Prupe.4G007700_v2.0.a1

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0009111
Physical Location & Seq
Forward (+)
401159 .. 404271
3113 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G007700.1

Sequence Viewer

Length: 1599 bp
ATGGGGACTTCATGTTACGCTGGTTCTTCTATTGAAGAAGAAGTAGAAAAAAGCCTTTGCCTTTCTTTGGGTAAGGTTCGGGTAAAGACGGACAAGTTTGAGAATGAGAAACCTGGTTTCTCTGTGCATAATGGTAACAATACTTGTTTGGGTTCGTCTTCTAATGCCTCTCCAAGCAAAACCCAACAAGCACAGCCTGATGTTTATCCCAAATACGAAAGCGGATTTGAAAATTCGCCTAGAGTTCCAGTGCCATTGTATCCACAAAGTATCTGGAGAGATCATATTTTGAACGGTGGAGGACACATTCTGGGTTCAAACAACCTGAATGGGCATGCCAATGCTTATCAACCAAAGAACCCTCTTGGTAACCTGACAGAAGAAACTACAAAATCAAGATTAAGTCGTGGAGCAACATCTCCATCTTTCTGCAACTGGGCAAACCAGCTCAACCCTTGTCTTAATAATTGGTACCAAAGTTCAGGAGAACAATATTACAGAAGCAATGAAAGGATTGGAATGTATTCAAATGGTGATCGGTACCCAAGTTCTGGAATATCTAGTTATATGGGGACTCAGTCAAGCTTTAACAATGATGGATGCTATGGTGATCAGATATTTAAGCTGCGGTCTTATGCCGATATAGCTCGACTCGCTATGACGCAACCCGGATCACAGAGCTTAATAAATATCATGGTGGCTTCTAAGGACCCATTCGCTAAGGATATGATTTTTACTGGGGTTTTCGGTTCCATATTCGAGGTGATGAATGACTCACATGGATACTATGTTTTTGGGAAGCTTATTGAATCATGCAATTACGGTCAGTTAAGACACATTGTAGCACTGATGACTTTGAATACTGAATCACTCGTCAGCATATCAACCAGAAAATTTGGATCAAAATCAATTCAGAGGCTTGTCAAGGTGCTTGAGAAATCACCCTTGATTTACACCTTGACATTAGCTCTATCCAGTGTATTGGGGCAACTGATGACCAACCGAACAGGGTCGTTTGTTATATTGAAGTGCTTAAACCTCCTTGACACACAGAAAAATGAGAAAATATACGAAACTGCTGAAAGGCTTTGCATCACTCTGGCCCAGAATGAAAAGGGATGCATATACTTGAATGAGTTCATCACCTACAGTAAAACTCCATACAGAGAGAGACTCATGAATGAAATCTCAAGCAAATCAAAACTCCTTTCTCAGGATCCTTCAGGGAACTTTGTTGTCCAGCACGTCCTGGGACTCCATAACCCTGTCTTCTCTGCAAAGATATGCTTTGAGCTGAGGGATCTGTACATAAAGCTCTCTTCACAAAGAGGTGGGAGTCATGTTGTAGAGAAATGCTTGAACTCTTCTGAGACAGATTATGTAGTAAATGCTTTTCTCAAGTATGAAAAACTGTCGCAAATTGCGCGGGATCAATTTGGAAACTACGTGATCCAAACAGCATTGAAGGCCACAAAGCGTGCAAATAGTCCCCTTTATCAAATGCTTTTAGCAAAGCTCGAACAAAATCGGAATGAACTCATGAGTGGATTTGGAAGGAAAGTGCTGAGTTTCATTGACAGTGGCGTCCCCCTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

533

Amino Acids

59.35

Weight (kDa)

8.86

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01410 AT1G21620 AT1G35850 AT1G35850 AT5G59280 AT5G60110 AT5G60180 AT5G64490
fragaria_vesca FvH4_3g00680
malus_domestica MD05G1360200.v1.1
prunus_persica Prupe.4G007700_v2.0.a1
pyrus_communis pycom05g32520
rosa_chinensis RchiOBHm_Chr5g0000911
rosa_laevigata RLG00000030906
rosa_roxburghii Rroxscaffold_1G00075340
rosa_rugosa Rorug04G0387800 Rorug04G0387900.1
rosa_samantha Rh5AG007400 Rh5BG009500 Rh5CG008100 Rh5DG008100
rosa_wichuraiana Rw5G000770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1583
Acc65I GGTACC 2 cut(s) 471, 540
AccB1I GGYRCC 2 cut(s) 471, 540
AccB7I CCANNNNNTGG 1 cut(s) 551
AccII CGCG 1 cut(s) 1426
AciI CCGC 3 cut(s) 222, 628, 1426
AclWI GGATC 7 cut(s) 679, 907, 1211, 1224, 1308, 1437, 1444
AcsI RAATTY 2 cut(s) 232, 893
AcuI CTGAAG 1 cut(s) 1206
AcyI GRCGYC 1 cut(s) 1584
AfaI GTAC 3 cut(s) 473, 542, 1307
AfiI CCNNNNNNNGG 3 cut(s) 67, 551, 1213
AjiI CACGTC 1 cut(s) 1246
AjnI CCWGG 2 cut(s) 112, 1248
Alw26I GTCTC 2 cut(s) 1165, 1364
AlwI GGATC 7 cut(s) 679, 907, 1211, 1224, 1308, 1437, 1444
AoxI GGCC 2 cut(s) 1101, 1467
ApeKI GCWGC 1 cut(s) 625
ApoI RAATTY 2 cut(s) 232, 893
Asp700I GAANNNNTTC 2 cut(s) 523, 1136
Asp718I GGTACC 2 cut(s) 471, 540
AspLEI GCGC 1 cut(s) 1426
AspS9I GGNCC 2 cut(s) 709, 1102
AsuC2I CCSGG 1 cut(s) 669
AsuHPI GGTGA 5 cut(s) 545, 620, 775, 933, 1135
AvaII GGWCC 1 cut(s) 709
BaeI ACNNNNGTAYC 4 cut(s) 463, 496, 532, 565
BamHI GGATCC 1 cut(s) 1216
BanI GGYRCC 2 cut(s) 471, 540
BbsI GAAGAC 2 cut(s) 150, 1261
BbvCI CCTCAGC 1 cut(s) 1295
BbvI GCAGC 1 cut(s) 612
BccI CCATC 2 cut(s) 430, 590
BciT130I CCWGG 2 cut(s) 114, 1250
BciVI GTATCC 2 cut(s) 270, 776
BclI TGATCA 1 cut(s) 610
BcnI CCSGG 1 cut(s) 669
BcoDI GTCTC 2 cut(s) 1165, 1364
BfaI CTAG 3 cut(s) 240, 561, 1592
BfmI CTRYAG 1 cut(s) 1147
BfuI GTATCC 2 cut(s) 270, 776
BisI GCNGC 1 cut(s) 626
BlsI GCNGC 1 cut(s) 627
Bme1390I CCNGG 3 cut(s) 114, 669, 1250
Bme18I GGWCC 1 cut(s) 709
BmgBI CACGTC 1 cut(s) 1246
BmgT120I GGNCC 2 cut(s) 709, 1102
BmiI GGNNCC 5 cut(s) 473, 542, 711, 751, 1218
BmrFI CCNGG 3 cut(s) 114, 669, 1250
BmrI ACTGGG 2 cut(s) 445, 747
BmsI GCATC 3 cut(s) 590, 1101, 1109
BmuI ACTGGG 2 cut(s) 445, 747
BpiI GAAGAC 2 cut(s) 150, 1261
BplI GAGNNNNNCTC 2 cut(s) 1158, 1190
BpmI CTGGAG 1 cut(s) 295
Bpu10I CCTNAGC 2 cut(s) 720, 1295
BpuEI CTTGAG 3 cut(s) 953, 1174, 1382
BpuMI CCSGG 1 cut(s) 669
BsaAI YACGTR 1 cut(s) 1447
BsaBI GATNNNNATC 2 cut(s) 204, 904
BsaHI GRCGYC 1 cut(s) 1584
BsaJI CCNNGG 1 cut(s) 1249
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 551, 1213
Bse1I ACTGG 4 cut(s) 248, 440, 742, 975
Bse3DI GCAATG 1 cut(s) 511
Bse8I GATNNNNATC 2 cut(s) 204, 904
BseBI CCWGG 2 cut(s) 114, 1250
BseDI CCNNGG 1 cut(s) 1249
BseGI GGATG 2 cut(s) 605, 1124
BseJI GATNNNNATC 2 cut(s) 204, 904
BseLI CCNNNNNNNGG 3 cut(s) 67, 551, 1213
BseMI GCAATG 1 cut(s) 511
BseMII CTCAG 5 cut(s) 590, 1226, 1286, 1359, 1556
BseNI ACTGG 4 cut(s) 248, 440, 742, 975
BseXI GCAGC 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 1426
BshFI GGCC 2 cut(s) 1103, 1469
BshNI GGYRCC 2 cut(s) 471, 540
BsiSI CCGG 1 cut(s) 669
BslFI GGGAC 5 cut(s) 19, 586, 1266, 1473, 1571
BslI CCNNNNNNNGG 3 cut(s) 67, 551, 1213
BsmAI GTCTC 2 cut(s) 1165, 1364
BsmFI GGGAC 5 cut(s) 19, 586, 1266, 1473, 1571
BsnI GGCC 2 cut(s) 1103, 1469
Bsp1407I TGTACA 1 cut(s) 1305
Bsp143I GATC 9 cut(s) 280, 535, 610, 671, 899, 1216, 1300, 1429, 1449
BspACI CCGC 3 cut(s) 222, 628, 1426
BspANI GGCC 2 cut(s) 1103, 1469
BspCNI CTCAG 5 cut(s) 589, 1225, 1287, 1360, 1557
BspFNI CGCG 1 cut(s) 1426
BspHI TCATGA 2 cut(s) 1176, 1539
BspLI GGNNCC 5 cut(s) 473, 542, 711, 751, 1218
BspPI GGATC 7 cut(s) 679, 907, 1211, 1224, 1308, 1437, 1444
BspT107I GGYRCC 2 cut(s) 471, 540
BsrDI GCAATG 1 cut(s) 511
BsrGI TGTACA 1 cut(s) 1305
BsrI ACTGG 4 cut(s) 248, 440, 742, 975
BssECI CCNNGG 1 cut(s) 1249
BssMI GATC 9 cut(s) 280, 535, 610, 671, 899, 1216, 1300, 1429, 1449
BssNI GRCGYC 1 cut(s) 1584
Bst2UI CCWGG 2 cut(s) 114, 1250
Bst4CI ACNGT 5 cut(s) 296, 824, 1151, 1413, 1580
Bst6I CTCTTC 2 cut(s) 1324, 1369
BstACI GRCGYC 1 cut(s) 1584
BstAUI TGTACA 1 cut(s) 1305
BstBAI YACGTR 1 cut(s) 1447
BstC8I GCNNGC 2 cut(s) 336, 1479
BstDEI CTNAG 7 cut(s) 576, 705, 720, 1212, 1295, 1368, 1565
BstEII GGTNACC 1 cut(s) 368
BstENI CCTNNNNNAGG 1 cut(s) 1211
BstF5I GGATG 2 cut(s) 605, 1124
BstFNI CGCG 1 cut(s) 1426
BstHHI GCGC 1 cut(s) 1426
BstKTI GATC 9 cut(s) 283, 538, 613, 674, 902, 1219, 1303, 1432, 1452
BstMAI GTCTC 2 cut(s) 1165, 1364
BstMBI GATC 9 cut(s) 280, 535, 610, 671, 899, 1216, 1300, 1429, 1449
BstMWI GCNNNNNNNGC 4 cut(s) 644, 653, 1423, 1466
BstNI CCWGG 2 cut(s) 114, 1250
BstNSI RCATGY 1 cut(s) 338
BstPI GGTNACC 1 cut(s) 368
BstSCI CCNGG 3 cut(s) 112, 667, 1248
BstSFI CTRYAG 1 cut(s) 1147
BstUI CGCG 1 cut(s) 1426
BstV1I GCAGC 1 cut(s) 612
BstV2I GAAGAC 2 cut(s) 150, 1261
BstX2I RGATCY 2 cut(s) 1216, 1300
BstXI CCANNNNNNTGG 1 cut(s) 982
BstYI RGATCY 2 cut(s) 1216, 1300
BsuI GTATCC 2 cut(s) 270, 776
BsuRI GGCC 2 cut(s) 1103, 1469
BtrI CACGTC 1 cut(s) 1246
BtsCI GGATG 2 cut(s) 605, 1124
BtsIMutI CAGTG 4 cut(s) 255, 845, 982, 1585
Cac8I GCNNGC 2 cut(s) 336, 1479
CciI TCATGA 2 cut(s) 1176, 1539
CfoI GCGC 1 cut(s) 1426
Cfr13I GGNCC 2 cut(s) 709, 1102
CseI GACGC 2 cut(s) 670, 1573
CsiI ACCWGGT 1 cut(s) 112
Csp6I GTAC 3 cut(s) 472, 541, 1306
CviAII CATG 8 cut(s) 12, 335, 694, 779, 813, 1177, 1340, 1540
CviQI GTAC 3 cut(s) 472, 541, 1306
DdeI CTNAG 7 cut(s) 576, 705, 720, 1212, 1295, 1368, 1565
DpnI GATC 9 cut(s) 282, 537, 612, 673, 901, 1218, 1302, 1431, 1451
DpnII GATC 9 cut(s) 280, 535, 610, 671, 899, 1216, 1300, 1429, 1449
DrdI GACNNNNNNGTC 1 cut(s) 1583
DseDI GACNNNNNNGTC 1 cut(s) 1583
Eam1104I CTCTTC 2 cut(s) 1324, 1369
EarI CTCTTC 2 cut(s) 1324, 1369
Eco47I GGWCC 1 cut(s) 709
Eco57I CTGAAG 1 cut(s) 1206
Eco91I GGTNACC 1 cut(s) 368
EcoNI CCTNNNNNAGG 1 cut(s) 1211
EcoO109I RGGNCCY 1 cut(s) 709
EcoO65I GGTNACC 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 112, 1248
EcoT22I ATGCAT 1 cut(s) 1124
FaeI CATG 8 cut(s) 15, 338, 697, 782, 816, 1180, 1343, 1543
FalI AAGNNNNNCTT 2 cut(s) 1271, 1303
FaqI GGGAC 5 cut(s) 19, 586, 1266, 1473, 1571
FatI CATG 8 cut(s) 11, 334, 693, 778, 812, 1176, 1339, 1539
FauI CCCGC 1 cut(s) 1419
FbaI TGATCA 1 cut(s) 610
Fnu4HI GCNGC 1 cut(s) 626
FokI GGATG 2 cut(s) 612, 1131
Fsp4HI GCNGC 1 cut(s) 626
FspBI CTAG 3 cut(s) 240, 561, 1592
GlaI GCGC 1 cut(s) 1425
GluI GCNGC 1 cut(s) 626
GsuI CTGGAG 1 cut(s) 295
HaeIII GGCC 2 cut(s) 1103, 1469
HapII CCGG 1 cut(s) 669
HgaI GACGC 2 cut(s) 670, 1573
HhaI GCGC 1 cut(s) 1426
Hin1I GRCGYC 1 cut(s) 1584
Hin1II CATG 8 cut(s) 15, 338, 697, 782, 816, 1180, 1343, 1543
Hin6I GCGC 1 cut(s) 1424
HinP1I GCGC 1 cut(s) 1424
HindIII AAGCTT 2 cut(s) 583, 800
HinfI GANTC 8 cut(s) 574, 651, 773, 809, 866, 1173, 1254, 1336
HpaII CCGG 1 cut(s) 669
HphI GGTGA 5 cut(s) 545, 620, 775, 933, 1135
Hpy188I TCNGA 4 cut(s) 615, 915, 1369, 1530
Hpy188III TCNNGA 7 cut(s) 274, 396, 483, 552, 1177, 1214, 1540
HpyAV CCTTC 3 cut(s) 1230, 1459, 1548
HpyCH4III ACNGT 5 cut(s) 296, 824, 1151, 1413, 1580
HpyCH4IV ACGT 2 cut(s) 1245, 1446
HpyCH4V TGCA 7 cut(s) 127, 432, 816, 1092, 1122, 1277, 1481
HpyF10VI GCNNNNNNNGC 4 cut(s) 644, 653, 1423, 1466
HpyF3I CTNAG 7 cut(s) 576, 705, 720, 1212, 1295, 1368, 1565
HpySE526I ACGT 2 cut(s) 1245, 1446
Hsp92I GRCGYC 1 cut(s) 1584
Hsp92II CATG 8 cut(s) 15, 338, 697, 782, 816, 1180, 1343, 1543
HspAI GCGC 1 cut(s) 1424
KpnI GGTACC 2 cut(s) 475, 544
Ksp22I TGATCA 1 cut(s) 610
Kzo9I GATC 9 cut(s) 280, 535, 610, 671, 899, 1216, 1300, 1429, 1449
LmnI GCTCC 1 cut(s) 410
Lsp1109I GCAGC 1 cut(s) 612
LweI GCATC 3 cut(s) 590, 1101, 1109
MabI ACCWGGT 1 cut(s) 112
MaeI CTAG 3 cut(s) 240, 561, 1592
MaeII ACGT 2 cut(s) 1245, 1446
MaeIII GTNAC 3 cut(s) 14, 134, 368
MalI GATC 9 cut(s) 282, 537, 612, 673, 901, 1218, 1302, 1431, 1451
MboI GATC 9 cut(s) 280, 535, 610, 671, 899, 1216, 1300, 1429, 1449
MboII GAAGA 8 cut(s) 18, 47, 50, 150, 392, 1261, 1311, 1356
MflI RGATCY 2 cut(s) 1216, 1300
MluCI AATT 7 cut(s) 232, 466, 817, 893, 909, 1419, 1433
MlyI GAGTC 6 cut(s) 568, 645, 767, 1167, 1248, 1345
MnlI CCTC 8 cut(s) 178, 293, 372, 754, 909, 1049, 1290, 1322
Mph1103I ATGCAT 1 cut(s) 1124
MroXI GAANNNNTTC 2 cut(s) 523, 1136
MseI TTAA 7 cut(s) 401, 462, 588, 621, 683, 830, 1034
MslI CAYNNNNRTG 1 cut(s) 339
MspI CCGG 1 cut(s) 669
MspR9I CCNGG 3 cut(s) 114, 669, 1250
MvaI CCWGG 2 cut(s) 114, 1250
MvnI CGCG 1 cut(s) 1426
MwoI GCNNNNNNNGC 4 cut(s) 644, 653, 1423, 1466
NciI CCSGG 1 cut(s) 669
NdeII GATC 9 cut(s) 280, 535, 610, 671, 899, 1216, 1300, 1429, 1449
NlaIII CATG 8 cut(s) 15, 338, 697, 782, 816, 1180, 1343, 1543
NlaIV GGNNCC 5 cut(s) 473, 542, 711, 751, 1218
NsiI ATGCAT 1 cut(s) 1124
NspI RCATGY 1 cut(s) 338
PaeI GCATGC 1 cut(s) 338
PagI TCATGA 2 cut(s) 1176, 1539
PdmI GAANNNNTTC 2 cut(s) 523, 1136
PfeI GAWTC 2 cut(s) 809, 866
PflFI GACNNNGTC 1 cut(s) 577
PflMI CCANNNNNTGG 1 cut(s) 551
PkrI GCNGC 1 cut(s) 627
PleI GAGTC 6 cut(s) 568, 645, 767, 1167, 1248, 1344
PpsI GAGTC 6 cut(s) 568, 645, 767, 1167, 1248, 1344
Ppu21I YACGTR 1 cut(s) 1447
PpuMI RGGWCCY 1 cut(s) 709
Psp5II RGGWCCY 1 cut(s) 709
Psp6I CCWGG 2 cut(s) 112, 1248
PspEI GGTNACC 1 cut(s) 368
PspGI CCWGG 2 cut(s) 112, 1248
PspN4I GGNNCC 5 cut(s) 473, 542, 711, 751, 1218
PspPI GGNCC 2 cut(s) 709, 1102
PspPPI RGGWCCY 1 cut(s) 709
PsuI RGATCY 2 cut(s) 1216, 1300
PsyI GACNNNGTC 1 cut(s) 577
RsaI GTAC 3 cut(s) 473, 542, 1307
RsaNI GTAC 3 cut(s) 472, 541, 1306
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 7 cut(s) 401, 462, 588, 621, 683, 830, 1034
SatI GCNGC 1 cut(s) 626
Sau3AI GATC 9 cut(s) 280, 535, 610, 671, 899, 1216, 1300, 1429, 1449
Sau96I GGNCC 2 cut(s) 709, 1102
SchI GAGTC 6 cut(s) 568, 645, 767, 1167, 1248, 1345
ScrFI CCNGG 3 cut(s) 114, 669, 1250
SexAI ACCWGGT 1 cut(s) 112
SfaNI GCATC 3 cut(s) 590, 1101, 1109
SfcI CTRYAG 1 cut(s) 1147
SinI GGWCC 1 cut(s) 709
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 3 cut(s) 932, 1189, 1397
SmoI CTYRAG 3 cut(s) 932, 1189, 1397
SphI GCATGC 1 cut(s) 338
Sse9I AATT 7 cut(s) 232, 466, 817, 893, 909, 1419, 1433
SsiI CCGC 3 cut(s) 222, 628, 1426
SspI AATATT 1 cut(s) 494
SspMI CTAG 3 cut(s) 240, 561, 1592
StyD4I CCNGG 3 cut(s) 112, 667, 1248
TaaI ACNGT 5 cut(s) 296, 824, 1151, 1413, 1580
TaiI ACGT 2 cut(s) 1248, 1449
TaqI TCGA 3 cut(s) 649, 759, 1518
TasI AATT 7 cut(s) 232, 466, 817, 893, 909, 1419, 1433
TatI WGTACW 1 cut(s) 1305
TfiI GAWTC 2 cut(s) 809, 866
Tru1I TTAA 7 cut(s) 401, 462, 588, 621, 683, 830, 1034
Tru9I TTAA 7 cut(s) 401, 462, 588, 621, 683, 830, 1034
TscAI CASTG 4 cut(s) 255, 852, 982, 1585
TseI GCWGC 1 cut(s) 625
TspDTI ATGAA 9 cut(s) 522, 782, 1125, 1129, 1193, 1197, 1419, 1548, 1561
TspGWI ACGGA 1 cut(s) 104
TspRI CASTG 4 cut(s) 255, 852, 982, 1585
Tth111I GACNNNGTC 1 cut(s) 577
Van91I CCANNNNNTGG 1 cut(s) 551
VpaK11BI GGWCC 1 cut(s) 709
XagI CCTNNNNNAGG 1 cut(s) 1211
XapI RAATTY 2 cut(s) 232, 893
XceI RCATGY 1 cut(s) 338
XcmI CCANNNNNNNNNTGG 1 cut(s) 270
XmnI GAANNNNTTC 2 cut(s) 523, 1136
XspI CTAG 3 cut(s) 240, 561, 1592
Zsp2I ATGCAT 1 cut(s) 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.