Rroxscaffold_1G00075340

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009111
Physical Location & Seq
Reverse (-)
96107160 .. 96109520
2361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075340.1

Sequence Viewer

Length: 1299 bp
ATGCCCCAACAACAATCAGAGGCTTATTGTTATCCCAAACATGAAATCGGGTTCGAAAATTCTAACTCTGTTCCAATTCCTTTATATTCACAAGATATTTGGAGAGGTCAAAGTGACAACGCTGCAGGACAACTTTTGGGTTCAAACAACCTGAATGGGCAAGTTTTGACAAGGAATGGGAACCCTAGACTAGATGCATATGCTGGTTTTCAATCTCATGAAGTGGCTGAGAAAAGTGGAATGTATTCAAATAATAATTGGTATCGAAGTTCGGGAATATTGAATGACATGGGGAACCAATCAAGCTTTGGCAATGACTCGATGCTGGGAAGTAGCTATATAGCTCAAACGGCTATGACTCAATATGGCTCACAGTACTTGCTAGATAGCTTGTTGACTAAGGACCCCGTAGTTACAAATTTTATATTTGAAGGTGTTTTCAATTTCCTATTCGAGGTGATGAATGATTCAAATGGACATCATCTTTTTGGGAAGCTAGTTCAGTCATGCAGCGATAATCAGTTAGGCCTCATTGTAGCAAAGATAACTTTGAATGTCCAATTGCTTATCAACATATCAGTCGGCAGATATGGCTCAAAATCGGTTCAGAGGCTGATCAAGGTGCTTGAGAAATCACCCTTGATTGACATTGTCATAGCAGCCCTATCCTGTGGATTTGAAGAACTGATGACCAATCGCTCAGGATCCTTTGTTATATTGAAGTGCTTAAACCTCCTTGATACTGAGAAAAATCAGAAACTATATGAAGCTGCTGTAAATCTTTGCATCCCATTGGCTCAGAATGAAAAGGGATGTATATACTTGAATGAGTTCATCACCAACAGTAAAGCTCCATATAGAGAGACACTCATGGGTGAGATCTCAAGCAAATCAAAATTCCTTTCTCAGGATCCTTCAGGGAACTTTGTTGTTCAGCACATCCTCGGACTCCACAATCCCATTTACGGTGAGCGCATATGTTTGGAGCTGAGAGAACTTTACATACAGCTCTCGCAACAAAAAGGTGGCAGTCACGTTGTGGAGAAGTGCTTAAACTCATCCGGGATGGTTCATGTGGTAAGTGTTCTTCTCAAGTCTGAAAAACTTTGGCAAGTTGCGAGGGATCAATATGGAAACTACGTGATCCAAACAGCATTGAAGACCACAAAACGCGCGAATAGTCCCCTTCATCAAATGCTTATATCGAAGCTCGTACAGAATCGGAATGAACTTGTGATTGGATTTGGAAGGAAAGTGCTTGGTTTGATTGACAATGGGATCCCACTAGACCAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

432

Amino Acids

48.05

Weight (kDa)

7.1

Isoelectric Point (pI)

29.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01410 AT1G21620 AT1G35850 AT1G35850 AT5G59280 AT5G60110 AT5G60180 AT5G64490
fragaria_vesca FvH4_3g00680
malus_domestica MD05G1360200.v1.1
prunus_persica Prupe.4G007700_v2.0.a1
pyrus_communis pycom05g32520
rosa_chinensis RchiOBHm_Chr5g0000911
rosa_laevigata RLG00000030906
rosa_roxburghii Rroxscaffold_1G00075340
rosa_rugosa Rorug04G0387800 Rorug04G0387900.1
rosa_samantha Rh5AG007400 Rh5BG009500 Rh5CG008100 Rh5DG008100
rosa_wichuraiana Rw5G000770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 1173, 1175
AclWI GGATC 8 cut(s) 699, 712, 905, 918, 1131, 1138, 1273, 1286
AcsI RAATTY 3 cut(s) 58, 418, 896
AcuI CTGAAG 1 cut(s) 900
AdeI CACNNNGTG 1 cut(s) 1039
AfaI GTAC 2 cut(s) 377, 1215
AfiI CCNNNNNNNGG 3 cut(s) 454, 907, 965
AjuI GAANNNNNNNTTGG 2 cut(s) 1221, 1253
Alw26I GTCTC 1 cut(s) 857
AlwI GGATC 8 cut(s) 699, 712, 905, 918, 1131, 1138, 1273, 1286
AlwNI CAGNNNCTG 1 cut(s) 613
AoxI GGCC 1 cut(s) 526
ApeKI GCWGC 4 cut(s) 122, 510, 659, 770
ApoI RAATTY 3 cut(s) 58, 418, 896
Asp700I GAANNNNTTC 2 cut(s) 244, 830
AspLEI GCGC 2 cut(s) 975, 1175
AspS9I GGNCC 1 cut(s) 403
AsuC2I CCSGG 1 cut(s) 1063
AsuHPI GGTGA 5 cut(s) 469, 627, 829, 887, 980
AsuII TTCGAA 1 cut(s) 54
AvaII GGWCC 1 cut(s) 403
BamHI GGATCC 3 cut(s) 704, 910, 1278
BarI GAAGNNNNNNTAC 2 cut(s) 1071, 1103
BbsI GAAGAC 1 cut(s) 1166
BbvI GCAGC 4 cut(s) 109, 522, 671, 757
BccI CCATC 1 cut(s) 1060
BceAI ACGGC 1 cut(s) 366
BclI TGATCA 1 cut(s) 615
BcnI CCSGG 1 cut(s) 1063
BcoDI GTCTC 1 cut(s) 857
BfaI CTAG 5 cut(s) 186, 191, 383, 497, 1286
BfmI CTRYAG 1 cut(s) 123
BglII AGATCT 1 cut(s) 879
BisI GCNGC 4 cut(s) 123, 511, 660, 771
BlsI GCNGC 4 cut(s) 124, 512, 661, 772
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 1 cut(s) 1063
Bme18I GGWCC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 6 cut(s) 182, 296, 405, 706, 912, 1280
BmrFI CCNGG 1 cut(s) 1063
BmsI GCATC 3 cut(s) 184, 312, 795
BpiI GAAGAC 1 cut(s) 1166
BplI GAGNNNNNCTC 2 cut(s) 852, 884
Bpu10I CCTNAGC 1 cut(s) 700
Bpu14I TTCGAA 1 cut(s) 54
BpuEI CTTGAG 3 cut(s) 647, 868, 1076
BpuMI CCSGG 1 cut(s) 1063
BsaAI YACGTR 1 cut(s) 1141
BsaJI CCNNGG 1 cut(s) 943
Bsc4I CCNNNNNNNGG 3 cut(s) 454, 907, 965
Bse3DI GCAATG 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 943
BseGI GGATG 5 cut(s) 786, 818, 939, 1058, 1071
BseLI CCNNNNNNNGG 3 cut(s) 454, 907, 965
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 6 cut(s) 219, 714, 735, 812, 920, 980
BseXI GCAGC 4 cut(s) 109, 522, 671, 757
BseYI CCCAGC 1 cut(s) 325
Bsh1236I CGCG 2 cut(s) 1173, 1175
BshFI GGCC 1 cut(s) 528
BsiSI CCGG 1 cut(s) 1062
BslFI GGGAC 1 cut(s) 1167
BslI CCNNNNNNNGG 3 cut(s) 454, 907, 965
BsmAI GTCTC 1 cut(s) 857
BsmFI GGGAC 1 cut(s) 1167
BsnI GGCC 1 cut(s) 528
Bsp119I TTCGAA 1 cut(s) 54
Bsp143I GATC 7 cut(s) 615, 704, 879, 910, 1123, 1143, 1278
BspANI GGCC 1 cut(s) 528
BspCNI CTCAG 6 cut(s) 220, 713, 736, 811, 919, 981
BspFNI CGCG 2 cut(s) 1173, 1175
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 6 cut(s) 182, 296, 405, 706, 912, 1280
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 8 cut(s) 699, 712, 905, 918, 1131, 1138, 1273, 1286
BspT104I TTCGAA 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 943
BssMI GATC 7 cut(s) 615, 704, 879, 910, 1123, 1143, 1278
Bst4CI ACNGT 3 cut(s) 375, 845, 968
BstBAI YACGTR 1 cut(s) 1141
BstBI TTCGAA 1 cut(s) 54
BstDEI CTNAG 7 cut(s) 228, 399, 700, 744, 798, 906, 989
BstENI CCTNNNNNAGG 2 cut(s) 452, 905
BstF5I GGATG 5 cut(s) 786, 818, 939, 1058, 1071
BstFNI CGCG 2 cut(s) 1173, 1175
BstHHI GCGC 2 cut(s) 975, 1175
BstKTI GATC 7 cut(s) 618, 707, 882, 913, 1126, 1146, 1281
BstMAI GTCTC 1 cut(s) 857
BstMBI GATC 7 cut(s) 615, 704, 879, 910, 1123, 1143, 1278
BstMWI GCNNNNNNNGC 2 cut(s) 350, 591
BstSCI CCNGG 1 cut(s) 1061
BstSFI CTRYAG 1 cut(s) 123
BstUI CGCG 2 cut(s) 1173, 1175
BstV1I GCAGC 4 cut(s) 109, 522, 671, 757
BstV2I GAAGAC 1 cut(s) 1166
BstX2I RGATCY 4 cut(s) 704, 879, 910, 1278
BstYI RGATCY 4 cut(s) 704, 879, 910, 1278
BsuRI GGCC 1 cut(s) 528
BtsCI GGATG 5 cut(s) 786, 818, 939, 1058, 1071
CaiI CAGNNNCTG 1 cut(s) 613
CciI TCATGA 1 cut(s) 217
CfoI GCGC 2 cut(s) 975, 1175
Cfr13I GGNCC 1 cut(s) 403
Csp6I GTAC 2 cut(s) 376, 1214
CviAII CATG 6 cut(s) 41, 218, 289, 507, 871, 1073
CviQI GTAC 2 cut(s) 376, 1214
DdeI CTNAG 7 cut(s) 228, 399, 700, 744, 798, 906, 989
DpnI GATC 7 cut(s) 617, 706, 881, 912, 1125, 1145, 1280
DpnII GATC 7 cut(s) 615, 704, 879, 910, 1123, 1143, 1278
DraIII CACNNNGTG 1 cut(s) 1039
Eco147I AGGCCT 1 cut(s) 528
Eco47I GGWCC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 900
EcoNI CCTNNNNNAGG 2 cut(s) 452, 905
EcoO109I RGGNCCY 1 cut(s) 403
EcoT22I ATGCAT 1 cut(s) 199
FaeI CATG 6 cut(s) 44, 221, 292, 510, 874, 1076
FaqI GGGAC 1 cut(s) 1167
FatI CATG 6 cut(s) 40, 217, 288, 506, 870, 1072
FauNDI CATATG 2 cut(s) 199, 977
FbaI TGATCA 1 cut(s) 615
Fnu4HI GCNGC 4 cut(s) 123, 511, 660, 771
FokI GGATG 5 cut(s) 773, 825, 926, 1045, 1078
Fsp4HI GCNGC 4 cut(s) 123, 511, 660, 771
FspBI CTAG 5 cut(s) 186, 191, 383, 497, 1286
GlaI GCGC 2 cut(s) 974, 1174
GluI GCNGC 4 cut(s) 123, 511, 660, 771
GsaI CCCAGC 1 cut(s) 329
HaeIII GGCC 1 cut(s) 528
HapII CCGG 1 cut(s) 1062
HhaI GCGC 2 cut(s) 975, 1175
Hin1II CATG 6 cut(s) 44, 221, 292, 510, 874, 1076
Hin6I GCGC 2 cut(s) 973, 1173
HinP1I GCGC 2 cut(s) 973, 1173
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 5 cut(s) 317, 358, 467, 948, 1219
HpaII CCGG 1 cut(s) 1062
HphI GGTGA 5 cut(s) 469, 627, 829, 887, 980
Hpy166II GTNNAC 1 cut(s) 396
Hpy188I TCNGA 7 cut(s) 19, 609, 756, 801, 947, 1099, 1224
Hpy188III TCNNGA 4 cut(s) 218, 273, 702, 908
Hpy8I GTNNAC 1 cut(s) 396
HpyAV CCTTC 4 cut(s) 425, 924, 1196, 1242
HpyCH4III ACNGT 3 cut(s) 375, 845, 968
HpyCH4IV ACGT 2 cut(s) 1035, 1140
HpyCH4V TGCA 4 cut(s) 125, 197, 510, 786
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 591
HpyF3I CTNAG 7 cut(s) 228, 399, 700, 744, 798, 906, 989
HpySE526I ACGT 2 cut(s) 1035, 1140
Hsp92II CATG 6 cut(s) 44, 221, 292, 510, 874, 1076
HspAI GCGC 2 cut(s) 973, 1173
Ksp22I TGATCA 1 cut(s) 615
Kzo9I GATC 7 cut(s) 615, 704, 879, 910, 1123, 1143, 1278
LmnI GCTCC 2 cut(s) 856, 985
LpnPI CCDG 9 cut(s) 111, 164, 189, 311, 682, 687, 893, 903, 1075
Lsp1109I GCAGC 4 cut(s) 109, 522, 671, 757
LweI GCATC 3 cut(s) 184, 312, 795
MaeI CTAG 5 cut(s) 186, 191, 383, 497, 1286
MaeII ACGT 2 cut(s) 1035, 1140
MaeIII GTNAC 3 cut(s) 113, 412, 1031
MalI GATC 7 cut(s) 617, 706, 881, 912, 1125, 1145, 1280
MboI GATC 7 cut(s) 615, 704, 879, 910, 1123, 1143, 1278
MboII GAAGA 3 cut(s) 692, 1079, 1171
MfeI CAATTG 1 cut(s) 560
MflI RGATCY 4 cut(s) 704, 879, 910, 1278
MluCI AATT 7 cut(s) 58, 75, 256, 418, 442, 560, 896
MlyI GAGTC 3 cut(s) 311, 352, 942
MnlI CCTC 8 cut(s) 13, 98, 448, 539, 603, 743, 953, 1113
Mph1103I ATGCAT 1 cut(s) 199
MroXI GAANNNNTTC 2 cut(s) 244, 830
MseI TTAA 2 cut(s) 728, 1052
MspI CCGG 1 cut(s) 1062
MspR9I CCNGG 1 cut(s) 1063
MunI CAATTG 1 cut(s) 560
MvnI CGCG 2 cut(s) 1173, 1175
MwoI GCNNNNNNNGC 2 cut(s) 350, 591
NciI CCSGG 1 cut(s) 1063
NdeI CATATG 2 cut(s) 199, 977
NdeII GATC 7 cut(s) 615, 704, 879, 910, 1123, 1143, 1278
NlaIII CATG 6 cut(s) 44, 221, 292, 510, 874, 1076
NlaIV GGNNCC 6 cut(s) 182, 296, 405, 706, 912, 1280
NmuCI GTSAC 2 cut(s) 113, 1031
NsiI ATGCAT 1 cut(s) 199
NspV TTCGAA 1 cut(s) 54
PagI TCATGA 1 cut(s) 217
PceI AGGCCT 1 cut(s) 528
PdmI GAANNNNTTC 2 cut(s) 244, 830
PfeI GAWTC 2 cut(s) 467, 1219
PflFI GACNNNGTC 1 cut(s) 650
PfoI TCCNGGA 1 cut(s) 1061
PkrI GCNGC 4 cut(s) 124, 512, 661, 772
PleI GAGTC 3 cut(s) 311, 352, 942
PpsI GAGTC 3 cut(s) 311, 352, 942
Ppu21I YACGTR 1 cut(s) 1141
PpuMI RGGWCCY 1 cut(s) 403
Psp5II RGGWCCY 1 cut(s) 403
PspFI CCCAGC 1 cut(s) 325
PspN4I GGNNCC 6 cut(s) 182, 296, 405, 706, 912, 1280
PspPI GGNCC 1 cut(s) 403
PspPPI RGGWCCY 1 cut(s) 403
PsrI GAACNNNNNNTAC 2 cut(s) 987, 1019
PstI CTGCAG 1 cut(s) 127
PstNI CAGNNNCTG 1 cut(s) 613
PsuI RGATCY 4 cut(s) 704, 879, 910, 1278
PsyI GACNNNGTC 1 cut(s) 650
RsaI GTAC 2 cut(s) 377, 1215
RsaNI GTAC 2 cut(s) 376, 1214
SaqAI TTAA 2 cut(s) 728, 1052
SatI GCNGC 4 cut(s) 123, 511, 660, 771
Sau3AI GATC 7 cut(s) 615, 704, 879, 910, 1123, 1143, 1278
Sau96I GGNCC 1 cut(s) 403
ScaI AGTACT 1 cut(s) 377
SchI GAGTC 3 cut(s) 311, 352, 942
ScrFI CCNGG 1 cut(s) 1063
SfaNI GCATC 3 cut(s) 184, 312, 795
SfcI CTRYAG 1 cut(s) 123
SfuI TTCGAA 1 cut(s) 54
SinI GGWCC 1 cut(s) 403
SmlI CTYRAG 3 cut(s) 626, 883, 1091
SmoI CTYRAG 3 cut(s) 626, 883, 1091
Sse9I AATT 7 cut(s) 58, 75, 256, 418, 442, 560, 896
SseBI AGGCCT 1 cut(s) 528
SspI AATATT 1 cut(s) 279
SspMI CTAG 5 cut(s) 186, 191, 383, 497, 1286
StuI AGGCCT 1 cut(s) 528
StyD4I CCNGG 1 cut(s) 1061
TaaI ACNGT 3 cut(s) 375, 845, 968
TaiI ACGT 2 cut(s) 1038, 1143
TaqI TCGA 5 cut(s) 54, 265, 320, 453, 1205
TasI AATT 7 cut(s) 58, 75, 256, 418, 442, 560, 896
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 2 cut(s) 467, 1219
Tru1I TTAA 2 cut(s) 728, 1052
Tru9I TTAA 2 cut(s) 728, 1052
TseFI GTSAC 2 cut(s) 113, 1031
TseI GCWGC 4 cut(s) 122, 510, 659, 770
Tsp45I GTSAC 2 cut(s) 113, 1031
TspDTI ATGAA 9 cut(s) 57, 234, 476, 780, 819, 823, 1061, 1178, 1242
Tth111I GACNNNGTC 1 cut(s) 650
VpaK11BI GGWCC 1 cut(s) 403
XagI CCTNNNNNAGG 2 cut(s) 452, 905
XapI RAATTY 3 cut(s) 58, 418, 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XmnI GAANNNNTTC 2 cut(s) 244, 830
XspI CTAG 5 cut(s) 186, 191, 383, 497, 1286
ZrmI AGTACT 1 cut(s) 377
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.