pycom02g02900

Domain of unknown function (DUF4666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007633
Physical Location & Seq
Reverse (-)
1851355 .. 1851699
345 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02900.1

Sequence Viewer

Length: 345 bp
ATGGCTGAGGGGCTTCAGAGGTCTGAGAGCACGTTTAGGAGGCAGGGCTCGTCGGGGTTGATTTGGGACGACAAGTTTCTACAGCGGGCGCTGAACCAAGTGGAGGCGGAGAAGCAGGCAGAGGCAGCTCAGCAGGCTCAGGGCGGCGGCACAGCAGCACCACCATGGAGCGGAGCCGATCCAACGGAGGAAAGGGGTATCGGACGGTCAAAGTTTCGCCACAAGCGACGGACCCGCCTTCCCCGAAAGTCTCCGGCTGCGGGATCTGCGGCGCGTTTGGAAAACCGAAGCCGGCGTCGGCGCGTCGGCCGAAATCGAACAAACGAAGGTCGTAGAATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

115

Amino Acids

12.8

Weight (kDa)

11.8

Isoelectric Point (pI)

87.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 3 - 47 3.7e-13 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 171
AccII CGCG 2 cut(s) 274, 303
AciI CCGC 8 cut(s) 85, 107, 144, 147, 171, 235, 260, 269
AclWI GGATC 2 cut(s) 173, 271
AcoI YGGCCR 1 cut(s) 307
AcyI GRCGYC 1 cut(s) 295
AfiI CCNNNNNNNGG 3 cut(s) 103, 170, 260
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 2 cut(s) 173, 271
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 3 cut(s) 125, 155, 257
ArsI GACNNNNNNTTYG 2 cut(s) 280, 312
AseI ATTAAT 1 cut(s) 339
AspLEI GCGC 3 cut(s) 91, 274, 303
AspS9I GGNCC 1 cut(s) 231
AvaII GGWCC 1 cut(s) 231
BanII GRGCYC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 3 cut(s) 137, 167, 244
BcoDI GTCTC 1 cut(s) 255
BfmI CTRYAG 1 cut(s) 80
BfoI RGCGCY 1 cut(s) 92
BisI GCNGC 6 cut(s) 126, 145, 148, 156, 258, 270
BlpI GCTNAGC 1 cut(s) 129
BlsI GCNGC 6 cut(s) 127, 146, 149, 157, 259, 271
Bme18I GGWCC 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 2 cut(s) 175, 233
Bpu10I CCTNAGC 2 cut(s) 6, 138
Bpu1102I GCTNAGC 1 cut(s) 129
BsaHI GRCGYC 1 cut(s) 295
BsaJI CCNNGG 1 cut(s) 164
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 170, 260
Bse118I RCCGGY 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 164
BseLI CCNNNNNNNGG 3 cut(s) 103, 170, 260
BseMII CTCAG 3 cut(s) 15, 143, 152
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 3 cut(s) 137, 167, 244
Bsh1236I CGCG 2 cut(s) 274, 303
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 1 cut(s) 309
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 2 cut(s) 254, 292
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 3 cut(s) 103, 170, 260
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 80
BsnI GGCC 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 32, 50
Bsp143I GATC 2 cut(s) 178, 263
Bsp1720I GCTNAGC 1 cut(s) 129
Bsp19I CCATGG 1 cut(s) 164
BspACI CCGC 8 cut(s) 85, 107, 144, 147, 171, 235, 260, 269
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 3 cut(s) 16, 142, 151
BspFNI CGCG 2 cut(s) 274, 303
BspLI GGNNCC 2 cut(s) 175, 233
BspPI GGATC 2 cut(s) 173, 271
BsrBI CCGCTC 1 cut(s) 171
BsrFI RCCGGY 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 1 cut(s) 164
BssMI GATC 2 cut(s) 178, 263
BssNI GRCGYC 1 cut(s) 295
BssT1I CCWWGG 1 cut(s) 164
Bst4CI ACNGT 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 295
BstC8I GCNNGC 4 cut(s) 87, 117, 135, 293
BstDEI CTNAG 4 cut(s) 6, 24, 129, 138
BstDSI CCRYGG 1 cut(s) 164
BstFNI CGCG 2 cut(s) 274, 303
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 3 cut(s) 91, 274, 303
BstKTI GATC 2 cut(s) 181, 266
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 2 cut(s) 178, 263
BstMCI CGRYCG 1 cut(s) 310
BstMWI GCNNNNNNNGC 3 cut(s) 125, 134, 266
BstSFI CTRYAG 1 cut(s) 80
BstUI CGCG 2 cut(s) 274, 303
BstV1I GCAGC 3 cut(s) 137, 167, 244
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BstZI CGGCCG 1 cut(s) 307
BsuRI GGCC 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 164
Cac8I GCNNGC 4 cut(s) 87, 117, 135, 293
CfoI GCGC 3 cut(s) 91, 274, 303
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 231
CseI GACGC 2 cut(s) 284, 292
CviAII CATG 1 cut(s) 165
CviJI RGCY 9 cut(s) 5, 13, 48, 128, 137, 176, 257, 291, 309
CviKI_1 RGCY 9 cut(s) 5, 13, 48, 128, 137, 176, 257, 291, 309
DdeI CTNAG 4 cut(s) 6, 24, 129, 138
DpnI GATC 2 cut(s) 180, 265
DpnII GATC 2 cut(s) 178, 263
EaeI YGGCCR 1 cut(s) 307
EagI CGGCCG 1 cut(s) 307
EciI GGCGGA 1 cut(s) 122
EclXI CGGCCG 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 164
Eco24I GRGCYC 1 cut(s) 50
Eco47I GGWCC 1 cut(s) 231
Eco52I CGGCCG 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 164
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 164
FaeI CATG 1 cut(s) 168
FaiI YATR 1 cut(s) 166
FaqI GGGAC 1 cut(s) 80
FatI CATG 1 cut(s) 164
FauI CCCGC 3 cut(s) 78, 242, 253
Fnu4HI GCNGC 6 cut(s) 126, 145, 148, 156, 258, 270
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 6 cut(s) 126, 145, 148, 156, 258, 270
GlaI GCGC 3 cut(s) 90, 273, 302
GluI GCNGC 6 cut(s) 126, 145, 148, 156, 258, 270
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 1 cut(s) 309
HapII CCGG 2 cut(s) 254, 292
HgaI GACGC 2 cut(s) 284, 292
HhaI GCGC 3 cut(s) 91, 274, 303
Hin1I GRCGYC 1 cut(s) 295
Hin1II CATG 1 cut(s) 168
Hin6I GCGC 3 cut(s) 89, 272, 301
HinP1I GCGC 3 cut(s) 89, 272, 301
HpaII CCGG 2 cut(s) 254, 292
Hpy188I TCNGA 3 cut(s) 18, 25, 203
Hpy99I CGWCG 4 cut(s) 55, 231, 300, 308
HpyAV CCTTC 2 cut(s) 248, 320
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4IV ACGT 1 cut(s) 32
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 134, 266
HpyF3I CTNAG 4 cut(s) 6, 24, 129, 138
HpySE526I ACGT 1 cut(s) 32
Hsp92I GRCGYC 1 cut(s) 295
Hsp92II CATG 1 cut(s) 168
HspAI GCGC 3 cut(s) 89, 272, 301
KroI GCCGGC 1 cut(s) 291
KroNI GCCGGC 1 cut(s) 293
Kzo9I GATC 2 cut(s) 178, 263
LmnI GCTCC 2 cut(s) 168, 173
LpnPI CCDG 6 cut(s) 29, 101, 119, 125, 267, 305
Lsp1109I GCAGC 3 cut(s) 137, 167, 244
MaeII ACGT 1 cut(s) 32
MalI GATC 2 cut(s) 180, 265
MbiI CCGCTC 1 cut(s) 171
MboI GATC 2 cut(s) 178, 263
MflI RGATCY 1 cut(s) 263
MhlI GDGCHC 2 cut(s) 32, 50
MluCI AATT 2 cut(s) 336, 340
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 5 cut(s) 12, 33, 97, 115, 181
MroNI GCCGGC 1 cut(s) 291
MseI TTAA 2 cut(s) 339, 343
MslI CAYNNNNRTG 1 cut(s) 163
MspA1I CMGCKG 1 cut(s) 85
MspI CCGG 2 cut(s) 254, 292
MvnI CGCG 2 cut(s) 274, 303
MwoI GCNNNNNNNGC 3 cut(s) 125, 134, 266
NaeI GCCGGC 1 cut(s) 293
NcoI CCATGG 1 cut(s) 164
NdeII GATC 2 cut(s) 178, 263
NgoMIV GCCGGC 1 cut(s) 291
NlaIII CATG 1 cut(s) 168
NlaIV GGNNCC 2 cut(s) 175, 233
PacI TTAATTAA 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 223
PdiI GCCGGC 1 cut(s) 293
PkrI GCNGC 6 cut(s) 127, 146, 149, 157, 259, 271
PshBI ATTAAT 1 cut(s) 339
PspN4I GGNNCC 2 cut(s) 175, 233
PspPI GGNCC 1 cut(s) 231
PsuI RGATCY 1 cut(s) 263
RseI CAYNNNNRTG 1 cut(s) 163
SaqAI TTAA 2 cut(s) 339, 343
SatI GCNGC 6 cut(s) 126, 145, 148, 156, 258, 270
Sau3AI GATC 2 cut(s) 178, 263
Sau96I GGNCC 1 cut(s) 231
SduI GDGCHC 2 cut(s) 32, 50
SetI ASST 4 cut(s) 23, 35, 130, 331
SfcI CTRYAG 1 cut(s) 80
SinI GGWCC 1 cut(s) 231
SmiMI CAYNNNNRTG 1 cut(s) 163
Sse9I AATT 2 cut(s) 336, 340
SsiI CCGC 8 cut(s) 85, 107, 144, 147, 171, 235, 260, 269
StyI CCWWGG 1 cut(s) 164
TaaI ACNGT 1 cut(s) 207
TaiI ACGT 1 cut(s) 35
TaqI TCGA 1 cut(s) 316
TasI AATT 2 cut(s) 336, 340
TauI GCSGC 3 cut(s) 147, 150, 272
Tru1I TTAA 2 cut(s) 339, 343
Tru9I TTAA 2 cut(s) 339, 343
TseI GCWGC 3 cut(s) 125, 155, 257
TspGWI ACGGA 2 cut(s) 200, 244
VpaK11BI GGWCC 1 cut(s) 231
VspI ATTAAT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.