AT1G18680

HNH nucleases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010466
Physical Location & Seq
Reverse (-)
6431938 .. 6433552
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G18680.1

Sequence Viewer

Length: 561 bp
ATGGGGTCATCTTTCTCCGCCTCCTTTACTAACTCCACCACCGCCGCCGCCGTTCCTCCTCCGTCGCCACCTTCATCGCCGTCACGTTCCAATGTAAAATCTAACGGAGAAGAGAGACCTCGTTTCTTCGATGGAAAGGCGAAGAACAAGTGCTGGGCTAATGCTGACATCGTACCTGGTCGACATCCCGAGAGGTGGCGTAAAGACGTCGCCGGTAACATAGTCTGCAAACGCTTCGGAAATTGCAATGGTTGTCTCTGTTTCGAGTATGATCACATTGTTCCTTATTCCAAAGGTGGAGAGTCGATAGCGGAGAATTGTCAGATTCTTCAAACAAGAGTTAACAGATTCAAATCAGCTCAAGAAAATGTTGATGCCACCACTCTTAAGAGCTATTCTTGTGGTCTGCAATTTACGGACAAGGAGTTAGATGTTATCGAGATGGCGGTTTATGGAGACGTGTTACGACCTGGAAAAGAATGTCGCTGCAAAACCGTAGCTGAGCTGCTTGGTCAGTCCAAGTCCAAAGATGGTAAAGCCGCATGCGAGTTACCATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH

Protein Analysis

186

Amino Acids

20.29

Weight (kDa)

8.67

Isoelectric Point (pI)

58.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 210
AccI GTMKAC 1 cut(s) 181
AciI CCGC 7 cut(s) 18, 42, 45, 48, 311, 446, 540
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 1 cut(s) 174
AfiI CCNNNNNNNGG 1 cut(s) 195
AflII CTTAAG 1 cut(s) 386
AflIII ACRYGT 1 cut(s) 459
AgsI TTSAA 2 cut(s) 332, 352
AjiI CACGTC 1 cut(s) 460
AjnI CCWGG 2 cut(s) 175, 469
AluBI AGCT 4 cut(s) 359, 393, 500, 505
AluI AGCT 4 cut(s) 359, 393, 500, 505
Alw26I GTCTC 3 cut(s) 109, 260, 450
Ama87I CYCGRG 1 cut(s) 188
ApeKI GCWGC 2 cut(s) 486, 505
AvaI CYCGRG 1 cut(s) 188
BbvI GCAGC 2 cut(s) 473, 492
BccI CCATC 3 cut(s) 125, 436, 524
BceAI ACGGC 2 cut(s) 35, 64
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 2 cut(s) 177, 471
BclI TGATCA 1 cut(s) 271
BcoDI GTCTC 3 cut(s) 109, 260, 450
BfrI CTTAAG 1 cut(s) 386
BisI GCNGC 5 cut(s) 45, 48, 487, 506, 540
BlpI GCTNAGC 1 cut(s) 501
BlsI GCNGC 5 cut(s) 46, 49, 488, 507, 541
Bme1390I CCNGG 2 cut(s) 177, 471
BmeT110I CYCGRG 1 cut(s) 188
BmgBI CACGTC 1 cut(s) 460
BmrFI CCNGG 2 cut(s) 177, 471
BmsI GCATC 1 cut(s) 364
Bpu1102I GCTNAGC 1 cut(s) 501
BpuEI CTTGAG 1 cut(s) 345
BsaBI GATNNNNATC 1 cut(s) 352
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 109
Bsc4I CCNNNNNNNGG 1 cut(s) 195
Bse118I RCCGGY 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 253
Bse8I GATNNNNATC 1 cut(s) 352
BseBI CCWGG 2 cut(s) 177, 471
BseGI GGATG 1 cut(s) 184
BseJI GATNNNNATC 1 cut(s) 352
BseLI CCNNNNNNNGG 1 cut(s) 195
BseMI GCAATG 1 cut(s) 253
BseMII CTCAG 1 cut(s) 492
BseRI GAGGAG 1 cut(s) 48
BseXI GCAGC 2 cut(s) 473, 492
BseYI CCCAGC 1 cut(s) 153
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 1 cut(s) 213
BslI CCNNNNNNNGG 1 cut(s) 195
BsmAI GTCTC 3 cut(s) 109, 260, 450
BsmBI CGTCTC 1 cut(s) 450
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 188
Bsp143I GATC 1 cut(s) 271
Bsp1720I GCTNAGC 1 cut(s) 501
BspACI CCGC 7 cut(s) 18, 42, 45, 48, 311, 446, 540
BspCNI CTCAG 1 cut(s) 493
BspTI CTTAAG 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 253
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssMI GATC 1 cut(s) 271
BssNI GRCGYC 1 cut(s) 207
Bst2UI CCWGG 2 cut(s) 177, 471
Bst4CI ACNGT 1 cut(s) 496
Bst6I CTCTTC 1 cut(s) 105
BstACI GRCGYC 1 cut(s) 207
BstAFI CTTAAG 1 cut(s) 386
BstC8I GCNNGC 1 cut(s) 544
BstDEI CTNAG 1 cut(s) 501
BstF5I GGATG 1 cut(s) 184
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 3 cut(s) 109, 260, 450
BstMBI GATC 1 cut(s) 271
BstNI CCWGG 2 cut(s) 177, 471
BstNSI RCATGY 1 cut(s) 546
BstSCI CCNGG 2 cut(s) 175, 469
BstV1I GCAGC 2 cut(s) 473, 492
BtgZI GCGATG 1 cut(s) 60
BtrI CACGTC 1 cut(s) 460
BtsCI GGATG 1 cut(s) 184
Cac8I GCNNGC 1 cut(s) 544
Cfr10I RCCGGY 1 cut(s) 212
CsiI ACCWGGT 1 cut(s) 175
Csp6I GTAC 1 cut(s) 173
CviAII CATG 1 cut(s) 543
CviJI RGCY 6 cut(s) 158, 359, 393, 500, 505, 539
CviKI_1 RGCY 6 cut(s) 158, 359, 393, 500, 505, 539
CviQI GTAC 1 cut(s) 173
DdeI CTNAG 1 cut(s) 501
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
EciI GGCGGA 1 cut(s) 7
Eco31I GGTCTC 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 188
EcoRII CCWGG 2 cut(s) 175, 469
Esp3I CGTCTC 1 cut(s) 450
FaeI CATG 1 cut(s) 546
FaiI YATR 5 cut(s) 221, 270, 453, 544, 559
FatI CATG 1 cut(s) 542
FbaI TGATCA 1 cut(s) 271
FblI GTMKAC 1 cut(s) 181
Fnu4HI GCNGC 5 cut(s) 45, 48, 487, 506, 540
FokI GGATG 1 cut(s) 171
Fsp4HI GCNGC 5 cut(s) 45, 48, 487, 506, 540
GluI GCNGC 5 cut(s) 45, 48, 487, 506, 540
GsaI CCCAGC 1 cut(s) 157
HapII CCGG 1 cut(s) 213
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 1 cut(s) 546
HincII GTYRAC 2 cut(s) 182, 343
HindII GTYRAC 2 cut(s) 182, 343
HinfI GANTC 3 cut(s) 302, 325, 348
HpaI GTTAAC 1 cut(s) 343
HpaII CCGG 1 cut(s) 213
Hpy166II GTNNAC 2 cut(s) 182, 343
Hpy188I TCNGA 2 cut(s) 239, 324
Hpy188III TCNNGA 3 cut(s) 188, 362, 439
Hpy8I GTNNAC 2 cut(s) 182, 343
Hpy99I CGWCG 2 cut(s) 67, 212
HpyAV CCTTC 1 cut(s) 81
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4IV ACGT 3 cut(s) 85, 207, 459
HpyCH4V TGCA 4 cut(s) 228, 246, 409, 489
HpyF3I CTNAG 1 cut(s) 501
HpySE526I ACGT 3 cut(s) 85, 207, 459
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 1 cut(s) 546
Ksp22I TGATCA 1 cut(s) 271
KspAI GTTAAC 1 cut(s) 343
Kzo9I GATC 1 cut(s) 271
LpnPI CCDG 6 cut(s) 139, 162, 189, 226, 456, 483
Lsp1109I GCAGC 2 cut(s) 473, 492
LweI GCATC 1 cut(s) 364
MabI ACCWGGT 1 cut(s) 175
MaeII ACGT 3 cut(s) 85, 207, 459
MaeIII GTNAC 4 cut(s) 81, 215, 462, 549
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MboII GAAGA 4 cut(s) 118, 122, 154, 320
MluCI AATT 3 cut(s) 241, 316, 410
MlyI GAGTC 1 cut(s) 311
MnlI CCTC 5 cut(s) 31, 66, 69, 129, 186
MseI TTAA 2 cut(s) 342, 387
MspCI CTTAAG 1 cut(s) 386
MspI CCGG 1 cut(s) 213
MspR9I CCNGG 2 cut(s) 177, 471
MvaI CCWGG 2 cut(s) 177, 471
NdeII GATC 1 cut(s) 271
NlaIII CATG 1 cut(s) 546
NmuCI GTSAC 1 cut(s) 81
NspI RCATGY 1 cut(s) 546
PaeI GCATGC 1 cut(s) 546
PfeI GAWTC 2 cut(s) 325, 348
PkrI GCNGC 5 cut(s) 46, 49, 488, 507, 541
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
Psp6I CCWGG 2 cut(s) 175, 469
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 2 cut(s) 175, 469
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
SalI GTCGAC 1 cut(s) 180
SaqAI TTAA 2 cut(s) 342, 387
SatI GCNGC 5 cut(s) 45, 48, 487, 506, 540
Sau3AI GATC 1 cut(s) 271
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 2 cut(s) 177, 471
SexAI ACCWGGT 1 cut(s) 175
SfaNI GCATC 1 cut(s) 364
SmlI CTYRAG 2 cut(s) 360, 386
SmoI CTYRAG 2 cut(s) 360, 386
SphI GCATGC 1 cut(s) 546
Sse9I AATT 3 cut(s) 241, 316, 410
SsiI CCGC 7 cut(s) 18, 42, 45, 48, 311, 446, 540
StyD4I CCNGG 2 cut(s) 175, 469
TaaI ACNGT 1 cut(s) 496
TaiI ACGT 3 cut(s) 88, 210, 462
TaqI TCGA 5 cut(s) 129, 181, 264, 305, 438
TasI AATT 3 cut(s) 241, 316, 410
TauI GCSGC 3 cut(s) 47, 50, 542
TfiI GAWTC 2 cut(s) 325, 348
Tru1I TTAA 2 cut(s) 342, 387
Tru9I TTAA 2 cut(s) 342, 387
TseFI GTSAC 1 cut(s) 81
TseI GCWGC 2 cut(s) 486, 505
Tsp45I GTSAC 1 cut(s) 81
TspDTI ATGAA 1 cut(s) 63
TspGWI ACGGA 3 cut(s) 51, 120, 431
Vha464I CTTAAG 1 cut(s) 386
XceI RCATGY 1 cut(s) 546
XmiI GTMKAC 1 cut(s) 181
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.