MD05G1360200.v1.1

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009111
Physical Location & Seq
Reverse (-)
47480374 .. 47482663
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1360200.v1.1.491

Sequence Viewer

Length: 1524 bp
ATGGCGACTTCATGTTACTCTGCTTCCGACCCTCCCAATCCAGAGACTCCTTGCGTGCCTCCTTCTTACTCTCTTCGACGACAACAAGAAGCCGACCTCTGCCATTATTTCGGCAACGTTCGGGTAACAGTGGACAAAAGCAGCACTAGTTCTCCGGGTTCGTCTTCATATGCTACTCCCACCAAAATCGAACAAGCACAATATGATTTTTATCCGAGATACGAAAGCGGGTTAAGAAATTGTCCGAGAGCTCCAATGGCATTGTATTCAGAGAGTATATGGGGAGGCAATAGTGTGAACGCTAGAGGAAACTTTATGGGTTCAAACAATCTGAATGTCCATCAACTTATTGGCAATCCGGCTACAGAAGAAAGGACAAGAGCAAATTGGAGTGTTGGAGCAACATCTACATCTTTGGGCAATTGGGCACACCAGCTTAGCCCTTTTGGTAATAGCGATTGGAACCAAAATTATGGAGGACAAAATTACAGAGGCAATGTTGAAAGCACTGGATTATATTATTTCAAAGGTAATAATTGGGCCCAAAGTTCAGGAATACATAGTGAGATAGCTCGACTCGCTATGACTCAACTCGGATCGCAGGCCTTGGTACAGAGAATGATAACTCCCAGCGACCCATTAGCAAAGAGTATGGTACTCGAAGGGGTTTTTGGTTCCTTATTCGAGGTGATGTGTGACTTTCATGGACACTATGTTTTTCGGAAGCTTGTTGAATCATGCAATCACAGTCAGTTGAGACTCATTGTGGCTAAGATAACTTTGAATTCTAAACCACTCATCAACATATCAGTCTGCAATTATGGCTCAAAATCAATTCAAATGCTGATCAAGGTGCTCGAGAAATCGCCCCTGATTTACACTTTGACATCAGCTCTATCTGATGTTGTCGAAGAACTGATGACCAATCGTACAGGATCCTATGTCATCTTGAAGTGCTTAAACCAACTTGACATTGAGAAAAATAAGAAAATATATGAAGCTGTTGTGAGGCTTTGCGTCCGATTGGCTCAGCACGAAAGGGGATGCATACACTTGAATGAGTTCATCACCAACAGTAAAACTCCATACAGAGAGAAACTCATGGACAAAGTCTCGAGCAACTCAAAATTCCTTTCTCAGGATCCTTCGGGGAACTTTGTTGTCCAGCACATCTTCACGCTATATAACCCAGTCTTCTCTGCAAAGATATGCTTTGAGCTTCGAAATCTTTACATACAACTCTCATTGCAAAAAGGCGGGAGTCACGTTGTAGAGATGTGCTTAAACTCTTTGGGAATGGAAGTTGTAGTGAATGTTTTTCTAAAGTATGACAAACTCTGGCAAGTTGCGCGAGATCAGTATGGAAACTACGTGATCCAAACAGCATTGAAGGCCACAAAGCGCGAAAATAGCCCCCTTCATCAAATGCTTCTAGAAAAGCTCAACAAAGATCGAAGTGAACTCATGGTTGGGTTTGGAAGGAAAGTGCTGAGCGTCATTGACAAAGGGATCCCCCTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

508

Amino Acids

56.89

Weight (kDa)

8.9

Isoelectric Point (pI)

33.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01410 AT1G21620 AT1G35850 AT1G35850 AT5G59280 AT5G60110 AT5G60180 AT5G64490
fragaria_vesca FvH4_3g00680
malus_domestica MD05G1360200.v1.1
prunus_persica Prupe.4G007700_v2.0.a1
pyrus_communis pycom05g32520
rosa_chinensis RchiOBHm_Chr5g0000911
rosa_laevigata RLG00000030906
rosa_roxburghii Rroxscaffold_1G00075340
rosa_rugosa Rorug04G0387800 Rorug04G0387900.1
rosa_samantha Rh5AG007400 Rh5BG009500 Rh5CG008100 Rh5DG008100
rosa_wichuraiana Rw5G000770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 1351, 1404
AciI CCGC 2 cut(s) 228, 1257
AclI AACGTT 1 cut(s) 117
AclWI GGATC 8 cut(s) 604, 930, 943, 1136, 1149, 1369, 1504, 1517
AcsI RAATTY 2 cut(s) 784, 1127
AfaI GTAC 3 cut(s) 612, 657, 931
AfiI CCNNNNNNNGG 1 cut(s) 1138
AgsI TTSAA 9 cut(s) 324, 503, 526, 734, 784, 839, 952, 1057, 1390
AhlI ACTAGT 1 cut(s) 146
AjuI GAANNNNNNNTTGG 4 cut(s) 654, 686, 1452, 1484
AluBI AGCT 8 cut(s) 251, 436, 572, 727, 893, 1001, 1219, 1441
AluI AGCT 8 cut(s) 251, 436, 572, 727, 893, 1001, 1219, 1441
Alw21I GWGCWC 2 cut(s) 253, 858
Alw26I GTCTC 3 cut(s) 38, 751, 1117
AlwI GGATC 8 cut(s) 604, 930, 943, 1136, 1149, 1369, 1504, 1517
Ama87I CYCGRG 2 cut(s) 857, 1114
AoxI GGCC 3 cut(s) 540, 603, 1392
ApaI GGGCCC 1 cut(s) 544
ApeKI GCWGC 1 cut(s) 141
ApoI RAATTY 2 cut(s) 784, 1127
Asp700I GAANNNNTTC 1 cut(s) 1061
AspLEI GCGC 2 cut(s) 1351, 1404
AspS9I GGNCC 2 cut(s) 540, 541
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 2 cut(s) 700, 1060
AsuII TTCGAA 1 cut(s) 1222
AvaI CYCGRG 2 cut(s) 857, 1114
BaeGI GKGCMC 2 cut(s) 430, 544
BamHI GGATCC 3 cut(s) 935, 1141, 1509
BanII GRGCYC 2 cut(s) 253, 544
BbsI GAAGAC 2 cut(s) 156, 1186
Bbv12I GWGCWC 2 cut(s) 253, 858
BbvI GCAGC 1 cut(s) 153
BccI CCATC 1 cut(s) 348
BclI TGATCA 1 cut(s) 846
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 3 cut(s) 38, 751, 1117
BcuI ACTAGT 1 cut(s) 146
BfaI CTAG 4 cut(s) 147, 303, 1433, 1517
BfmI CTRYAG 1 cut(s) 363
BisI GCNGC 1 cut(s) 142
BlpI GCTNAGC 3 cut(s) 437, 1029, 1490
BlsI GCNGC 1 cut(s) 143
Bme1390I CCNGG 1 cut(s) 156
BmeT110I CYCGRG 2 cut(s) 857, 1114
BmgT120I GGNCC 2 cut(s) 540, 541
BmiI GGNNCC 6 cut(s) 464, 542, 676, 937, 1143, 1511
BmrFI CCNGG 1 cut(s) 156
BmrI ACTGGG 1 cut(s) 1184
BmsI GCATC 1 cut(s) 1034
BmuI ACTGGG 1 cut(s) 1184
BpiI GAAGAC 2 cut(s) 156, 1186
BplI GAGNNNNNCTC 2 cut(s) 1083, 1115
Bpu1102I GCTNAGC 3 cut(s) 437, 1029, 1490
Bpu14I TTCGAA 1 cut(s) 1222
BpuMI CCSGG 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 1372
BsaBI GATNNNNATC 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 606
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bsc4I CCNNNNNNNGG 1 cut(s) 1138
Bse1I ACTGG 2 cut(s) 514, 1190
Bse3DI GCAATG 2 cut(s) 502, 1244
Bse8I GATNNNNATC 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 606
BseGI GGATG 1 cut(s) 1049
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 1138
BseMI GCAATG 2 cut(s) 502, 1244
BseMII CTCAG 3 cut(s) 1043, 1151, 1481
BseNI ACTGG 2 cut(s) 514, 1190
BseSI GKGCMC 2 cut(s) 430, 544
BseXI GCAGC 1 cut(s) 153
BseYI CCCAGC 1 cut(s) 629
Bsh1236I CGCG 2 cut(s) 1351, 1404
BshFI GGCC 3 cut(s) 542, 605, 1394
BsiHKAI GWGCWC 2 cut(s) 253, 858
BsiHKCI CYCGRG 2 cut(s) 857, 1114
BsiSI CCGG 2 cut(s) 155, 359
BslI CCNNNNNNNGG 1 cut(s) 1138
BsmAI GTCTC 3 cut(s) 38, 751, 1117
BsnI GGCC 3 cut(s) 542, 605, 1394
BsoBI CYCGRG 2 cut(s) 857, 1114
Bsp119I TTCGAA 1 cut(s) 1222
Bsp120I GGGCCC 1 cut(s) 540
Bsp1286I GDGCHC 4 cut(s) 253, 430, 544, 858
Bsp143I GATC 8 cut(s) 596, 846, 935, 1141, 1354, 1374, 1450, 1509
Bsp1720I GCTNAGC 3 cut(s) 437, 1029, 1490
BspACI CCGC 2 cut(s) 228, 1257
BspANI GGCC 3 cut(s) 542, 605, 1394
BspCNI CTCAG 3 cut(s) 1042, 1150, 1482
BspFNI CGCG 2 cut(s) 1351, 1404
BspLI GGNNCC 6 cut(s) 464, 542, 676, 937, 1143, 1511
BspPI GGATC 8 cut(s) 604, 930, 943, 1136, 1149, 1369, 1504, 1517
BspT104I TTCGAA 1 cut(s) 1222
BsrDI GCAATG 2 cut(s) 502, 1244
BsrI ACTGG 2 cut(s) 514, 1190
BssECI CCNNGG 1 cut(s) 606
BssMI GATC 8 cut(s) 596, 846, 935, 1141, 1354, 1374, 1450, 1509
BssT1I CCWWGG 1 cut(s) 606
Bst4CI ACNGT 3 cut(s) 130, 749, 1076
Bst6I CTCTTC 1 cut(s) 78
BstBAI YACGTR 1 cut(s) 1372
BstBI TTCGAA 1 cut(s) 1222
BstC8I GCNNGC 2 cut(s) 56, 603
BstDEI CTNAG 5 cut(s) 437, 771, 1029, 1137, 1490
BstENI CCTNNNNNAGG 1 cut(s) 1136
BstF5I GGATG 1 cut(s) 1049
BstFNI CGCG 2 cut(s) 1351, 1404
BstHHI GCGC 2 cut(s) 1351, 1404
BstKTI GATC 8 cut(s) 599, 849, 938, 1144, 1357, 1377, 1453, 1512
BstMAI GTCTC 3 cut(s) 38, 751, 1117
BstMBI GATC 8 cut(s) 596, 846, 935, 1141, 1354, 1374, 1450, 1509
BstMWI GCNNNNNNNGC 6 cut(s) 257, 578, 822, 1348, 1391, 1410
BstSCI CCNGG 1 cut(s) 154
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 2 cut(s) 430, 544
BstUI CGCG 2 cut(s) 1351, 1404
BstV1I GCAGC 1 cut(s) 153
BstV2I GAAGAC 2 cut(s) 156, 1186
BstX2I RGATCY 3 cut(s) 935, 1141, 1509
BstXI CCANNNNNNTGG 1 cut(s) 473
BstYI RGATCY 3 cut(s) 935, 1141, 1509
BsuRI GGCC 3 cut(s) 542, 605, 1394
BtsCI GGATG 1 cut(s) 1049
BtsIMutI CAGTG 2 cut(s) 135, 507
Cac8I GCNNGC 2 cut(s) 56, 603
CfoI GCGC 2 cut(s) 1351, 1404
Cfr13I GGNCC 2 cut(s) 540, 541
CseI GACGC 2 cut(s) 1006, 1483
Csp6I GTAC 3 cut(s) 611, 656, 930
CviAII CATG 5 cut(s) 12, 704, 738, 1102, 1465
CviQI GTAC 3 cut(s) 611, 656, 930
DdeI CTNAG 5 cut(s) 437, 771, 1029, 1137, 1490
DpnI GATC 8 cut(s) 598, 848, 937, 1143, 1356, 1376, 1452, 1511
DpnII GATC 8 cut(s) 596, 846, 935, 1141, 1354, 1374, 1450, 1509
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Ecl136II GAGCTC 1 cut(s) 251
Eco130I CCWWGG 1 cut(s) 606
Eco147I AGGCCT 1 cut(s) 605
Eco24I GRGCYC 2 cut(s) 253, 544
Eco53kI GAGCTC 1 cut(s) 251
Eco88I CYCGRG 2 cut(s) 857, 1114
EcoICRI GAGCTC 1 cut(s) 251
EcoNI CCTNNNNNAGG 1 cut(s) 1136
EcoRI GAATTC 1 cut(s) 784
EcoT14I CCWWGG 1 cut(s) 606
EcoT22I ATGCAT 1 cut(s) 1049
EcoT38I GRGCYC 2 cut(s) 253, 544
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 5 cut(s) 15, 707, 741, 1105, 1468
FalI AAGNNNNNCTT 2 cut(s) 1196, 1228
FatI CATG 5 cut(s) 11, 703, 737, 1101, 1464
FauI CCCGC 2 cut(s) 221, 1250
FauNDI CATATG 1 cut(s) 169
FbaI TGATCA 1 cut(s) 846
Fnu4HI GCNGC 1 cut(s) 142
FokI GGATG 1 cut(s) 1056
FriOI GRGCYC 2 cut(s) 253, 544
Fsp4HI GCNGC 1 cut(s) 142
FspBI CTAG 4 cut(s) 147, 303, 1433, 1517
GlaI GCGC 2 cut(s) 1350, 1403
GluI GCNGC 1 cut(s) 142
GsaI CCCAGC 1 cut(s) 633
HaeIII GGCC 3 cut(s) 542, 605, 1394
HapII CCGG 2 cut(s) 155, 359
HgaI GACGC 2 cut(s) 1006, 1483
HhaI GCGC 2 cut(s) 1351, 1404
Hin1II CATG 5 cut(s) 15, 707, 741, 1105, 1468
Hin6I GCGC 2 cut(s) 1349, 1402
HinP1I GCGC 2 cut(s) 1349, 1402
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 6 cut(s) 46, 576, 586, 734, 759, 1261
HpaII CCGG 2 cut(s) 155, 359
HphI GGTGA 2 cut(s) 700, 1060
Hpy166II GTNNAC 3 cut(s) 133, 298, 1460
Hpy188I TCNGA 9 cut(s) 28, 216, 246, 271, 333, 596, 723, 901, 1022
Hpy188III TCNNGA 7 cut(s) 41, 552, 859, 949, 1114, 1139, 1433
Hpy8I GTNNAC 3 cut(s) 133, 298, 1460
Hpy99I CGWCG 1 cut(s) 81
HpyAV CCTTC 6 cut(s) 72, 656, 1155, 1384, 1427, 1473
HpyCH4III ACNGT 3 cut(s) 130, 749, 1076
HpyCH4IV ACGT 3 cut(s) 117, 1266, 1371
HpyCH4V TGCA 5 cut(s) 741, 816, 1047, 1202, 1249
HpyF10VI GCNNNNNNNGC 6 cut(s) 257, 578, 822, 1348, 1391, 1410
HpyF3I CTNAG 5 cut(s) 437, 771, 1029, 1137, 1490
HpySE526I ACGT 3 cut(s) 117, 1266, 1371
Hsp92II CATG 5 cut(s) 15, 707, 741, 1105, 1468
HspAI GCGC 2 cut(s) 1349, 1402
Ksp22I TGATCA 1 cut(s) 846
Kzo9I GATC 8 cut(s) 596, 846, 935, 1141, 1354, 1374, 1450, 1509
LmnI GCTCC 2 cut(s) 256, 398
Lsp1109I GCAGC 1 cut(s) 153
LweI GCATC 1 cut(s) 1034
MaeI CTAG 4 cut(s) 147, 303, 1433, 1517
MaeII ACGT 3 cut(s) 117, 1266, 1371
MaeIII GTNAC 4 cut(s) 14, 124, 695, 1262
MalI GATC 8 cut(s) 598, 848, 937, 1143, 1356, 1376, 1452, 1511
MboI GATC 8 cut(s) 596, 846, 935, 1141, 1354, 1374, 1450, 1509
MboII GAAGA 6 cut(s) 65, 156, 380, 923, 1165, 1186
MfeI CAATTG 1 cut(s) 421
MflI RGATCY 3 cut(s) 935, 1141, 1509
MhlI GDGCHC 4 cut(s) 253, 430, 544, 858
MlyI GAGTC 5 cut(s) 40, 570, 580, 753, 1270
MmeI TCCRAC 2 cut(s) 51, 376
MnlI CCTC 9 cut(s) 42, 69, 107, 278, 299, 470, 485, 679, 1002
Mph1103I ATGCAT 1 cut(s) 1049
MroXI GAANNNNTTC 1 cut(s) 1061
MseI TTAA 3 cut(s) 233, 959, 1283
MslI CAYNNNNRTG 1 cut(s) 1056
MspI CCGG 2 cut(s) 155, 359
MspR9I CCNGG 1 cut(s) 156
MunI CAATTG 1 cut(s) 421
MvnI CGCG 2 cut(s) 1351, 1404
MwoI GCNNNNNNNGC 6 cut(s) 257, 578, 822, 1348, 1391, 1410
NciI CCSGG 1 cut(s) 156
NdeI CATATG 1 cut(s) 169
NdeII GATC 8 cut(s) 596, 846, 935, 1141, 1354, 1374, 1450, 1509
NlaIII CATG 5 cut(s) 15, 707, 741, 1105, 1468
NlaIV GGNNCC 6 cut(s) 464, 542, 676, 937, 1143, 1511
NmuCI GTSAC 2 cut(s) 695, 1262
NsiI ATGCAT 1 cut(s) 1049
NspV TTCGAA 1 cut(s) 1222
PaeR7I CTCGAG 2 cut(s) 857, 1114
PceI AGGCCT 1 cut(s) 605
PdmI GAANNNNTTC 1 cut(s) 1061
PfeI GAWTC 1 cut(s) 734
PflFI GACNNNGTC 1 cut(s) 1109
PkrI GCNGC 1 cut(s) 143
PleI GAGTC 5 cut(s) 40, 570, 580, 753, 1269
PpsI GAGTC 5 cut(s) 40, 570, 580, 753, 1269
Ppu21I YACGTR 1 cut(s) 1372
Psp124BI GAGCTC 1 cut(s) 253
Psp1406I AACGTT 1 cut(s) 117
PspFI CCCAGC 1 cut(s) 629
PspN4I GGNNCC 6 cut(s) 464, 542, 676, 937, 1143, 1511
PspOMI GGGCCC 1 cut(s) 540
PspPI GGNCC 2 cut(s) 540, 541
PsuI RGATCY 3 cut(s) 935, 1141, 1509
PsyI GACNNNGTC 1 cut(s) 1109
RsaI GTAC 3 cut(s) 612, 657, 931
RsaNI GTAC 3 cut(s) 611, 656, 930
RseI CAYNNNNRTG 1 cut(s) 1056
SacI GAGCTC 1 cut(s) 253
SaqAI TTAA 3 cut(s) 233, 959, 1283
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 8 cut(s) 596, 846, 935, 1141, 1354, 1374, 1450, 1509
Sau96I GGNCC 2 cut(s) 540, 541
SchI GAGTC 5 cut(s) 40, 570, 580, 753, 1270
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 4 cut(s) 253, 430, 544, 858
SfaNI GCATC 1 cut(s) 1034
SfcI CTRYAG 1 cut(s) 363
Sfr274I CTCGAG 2 cut(s) 857, 1114
SfuI TTCGAA 1 cut(s) 1222
SlaI CTCGAG 2 cut(s) 857, 1114
SmiMI CAYNNNNRTG 1 cut(s) 1056
SmlI CTYRAG 2 cut(s) 857, 1114
SmoI CTYRAG 2 cut(s) 857, 1114
SpeI ACTAGT 1 cut(s) 146
SseBI AGGCCT 1 cut(s) 605
SsiI CCGC 2 cut(s) 228, 1257
SspMI CTAG 4 cut(s) 147, 303, 1433, 1517
SstI GAGCTC 1 cut(s) 253
StuI AGGCCT 1 cut(s) 605
StyD4I CCNGG 1 cut(s) 154
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 3 cut(s) 130, 749, 1076
TaiI ACGT 3 cut(s) 120, 1269, 1374
TfiI GAWTC 1 cut(s) 734
Tru1I TTAA 3 cut(s) 233, 959, 1283
Tru9I TTAA 3 cut(s) 233, 959, 1283
TscAI CASTG 2 cut(s) 135, 514
TseFI GTSAC 2 cut(s) 695, 1262
TseI GCWGC 1 cut(s) 141
Tsp45I GTSAC 2 cut(s) 695, 1262
TspDTI ATGAA 5 cut(s) 156, 692, 1011, 1054, 1409
TspRI CASTG 2 cut(s) 135, 514
Tth111I GACNNNGTC 1 cut(s) 1109
XagI CCTNNNNNAGG 1 cut(s) 1136
XapI RAATTY 2 cut(s) 784, 1127
XbaI TCTAGA 1 cut(s) 1432
XcmI CCANNNNNNNNNTGG 1 cut(s) 347
XhoI CTCGAG 2 cut(s) 857, 1114
XmnI GAANNNNTTC 1 cut(s) 1061
XspI CTAG 4 cut(s) 147, 303, 1433, 1517
Zsp2I ATGCAT 1 cut(s) 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.