AT5G60180

Arabidopsis Pumilio

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009111
Physical Location & Seq
Reverse (-)
24232875 .. 24234135
1261 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G60180.1

Sequence Viewer

Length: 984 bp
ATGGCAGTCTCCGATAATACCTTTTCAATGTCGACCATGTTCAATGCTTTACCAAACCCTACAGAAACCTCCGGTTACATTCCTCCTTCTGGATTCGCCCCACGCGCCTCCGCCACGCCCTTGCACGCAGCTCTGTTCAACCTAATGACTGACGGCGATGGTGTGTCTTACTTTAAAGAGATGATCTCAAAGTCAGACAAAACAGAGCTTCAGAGGATGGCCTCGTTGCTTACGTCTGACTCCGATTACTTCATGTCGATCGTCACAACCAAGTTCGGCTCGAGACGCGTGCAGAAGCTCCTCGGCAAATCAGATGACGTGGACGCCTTCTTCTGTGCCGCTATCTTGCGCCGCTTCCTCCACATCACGACCGACAAATACGCATCCTACGTGACGATACGAGCCATGGTCGTTTTCGACAAGGTGATGAAAAAGGCATTGTACGAGCGCATTCTCTACCACGCTCTCGACCTAGCGTGTGACCAGCACGGCTGCATCGCGCTCAATGATATCATAACCGACGCTGACGATCCTTACTACAGAGATCAGCTACTAGAATTGGTCGTATCCAACGCTCTCCGGCTAAGCAACGATGCTTCAGGGAACTTTGTGGTCCAACATGTGCTTACACTGTATGACTCGCGTTGCATTCACAACATCGCCGTTAATCTTTATGGTCAGTGCATTGAGCTATCGTTTAAGAAGTATGGAAGCTACATCGTGGAGAAGCTTTTGGAGGTGGAGGAGTCAATGGTTGTGGTGGTTGTGGAGCTTTTGGGTTGCGATGGAGACAGGTTGATGAGGCTGGCGAGGAATGAGTTTGGGAATTTCGTGGTGGTCAAGGCACTGAGATTCACAAAGGAGATGAGGATGGATCTGTTTTGGGGTTTGGTGCAGAAGCTCATGCCTTTTATCCATCTCTTGCGTAGATCTCACGGAAACAACATTGCAAACATCTTGGACACATTTAGGCTTCGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

327

Amino Acids

37.06

Weight (kDa)

6.71

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01410 AT1G21620 AT1G35850 AT1G35850 AT5G59280 AT5G60110 AT5G60180 AT5G64490
fragaria_vesca FvH4_3g00680
malus_domestica MD05G1360200.v1.1
prunus_persica Prupe.4G007700_v2.0.a1
pyrus_communis pycom05g32520
rosa_chinensis RchiOBHm_Chr5g0000911
rosa_laevigata RLG00000030906
rosa_roxburghii Rroxscaffold_1G00075340
rosa_rugosa Rorug04G0387800 Rorug04G0387900.1
rosa_samantha Rh5AG007400 Rh5BG009500 Rh5CG008100 Rh5DG008100
rosa_wichuraiana Rw5G000770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 32
AccII CGCG 4 cut(s) 105, 288, 500, 643
AciI CCGC 3 cut(s) 111, 339, 352
AclWI GGATC 2 cut(s) 524, 882
AcsI RAATTY 1 cut(s) 826
AcuI CTGAAG 2 cut(s) 194, 582
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 89
AflIII ACRYGT 2 cut(s) 286, 619
AgsI TTSAA 3 cut(s) 27, 43, 139
AjiI CACGTC 1 cut(s) 319
AluBI AGCT 9 cut(s) 131, 208, 298, 550, 691, 714, 730, 772, 901
AluI AGCT 9 cut(s) 131, 208, 298, 550, 691, 714, 730, 772, 901
Alw26I GTCTC 3 cut(s) 13, 277, 783
AlwI GGATC 2 cut(s) 524, 882
Ama87I CYCGRG 1 cut(s) 280
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 2 cut(s) 128, 492
ApoI RAATTY 1 cut(s) 826
AspLEI GCGC 4 cut(s) 107, 351, 450, 502
AspS9I GGNCC 1 cut(s) 613
AsuHPI GGTGA 1 cut(s) 436
AvaI CYCGRG 1 cut(s) 280
AvaII GGWCC 1 cut(s) 613
BbvI GCAGC 2 cut(s) 140, 479
BccI CCATC 5 cut(s) 152, 211, 779, 865, 924
BceAI ACGGC 3 cut(s) 169, 505, 647
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BciVI GTATCC 1 cut(s) 577
BcoDI GTCTC 3 cut(s) 13, 277, 783
BfaI CTAG 2 cut(s) 473, 554
BfmI CTRYAG 2 cut(s) 60, 538
BfuI GTATCC 1 cut(s) 577
BglII AGATCT 1 cut(s) 929
BisI GCNGC 4 cut(s) 129, 339, 352, 493
BlpI GCTNAGC 1 cut(s) 584
BlsI GCNGC 4 cut(s) 130, 340, 353, 494
Bme18I GGWCC 1 cut(s) 613
BmeT110I CYCGRG 1 cut(s) 280
BmgBI CACGTC 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 613
BmsI GCATC 3 cut(s) 392, 504, 583
BplI GAGNNNNNCTC 2 cut(s) 170, 202
Bpu1102I GCTNAGC 1 cut(s) 584
BsaAI YACGTR 1 cut(s) 391
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 301, 405
BsaWI WCCGGW 1 cut(s) 71
Bsc4I CCNNNNNNNGG 1 cut(s) 89
Bse3DI GCAATG 1 cut(s) 945
BseDI CCNNGG 2 cut(s) 301, 405
BseGI GGATG 3 cut(s) 222, 383, 876
BseLI CCNNNNNNNGG 1 cut(s) 89
BseMI GCAATG 1 cut(s) 945
BseMII CTCAG 1 cut(s) 839
BseRI GAGGAG 2 cut(s) 290, 758
BseXI GCAGC 2 cut(s) 140, 479
BsgI GTGCAG 2 cut(s) 311, 914
Bsh1236I CGCG 4 cut(s) 105, 288, 500, 643
Bsh1285I CGRYCG 2 cut(s) 261, 372
BshFI GGCC 1 cut(s) 221
BsiEI CGRYCG 2 cut(s) 261, 372
BsiHKCI CYCGRG 1 cut(s) 280
BsiSI CCGG 2 cut(s) 72, 580
BslI CCNNNNNNNGG 1 cut(s) 89
BsmAI GTCTC 3 cut(s) 13, 277, 783
BsmBI CGTCTC 1 cut(s) 277
BsmI GAATGC 2 cut(s) 450, 648
BsnI GGCC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 280
Bsp143I GATC 6 cut(s) 183, 258, 529, 544, 874, 929
Bsp1720I GCTNAGC 1 cut(s) 584
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 3 cut(s) 111, 339, 352
BspANI GGCC 1 cut(s) 221
BspCNI CTCAG 1 cut(s) 840
BspFNI CGCG 4 cut(s) 105, 288, 500, 643
BspPI GGATC 2 cut(s) 524, 882
BsrDI GCAATG 1 cut(s) 945
BssECI CCNNGG 2 cut(s) 301, 405
BssMI GATC 6 cut(s) 183, 258, 529, 544, 874, 929
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 1 cut(s) 405
Bst4CI ACNGT 1 cut(s) 633
BstACI GRCGYC 1 cut(s) 324
BstBAI YACGTR 1 cut(s) 391
BstC8I GCNNGC 3 cut(s) 126, 290, 807
BstDEI CTNAG 2 cut(s) 584, 848
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 3 cut(s) 222, 383, 876
BstFNI CGCG 4 cut(s) 105, 288, 500, 643
BstHHI GCGC 4 cut(s) 107, 351, 450, 502
BstKTI GATC 6 cut(s) 186, 261, 532, 547, 877, 932
BstMAI GTCTC 3 cut(s) 13, 277, 783
BstMBI GATC 6 cut(s) 183, 258, 529, 544, 874, 929
BstMCI CGRYCG 2 cut(s) 261, 372
BstMWI GCNNNNNNNGC 2 cut(s) 104, 285
BstNSI RCATGY 1 cut(s) 623
BstSFI CTRYAG 2 cut(s) 60, 538
BstUI CGCG 4 cut(s) 105, 288, 500, 643
BstV1I GCAGC 2 cut(s) 140, 479
BstX2I RGATCY 2 cut(s) 874, 929
BstYI RGATCY 2 cut(s) 874, 929
BsuI GTATCC 1 cut(s) 577
BsuRI GGCC 1 cut(s) 221
BtgI CCRYGG 1 cut(s) 405
BtgZI GCGATG 4 cut(s) 171, 481, 643, 798
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 3 cut(s) 222, 383, 876
BtsIMutI CAGTG 3 cut(s) 629, 686, 845
Cac8I GCNNGC 3 cut(s) 126, 290, 807
CfoI GCGC 4 cut(s) 107, 351, 450, 502
Cfr13I GGNCC 1 cut(s) 613
CseI GACGC 3 cut(s) 294, 332, 530
Csp6I GTAC 1 cut(s) 442
CspCI CAANNNNNGTGG 2 cut(s) 738, 773
CviAII CATG 5 cut(s) 37, 253, 406, 620, 904
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 2 cut(s) 584, 848
DpnI GATC 6 cut(s) 185, 260, 531, 546, 876, 931
DpnII GATC 6 cut(s) 183, 258, 529, 544, 874, 929
DraI TTTAAA 1 cut(s) 175
EciI GGCGGA 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 405
Eco32I GATATC 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 613
Eco57I CTGAAG 2 cut(s) 194, 582
Eco88I CYCGRG 1 cut(s) 280
EcoRV GATATC 1 cut(s) 511
EcoT14I CCWWGG 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 405
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 5 cut(s) 40, 256, 409, 623, 907
FaiI YATR 9 cut(s) 38, 254, 407, 515, 621, 636, 675, 708, 905
FatI CATG 5 cut(s) 36, 252, 405, 619, 903
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 4 cut(s) 129, 339, 352, 493
FokI GGATG 3 cut(s) 229, 370, 883
Fsp4HI GCNGC 4 cut(s) 129, 339, 352, 493
FspBI CTAG 2 cut(s) 473, 554
GlaI GCGC 4 cut(s) 106, 350, 449, 501
GluI GCNGC 4 cut(s) 129, 339, 352, 493
HaeIII GGCC 1 cut(s) 221
HapII CCGG 2 cut(s) 72, 580
HgaI GACGC 3 cut(s) 294, 332, 530
HhaI GCGC 4 cut(s) 107, 351, 450, 502
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 5 cut(s) 40, 256, 409, 623, 907
Hin6I GCGC 4 cut(s) 105, 349, 448, 500
HinP1I GCGC 4 cut(s) 105, 349, 448, 500
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 5 cut(s) 93, 239, 638, 746, 852
HpaII CCGG 2 cut(s) 72, 580
HphI GGTGA 1 cut(s) 436
Hpy166II GTNNAC 2 cut(s) 33, 322
Hpy188I TCNGA 6 cut(s) 13, 196, 213, 238, 244, 313
Hpy188III TCNNGA 4 cut(s) 90, 282, 367, 467
Hpy8I GTNNAC 2 cut(s) 33, 322
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 2 cut(s) 96, 337
HpyCH4III ACNGT 1 cut(s) 633
HpyCH4IV ACGT 3 cut(s) 233, 318, 390
HpyCH4V TGCA 7 cut(s) 124, 292, 495, 648, 684, 895, 950
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 285
HpyF3I CTNAG 2 cut(s) 584, 848
HpySE526I ACGT 3 cut(s) 233, 318, 390
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 5 cut(s) 40, 256, 409, 623, 907
HspAI GCGC 4 cut(s) 105, 349, 448, 500
Kzo9I GATC 6 cut(s) 183, 258, 529, 544, 874, 929
LmnI GCTCC 2 cut(s) 303, 769
LpnPI CCDG 7 cut(s) 75, 85, 497, 585, 593, 778, 791
Lsp1109I GCAGC 2 cut(s) 140, 479
LweI GCATC 3 cut(s) 392, 504, 583
MaeI CTAG 2 cut(s) 473, 554
MaeII ACGT 3 cut(s) 233, 318, 390
MaeIII GTNAC 4 cut(s) 74, 262, 391, 479
MalI GATC 6 cut(s) 185, 260, 531, 546, 876, 931
MboI GATC 6 cut(s) 183, 258, 529, 544, 874, 929
MboII GAAGA 1 cut(s) 322
MflI RGATCY 2 cut(s) 874, 929
MluCI AATT 2 cut(s) 557, 826
MluI ACGCGT 1 cut(s) 286
MlyI GAGTC 3 cut(s) 233, 632, 755
MmeI TCCRAC 2 cut(s) 594, 640
MseI TTAA 3 cut(s) 174, 666, 699
MspI CCGG 2 cut(s) 72, 580
Mva1269I GAATGC 2 cut(s) 450, 648
MvnI CGCG 4 cut(s) 105, 288, 500, 643
MwoI GCNNNNNNNGC 2 cut(s) 104, 285
NcoI CCATGG 1 cut(s) 405
NdeII GATC 6 cut(s) 183, 258, 529, 544, 874, 929
NlaIII CATG 5 cut(s) 40, 256, 409, 623, 907
NmeAIII GCCGAG 1 cut(s) 282
NmuCI GTSAC 3 cut(s) 262, 391, 479
NspI RCATGY 1 cut(s) 623
PaeR7I CTCGAG 1 cut(s) 280
PciI ACATGT 1 cut(s) 619
PcsI WCGNNNNNNNCGW 2 cut(s) 230, 387
PctI GAATGC 2 cut(s) 450, 648
PfeI GAWTC 2 cut(s) 93, 852
PkrI GCNGC 4 cut(s) 130, 340, 353, 494
Ple19I CGATCG 1 cut(s) 261
PleI GAGTC 3 cut(s) 233, 632, 754
PpsI GAGTC 3 cut(s) 233, 632, 754
Ppu21I YACGTR 1 cut(s) 391
PscI ACATGT 1 cut(s) 619
PspPI GGNCC 1 cut(s) 613
PsuI RGATCY 2 cut(s) 874, 929
PvuI CGATCG 1 cut(s) 261
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
SalI GTCGAC 1 cut(s) 31
SaqAI TTAA 3 cut(s) 174, 666, 699
SatI GCNGC 4 cut(s) 129, 339, 352, 493
Sau3AI GATC 6 cut(s) 183, 258, 529, 544, 874, 929
Sau96I GGNCC 1 cut(s) 613
SchI GAGTC 3 cut(s) 233, 632, 755
SfaNI GCATC 3 cut(s) 392, 504, 583
SfcI CTRYAG 2 cut(s) 60, 538
Sfr274I CTCGAG 1 cut(s) 280
SinI GGWCC 1 cut(s) 613
SlaI CTCGAG 1 cut(s) 280
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 2 cut(s) 557, 826
SsiI CCGC 3 cut(s) 111, 339, 352
SspMI CTAG 2 cut(s) 473, 554
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 1 cut(s) 633
TaiI ACGT 3 cut(s) 236, 321, 393
TaqI TCGA 5 cut(s) 32, 257, 281, 417, 468
TaqII GACCGA 1 cut(s) 386
TasI AATT 2 cut(s) 557, 826
TauI GCSGC 2 cut(s) 341, 354
TfiI GAWTC 2 cut(s) 93, 852
Tru1I TTAA 3 cut(s) 174, 666, 699
Tru9I TTAA 3 cut(s) 174, 666, 699
TscAI CASTG 3 cut(s) 636, 686, 852
TseFI GTSAC 3 cut(s) 262, 391, 479
TseI GCWGC 2 cut(s) 128, 492
Tsp45I GTSAC 3 cut(s) 262, 391, 479
TspDTI ATGAA 2 cut(s) 241, 443
TspGWI ACGGA 1 cut(s) 951
TspRI CASTG 3 cut(s) 636, 686, 852
VpaK11BI GGWCC 1 cut(s) 613
XapI RAATTY 1 cut(s) 826
XceI RCATGY 1 cut(s) 623
XhoI CTCGAG 1 cut(s) 280
XmiI GTMKAC 1 cut(s) 32
XspI CTAG 2 cut(s) 473, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.