AT1G80180

at1g15400

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007633
Physical Location & Seq
Reverse (-)
30156682 .. 30157808
1127 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G80180.1

Sequence Viewer

Length: 417 bp
ATGGCGGGTCTTCAGAGATCCACCATCTCTTTCCGTCGACAGGGCTCTTCGGGTATAGTCTGGGACGATCGTCTCATCGCCGAGCTAAGCCAACAAGCGGCCAACGACCGGAAAGGCGAGACTCTACAACAGGACGAACAGGCCAAGCTGATTACGTCGGAAGTACAGGATCAGACAACCAAACCTATCGCCGGAGAAGGACTCAAACCTATCAGAACTGACGGAGGTATGGAGAGGAGTCGATCCAACGGAGGAGGAGCCATCCGCCACCATCGAAACACAGGGAGAGTGTCTCCGGCGGTGGACCCGCCGTCGCCGAGACTGTCGGCGTTTGGGTGCTGCTCTGCCTTCGGGAAGAAACAGCCGGGGAAGAAGGTTAATCAGAGGAAAAGGCCAGCGAAGCGTAGATCCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

138

Amino Acids

15.11

Weight (kDa)

11.07

Isoelectric Point (pI)

64.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 1 - 125 5.5e-35 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 37
AciI CCGC 5 cut(s) 5, 98, 265, 299, 308
AclWI GGATC 4 cut(s) 12, 177, 237, 402
AcoI YGGCCR 1 cut(s) 99
AfaI GTAC 1 cut(s) 165
AfiI CCNNNNNNNGG 4 cut(s) 40, 97, 108, 191
AhdI GACNNNNNGTC 1 cut(s) 310
AluBI AGCT 2 cut(s) 85, 148
AluI AGCT 2 cut(s) 85, 148
Alw26I GTCTC 4 cut(s) 77, 113, 297, 313
AlwI GGATC 4 cut(s) 12, 177, 237, 402
AoxI GGCC 3 cut(s) 99, 141, 392
ApeKI GCWGC 1 cut(s) 339
AspS9I GGNCC 1 cut(s) 304
AsuC2I CCSGG 1 cut(s) 366
AvaII GGWCC 1 cut(s) 304
BanII GRGCYC 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 2
BbvI GCAGC 1 cut(s) 326
BccI CCATC 3 cut(s) 32, 269, 279
BceAI ACGGC 1 cut(s) 295
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 4 cut(s) 77, 113, 297, 313
BisI GCNGC 2 cut(s) 99, 340
BlpI GCTNAGC 1 cut(s) 86
BlsI GCNGC 2 cut(s) 100, 341
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 1 cut(s) 304
BmeRI GACNNNNNGTC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 304
BmiI GGNNCC 2 cut(s) 259, 306
BmrFI CCNGG 1 cut(s) 366
BoxI GACNNNNGTC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 2
BplI GAGNNNNNCTC 4 cut(s) 186, 218, 277, 309
Bpu1102I GCTNAGC 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 365
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 97, 108, 191
BseDI CCNNGG 1 cut(s) 365
BseGI GGATG 1 cut(s) 261
BseLI CCNNNNNNNGG 4 cut(s) 40, 97, 108, 191
BseRI GAGGAG 3 cut(s) 250, 267, 270
BseXI GCAGC 1 cut(s) 326
Bsh1285I CGRYCG 2 cut(s) 70, 109
BshFI GGCC 3 cut(s) 101, 143, 394
BsiEI CGRYCG 2 cut(s) 70, 109
BsiSI CCGG 4 cut(s) 109, 192, 296, 365
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 4 cut(s) 40, 97, 108, 191
BsmAI GTCTC 4 cut(s) 77, 113, 297, 313
BsmBI CGTCTC 1 cut(s) 77
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 3 cut(s) 101, 143, 394
Bsp1286I GDGCHC 1 cut(s) 47
Bsp143I GATC 5 cut(s) 17, 67, 169, 242, 407
Bsp1720I GCTNAGC 1 cut(s) 86
BspACI CCGC 5 cut(s) 5, 98, 265, 299, 308
BspANI GGCC 3 cut(s) 101, 143, 394
BspLI GGNNCC 2 cut(s) 259, 306
BspPI GGATC 4 cut(s) 12, 177, 237, 402
BspQI GCTCTTC 1 cut(s) 52
BssECI CCNNGG 1 cut(s) 365
BssMI GATC 5 cut(s) 17, 67, 169, 242, 407
Bst4CI ACNGT 1 cut(s) 324
Bst6I CTCTTC 1 cut(s) 52
BstC8I GCNNGC 1 cut(s) 396
BstDEI CTNAG 1 cut(s) 86
BstF5I GGATG 1 cut(s) 261
BstKTI GATC 5 cut(s) 20, 70, 172, 245, 410
BstMAI GTCTC 4 cut(s) 77, 113, 297, 313
BstMBI GATC 5 cut(s) 17, 67, 169, 242, 407
BstMCI CGRYCG 2 cut(s) 70, 109
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstPAI GACNNNNGTC 1 cut(s) 69
BstSCI CCNGG 1 cut(s) 364
BstV1I GCAGC 1 cut(s) 326
BstV2I GAAGAC 1 cut(s) 2
BstX2I RGATCY 2 cut(s) 17, 407
BstYI RGATCY 2 cut(s) 17, 407
BsuRI GGCC 3 cut(s) 101, 143, 394
BtgZI GCGATG 1 cut(s) 61
BtsCI GGATG 1 cut(s) 261
Cac8I GCNNGC 1 cut(s) 396
Cfr13I GGNCC 1 cut(s) 304
Csp6I GTAC 1 cut(s) 164
CviJI RGCY 9 cut(s) 45, 85, 90, 101, 143, 148, 260, 364, 394
CviKI_1 RGCY 9 cut(s) 45, 85, 90, 101, 143, 148, 260, 364, 394
CviQI GTAC 1 cut(s) 164
DdeI CTNAG 1 cut(s) 86
DpnI GATC 5 cut(s) 19, 69, 171, 244, 409
DpnII GATC 5 cut(s) 17, 67, 169, 242, 407
DriI GACNNNNNGTC 1 cut(s) 310
EaeI YGGCCR 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 52
Eam1105I GACNNNNNGTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 52
EciI GGCGGA 1 cut(s) 254
Eco24I GRGCYC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 47
Esp3I CGTCTC 1 cut(s) 77
FaiI YATR 2 cut(s) 56, 230
FaqI GGGAC 1 cut(s) 77
FauI CCCGC 1 cut(s) 315
FblI GTMKAC 1 cut(s) 37
Fnu4HI GCNGC 2 cut(s) 99, 340
FokI GGATG 1 cut(s) 248
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 2 cut(s) 99, 340
GluI GCNGC 2 cut(s) 99, 340
HaeIII GGCC 3 cut(s) 101, 143, 394
HapII CCGG 4 cut(s) 109, 192, 296, 365
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HinfI GANTC 3 cut(s) 121, 201, 238
HpaII CCGG 4 cut(s) 109, 192, 296, 365
Hpy166II GTNNAC 2 cut(s) 38, 304
Hpy188I TCNGA 5 cut(s) 15, 160, 174, 215, 384
Hpy188III TCNNGA 2 cut(s) 352, 411
Hpy8I GTNNAC 2 cut(s) 38, 304
Hpy99I CGWCG 3 cut(s) 39, 160, 316
HpyAV CCTTC 3 cut(s) 191, 358, 367
HpyCH4III ACNGT 1 cut(s) 324
HpyCH4IV ACGT 1 cut(s) 155
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 1 cut(s) 86
HpySE526I ACGT 1 cut(s) 155
Kzo9I GATC 5 cut(s) 17, 67, 169, 242, 407
LguI GCTCTTC 1 cut(s) 52
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 1 cut(s) 326
MaeII ACGT 1 cut(s) 155
MalI GATC 5 cut(s) 19, 69, 171, 244, 409
MboI GATC 5 cut(s) 17, 67, 169, 242, 407
MboII GAAGA 3 cut(s) 39, 367, 382
MflI RGATCY 2 cut(s) 17, 407
MhlI GDGCHC 1 cut(s) 47
MlyI GAGTC 3 cut(s) 115, 195, 247
MmeI TCCRAC 2 cut(s) 138, 270
MnlI CCTC 5 cut(s) 218, 228, 245, 248, 378
MseI TTAA 1 cut(s) 378
MspI CCGG 4 cut(s) 109, 192, 296, 365
MspR9I CCNGG 1 cut(s) 366
MwoI GCNNNNNNNGC 1 cut(s) 400
NciI CCSGG 1 cut(s) 366
NdeII GATC 5 cut(s) 17, 67, 169, 242, 407
NlaIV GGNNCC 2 cut(s) 259, 306
NmeAIII GCCGAG 2 cut(s) 106, 342
PciSI GCTCTTC 1 cut(s) 52
PkrI GCNGC 2 cut(s) 100, 341
Ple19I CGATCG 1 cut(s) 70
PleI GAGTC 3 cut(s) 115, 195, 246
PpsI GAGTC 3 cut(s) 115, 195, 246
PshAI GACNNNNGTC 1 cut(s) 69
PspN4I GGNNCC 2 cut(s) 259, 306
PspPI GGNCC 1 cut(s) 304
PsuI RGATCY 2 cut(s) 17, 407
PvuI CGATCG 1 cut(s) 70
RsaI GTAC 1 cut(s) 165
RsaNI GTAC 1 cut(s) 164
SalI GTCGAC 1 cut(s) 36
SapI GCTCTTC 1 cut(s) 52
SaqAI TTAA 1 cut(s) 378
SatI GCNGC 2 cut(s) 99, 340
Sau3AI GATC 5 cut(s) 17, 67, 169, 242, 407
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 3 cut(s) 115, 195, 247
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 1 cut(s) 47
SetI ASST 7 cut(s) 87, 150, 158, 187, 211, 229, 378
SinI GGWCC 1 cut(s) 304
SsiI CCGC 5 cut(s) 5, 98, 265, 299, 308
StyD4I CCNGG 1 cut(s) 364
TaaI ACNGT 1 cut(s) 324
TaiI ACGT 1 cut(s) 158
TaqI TCGA 3 cut(s) 37, 241, 274
TatI WGTACW 1 cut(s) 163
TauI GCSGC 1 cut(s) 101
Tru1I TTAA 1 cut(s) 378
Tru9I TTAA 1 cut(s) 378
TseI GCWGC 1 cut(s) 339
TspGWI ACGGA 3 cut(s) 23, 237, 264
VpaK11BI GGWCC 1 cut(s) 304
XmiI GTMKAC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.