Rw7G004010

HNH nucleases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0010466
Physical Location & Seq
Reverse (-)
3370171 .. 3372409
2239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G004010.1

Sequence Viewer

Length: 447 bp
ATGGGTAGTTCCTCATCGCCTCATCGCTCTCATGGTGGCAGTAACAATAGTAGCAGCAACGGCGAAGATAGGCCGCGATTCTTCGACCCAAAAGCAAAGAGCAAGTGCTGGGCAAACGCCGCAATAGTGCCAGGTCGACACCCTGAGCGGTGGCGCAAAGACGCCGCCGGCAACATTGTCTGCAAGCGCTTCTGCAATTGCCAGGGCTGCCTCTGCTTCGAGTACGATCACATACTCCCCTTTTCTAAAGGTGGTGAATCCGTAGCGGAAAACTGTCAGATTCTACAAACAAGAGTGAACAGATTCAAATCAAACAAAGAGCAGGTCGACGAGAATCAATTGAAAGGCTACTCTTGTGATGTCAAGTTTACAGCAGATCTGCTGGTACTTGAAGAATGTGTTTCCTTCATCCACTCGATGCATTCCTCTGTAGTCCCAGAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH

Protein Analysis

148

Amino Acids

16.47

Weight (kDa)

8.09

Isoelectric Point (pI)

61.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccBSI CCGCTC 1 cut(s) 148
AccI GTMKAC 2 cut(s) 136, 327
AccII CGCG 1 cut(s) 76
AciI CCGC 5 cut(s) 74, 120, 148, 165, 266
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 2 cut(s) 224, 387
AfeI AGCGCT 1 cut(s) 188
AgsI TTSAA 3 cut(s) 307, 343, 392
AjnI CCWGG 2 cut(s) 130, 201
Aor51HI AGCGCT 1 cut(s) 188
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 2 cut(s) 54, 207
ArsI GACNNNNNNTTYG 2 cut(s) 309, 341
Asp700I GAANNNNTTC 1 cut(s) 302
AspLEI GCGC 2 cut(s) 156, 189
AsuHPI GGTGA 1 cut(s) 266
BbvI GCAGC 2 cut(s) 66, 194
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 2 cut(s) 132, 203
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 313
BglII AGATCT 1 cut(s) 376
BisI GCNGC 5 cut(s) 55, 74, 120, 165, 208
BlsI GCNGC 5 cut(s) 56, 75, 121, 166, 209
Bme1390I CCNGG 2 cut(s) 132, 203
BmrFI CCNGG 2 cut(s) 132, 203
BmsI GCATC 1 cut(s) 408
Bpu10I CCTNAGC 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 307
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 202
Bse118I RCCGGY 1 cut(s) 167
Bse8I GATNNNNATC 1 cut(s) 307
BseBI CCWGG 2 cut(s) 132, 203
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 1 cut(s) 408
BseJI GATNNNNATC 1 cut(s) 307
BseMII CTCAG 1 cut(s) 135
BseXI GCAGC 2 cut(s) 66, 194
BseYI CCCAGC 1 cut(s) 108
Bsh1236I CGCG 1 cut(s) 76
BshFI GGCC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 419
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 1 cut(s) 73
Bsp143I GATC 2 cut(s) 226, 376
BspACI CCGC 5 cut(s) 74, 120, 148, 165, 266
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 136
BspFNI CGCG 1 cut(s) 76
BspMI ACCTGC 1 cut(s) 313
BsrBI CCGCTC 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 2 cut(s) 226, 376
BssNI GRCGYC 1 cut(s) 162
Bst2UI CCWGG 2 cut(s) 132, 203
Bst4CI ACNGT 1 cut(s) 275
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 2 cut(s) 169, 185
BstDEI CTNAG 1 cut(s) 144
BstF5I GGATG 1 cut(s) 408
BstFNI CGCG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 2 cut(s) 156, 189
BstKTI GATC 2 cut(s) 229, 379
BstMBI GATC 2 cut(s) 226, 376
BstMWI GCNNNNNNNGC 4 cut(s) 60, 119, 207, 213
BstNI CCWGG 2 cut(s) 132, 203
BstSCI CCNGG 2 cut(s) 130, 201
BstSFI CTRYAG 1 cut(s) 429
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 2 cut(s) 66, 194
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 1 cut(s) 73
BtgZI GCGATG 1 cut(s) 8
BtsCI GGATG 1 cut(s) 408
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 2 cut(s) 169, 185
CfoI GCGC 2 cut(s) 156, 189
Cfr10I RCCGGY 1 cut(s) 167
CseI GACGC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 223, 386
CviAII CATG 1 cut(s) 32
CviJI RGCY 3 cut(s) 73, 207, 348
CviKI_1 RGCY 3 cut(s) 73, 207, 348
CviQI GTAC 2 cut(s) 223, 386
DdeI CTNAG 1 cut(s) 144
DpnI GATC 2 cut(s) 228, 378
DpnII GATC 2 cut(s) 226, 376
Eco47III AGCGCT 1 cut(s) 188
EcoRII CCWGG 2 cut(s) 130, 201
EcoT22I ATGCAT 1 cut(s) 423
FaeI CATG 1 cut(s) 35
FaiI YATR 2 cut(s) 33, 233
FaqI GGGAC 1 cut(s) 419
FatI CATG 1 cut(s) 31
FblI GTMKAC 2 cut(s) 136, 327
Fnu4HI GCNGC 5 cut(s) 55, 74, 120, 165, 208
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 5 cut(s) 55, 74, 120, 165, 208
GlaI GCGC 2 cut(s) 155, 188
GluI GCNGC 5 cut(s) 55, 74, 120, 165, 208
GsaI CCCAGC 1 cut(s) 112
HaeII RGCGCY 1 cut(s) 190
HaeIII GGCC 1 cut(s) 73
HapII CCGG 1 cut(s) 168
HgaI GACGC 1 cut(s) 170
HhaI GCGC 2 cut(s) 156, 189
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 1 cut(s) 35
Hin6I GCGC 2 cut(s) 154, 187
HinP1I GCGC 2 cut(s) 154, 187
HincII GTYRAC 2 cut(s) 137, 328
HindII GTYRAC 2 cut(s) 137, 328
HinfI GANTC 5 cut(s) 78, 257, 280, 303, 334
HpaII CCGG 1 cut(s) 168
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 4 cut(s) 137, 298, 328, 369
Hpy188I TCNGA 1 cut(s) 279
Hpy8I GTNNAC 4 cut(s) 137, 298, 328, 369
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 2 cut(s) 415, 434
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4V TGCA 3 cut(s) 183, 195, 421
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 119, 207, 213
HpyF3I CTNAG 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 1 cut(s) 35
HspAI GCGC 2 cut(s) 154, 187
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 226, 376
LpnPI CCDG 9 cut(s) 94, 117, 144, 156, 181, 188, 215, 308, 368
Lsp1109I GCAGC 2 cut(s) 66, 194
LweI GCATC 1 cut(s) 408
MaeIII GTNAC 1 cut(s) 41
MalI GATC 2 cut(s) 228, 378
MbiI CCGCTC 1 cut(s) 148
MboI GATC 2 cut(s) 226, 376
MboII GAAGA 3 cut(s) 73, 77, 404
MfeI CAATTG 2 cut(s) 196, 338
MflI RGATCY 1 cut(s) 376
MluCI AATT 2 cut(s) 196, 338
MnlI CCTC 4 cut(s) 22, 30, 221, 436
Mph1103I ATGCAT 1 cut(s) 423
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 302
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 2 cut(s) 132, 203
MunI CAATTG 2 cut(s) 196, 338
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 2 cut(s) 132, 203
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 4 cut(s) 60, 119, 207, 213
NaeI GCCGGC 1 cut(s) 169
NdeII GATC 2 cut(s) 226, 376
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 423
PctI GAATGC 1 cut(s) 421
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 5 cut(s) 78, 257, 280, 303, 334
PkrI GCNGC 5 cut(s) 56, 75, 121, 166, 209
Psp6I CCWGG 2 cut(s) 130, 201
PspFI CCCAGC 1 cut(s) 108
PspGI CCWGG 2 cut(s) 130, 201
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 2 cut(s) 224, 387
RsaNI GTAC 2 cut(s) 223, 386
SalI GTCGAC 2 cut(s) 135, 326
SatI GCNGC 5 cut(s) 55, 74, 120, 165, 208
Sau3AI GATC 2 cut(s) 226, 376
ScrFI CCNGG 2 cut(s) 132, 203
SetI ASST 3 cut(s) 136, 253, 327
SfaNI GCATC 1 cut(s) 408
SfcI CTRYAG 1 cut(s) 429
Sse9I AATT 2 cut(s) 196, 338
SsiI CCGC 5 cut(s) 74, 120, 148, 165, 266
StyD4I CCNGG 2 cut(s) 130, 201
TaaI ACNGT 1 cut(s) 275
TaqI TCGA 5 cut(s) 84, 136, 219, 327, 416
TasI AATT 2 cut(s) 196, 338
TauI GCSGC 3 cut(s) 76, 122, 167
TfiI GAWTC 5 cut(s) 78, 257, 280, 303, 334
TseI GCWGC 2 cut(s) 54, 207
TspDTI ATGAA 1 cut(s) 397
TspGWI ACGGA 1 cut(s) 250
XmiI GTMKAC 2 cut(s) 136, 327
XmnI GAANNNNTTC 1 cut(s) 302
Zsp2I ATGCAT 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.