RLG00000030906

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009111
Physical Location & Seq
Forward (+)
544746 .. 546644
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030906

Sequence Viewer

Length: 1491 bp
ATGTCGATTTCGATGTCTGTTTCAGGTGGCACTAGTCCTTGTGGACCTCTCAACCCCAAAAAAACTTGGGTGGATTCCTCGTTTCAAGAAGAAGACCTTTGCCTGTATTTCGGTAAGTTTCGGGTAAACATGGACAACTCTAAAGACACCAACTCTTCTTCTCCAGGTTCGTCTTCTAGTGTCCCTCCAACCAAACCCCAACAACAATCAGAGGCTTATTGTTATCCCAAACATGAAATCGGGTTCGAAAAGTCTAACTCTGTTCCAATTCCATTATATTCACAAGATATTTGGAGAGGTCAAAGTGACAACGCTGCAGGACAACGGTTGGGTTCAAACAACCTGAATGGGCAAGTTTTGACAAGGAATGGGAACCCTAGACTAGATGCATATGCTGGTTTTCAATCTCATGAAGTGGCTGAGAAGAGTGGAATGTATTCAAATAATAATTGGTATCGAAGTTCAGGAATATTGAATGGCATGGGGAACCAATCAAGCTTTGGCAATGCCTCGATGCTGGGAAGTAGCTATATAGCTCAAACGGCTATGACTCAATATGGCTCACAGTACTTGCTAGATAGCTTGTTGACTAAGGACCCCGTAGTTACAAATTTTATATTTGAAGGTGTTTTCAATTTCCTATTCGAGGTGATGAATGATTCAAATGGACATCATCTTTTTGGGAAGCTAGTTCAGTCATGCAGCGATAACCAGTTAGGCCTCATTATAGCAAAGATAACTTTGAATGTCCAATTGCTTATCAACATATCAGTCGGCAGATATGGCTCAAAATCGGTTCAGAGGCTGATCAAGGTGCTTGAGAAATCACCCTTGATTGACATTGTCATAGCAGCCCTATCCTGTGGATTTGAAGAACTGATGACCAATCGCTCAGGATCCTTTGTTATATTGAAGTGCTTAAACCTCCTCGACACTGAGAAAAATCAGAAACTATATGAAGCTGCTGTAAATCTTTGCATCCCATTGGCTCAGAATGAAAAGGGATGTATATACTTGAATGAGTTCATCACCAACAGTAAAGCTCCATATAGAGAGACACTCATGGGTGAGATCTCAAGCAAATCAAAATTCCTTTCTCAGGATCCTTCAGGGAACTTTGTTGTTCAGCACATCCTCGGACTCCACAATCCCATTTACGGTGAGCGCATATGTTTGGAGCTGAGAGAACTTTATATACAGCTCTCGCAACAAAAAGGTGGCAGTCACGTTGTGGAGAAGTGCTTAAACTCATCCGGGATGGTTCATGTGGTAAGCGTTCTTCTCAATTATGAAAAACTTTGGCAAGTGGCGAGGGATCAATATGGAAACTACGTGATCCAAACAGCATTGAAGACCACAAAACGCGCGAGTAGTCCCCTTCATCAAATGCTTATATCGAAGCTCGTACAGAATCGGAATGAACTTGTGATTGGATTTGGAAGGAAAGTGCTTGGTTTGATTGACAATGGGATCCCACTAGACCGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

497

Amino Acids

54.87

Weight (kDa)

8.39

Isoelectric Point (pI)

32.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01410 AT1G21620 AT1G35850 AT1G35850 AT5G59280 AT5G60110 AT5G60180 AT5G64490
fragaria_vesca FvH4_3g00680
malus_domestica MD05G1360200.v1.1
prunus_persica Prupe.4G007700_v2.0.a1
pyrus_communis pycom05g32520
rosa_chinensis RchiOBHm_Chr5g0000911
rosa_laevigata RLG00000030906
rosa_roxburghii Rroxscaffold_1G00075340
rosa_rugosa Rorug04G0387800 Rorug04G0387900.1
rosa_samantha Rh5AG007400 Rh5BG009500 Rh5CG008100 Rh5DG008100
rosa_wichuraiana Rw5G000770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 1365, 1367
AclWI GGATC 8 cut(s) 891, 904, 1097, 1110, 1323, 1330, 1465, 1478
AcsI RAATTY 2 cut(s) 610, 1088
AcuI CTGAAG 1 cut(s) 1092
AdeI CACNNNGTG 1 cut(s) 1231
AfaI GTAC 2 cut(s) 569, 1407
AfiI CCNNNNNNNGG 3 cut(s) 646, 1099, 1157
AhlI ACTAGT 1 cut(s) 32
AjnI CCWGG 1 cut(s) 163
AjuI GAANNNNNNNTTGG 2 cut(s) 1413, 1445
Alw26I GTCTC 1 cut(s) 1049
AlwI GGATC 8 cut(s) 891, 904, 1097, 1110, 1323, 1330, 1465, 1478
AlwNI CAGNNNCTG 1 cut(s) 805
AoxI GGCC 1 cut(s) 718
ApeKI GCWGC 4 cut(s) 314, 702, 851, 962
ApoI RAATTY 2 cut(s) 610, 1088
Asp700I GAANNNNTTC 2 cut(s) 436, 1022
AspLEI GCGC 2 cut(s) 1167, 1367
AspS9I GGNCC 2 cut(s) 44, 595
AsuC2I CCSGG 1 cut(s) 1255
AsuHPI GGTGA 5 cut(s) 661, 819, 1021, 1079, 1172
AsuII TTCGAA 1 cut(s) 246
AvaII GGWCC 2 cut(s) 44, 595
BamHI GGATCC 3 cut(s) 896, 1102, 1470
BarI GAAGNNNNNNTAC 2 cut(s) 1263, 1295
BbsI GAAGAC 3 cut(s) 99, 165, 1358
BbvI GCAGC 4 cut(s) 301, 714, 863, 949
BccI CCATC 1 cut(s) 1252
BceAI ACGGC 1 cut(s) 558
BciT130I CCWGG 1 cut(s) 165
BclI TGATCA 1 cut(s) 807
BcnI CCSGG 1 cut(s) 1255
BcoDI GTCTC 1 cut(s) 1049
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 7 cut(s) 33, 177, 378, 383, 575, 689, 1478
BfmI CTRYAG 1 cut(s) 315
BglII AGATCT 1 cut(s) 1071
BisI GCNGC 4 cut(s) 315, 703, 852, 963
BlsI GCNGC 4 cut(s) 316, 704, 853, 964
BmcAI AGTACT 1 cut(s) 569
Bme1390I CCNGG 2 cut(s) 165, 1255
Bme18I GGWCC 2 cut(s) 44, 595
BmgT120I GGNCC 2 cut(s) 44, 595
BmiI GGNNCC 6 cut(s) 374, 488, 597, 898, 1104, 1472
BmrFI CCNGG 2 cut(s) 165, 1255
BmsI GCATC 3 cut(s) 376, 504, 987
BpiI GAAGAC 3 cut(s) 99, 165, 1358
BplI GAGNNNNNCTC 2 cut(s) 1044, 1076
BpmI CTGGAG 1 cut(s) 147
Bpu10I CCTNAGC 1 cut(s) 892
Bpu14I TTCGAA 1 cut(s) 246
BpuEI CTTGAG 2 cut(s) 839, 1060
BpuMI CCSGG 1 cut(s) 1255
BsaAI YACGTR 1 cut(s) 1333
BsaJI CCNNGG 1 cut(s) 1135
Bsc4I CCNNNNNNNGG 3 cut(s) 646, 1099, 1157
Bse1I ACTGG 1 cut(s) 712
Bse3DI GCAATG 1 cut(s) 511
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 1135
BseGI GGATG 5 cut(s) 978, 1010, 1131, 1250, 1263
BseLI CCNNNNNNNGG 3 cut(s) 646, 1099, 1157
BseMI GCAATG 1 cut(s) 511
BseMII CTCAG 6 cut(s) 411, 906, 927, 1004, 1112, 1172
BseNI ACTGG 1 cut(s) 712
BseRI GAGGAG 1 cut(s) 917
BseXI GCAGC 4 cut(s) 301, 714, 863, 949
BseYI CCCAGC 1 cut(s) 517
Bsh1236I CGCG 2 cut(s) 1365, 1367
BshFI GGCC 1 cut(s) 720
BsiSI CCGG 1 cut(s) 1254
BslFI GGGAC 2 cut(s) 167, 1359
BslI CCNNNNNNNGG 3 cut(s) 646, 1099, 1157
BsmAI GTCTC 1 cut(s) 1049
BsmFI GGGAC 2 cut(s) 167, 1359
BsnI GGCC 1 cut(s) 720
Bsp119I TTCGAA 1 cut(s) 246
Bsp143I GATC 7 cut(s) 807, 896, 1071, 1102, 1315, 1335, 1470
BspANI GGCC 1 cut(s) 720
BspCNI CTCAG 6 cut(s) 412, 905, 928, 1003, 1111, 1173
BspFNI CGCG 2 cut(s) 1365, 1367
BspHI TCATGA 1 cut(s) 409
BspLI GGNNCC 6 cut(s) 374, 488, 597, 898, 1104, 1472
BspMAI CTGCAG 1 cut(s) 319
BspPI GGATC 8 cut(s) 891, 904, 1097, 1110, 1323, 1330, 1465, 1478
BspT104I TTCGAA 1 cut(s) 246
BsrDI GCAATG 1 cut(s) 511
BsrI ACTGG 1 cut(s) 712
BssECI CCNNGG 1 cut(s) 1135
BssMI GATC 7 cut(s) 807, 896, 1071, 1102, 1315, 1335, 1470
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 4 cut(s) 327, 567, 1037, 1160
Bst6I CTCTTC 2 cut(s) 160, 419
BstBAI YACGTR 1 cut(s) 1333
BstBI TTCGAA 1 cut(s) 246
BstDEI CTNAG 7 cut(s) 420, 591, 892, 936, 990, 1098, 1181
BstENI CCTNNNNNAGG 2 cut(s) 644, 1097
BstF5I GGATG 5 cut(s) 978, 1010, 1131, 1250, 1263
BstFNI CGCG 2 cut(s) 1365, 1367
BstHHI GCGC 2 cut(s) 1167, 1367
BstKTI GATC 7 cut(s) 810, 899, 1074, 1105, 1318, 1338, 1473
BstMAI GTCTC 1 cut(s) 1049
BstMBI GATC 7 cut(s) 807, 896, 1071, 1102, 1315, 1335, 1470
BstMWI GCNNNNNNNGC 2 cut(s) 542, 783
BstNI CCWGG 1 cut(s) 165
BstSCI CCNGG 2 cut(s) 163, 1253
BstSFI CTRYAG 1 cut(s) 315
BstUI CGCG 2 cut(s) 1365, 1367
BstV1I GCAGC 4 cut(s) 301, 714, 863, 949
BstV2I GAAGAC 3 cut(s) 99, 165, 1358
BstX2I RGATCY 4 cut(s) 896, 1071, 1102, 1470
BstYI RGATCY 4 cut(s) 896, 1071, 1102, 1470
BsuRI GGCC 1 cut(s) 720
BtsCI GGATG 5 cut(s) 978, 1010, 1131, 1250, 1263
BtsIMutI CAGTG 1 cut(s) 933
CaiI CAGNNNCTG 1 cut(s) 805
CciI TCATGA 1 cut(s) 409
CfoI GCGC 2 cut(s) 1167, 1367
Cfr13I GGNCC 2 cut(s) 44, 595
Csp6I GTAC 2 cut(s) 568, 1406
CviAII CATG 7 cut(s) 130, 233, 410, 481, 699, 1063, 1265
CviQI GTAC 2 cut(s) 568, 1406
DdeI CTNAG 7 cut(s) 420, 591, 892, 936, 990, 1098, 1181
DpnI GATC 7 cut(s) 809, 898, 1073, 1104, 1317, 1337, 1472
DpnII GATC 7 cut(s) 807, 896, 1071, 1102, 1315, 1335, 1470
DraIII CACNNNGTG 1 cut(s) 1231
Eam1104I CTCTTC 2 cut(s) 160, 419
EarI CTCTTC 2 cut(s) 160, 419
Eco147I AGGCCT 1 cut(s) 720
Eco47I GGWCC 2 cut(s) 44, 595
Eco57I CTGAAG 1 cut(s) 1092
EcoNI CCTNNNNNAGG 2 cut(s) 644, 1097
EcoO109I RGGNCCY 1 cut(s) 595
EcoRII CCWGG 1 cut(s) 163
EcoT22I ATGCAT 1 cut(s) 391
FaeI CATG 7 cut(s) 133, 236, 413, 484, 702, 1066, 1268
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FaqI GGGAC 2 cut(s) 167, 1359
FatI CATG 7 cut(s) 129, 232, 409, 480, 698, 1062, 1264
FauNDI CATATG 2 cut(s) 391, 1169
FbaI TGATCA 1 cut(s) 807
Fnu4HI GCNGC 4 cut(s) 315, 703, 852, 963
FokI GGATG 5 cut(s) 965, 1017, 1118, 1237, 1270
Fsp4HI GCNGC 4 cut(s) 315, 703, 852, 963
FspBI CTAG 7 cut(s) 33, 177, 378, 383, 575, 689, 1478
GlaI GCGC 2 cut(s) 1166, 1366
GluI GCNGC 4 cut(s) 315, 703, 852, 963
GsaI CCCAGC 1 cut(s) 521
GsuI CTGGAG 1 cut(s) 147
HaeIII GGCC 1 cut(s) 720
HapII CCGG 1 cut(s) 1254
HhaI GCGC 2 cut(s) 1167, 1367
Hin1II CATG 7 cut(s) 133, 236, 413, 484, 702, 1066, 1268
Hin6I GCGC 2 cut(s) 1165, 1365
HinP1I GCGC 2 cut(s) 1165, 1365
HincII GTYRAC 1 cut(s) 588
HindII GTYRAC 1 cut(s) 588
HindIII AAGCTT 1 cut(s) 496
HinfI GANTC 5 cut(s) 74, 550, 659, 1140, 1411
HpaII CCGG 1 cut(s) 1254
HphI GGTGA 5 cut(s) 661, 819, 1021, 1079, 1172
Hpy166II GTNNAC 3 cut(s) 44, 127, 588
Hpy188I TCNGA 6 cut(s) 211, 801, 948, 993, 1139, 1416
Hpy188III TCNNGA 5 cut(s) 86, 410, 465, 894, 1100
Hpy8I GTNNAC 3 cut(s) 44, 127, 588
HpyAV CCTTC 4 cut(s) 617, 1116, 1388, 1434
HpyCH4III ACNGT 4 cut(s) 327, 567, 1037, 1160
HpyCH4IV ACGT 2 cut(s) 1227, 1332
HpyCH4V TGCA 4 cut(s) 317, 389, 702, 978
HpyF10VI GCNNNNNNNGC 2 cut(s) 542, 783
HpyF3I CTNAG 7 cut(s) 420, 591, 892, 936, 990, 1098, 1181
HpySE526I ACGT 2 cut(s) 1227, 1332
Hsp92II CATG 7 cut(s) 133, 236, 413, 484, 702, 1066, 1268
HspAI GCGC 2 cut(s) 1165, 1365
Ksp22I TGATCA 1 cut(s) 807
Kzo9I GATC 7 cut(s) 807, 896, 1071, 1102, 1315, 1335, 1470
LmnI GCTCC 2 cut(s) 1048, 1177
Lsp1109I GCAGC 4 cut(s) 301, 714, 863, 949
LweI GCATC 3 cut(s) 376, 504, 987
MaeI CTAG 7 cut(s) 33, 177, 378, 383, 575, 689, 1478
MaeII ACGT 2 cut(s) 1227, 1332
MaeIII GTNAC 3 cut(s) 305, 604, 1223
MalI GATC 7 cut(s) 809, 898, 1073, 1104, 1317, 1337, 1472
MboI GATC 7 cut(s) 807, 896, 1071, 1102, 1315, 1335, 1470
MboII GAAGA 9 cut(s) 101, 104, 147, 150, 165, 436, 884, 1271, 1363
MfeI CAATTG 1 cut(s) 752
MflI RGATCY 4 cut(s) 896, 1071, 1102, 1470
MluCI AATT 7 cut(s) 267, 448, 610, 634, 752, 1088, 1285
MlyI GAGTC 2 cut(s) 544, 1134
MmeI TCCRAC 1 cut(s) 212
Mph1103I ATGCAT 1 cut(s) 391
MroXI GAANNNNTTC 2 cut(s) 436, 1022
MseI TTAA 2 cut(s) 920, 1244
MspI CCGG 1 cut(s) 1254
MspR9I CCNGG 2 cut(s) 165, 1255
MunI CAATTG 1 cut(s) 752
MvaI CCWGG 1 cut(s) 165
MvnI CGCG 2 cut(s) 1365, 1367
MwoI GCNNNNNNNGC 2 cut(s) 542, 783
NciI CCSGG 1 cut(s) 1255
NdeI CATATG 2 cut(s) 391, 1169
NdeII GATC 7 cut(s) 807, 896, 1071, 1102, 1315, 1335, 1470
NlaIII CATG 7 cut(s) 133, 236, 413, 484, 702, 1066, 1268
NlaIV GGNNCC 6 cut(s) 374, 488, 597, 898, 1104, 1472
NmuCI GTSAC 2 cut(s) 305, 1223
NsiI ATGCAT 1 cut(s) 391
NspV TTCGAA 1 cut(s) 246
PagI TCATGA 1 cut(s) 409
PceI AGGCCT 1 cut(s) 720
PdmI GAANNNNTTC 2 cut(s) 436, 1022
PfeI GAWTC 3 cut(s) 74, 659, 1411
PflFI GACNNNGTC 1 cut(s) 842
PfoI TCCNGGA 1 cut(s) 1253
PkrI GCNGC 4 cut(s) 316, 704, 853, 964
PleI GAGTC 2 cut(s) 544, 1134
PpsI GAGTC 2 cut(s) 544, 1134
Ppu21I YACGTR 1 cut(s) 1333
PpuMI RGGWCCY 1 cut(s) 595
Psp5II RGGWCCY 1 cut(s) 595
Psp6I CCWGG 1 cut(s) 163
PspFI CCCAGC 1 cut(s) 517
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 6 cut(s) 374, 488, 597, 898, 1104, 1472
PspPI GGNCC 2 cut(s) 44, 595
PspPPI RGGWCCY 1 cut(s) 595
PsrI GAACNNNNNNTAC 2 cut(s) 1179, 1211
PstI CTGCAG 1 cut(s) 319
PstNI CAGNNNCTG 1 cut(s) 805
PsuI RGATCY 4 cut(s) 896, 1071, 1102, 1470
PsyI GACNNNGTC 1 cut(s) 842
RsaI GTAC 2 cut(s) 569, 1407
RsaNI GTAC 2 cut(s) 568, 1406
SaqAI TTAA 2 cut(s) 920, 1244
SatI GCNGC 4 cut(s) 315, 703, 852, 963
Sau3AI GATC 7 cut(s) 807, 896, 1071, 1102, 1315, 1335, 1470
Sau96I GGNCC 2 cut(s) 44, 595
ScaI AGTACT 1 cut(s) 569
SchI GAGTC 2 cut(s) 544, 1134
ScrFI CCNGG 2 cut(s) 165, 1255
SfaNI GCATC 3 cut(s) 376, 504, 987
SfcI CTRYAG 1 cut(s) 315
SfuI TTCGAA 1 cut(s) 246
SinI GGWCC 2 cut(s) 44, 595
SmlI CTYRAG 2 cut(s) 818, 1075
SmoI CTYRAG 2 cut(s) 818, 1075
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 7 cut(s) 267, 448, 610, 634, 752, 1088, 1285
SseBI AGGCCT 1 cut(s) 720
SspI AATATT 1 cut(s) 471
SspMI CTAG 7 cut(s) 33, 177, 378, 383, 575, 689, 1478
StuI AGGCCT 1 cut(s) 720
StyD4I CCNGG 2 cut(s) 163, 1253
TaaI ACNGT 4 cut(s) 327, 567, 1037, 1160
TaiI ACGT 2 cut(s) 1230, 1335
TaqI TCGA 8 cut(s) 5, 11, 246, 457, 512, 645, 930, 1397
TasI AATT 7 cut(s) 267, 448, 610, 634, 752, 1088, 1285
TatI WGTACW 1 cut(s) 567
TfiI GAWTC 3 cut(s) 74, 659, 1411
Tru1I TTAA 2 cut(s) 920, 1244
Tru9I TTAA 2 cut(s) 920, 1244
TscAI CASTG 1 cut(s) 940
TseFI GTSAC 2 cut(s) 305, 1223
TseI GCWGC 4 cut(s) 314, 702, 851, 962
Tsp45I GTSAC 2 cut(s) 305, 1223
TspRI CASTG 1 cut(s) 940
Tth111I GACNNNGTC 1 cut(s) 842
VpaK11BI GGWCC 2 cut(s) 44, 595
XagI CCTNNNNNAGG 2 cut(s) 644, 1097
XapI RAATTY 2 cut(s) 610, 1088
XcmI CCANNNNNNNNNTGG 1 cut(s) 497
XmnI GAANNNNTTC 2 cut(s) 436, 1022
XspI CTAG 7 cut(s) 33, 177, 378, 383, 575, 689, 1478
ZrmI AGTACT 1 cut(s) 569
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.