pycom05g32520

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009111
Physical Location & Seq
Reverse (-)
31901631 .. 31904249
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32520.1

Sequence Viewer

Length: 1569 bp
ATGAGAAATATTGTGTACTTTTTGAACTTGTTTTCCAAGTCACCGATTTGTTTTCGCAAAACTTTATCTGCAAGCTTCCCTATAAACCTCCCAGTTCTTGCTCTCTCTGTAACAATGGCGACTTCATGTTACTCTGCTTCCGACCCTCCCAATCCAGAGACTCCTTGCGTGCCTCCTTCTTACTCTCTTCGACGACAGCAAGAAGCCGACCTCTGCCATTATTTCGGCAACGTTCGGGTAACAGTGGACCAAAGCAGCACTAGTTCTCCGGGTTCGTCTTCATATGCTACTCCCACCAAAATCCAACAAGCGCAATCTGATTTTTATTCAAGACACGAAAGCGGGTTCAGAAATTGTCCGAGAGCTCCAATGGCATTGTATTCACAGAGTATATGGGGAGGCAATAGTGTGAGCGCTGGAGGAAACTTTATGGGCTCAAACAATCTGAATGTCCATCAACTAATTGGCAATCCGGCGACAGAACAAGGGACAAGAGCAAATTGGAGTGTTGGAGCAACGTCTACATCTTTGGGCAGTTGGGCACACCAGCTGAGCCCTTTTGGTAATAGCGATTGGAACCAAAATTATGGAGGACAAAATTACAGAGGCAATGTTGAAAGGACTGGATTATATTATTTCAATGATAATAATTGGGCCCAAAGTTCAGGAATACCTAGTGAGATAGCTCGACTCGCTATGACTCAACTCGGATCGCAGGCCTTGGTACAGAGAATGATAACTCCCGGCGACCCATTAGCAAAGAGTATGGTACTCGAAGGGGTTTTTGGTTCCTTATTCGAGGTGATGTGTGACTTTCATGGACACTATGTTTTTCGGAAGCTTGTTGAATCATGCAATCACAGTCGGTTGAGACTCATTGTGGCTAAGATAACTTTGAATTCTGAACCACTCATCAACGTATCAGTCTGCAATTATGGGTATCCATCTAATCCTTATTCCTTAACCCTCAAGAAAACAGTTTGCTTTGGAGAAATATATCTGCGTTTTATGCTTCATAGTTGTGAAGGACTGAAAATATATGAAGCTGTTGTGAGGCTTTGTGTCCGACTGGCTCAGCACGAAAGGGGATGCATACACTTGAATGAGTTCATCACCAACAGTAAAACTCCATACAGAGAGAAACTCATGGACAAAGTCTCGAGCAACTCAAAATTCCTTTCTCAGGATCCTTCAGGGAACTATGTTGTCCAGCACATCTTCACGCTATATAACCCAGTCTTCTCTGCAAAGATATGCTTTGAGCTTCGAAATCTTTACATACAACTCTCATTGCAAAAAGGCGGGAGTCACGTTGTAGAGATGTGCTTAAACTCTTTGGGAATGGAAGTTGTAGTGAATGTTCTTCTAAAGTATGACAAACTCTGGCAAGTTGCGCGAGATCAGTATGGAAATTACGTGATCCAAACAGCATTGAAGGCCACAAAGCGCAAAAATAGCCCCCTTCATCAAATGCTTCTAGAAAAGCTCAACAAAGATCGAAGTGAACTCATGGTTGGGTTTGGAAGGAAAGTGCTGAGCATCATTGACAAAGGGATCCCCCTAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

523

Amino Acids

58.39

Weight (kDa)

9.09

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01410 AT1G21620 AT1G35850 AT1G35850 AT5G59280 AT5G60110 AT5G60180 AT5G64490
fragaria_vesca FvH4_3g00680
malus_domestica MD05G1360200.v1.1
prunus_persica Prupe.4G007700_v2.0.a1
pyrus_communis pycom05g32520
rosa_chinensis RchiOBHm_Chr5g0000911
rosa_laevigata RLG00000030906
rosa_roxburghii Rroxscaffold_1G00075340
rosa_rugosa Rorug04G0387800 Rorug04G0387900.1
rosa_samantha Rh5AG007400 Rh5BG009500 Rh5CG008100 Rh5DG008100
rosa_wichuraiana Rw5G000770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 521
AccII CGCG 1 cut(s) 1396
AciI CCGC 2 cut(s) 342, 1302
AclI AACGTT 1 cut(s) 231
AclWI GGATC 6 cut(s) 718, 1181, 1194, 1414, 1549, 1562
AcsI RAATTY 2 cut(s) 898, 1172
AcuI CTGAAG 1 cut(s) 1176
AfaI GTAC 3 cut(s) 17, 726, 771
AfeI AGCGCT 1 cut(s) 415
AfiI CCNNNNNNNGG 1 cut(s) 1183
AgsI TTSAA 8 cut(s) 25, 330, 617, 640, 848, 898, 1102, 1435
AhlI ACTAGT 1 cut(s) 260
AjuI GAANNNNNNNTTGG 4 cut(s) 768, 800, 1497, 1529
AloI GAACNNNNNNTCC 2 cut(s) 17, 49
AluBI AGCT 8 cut(s) 75, 365, 550, 686, 841, 1046, 1264, 1486
AluI AGCT 8 cut(s) 75, 365, 550, 686, 841, 1046, 1264, 1486
Alw21I GWGCWC 1 cut(s) 367
Alw26I GTCTC 3 cut(s) 152, 865, 1162
AlwI GGATC 6 cut(s) 718, 1181, 1194, 1414, 1549, 1562
Ama87I CYCGRG 1 cut(s) 1159
Aor51HI AGCGCT 1 cut(s) 415
AoxI GGCC 3 cut(s) 654, 717, 1437
ApaI GGGCCC 1 cut(s) 658
ApeKI GCWGC 1 cut(s) 255
ApoI RAATTY 2 cut(s) 898, 1172
Asp700I GAANNNNTTC 1 cut(s) 1106
AspLEI GCGC 4 cut(s) 313, 416, 1396, 1449
AspS9I GGNCC 3 cut(s) 247, 654, 655
AsuC2I CCSGG 2 cut(s) 270, 744
AsuHPI GGTGA 3 cut(s) 33, 814, 1105
AsuII TTCGAA 1 cut(s) 1267
AvaI CYCGRG 1 cut(s) 1159
AvaII GGWCC 1 cut(s) 247
BaeGI GKGCMC 2 cut(s) 544, 658
BamHI GGATCC 2 cut(s) 1186, 1554
BanII GRGCYC 4 cut(s) 367, 437, 557, 658
BbsI GAAGAC 2 cut(s) 270, 1231
Bbv12I GWGCWC 1 cut(s) 367
BbvI GCAGC 1 cut(s) 267
BccI CCATC 2 cut(s) 462, 952
BciVI GTATCC 1 cut(s) 951
BcnI CCSGG 2 cut(s) 270, 744
BcoDI GTCTC 3 cut(s) 152, 865, 1162
BcuI ACTAGT 1 cut(s) 260
BfaI CTAG 4 cut(s) 261, 675, 1478, 1562
BfoI RGCGCY 1 cut(s) 417
BfuI GTATCC 1 cut(s) 951
BisI GCNGC 1 cut(s) 256
BlpI GCTNAGC 3 cut(s) 551, 1074, 1535
BlsI GCNGC 1 cut(s) 257
Bme1390I CCNGG 2 cut(s) 270, 744
Bme18I GGWCC 1 cut(s) 247
BmeT110I CYCGRG 1 cut(s) 1159
BmgT120I GGNCC 3 cut(s) 247, 654, 655
BmiI GGNNCC 5 cut(s) 578, 656, 790, 1188, 1556
BmrFI CCNGG 2 cut(s) 270, 744
BmrI ACTGGG 2 cut(s) 86, 1229
BmsI GCATC 2 cut(s) 1079, 1548
BmuI ACTGGG 2 cut(s) 86, 1229
BpiI GAAGAC 2 cut(s) 270, 1231
BplI GAGNNNNNCTC 2 cut(s) 1128, 1160
BpmI CTGGAG 1 cut(s) 438
Bpu1102I GCTNAGC 3 cut(s) 551, 1074, 1535
Bpu14I TTCGAA 1 cut(s) 1267
BpuEI CTTGAG 1 cut(s) 953
BpuMI CCSGG 2 cut(s) 270, 744
BsaAI YACGTR 1 cut(s) 1417
BsaJI CCNNGG 1 cut(s) 720
BsaXI ACNNNNNCTCC 2 cut(s) 250, 280
Bsc4I CCNNNNNNNGG 1 cut(s) 1183
Bse1I ACTGG 4 cut(s) 92, 628, 1074, 1235
Bse3DI GCAATG 2 cut(s) 616, 1289
BseDI CCNNGG 1 cut(s) 720
BseGI GGATG 1 cut(s) 1094
BseLI CCNNNNNNNGG 1 cut(s) 1183
BseMI GCAATG 2 cut(s) 616, 1289
BseMII CTCAG 4 cut(s) 542, 1088, 1196, 1526
BseNI ACTGG 4 cut(s) 92, 628, 1074, 1235
BseSI GKGCMC 2 cut(s) 544, 658
BseXI GCAGC 1 cut(s) 267
Bsh1236I CGCG 1 cut(s) 1396
BshFI GGCC 3 cut(s) 656, 719, 1439
BsiHKAI GWGCWC 1 cut(s) 367
BsiHKCI CYCGRG 1 cut(s) 1159
BsiSI CCGG 3 cut(s) 269, 473, 744
BslFI GGGAC 1 cut(s) 502
BslI CCNNNNNNNGG 1 cut(s) 1183
BsmAI GTCTC 3 cut(s) 152, 865, 1162
BsmFI GGGAC 1 cut(s) 502
BsnI GGCC 3 cut(s) 656, 719, 1439
BsoBI CYCGRG 1 cut(s) 1159
Bsp119I TTCGAA 1 cut(s) 1267
Bsp120I GGGCCC 1 cut(s) 654
Bsp1286I GDGCHC 5 cut(s) 367, 437, 544, 557, 658
Bsp143I GATC 6 cut(s) 710, 1186, 1399, 1419, 1495, 1554
Bsp1720I GCTNAGC 3 cut(s) 551, 1074, 1535
BspACI CCGC 2 cut(s) 342, 1302
BspANI GGCC 3 cut(s) 656, 719, 1439
BspCNI CTCAG 4 cut(s) 543, 1087, 1195, 1527
BspFNI CGCG 1 cut(s) 1396
BspLI GGNNCC 5 cut(s) 578, 656, 790, 1188, 1556
BspPI GGATC 6 cut(s) 718, 1181, 1194, 1414, 1549, 1562
BspT104I TTCGAA 1 cut(s) 1267
BsrDI GCAATG 2 cut(s) 616, 1289
BsrI ACTGG 4 cut(s) 92, 628, 1074, 1235
BssECI CCNNGG 1 cut(s) 720
BssMI GATC 6 cut(s) 710, 1186, 1399, 1419, 1495, 1554
BssT1I CCWWGG 1 cut(s) 720
Bst4CI ACNGT 4 cut(s) 244, 863, 979, 1121
Bst6I CTCTTC 1 cut(s) 192
BstBAI YACGTR 1 cut(s) 1417
BstBI TTCGAA 1 cut(s) 1267
BstC8I GCNNGC 3 cut(s) 73, 170, 717
BstDEI CTNAG 5 cut(s) 551, 885, 1074, 1182, 1535
BstENI CCTNNNNNAGG 1 cut(s) 1181
BstF5I GGATG 1 cut(s) 1094
BstFNI CGCG 1 cut(s) 1396
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 4 cut(s) 313, 416, 1396, 1449
BstKTI GATC 6 cut(s) 713, 1189, 1402, 1422, 1498, 1557
BstMAI GTCTC 3 cut(s) 152, 865, 1162
BstMBI GATC 6 cut(s) 710, 1186, 1399, 1419, 1495, 1554
BstMWI GCNNNNNNNGC 6 cut(s) 371, 692, 1009, 1393, 1436, 1455
BstSCI CCNGG 2 cut(s) 268, 742
BstSLI GKGCMC 2 cut(s) 544, 658
BstUI CGCG 1 cut(s) 1396
BstV1I GCAGC 1 cut(s) 267
BstV2I GAAGAC 2 cut(s) 270, 1231
BstX2I RGATCY 2 cut(s) 1186, 1554
BstXI CCANNNNNNTGG 1 cut(s) 587
BstYI RGATCY 2 cut(s) 1186, 1554
BsuI GTATCC 1 cut(s) 951
BsuRI GGCC 3 cut(s) 656, 719, 1439
BtsCI GGATG 1 cut(s) 1094
BtsIMutI CAGTG 1 cut(s) 249
Cac8I GCNNGC 3 cut(s) 73, 170, 717
CfoI GCGC 4 cut(s) 313, 416, 1396, 1449
Cfr13I GGNCC 3 cut(s) 247, 654, 655
Csp6I GTAC 3 cut(s) 16, 725, 770
CviAII CATG 5 cut(s) 126, 818, 852, 1147, 1510
CviQI GTAC 3 cut(s) 16, 725, 770
DdeI CTNAG 5 cut(s) 551, 885, 1074, 1182, 1535
DpnI GATC 6 cut(s) 712, 1188, 1401, 1421, 1497, 1556
DpnII GATC 6 cut(s) 710, 1186, 1399, 1419, 1495, 1554
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
Ecl136II GAGCTC 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 720
Eco147I AGGCCT 1 cut(s) 719
Eco24I GRGCYC 4 cut(s) 367, 437, 557, 658
Eco47I GGWCC 1 cut(s) 247
Eco47III AGCGCT 1 cut(s) 415
Eco53kI GAGCTC 1 cut(s) 365
Eco57I CTGAAG 1 cut(s) 1176
Eco88I CYCGRG 1 cut(s) 1159
EcoICRI GAGCTC 1 cut(s) 365
EcoNI CCTNNNNNAGG 1 cut(s) 1181
EcoRI GAATTC 1 cut(s) 898
EcoT14I CCWWGG 1 cut(s) 720
EcoT22I ATGCAT 1 cut(s) 1094
EcoT38I GRGCYC 4 cut(s) 367, 437, 557, 658
ErhI CCWWGG 1 cut(s) 720
FaeI CATG 5 cut(s) 129, 821, 855, 1150, 1513
FalI AAGNNNNNCTT 2 cut(s) 1241, 1273
FaqI GGGAC 1 cut(s) 502
FatI CATG 5 cut(s) 125, 817, 851, 1146, 1509
FauI CCCGC 2 cut(s) 335, 1295
FauNDI CATATG 1 cut(s) 283
FblI GTMKAC 1 cut(s) 521
Fnu4HI GCNGC 1 cut(s) 256
FokI GGATG 1 cut(s) 1101
FriOI GRGCYC 4 cut(s) 367, 437, 557, 658
Fsp4HI GCNGC 1 cut(s) 256
FspBI CTAG 4 cut(s) 261, 675, 1478, 1562
GlaI GCGC 4 cut(s) 312, 415, 1395, 1448
GluI GCNGC 1 cut(s) 256
GsuI CTGGAG 1 cut(s) 438
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 3 cut(s) 656, 719, 1439
HapII CCGG 3 cut(s) 269, 473, 744
HhaI GCGC 4 cut(s) 313, 416, 1396, 1449
Hin1II CATG 5 cut(s) 129, 821, 855, 1150, 1513
Hin6I GCGC 4 cut(s) 311, 414, 1394, 1447
HinP1I GCGC 4 cut(s) 311, 414, 1394, 1447
HindIII AAGCTT 2 cut(s) 73, 839
HinfI GANTC 6 cut(s) 160, 690, 700, 848, 873, 1306
HpaII CCGG 3 cut(s) 269, 473, 744
HphI GGTGA 3 cut(s) 33, 814, 1105
Hpy166II GTNNAC 4 cut(s) 16, 247, 522, 1505
Hpy188I TCNGA 9 cut(s) 142, 319, 350, 360, 447, 710, 837, 904, 1067
Hpy188III TCNNGA 7 cut(s) 155, 330, 666, 970, 1159, 1184, 1478
Hpy8I GTNNAC 4 cut(s) 16, 247, 522, 1505
Hpy99I CGWCG 1 cut(s) 195
HpyAV CCTTC 7 cut(s) 186, 770, 1019, 1200, 1429, 1472, 1518
HpyCH4III ACNGT 4 cut(s) 244, 863, 979, 1121
HpyCH4IV ACGT 5 cut(s) 231, 518, 918, 1311, 1416
HpyCH4V TGCA 6 cut(s) 71, 855, 930, 1092, 1247, 1294
HpyF10VI GCNNNNNNNGC 6 cut(s) 371, 692, 1009, 1393, 1436, 1455
HpyF3I CTNAG 5 cut(s) 551, 885, 1074, 1182, 1535
HpySE526I ACGT 5 cut(s) 231, 518, 918, 1311, 1416
Hsp92II CATG 5 cut(s) 129, 821, 855, 1150, 1513
HspAI GCGC 4 cut(s) 311, 414, 1394, 1447
Kzo9I GATC 6 cut(s) 710, 1186, 1399, 1419, 1495, 1554
LmnI GCTCC 2 cut(s) 370, 512
Lsp1109I GCAGC 1 cut(s) 267
LweI GCATC 2 cut(s) 1079, 1548
MaeI CTAG 4 cut(s) 261, 675, 1478, 1562
MaeII ACGT 5 cut(s) 231, 518, 918, 1311, 1416
MaeIII GTNAC 6 cut(s) 39, 109, 128, 238, 809, 1307
MalI GATC 6 cut(s) 712, 1188, 1401, 1421, 1497, 1556
MboI GATC 6 cut(s) 710, 1186, 1399, 1419, 1495, 1554
MboII GAAGA 5 cut(s) 179, 270, 1210, 1231, 1355
MflI RGATCY 2 cut(s) 1186, 1554
MhlI GDGCHC 5 cut(s) 367, 437, 544, 557, 658
MlyI GAGTC 5 cut(s) 154, 684, 694, 867, 1315
MmeI TCCRAC 4 cut(s) 165, 328, 490, 1090
Mph1103I ATGCAT 1 cut(s) 1094
MroXI GAANNNNTTC 1 cut(s) 1106
MseI TTAA 2 cut(s) 962, 1328
MslI CAYNNNNRTG 2 cut(s) 1020, 1101
MspA1I CMGCKG 1 cut(s) 550
MspI CCGG 3 cut(s) 269, 473, 744
MspR9I CCNGG 2 cut(s) 270, 744
MvnI CGCG 1 cut(s) 1396
MwoI GCNNNNNNNGC 6 cut(s) 371, 692, 1009, 1393, 1436, 1455
NciI CCSGG 2 cut(s) 270, 744
NdeI CATATG 1 cut(s) 283
NdeII GATC 6 cut(s) 710, 1186, 1399, 1419, 1495, 1554
NlaIII CATG 5 cut(s) 129, 821, 855, 1150, 1513
NlaIV GGNNCC 5 cut(s) 578, 656, 790, 1188, 1556
NmuCI GTSAC 3 cut(s) 39, 809, 1307
NsiI ATGCAT 1 cut(s) 1094
NspV TTCGAA 1 cut(s) 1267
PaeR7I CTCGAG 1 cut(s) 1159
PceI AGGCCT 1 cut(s) 719
PdmI GAANNNNTTC 1 cut(s) 1106
PfeI GAWTC 1 cut(s) 848
PflFI GACNNNGTC 1 cut(s) 1154
PkrI GCNGC 1 cut(s) 257
PleI GAGTC 5 cut(s) 154, 684, 694, 867, 1314
PpsI GAGTC 5 cut(s) 154, 684, 694, 867, 1314
Ppu21I YACGTR 1 cut(s) 1417
Psp124BI GAGCTC 1 cut(s) 367
Psp1406I AACGTT 1 cut(s) 231
PspN4I GGNNCC 5 cut(s) 578, 656, 790, 1188, 1556
PspOMI GGGCCC 1 cut(s) 654
PspPI GGNCC 3 cut(s) 247, 654, 655
PsrI GAACNNNNNNTAC 2 cut(s) 1344, 1376
PsuI RGATCY 2 cut(s) 1186, 1554
PsyI GACNNNGTC 1 cut(s) 1154
PvuII CAGCTG 1 cut(s) 550
RsaI GTAC 3 cut(s) 17, 726, 771
RsaNI GTAC 3 cut(s) 16, 725, 770
RseI CAYNNNNRTG 2 cut(s) 1020, 1101
SacI GAGCTC 1 cut(s) 367
SaqAI TTAA 2 cut(s) 962, 1328
SatI GCNGC 1 cut(s) 256
Sau3AI GATC 6 cut(s) 710, 1186, 1399, 1419, 1495, 1554
Sau96I GGNCC 3 cut(s) 247, 654, 655
SchI GAGTC 5 cut(s) 154, 684, 694, 867, 1315
ScrFI CCNGG 2 cut(s) 270, 744
SduI GDGCHC 5 cut(s) 367, 437, 544, 557, 658
SfaNI GCATC 2 cut(s) 1079, 1548
Sfr274I CTCGAG 1 cut(s) 1159
SfuI TTCGAA 1 cut(s) 1267
SinI GGWCC 1 cut(s) 247
SlaI CTCGAG 1 cut(s) 1159
SmiMI CAYNNNNRTG 2 cut(s) 1020, 1101
SmlI CTYRAG 2 cut(s) 968, 1159
SmoI CTYRAG 2 cut(s) 968, 1159
SpeI ACTAGT 1 cut(s) 260
SseBI AGGCCT 1 cut(s) 719
SsiI CCGC 2 cut(s) 342, 1302
SspI AATATT 1 cut(s) 10
SspMI CTAG 4 cut(s) 261, 675, 1478, 1562
SstI GAGCTC 1 cut(s) 367
StuI AGGCCT 1 cut(s) 719
StyD4I CCNGG 2 cut(s) 268, 742
StyI CCWWGG 1 cut(s) 720
TaaI ACNGT 4 cut(s) 244, 863, 979, 1121
TaiI ACGT 5 cut(s) 234, 521, 921, 1314, 1419
TaqI TCGA 7 cut(s) 190, 688, 774, 798, 1160, 1267, 1498
TatI WGTACW 1 cut(s) 15
TfiI GAWTC 1 cut(s) 848
Tru1I TTAA 2 cut(s) 962, 1328
Tru9I TTAA 2 cut(s) 962, 1328
TscAI CASTG 1 cut(s) 249
TseFI GTSAC 3 cut(s) 39, 809, 1307
TseI GCWGC 1 cut(s) 255
Tsp45I GTSAC 3 cut(s) 39, 809, 1307
TspDTI ATGAA 7 cut(s) 114, 270, 806, 1004, 1056, 1099, 1454
TspRI CASTG 1 cut(s) 249
Tth111I GACNNNGTC 1 cut(s) 1154
VpaK11BI GGWCC 1 cut(s) 247
XagI CCTNNNNNAGG 1 cut(s) 1181
XapI RAATTY 2 cut(s) 898, 1172
XbaI TCTAGA 1 cut(s) 1477
XcmI CCANNNNNNNNNTGG 1 cut(s) 461
XhoI CTCGAG 1 cut(s) 1159
XmiI GTMKAC 1 cut(s) 521
XmnI GAANNNNTTC 1 cut(s) 1106
XspI CTAG 4 cut(s) 261, 675, 1478, 1562
Zsp2I ATGCAT 1 cut(s) 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.