RchiOBHm_Chr2g0089091

Domain of unknown function (DUF4666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007633
Physical Location & Seq
Reverse (-)
3406069 .. 3406917
849 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46439

Sequence Viewer

Length: 321 bp
ATGGCGGAATTGCAGAGGTCAGAGAGCACTTTCAGGAGGCAAGGATCTTCCGGTTTGATTTGGGACGACAAATTTCTACAGAGGGCTTTGAATCAAGTCGAGGCCGAGAAGCAAGCTGAAGCTGCCGCCGCTGCCGGCGGCGGCGGCTCCGACGGTCCTGCAGCTGACGGCGGAGCTCCAGGAGGGAGGGGGTATCGGACGGTCAAGGTTGCGCCGCCGATGATGGACCCGCCGTCGCCGAAGGTGTCCGGGTGTTGTGGAGTATTCGGAAAGCCGGCGGCGTCGAAGCGGCCCAGATCGAACCAGCGGAGATCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

106

Amino Acids

11.02

Weight (kDa)

10.02

Isoelectric Point (pI)

65.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 1 - 96 5.4e-23 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 52
AcsI RAATTY 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 138
AcyI GRCGYC 1 cut(s) 281
AgsI TTSAA 1 cut(s) 91
AhdI GACNNNNNGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 178
AluBI AGCT 4 cut(s) 116, 122, 164, 176
AluI AGCT 4 cut(s) 116, 122, 164, 176
Alw21I GWGCWC 2 cut(s) 29, 178
AlwI GGATC 1 cut(s) 52
AoxI GGCC 2 cut(s) 102, 290
ApeKI GCWGC 3 cut(s) 122, 131, 161
ApoI RAATTY 1 cut(s) 71
AspLEI GCGC 1 cut(s) 214
AspS9I GGNCC 3 cut(s) 155, 226, 291
AsuC2I CCSGG 1 cut(s) 250
AvaII GGWCC 2 cut(s) 155, 226
BanII GRGCYC 1 cut(s) 178
Bbv12I GWGCWC 2 cut(s) 29, 178
BbvI GCAGC 3 cut(s) 109, 118, 173
BccI CCATC 1 cut(s) 217
BceAI ACGGC 2 cut(s) 184, 217
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 1 cut(s) 180
BcnI CCSGG 1 cut(s) 250
BfmI CTRYAG 2 cut(s) 77, 159
Bme1390I CCNGG 2 cut(s) 180, 250
Bme18I GGWCC 2 cut(s) 155, 226
BmeRI GACNNNNNGTC 1 cut(s) 232
BmgT120I GGNCC 3 cut(s) 155, 226, 291
BmiI GGNNCC 2 cut(s) 148, 228
BmrFI CCNGG 2 cut(s) 180, 250
BpmI CTGGAG 1 cut(s) 162
BpuMI CCSGG 1 cut(s) 250
BsaHI GRCGYC 1 cut(s) 281
BsaWI WCCGGW 1 cut(s) 50
Bse118I RCCGGY 2 cut(s) 134, 274
BseBI CCWGG 1 cut(s) 180
BseXI GCAGC 3 cut(s) 109, 118, 173
BshFI GGCC 2 cut(s) 104, 292
BsiHKAI GWGCWC 2 cut(s) 29, 178
BsiSI CCGG 4 cut(s) 51, 135, 249, 275
BslFI GGGAC 1 cut(s) 77
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 2 cut(s) 104, 292
Bsp1286I GDGCHC 2 cut(s) 29, 178
Bsp143I GATC 3 cut(s) 44, 296, 311
BspANI GGCC 2 cut(s) 104, 292
BspLI GGNNCC 2 cut(s) 148, 228
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 1 cut(s) 52
BsrFI RCCGGY 2 cut(s) 134, 274
BssAI RCCGGY 2 cut(s) 134, 274
BssMI GATC 3 cut(s) 44, 296, 311
BssNI GRCGYC 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 180
Bst4CI ACNGT 2 cut(s) 155, 202
BstACI GRCGYC 1 cut(s) 281
BstC8I GCNNGC 3 cut(s) 114, 136, 276
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 3 cut(s) 47, 299, 314
BstMBI GATC 3 cut(s) 44, 296, 311
BstMWI GCNNNNNNNGC 4 cut(s) 122, 128, 131, 144
BstNI CCWGG 1 cut(s) 180
BstSCI CCNGG 2 cut(s) 178, 248
BstSFI CTRYAG 2 cut(s) 77, 159
BstV1I GCAGC 3 cut(s) 109, 118, 173
BstX2I RGATCY 1 cut(s) 44
BstYI RGATCY 1 cut(s) 44
BsuRI GGCC 2 cut(s) 104, 292
Cac8I GCNNGC 3 cut(s) 114, 136, 276
CfoI GCGC 1 cut(s) 214
Cfr10I RCCGGY 2 cut(s) 134, 274
Cfr13I GGNCC 3 cut(s) 155, 226, 291
CseI GACGC 1 cut(s) 270
CviJI RGCY 9 cut(s) 86, 104, 116, 122, 147, 164, 176, 274, 292
CviKI_1 RGCY 9 cut(s) 86, 104, 116, 122, 147, 164, 176, 274, 292
DpnI GATC 3 cut(s) 46, 298, 313
DpnII GATC 3 cut(s) 44, 296, 311
DriI GACNNNNNGTC 1 cut(s) 232
Eam1105I GACNNNNNGTC 1 cut(s) 232
EciI GGCGGA 2 cut(s) 20, 186
Ecl136II GAGCTC 1 cut(s) 176
Eco24I GRGCYC 1 cut(s) 178
Eco47I GGWCC 2 cut(s) 155, 226
Eco53kI GAGCTC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 138
EcoICRI GAGCTC 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 178
FaqI GGGAC 1 cut(s) 77
FauI CCCGC 1 cut(s) 237
FriOI GRGCYC 1 cut(s) 178
GlaI GCGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 162
HaeIII GGCC 2 cut(s) 104, 292
HapII CCGG 4 cut(s) 51, 135, 249, 275
HgaI GACGC 1 cut(s) 270
HhaI GCGC 1 cut(s) 214
Hin1I GRCGYC 1 cut(s) 281
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HinfI GANTC 1 cut(s) 91
HpaII CCGG 4 cut(s) 51, 135, 249, 275
Hpy188I TCNGA 4 cut(s) 22, 151, 198, 269
Hpy188III TCNNGA 1 cut(s) 34
Hpy99I CGWCG 3 cut(s) 155, 238, 286
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 2 cut(s) 155, 202
HpyCH4V TGCA 2 cut(s) 13, 161
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 128, 131, 144
Hsp92I GRCGYC 1 cut(s) 281
HspAI GCGC 1 cut(s) 212
KroI GCCGGC 2 cut(s) 134, 274
KroNI GCCGGC 2 cut(s) 136, 276
Kzo9I GATC 3 cut(s) 44, 296, 311
LmnI GCTCC 3 cut(s) 152, 173, 181
Lsp1109I GCAGC 3 cut(s) 109, 118, 173
MalI GATC 3 cut(s) 46, 298, 313
MboI GATC 3 cut(s) 44, 296, 311
MboII GAAGA 1 cut(s) 39
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 2 cut(s) 29, 178
MluCI AATT 2 cut(s) 8, 71
MmeI TCCRAC 1 cut(s) 174
MnlI CCTC 6 cut(s) 9, 30, 75, 94, 176, 180
MroNI GCCGGC 2 cut(s) 134, 274
MspA1I CMGCKG 3 cut(s) 131, 164, 307
MspI CCGG 4 cut(s) 51, 135, 249, 275
MspR9I CCNGG 2 cut(s) 180, 250
MvaI CCWGG 1 cut(s) 180
MwoI GCNNNNNNNGC 4 cut(s) 122, 128, 131, 144
NaeI GCCGGC 2 cut(s) 136, 276
NciI CCSGG 1 cut(s) 250
NdeII GATC 3 cut(s) 44, 296, 311
NgoMIV GCCGGC 2 cut(s) 134, 274
NlaIV GGNNCC 2 cut(s) 148, 228
NmeAIII GCCGAG 1 cut(s) 130
PdiI GCCGGC 2 cut(s) 136, 276
PfeI GAWTC 1 cut(s) 91
PfoI TCCNGGA 1 cut(s) 178
Psp124BI GAGCTC 1 cut(s) 178
Psp6I CCWGG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 178
PspN4I GGNNCC 2 cut(s) 148, 228
PspPI GGNCC 3 cut(s) 155, 226, 291
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 164
SacI GAGCTC 1 cut(s) 178
Sau3AI GATC 3 cut(s) 44, 296, 311
Sau96I GGNCC 3 cut(s) 155, 226, 291
ScrFI CCNGG 2 cut(s) 180, 250
SduI GDGCHC 2 cut(s) 29, 178
SetI ASST 7 cut(s) 20, 118, 124, 166, 178, 210, 246
SfcI CTRYAG 2 cut(s) 77, 159
SinI GGWCC 2 cut(s) 155, 226
Sse9I AATT 2 cut(s) 8, 71
SstI GAGCTC 1 cut(s) 178
StyD4I CCNGG 2 cut(s) 178, 248
TaaI ACNGT 2 cut(s) 155, 202
TaqI TCGA 4 cut(s) 99, 284, 299, 314
TasI AATT 2 cut(s) 8, 71
TauI GCSGC 8 cut(s) 128, 131, 141, 144, 147, 217, 281, 292
TfiI GAWTC 1 cut(s) 91
TseI GCWGC 3 cut(s) 122, 131, 161
VpaK11BI GGWCC 2 cut(s) 155, 226
XapI RAATTY 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.