Rh7AG049100

HNH nucleases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0010466
Physical Location & Seq
Reverse (-)
3403401 .. 3404919
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG049100.1

Sequence Viewer

Length: 531 bp
ATGGGTAGTTCCTCATCGCCTCATCGCTCTCATGGTGGCAGTAACAATAGTAGCAGCAACGGCGAAGATAGGCCGCGATTCTTCGACCCAAAAGCAAAGAGTAAGTGCTGGGCAAACGCCGCAATAGTGCCAGGTCGACACCCTGAGCGGTGGCGCAAAGACGCCGCCGGCAACATTGTCTGCAAGCGCTTCTGCAATTGCCAGGGCTGCCTCTGCTTCGAGTACGATCACATACTCCCCTTTTCTAAAGGTGGTGAATCCGTAGCCGAAAACTGTCAGATTCTACAAACAAGAGTGAACAGATTCAAATCAAACAAAGAGCAGGTCGACGAGAATCAATTGAAAGGCTACTCTTGTGATGTCAAGTTTACAGATAAGGAGCTTGACATAATTGAAATGGCTGTTTACGGTGATGTGGTCCGGCCAGGAAACCAGTGCCGATGCAGAACTGTAGCCGAGATGCTGGGCCAGGTCAAGTCAAAAGACAAAACTGCCGCTTGCAAGTTGCCACATAGCAGTGAGGCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH

Protein Analysis

176

Amino Acids

19.57

Weight (kDa)

8.76

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccBSI CCGCTC 1 cut(s) 148
AccI GTMKAC 2 cut(s) 136, 327
AccII CGCG 1 cut(s) 76
AciI CCGC 5 cut(s) 74, 120, 148, 165, 495
AcoI YGGCCR 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 1 cut(s) 224
AfeI AGCGCT 1 cut(s) 188
AgsI TTSAA 3 cut(s) 307, 343, 395
AjnI CCWGG 4 cut(s) 130, 201, 424, 468
AluBI AGCT 1 cut(s) 382
AluI AGCT 1 cut(s) 382
Aor51HI AGCGCT 1 cut(s) 188
AoxI GGCC 3 cut(s) 71, 422, 466
ApeKI GCWGC 2 cut(s) 54, 207
ArsI GACNNNNNNTTYG 2 cut(s) 309, 341
Asp700I GAANNNNTTC 1 cut(s) 302
AspLEI GCGC 2 cut(s) 156, 189
AspS9I GGNCC 2 cut(s) 418, 466
AsuHPI GGTGA 2 cut(s) 266, 422
AvaII GGWCC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 66, 194
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 4 cut(s) 132, 203, 426, 470
BfmI CTRYAG 2 cut(s) 450, 527
BfoI RGCGCY 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 313
BisI GCNGC 6 cut(s) 55, 74, 120, 165, 208, 495
BlsI GCNGC 6 cut(s) 56, 75, 121, 166, 209, 496
Bme1390I CCNGG 4 cut(s) 132, 203, 426, 470
Bme18I GGWCC 1 cut(s) 418
BmgT120I GGNCC 2 cut(s) 418, 466
BmrFI CCNGG 4 cut(s) 132, 203, 426, 470
BmsI GCATC 2 cut(s) 431, 450
Bpu10I CCTNAGC 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 307
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 202
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 433
Bse8I GATNNNNATC 1 cut(s) 307
BseBI CCWGG 4 cut(s) 132, 203, 426, 470
BseDI CCNNGG 1 cut(s) 202
BseJI GATNNNNATC 1 cut(s) 307
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 1 cut(s) 433
BseXI GCAGC 2 cut(s) 66, 194
BseYI CCCAGC 2 cut(s) 108, 463
Bsh1236I CGCG 1 cut(s) 76
BshFI GGCC 3 cut(s) 73, 424, 468
BsiSI CCGG 2 cut(s) 168, 421
BsnI GGCC 3 cut(s) 73, 424, 468
Bsp143I GATC 1 cut(s) 226
BspACI CCGC 5 cut(s) 74, 120, 148, 165, 495
BspANI GGCC 3 cut(s) 73, 424, 468
BspCNI CTCAG 1 cut(s) 136
BspFNI CGCG 1 cut(s) 76
BspMI ACCTGC 1 cut(s) 313
BsrBI CCGCTC 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 1 cut(s) 433
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 1 cut(s) 226
BssNI GRCGYC 1 cut(s) 162
Bst2UI CCWGG 4 cut(s) 132, 203, 426, 470
Bst4CI ACNGT 3 cut(s) 275, 410, 451
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 3 cut(s) 169, 185, 499
BstDEI CTNAG 1 cut(s) 144
BstFNI CGCG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 2 cut(s) 156, 189
BstKTI GATC 1 cut(s) 229
BstMBI GATC 1 cut(s) 226
BstMWI GCNNNNNNNGC 4 cut(s) 60, 119, 207, 213
BstNI CCWGG 4 cut(s) 132, 203, 426, 470
BstSCI CCNGG 4 cut(s) 130, 201, 424, 468
BstSFI CTRYAG 2 cut(s) 450, 527
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 2 cut(s) 66, 194
BsuRI GGCC 3 cut(s) 73, 424, 468
BtgZI GCGATG 1 cut(s) 8
BtsI GCAGTG 1 cut(s) 523
BtsIMutI CAGTG 2 cut(s) 440, 523
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 3 cut(s) 169, 185, 499
CfoI GCGC 2 cut(s) 156, 189
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 418, 466
CseI GACGC 1 cut(s) 170
Csp6I GTAC 1 cut(s) 223
CviAII CATG 1 cut(s) 32
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 1 cut(s) 144
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 422
Eco47I GGWCC 1 cut(s) 418
Eco47III AGCGCT 1 cut(s) 188
EcoRII CCWGG 4 cut(s) 130, 201, 424, 468
FaeI CATG 1 cut(s) 35
FaiI YATR 5 cut(s) 33, 233, 389, 513, 529
FatI CATG 1 cut(s) 31
FblI GTMKAC 2 cut(s) 136, 327
Fnu4HI GCNGC 6 cut(s) 55, 74, 120, 165, 208, 495
Fsp4HI GCNGC 6 cut(s) 55, 74, 120, 165, 208, 495
GlaI GCGC 2 cut(s) 155, 188
GluI GCNGC 6 cut(s) 55, 74, 120, 165, 208, 495
GsaI CCCAGC 2 cut(s) 112, 467
HaeII RGCGCY 1 cut(s) 190
HaeIII GGCC 3 cut(s) 73, 424, 468
HapII CCGG 2 cut(s) 168, 421
HgaI GACGC 1 cut(s) 170
HhaI GCGC 2 cut(s) 156, 189
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 1 cut(s) 35
Hin6I GCGC 2 cut(s) 154, 187
HinP1I GCGC 2 cut(s) 154, 187
HincII GTYRAC 2 cut(s) 137, 328
HindII GTYRAC 2 cut(s) 137, 328
HinfI GANTC 5 cut(s) 78, 257, 280, 303, 334
HpaII CCGG 2 cut(s) 168, 421
HphI GGTGA 2 cut(s) 266, 422
Hpy166II GTNNAC 5 cut(s) 137, 298, 328, 369, 406
Hpy188I TCNGA 1 cut(s) 279
Hpy8I GTNNAC 5 cut(s) 137, 298, 328, 369, 406
Hpy99I CGWCG 1 cut(s) 332
HpyCH4III ACNGT 3 cut(s) 275, 410, 451
HpyCH4V TGCA 4 cut(s) 183, 195, 444, 501
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 119, 207, 213
HpyF3I CTNAG 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 1 cut(s) 35
HspAI GCGC 2 cut(s) 154, 187
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 1 cut(s) 226
LmnI GCTCC 1 cut(s) 379
Lsp1109I GCAGC 2 cut(s) 66, 194
LweI GCATC 2 cut(s) 431, 450
MaeIII GTNAC 1 cut(s) 41
MalI GATC 1 cut(s) 228
MbiI CCGCTC 1 cut(s) 148
MboI GATC 1 cut(s) 226
MboII GAAGA 2 cut(s) 73, 77
MfeI CAATTG 2 cut(s) 196, 338
MluCI AATT 3 cut(s) 196, 338, 390
MnlI CCTC 4 cut(s) 22, 30, 221, 514
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 302
MslI CAYNNNNRTG 1 cut(s) 516
MspI CCGG 2 cut(s) 168, 421
MspR9I CCNGG 4 cut(s) 132, 203, 426, 470
MunI CAATTG 2 cut(s) 196, 338
MvaI CCWGG 4 cut(s) 132, 203, 426, 470
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 4 cut(s) 60, 119, 207, 213
NaeI GCCGGC 1 cut(s) 169
NdeII GATC 1 cut(s) 226
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 1 cut(s) 35
NmeAIII GCCGAG 1 cut(s) 481
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 5 cut(s) 78, 257, 280, 303, 334
PkrI GCNGC 6 cut(s) 56, 75, 121, 166, 209, 496
Psp6I CCWGG 4 cut(s) 130, 201, 424, 468
PspFI CCCAGC 2 cut(s) 108, 463
PspGI CCWGG 4 cut(s) 130, 201, 424, 468
PspPI GGNCC 2 cut(s) 418, 466
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 1 cut(s) 516
SalI GTCGAC 2 cut(s) 135, 326
SatI GCNGC 6 cut(s) 55, 74, 120, 165, 208, 495
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 2 cut(s) 418, 466
ScrFI CCNGG 4 cut(s) 132, 203, 426, 470
SetI ASST 5 cut(s) 136, 253, 327, 384, 474
SfaNI GCATC 2 cut(s) 431, 450
SfcI CTRYAG 2 cut(s) 450, 527
SinI GGWCC 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 516
Sse9I AATT 3 cut(s) 196, 338, 390
SsiI CCGC 5 cut(s) 74, 120, 148, 165, 495
StyD4I CCNGG 4 cut(s) 130, 201, 424, 468
TaaI ACNGT 3 cut(s) 275, 410, 451
TaqI TCGA 4 cut(s) 84, 136, 219, 327
TasI AATT 3 cut(s) 196, 338, 390
TauI GCSGC 4 cut(s) 76, 122, 167, 497
TfiI GAWTC 5 cut(s) 78, 257, 280, 303, 334
TscAI CASTG 2 cut(s) 440, 523
TseI GCWGC 2 cut(s) 54, 207
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 2 cut(s) 440, 523
VpaK11BI GGWCC 1 cut(s) 418
XmiI GTMKAC 2 cut(s) 136, 327
XmnI GAANNNNTTC 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.