, 423, 445, 479
AjiI CACGTC 1 cut(s) 76
AjuI GAANNNNNNNTTGG 4 cut(s) 159, 191, 554, 586
AleI CACNNNNGTG 1 cut(s) 633
AluBI AGCT 2 cut(s) 203, 723
AluI AGCT 2 cut(s) 203, 723
Alw21I GWGCWC 1 cut(s) 547
AlwI GGATC 2 cut(s) 259, 345
ApeKI GCWGC 1 cut(s) 502
ApoI RAATTY 2 cut(s) 135, 418
ArsI GACNNNNNNTTYG 4 cut(s) 301, 333, 343, 375
AseI ATTAAT 1 cut(s) 6
AsiGI ACCGGT 1 cut(s) 556
AsuHPI GGTGA 2 cut(s) 169, 571
BauI CACGAG 1 cut(s) 546
BbsI GAAGAC 1 cut(s) 356
Bbv12I GWGCWC 1 cut(s) 547
BbvI GCAGC 1 cut(s) 514
BccI CCATC 4 cut(s) 148, 236, 308, 484
BceAI ACGGC 1 cut(s) 552
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciVI GTATCC 1 cut(s) 368
BfuI GTATCC 1 cut(s) 368
BisI GCNGC 1 cut(s) 503
BlsI GCNGC 1 cut(s) 504
BmgBI CACGTC 1 cut(s) 76
BmsI GCATC 1 cut(s) 695
BpiI GAAGAC 1 cut(s) 356
BsaJI CCNNGG 1 cut(s) 628
BsaWI WCCGGW 1 cut(s) 556
Bse118I RCCGGY 2 cut(s) 64, 556
Bse1I ACTGG 1 cut(s) 376
BseDI CCNNGG 1 cut(s) 628
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 1 cut(s) 376
BseXI GCAGC 1 cut(s) 514
BshTI ACCGGT 1 cut(s) 556
BsiHKAI GWGCWC 1 cut(s) 547
BsiSI CCGG 2 cut(s) 65, 557
BslFI GGGAC 2 cut(s) 86, 337
BsmFI GGGAC 2 cut(s) 86, 337
Bsp1286I GDGCHC 1 cut(s) 547
Bsp143I GATC 3 cut(s) 251, 337, 584
BspACI CCGC 1 cut(s) 39
BspCNI CTCAG 1 cut(s) 121
BspPI GGATC 2 cut(s) 259, 345
BsrFI RCCGGY 2 cut(s) 64, 556
BsrI ACTGG 1 cut(s) 376
BssAI RCCGGY 2 cut(s) 64, 556
BssECI CCNNGG 1 cut(s) 628
BssMI GATC 3 cut(s) 251, 337, 584
BssSI CACGAG 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 546
Bst4CI ACNGT 3 cut(s) 328, 529, 666
BstC8I GCNNGC 2 cut(s) 66, 698
BstDEI CTNAG 1 cut(s) 129
BstDSI CCRYGG 1 cut(s) 628
BstKTI GATC 3 cut(s) 254, 340, 587
BstMBI GATC 3 cut(s) 251, 337, 584
BstNSI RCATGY 2 cut(s) 287, 700
BstV1I GCAGC 1 cut(s) 514
BstV2I GAAGAC 1 cut(s) 356
BstX2I RGATCY 2 cut(s) 251, 337
BstYI RGATCY 2 cut(s) 251, 337
BsuI GTATCC 1 cut(s) 368
BtgI CCRYGG 1 cut(s) 628
BtrI CACGTC 1 cut(s) 76
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 2 cut(s) 66, 698
Cfr10I RCCGGY 2 cut(s) 64, 556
Csp6I GTAC 1 cut(s) 411
CspAI ACCGGT 1 cut(s) 556
CviAII CATG 3 cut(s) 278, 284, 697
CviJI RGCY 6 cut(s) 193, 203, 505, 539, 613, 723
CviKI_1 RGCY 6 cut(s) 193, 203, 505, 539, 613, 723
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 1 cut(s) 129
DpnI GATC 3 cut(s) 253, 339, 586
DpnII GATC 3 cut(s) 251, 337, 584
Eco57I CTGAAG 1 cut(s) 702
EcoRI GAATTC 1 cut(s) 418
FaeI CATG 3 cut(s) 281, 287, 700
FaiI YATR 7 cut(s) 32, 62, 165, 210, 279, 285, 698
FaqI GGGAC 2 cut(s) 86, 337
FatI CATG 3 cut(s) 277, 283, 696
Fnu4HI GCNGC 1 cut(s) 503
Fsp4HI GCNGC 1 cut(s) 503
GluI GCNGC 1 cut(s) 503
HapII CCGG 2 cut(s) 65, 557
Hin1II CATG 3 cut(s) 281, 287, 700
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HinfI GANTC 1 cut(s) 352
HpaII CCGG 2 cut(s) 65, 557
HphI GGTGA 2 cut(s) 169, 571
Hpy166II GTNNAC 2 cut(s) 87, 643
Hpy188III TCNNGA 3 cut(s) 109, 249, 385
Hpy8I GTNNAC 2 cut(s) 87, 643
HpyCH4III ACNGT 3 cut(s) 328, 529, 666
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 1 cut(s) 502
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 3 cut(s) 281, 287, 700
KroI GCCGGC 1 cut(s) 64
KroNI GCCGGC 1 cut(s) 66
Kzo9I GATC 3 cut(s) 251, 337, 584
LmnI GCTCC 1 cut(s) 720
LpnPI CCDG 6 cut(s) 78, 94, 357, 392, 570, 645
Lsp1109I GCAGC 1 cut(s) 514
LweI GCATC 1 cut(s) 695
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 2 cut(s) 514, 523
MalI GATC 3 cut(s) 253, 339, 586
MboI GATC 3 cut(s) 251, 337, 584
MboII GAAGA 4 cut(s) 31, 179, 361, 499
MflI RGATCY 2 cut(s) 251, 337
MhlI GDGCHC 1 cut(s) 547
MluCI AATT 6 cut(s) 135, 160, 235, 305, 418, 575
MlyI GAGTC 1 cut(s) 346
MnlI CCTC 8 cut(s) 63, 124, 450, 454, 505, 656, 667, 719
MroNI GCCGGC 1 cut(s) 64
MseI TTAA 4 cut(s) 6, 147, 399, 690
MslI CAYNNNNRTG 2 cut(s) 282, 633
MspI CCGG 2 cut(s) 65, 557
NaeI GCCGGC 1 cut(s) 66
NdeII GATC 3 cut(s) 251, 337, 584
NgoMIV GCCGGC 1 cut(s) 64
NlaIII CATG 3 cut(s) 281, 287, 700
NmuCI GTSAC 2 cut(s) 514, 523
NspI RCATGY 2 cut(s) 287, 700
OliI CACNNNNGTG 1 cut(s) 633
PaeI GCATGC 1 cut(s) 700
PdiI GCCGGC 1 cut(s) 66
PinAI ACCGGT 1 cut(s) 556
PkrI GCNGC 1 cut(s) 504
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PshBI ATTAAT 1 cut(s) 6
PsuI RGATCY 2 cut(s) 251, 337
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 2 cut(s) 282, 633
SaqAI TTAA 4 cut(s) 6, 147, 399, 690
SatI GCNGC 1 cut(s) 503
Sau3AI GATC 3 cut(s) 251, 337, 584
SchI GAGTC 1 cut(s) 346
SduI GDGCHC 1 cut(s) 547
SetI ASST 7 cut(s) 78, 205, 381, 516, 648, 725, 734
SfaNI GCATC 1 cut(s) 695
SmiMI CAYNNNNRTG 2 cut(s) 282, 633
SphI GCATGC 1 cut(s) 700
Sse9I AATT 6 cut(s) 135, 160, 235, 305, 418, 575
SsiI CCGC 1 cut(s) 39
SspI AATATT 1 cut(s) 46
TaaI ACNGT 3 cut(s) 328, 529, 666
TaiI ACGT 1 cut(s) 78
TaqI TCGA 1 cut(s) 303
TasI AATT 6 cut(s) 135, 160, 235, 305, 418, 575
TatI WGTACW 1 cut(s) 410
Tru1I TTAA 4 cut(s) 6, 147, 399, 690
Tru9I TTAA 4 cut(s) 6, 147, 399, 690
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 2 cut(s) 514, 523
TseI GCWGC 1 cut(s) 502
Tsp45I GTSAC 2 cut(s) 514, 523
TspDTI ATGAA 3 cut(s) 180, 213, 500
TspRI CASTG 1 cut(s) 376
VspI ATTAAT 1 cut(s) 6
XapI RAATTY 2 cut(s) 135, 418
XceI RCATGY 2 cut(s) 287, 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.