AT1G35850

Arabidopsis Pumilio

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009111
Physical Location & Seq
Forward (+)
13330510 .. 13331506
997 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G35850.1

Sequence Viewer

Length: 915 bp
ATGACAAACATCAATCGCTTATCAATGTCGACAATGTTCAACGCTTTACACGAAATCCTGAATGAGGAGCCTTTGGCAATTCCTCCTCCTCCTTCACGTGGCGGCTATTCCTACTTCCACATCCGTACAACGGAGATGGATTTGCAAAGGATGTTCAACTTCATGACTGGAAGCGAAGATTTGAAAGACGACATCTCAGTATTAGACACGGGAATGCTTAAACTCATGAGGATGTCAGATGACATGGACGTCTTCTTCTTTGTGGCTATCATGCGCCTCTTCATCCATGTCATGATCGACAAATACGCCTCCTACGTGGCGATACAGGGGATGAGAATTTTTAAACAGGACAAGAGAGAGTTGATGTATGATCACATTCTTCGATACGCGCTTTTCCTGGCGCGTGACCAGTATGGTTGCATTGCGCTCAACGAAATCATAAAAGAGTTGGACGATCCTTACTATAGAGACGAGCTCATGGACATAGTCTCAAACAACGCTCTTTTGCTAAGCAACGATGCTTATGGCAACTTTGTGGTCCAACACGTGCTTAAACTACATGACTCCCGTTGCACGGGTAACATTGCTGATAAACTATGTGGCTACTGCGTTGAGCTCTCGTTTAAAAAGTATGGAAGCTACATCGTGGAGCGACTTTTGGAGGTGAGGGACATACCAATGGCAACAATTGTTCTGGATCTTTTAGCGTGCAAGACAGAGATGTTGATAAGGTTGGCGAGGAGCGAGAACGGGAATTTTGTGGTGTGCAAATTACTGGAATTAACAAATGACATTCTCACTGCTGATCTATTTTACAGTTTGGTGAATAAGCTCAGGCCTTATCGCTTTCTCTTGCATAGGTTTCCTGAAAGCAAGATTGTAGCCATCTTGGGCTCAATGCGTGTCCCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

304

Amino Acids

35.27

Weight (kDa)

5.84

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01410 AT1G21620 AT1G35850 AT1G35850 AT5G59280 AT5G60110 AT5G60180 AT5G64490
fragaria_vesca FvH4_3g00680
malus_domestica MD05G1360200.v1.1
prunus_persica Prupe.4G007700_v2.0.a1
pyrus_communis pycom05g32520
rosa_chinensis RchiOBHm_Chr5g0000911
rosa_laevigata RLG00000030906
rosa_roxburghii Rroxscaffold_1G00075340
rosa_rugosa Rorug04G0387800 Rorug04G0387900.1
rosa_samantha Rh5AG007400 Rh5BG009500 Rh5CG008100 Rh5DG008100
rosa_wichuraiana Rw5G000770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AatII GACGTC 1 cut(s) 252
AccI GTMKAC 1 cut(s) 29
AccII CGCG 2 cut(s) 389, 403
AciI CCGC 1 cut(s) 102
AclWI GGATC 2 cut(s) 449, 705
AcsI RAATTY 2 cut(s) 336, 754
AcvI CACGTG 2 cut(s) 98, 547
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 1 cut(s) 127
AfiI CCNNNNNNNGG 4 cut(s) 64, 98, 130, 574
AflIII ACRYGT 1 cut(s) 544
AgsI TTSAA 3 cut(s) 40, 157, 184
AjnI CCWGG 1 cut(s) 396
AluBI AGCT 4 cut(s) 475, 616, 639, 832
AluI AGCT 4 cut(s) 475, 616, 639, 832
Alw21I GWGCWC 2 cut(s) 477, 618
Alw26I GTCTC 2 cut(s) 462, 493
AlwI GGATC 2 cut(s) 449, 705
AoxI GGCC 1 cut(s) 836
ApoI RAATTY 2 cut(s) 336, 754
AspLEI GCGC 4 cut(s) 276, 391, 403, 427
AspS9I GGNCC 1 cut(s) 538
AsuHPI GGTGA 2 cut(s) 676, 835
AvaII GGWCC 1 cut(s) 538
BanII GRGCYC 3 cut(s) 477, 618, 896
BbrPI CACGTG 2 cut(s) 98, 547
BbsI GAAGAC 1 cut(s) 244
Bbv12I GWGCWC 2 cut(s) 477, 618
BccI CCATC 2 cut(s) 130, 893
BciT130I CCWGG 1 cut(s) 398
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 2 cut(s) 462, 493
BfaI CTAG 1 cut(s) 913
BfmI CTRYAG 1 cut(s) 463
BisI GCNGC 1 cut(s) 103
BlpI GCTNAGC 1 cut(s) 509
BlsI GCNGC 1 cut(s) 104
Bme1390I CCNGG 1 cut(s) 398
Bme18I GGWCC 1 cut(s) 538
BmgT120I GGNCC 1 cut(s) 538
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 1 cut(s) 398
BmsI GCATC 1 cut(s) 508
BpiI GAAGAC 1 cut(s) 244
BplI GAGNNNNNCTC 2 cut(s) 459, 491
Bpu10I CCTNAGC 1 cut(s) 833
Bpu1102I GCTNAGC 1 cut(s) 509
BsaAI YACGTR 3 cut(s) 98, 316, 547
BsaHI GRCGYC 1 cut(s) 249
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 98, 130, 574
Bse1I ACTGG 3 cut(s) 172, 409, 780
Bse3DI GCAATG 2 cut(s) 420, 582
BseBI CCWGG 1 cut(s) 398
BseGI GGATG 5 cut(s) 120, 156, 237, 282, 336
BseLI CCNNNNNNNGG 4 cut(s) 64, 98, 130, 574
BseMI GCAATG 2 cut(s) 420, 582
BseMII CTCAG 2 cut(s) 210, 847
BseNI ACTGG 3 cut(s) 172, 409, 780
BseRI GAGGAG 4 cut(s) 75, 78, 80, 754
Bsh1236I CGCG 2 cut(s) 389, 403
BshFI GGCC 1 cut(s) 838
BsiHKAI GWGCWC 2 cut(s) 477, 618
BslFI GGGAC 2 cut(s) 683, 890
BslI CCNNNNNNNGG 4 cut(s) 64, 98, 130, 574
BsmAI GTCTC 2 cut(s) 462, 493
BsmBI CGTCTC 1 cut(s) 462
BsmFI GGGAC 2 cut(s) 683, 890
BsmI GAATGC 1 cut(s) 219
BsnI GGCC 1 cut(s) 838
Bsp1286I GDGCHC 3 cut(s) 477, 618, 896
Bsp143I GATC 5 cut(s) 294, 370, 454, 697, 805
Bsp1720I GCTNAGC 1 cut(s) 509
BspACI CCGC 1 cut(s) 102
BspANI GGCC 1 cut(s) 838
BspCNI CTCAG 2 cut(s) 209, 846
BspFNI CGCG 2 cut(s) 389, 403
BspHI TCATGA 3 cut(s) 162, 225, 291
BspLI GGNNCC 1 cut(s) 69
BspPI GGATC 2 cut(s) 449, 705
BsrDI GCAATG 2 cut(s) 420, 582
BsrI ACTGG 3 cut(s) 172, 409, 780
BssMI GATC 5 cut(s) 294, 370, 454, 697, 805
BssNI GRCGYC 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 1 cut(s) 818
Bst6I CTCTTC 1 cut(s) 284
BstACI GRCGYC 1 cut(s) 249
BstBAI YACGTR 3 cut(s) 98, 316, 547
BstC8I GCNNGC 1 cut(s) 709
BstDEI CTNAG 3 cut(s) 196, 509, 833
BstENI CCTNNNNNAGG 1 cut(s) 62
BstF5I GGATG 5 cut(s) 120, 156, 237, 282, 336
BstFNI CGCG 2 cut(s) 389, 403
BstHHI GCGC 4 cut(s) 276, 391, 403, 427
BstKTI GATC 5 cut(s) 297, 373, 457, 700, 808
BstMAI GTCTC 2 cut(s) 462, 493
BstMBI GATC 5 cut(s) 294, 370, 454, 697, 805
BstNI CCWGG 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 396
BstSFI CTRYAG 1 cut(s) 463
BstUI CGCG 2 cut(s) 389, 403
BstV2I GAAGAC 1 cut(s) 244
BstX2I RGATCY 1 cut(s) 697
BstYI RGATCY 1 cut(s) 697
BsuRI GGCC 1 cut(s) 838
BtsCI GGATG 5 cut(s) 120, 156, 237, 282, 336
BtsI GCAGTG 1 cut(s) 798
BtsIMutI CAGTG 1 cut(s) 798
Cac8I GCNNGC 1 cut(s) 709
CciI TCATGA 3 cut(s) 162, 225, 291
CfoI GCGC 4 cut(s) 276, 391, 403, 427
Cfr13I GGNCC 1 cut(s) 538
Csp6I GTAC 1 cut(s) 126
CviAII CATG 8 cut(s) 163, 226, 244, 271, 287, 292, 478, 560
CviQI GTAC 1 cut(s) 126
DdeI CTNAG 3 cut(s) 196, 509, 833
DpnI GATC 5 cut(s) 296, 372, 456, 699, 807
DpnII GATC 5 cut(s) 294, 370, 454, 697, 805
DraI TTTAAA 2 cut(s) 343, 625
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Ecl136II GAGCTC 2 cut(s) 475, 616
Eco147I AGGCCT 1 cut(s) 838
Eco24I GRGCYC 3 cut(s) 477, 618, 896
Eco47I GGWCC 1 cut(s) 538
Eco53kI GAGCTC 2 cut(s) 475, 616
Eco72I CACGTG 2 cut(s) 98, 547
EcoICRI GAGCTC 2 cut(s) 475, 616
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 396
EcoT38I GRGCYC 3 cut(s) 477, 618, 896
Esp3I CGTCTC 1 cut(s) 462
FaeI CATG 8 cut(s) 166, 229, 247, 274, 290, 295, 481, 563
FaqI GGGAC 2 cut(s) 683, 890
FatI CATG 8 cut(s) 162, 225, 243, 270, 286, 291, 477, 559
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 1 cut(s) 103
FokI GGATG 5 cut(s) 107, 163, 244, 269, 343
FriOI GRGCYC 3 cut(s) 477, 618, 896
Fsp4HI GCNGC 1 cut(s) 103
FspBI CTAG 1 cut(s) 913
GlaI GCGC 4 cut(s) 275, 390, 402, 426
GluI GCNGC 1 cut(s) 103
HaeIII GGCC 1 cut(s) 838
HhaI GCGC 4 cut(s) 276, 391, 403, 427
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 8 cut(s) 166, 229, 247, 274, 290, 295, 481, 563
Hin6I GCGC 4 cut(s) 274, 389, 401, 425
HinP1I GCGC 4 cut(s) 274, 389, 401, 425
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 1 cut(s) 563
HphI GGTGA 2 cut(s) 676, 835
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 1 cut(s) 238
Hpy188III TCNNGA 6 cut(s) 58, 163, 226, 292, 695, 866
Hpy8I GTNNAC 1 cut(s) 30
HpyAV CCTTC 1 cut(s) 102
HpyCH4III ACNGT 1 cut(s) 818
HpyCH4IV ACGT 4 cut(s) 97, 249, 315, 546
HpyCH4V TGCA 6 cut(s) 145, 420, 573, 711, 768, 856
HpyF3I CTNAG 3 cut(s) 196, 509, 833
HpySE526I ACGT 4 cut(s) 97, 249, 315, 546
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 8 cut(s) 166, 229, 247, 274, 290, 295, 481, 563
HspAI GCGC 4 cut(s) 274, 389, 401, 425
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 5 cut(s) 294, 370, 454, 697, 805
LmnI GCTCC 3 cut(s) 67, 649, 741
LweI GCATC 1 cut(s) 508
MaeI CTAG 1 cut(s) 913
MaeII ACGT 4 cut(s) 97, 249, 315, 546
MaeIII GTNAC 2 cut(s) 404, 578
MalI GATC 5 cut(s) 296, 372, 456, 699, 807
MboI GATC 5 cut(s) 294, 370, 454, 697, 805
MboII GAAGA 5 cut(s) 188, 244, 247, 271, 371
MfeI CAATTG 1 cut(s) 687
MflI RGATCY 1 cut(s) 697
MhlI GDGCHC 3 cut(s) 477, 618, 896
MluCI AATT 6 cut(s) 78, 336, 687, 754, 770, 779
MlyI GAGTC 1 cut(s) 557
MmeI TCCRAC 2 cut(s) 429, 565
MseI TTAA 5 cut(s) 219, 342, 552, 624, 782
MslI CAYNNNNRTG 3 cut(s) 212, 230, 677
MspR9I CCNGG 1 cut(s) 398
MunI CAATTG 1 cut(s) 687
Mva1269I GAATGC 1 cut(s) 219
MvaI CCWGG 1 cut(s) 398
MvnI CGCG 2 cut(s) 389, 403
NdeII GATC 5 cut(s) 294, 370, 454, 697, 805
NlaIII CATG 8 cut(s) 166, 229, 247, 274, 290, 295, 481, 563
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 404
PagI TCATGA 3 cut(s) 162, 225, 291
PceI AGGCCT 1 cut(s) 838
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 312
PctI GAATGC 1 cut(s) 219
PflFI GACNNNGTC 1 cut(s) 485
PkrI GCNGC 1 cut(s) 104
PleI GAGTC 1 cut(s) 557
PmaCI CACGTG 2 cut(s) 98, 547
PmlI CACGTG 2 cut(s) 98, 547
PpsI GAGTC 1 cut(s) 557
Ppu21I YACGTR 3 cut(s) 98, 316, 547
Psp124BI GAGCTC 2 cut(s) 477, 618
Psp6I CCWGG 1 cut(s) 396
PspCI CACGTG 2 cut(s) 98, 547
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 69
PspPI GGNCC 1 cut(s) 538
PsuI RGATCY 1 cut(s) 697
PsyI GACNNNGTC 1 cut(s) 485
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
RseI CAYNNNNRTG 3 cut(s) 212, 230, 677
SacI GAGCTC 2 cut(s) 477, 618
SalI GTCGAC 1 cut(s) 28
SaqAI TTAA 5 cut(s) 219, 342, 552, 624, 782
SatI GCNGC 1 cut(s) 103
Sau3AI GATC 5 cut(s) 294, 370, 454, 697, 805
Sau96I GGNCC 1 cut(s) 538
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 1 cut(s) 398
SduI GDGCHC 3 cut(s) 477, 618, 896
SfaNI GCATC 1 cut(s) 508
SfcI CTRYAG 1 cut(s) 463
SinI GGWCC 1 cut(s) 538
SmiMI CAYNNNNRTG 3 cut(s) 212, 230, 677
Sse9I AATT 6 cut(s) 78, 336, 687, 754, 770, 779
SseBI AGGCCT 1 cut(s) 838
SsiI CCGC 1 cut(s) 102
SspMI CTAG 1 cut(s) 913
SstI GAGCTC 2 cut(s) 477, 618
StuI AGGCCT 1 cut(s) 838
StyD4I CCNGG 1 cut(s) 396
TaaI ACNGT 1 cut(s) 818
TaiI ACGT 4 cut(s) 100, 252, 318, 549
TaqI TCGA 3 cut(s) 29, 297, 382
TasI AATT 6 cut(s) 78, 336, 687, 754, 770, 779
TauI GCSGC 1 cut(s) 105
Tru1I TTAA 5 cut(s) 219, 342, 552, 624, 782
Tru9I TTAA 5 cut(s) 219, 342, 552, 624, 782
TscAI CASTG 1 cut(s) 805
TseFI GTSAC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 2 cut(s) 151, 271
TspGWI ACGGA 2 cut(s) 113, 146
TspRI CASTG 1 cut(s) 805
Tth111I GACNNNGTC 1 cut(s) 485
VpaK11BI GGWCC 1 cut(s) 538
XagI CCTNNNNNAGG 1 cut(s) 62
XapI RAATTY 2 cut(s) 336, 754
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 1 cut(s) 913
ZraI GACGTC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.