Rh2BG041300

Domain of unknown function (DUF4666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007633
Physical Location & Seq
Reverse (-)
2853624 .. 2855052
1429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG041300.1

Sequence Viewer

Length: 330 bp
ATGGCGGAATTGCAGAGGTCAGAGAGCACTTTCAGGAGGCAAGGATCTTCCGGTTTGATTTGGGACGACAAATTTCTACAGAGGGCTTTGAATCAAGTCGAGGCCGAGAAGCAAGCTGAAGCTGCCGCCGCTGCCAGCGGCGGCGGCGGAGGAGGCTCCGACGGTCCTGCAGCTGACGGCGGAGCTCCGGGAGGGAGGGGTTATCGGACGGTCAAGGTTGCGCCGCCGATGATGGACCCGCCGTCGCCGAAGGTCTCCGGGTGTTGCGGAGTATTCGGAAAGCCGGCGGCGTCGAAGCGGCCCAGATCGAACCAGCGGAGATCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4666

Protein Analysis

109

Amino Acids

11.22

Weight (kDa)

10.02

Isoelectric Point (pI)

66.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4666 PF15697 1 - 99 4e-24 Domain of unknown function (DUF4666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 52
AcsI RAATTY 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 138
AcyI GRCGYC 1 cut(s) 290
AgsI TTSAA 1 cut(s) 91
AhdI GACNNNNNGTC 1 cut(s) 241
AluBI AGCT 4 cut(s) 116, 122, 173, 185
AluI AGCT 4 cut(s) 116, 122, 173, 185
Alw21I GWGCWC 2 cut(s) 29, 187
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 1 cut(s) 52
AoxI GGCC 2 cut(s) 102, 299
ApeKI GCWGC 3 cut(s) 122, 131, 170
ApoI RAATTY 1 cut(s) 71
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 3 cut(s) 164, 235, 300
AsuC2I CCSGG 2 cut(s) 189, 259
AvaII GGWCC 2 cut(s) 164, 235
BanII GRGCYC 1 cut(s) 187
Bbv12I GWGCWC 2 cut(s) 29, 187
BbvI GCAGC 3 cut(s) 109, 118, 182
BccI CCATC 1 cut(s) 226
BceAI ACGGC 2 cut(s) 193, 226
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BcnI CCSGG 2 cut(s) 189, 259
BcoDI GTCTC 1 cut(s) 259
BfmI CTRYAG 2 cut(s) 77, 168
Bme1390I CCNGG 2 cut(s) 189, 259
Bme18I GGWCC 2 cut(s) 164, 235
BmeRI GACNNNNNGTC 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 164, 235, 300
BmiI GGNNCC 2 cut(s) 157, 237
BmrFI CCNGG 2 cut(s) 189, 259
BpuMI CCSGG 2 cut(s) 189, 259
BsaHI GRCGYC 1 cut(s) 290
BsaI GGTCTC 1 cut(s) 259
BsaWI WCCGGW 1 cut(s) 50
Bse118I RCCGGY 1 cut(s) 283
BseRI GAGGAG 1 cut(s) 165
BseXI GCAGC 3 cut(s) 109, 118, 182
BshFI GGCC 2 cut(s) 104, 301
BsiHKAI GWGCWC 2 cut(s) 29, 187
BsiSI CCGG 4 cut(s) 51, 188, 258, 284
BslFI GGGAC 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 259
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 2 cut(s) 104, 301
Bso31I GGTCTC 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 29, 187
Bsp143I GATC 3 cut(s) 44, 305, 320
BspANI GGCC 2 cut(s) 104, 301
BspLI GGNNCC 2 cut(s) 157, 237
BspMAI CTGCAG 1 cut(s) 172
BspPI GGATC 1 cut(s) 52
BspTNI GGTCTC 1 cut(s) 259
BsrFI RCCGGY 1 cut(s) 283
BssAI RCCGGY 1 cut(s) 283
BssMI GATC 3 cut(s) 44, 305, 320
BssNI GRCGYC 1 cut(s) 290
Bst4CI ACNGT 2 cut(s) 164, 211
BstACI GRCGYC 1 cut(s) 290
BstC8I GCNNGC 3 cut(s) 114, 136, 285
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 3 cut(s) 47, 308, 323
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 3 cut(s) 44, 305, 320
BstMWI GCNNNNNNNGC 5 cut(s) 122, 128, 131, 144, 153
BstSCI CCNGG 2 cut(s) 187, 257
BstSFI CTRYAG 2 cut(s) 77, 168
BstV1I GCAGC 3 cut(s) 109, 118, 182
BstX2I RGATCY 1 cut(s) 44
BstYI RGATCY 1 cut(s) 44
BsuRI GGCC 2 cut(s) 104, 301
Cac8I GCNNGC 3 cut(s) 114, 136, 285
CfoI GCGC 1 cut(s) 223
Cfr10I RCCGGY 1 cut(s) 283
Cfr13I GGNCC 3 cut(s) 164, 235, 300
CseI GACGC 1 cut(s) 279
CviJI RGCY 9 cut(s) 86, 104, 116, 122, 156, 173, 185, 283, 301
CviKI_1 RGCY 9 cut(s) 86, 104, 116, 122, 156, 173, 185, 283, 301
DpnI GATC 3 cut(s) 46, 307, 322
DpnII GATC 3 cut(s) 44, 305, 320
DriI GACNNNNNGTC 1 cut(s) 241
Eam1105I GACNNNNNGTC 1 cut(s) 241
EciI GGCGGA 3 cut(s) 20, 162, 195
Ecl136II GAGCTC 1 cut(s) 185
Eco24I GRGCYC 1 cut(s) 187
Eco31I GGTCTC 1 cut(s) 259
Eco47I GGWCC 2 cut(s) 164, 235
Eco53kI GAGCTC 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 138
EcoICRI GAGCTC 1 cut(s) 185
EcoT38I GRGCYC 1 cut(s) 187
FaqI GGGAC 1 cut(s) 77
FauI CCCGC 1 cut(s) 246
FriOI GRGCYC 1 cut(s) 187
GlaI GCGC 1 cut(s) 222
HaeIII GGCC 2 cut(s) 104, 301
HapII CCGG 4 cut(s) 51, 188, 258, 284
HgaI GACGC 1 cut(s) 279
HhaI GCGC 1 cut(s) 223
Hin1I GRCGYC 1 cut(s) 290
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HinfI GANTC 1 cut(s) 91
HpaII CCGG 4 cut(s) 51, 188, 258, 284
Hpy188I TCNGA 4 cut(s) 22, 160, 207, 278
Hpy188III TCNNGA 1 cut(s) 34
Hpy99I CGWCG 3 cut(s) 164, 247, 295
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 2 cut(s) 164, 211
HpyCH4V TGCA 2 cut(s) 13, 170
HpyF10VI GCNNNNNNNGC 5 cut(s) 122, 128, 131, 144, 153
Hsp92I GRCGYC 1 cut(s) 290
HspAI GCGC 1 cut(s) 221
KroI GCCGGC 1 cut(s) 283
KroNI GCCGGC 1 cut(s) 285
Kzo9I GATC 3 cut(s) 44, 305, 320
LmnI GCTCC 3 cut(s) 161, 182, 190
LpnPI CCDG 9 cut(s) 19, 64, 148, 180, 201, 271, 297, 316, 326
Lsp1109I GCAGC 3 cut(s) 109, 118, 182
MalI GATC 3 cut(s) 46, 307, 322
MboI GATC 3 cut(s) 44, 305, 320
MboII GAAGA 1 cut(s) 39
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 2 cut(s) 29, 187
MluCI AATT 2 cut(s) 8, 71
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 8 cut(s) 9, 30, 75, 94, 143, 146, 185, 189
MroNI GCCGGC 1 cut(s) 283
MspA1I CMGCKG 4 cut(s) 131, 138, 173, 316
MspI CCGG 4 cut(s) 51, 188, 258, 284
MspR9I CCNGG 2 cut(s) 189, 259
MwoI GCNNNNNNNGC 5 cut(s) 122, 128, 131, 144, 153
NaeI GCCGGC 1 cut(s) 285
NciI CCSGG 2 cut(s) 189, 259
NdeII GATC 3 cut(s) 44, 305, 320
NgoMIV GCCGGC 1 cut(s) 283
NlaIV GGNNCC 2 cut(s) 157, 237
NmeAIII GCCGAG 1 cut(s) 130
PdiI GCCGGC 1 cut(s) 285
PfeI GAWTC 1 cut(s) 91
PfoI TCCNGGA 1 cut(s) 187
Psp124BI GAGCTC 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 157, 237
PspPI GGNCC 3 cut(s) 164, 235, 300
PstI CTGCAG 1 cut(s) 172
PsuI RGATCY 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 173
SacI GAGCTC 1 cut(s) 187
Sau3AI GATC 3 cut(s) 44, 305, 320
Sau96I GGNCC 3 cut(s) 164, 235, 300
ScrFI CCNGG 2 cut(s) 189, 259
SduI GDGCHC 2 cut(s) 29, 187
SetI ASST 7 cut(s) 20, 118, 124, 175, 187, 219, 255
SfcI CTRYAG 2 cut(s) 77, 168
SinI GGWCC 2 cut(s) 164, 235
Sse9I AATT 2 cut(s) 8, 71
SstI GAGCTC 1 cut(s) 187
StyD4I CCNGG 2 cut(s) 187, 257
TaaI ACNGT 2 cut(s) 164, 211
TaqI TCGA 4 cut(s) 99, 293, 308, 323
TasI AATT 2 cut(s) 8, 71
TauI GCSGC 8 cut(s) 128, 131, 141, 144, 147, 226, 290, 301
TfiI GAWTC 1 cut(s) 91
TseI GCWGC 3 cut(s) 122, 131, 170
VpaK11BI GGWCC 2 cut(s) 164, 235
XapI RAATTY 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.