Rmu_sc0033556.1_g000001

HNH nucleases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0033556.1
OG0010466
Physical Location & Seq
Reverse (-)
1 .. 1228
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0033556.1_g000001.1.cds

Sequence Viewer

Length: 373 bp
atgggtagttcctcatcgcctcatcgctctcatggtggcagtaacaatagtagcagcaacggcgaagataggccgcgattcttcgacccaaaagcaaagagtaagtgctgggcaaacgccgcaatagtgccaggtcgacaccctgagcggtggcgcaaagacgccgccggcaacattgtctgcaagcgcttctgcaattgccagggctgcctctgcttcgagtacgatcacatactccccttttctaaaggtggtgaatccgtagccgaaaactgtcagattctacaaacaagagtgaacagattcaaatcaaacaaagagcaggtcgacgagaatcaattgaaaggctactcttgtgatgtcaagtttacag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH

Protein Analysis

124

Amino Acids

13.89

Weight (kDa)

8.99

Isoelectric Point (pI)

56.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccBSI CCGCTC 1 cut(s) 148
AccI GTMKAC 2 cut(s) 136, 327
AccII CGCG 1 cut(s) 76
AciI CCGC 4 cut(s) 74, 120, 148, 165
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 1 cut(s) 224
AfeI AGCGCT 1 cut(s) 188
AgsI TTSAA 2 cut(s) 307, 343
AjnI CCWGG 2 cut(s) 130, 201
Aor51HI AGCGCT 1 cut(s) 188
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 2 cut(s) 54, 207
ArsI GACNNNNNNTTYG 2 cut(s) 309, 341
Asp700I GAANNNNTTC 1 cut(s) 302
AspLEI GCGC 2 cut(s) 156, 189
AsuHPI GGTGA 1 cut(s) 266
BbvI GCAGC 2 cut(s) 66, 194
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 2 cut(s) 132, 203
BfoI RGCGCY 1 cut(s) 190
BfuAI ACCTGC 1 cut(s) 313
BisI GCNGC 5 cut(s) 55, 74, 120, 165, 208
BlsI GCNGC 5 cut(s) 56, 75, 121, 166, 209
Bme1390I CCNGG 2 cut(s) 132, 203
BmrFI CCNGG 2 cut(s) 132, 203
Bpu10I CCTNAGC 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 307
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 202
Bse118I RCCGGY 1 cut(s) 167
Bse8I GATNNNNATC 1 cut(s) 307
BseBI CCWGG 2 cut(s) 132, 203
BseDI CCNNGG 1 cut(s) 202
BseJI GATNNNNATC 1 cut(s) 307
BseMII CTCAG 1 cut(s) 135
BseXI GCAGC 2 cut(s) 66, 194
BseYI CCCAGC 1 cut(s) 108
Bsh1236I CGCG 1 cut(s) 76
BshFI GGCC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 168
BsnI GGCC 1 cut(s) 73
Bsp143I GATC 1 cut(s) 226
BspACI CCGC 4 cut(s) 74, 120, 148, 165
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 1 cut(s) 136
BspFNI CGCG 1 cut(s) 76
BspMI ACCTGC 1 cut(s) 313
BsrBI CCGCTC 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 1 cut(s) 226
BssNI GRCGYC 1 cut(s) 162
Bst2UI CCWGG 2 cut(s) 132, 203
Bst4CI ACNGT 1 cut(s) 275
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 2 cut(s) 169, 185
BstDEI CTNAG 1 cut(s) 144
BstFNI CGCG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 2 cut(s) 156, 189
BstKTI GATC 1 cut(s) 229
BstMBI GATC 1 cut(s) 226
BstMWI GCNNNNNNNGC 4 cut(s) 60, 119, 207, 213
BstNI CCWGG 2 cut(s) 132, 203
BstSCI CCNGG 2 cut(s) 130, 201
BstUI CGCG 1 cut(s) 76
BstV1I GCAGC 2 cut(s) 66, 194
BsuRI GGCC 1 cut(s) 73
BtgZI GCGATG 1 cut(s) 8
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 2 cut(s) 169, 185
CfoI GCGC 2 cut(s) 156, 189
Cfr10I RCCGGY 1 cut(s) 167
CseI GACGC 1 cut(s) 170
Csp6I GTAC 1 cut(s) 223
CviAII CATG 1 cut(s) 32
CviJI RGCY 4 cut(s) 73, 207, 266, 348
CviKI_1 RGCY 4 cut(s) 73, 207, 266, 348
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 1 cut(s) 144
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
Eco47III AGCGCT 1 cut(s) 188
EcoRII CCWGG 2 cut(s) 130, 201
FaeI CATG 1 cut(s) 35
FaiI YATR 2 cut(s) 33, 233
FatI CATG 1 cut(s) 31
FblI GTMKAC 2 cut(s) 136, 327
Fnu4HI GCNGC 5 cut(s) 55, 74, 120, 165, 208
Fsp4HI GCNGC 5 cut(s) 55, 74, 120, 165, 208
GlaI GCGC 2 cut(s) 155, 188
GluI GCNGC 5 cut(s) 55, 74, 120, 165, 208
GsaI CCCAGC 1 cut(s) 112
HaeII RGCGCY 1 cut(s) 190
HaeIII GGCC 1 cut(s) 73
HapII CCGG 1 cut(s) 168
HgaI GACGC 1 cut(s) 170
HhaI GCGC 2 cut(s) 156, 189
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 1 cut(s) 35
Hin6I GCGC 2 cut(s) 154, 187
HinP1I GCGC 2 cut(s) 154, 187
HincII GTYRAC 2 cut(s) 137, 328
HindII GTYRAC 2 cut(s) 137, 328
HinfI GANTC 5 cut(s) 78, 257, 280, 303, 334
HpaII CCGG 1 cut(s) 168
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 4 cut(s) 137, 298, 328, 369
Hpy188I TCNGA 1 cut(s) 279
Hpy8I GTNNAC 4 cut(s) 137, 298, 328, 369
Hpy99I CGWCG 1 cut(s) 332
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4V TGCA 2 cut(s) 183, 195
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 119, 207, 213
HpyF3I CTNAG 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 1 cut(s) 35
HspAI GCGC 2 cut(s) 154, 187
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 1 cut(s) 226
LpnPI CCDG 8 cut(s) 94, 117, 144, 156, 181, 188, 215, 308
Lsp1109I GCAGC 2 cut(s) 66, 194
MaeIII GTNAC 1 cut(s) 41
MalI GATC 1 cut(s) 228
MbiI CCGCTC 1 cut(s) 148
MboI GATC 1 cut(s) 226
MboII GAAGA 2 cut(s) 73, 77
MfeI CAATTG 2 cut(s) 196, 338
MluCI AATT 2 cut(s) 196, 338
MnlI CCTC 3 cut(s) 22, 30, 221
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 302
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 2 cut(s) 132, 203
MunI CAATTG 2 cut(s) 196, 338
MvaI CCWGG 2 cut(s) 132, 203
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 4 cut(s) 60, 119, 207, 213
NaeI GCCGGC 1 cut(s) 169
NdeII GATC 1 cut(s) 226
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 1 cut(s) 35
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 5 cut(s) 78, 257, 280, 303, 334
PkrI GCNGC 5 cut(s) 56, 75, 121, 166, 209
Psp6I CCWGG 2 cut(s) 130, 201
PspFI CCCAGC 1 cut(s) 108
PspGI CCWGG 2 cut(s) 130, 201
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SalI GTCGAC 2 cut(s) 135, 326
SatI GCNGC 5 cut(s) 55, 74, 120, 165, 208
Sau3AI GATC 1 cut(s) 226
ScrFI CCNGG 2 cut(s) 132, 203
SetI ASST 3 cut(s) 136, 253, 327
Sse9I AATT 2 cut(s) 196, 338
SsiI CCGC 4 cut(s) 74, 120, 148, 165
StyD4I CCNGG 2 cut(s) 130, 201
TaaI ACNGT 1 cut(s) 275
TaqI TCGA 4 cut(s) 84, 136, 219, 327
TasI AATT 2 cut(s) 196, 338
TauI GCSGC 3 cut(s) 76, 122, 167
TfiI GAWTC 5 cut(s) 78, 257, 280, 303, 334
TseI GCWGC 2 cut(s) 54, 207
TspGWI ACGGA 1 cut(s) 250
XmiI GTMKAC 2 cut(s) 136, 327
XmnI GAANNNNTTC 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.