Rw3G001550

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0009495
Physical Location & Seq
Forward (+)
1295812 .. 1297230
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G001550.1

Sequence Viewer

Length: 1419 bp
ATGCTGGAGTATTTGCTTCAGCTCCTCCAACGCTTCTTGAAAAGCCCAAACCAAATCAGACAAATCCATTCCCGCCTAATCACCAACGGTCATCTCCGTCGTAACCCAAACCCTAAATTTTCAAAATGGAGGACAACCCTATTCTACAACACTCTGATCAGAGCCCACCTCGGTTTCGGCGAAGCCCACAAGACTTTTTTTCTCTTTACCCAGATGCTTTCCCACCAAGCTCACCCCAACAGCCACACATTCCCTCCGCTCGTCAAAGCCGAAGCCGTCGCCGGCGGCGGTGGCTGGCCGTGTAATGGCACGGCTCTCCACGCCCAAGTTGCGAAACGCGGCGTTTGGGGTGATCCGGTTGTGCAGACGTCGTTTGTGAGCATGTATGCTCGGTTTGGTGAAGTGCGGGATGCACGTAAGGTGTTCGATGAAATGACTGAACCGTGTGTGGTTGCGTGCAATGTGATGATTGATGGGTTGGGGAAGAATGGGGACATGAGTGGTGCTGTTTCAATGTTTGAAAGGATGCCGGAGAGGGATGTGGTTTCGTGGACGAGTGTGATTAGTGGGTTTGGGAGGAATGGATGGTTTGGTGAGGGGGTTTGGTTTTTCAAGAGGATGATGGGGAGTGAGGATGTGAAGTGTGGTTTTGTGAAGCCAAATGAAGCTACATTTGTTAGTGTGCTTTCTTCATGTGCTAATTTGGATGGGTGGGGATCTCTTTATTGGGGGAAGCAAATTCATGGGTATGTTGTTAGGAATGAGGTGCAGTTGAGTGTTTTTATGGGGACTGCATTGGTGGATCTTTATGGGAAGTCGGGTTCTTTGAGTGGTGCTTGGAATGTTTTTGAGGAAATGAGGGTTAAAGAAGTTTGTACATGGAACGCAATGATCTCAGCGCTTTCTTTGAATGGCAGGGAGAATGAGGCTTTGGAGTTGTTTGAGAAAATGAAGATGGAAAAGAGGTTGCGGCCTAATGAGGTTACATTTGTTGCAGTTCTCACAGCTTGTGCTCGTGGGAATTCGGTGAAGTGTGGTTTGGATTTGTTTAAATCAATGTCCAAAAACTTTGGGGTTGAACCAATAATGGAACACTATGGTTGTGTGGTTGATCTCCTAGGCAGAGCAGGACTTTTTAAGGAGGCAACTGAACTTCTTAAGAACATGCCTTTTGAGCCTGATGCTTCTGTGTTGGGGGCTCTTTTGGGTTCTTGTAAGATTCATGGGACCACTGAGCTGGGAAATGAAGTTGGGAAAAAATTGATTGAGTTGCAGCCACAACATTGTGGACGATACTTGGTTTTGTCAAACATTTATGCTGGGAACGAGAGGTGGGATCATGCTGCTGCTGTGAGGAAAGCAATGGTACATGCTGGACTTAAGAAAATTCCAGCCTTTAGTATGATTGACGTAATGTAG

Protein Analysis

472

Amino Acids

52.58

Weight (kDa)

8.93

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 148 - 180 9e-07 PPR repeat family
PPR PF01535 151 - 180 2e-07 PPR repeat
PPR PF01535 182 - 209 6.1e-06 PPR repeat
PPR_2 PF13041 291 - 339 4.2e-10 PPR repeat family
PPR_3 PF13812 291 - 337 4.9e-06 Pentatricopeptide repeat domain
PPR PF01535 293 - 319 1.6e-07 PPR repeat
E_motif PF20431 409 - 469 1.8e-11 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 371
AccBSI CCGCTC 1 cut(s) 259
AccII CGCG 1 cut(s) 339
AciI CCGC 7 cut(s) 73, 257, 285, 288, 339, 406, 970
AclWI GGATC 4 cut(s) 347, 724, 810, 1344
AcoI YGGCCR 1 cut(s) 296
AcsI RAATTY 4 cut(s) 116, 738, 1021, 1386
AcyI GRCGYC 1 cut(s) 368
AfaI GTAC 2 cut(s) 877, 1368
AfeI AGCGCT 1 cut(s) 900
AfiI CCNNNNNNNGG 1 cut(s) 305
AflII CTTAAG 2 cut(s) 1157, 1379
AgsI TTSAA 7 cut(s) 40, 123, 513, 521, 613, 910, 1079
AjuI GAANNNNNNNTTGG 4 cut(s) 914, 946, 1022, 1054
AloI GAACNNNNNNTCC 2 cut(s) 805, 837
AluBI AGCT 5 cut(s) 22, 230, 668, 1007, 1237
AluI AGCT 5 cut(s) 22, 230, 668, 1007, 1237
Alw21I GWGCWC 1 cut(s) 1015
AlwI GGATC 4 cut(s) 347, 724, 810, 1344
Aor51HI AGCGCT 1 cut(s) 900
AoxI GGCC 2 cut(s) 296, 971
ApeKI GCWGC 3 cut(s) 1273, 1343, 1346
ApoI RAATTY 4 cut(s) 116, 738, 1021, 1386
AspA2I CCTAGG 1 cut(s) 1117
AspLEI GCGC 1 cut(s) 901
AspS9I GGNCC 1 cut(s) 1227
AsuHPI GGTGA 6 cut(s) 73, 224, 362, 410, 605, 1039
AvaII GGWCC 1 cut(s) 1227
AvrII CCTAGG 1 cut(s) 1117
BanII GRGCYC 2 cut(s) 166, 1201
BauI CACGAG 1 cut(s) 1014
Bbv12I GWGCWC 1 cut(s) 1015
BbvI GCAGC 3 cut(s) 1285, 1330, 1333
BccI CCATC 5 cut(s) 467, 579, 616, 701, 949
BceAI ACGGC 3 cut(s) 260, 283, 327
BclI TGATCA 1 cut(s) 156
BfaI CTAG 1 cut(s) 1118
BfoI RGCGCY 1 cut(s) 902
BfrI CTTAAG 2 cut(s) 1157, 1379
BisI GCNGC 6 cut(s) 286, 340, 971, 1274, 1344, 1347
BlnI CCTAGG 1 cut(s) 1117
BlsI GCNGC 6 cut(s) 287, 341, 972, 1275, 1345, 1348
Bme18I GGWCC 1 cut(s) 1227
BmgT120I GGNCC 1 cut(s) 1227
BmiI GGNNCC 1 cut(s) 1228
BmsI GCATC 4 cut(s) 204, 400, 516, 1171
BplI GAGNNNNNCTC 2 cut(s) 153, 185
BpmI CTGGAG 1 cut(s) 26
BsaAI YACGTR 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 368
BsaJI CCNNGG 2 cut(s) 169, 1117
BsaWI WCCGGW 1 cut(s) 355
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse118I RCCGGY 1 cut(s) 281
Bse3DI GCAATG 3 cut(s) 466, 894, 1368
BseDI CCNNGG 2 cut(s) 169, 1117
BseGI GGATG 7 cut(s) 415, 531, 544, 590, 624, 640, 712
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMI GCAATG 3 cut(s) 466, 894, 1368
BseMII CTCAG 2 cut(s) 909, 1224
BseRI GAGGAG 1 cut(s) 14
BseXI GCAGC 3 cut(s) 1285, 1330, 1333
BseYI CCCAGC 2 cut(s) 1237, 1319
BsgI GTGCAG 2 cut(s) 383, 788
Bsh1236I CGCG 1 cut(s) 339
BshFI GGCC 2 cut(s) 298, 973
BsiHKAI GWGCWC 1 cut(s) 1015
BsiSI CCGG 3 cut(s) 282, 356, 530
BslFI GGGAC 3 cut(s) 506, 802, 1240
BslI CCNNNNNNNGG 1 cut(s) 305
BsmFI GGGAC 3 cut(s) 506, 802, 1240
BsnI GGCC 2 cut(s) 298, 973
Bsp1286I GDGCHC 3 cut(s) 166, 1015, 1201
Bsp1407I TGTACA 1 cut(s) 875
Bsp143I GATC 7 cut(s) 156, 352, 716, 802, 891, 1111, 1336
BspACI CCGC 7 cut(s) 73, 257, 285, 288, 339, 406, 970
BspANI GGCC 2 cut(s) 298, 973
BspCNI CTCAG 2 cut(s) 908, 1225
BspFNI CGCG 1 cut(s) 339
BspLI GGNNCC 1 cut(s) 1228
BspPI GGATC 4 cut(s) 347, 724, 810, 1344
BspTI CTTAAG 2 cut(s) 1157, 1379
BsrBI CCGCTC 1 cut(s) 259
BsrDI GCAATG 3 cut(s) 466, 894, 1368
BsrFI RCCGGY 1 cut(s) 281
BsrGI TGTACA 1 cut(s) 875
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 2 cut(s) 169, 1117
BssMI GATC 7 cut(s) 156, 352, 716, 802, 891, 1111, 1336
BssNI GRCGYC 1 cut(s) 368
BssSI CACGAG 1 cut(s) 1014
BssT1I CCWWGG 1 cut(s) 1117
Bst2BI CACGAG 1 cut(s) 1014
Bst4CI ACNGT 2 cut(s) 89, 444
BstACI GRCGYC 1 cut(s) 368
BstAFI CTTAAG 2 cut(s) 1157, 1379
BstAUI TGTACA 1 cut(s) 875
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 3 cut(s) 283, 296, 457
BstDEI CTNAG 2 cut(s) 895, 1233
BstF5I GGATG 7 cut(s) 415, 531, 544, 590, 624, 640, 712
BstFNI CGCG 1 cut(s) 339
BstH2I RGCGCY 1 cut(s) 902
BstHHI GCGC 1 cut(s) 901
BstKTI GATC 7 cut(s) 159, 355, 719, 805, 894, 1114, 1339
BstMBI GATC 7 cut(s) 156, 352, 716, 802, 891, 1111, 1336
BstMWI GCNNNNNNNGC 4 cut(s) 291, 320, 329, 1174
BstNSI RCATGY 3 cut(s) 385, 1168, 1373
BstUI CGCG 1 cut(s) 339
BstV1I GCAGC 3 cut(s) 1285, 1330, 1333
BstX2I RGATCY 2 cut(s) 716, 802
BstXI CCANNNNNNTGG 1 cut(s) 1237
BstYI RGATCY 2 cut(s) 716, 802
BsuRI GGCC 2 cut(s) 298, 973
BtsCI GGATG 7 cut(s) 415, 531, 544, 590, 624, 640, 712
BtsIMutI CAGTG 1 cut(s) 1230
Cac8I GCNNGC 3 cut(s) 283, 296, 457
CfoI GCGC 1 cut(s) 901
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 1 cut(s) 1227
Csp6I GTAC 2 cut(s) 876, 1367
CviAII CATG 9 cut(s) 382, 496, 693, 743, 879, 1165, 1223, 1340, 1370
CviQI GTAC 2 cut(s) 876, 1367
DdeI CTNAG 2 cut(s) 895, 1233
DpnI GATC 7 cut(s) 158, 354, 718, 804, 893, 1113, 1338
DpnII GATC 7 cut(s) 156, 352, 716, 802, 891, 1111, 1336
DraI TTTAAA 1 cut(s) 1051
EaeI YGGCCR 1 cut(s) 296
Eco130I CCWWGG 1 cut(s) 1117
Eco24I GRGCYC 2 cut(s) 166, 1201
Eco47I GGWCC 1 cut(s) 1227
Eco47III AGCGCT 1 cut(s) 900
EcoRI GAATTC 1 cut(s) 1021
EcoT14I CCWWGG 1 cut(s) 1117
EcoT38I GRGCYC 2 cut(s) 166, 1201
ErhI CCWWGG 1 cut(s) 1117
FaeI CATG 9 cut(s) 385, 499, 696, 746, 882, 1168, 1226, 1343, 1373
FaqI GGGAC 3 cut(s) 506, 802, 1240
FatI CATG 9 cut(s) 381, 495, 692, 742, 878, 1164, 1222, 1339, 1369
FauI CCCGC 2 cut(s) 80, 399
FbaI TGATCA 1 cut(s) 156
Fnu4HI GCNGC 6 cut(s) 286, 340, 971, 1274, 1344, 1347
FokI GGATG 7 cut(s) 422, 538, 551, 597, 631, 647, 719
FriOI GRGCYC 2 cut(s) 166, 1201
Fsp4HI GCNGC 6 cut(s) 286, 340, 971, 1274, 1344, 1347
FspBI CTAG 1 cut(s) 1118
GlaI GCGC 1 cut(s) 900
GluI GCNGC 6 cut(s) 286, 340, 971, 1274, 1344, 1347
GsaI CCCAGC 2 cut(s) 1241, 1323
GsuI CTGGAG 1 cut(s) 26
HaeII RGCGCY 1 cut(s) 902
HaeIII GGCC 2 cut(s) 298, 973
HapII CCGG 3 cut(s) 282, 356, 530
HhaI GCGC 1 cut(s) 901
Hin1I GRCGYC 1 cut(s) 368
Hin1II CATG 9 cut(s) 385, 499, 696, 746, 882, 1168, 1226, 1343, 1373
Hin6I GCGC 1 cut(s) 899
HinP1I GCGC 1 cut(s) 899
HinfI GANTC 1 cut(s) 1219
HpaII CCGG 3 cut(s) 282, 356, 530
HphI GGTGA 6 cut(s) 73, 224, 362, 410, 605, 1039
Hpy166II GTNNAC 2 cut(s) 552, 1289
Hpy188I TCNGA 3 cut(s) 59, 156, 161
Hpy188III TCNNGA 2 cut(s) 37, 613
Hpy8I GTNNAC 2 cut(s) 552, 1289
Hpy99I CGWCG 3 cut(s) 102, 281, 373
HpyCH4III ACNGT 2 cut(s) 89, 444
HpyCH4IV ACGT 3 cut(s) 368, 415, 1410
HpyCH4V TGCA 7 cut(s) 364, 413, 459, 769, 794, 995, 1273
HpyF10VI GCNNNNNNNGC 4 cut(s) 291, 320, 329, 1174
HpyF3I CTNAG 2 cut(s) 895, 1233
HpySE526I ACGT 3 cut(s) 368, 415, 1410
Hsp92I GRCGYC 1 cut(s) 368
Hsp92II CATG 9 cut(s) 385, 499, 696, 746, 882, 1168, 1226, 1343, 1373
HspAI GCGC 1 cut(s) 899
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
Ksp22I TGATCA 1 cut(s) 156
Kzo9I GATC 7 cut(s) 156, 352, 716, 802, 891, 1111, 1336
LmnI GCTCC 1 cut(s) 27
Lsp1109I GCAGC 3 cut(s) 1285, 1330, 1333
LweI GCATC 4 cut(s) 204, 400, 516, 1171
MaeI CTAG 1 cut(s) 1118
MaeII ACGT 3 cut(s) 368, 415, 1410
MaeIII GTNAC 2 cut(s) 101, 982
MalI GATC 7 cut(s) 158, 354, 718, 804, 893, 1113, 1338
MbiI CCGCTC 1 cut(s) 259
MboI GATC 7 cut(s) 156, 352, 716, 802, 891, 1111, 1336
MboII GAAGA 3 cut(s) 496, 681, 964
MflI RGATCY 2 cut(s) 716, 802
MhlI GDGCHC 3 cut(s) 166, 1015, 1201
MluCI AATT 6 cut(s) 116, 700, 738, 1021, 1259, 1386
MmeI TCCRAC 1 cut(s) 52
MreI CGCCGGCG 1 cut(s) 281
MroNI GCCGGC 1 cut(s) 281
MseI TTAA 5 cut(s) 864, 1050, 1137, 1158, 1380
MslI CAYNNNNRTG 1 cut(s) 747
MspCI CTTAAG 2 cut(s) 1157, 1379
MspI CCGG 3 cut(s) 282, 356, 530
MvnI CGCG 1 cut(s) 339
MwoI GCNNNNNNNGC 4 cut(s) 291, 320, 329, 1174
NaeI GCCGGC 1 cut(s) 283
NdeII GATC 7 cut(s) 156, 352, 716, 802, 891, 1111, 1336
NgoMIV GCCGGC 1 cut(s) 281
NlaIII CATG 9 cut(s) 385, 499, 696, 746, 882, 1168, 1226, 1343, 1373
NlaIV GGNNCC 1 cut(s) 1228
NspI RCATGY 3 cut(s) 385, 1168, 1373
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 267
PdiI GCCGGC 1 cut(s) 283
PfeI GAWTC 1 cut(s) 1219
PkrI GCNGC 6 cut(s) 287, 341, 972, 1275, 1345, 1348
Ppu21I YACGTR 1 cut(s) 416
PspFI CCCAGC 2 cut(s) 1237, 1319
PspN4I GGNNCC 1 cut(s) 1228
PspPI GGNCC 1 cut(s) 1227
PsuI RGATCY 2 cut(s) 716, 802
RsaI GTAC 2 cut(s) 877, 1368
RsaNI GTAC 2 cut(s) 876, 1367
RseI CAYNNNNRTG 1 cut(s) 747
SaqAI TTAA 5 cut(s) 864, 1050, 1137, 1158, 1380
SatI GCNGC 6 cut(s) 286, 340, 971, 1274, 1344, 1347
Sau3AI GATC 7 cut(s) 156, 352, 716, 802, 891, 1111, 1336
Sau96I GGNCC 1 cut(s) 1227
SduI GDGCHC 3 cut(s) 166, 1015, 1201
SfaNI GCATC 4 cut(s) 204, 400, 516, 1171
SgrAI CRCCGGYG 1 cut(s) 281
SinI GGWCC 1 cut(s) 1227
SmiMI CAYNNNNRTG 1 cut(s) 747
SmlI CTYRAG 2 cut(s) 1157, 1379
SmoI CTYRAG 2 cut(s) 1157, 1379
Sse9I AATT 6 cut(s) 116, 700, 738, 1021, 1259, 1386
SsiI CCGC 7 cut(s) 73, 257, 285, 288, 339, 406, 970
SspMI CTAG 1 cut(s) 1118
StyI CCWWGG 1 cut(s) 1117
TaaI ACNGT 2 cut(s) 89, 444
TaiI ACGT 3 cut(s) 371, 418, 1413
TaqI TCGA 1 cut(s) 426
TasI AATT 6 cut(s) 116, 700, 738, 1021, 1259, 1386
TatI WGTACW 1 cut(s) 875
TauI GCSGC 3 cut(s) 288, 342, 973
TfiI GAWTC 1 cut(s) 1219
Tru1I TTAA 5 cut(s) 864, 1050, 1137, 1158, 1380
Tru9I TTAA 5 cut(s) 864, 1050, 1137, 1158, 1380
TscAI CASTG 1 cut(s) 1237
TseI GCWGC 3 cut(s) 1273, 1343, 1346
TspDTI ATGAA 7 cut(s) 444, 678, 681, 731, 965, 1211, 1260
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 1 cut(s) 1237
Vha464I CTTAAG 2 cut(s) 1157, 1379
VpaK11BI GGWCC 1 cut(s) 1227
XapI RAATTY 4 cut(s) 116, 738, 1021, 1386
XceI RCATGY 3 cut(s) 385, 1168, 1373
XmaJI CCTAGG 1 cut(s) 1117
XspI CTAG 1 cut(s) 1118
ZraI GACGTC 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.