pycom810g00020

Domain of unknown function (DUF4666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000810
OG0007633
Physical Location & Seq
Forward (+)
42659 .. 42994
336 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom810g00020.1

Sequence Viewer

Length: 336 bp
ATGGCTGAGGGGCTTCAGAGGTCTGAGAGCACGTTTAGGAGGCAGGGCTCGTCGGGGTTGATTTGGGACGACAAGTTTCTACAGCGGGCGCTGAACCAAGTGGAGGCGGAGAAGCAGGCAGAGGCAGCTCAGCAGGCTCAGGGCGGCGGCACAGCAGCACCCACCATGGAGCGGAGCCGATCCAACGGAGGAAAGGGGTATCGGACGGTCAAAGTTTCGCCACAAGCGACGGACCCGCCTTCCCCGAAAGTCTCCGGCTGCGGGATCTGCGGCGCGTTTGGAAAACCGAAGCCGGCGTCGGCGCGTCGGCCGAAATCGAACAAACGAAGGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

112

Amino Acids

11.83

Weight (kDa)

10.53

Isoelectric Point (pI)

75.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 3 - 100 6.5e-30 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 172
AccII CGCG 2 cut(s) 275, 304
AciI CCGC 8 cut(s) 85, 107, 144, 147, 172, 236, 261, 270
AclWI GGATC 2 cut(s) 174, 272
AcoI YGGCCR 1 cut(s) 308
AcyI GRCGYC 1 cut(s) 296
AfiI CCNNNNNNNGG 3 cut(s) 103, 171, 261
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 256
AlwI GGATC 2 cut(s) 174, 272
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 3 cut(s) 125, 155, 258
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
AspLEI GCGC 3 cut(s) 91, 275, 304
AspS9I GGNCC 1 cut(s) 232
AvaII GGWCC 1 cut(s) 232
BanII GRGCYC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 3 cut(s) 137, 167, 245
BcoDI GTCTC 1 cut(s) 256
BfmI CTRYAG 1 cut(s) 80
BfoI RGCGCY 1 cut(s) 92
BisI GCNGC 6 cut(s) 126, 145, 148, 156, 259, 271
BlpI GCTNAGC 1 cut(s) 129
BlsI GCNGC 6 cut(s) 127, 146, 149, 157, 260, 272
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 2 cut(s) 176, 234
Bpu10I CCTNAGC 2 cut(s) 6, 138
Bpu1102I GCTNAGC 1 cut(s) 129
BsaHI GRCGYC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 165
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 171, 261
Bse118I RCCGGY 1 cut(s) 292
BseDI CCNNGG 1 cut(s) 165
BseLI CCNNNNNNNGG 3 cut(s) 103, 171, 261
BseMII CTCAG 3 cut(s) 15, 143, 152
BseX3I CGGCCG 1 cut(s) 308
BseXI GCAGC 3 cut(s) 137, 167, 245
Bsh1236I CGCG 2 cut(s) 275, 304
Bsh1285I CGRYCG 1 cut(s) 311
BshFI GGCC 1 cut(s) 310
BsiEI CGRYCG 1 cut(s) 311
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 2 cut(s) 255, 293
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 3 cut(s) 103, 171, 261
BsmAI GTCTC 1 cut(s) 256
BsmFI GGGAC 1 cut(s) 80
BsnI GGCC 1 cut(s) 310
Bsp1286I GDGCHC 2 cut(s) 32, 50
Bsp143I GATC 2 cut(s) 179, 264
Bsp1720I GCTNAGC 1 cut(s) 129
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 8 cut(s) 85, 107, 144, 147, 172, 236, 261, 270
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 3 cut(s) 16, 142, 151
BspFNI CGCG 2 cut(s) 275, 304
BspLI GGNNCC 2 cut(s) 176, 234
BspPI GGATC 2 cut(s) 174, 272
BsrBI CCGCTC 1 cut(s) 172
BsrFI RCCGGY 1 cut(s) 292
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 2 cut(s) 179, 264
BssNI GRCGYC 1 cut(s) 296
BssT1I CCWWGG 1 cut(s) 165
Bst4CI ACNGT 1 cut(s) 208
BstACI GRCGYC 1 cut(s) 296
BstC8I GCNNGC 4 cut(s) 87, 117, 135, 294
BstDEI CTNAG 4 cut(s) 6, 24, 129, 138
BstDSI CCRYGG 1 cut(s) 165
BstFNI CGCG 2 cut(s) 275, 304
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 3 cut(s) 91, 275, 304
BstKTI GATC 2 cut(s) 182, 267
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 2 cut(s) 179, 264
BstMCI CGRYCG 1 cut(s) 311
BstMWI GCNNNNNNNGC 3 cut(s) 125, 134, 267
BstSFI CTRYAG 1 cut(s) 80
BstUI CGCG 2 cut(s) 275, 304
BstV1I GCAGC 3 cut(s) 137, 167, 245
BstX2I RGATCY 1 cut(s) 264
BstYI RGATCY 1 cut(s) 264
BstZI CGGCCG 1 cut(s) 308
BsuRI GGCC 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 165
Cac8I GCNNGC 4 cut(s) 87, 117, 135, 294
CfoI GCGC 3 cut(s) 91, 275, 304
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 232
CseI GACGC 2 cut(s) 285, 293
CviAII CATG 1 cut(s) 166
CviJI RGCY 9 cut(s) 5, 13, 48, 128, 137, 177, 258, 292, 310
CviKI_1 RGCY 9 cut(s) 5, 13, 48, 128, 137, 177, 258, 292, 310
DdeI CTNAG 4 cut(s) 6, 24, 129, 138
DpnI GATC 2 cut(s) 181, 266
DpnII GATC 2 cut(s) 179, 264
EaeI YGGCCR 1 cut(s) 308
EagI CGGCCG 1 cut(s) 308
EciI GGCGGA 1 cut(s) 122
EclXI CGGCCG 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 50
Eco47I GGWCC 1 cut(s) 232
Eco52I CGGCCG 1 cut(s) 308
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 1 cut(s) 169
FaiI YATR 1 cut(s) 167
FaqI GGGAC 1 cut(s) 80
FatI CATG 1 cut(s) 165
FauI CCCGC 3 cut(s) 78, 243, 254
Fnu4HI GCNGC 6 cut(s) 126, 145, 148, 156, 259, 271
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 6 cut(s) 126, 145, 148, 156, 259, 271
GlaI GCGC 3 cut(s) 90, 274, 303
GluI GCNGC 6 cut(s) 126, 145, 148, 156, 259, 271
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 1 cut(s) 310
HapII CCGG 2 cut(s) 255, 293
HgaI GACGC 2 cut(s) 285, 293
HhaI GCGC 3 cut(s) 91, 275, 304
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 1 cut(s) 169
Hin6I GCGC 3 cut(s) 89, 273, 302
HinP1I GCGC 3 cut(s) 89, 273, 302
HpaII CCGG 2 cut(s) 255, 293
Hpy188I TCNGA 3 cut(s) 18, 25, 204
Hpy99I CGWCG 4 cut(s) 55, 232, 301, 309
HpyAV CCTTC 2 cut(s) 249, 321
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 1 cut(s) 32
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 134, 267
HpyF3I CTNAG 4 cut(s) 6, 24, 129, 138
HpySE526I ACGT 1 cut(s) 32
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 1 cut(s) 169
HspAI GCGC 3 cut(s) 89, 273, 302
KroI GCCGGC 1 cut(s) 292
KroNI GCCGGC 1 cut(s) 294
Kzo9I GATC 2 cut(s) 179, 264
LmnI GCTCC 2 cut(s) 169, 174
LpnPI CCDG 6 cut(s) 29, 101, 119, 125, 268, 306
Lsp1109I GCAGC 3 cut(s) 137, 167, 245
MaeII ACGT 1 cut(s) 32
MalI GATC 2 cut(s) 181, 266
MbiI CCGCTC 1 cut(s) 172
MboI GATC 2 cut(s) 179, 264
MflI RGATCY 1 cut(s) 264
MhlI GDGCHC 2 cut(s) 32, 50
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 5 cut(s) 12, 33, 97, 115, 182
MroNI GCCGGC 1 cut(s) 292
MspA1I CMGCKG 1 cut(s) 85
MspI CCGG 2 cut(s) 255, 293
MvnI CGCG 2 cut(s) 275, 304
MwoI GCNNNNNNNGC 3 cut(s) 125, 134, 267
NaeI GCCGGC 1 cut(s) 294
NcoI CCATGG 1 cut(s) 165
NdeII GATC 2 cut(s) 179, 264
NgoMIV GCCGGC 1 cut(s) 292
NlaIII CATG 1 cut(s) 169
NlaIV GGNNCC 2 cut(s) 176, 234
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PdiI GCCGGC 1 cut(s) 294
PkrI GCNGC 6 cut(s) 127, 146, 149, 157, 260, 272
PspN4I GGNNCC 2 cut(s) 176, 234
PspPI GGNCC 1 cut(s) 232
PsuI RGATCY 1 cut(s) 264
SatI GCNGC 6 cut(s) 126, 145, 148, 156, 259, 271
Sau3AI GATC 2 cut(s) 179, 264
Sau96I GGNCC 1 cut(s) 232
SduI GDGCHC 2 cut(s) 32, 50
SetI ASST 4 cut(s) 23, 35, 130, 332
SfcI CTRYAG 1 cut(s) 80
SinI GGWCC 1 cut(s) 232
SsiI CCGC 8 cut(s) 85, 107, 144, 147, 172, 236, 261, 270
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 1 cut(s) 35
TaqI TCGA 1 cut(s) 317
TauI GCSGC 3 cut(s) 147, 150, 273
TseI GCWGC 3 cut(s) 125, 155, 258
TspGWI ACGGA 2 cut(s) 201, 245
VpaK11BI GGWCC 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.