pycom15g15740

Domain of unknown function (DUF4666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007633
Physical Location & Seq
Reverse (-)
10956352 .. 10956675
324 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g15740.1

Sequence Viewer

Length: 324 bp
ATGGCTGAGGGGCTTCAGAGGTCTGAGAGCACGTTTAGGAGGCAGGGCTCGTCGGGCTTGATTTGGGACGACAAGTTTCTACAGCAGGCGCTGAACCAAGTGGAGGCGGAGAAGCAGGCGGAGGCAGCTCAGCAGGCTGAGGGCGGCGGCGGCGGCGCGGCAGGACCTACCATGGAGCGGAGCCGATCTGCAACGGAGGAAAGGGGTATCGGACGGTCAAGGTTTCGCCGCAAGCGATGGACCCGCCTTCCCCGAAAGTCTCCGGCTGCGGGATCTGCGGCGCCTTTGGAAAACCGAAGCCGGCGTCGGCGCGTCGGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

108

Amino Acids

11.83

Weight (kDa)

11.6

Isoelectric Point (pI)

103.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 3 - 78 4.8e-15 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 280
AccBSI CCGCTC 1 cut(s) 178
AccII CGCG 2 cut(s) 158, 312
AclWI GGATC 1 cut(s) 280
AcoI YGGCCR 1 cut(s) 316
AcyI GRCGYC 2 cut(s) 281, 304
AfiI CCNNNNNNNGG 3 cut(s) 103, 177, 269
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 264
AlwI GGATC 1 cut(s) 280
AlwNI CAGNNNCTG 1 cut(s) 91
AoxI GGCC 1 cut(s) 316
ApeKI GCWGC 2 cut(s) 125, 266
ArsI GACNNNNNNTTYG 2 cut(s) 289, 321
AspLEI GCGC 4 cut(s) 91, 158, 283, 312
AspS9I GGNCC 2 cut(s) 164, 240
AvaII GGWCC 2 cut(s) 164, 240
BanI GGYRCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 2 cut(s) 6, 138
BbvI GCAGC 2 cut(s) 137, 253
BccI CCATC 1 cut(s) 231
BcoDI GTCTC 1 cut(s) 264
BfmI CTRYAG 1 cut(s) 80
BfoI RGCGCY 2 cut(s) 92, 284
BisI GCNGC 9 cut(s) 126, 145, 148, 151, 154, 159, 229, 267, 279
BlpI GCTNAGC 1 cut(s) 129
BlsI GCNGC 9 cut(s) 127, 146, 149, 152, 155, 160, 230, 268, 280
Bme18I GGWCC 2 cut(s) 164, 240
BmgT120I GGNCC 2 cut(s) 164, 240
BmiI GGNNCC 3 cut(s) 182, 242, 282
Bpu10I CCTNAGC 2 cut(s) 6, 138
Bpu1102I GCTNAGC 1 cut(s) 129
BsaHI GRCGYC 2 cut(s) 281, 304
BsaJI CCNNGG 1 cut(s) 171
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 177, 269
Bse118I RCCGGY 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 171
BseLI CCNNNNNNNGG 3 cut(s) 103, 177, 269
BseMII CTCAG 3 cut(s) 15, 129, 143
BseXI GCAGC 2 cut(s) 137, 253
Bsh1236I CGCG 2 cut(s) 158, 312
BshFI GGCC 1 cut(s) 318
BshNI GGYRCC 1 cut(s) 280
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 2 cut(s) 263, 301
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 3 cut(s) 103, 177, 269
BsmAI GTCTC 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 80
BsnI GGCC 1 cut(s) 318
Bsp1286I GDGCHC 2 cut(s) 32, 50
Bsp143I GATC 2 cut(s) 185, 272
Bsp1720I GCTNAGC 1 cut(s) 129
Bsp19I CCATGG 1 cut(s) 171
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 3 cut(s) 16, 130, 142
BspFNI CGCG 2 cut(s) 158, 312
BspLI GGNNCC 3 cut(s) 182, 242, 282
BspPI GGATC 1 cut(s) 280
BspT107I GGYRCC 1 cut(s) 280
BsrBI CCGCTC 1 cut(s) 178
BsrFI RCCGGY 1 cut(s) 300
BssAI RCCGGY 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 2 cut(s) 185, 272
BssNI GRCGYC 2 cut(s) 281, 304
BssT1I CCWWGG 1 cut(s) 171
Bst4CI ACNGT 1 cut(s) 216
BstACI GRCGYC 2 cut(s) 281, 304
BstC8I GCNNGC 5 cut(s) 87, 117, 135, 233, 302
BstDEI CTNAG 4 cut(s) 6, 24, 129, 138
BstDSI CCRYGG 1 cut(s) 171
BstFNI CGCG 2 cut(s) 158, 312
BstH2I RGCGCY 2 cut(s) 92, 284
BstHHI GCGC 4 cut(s) 91, 158, 283, 312
BstKTI GATC 2 cut(s) 188, 275
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 2 cut(s) 185, 272
BstMWI GCNNNNNNNGC 6 cut(s) 54, 125, 134, 150, 153, 275
BstSFI CTRYAG 1 cut(s) 80
BstUI CGCG 2 cut(s) 158, 312
BstV1I GCAGC 2 cut(s) 137, 253
BstX2I RGATCY 1 cut(s) 272
BstYI RGATCY 1 cut(s) 272
BsuRI GGCC 1 cut(s) 318
BtgI CCRYGG 1 cut(s) 171
BtgZI GCGATG 1 cut(s) 250
Cac8I GCNNGC 5 cut(s) 87, 117, 135, 233, 302
CaiI CAGNNNCTG 1 cut(s) 91
CfoI GCGC 4 cut(s) 91, 158, 283, 312
Cfr10I RCCGGY 1 cut(s) 300
Cfr13I GGNCC 2 cut(s) 164, 240
CseI GACGC 2 cut(s) 293, 301
CviAII CATG 1 cut(s) 172
DdeI CTNAG 4 cut(s) 6, 24, 129, 138
DinI GGCGCC 1 cut(s) 282
DpnI GATC 2 cut(s) 187, 274
DpnII GATC 2 cut(s) 185, 272
EaeI YGGCCR 1 cut(s) 316
EciI GGCGGA 2 cut(s) 122, 134
Eco130I CCWWGG 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 50
Eco47I GGWCC 2 cut(s) 164, 240
EcoO109I RGGNCCY 1 cut(s) 164
EcoT14I CCWWGG 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 50
EgeI GGCGCC 1 cut(s) 282
EheI GGCGCC 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 1 cut(s) 175
FaiI YATR 1 cut(s) 173
FaqI GGGAC 1 cut(s) 80
FatI CATG 1 cut(s) 171
FauI CCCGC 2 cut(s) 251, 262
Fnu4HI GCNGC 9 cut(s) 126, 145, 148, 151, 154, 159, 229, 267, 279
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 9 cut(s) 126, 145, 148, 151, 154, 159, 229, 267, 279
GlaI GCGC 4 cut(s) 90, 157, 282, 311
GluI GCNGC 9 cut(s) 126, 145, 148, 151, 154, 159, 229, 267, 279
HaeII RGCGCY 2 cut(s) 92, 284
HaeIII GGCC 1 cut(s) 318
HapII CCGG 2 cut(s) 263, 301
HgaI GACGC 2 cut(s) 293, 301
HhaI GCGC 4 cut(s) 91, 158, 283, 312
Hin1I GRCGYC 2 cut(s) 281, 304
Hin1II CATG 1 cut(s) 175
Hin6I GCGC 4 cut(s) 89, 156, 281, 310
HinP1I GCGC 4 cut(s) 89, 156, 281, 310
HpaII CCGG 2 cut(s) 263, 301
Hpy188I TCNGA 3 cut(s) 18, 25, 212
Hpy99I CGWCG 3 cut(s) 55, 309, 317
HpyAV CCTTC 1 cut(s) 257
HpyCH4III ACNGT 1 cut(s) 216
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 1 cut(s) 191
HpyF10VI GCNNNNNNNGC 6 cut(s) 54, 125, 134, 150, 153, 275
HpyF3I CTNAG 4 cut(s) 6, 24, 129, 138
HpySE526I ACGT 1 cut(s) 32
Hsp92I GRCGYC 2 cut(s) 281, 304
Hsp92II CATG 1 cut(s) 175
HspAI GCGC 4 cut(s) 89, 156, 281, 310
KasI GGCGCC 1 cut(s) 280
KroI GCCGGC 1 cut(s) 300
KroNI GCCGGC 1 cut(s) 302
Kzo9I GATC 2 cut(s) 185, 272
LmnI GCTCC 2 cut(s) 175, 180
LpnPI CCDG 7 cut(s) 29, 71, 101, 119, 147, 276, 314
Lsp1109I GCAGC 2 cut(s) 137, 253
MaeII ACGT 1 cut(s) 32
MalI GATC 2 cut(s) 187, 274
MbiI CCGCTC 1 cut(s) 178
MboI GATC 2 cut(s) 185, 272
MflI RGATCY 1 cut(s) 272
MhlI GDGCHC 2 cut(s) 32, 50
Mly113I GGCGCC 1 cut(s) 281
MnlI CCTC 6 cut(s) 12, 33, 97, 115, 133, 190
MroNI GCCGGC 1 cut(s) 300
MspI CCGG 2 cut(s) 263, 301
MvnI CGCG 2 cut(s) 158, 312
MwoI GCNNNNNNNGC 6 cut(s) 54, 125, 134, 150, 153, 275
NaeI GCCGGC 1 cut(s) 302
NarI GGCGCC 1 cut(s) 281
NcoI CCATGG 1 cut(s) 171
NdeII GATC 2 cut(s) 185, 272
NgoMIV GCCGGC 1 cut(s) 300
NlaIII CATG 1 cut(s) 175
NlaIV GGNNCC 3 cut(s) 182, 242, 282
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PdiI GCCGGC 1 cut(s) 302
PkrI GCNGC 9 cut(s) 127, 146, 149, 152, 155, 160, 230, 268, 280
PluTI GGCGCC 1 cut(s) 284
PpuMI RGGWCCY 1 cut(s) 164
Psp5II RGGWCCY 1 cut(s) 164
PspN4I GGNNCC 3 cut(s) 182, 242, 282
PspPI GGNCC 2 cut(s) 164, 240
PspPPI RGGWCCY 1 cut(s) 164
PstNI CAGNNNCTG 1 cut(s) 91
PsuI RGATCY 1 cut(s) 272
SatI GCNGC 9 cut(s) 126, 145, 148, 151, 154, 159, 229, 267, 279
Sau3AI GATC 2 cut(s) 185, 272
Sau96I GGNCC 2 cut(s) 164, 240
SduI GDGCHC 2 cut(s) 32, 50
SetI ASST 5 cut(s) 23, 35, 130, 169, 224
SfcI CTRYAG 1 cut(s) 80
SfoI GGCGCC 1 cut(s) 282
SinI GGWCC 2 cut(s) 164, 240
SspDI GGCGCC 1 cut(s) 280
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 1 cut(s) 216
TaiI ACGT 1 cut(s) 35
TauI GCSGC 7 cut(s) 147, 150, 153, 156, 161, 231, 281
TseI GCWGC 2 cut(s) 125, 266
TspGWI ACGGA 1 cut(s) 209
VpaK11BI GGWCC 2 cut(s) 164, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.