Rh5AG007400

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0009111
Physical Location & Seq
Forward (+)
508660 .. 510430
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG007400.1

Sequence Viewer

Length: 1362 bp
ATGGACGACTCTAAAGACACCAACTCTTCTTCTCCAGTTTCGTCTTCTGATGTCCCTCCAACCAAACCCCAACAACAATCAGAGGCTTATTGTTATCCCAAACATGAAATCGGGTTCGAAAAGGCTAACTCTGTTCCAATTCCATTATATTCACAAGATATTTGGAGAGGTCAAAGTGACAACGCTGCAGGACAACTTTTGGGTTCAAACAACCTGAATGGGCAAGTTTTGACAAGGAATGGGAACCCTAGACTAGATGCATATGCTGGTTTTCAATCTCATGAAGTGGCTGAGAAGAGTGGAATGTATTCAAATAATAATTGGTATCGAAGTTCAGGAATATTGAATGACATGGGGAACCAATCAAGCTTTGGCAATGACTCGATGCTGGGAAGTAGCTATATAGCTCAAACGGCTATGACTCAATATGGCTCACAGTACTTGCTAGATAGCTTGTTGACTAGGGACCCCGTAGTTACAAATTTTATATTTGAAGGTGTTTTCGATTTCCTATTCAAGGTGATGAATGATTCAAATGGACATCATCTTTTTGGGAAGCTAGTTCAGTCATGCAGCGATAACCAGTTAGGCCTCATTGTAGCAAAGATAACTTTGAATGTCCAATGGCTTATCAACATATCAGTCGGCAGATATGGCTCAAAATCGGTTCAGAGGCTGATCAAGGTGCTTGAGAAATCACCCTTGATTGACATTGTCATAGCAGCCCTATCCTGTGGATTTGAAGAACTGATGACCAATCGCTCAGGATCCTTTGTTATATTGAAGTGCTTAAACCTCCTCGATACTGAGAAAAATCAGAAACTATATGAAGCTGCTGTAAATCTTTGCATCCCATTGGCTCAAAATGAAAAGGGATGTATATACTTGAATGAGTTCATCACGAACAGTAAAGCTCCATATAGAGAGACACTCATGGGTGTGATCTCAAGCAAATCAAAATTCCTTTCTCAGGATCCTTCAGGGAACTTTGTTGTTCAGCACATCCTCGGACTTCACAATCCCATTTACGGTGAGCGCATATGTTTGGAGCTGAGAGAACTTTATATACAGCTCTCGCTACAAAAAGGTGGCAGTCACGTTGTGGAGAAGTGCTTAAACTCATCCGGGATGGTTCATGTGGTAAGTGTTCTTCTCAAGTATGAAAAACTTTGGCAAGTTGCGCGGGATCAATATGGAAACTACGTGATCCAAACAGCATTGAAGACCACAAAACGGGCGAATAGTCCCCTTCATCAAATGCTTATATCGAAGCTCGTACAGAATCGGAATGAACTTGTGATTGGATTTGGAAGGAAAGTGCTTGGTTTGATTGACAATGGGATCCCACTAGACCAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

453

Amino Acids

50.31

Weight (kDa)

7.57

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01410 AT1G21620 AT1G35850 AT1G35850 AT5G59280 AT5G60110 AT5G60180 AT5G64490
fragaria_vesca FvH4_3g00680
malus_domestica MD05G1360200.v1.1
prunus_persica Prupe.4G007700_v2.0.a1
pyrus_communis pycom05g32520
rosa_chinensis RchiOBHm_Chr5g0000911
rosa_laevigata RLG00000030906
rosa_roxburghii Rroxscaffold_1G00075340
rosa_rugosa Rorug04G0387800 Rorug04G0387900.1
rosa_samantha Rh5AG007400 Rh5BG009500 Rh5CG008100 Rh5DG008100
rosa_wichuraiana Rw5G000770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1183
AciI CCGC 1 cut(s) 1183
AclWI GGATC 8 cut(s) 762, 775, 968, 981, 1194, 1201, 1336, 1349
AcsI RAATTY 2 cut(s) 481, 959
AcuI CTGAAG 1 cut(s) 963
AdeI CACNNNGTG 1 cut(s) 1102
AfaI GTAC 2 cut(s) 440, 1278
AfiI CCNNNNNNNGG 4 cut(s) 517, 970, 1028, 1233
AjuI GAANNNNNNNTTGG 2 cut(s) 1284, 1316
Alw26I GTCTC 1 cut(s) 920
AlwI GGATC 8 cut(s) 762, 775, 968, 981, 1194, 1201, 1336, 1349
AlwNI CAGNNNCTG 1 cut(s) 676
AoxI GGCC 1 cut(s) 589
ApeKI GCWGC 4 cut(s) 185, 573, 722, 833
ApoI RAATTY 2 cut(s) 481, 959
Asp700I GAANNNNTTC 2 cut(s) 307, 893
AspLEI GCGC 2 cut(s) 1038, 1183
AspS9I GGNCC 1 cut(s) 466
AsuC2I CCSGG 1 cut(s) 1126
AsuHPI GGTGA 3 cut(s) 532, 690, 1043
AsuII TTCGAA 1 cut(s) 117
AvaII GGWCC 1 cut(s) 466
BamHI GGATCC 3 cut(s) 767, 973, 1341
BarI GAAGNNNNNNTAC 2 cut(s) 1134, 1166
BbsI GAAGAC 2 cut(s) 36, 1229
BbvI GCAGC 4 cut(s) 172, 585, 734, 820
BccI CCATC 1 cut(s) 1123
BceAI ACGGC 1 cut(s) 429
BclI TGATCA 1 cut(s) 678
BcnI CCSGG 1 cut(s) 1126
BcoDI GTCTC 1 cut(s) 920
BfaI CTAG 6 cut(s) 249, 254, 446, 462, 560, 1349
BfmI CTRYAG 1 cut(s) 186
BisI GCNGC 4 cut(s) 186, 574, 723, 834
BlsI GCNGC 4 cut(s) 187, 575, 724, 835
BmcAI AGTACT 1 cut(s) 440
Bme1390I CCNGG 1 cut(s) 1126
Bme18I GGWCC 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 7 cut(s) 245, 359, 467, 468, 769, 975, 1343
BmrFI CCNGG 1 cut(s) 1126
BmsI GCATC 3 cut(s) 247, 375, 858
BpiI GAAGAC 2 cut(s) 36, 1229
BplI GAGNNNNNCTC 2 cut(s) 915, 947
BpmI CTGGAG 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 763
Bpu14I TTCGAA 1 cut(s) 117
BpuEI CTTGAG 3 cut(s) 710, 931, 1139
BpuMI CCSGG 1 cut(s) 1126
BsaAI YACGTR 1 cut(s) 1204
BsaJI CCNNGG 1 cut(s) 1006
Bsc4I CCNNNNNNNGG 4 cut(s) 517, 970, 1028, 1233
Bse1I ACTGG 2 cut(s) 35, 583
Bse3DI GCAATG 1 cut(s) 382
BseDI CCNNGG 1 cut(s) 1006
BseGI GGATG 5 cut(s) 849, 881, 1002, 1121, 1134
BseLI CCNNNNNNNGG 4 cut(s) 517, 970, 1028, 1233
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 5 cut(s) 282, 777, 798, 983, 1043
BseNI ACTGG 2 cut(s) 35, 583
BseRI GAGGAG 1 cut(s) 788
BseXI GCAGC 4 cut(s) 172, 585, 734, 820
BseYI CCCAGC 1 cut(s) 388
Bsh1236I CGCG 1 cut(s) 1183
BshFI GGCC 1 cut(s) 591
BsiSI CCGG 1 cut(s) 1125
BslFI GGGAC 3 cut(s) 38, 479, 1230
BslI CCNNNNNNNGG 4 cut(s) 517, 970, 1028, 1233
BsmAI GTCTC 1 cut(s) 920
BsmFI GGGAC 3 cut(s) 38, 479, 1230
BsnI GGCC 1 cut(s) 591
Bsp119I TTCGAA 1 cut(s) 117
Bsp143I GATC 7 cut(s) 678, 767, 942, 973, 1186, 1206, 1341
BspACI CCGC 1 cut(s) 1183
BspANI GGCC 1 cut(s) 591
BspCNI CTCAG 5 cut(s) 283, 776, 799, 982, 1044
BspFNI CGCG 1 cut(s) 1183
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 7 cut(s) 245, 359, 467, 468, 769, 975, 1343
BspMAI CTGCAG 1 cut(s) 190
BspPI GGATC 8 cut(s) 762, 775, 968, 981, 1194, 1201, 1336, 1349
BspT104I TTCGAA 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 382
BsrI ACTGG 2 cut(s) 35, 583
BssECI CCNNGG 1 cut(s) 1006
BssMI GATC 7 cut(s) 678, 767, 942, 973, 1186, 1206, 1341
Bst4CI ACNGT 3 cut(s) 438, 908, 1031
Bst6I CTCTTC 2 cut(s) 31, 290
BstBAI YACGTR 1 cut(s) 1204
BstBI TTCGAA 1 cut(s) 117
BstDEI CTNAG 5 cut(s) 291, 763, 807, 969, 1052
BstENI CCTNNNNNAGG 2 cut(s) 515, 968
BstF5I GGATG 5 cut(s) 849, 881, 1002, 1121, 1134
BstFNI CGCG 1 cut(s) 1183
BstHHI GCGC 2 cut(s) 1038, 1183
BstKTI GATC 7 cut(s) 681, 770, 945, 976, 1189, 1209, 1344
BstMAI GTCTC 1 cut(s) 920
BstMBI GATC 7 cut(s) 678, 767, 942, 973, 1186, 1206, 1341
BstMWI GCNNNNNNNGC 3 cut(s) 413, 654, 1180
BstSCI CCNGG 1 cut(s) 1124
BstSFI CTRYAG 1 cut(s) 186
BstUI CGCG 1 cut(s) 1183
BstV1I GCAGC 4 cut(s) 172, 585, 734, 820
BstV2I GAAGAC 2 cut(s) 36, 1229
BstX2I RGATCY 3 cut(s) 767, 973, 1341
BstYI RGATCY 3 cut(s) 767, 973, 1341
BsuRI GGCC 1 cut(s) 591
BtsCI GGATG 5 cut(s) 849, 881, 1002, 1121, 1134
CaiI CAGNNNCTG 1 cut(s) 676
CciI TCATGA 1 cut(s) 280
CfoI GCGC 2 cut(s) 1038, 1183
Cfr13I GGNCC 1 cut(s) 466
Csp6I GTAC 2 cut(s) 439, 1277
CviAII CATG 6 cut(s) 104, 281, 352, 570, 934, 1136
CviQI GTAC 2 cut(s) 439, 1277
DdeI CTNAG 5 cut(s) 291, 763, 807, 969, 1052
DpnI GATC 7 cut(s) 680, 769, 944, 975, 1188, 1208, 1343
DpnII GATC 7 cut(s) 678, 767, 942, 973, 1186, 1206, 1341
DraIII CACNNNGTG 1 cut(s) 1102
Eam1104I CTCTTC 2 cut(s) 31, 290
EarI CTCTTC 2 cut(s) 31, 290
Eco147I AGGCCT 1 cut(s) 591
Eco47I GGWCC 1 cut(s) 466
Eco57I CTGAAG 1 cut(s) 963
EcoNI CCTNNNNNAGG 2 cut(s) 515, 968
EcoO109I RGGNCCY 1 cut(s) 466
EcoT22I ATGCAT 1 cut(s) 262
FaeI CATG 6 cut(s) 107, 284, 355, 573, 937, 1139
FaqI GGGAC 3 cut(s) 38, 479, 1230
FatI CATG 6 cut(s) 103, 280, 351, 569, 933, 1135
FauI CCCGC 1 cut(s) 1176
FauNDI CATATG 2 cut(s) 262, 1040
FbaI TGATCA 1 cut(s) 678
Fnu4HI GCNGC 4 cut(s) 186, 574, 723, 834
FokI GGATG 5 cut(s) 836, 888, 989, 1108, 1141
Fsp4HI GCNGC 4 cut(s) 186, 574, 723, 834
FspBI CTAG 6 cut(s) 249, 254, 446, 462, 560, 1349
GlaI GCGC 2 cut(s) 1037, 1182
GluI GCNGC 4 cut(s) 186, 574, 723, 834
GsaI CCCAGC 1 cut(s) 392
GsuI CTGGAG 1 cut(s) 18
HaeIII GGCC 1 cut(s) 591
HapII CCGG 1 cut(s) 1125
HhaI GCGC 2 cut(s) 1038, 1183
Hin1II CATG 6 cut(s) 107, 284, 355, 573, 937, 1139
Hin6I GCGC 2 cut(s) 1036, 1181
HinP1I GCGC 2 cut(s) 1036, 1181
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HindIII AAGCTT 1 cut(s) 367
HinfI GANTC 5 cut(s) 8, 380, 421, 530, 1282
HpaII CCGG 1 cut(s) 1125
HphI GGTGA 3 cut(s) 532, 690, 1043
Hpy166II GTNNAC 1 cut(s) 459
Hpy188I TCNGA 6 cut(s) 49, 82, 672, 819, 1010, 1287
Hpy188III TCNNGA 5 cut(s) 281, 336, 765, 901, 971
Hpy8I GTNNAC 1 cut(s) 459
HpyAV CCTTC 4 cut(s) 488, 987, 1259, 1305
HpyCH4III ACNGT 3 cut(s) 438, 908, 1031
HpyCH4IV ACGT 2 cut(s) 1098, 1203
HpyCH4V TGCA 4 cut(s) 188, 260, 573, 849
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 654, 1180
HpyF3I CTNAG 5 cut(s) 291, 763, 807, 969, 1052
HpySE526I ACGT 2 cut(s) 1098, 1203
Hsp92II CATG 6 cut(s) 107, 284, 355, 573, 937, 1139
HspAI GCGC 2 cut(s) 1036, 1181
KflI GGGWCCC 1 cut(s) 466
Ksp22I TGATCA 1 cut(s) 678
Kzo9I GATC 7 cut(s) 678, 767, 942, 973, 1186, 1206, 1341
LmnI GCTCC 2 cut(s) 919, 1048
Lsp1109I GCAGC 4 cut(s) 172, 585, 734, 820
LweI GCATC 3 cut(s) 247, 375, 858
MaeI CTAG 6 cut(s) 249, 254, 446, 462, 560, 1349
MaeII ACGT 2 cut(s) 1098, 1203
MaeIII GTNAC 3 cut(s) 176, 475, 1094
MalI GATC 7 cut(s) 680, 769, 944, 975, 1188, 1208, 1343
MboI GATC 7 cut(s) 678, 767, 942, 973, 1186, 1206, 1341
MboII GAAGA 7 cut(s) 18, 21, 36, 307, 755, 1142, 1234
MflI RGATCY 3 cut(s) 767, 973, 1341
MluCI AATT 4 cut(s) 138, 319, 481, 959
MlyI GAGTC 3 cut(s) 2, 374, 415
MmeI TCCRAC 1 cut(s) 83
MnlI CCTC 8 cut(s) 66, 76, 161, 602, 666, 806, 809, 1016
Mph1103I ATGCAT 1 cut(s) 262
MroXI GAANNNNTTC 2 cut(s) 307, 893
MseI TTAA 2 cut(s) 791, 1115
MslI CAYNNNNRTG 1 cut(s) 938
MspI CCGG 1 cut(s) 1125
MspR9I CCNGG 1 cut(s) 1126
MvnI CGCG 1 cut(s) 1183
MwoI GCNNNNNNNGC 3 cut(s) 413, 654, 1180
NciI CCSGG 1 cut(s) 1126
NdeI CATATG 2 cut(s) 262, 1040
NdeII GATC 7 cut(s) 678, 767, 942, 973, 1186, 1206, 1341
NlaIII CATG 6 cut(s) 107, 284, 355, 573, 937, 1139
NlaIV GGNNCC 7 cut(s) 245, 359, 467, 468, 769, 975, 1343
NmuCI GTSAC 2 cut(s) 176, 1094
NsiI ATGCAT 1 cut(s) 262
NspV TTCGAA 1 cut(s) 117
PagI TCATGA 1 cut(s) 280
PceI AGGCCT 1 cut(s) 591
PdmI GAANNNNTTC 2 cut(s) 307, 893
PfeI GAWTC 2 cut(s) 530, 1282
PflFI GACNNNGTC 1 cut(s) 713
PfoI TCCNGGA 1 cut(s) 1124
PkrI GCNGC 4 cut(s) 187, 575, 724, 835
PleI GAGTC 3 cut(s) 2, 374, 415
PpsI GAGTC 3 cut(s) 2, 374, 415
Ppu21I YACGTR 1 cut(s) 1204
PpuMI RGGWCCY 1 cut(s) 466
Psp5II RGGWCCY 1 cut(s) 466
PspFI CCCAGC 1 cut(s) 388
PspN4I GGNNCC 7 cut(s) 245, 359, 467, 468, 769, 975, 1343
PspPI GGNCC 1 cut(s) 466
PspPPI RGGWCCY 1 cut(s) 466
PsrI GAACNNNNNNTAC 2 cut(s) 1050, 1082
PstI CTGCAG 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 676
PsuI RGATCY 3 cut(s) 767, 973, 1341
PsyI GACNNNGTC 1 cut(s) 713
RsaI GTAC 2 cut(s) 440, 1278
RsaNI GTAC 2 cut(s) 439, 1277
RseI CAYNNNNRTG 1 cut(s) 938
SaqAI TTAA 2 cut(s) 791, 1115
SatI GCNGC 4 cut(s) 186, 574, 723, 834
Sau3AI GATC 7 cut(s) 678, 767, 942, 973, 1186, 1206, 1341
Sau96I GGNCC 1 cut(s) 466
ScaI AGTACT 1 cut(s) 440
SchI GAGTC 3 cut(s) 2, 374, 415
ScrFI CCNGG 1 cut(s) 1126
SfaNI GCATC 3 cut(s) 247, 375, 858
SfcI CTRYAG 1 cut(s) 186
SfuI TTCGAA 1 cut(s) 117
SinI GGWCC 1 cut(s) 466
SmiMI CAYNNNNRTG 1 cut(s) 938
SmlI CTYRAG 3 cut(s) 689, 946, 1154
SmoI CTYRAG 3 cut(s) 689, 946, 1154
Sse9I AATT 4 cut(s) 138, 319, 481, 959
SseBI AGGCCT 1 cut(s) 591
SsiI CCGC 1 cut(s) 1183
SspI AATATT 1 cut(s) 342
SspMI CTAG 6 cut(s) 249, 254, 446, 462, 560, 1349
StuI AGGCCT 1 cut(s) 591
StyD4I CCNGG 1 cut(s) 1124
TaaI ACNGT 3 cut(s) 438, 908, 1031
TaiI ACGT 2 cut(s) 1101, 1206
TaqI TCGA 6 cut(s) 117, 328, 383, 504, 801, 1268
TasI AATT 4 cut(s) 138, 319, 481, 959
TatI WGTACW 1 cut(s) 438
TfiI GAWTC 2 cut(s) 530, 1282
Tru1I TTAA 2 cut(s) 791, 1115
Tru9I TTAA 2 cut(s) 791, 1115
TseFI GTSAC 2 cut(s) 176, 1094
TseI GCWGC 4 cut(s) 185, 573, 722, 833
Tsp45I GTSAC 2 cut(s) 176, 1094
Tth111I GACNNNGTC 1 cut(s) 713
VpaK11BI GGWCC 1 cut(s) 466
XagI CCTNNNNNAGG 2 cut(s) 515, 968
XapI RAATTY 2 cut(s) 481, 959
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XmnI GAANNNNTTC 2 cut(s) 307, 893
XspI CTAG 6 cut(s) 249, 254, 446, 462, 560, 1349
ZrmI AGTACT 1 cut(s) 440
Zsp2I ATGCAT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.