AT1G21620

Arabidopsis Pumilio

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009111
Physical Location & Seq
Forward (+)
7579129 .. 7580171
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G21620.1

Sequence Viewer

Length: 927 bp
ATGGCACACCAGCTCCGTTTTGCCGCCGCAAGAGAACCCATCTCAGAAGAGTATCCGGCGGTTGCTCTTCCTCATCCACGTTGCATCTCCGTCGCGTTTCCTCCTCCGAGATTCACACCATCTCCGTCGCCTTTAACTAATTACTCATTCTTGGCCGCATTAACTCCTCAAGAACTGGAGATGCGTTTGCAAATTGCAATGGATGTCCAGATGATCTCGAAGTTAGACCAAAGAAAGCTTCAAACGATGGCGTCATTGCTGACATCAGATCCCGATTACTTCTTGATGATCGCAAGAAACATGAACGGCTCCAAACGCATTCAGAAACTTCTCGGAAAAACAGATGACGTGGACGCTTTATTCGCCGCCGCTATCTTGCGCCGCTTCCTCCACATCATCACCGACAAGTACGCGTCGTACGTGGTGAGACGGGGGATGACAGTTTTCGATAAGAAGAAGAAAAAAGCAATGTACGAGCACATTCTCCACTACGCGAGCCACATAGCGCGTGATAAACACGCTCTTGTACTAAGCAACGATGCTTATGGGAACTTTGTGATACAACGCGTGCTTAAACTGAATGACTTGCGTAGCAAGAACAACATCGTCGTTAGTCTACGTGGCCACTTTGTTGATCTCTCGTTTCAGAAATATGGAAGCTACGTTGTGGATGTACTTTTGGAGACGAAGGAGTCAATGGTTGTGGTGGTGGAGGAGCTTATGGAATGTGAAGGAGATATGTTGATGAGGTTGGCGAGGAATGAGTATGGGAATTTCTTGGTGTGCAAGGCACTGAGAGTCACGCAGAAGGAGATGGTTAGGACTGATCTGTTTTGGGGTTTGGTGCATAAGCTCAAGCCTTTTCACAATCTCTTGCGTTGGTCTCGTGGAAAGAACATTGCATCCATCTTAAACTCGATTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

308

Amino Acids

35.41

Weight (kDa)

9.68

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01410 AT1G21620 AT1G35850 AT1G35850 AT5G59280 AT5G60110 AT5G60180 AT5G64490
fragaria_vesca FvH4_3g00680
malus_domestica MD05G1360200.v1.1
prunus_persica Prupe.4G007700_v2.0.a1
pyrus_communis pycom05g32520
rosa_chinensis RchiOBHm_Chr5g0000911
rosa_laevigata RLG00000030906
rosa_roxburghii Rroxscaffold_1G00075340
rosa_rugosa Rorug04G0387800 Rorug04G0387900.1
rosa_samantha Rh5AG007400 Rh5BG009500 Rh5CG008100 Rh5DG008100
rosa_wichuraiana Rw5G000770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 691
AccI GTMKAC 1 cut(s) 616
AccII CGCG 5 cut(s) 95, 413, 494, 508, 567
AciI CCGC 7 cut(s) 24, 27, 59, 156, 366, 369, 382
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 2 cut(s) 153, 622
AcsI RAATTY 1 cut(s) 772
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 5 cut(s) 410, 419, 473, 528, 675
AflIII ACRYGT 2 cut(s) 411, 565
AgsI TTSAA 1 cut(s) 242
AjiI CACGTC 1 cut(s) 349
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AloI GAACNNNNNNTCC 2 cut(s) 887, 919
AluBI AGCT 5 cut(s) 13, 238, 660, 718, 853
AluI AGCT 5 cut(s) 13, 238, 660, 718, 853
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 3 cut(s) 421, 677, 888
AlwI GGATC 1 cut(s) 263
AoxI GGCC 2 cut(s) 153, 622
ApoI RAATTY 1 cut(s) 772
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AspLEI GCGC 2 cut(s) 381, 508
AsuHPI GGTGA 2 cut(s) 391, 436
BalI TGGCCA 1 cut(s) 624
BauI CACGAG 1 cut(s) 885
Bbv12I GWGCWC 1 cut(s) 480
BccI CCATC 5 cut(s) 47, 127, 241, 808, 914
BceAI ACGGC 1 cut(s) 322
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciVI GTATCC 1 cut(s) 63
BcoDI GTCTC 3 cut(s) 421, 677, 888
BfuI GTATCC 1 cut(s) 63
BisI GCNGC 6 cut(s) 24, 27, 156, 366, 369, 382
BlsI GCNGC 6 cut(s) 25, 28, 157, 367, 370, 383
BmgBI CACGTC 1 cut(s) 349
BmiI GGNNCC 1 cut(s) 310
BmsI GCATC 4 cut(s) 93, 171, 529, 911
BpmI CTGGAG 1 cut(s) 197
BpuEI CTTGAG 2 cut(s) 153, 839
BsaAI YACGTR 2 cut(s) 421, 620
BsaHI GRCGYC 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 888
BsaXI ACNNNNNCTCC 3 cut(s) 27, 106, 136
Bse1I ACTGG 1 cut(s) 180
Bse3DI GCAATG 4 cut(s) 204, 254, 474, 897
BseGI GGATG 5 cut(s) 73, 208, 441, 676, 902
BseMI GCAATG 4 cut(s) 204, 254, 474, 897
BseMII CTCAG 2 cut(s) 57, 785
BseNI ACTGG 1 cut(s) 180
BseRI GAGGAG 3 cut(s) 93, 156, 728
Bsh1236I CGCG 5 cut(s) 95, 413, 494, 508, 567
BshFI GGCC 2 cut(s) 155, 624
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 1 cut(s) 56
BsiWI CGTACG 1 cut(s) 417
BsmAI GTCTC 3 cut(s) 421, 677, 888
BsmBI CGTCTC 2 cut(s) 421, 677
BsmI GAATGC 1 cut(s) 318
BsnI GGCC 2 cut(s) 155, 624
Bso31I GGTCTC 1 cut(s) 888
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 5 cut(s) 213, 268, 288, 634, 826
BspACI CCGC 7 cut(s) 24, 27, 59, 156, 366, 369, 382
BspANI GGCC 2 cut(s) 155, 624
BspCNI CTCAG 2 cut(s) 56, 786
BspFNI CGCG 5 cut(s) 95, 413, 494, 508, 567
BspLI GGNNCC 1 cut(s) 310
BspPI GGATC 1 cut(s) 263
BspQI GCTCTTC 1 cut(s) 72
BspTNI GGTCTC 1 cut(s) 888
BsrDI GCAATG 4 cut(s) 204, 254, 474, 897
BsrI ACTGG 1 cut(s) 180
BssMI GATC 5 cut(s) 213, 268, 288, 634, 826
BssNI GRCGYC 1 cut(s) 251
BssSI CACGAG 1 cut(s) 885
Bst2BI CACGAG 1 cut(s) 885
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 2 cut(s) 42, 72
BstACI GRCGYC 1 cut(s) 251
BstBAI YACGTR 2 cut(s) 421, 620
BstC8I GCNNGC 2 cut(s) 496, 569
BstDEI CTNAG 3 cut(s) 43, 530, 794
BstF5I GGATG 5 cut(s) 73, 208, 441, 676, 902
BstFNI CGCG 5 cut(s) 95, 413, 494, 508, 567
BstHHI GCGC 2 cut(s) 381, 508
BstKTI GATC 5 cut(s) 216, 271, 291, 637, 829
BstMAI GTCTC 3 cut(s) 421, 677, 888
BstMBI GATC 5 cut(s) 213, 268, 288, 634, 826
BstMWI GCNNNNNNNGC 2 cut(s) 315, 362
BstUI CGCG 5 cut(s) 95, 413, 494, 508, 567
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BsuI GTATCC 1 cut(s) 63
BsuRI GGCC 2 cut(s) 155, 624
BtrI CACGTC 1 cut(s) 349
BtsCI GGATG 5 cut(s) 73, 208, 441, 676, 902
BtsIMutI CAGTG 1 cut(s) 791
Cac8I GCNNGC 2 cut(s) 496, 569
CfoI GCGC 2 cut(s) 381, 508
CseI GACGC 3 cut(s) 240, 362, 402
Csp6I GTAC 5 cut(s) 409, 418, 472, 527, 674
CspCI CAANNNNNGTGG 2 cut(s) 684, 719
CviAII CATG 1 cut(s) 301
CviQI GTAC 5 cut(s) 409, 418, 472, 527, 674
DdeI CTNAG 3 cut(s) 43, 530, 794
DpnI GATC 5 cut(s) 215, 270, 290, 636, 828
DpnII GATC 5 cut(s) 213, 268, 288, 634, 826
DrdI GACNNNNNNGTC 1 cut(s) 691
DseDI GACNNNNNNGTC 1 cut(s) 691
EaeI YGGCCR 2 cut(s) 153, 622
Eam1104I CTCTTC 2 cut(s) 42, 72
EarI CTCTTC 2 cut(s) 42, 72
Eco31I GGTCTC 1 cut(s) 888
Esp3I CGTCTC 2 cut(s) 421, 677
FaeI CATG 1 cut(s) 304
FaiI YATR 8 cut(s) 302, 503, 546, 654, 722, 740, 768, 849
FatI CATG 1 cut(s) 300
FblI GTMKAC 1 cut(s) 616
Fnu4HI GCNGC 6 cut(s) 24, 27, 156, 366, 369, 382
FokI GGATG 5 cut(s) 60, 215, 448, 683, 889
Fsp4HI GCNGC 6 cut(s) 24, 27, 156, 366, 369, 382
GlaI GCGC 2 cut(s) 380, 507
GluI GCNGC 6 cut(s) 24, 27, 156, 366, 369, 382
GsuI CTGGAG 1 cut(s) 197
HaeIII GGCC 2 cut(s) 155, 624
HapII CCGG 1 cut(s) 56
HgaI GACGC 3 cut(s) 240, 362, 402
HhaI GCGC 2 cut(s) 381, 508
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 1 cut(s) 304
Hin6I GCGC 2 cut(s) 379, 506
HinP1I GCGC 2 cut(s) 379, 506
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 4 cut(s) 111, 692, 798, 919
HpaII CCGG 1 cut(s) 56
HphI GGTGA 2 cut(s) 391, 436
Hpy166II GTNNAC 2 cut(s) 352, 617
Hpy188I TCNGA 6 cut(s) 46, 108, 268, 324, 335, 648
Hpy188III TCNNGA 5 cut(s) 170, 208, 217, 272, 283
Hpy8I GTNNAC 2 cut(s) 352, 617
Hpy99I CGWCG 4 cut(s) 95, 130, 418, 611
HpyAV CCTTC 3 cut(s) 682, 725, 802
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 5 cut(s) 79, 348, 420, 619, 663
HpyCH4V TGCA 6 cut(s) 84, 190, 197, 786, 847, 902
HpyF10VI GCNNNNNNNGC 2 cut(s) 315, 362
HpyF3I CTNAG 3 cut(s) 43, 530, 794
HpySE526I ACGT 5 cut(s) 79, 348, 420, 619, 663
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 1 cut(s) 304
HspAI GCGC 2 cut(s) 379, 506
Kzo9I GATC 5 cut(s) 213, 268, 288, 634, 826
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 3 cut(s) 18, 314, 715
LpnPI CCDG 4 cut(s) 23, 69, 161, 221
LweI GCATC 4 cut(s) 93, 171, 529, 911
MaeII ACGT 5 cut(s) 79, 348, 420, 619, 663
MaeIII GTNAC 1 cut(s) 799
MalI GATC 5 cut(s) 215, 270, 290, 636, 828
MboI GATC 5 cut(s) 213, 268, 288, 634, 826
MboII GAAGA 4 cut(s) 59, 59, 466, 469
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 480
MlsI TGGCCA 1 cut(s) 624
MluCI AATT 3 cut(s) 139, 192, 772
MluI ACGCGT 2 cut(s) 411, 565
MluNI TGGCCA 1 cut(s) 624
MlyI GAGTC 2 cut(s) 701, 807
MnlI CCTC 8 cut(s) 81, 111, 114, 177, 398, 706, 741, 750
Mox20I TGGCCA 1 cut(s) 624
MscI TGGCCA 1 cut(s) 624
MseI TTAA 4 cut(s) 134, 161, 573, 911
Msp20I TGGCCA 1 cut(s) 624
MspI CCGG 1 cut(s) 56
Mva1269I GAATGC 1 cut(s) 318
MvnI CGCG 5 cut(s) 95, 413, 494, 508, 567
MwoI GCNNNNNNNGC 2 cut(s) 315, 362
NdeII GATC 5 cut(s) 213, 268, 288, 634, 826
NlaIII CATG 1 cut(s) 304
NlaIV GGNNCC 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 799
PciSI GCTCTTC 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PctI GAATGC 1 cut(s) 318
PfeI GAWTC 2 cut(s) 111, 919
Pfl23II CGTACG 1 cut(s) 417
PkrI GCNGC 6 cut(s) 25, 28, 157, 367, 370, 383
PleI GAGTC 2 cut(s) 700, 806
PpsI GAGTC 2 cut(s) 700, 806
Ppu21I YACGTR 2 cut(s) 421, 620
PspLI CGTACG 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 310
PsuI RGATCY 1 cut(s) 268
RsaI GTAC 5 cut(s) 410, 419, 473, 528, 675
RsaNI GTAC 5 cut(s) 409, 418, 472, 527, 674
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 4 cut(s) 134, 161, 573, 911
SatI GCNGC 6 cut(s) 24, 27, 156, 366, 369, 382
Sau3AI GATC 5 cut(s) 213, 268, 288, 634, 826
SchI GAGTC 2 cut(s) 701, 807
SduI GDGCHC 1 cut(s) 480
SfaNI GCATC 4 cut(s) 93, 171, 529, 911
SmlI CTYRAG 2 cut(s) 168, 854
SmoI CTYRAG 2 cut(s) 168, 854
Sse9I AATT 3 cut(s) 139, 192, 772
SsiI CCGC 7 cut(s) 24, 27, 59, 156, 366, 369, 382
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 5 cut(s) 82, 351, 423, 622, 666
TaqI TCGA 3 cut(s) 218, 447, 917
TasI AATT 3 cut(s) 139, 192, 772
TatI WGTACW 2 cut(s) 526, 673
TauI GCSGC 6 cut(s) 26, 29, 158, 368, 371, 384
TfiI GAWTC 2 cut(s) 111, 919
Tru1I TTAA 4 cut(s) 134, 161, 573, 911
Tru9I TTAA 4 cut(s) 134, 161, 573, 911
TscAI CASTG 1 cut(s) 798
TseFI GTSAC 1 cut(s) 799
Tsp45I GTSAC 1 cut(s) 799
TspDTI ATGAA 1 cut(s) 317
TspGWI ACGGA 3 cut(s) 5, 79, 114
TspRI CASTG 1 cut(s) 798
XapI RAATTY 1 cut(s) 772
XmiI GTMKAC 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.