pycom14g17030

HNH nucleases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010466
Physical Location & Seq
Reverse (-)
19125525 .. 19127807
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g17030.1

Sequence Viewer

Length: 477 bp
ATGAGTTCTTCGCCGAAGAGCAAGGGTGACGAAAGACCACGGTTCTTCGATTCAAAAACGAAGAGCAAGTGCTGGGCAAACGCGGATGTGGTGCCGGGTCGACACCCTGAGCGGTGGCGGCAAGACGCCGCCGGCAACGTCGTCTGTAAGCGCTTCTGCAACTGCCAGGGCTGCCTCTGCTTCGAGTACGATCACATCGTTCCCTTCTCCAAAGGTGGTGAATCCACAGAAGAAAATTGTCAGATTCTTCAAACAAGGGTAAACAGATTCAAATCAGACAAAGACAGAATCGATACAAATATATTGAAAGGTTACTCTTGTGATGTCAAGTTTACAGATAAGGAGCTTGACGTAATCGAAATGGCCGTATATGGTGACGTGATCCGCCCAGGAAACCAATGCCGATGCAGAACTATAGCCGAGATGCTTGGACAGCACAAGTCGAAAGACCAAACAGCTGCTTGCAAGTTGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH

Protein Analysis

159

Amino Acids

17.95

Weight (kDa)

8.63

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 91
AccBSI CCGCTC 1 cut(s) 112
AccI GTMKAC 1 cut(s) 100
AccII CGCG 1 cut(s) 83
AciI CCGC 5 cut(s) 83, 112, 118, 129, 385
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 1 cut(s) 363
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 1 cut(s) 188
AfeI AGCGCT 1 cut(s) 152
AgsI TTSAA 4 cut(s) 54, 251, 271, 307
AjiI CACGTC 1 cut(s) 379
AjnI CCWGG 2 cut(s) 165, 388
AluBI AGCT 2 cut(s) 346, 458
AluI AGCT 2 cut(s) 346, 458
AlwI GGATC 1 cut(s) 376
Aor51HI AGCGCT 1 cut(s) 152
AoxI GGCC 1 cut(s) 363
ApeKI GCWGC 2 cut(s) 171, 458
AspLEI GCGC 1 cut(s) 153
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 3 cut(s) 38, 230, 386
BanI GGYRCC 1 cut(s) 91
BbvI GCAGC 2 cut(s) 158, 445
BceAI ACGGC 1 cut(s) 350
BciT130I CCWGG 2 cut(s) 167, 390
BcnI CCSGG 1 cut(s) 96
BfmI CTRYAG 1 cut(s) 414
BfoI RGCGCY 1 cut(s) 154
BisI GCNGC 4 cut(s) 119, 129, 172, 459
BlsI GCNGC 4 cut(s) 120, 130, 173, 460
Bme1390I CCNGG 3 cut(s) 96, 167, 390
BmgBI CACGTC 1 cut(s) 379
BmiI GGNNCC 1 cut(s) 93
BmrFI CCNGG 3 cut(s) 96, 167, 390
BmsI GCATC 2 cut(s) 395, 414
Bpu10I CCTNAGC 1 cut(s) 108
BpuMI CCSGG 1 cut(s) 96
Bsa29I ATCGAT 1 cut(s) 291
BsaBI GATNNNNATC 1 cut(s) 271
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 38, 166, 388
Bse118I RCCGGY 1 cut(s) 131
Bse8I GATNNNNATC 1 cut(s) 271
BseBI CCWGG 2 cut(s) 167, 390
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 3 cut(s) 38, 166, 388
BseGI GGATG 1 cut(s) 91
BseJI GATNNNNATC 1 cut(s) 271
BseMII CTCAG 1 cut(s) 99
BseXI GCAGC 2 cut(s) 158, 445
BseYI CCCAGC 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 1 cut(s) 365
BshNI GGYRCC 1 cut(s) 91
BshVI ATCGAT 1 cut(s) 291
BsiSI CCGG 2 cut(s) 95, 132
BsnI GGCC 1 cut(s) 365
Bsp143I GATC 2 cut(s) 190, 381
BspACI CCGC 5 cut(s) 83, 112, 118, 129, 385
BspANI GGCC 1 cut(s) 365
BspCNI CTCAG 1 cut(s) 100
BspDI ATCGAT 1 cut(s) 291
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 1 cut(s) 93
BspPI GGATC 1 cut(s) 376
BspQI GCTCTTC 2 cut(s) 11, 56
BspT107I GGYRCC 1 cut(s) 91
BsrBI CCGCTC 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 131
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 38, 166, 388
BssMI GATC 2 cut(s) 190, 381
BssNI GRCGYC 1 cut(s) 126
Bst2UI CCWGG 2 cut(s) 167, 390
Bst4CI ACNGT 1 cut(s) 42
Bst6I CTCTTC 2 cut(s) 11, 56
BstACI GRCGYC 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 133, 463
BstDEI CTNAG 1 cut(s) 108
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 1 cut(s) 91
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 2 cut(s) 193, 384
BstMBI GATC 2 cut(s) 190, 381
BstMWI GCNNNNNNNGC 4 cut(s) 118, 171, 177, 433
BstNI CCWGG 2 cut(s) 167, 390
BstSCI CCNGG 3 cut(s) 94, 165, 388
BstSFI CTRYAG 1 cut(s) 414
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 2 cut(s) 158, 445
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 1 cut(s) 365
BsuTUI ATCGAT 1 cut(s) 291
BtgI CCRYGG 1 cut(s) 38
BtrI CACGTC 1 cut(s) 379
BtsCI GGATG 1 cut(s) 91
Cac8I GCNNGC 2 cut(s) 133, 463
CfoI GCGC 1 cut(s) 153
Cfr10I RCCGGY 1 cut(s) 131
ClaI ATCGAT 1 cut(s) 291
CseI GACGC 1 cut(s) 134
Csp6I GTAC 1 cut(s) 187
CviJI RGCY 5 cut(s) 171, 346, 365, 419, 458
CviKI_1 RGCY 5 cut(s) 171, 346, 365, 419, 458
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 1 cut(s) 108
DpnI GATC 2 cut(s) 192, 383
DpnII GATC 2 cut(s) 190, 381
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 2 cut(s) 11, 56
EarI CTCTTC 2 cut(s) 11, 56
EciI GGCGGA 1 cut(s) 374
Eco47III AGCGCT 1 cut(s) 152
EcoRII CCWGG 2 cut(s) 165, 388
FaiI YATR 5 cut(s) 302, 370, 372, 416, 475
FblI GTMKAC 1 cut(s) 100
Fnu4HI GCNGC 4 cut(s) 119, 129, 172, 459
FokI GGATG 1 cut(s) 98
Fsp4HI GCNGC 4 cut(s) 119, 129, 172, 459
GlaI GCGC 1 cut(s) 152
GluI GCNGC 4 cut(s) 119, 129, 172, 459
GsaI CCCAGC 1 cut(s) 76
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 1 cut(s) 365
HapII CCGG 2 cut(s) 95, 132
HgaI GACGC 1 cut(s) 134
HhaI GCGC 1 cut(s) 153
Hin1I GRCGYC 1 cut(s) 126
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HincII GTYRAC 1 cut(s) 101
HindII GTYRAC 1 cut(s) 101
HinfI GANTC 5 cut(s) 50, 221, 244, 267, 288
HpaII CCGG 2 cut(s) 95, 132
HphI GGTGA 3 cut(s) 38, 230, 386
Hpy166II GTNNAC 3 cut(s) 101, 262, 333
Hpy188I TCNGA 2 cut(s) 243, 277
Hpy8I GTNNAC 3 cut(s) 101, 262, 333
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4IV ACGT 3 cut(s) 138, 351, 378
HpyCH4V TGCA 3 cut(s) 159, 408, 465
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 171, 177, 433
HpyF3I CTNAG 1 cut(s) 108
HpySE526I ACGT 3 cut(s) 138, 351, 378
Hsp92I GRCGYC 1 cut(s) 126
HspAI GCGC 1 cut(s) 151
KroI GCCGGC 1 cut(s) 131
KroNI GCCGGC 1 cut(s) 133
Kzo9I GATC 2 cut(s) 190, 381
LguI GCTCTTC 2 cut(s) 11, 56
LmnI GCTCC 1 cut(s) 343
LpnPI CCDG 8 cut(s) 58, 108, 120, 145, 152, 179, 375, 402
Lsp1109I GCAGC 2 cut(s) 158, 445
LweI GCATC 2 cut(s) 395, 414
MaeII ACGT 3 cut(s) 138, 351, 378
MaeIII GTNAC 3 cut(s) 26, 311, 374
MalI GATC 2 cut(s) 192, 383
MbiI CCGCTC 1 cut(s) 112
MboI GATC 2 cut(s) 190, 381
MboII GAAGA 5 cut(s) 28, 37, 73, 239, 242
MluCI AATT 1 cut(s) 235
MnlI CCTC 1 cut(s) 185
MroNI GCCGGC 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 2 cut(s) 95, 132
MspR9I CCNGG 3 cut(s) 96, 167, 390
MvaI CCWGG 2 cut(s) 167, 390
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 4 cut(s) 118, 171, 177, 433
NaeI GCCGGC 1 cut(s) 133
NciI CCSGG 1 cut(s) 96
NdeII GATC 2 cut(s) 190, 381
NgoMIV GCCGGC 1 cut(s) 131
NlaIV GGNNCC 1 cut(s) 93
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 2 cut(s) 26, 374
PciSI GCTCTTC 2 cut(s) 11, 56
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 363
PdiI GCCGGC 1 cut(s) 133
PfeI GAWTC 5 cut(s) 50, 221, 244, 267, 288
PkrI GCNGC 4 cut(s) 120, 130, 173, 460
Psp6I CCWGG 2 cut(s) 165, 388
PspFI CCCAGC 1 cut(s) 72
PspGI CCWGG 2 cut(s) 165, 388
PspN4I GGNNCC 1 cut(s) 93
PvuII CAGCTG 1 cut(s) 458
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SalI GTCGAC 1 cut(s) 99
SapI GCTCTTC 2 cut(s) 11, 56
SatI GCNGC 4 cut(s) 119, 129, 172, 459
Sau3AI GATC 2 cut(s) 190, 381
ScrFI CCNGG 3 cut(s) 96, 167, 390
SetI ASST 7 cut(s) 141, 217, 313, 348, 354, 381, 460
SfaNI GCATC 2 cut(s) 395, 414
SfcI CTRYAG 1 cut(s) 414
Sse9I AATT 1 cut(s) 235
SsiI CCGC 5 cut(s) 83, 112, 118, 129, 385
StyD4I CCNGG 3 cut(s) 94, 165, 388
TaaI ACNGT 1 cut(s) 42
TaiI ACGT 3 cut(s) 141, 354, 381
TaqI TCGA 6 cut(s) 48, 100, 183, 291, 357, 443
TasI AATT 1 cut(s) 235
TauI GCSGC 2 cut(s) 121, 131
TfiI GAWTC 5 cut(s) 50, 221, 244, 267, 288
TseFI GTSAC 2 cut(s) 26, 374
TseI GCWGC 2 cut(s) 171, 458
Tsp45I GTSAC 2 cut(s) 26, 374
XmiI GTMKAC 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.