AT5G59280

Arabidopsis Pumilio

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009111
Physical Location & Seq
Reverse (-)
23914069 .. 23915139
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G59280.1

Sequence Viewer

Length: 999 bp
ATGGCAAACAACAATTCCTTTTCAATGTCGACAATGTTCAATACTATACAAGAAATCCTGAATGAGGAGCCTTTGACAGTTCCTTCTCCTCCTTCACGTGGCGGCTATTCCTTATTCCACATCCGTAAAGCGGAGATGGATATTCAAAGGATTTTCAACTTCATGACTGGAAGTGAAGATTTGAAAGACGACATCTCAGTATTAGACACGGGAACGCTTATGTGGATGGCGTCTTTAATGACGTCTGACTGCGATTACTTCATGGTCATCACAACAAACAAGAACGGCTCCAAAACCCTACAGAAACTCATGGGAATGTCAGATGACATGGACGTCTTCTTCTTTGAGGCTATCATGCGCCTCTTCATCCATGTCATGACTGACAAGTACGCCTCCTACGTGACGATACAGGGGATGAGAGTTTTTCAACAGGACAAGAGAGAGTTGATGTATGATCAAATTCTTCGATACGCGTGTTTCCTGGCGGGTGACCAGTACGGTTGCATCGCGCTCAACGAAATCATAACCGACTTGGACGATCCTTACTACAGAGACCAGCTCCTTGACATAGTCGCGAACAACGCTCTTTTGCTAAGCAACGATGCTTATGGCAACTTTGTGGTCCAACACGTGCTTAAACTACGTGACTCCCGTTGCACGCGTAACATTGCTGATAAACTATGTGGCTACTGCGTCGAGCTCTCGTTTAAAAAGTATGGAAGCTACATCGTGGAGCGGCTTTTGGAGGCGGGGGACGTACCAATGGCAACAGTGGTTCTGGATCTGTTGGCGTGCAAGACAGAGATGTTGATAAGGTTGGCGAGGAGCGAGTACGGGAATTTTGTGGTGTGCAAGGCACTGGAATTAACAAATGAGATCCTCACGGCTGATCTGTTTTACGGTTTGGTGAATAGGCTCATGCCTTATCGCCATCTCTTGCATAGGTCTCCTCGAAGCAAGATTGCAACCATCTTGGACTCAATGAGTGTCCCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

332

Amino Acids

37.98

Weight (kDa)

5.13

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009111)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01410 AT1G21620 AT1G35850 AT1G35850 AT5G59280 AT5G60110 AT5G60180 AT5G64490
fragaria_vesca FvH4_3g00680
malus_domestica MD05G1360200.v1.1
prunus_persica Prupe.4G007700_v2.0.a1
pyrus_communis pycom05g32520
rosa_chinensis RchiOBHm_Chr5g0000911
rosa_laevigata RLG00000030906
rosa_roxburghii Rroxscaffold_1G00075340
rosa_rugosa Rorug04G0387800 Rorug04G0387900.1
rosa_samantha Rh5AG007400 Rh5BG009500 Rh5CG008100 Rh5DG008100
rosa_wichuraiana Rw5G000770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 332
AatII GACGTC 2 cut(s) 245, 336
AccBSI CCGCTC 1 cut(s) 736
AccI GTMKAC 1 cut(s) 29
AccII CGCG 4 cut(s) 473, 509, 575, 661
AciI CCGC 5 cut(s) 102, 131, 485, 736, 749
AclWI GGATC 3 cut(s) 533, 789, 871
AcsI RAATTY 2 cut(s) 459, 838
AcvI CACGTG 2 cut(s) 98, 631
AcyI GRCGYC 3 cut(s) 230, 242, 333
AfaI GTAC 4 cut(s) 389, 497, 759, 833
AfiI CCNNNNNNNGG 3 cut(s) 64, 98, 130
AflIII ACRYGT 3 cut(s) 471, 628, 659
AgsI TTSAA 6 cut(s) 24, 40, 146, 157, 184, 428
AjnI CCWGG 1 cut(s) 480
AluBI AGCT 3 cut(s) 559, 700, 723
AluI AGCT 3 cut(s) 559, 700, 723
Alw21I GWGCWC 1 cut(s) 702
Alw26I GTCTC 2 cut(s) 546, 951
AlwI GGATC 3 cut(s) 533, 789, 871
ApoI RAATTY 2 cut(s) 459, 838
AspLEI GCGC 2 cut(s) 360, 511
AspS9I GGNCC 1 cut(s) 622
AsuHPI GGTGA 2 cut(s) 500, 919
AvaII GGWCC 1 cut(s) 622
BaeI ACNNNNGTAYC 2 cut(s) 460, 493
BanII GRGCYC 1 cut(s) 702
BbrPI CACGTG 2 cut(s) 98, 631
BbsI GAAGAC 1 cut(s) 328
Bbv12I GWGCWC 1 cut(s) 702
BccI CCATC 4 cut(s) 130, 220, 939, 977
BceAI ACGGC 2 cut(s) 301, 900
BciT130I CCWGG 1 cut(s) 482
BclI TGATCA 1 cut(s) 454
BcoDI GTCTC 2 cut(s) 546, 951
BfaI CTAG 1 cut(s) 997
BfmI CTRYAG 2 cut(s) 299, 547
BisI GCNGC 2 cut(s) 103, 737
BlpI GCTNAGC 1 cut(s) 593
BlsI GCNGC 2 cut(s) 104, 738
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 1 cut(s) 622
BmgT120I GGNCC 1 cut(s) 622
BmiI GGNNCC 2 cut(s) 69, 289
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 2 cut(s) 513, 592
BpiI GAAGAC 1 cut(s) 328
BplI GAGNNNNNCTC 2 cut(s) 543, 575
Bpu1102I GCTNAGC 1 cut(s) 593
BsaAI YACGTR 4 cut(s) 98, 400, 631, 644
BsaHI GRCGYC 3 cut(s) 230, 242, 333
BsaI GGTCTC 2 cut(s) 546, 951
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 98, 130
Bse1I ACTGG 3 cut(s) 172, 493, 864
Bse3DI GCAATG 1 cut(s) 666
BseBI CCWGG 1 cut(s) 482
BseGI GGATG 4 cut(s) 120, 231, 366, 420
BseLI CCNNNNNNNGG 3 cut(s) 64, 98, 130
BseMI GCAATG 1 cut(s) 666
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 3 cut(s) 172, 493, 864
BseRI GAGGAG 4 cut(s) 78, 80, 838, 939
Bsh1236I CGCG 4 cut(s) 473, 509, 575, 661
BsiHKAI GWGCWC 1 cut(s) 702
BslFI GGGAC 2 cut(s) 767, 974
BslI CCNNNNNNNGG 3 cut(s) 64, 98, 130
BsmAI GTCTC 2 cut(s) 546, 951
BsmFI GGGAC 2 cut(s) 767, 974
Bso31I GGTCTC 2 cut(s) 546, 951
Bsp1286I GDGCHC 1 cut(s) 702
Bsp143I GATC 5 cut(s) 454, 538, 781, 876, 889
Bsp1720I GCTNAGC 1 cut(s) 593
Bsp68I TCGCGA 1 cut(s) 575
BspACI CCGC 5 cut(s) 102, 131, 485, 736, 749
BspCNI CTCAG 1 cut(s) 209
BspFNI CGCG 4 cut(s) 473, 509, 575, 661
BspHI TCATGA 2 cut(s) 162, 375
BspLI GGNNCC 2 cut(s) 69, 289
BspPI GGATC 3 cut(s) 533, 789, 871
BspTNI GGTCTC 2 cut(s) 546, 951
BsrBI CCGCTC 1 cut(s) 736
BsrDI GCAATG 1 cut(s) 666
BsrI ACTGG 3 cut(s) 172, 493, 864
BssMI GATC 5 cut(s) 454, 538, 781, 876, 889
BssNI GRCGYC 3 cut(s) 230, 242, 333
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 4 cut(s) 79, 500, 772, 902
Bst6I CTCTTC 1 cut(s) 368
BstACI GRCGYC 3 cut(s) 230, 242, 333
BstBAI YACGTR 4 cut(s) 98, 400, 631, 644
BstC8I GCNNGC 2 cut(s) 659, 793
BstDEI CTNAG 2 cut(s) 196, 593
BstEII GGTNACC 1 cut(s) 488
BstENI CCTNNNNNAGG 1 cut(s) 62
BstF5I GGATG 4 cut(s) 120, 231, 366, 420
BstFNI CGCG 4 cut(s) 473, 509, 575, 661
BstHHI GCGC 2 cut(s) 360, 511
BstKTI GATC 5 cut(s) 457, 541, 784, 879, 892
BstMAI GTCTC 2 cut(s) 546, 951
BstMBI GATC 5 cut(s) 454, 538, 781, 876, 889
BstMWI GCNNNNNNNGC 1 cut(s) 581
BstNI CCWGG 1 cut(s) 482
BstPI GGTNACC 1 cut(s) 488
BstSCI CCNGG 1 cut(s) 480
BstSFI CTRYAG 2 cut(s) 299, 547
BstUI CGCG 4 cut(s) 473, 509, 575, 661
BstV2I GAAGAC 1 cut(s) 328
BstX2I RGATCY 2 cut(s) 781, 876
BstYI RGATCY 2 cut(s) 781, 876
BtgZI GCGATG 1 cut(s) 490
BtsCI GGATG 4 cut(s) 120, 231, 366, 420
BtsIMutI CAGTG 2 cut(s) 777, 857
BtuMI TCGCGA 1 cut(s) 575
Cac8I GCNNGC 2 cut(s) 659, 793
CciI TCATGA 2 cut(s) 162, 375
CfoI GCGC 2 cut(s) 360, 511
Cfr13I GGNCC 1 cut(s) 622
CseI GACGC 2 cut(s) 219, 682
Csp6I GTAC 4 cut(s) 388, 496, 758, 832
CviAII CATG 8 cut(s) 163, 262, 310, 328, 355, 371, 376, 919
CviQI GTAC 4 cut(s) 388, 496, 758, 832
DdeI CTNAG 2 cut(s) 196, 593
DpnI GATC 5 cut(s) 456, 540, 783, 878, 891
DpnII GATC 5 cut(s) 454, 538, 781, 876, 889
DraI TTTAAA 1 cut(s) 709
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
Ecl136II GAGCTC 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 702
Eco31I GGTCTC 2 cut(s) 546, 951
Eco47I GGWCC 1 cut(s) 622
Eco53kI GAGCTC 1 cut(s) 700
Eco72I CACGTG 2 cut(s) 98, 631
Eco91I GGTNACC 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 700
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 480
EcoT38I GRGCYC 1 cut(s) 702
FaeI CATG 8 cut(s) 166, 265, 313, 331, 358, 374, 379, 922
FaqI GGGAC 2 cut(s) 767, 974
FatI CATG 8 cut(s) 162, 261, 309, 327, 354, 370, 375, 918
FauI CCCGC 2 cut(s) 478, 742
FbaI TGATCA 1 cut(s) 454
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 103, 737
FokI GGATG 4 cut(s) 107, 238, 353, 427
FriOI GRGCYC 1 cut(s) 702
Fsp4HI GCNGC 2 cut(s) 103, 737
FspBI CTAG 1 cut(s) 997
GlaI GCGC 2 cut(s) 359, 510
GluI GCNGC 2 cut(s) 103, 737
HgaI GACGC 2 cut(s) 219, 682
HhaI GCGC 2 cut(s) 360, 511
Hin1I GRCGYC 3 cut(s) 230, 242, 333
Hin1II CATG 8 cut(s) 166, 265, 313, 331, 358, 374, 379, 922
Hin6I GCGC 2 cut(s) 358, 509
HinP1I GCGC 2 cut(s) 358, 509
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 2 cut(s) 647, 977
HphI GGTGA 2 cut(s) 500, 919
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 2 cut(s) 247, 322
Hpy188III TCNNGA 5 cut(s) 58, 163, 376, 574, 779
Hpy8I GTNNAC 1 cut(s) 30
Hpy99I CGWCG 1 cut(s) 698
HpyAV CCTTC 2 cut(s) 93, 102
HpyCH4III ACNGT 4 cut(s) 79, 500, 772, 902
HpyCH4IV ACGT 7 cut(s) 97, 242, 333, 399, 630, 643, 756
HpyCH4V TGCA 6 cut(s) 504, 657, 795, 852, 940, 965
HpyF10VI GCNNNNNNNGC 1 cut(s) 581
HpyF3I CTNAG 2 cut(s) 196, 593
HpySE526I ACGT 7 cut(s) 97, 242, 333, 399, 630, 643, 756
Hsp92I GRCGYC 3 cut(s) 230, 242, 333
Hsp92II CATG 8 cut(s) 166, 265, 313, 331, 358, 374, 379, 922
HspAI GCGC 2 cut(s) 358, 509
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 5 cut(s) 454, 538, 781, 876, 889
LmnI GCTCC 5 cut(s) 67, 293, 564, 733, 825
LweI GCATC 2 cut(s) 513, 592
MaeI CTAG 1 cut(s) 997
MaeII ACGT 7 cut(s) 97, 242, 333, 399, 630, 643, 756
MaeIII GTNAC 4 cut(s) 400, 488, 644, 662
MalI GATC 5 cut(s) 456, 540, 783, 878, 891
MbiI CCGCTC 1 cut(s) 736
MboI GATC 5 cut(s) 454, 538, 781, 876, 889
MboII GAAGA 5 cut(s) 188, 328, 331, 355, 455
MflI RGATCY 2 cut(s) 781, 876
MhlI GDGCHC 1 cut(s) 702
MluCI AATT 4 cut(s) 13, 459, 838, 863
MluI ACGCGT 2 cut(s) 471, 659
MlyI GAGTC 2 cut(s) 641, 971
MmeI TCCRAC 1 cut(s) 649
MnlI CCTC 9 cut(s) 58, 99, 340, 371, 403, 739, 816, 890, 960
MseI TTAA 4 cut(s) 236, 636, 708, 866
MslI CAYNNNNRTG 1 cut(s) 314
MspR9I CCNGG 1 cut(s) 482
MvaI CCWGG 1 cut(s) 482
MvnI CGCG 4 cut(s) 473, 509, 575, 661
MwoI GCNNNNNNNGC 1 cut(s) 581
NdeII GATC 5 cut(s) 454, 538, 781, 876, 889
NlaIII CATG 8 cut(s) 166, 265, 313, 331, 358, 374, 379, 922
NlaIV GGNNCC 2 cut(s) 69, 289
NmuCI GTSAC 3 cut(s) 400, 488, 644
NruI TCGCGA 1 cut(s) 575
PagI TCATGA 2 cut(s) 162, 375
PcsI WCGNNNNNNNCGW 3 cut(s) 396, 513, 649
PflFI GACNNNGTC 1 cut(s) 569
PkrI GCNGC 2 cut(s) 104, 738
PleI GAGTC 2 cut(s) 641, 971
PmaCI CACGTG 2 cut(s) 98, 631
PmlI CACGTG 2 cut(s) 98, 631
PpsI GAGTC 2 cut(s) 641, 971
Ppu21I YACGTR 4 cut(s) 98, 400, 631, 644
Psp124BI GAGCTC 1 cut(s) 702
Psp6I CCWGG 1 cut(s) 480
PspCI CACGTG 2 cut(s) 98, 631
PspEI GGTNACC 1 cut(s) 488
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 2 cut(s) 69, 289
PspPI GGNCC 1 cut(s) 622
PsuI RGATCY 2 cut(s) 781, 876
PsyI GACNNNGTC 1 cut(s) 569
RruI TCGCGA 1 cut(s) 575
RsaI GTAC 4 cut(s) 389, 497, 759, 833
RsaNI GTAC 4 cut(s) 388, 496, 758, 832
RseI CAYNNNNRTG 1 cut(s) 314
SacI GAGCTC 1 cut(s) 702
SalI GTCGAC 1 cut(s) 28
SaqAI TTAA 4 cut(s) 236, 636, 708, 866
SatI GCNGC 2 cut(s) 103, 737
Sau3AI GATC 5 cut(s) 454, 538, 781, 876, 889
Sau96I GGNCC 1 cut(s) 622
SchI GAGTC 2 cut(s) 641, 971
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 1 cut(s) 702
SfaNI GCATC 2 cut(s) 513, 592
SfcI CTRYAG 2 cut(s) 299, 547
SinI GGWCC 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 314
Sse9I AATT 4 cut(s) 13, 459, 838, 863
SsiI CCGC 5 cut(s) 102, 131, 485, 736, 749
SspMI CTAG 1 cut(s) 997
SstI GAGCTC 1 cut(s) 702
StyD4I CCNGG 1 cut(s) 480
TaaI ACNGT 4 cut(s) 79, 500, 772, 902
TaiI ACGT 7 cut(s) 100, 245, 336, 402, 633, 646, 759
TaqI TCGA 4 cut(s) 29, 466, 696, 952
TasI AATT 4 cut(s) 13, 459, 838, 863
TauI GCSGC 2 cut(s) 105, 739
Tru1I TTAA 4 cut(s) 236, 636, 708, 866
Tru9I TTAA 4 cut(s) 236, 636, 708, 866
TscAI CASTG 2 cut(s) 777, 864
TseFI GTSAC 3 cut(s) 400, 488, 644
Tsp45I GTSAC 3 cut(s) 400, 488, 644
TspDTI ATGAA 3 cut(s) 151, 250, 355
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 2 cut(s) 777, 864
Tth111I GACNNNGTC 1 cut(s) 569
VpaK11BI GGWCC 1 cut(s) 622
XagI CCTNNNNNAGG 1 cut(s) 62
XapI RAATTY 2 cut(s) 459, 838
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 1 cut(s) 997
ZraI GACGTC 2 cut(s) 243, 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.