AT1G15400

at1g15400

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007633
Physical Location & Seq
Reverse (-)
5295756 .. 5297075
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G15400.1

Sequence Viewer

Length: 438 bp
ATGGAGGGTCTTCAAAGATCAACAATCTCTTTCCGCCGTCAGGGATCTTCCGGTATAGTGTTTGACGATCGTCTCATCGCCGAACTCAACAAATCGGGAAACAACGAGCAGAAAGACGAAAGTCAACGCGACGAACAACCTAAGCCTATGTCGGAAAGCTCTGAACAGGTCAAACCAATCGATGAGAAAGACAAGCTTAGACCGATTAAAACCGGTGGAGGAGCACCAGGAGGAATAGAGAGGAGTCGATCTAACGGTGGAGGAGCTCAACGACATCATAGAACAACCGGAAGAGTTTCTCCGGCAGTGGACCCGCCGTCTCCGAGAATTTCGTCATGCGGTTGTTGCTCTGCGTTTGGAAAGAATCCGCCGGGGAAGAAAGTTAATCCGAGGAAAAGGCCGCCGAAACGTAGATCGAGAGAAGGCGGCGGATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

145

Amino Acids

15.69

Weight (kDa)

10.27

Isoelectric Point (pI)

78.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 1 - 127 2.4e-37 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 129
AciI CCGC 7 cut(s) 34, 314, 339, 368, 401, 426, 429
AclWI GGATC 1 cut(s) 52
AcsI RAATTY 1 cut(s) 327
AfiI CCNNNNNNNGG 1 cut(s) 40
AgeI ACCGGT 1 cut(s) 212
AgsI TTSAA 1 cut(s) 14
AhdI GACNNNNNGTC 1 cut(s) 316
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 3 cut(s) 159, 196, 266
AluI AGCT 3 cut(s) 159, 196, 266
Alw21I GWGCWC 2 cut(s) 226, 268
Alw26I GTCTC 2 cut(s) 77, 324
AlwI GGATC 1 cut(s) 52
AoxI GGCC 1 cut(s) 398
ApoI RAATTY 1 cut(s) 327
AsiGI ACCGGT 1 cut(s) 212
Asp700I GAANNNNTTC 1 cut(s) 295
AspS9I GGNCC 1 cut(s) 310
AsuC2I CCSGG 1 cut(s) 372
AvaII GGWCC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 268
BbsI GAAGAC 1 cut(s) 2
Bbv12I GWGCWC 2 cut(s) 226, 268
BceAI ACGGC 2 cut(s) 21, 301
BciT130I CCWGG 1 cut(s) 228
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 2 cut(s) 77, 324
BisI GCNGC 2 cut(s) 401, 427
BlsI GCNGC 2 cut(s) 402, 428
Bme1390I CCNGG 2 cut(s) 228, 372
Bme18I GGWCC 1 cut(s) 310
BmeRI GACNNNNNGTC 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 1 cut(s) 312
BmrFI CCNGG 2 cut(s) 228, 372
BoxI GACNNNNGTC 2 cut(s) 69, 120
BpiI GAAGAC 1 cut(s) 2
Bpu10I CCTNAGC 1 cut(s) 141
BpuMI CCSGG 1 cut(s) 372
Bsa29I ATCGAT 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 371, 389
BsaWI WCCGGW 3 cut(s) 50, 212, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 40
Bse118I RCCGGY 1 cut(s) 212
BseBI CCWGG 1 cut(s) 228
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 2 cut(s) 371, 389
BseLI CCNNNNNNNGG 1 cut(s) 40
BseRI GAGGAG 3 cut(s) 234, 256, 276
Bsh1236I CGCG 1 cut(s) 129
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 1 cut(s) 400
BshTI ACCGGT 1 cut(s) 212
BshVI ATCGAT 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 2 cut(s) 226, 268
BsiSI CCGG 5 cut(s) 51, 213, 288, 302, 371
BslI CCNNNNNNNGG 1 cut(s) 40
BsmAI GTCTC 2 cut(s) 77, 324
BsmBI CGTCTC 2 cut(s) 77, 324
BsnI GGCC 1 cut(s) 400
Bsp1286I GDGCHC 2 cut(s) 226, 268
Bsp143I GATC 6 cut(s) 17, 44, 67, 248, 413, 431
BspACI CCGC 7 cut(s) 34, 314, 339, 368, 401, 426, 429
BspANI GGCC 1 cut(s) 400
BspDI ATCGAT 1 cut(s) 180
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 1 cut(s) 312
BspPI GGATC 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 2 cut(s) 371, 389
BssMI GATC 6 cut(s) 17, 44, 67, 248, 413, 431
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 257
Bst6I CTCTTC 1 cut(s) 286
BstDEI CTNAG 2 cut(s) 141, 197
BstFNI CGCG 1 cut(s) 129
BstKTI GATC 6 cut(s) 20, 47, 70, 251, 416, 434
BstMAI GTCTC 2 cut(s) 77, 324
BstMBI GATC 6 cut(s) 17, 44, 67, 248, 413, 431
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 1 cut(s) 345
BstNI CCWGG 1 cut(s) 228
BstPAI GACNNNNGTC 2 cut(s) 69, 120
BstSCI CCNGG 2 cut(s) 226, 370
BstUI CGCG 1 cut(s) 129
BstV2I GAAGAC 1 cut(s) 2
BstX2I RGATCY 1 cut(s) 44
BstYI RGATCY 1 cut(s) 44
Bsu15I ATCGAT 1 cut(s) 180
BsuRI GGCC 1 cut(s) 400
BsuTUI ATCGAT 1 cut(s) 180
BtgZI GCGATG 1 cut(s) 61
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 1 cut(s) 312
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 310
ClaI ATCGAT 1 cut(s) 180
CspAI ACCGGT 1 cut(s) 212
CviAII CATG 1 cut(s) 336
CviJI RGCY 5 cut(s) 145, 159, 196, 266, 400
CviKI_1 RGCY 5 cut(s) 145, 159, 196, 266, 400
DdeI CTNAG 2 cut(s) 141, 197
DpnI GATC 6 cut(s) 19, 46, 69, 250, 415, 433
DpnII GATC 6 cut(s) 17, 44, 67, 248, 413, 431
DriI GACNNNNNGTC 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 286
Eam1105I GACNNNNNGTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 286
EciI GGCGGA 2 cut(s) 23, 357
Ecl136II GAGCTC 1 cut(s) 266
Eco24I GRGCYC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 266
EcoRII CCWGG 1 cut(s) 226
EcoT38I GRGCYC 1 cut(s) 268
Esp3I CGTCTC 2 cut(s) 77, 324
FaeI CATG 1 cut(s) 339
FaiI YATR 4 cut(s) 56, 149, 279, 337
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FatI CATG 1 cut(s) 335
FauI CCCGC 1 cut(s) 321
Fnu4HI GCNGC 2 cut(s) 401, 427
FriOI GRGCYC 1 cut(s) 268
Fsp4HI GCNGC 2 cut(s) 401, 427
GluI GCNGC 2 cut(s) 401, 427
HaeIII GGCC 1 cut(s) 400
HapII CCGG 5 cut(s) 51, 213, 288, 302, 371
Hin1II CATG 1 cut(s) 339
HincII GTYRAC 1 cut(s) 125
HindII GTYRAC 1 cut(s) 125
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 2 cut(s) 244, 364
HpaII CCGG 5 cut(s) 51, 213, 288, 302, 371
Hpy166II GTNNAC 2 cut(s) 125, 310
Hpy188I TCNGA 4 cut(s) 154, 163, 324, 390
Hpy188III TCNNGA 3 cut(s) 96, 417, 435
Hpy8I GTNNAC 2 cut(s) 125, 310
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 1 cut(s) 257
HpyCH4IV ACGT 1 cut(s) 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 345
HpyF3I CTNAG 2 cut(s) 141, 197
HpySE526I ACGT 1 cut(s) 409
Hsp92II CATG 1 cut(s) 339
Kzo9I GATC 6 cut(s) 17, 44, 67, 248, 413, 431
LmnI GCTCC 2 cut(s) 221, 263
LpnPI CCDG 9 cut(s) 26, 64, 152, 213, 226, 240, 301, 315, 384
MaeII ACGT 1 cut(s) 409
MalI GATC 6 cut(s) 19, 46, 69, 250, 415, 433
MboI GATC 6 cut(s) 17, 44, 67, 248, 413, 431
MboII GAAGA 3 cut(s) 39, 303, 388
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 2 cut(s) 226, 268
MluCI AATT 1 cut(s) 327
MlyI GAGTC 1 cut(s) 253
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 5 cut(s) 212, 224, 234, 254, 384
MroXI GAANNNNTTC 1 cut(s) 295
MseI TTAA 2 cut(s) 207, 384
MspI CCGG 5 cut(s) 51, 213, 288, 302, 371
MspR9I CCNGG 2 cut(s) 228, 372
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 1 cut(s) 345
NciI CCSGG 1 cut(s) 372
NdeII GATC 6 cut(s) 17, 44, 67, 248, 413, 431
NlaIII CATG 1 cut(s) 339
NlaIV GGNNCC 1 cut(s) 312
PdmI GAANNNNTTC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 364
PinAI ACCGGT 1 cut(s) 212
PkrI GCNGC 2 cut(s) 402, 428
Ple19I CGATCG 1 cut(s) 70
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
PshAI GACNNNNGTC 2 cut(s) 69, 120
Psp124BI GAGCTC 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 312
PspPI GGNCC 1 cut(s) 310
PsuI RGATCY 1 cut(s) 44
PvuI CGATCG 1 cut(s) 70
SacI GAGCTC 1 cut(s) 268
SaqAI TTAA 2 cut(s) 207, 384
SatI GCNGC 2 cut(s) 401, 427
Sau3AI GATC 6 cut(s) 17, 44, 67, 248, 413, 431
Sau96I GGNCC 1 cut(s) 310
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 2 cut(s) 228, 372
SduI GDGCHC 2 cut(s) 226, 268
SetI ASST 6 cut(s) 142, 161, 171, 198, 268, 412
SinI GGWCC 1 cut(s) 310
Sse9I AATT 1 cut(s) 327
SsiI CCGC 7 cut(s) 34, 314, 339, 368, 401, 426, 429
SstI GAGCTC 1 cut(s) 268
StyD4I CCNGG 2 cut(s) 226, 370
TaaI ACNGT 1 cut(s) 257
TaiI ACGT 1 cut(s) 412
TaqI TCGA 3 cut(s) 180, 247, 416
TaqII GACCGA 1 cut(s) 217
TasI AATT 1 cut(s) 327
TauI GCSGC 2 cut(s) 403, 429
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 2 cut(s) 207, 384
Tru9I TTAA 2 cut(s) 207, 384
TscAI CASTG 1 cut(s) 312
TspRI CASTG 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 1 cut(s) 327
XmnI GAANNNNTTC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.