AT1G21650

Protein translocase subunit SECA2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013983
Physical Location & Seq
Reverse (-)
7592664 .. 7604229
11566 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G21650.1

Sequence Viewer

Length: 3156 bp
ATGGGTTCTGTTTCTAATCTTGTGAGCCCTAATATTTGCCATCCAGCTCCCCCTTGTCTCACATCTCGAAGTAATAAGTTTCCGTGGACAAAACCAATATCTGGTCTTTTGTTCTATCGGTCCGTCACGCCGATTAAGAGGTGTCACCTTGTTCGAAGAAGCTGTGTCGTCTCAGCTTCCCTAACGGGGAATTTGGGTCGTTTGAAGAGGAATGTACAAGATTTTACGAGCATGAATTATTGGGTTGTCCGGGACTACTACCGTCTGGTGGAATCTGTCAATTCCCTTGAACCACAGATTCAAAGCCTTTCCGATGAACAGCTGAAAGCTAAAACTGCTGAATTTCGAGAAAGATTAGCACGAGGGGAGTCCCTAGCAGATATGCAAGCTGAGGCATTTGCTGTTGTCCGTGAAGCTGCAAAGAGAACAATTGGAATGCGTCATTTTGACGTGCAGATAATTGGTGGAGGAGTGCTTCATGATGGATCCATTGCGGAGATGAAGACAGGTGAAGGGAAAACACTAGTTTCTACCTTAGCTGCATATCTTAATGCATTAACTGGTGAGGGCGTTCATGTGGTTACTGTGAATGATTACCTGGCACAGCGTGATGCTGAATGGATGGGTCGTGTTCACCGCTTCTTAGGCCTTTCAGTTGGTCTCATTCAGAGAGGGATGAAAGCTGAAGAGAGAAAATTCAACTATAGCTGTGATATAACATACACCAATAACTCGGAGCTTGGATTTGATTATCTACGAGATAACCTTACTTCAAACCGTGAACAACTTGTTATGAGATGGCCAAAACCATTTCACTTTGCAATAGTTGATGAAGTGGACTCAGTCCTCATTGATGAAGGCAGAAATCCTTTGCTAATTAGTGGTGAGGCTAACGAAAATGCCGCACGATATCCAGTTGCTGCTAAAGTGGCTGAACTTCTCGTAAAGGATAGTCATTACAAAGTTGAACTGAAAGAAAACTCAGTGGAACTCACAGAAGAAGGCATATCTCTTGCTGAAATGGCTCTTGAGACGGGTGATTTATGGGACGAAAATGATCCATGGGCAAGGTTTGTTATGAATGCTTTGAAAGCTAAAGAATTTTACAAGCGGGATGTCCAATATATTGTGAGAGATGGGAAAGCACTTATAATCAATGAGTTGACTGGAAGAGTTGAAGACAAAAGGAGATGGTCTGAAGGAGTTCACCAGGCTGTGGAGGCTAAAGAAGGTCTAGAAATTCAGGCTGATTCAATTGTTGTGGCTCAAATCACCTATCAGTCGCTCTTTAAACTCTATCCAAAGCTCTCCGGGATGACTGGGACAGCAAAAACAGAAGAAAAAGAATTTTTGAAAATGTTTCAGATACCTGTTATTGAAGTTCCTACAAATTTGTCAAATATTCGAATCGATCTACCAATACAAGCTTTCGCGACTGCTCGGGGTAAATGGGAACATGTTCGTCGAGAAGTTGAAGATATGTTTGGACAAGGTCGACCTGTTTTAGTAGGAACAACAAGTGTAGAGAATTCTGAATATTTATCAGAACTGCTGAAAGAATGGGGTATACCTCACAATGTCCTGAATGCACGACCCAAGTATGCTGCCAGGGAAGCTGATTTTATAGCCCAAGCAGGAAGAAAATATGCCATCACCATTTCCACAAATATGGCTGGTAGGGGTACTGACATTATTCTGGGAGGAAATCCGAAGATGCTTGCAAGGGAAATCATAGAAGATAGCATCCTTTCATATCTAACGAGTGAAGTTTTGGCTGATAATATTGACGATGATGAGTTATCACAGAAGGTATTGTCAAAAATAAAAGTAGGACCATCGTCATTAGCTTTGCTAGCCAGAGCTTCTCTTATGGCAAAATATGTTGGCAAAAGTGAAAGTAAGAGCTGGACAAGGAAAAAAGCAAAGTCCGTGGTCACAGAATCTTTGGAGAAAAGCCAGACCATGGATCCTATGGAACTGCAGAATCTTATCAATGAGCAGTCAGAGATGTACCCATTAGGCCCTGCTATTGCTCTGGCTTATCTGTCCGTCTTAAAGGACTGTGAGGCCCATTGTCTGCATGAAGGGTCTGAAGTGAAGAGGCTTGGAGGCCTTCATGTGATTGGGACTTCATTGCATGAGTCTCGGAGAATTGATAACCAGCTTCGTGGCAGAGCTGGAAGGCAAGGAGATCCTGGATCCACGCGGTTTATGATAAGCTTGCAAGATGAGATGTTTCAGAAGTTTAATTTTGATACTGAGTGGGCTGTGAGACTTATATCAAAGATTACAAACGATGAAGACTTACCAATTGAAGGTGACACAATAGTAAAACAGCTTCTAGCTCTCCAGATCAACGCAGAGAAATACTTCTTTGGCATAAGAAAAAGCCTGGTCGAGTTTGACGAAGTGCTAGAGGTTCAAAGGAAGCATGTCTATGACTTAAGGCAACTGCTGTTGACGGGTGAAAACGAAAGTTGCTCTCAGCATATATTCCAGTACATGCAAGCTGTTGTAGATGAAATCGTTGTTGGGAATTCTAATCCGCAGAAGCATCCAAGATACTGGAGCTTGGCCAAGTTATTGAAAGAGTTCATGGCTATTTCGGGAAACCTACTGGATGAAGAGACCATGTTACAGTCCCTTGAAAACCTGCATGAGGGAAGTTCAATAGAAATGGAAGACTTGTCCCTTCCGCACCTGCCAAAACCTCCAAACGCTTTCAGAGGAATTCGCAGGAAGAATTCTTCACTAAGACGTTGGCTTGATATCTGCTCCGATAATTTGACGGGGAGTGGGAGTTACCGAACATTGATTAATCTTCTCCGGAAGTTCCTAGGTGATTATCTGATTGCTTCATACTTAAATGTTGTTCAAGAATCTGGCTTTGATGATGGATACATAAAAGAAATAGAGAGAGCAGTTCTTTTAAAGACGTTGGATTGCTACTGGAGAGATCATCTTGTGAACATGAATAAACTAAGCTCAGCGGTGAATGTCAGAAGTTTTGCGCATAGAAACCCTCTAGAAGAATATAAAATCGATGGATGTCGGTTTTTCATCTCAATGCTTAGCGCAACTAGAAGGCTAACCGTAGAATCAATCTTGCAGTACTGGTCATCCCCGATGGAATCCCAAGAACTATTCATATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko02024 ko03060 ko03070 map02024 map03060 map03070
Pfam Domains
Protein Families
SecA_DEAD SecA_PP_bind SecA_SW

Protein Analysis

1051

Amino Acids

118.93

Weight (kDa)

6.09

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecA_DEAD PF07517 80 - 450 2.5e-119 SecA DEAD-like domain
SecA_PP_bind PF01043 301 - 407 7.2e-31 SecA preprotein cross-linking domain
P-loop_SecA PF21090 468 - 753 2.4e-65 SecA P-loop domain
SecA_SW PF07516 770 - 1037 4.3e-42 SecA Wing and Scaffold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013983)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21650 AT1G21650 AT1G21650
fragaria_vesca FvH4_2g27750
malus_domestica MD00G1100600.v1.1
prunus_persica Prupe.1G490400_v2.0.a1
pyrus_communis pycom15g30570
rosa_chinensis RchiOBHm_Chr6g0296621
rosa_laevigata RLG00000011676
rosa_multiflora Rmu_sc0001741.1_g000019
rosa_roxburghii Rroxscaffold_7G00171330 Rroxscaffold_7G00178770
rosa_rugosa Rorug06G0263700
rosa_samantha Rh6AG375100 Rh6CG388700
rosa_wichuraiana Rw6G032720 Rw6G032810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1151
AarI CACCTGC 1 cut(s) 2709
Acc16I TGCGCA 1 cut(s) 3012
Acc36I ACCTGC 2 cut(s) 2661, 2709
AccB7I CCANNNNNTGG 3 cut(s) 101, 1216, 1963
AccI GTMKAC 2 cut(s) 1495, 1567
AccII CGCG 2 cut(s) 1433, 2206
AccIII TCCGGA 1 cut(s) 2826
AciI CCGC 8 cut(s) 494, 637, 903, 1111, 2206, 2546, 2696, 2990
AclWI GGATC 8 cut(s) 480, 493, 1052, 1961, 1974, 2186, 2193, 2206
AcoI YGGCCR 2 cut(s) 800, 2574
AcuI CTGAAG 3 cut(s) 705, 1218, 2112
AdeI CACNNNGTG 1 cut(s) 608
AfaI GTAC 5 cut(s) 216, 1684, 2012, 2501, 3113
AfiI CCNNNNNNNGG 7 cut(s) 101, 186, 268, 1216, 1963, 2315, 2659
AflII CTTAAG 1 cut(s) 2443
AflIII ACRYGT 1 cut(s) 1456
AhlI ACTAGT 1 cut(s) 523
AjiI CACGTC 1 cut(s) 451
AjnI CCWGG 5 cut(s) 597, 1209, 1607, 2194, 2391
AjuI GAANNNNNNNTTGG 4 cut(s) 1467, 1499, 2514, 2546
Alw26I GTCTC 7 cut(s) 62, 175, 665, 1025, 2148, 2266, 2621
AlwI GGATC 8 cut(s) 480, 493, 1052, 1961, 1974, 2186, 2193, 2206
AlwNI CAGNNNCTG 1 cut(s) 920
Ama87I CYCGRG 1 cut(s) 1440
Aor13HI TCCGGA 1 cut(s) 2826
AoxI GGCC 6 cut(s) 646, 800, 2020, 2067, 2110, 2574
ApeKI GCWGC 4 cut(s) 416, 539, 920, 1604
AseI ATTAAT 1 cut(s) 2817
Asp700I GAANNNNTTC 4 cut(s) 1203, 1458, 2369, 2591
AspA2I CCTAGG 1 cut(s) 2836
AspLEI GCGC 2 cut(s) 3013, 3077
AspS9I GGNCC 4 cut(s) 120, 1832, 2021, 2068
AsuC2I CCSGG 2 cut(s) 251, 1312
AsuII TTCGAA 2 cut(s) 154, 1405
AsuNHI GCTAGC 1 cut(s) 1852
AvaI CYCGRG 1 cut(s) 1440
AvaII GGWCC 2 cut(s) 120, 1832
AvrII CCTAGG 1 cut(s) 2836
BalI TGGCCA 2 cut(s) 802, 2576
BamHI GGATCC 3 cut(s) 485, 1966, 2198
BanII GRGCYC 1 cut(s) 29
BarI GAAGNNNNNNTAC 2 cut(s) 2322, 2354
BauI CACGAG 1 cut(s) 360
BbsI GAAGAC 4 cut(s) 509, 1185, 2307, 2688
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 4 cut(s) 403, 526, 907, 1591
BcgI CGANNNNNNTGC 2 cut(s) 2126, 2160
BciT130I CCWGG 5 cut(s) 599, 1211, 1609, 2196, 2393
BciVI GTATCC 1 cut(s) 2891
BcnI CCSGG 2 cut(s) 251, 1312
BcoDI GTCTC 7 cut(s) 62, 175, 665, 1025, 2148, 2266, 2621
BcuI ACTAGT 1 cut(s) 523
BfaI CTAG 9 cut(s) 374, 524, 1235, 1853, 2342, 2414, 2837, 3026, 3081
BfmI CTRYAG 2 cut(s) 703, 1979
BfrI CTTAAG 1 cut(s) 2443
BfuAI ACCTGC 2 cut(s) 2661, 2709
BfuI GTATCC 1 cut(s) 2891
BisI GCNGC 5 cut(s) 417, 540, 903, 921, 1605
BlnI CCTAGG 1 cut(s) 2836
BlpI GCTNAGC 2 cut(s) 2986, 3071
BlsI GCNGC 5 cut(s) 418, 541, 904, 922, 1606
BmcAI AGTACT 1 cut(s) 3113
Bme1390I CCNGG 7 cut(s) 251, 599, 1211, 1312, 1609, 2196, 2393
Bme18I GGWCC 2 cut(s) 120, 1832
BmeT110I CYCGRG 1 cut(s) 1440
BmgBI CACGTC 1 cut(s) 451
BmgT120I GGNCC 4 cut(s) 120, 1832, 2021, 2068
BmiI GGNNCC 3 cut(s) 487, 1968, 2200
BmrFI CCNGG 7 cut(s) 251, 599, 1211, 1312, 1609, 2196, 2393
BmrI ACTGGG 1 cut(s) 1329
BmsI GCATC 4 cut(s) 601, 1704, 1752, 2563
BmtI GCTAGC 1 cut(s) 1856
BmuI ACTGGG 1 cut(s) 1329
BoxI GACNNNNGTC 1 cut(s) 1837
BpiI GAAGAC 4 cut(s) 509, 1185, 2307, 2688
BpmI CTGGAG 3 cut(s) 2333, 2587, 2971
Bpu10I CCTNAGC 2 cut(s) 390, 535
Bpu1102I GCTNAGC 2 cut(s) 2986, 3071
Bpu14I TTCGAA 2 cut(s) 154, 1405
BpuEI CTTGAG 1 cut(s) 1049
BpuMI CCSGG 2 cut(s) 251, 1312
Bsa29I ATCGAT 2 cut(s) 1410, 3042
BsaI GGTCTC 2 cut(s) 665, 2621
BsaJI CCNNGG 6 cut(s) 83, 1061, 1608, 1929, 1962, 2836
BsaWI WCCGGW 1 cut(s) 2826
BsaXI ACNNNNNCTCC 2 cut(s) 2785, 2815
Bsc4I CCNNNNNNNGG 7 cut(s) 101, 186, 268, 1216, 1963, 2315, 2659
Bse1I ACTGG 9 cut(s) 565, 914, 1171, 1324, 2497, 2570, 2622, 2954, 3119
Bse3DI GCAATG 2 cut(s) 489, 2132
BseAI TCCGGA 1 cut(s) 2826
BseBI CCWGG 5 cut(s) 599, 1211, 1609, 2196, 2393
BseCI ATCGAT 2 cut(s) 1410, 3042
BseDI CCNNGG 6 cut(s) 83, 1061, 1608, 1929, 1962, 2836
BseLI CCNNNNNNNGG 7 cut(s) 101, 186, 268, 1216, 1963, 2315, 2659
BseMI GCAATG 2 cut(s) 489, 2132
BseMII CTCAG 7 cut(s) 186, 381, 855, 996, 2250, 2498, 3000
BseNI ACTGG 9 cut(s) 565, 914, 1171, 1324, 2497, 2570, 2622, 2954, 3119
BseRI GAGGAG 1 cut(s) 483
BseXI GCAGC 4 cut(s) 403, 526, 907, 1591
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 2 cut(s) 1433, 2206
BshFI GGCC 6 cut(s) 648, 802, 2022, 2069, 2112, 2576
BshVI ATCGAT 2 cut(s) 1410, 3042
BsiHKCI CYCGRG 1 cut(s) 1440
BsiSI CCGG 3 cut(s) 250, 1311, 2827
BslFI GGGAC 7 cut(s) 266, 355, 1061, 1336, 2140, 2626, 2674
BslI CCNNNNNNNGG 7 cut(s) 101, 186, 268, 1216, 1963, 2315, 2659
BsmAI GTCTC 7 cut(s) 62, 175, 665, 1025, 2148, 2266, 2621
BsmBI CGTCTC 2 cut(s) 175, 1025
BsmFI GGGAC 7 cut(s) 266, 355, 1061, 1336, 2140, 2626, 2674
BsmI GAATGC 3 cut(s) 441, 1087, 1591
BsnI GGCC 6 cut(s) 648, 802, 2022, 2069, 2112, 2576
Bso31I GGTCTC 2 cut(s) 665, 2621
BsoBI CYCGRG 1 cut(s) 1440
Bsp119I TTCGAA 2 cut(s) 154, 1405
Bsp1286I GDGCHC 1 cut(s) 29
Bsp13I TCCGGA 1 cut(s) 2826
Bsp1407I TGTACA 1 cut(s) 214
Bsp143I GATC 8 cut(s) 485, 1057, 1411, 1966, 2191, 2198, 2352, 2956
Bsp1720I GCTNAGC 2 cut(s) 2986, 3071
Bsp19I CCATGG 2 cut(s) 1061, 1962
Bsp68I TCGCGA 1 cut(s) 1433
BspACI CCGC 8 cut(s) 494, 637, 903, 1111, 2206, 2546, 2696, 2990
BspANI GGCC 6 cut(s) 648, 802, 2022, 2069, 2112, 2576
BspCNI CTCAG 7 cut(s) 185, 382, 854, 995, 2251, 2497, 2999
BspDI ATCGAT 2 cut(s) 1410, 3042
BspEI TCCGGA 1 cut(s) 2826
BspFNI CGCG 2 cut(s) 1433, 2206
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 3 cut(s) 487, 1968, 2200
BspMAI CTGCAG 1 cut(s) 1983
BspMI ACCTGC 2 cut(s) 2661, 2709
BspOI GCTAGC 1 cut(s) 1856
BspPI GGATC 8 cut(s) 480, 493, 1052, 1961, 1974, 2186, 2193, 2206
BspT104I TTCGAA 2 cut(s) 154, 1405
BspTI CTTAAG 1 cut(s) 2443
BspTNI GGTCTC 2 cut(s) 665, 2621
BsrDI GCAATG 2 cut(s) 489, 2132
BsrGI TGTACA 1 cut(s) 214
BsrI ACTGG 9 cut(s) 565, 914, 1171, 1324, 2497, 2570, 2622, 2954, 3119
BssECI CCNNGG 6 cut(s) 83, 1061, 1608, 1929, 1962, 2836
BssMI GATC 8 cut(s) 485, 1057, 1411, 1966, 2191, 2198, 2352, 2956
BssNAI GTATAC 1 cut(s) 1568
BssSI CACGAG 1 cut(s) 360
BssT1I CCWWGG 3 cut(s) 1061, 1962, 2836
Bst1107I GTATAC 1 cut(s) 1568
Bst2BI CACGAG 1 cut(s) 360
Bst2UI CCWGG 5 cut(s) 599, 1211, 1609, 2196, 2393
Bst4CI ACNGT 6 cut(s) 263, 586, 779, 2063, 2640, 3094
Bst6I CTCTTC 5 cut(s) 200, 681, 1165, 2093, 2619
BstAFI CTTAAG 1 cut(s) 2443
BstAUI TGTACA 1 cut(s) 214
BstBI TTCGAA 2 cut(s) 154, 1405
BstC8I GCNNGC 5 cut(s) 387, 1719, 1854, 2222, 2508
BstDSI CCRYGG 4 cut(s) 83, 1061, 1929, 1962
BstENI CCTNNNNNAGG 1 cut(s) 2657
BstFNI CGCG 2 cut(s) 1433, 2206
BstHHI GCGC 2 cut(s) 3013, 3077
BstKTI GATC 8 cut(s) 488, 1060, 1414, 1969, 2194, 2201, 2355, 2959
BstMAI GTCTC 7 cut(s) 62, 175, 665, 1025, 2148, 2266, 2621
BstMBI GATC 8 cut(s) 485, 1057, 1411, 1966, 2191, 2198, 2352, 2956
BstMWI GCNNNNNNNGC 8 cut(s) 335, 645, 929, 1022, 1091, 1220, 1613, 1853
BstNI CCWGG 5 cut(s) 599, 1211, 1609, 2196, 2393
BstNSI RCATGY 3 cut(s) 1460, 2435, 2506
BstPAI GACNNNNGTC 1 cut(s) 1837
BstSCI CCNGG 7 cut(s) 249, 597, 1209, 1310, 1607, 2194, 2391
BstSFI CTRYAG 2 cut(s) 703, 1979
BstUI CGCG 2 cut(s) 1433, 2206
BstV1I GCAGC 4 cut(s) 403, 526, 907, 1591
BstV2I GAAGAC 4 cut(s) 509, 1185, 2307, 2688
BstX2I RGATCY 4 cut(s) 485, 1966, 2191, 2198
BstXI CCANNNNNNTGG 3 cut(s) 1669, 2168, 2565
BstYI RGATCY 4 cut(s) 485, 1966, 2191, 2198
BstZ17I GTATAC 1 cut(s) 1568
Bsu15I ATCGAT 2 cut(s) 1410, 3042
BsuI GTATCC 1 cut(s) 2891
BsuRI GGCC 6 cut(s) 648, 802, 2022, 2069, 2112, 2576
BsuTUI ATCGAT 2 cut(s) 1410, 3042
BtgI CCRYGG 4 cut(s) 83, 1061, 1929, 1962
BtrI CACGTC 1 cut(s) 451
BtsIMutI CAGTG 1 cut(s) 990
BtuMI TCGCGA 1 cut(s) 1433
BveI ACCTGC 2 cut(s) 2661, 2709
Cac8I GCNNGC 5 cut(s) 387, 1719, 1854, 2222, 2508
CaiI CAGNNNCTG 1 cut(s) 920
CciI TCATGA 1 cut(s) 478
CfoI GCGC 2 cut(s) 3013, 3077
Cfr13I GGNCC 4 cut(s) 120, 1832, 2021, 2068
ClaI ATCGAT 2 cut(s) 1410, 3042
CpoI CGGWCCG 1 cut(s) 120
CseI GACGC 1 cut(s) 428
Csp6I GTAC 5 cut(s) 215, 1683, 2011, 2500, 3112
CspCI CAANNNNNGTGG 4 cut(s) 1242, 1277, 1911, 1946
CspI CGGWCCG 1 cut(s) 120
CviQI GTAC 5 cut(s) 215, 1683, 2011, 2500, 3112
DpnI GATC 8 cut(s) 487, 1059, 1413, 1968, 2193, 2200, 2354, 2958
DpnII GATC 8 cut(s) 485, 1057, 1411, 1966, 2191, 2198, 2352, 2956
DraI TTTAAA 2 cut(s) 1291, 2931
DraIII CACNNNGTG 1 cut(s) 608
EaeI YGGCCR 2 cut(s) 800, 2574
Eam1104I CTCTTC 5 cut(s) 200, 681, 1165, 2093, 2619
EarI CTCTTC 5 cut(s) 200, 681, 1165, 2093, 2619
Eco130I CCWWGG 3 cut(s) 1061, 1962, 2836
Eco147I AGGCCT 2 cut(s) 648, 2112
Eco24I GRGCYC 1 cut(s) 29
Eco31I GGTCTC 2 cut(s) 665, 2621
Eco32I GATATC 2 cut(s) 911, 2770
Eco47I GGWCC 2 cut(s) 120, 1832
Eco57I CTGAAG 3 cut(s) 705, 1218, 2112
Eco88I CYCGRG 1 cut(s) 1440
EcoNI CCTNNNNNAGG 1 cut(s) 2657
EcoO109I RGGNCCY 1 cut(s) 2021
EcoRI GAATTC 4 cut(s) 1528, 2536, 2730, 2743
EcoRII CCWGG 5 cut(s) 597, 1209, 1607, 2194, 2391
EcoRV GATATC 2 cut(s) 911, 2770
EcoT14I CCWWGG 3 cut(s) 1061, 1962, 2836
EcoT22I ATGCAT 1 cut(s) 556
EcoT38I GRGCYC 1 cut(s) 29
ErhI CCWWGG 3 cut(s) 1061, 1962, 2836
Esp3I CGTCTC 2 cut(s) 175, 1025
FaqI GGGAC 7 cut(s) 266, 355, 1061, 1336, 2140, 2626, 2674
FauI CCCGC 1 cut(s) 1104
FblI GTMKAC 2 cut(s) 1495, 1567
Fnu4HI GCNGC 5 cut(s) 417, 540, 903, 921, 1605
FriOI GRGCYC 1 cut(s) 29
Fsp4HI GCNGC 5 cut(s) 417, 540, 903, 921, 1605
FspBI CTAG 9 cut(s) 374, 524, 1235, 1853, 2342, 2414, 2837, 3026, 3081
FspI TGCGCA 1 cut(s) 3012
GlaI GCGC 2 cut(s) 3012, 3076
GluI GCNGC 5 cut(s) 417, 540, 903, 921, 1605
GsuI CTGGAG 3 cut(s) 2333, 2587, 2971
HaeIII GGCC 6 cut(s) 648, 802, 2022, 2069, 2112, 2576
HapII CCGG 3 cut(s) 250, 1311, 2827
HgaI GACGC 1 cut(s) 428
HhaI GCGC 2 cut(s) 3013, 3077
Hin6I GCGC 2 cut(s) 3011, 3075
HinP1I GCGC 2 cut(s) 3011, 3075
HincII GTYRAC 3 cut(s) 1164, 1496, 2460
HindII GTYRAC 3 cut(s) 1164, 1496, 2460
HindIII AAGCTT 2 cut(s) 1425, 2218
HpaII CCGG 3 cut(s) 250, 1311, 2827
Hpy99I CGWCG 1 cut(s) 1467
HpyCH4III ACNGT 6 cut(s) 263, 586, 779, 2063, 2640, 3094
HpyCH4IV ACGT 3 cut(s) 450, 2758, 2936
HpyF10VI GCNNNNNNNGC 8 cut(s) 335, 645, 929, 1022, 1091, 1220, 1613, 1853
HpySE526I ACGT 3 cut(s) 450, 2758, 2936
HspAI GCGC 2 cut(s) 3011, 3075
Kpn2I TCCGGA 1 cut(s) 2826
Kzo9I GATC 8 cut(s) 485, 1057, 1411, 1966, 2191, 2198, 2352, 2956
LmnI GCTCC 4 cut(s) 52, 736, 2568, 2780
Lsp1109I GCAGC 4 cut(s) 403, 526, 907, 1591
LweI GCATC 4 cut(s) 601, 1704, 1752, 2563
MaeI CTAG 9 cut(s) 374, 524, 1235, 1853, 2342, 2414, 2837, 3026, 3081
MaeII ACGT 3 cut(s) 450, 2758, 2936
MaeIII GTNAC 7 cut(s) 124, 143, 580, 1933, 2318, 2634, 2801
MalI GATC 8 cut(s) 487, 1059, 1413, 1968, 2193, 2200, 2354, 2958
MboI GATC 8 cut(s) 485, 1057, 1411, 1966, 2191, 2198, 2352, 2956
MfeI CAATTG 3 cut(s) 429, 1254, 2310
MflI RGATCY 4 cut(s) 485, 1966, 2191, 2198
MhlI GDGCHC 1 cut(s) 29
MlsI TGGCCA 2 cut(s) 802, 2576
MluNI TGGCCA 2 cut(s) 802, 2576
MlyI GAGTC 3 cut(s) 377, 833, 2150
MmeI TCCRAC 1 cut(s) 2919
Mox20I TGGCCA 2 cut(s) 802, 2576
Mph1103I ATGCAT 1 cut(s) 556
MroI TCCGGA 1 cut(s) 2826
MroXI GAANNNNTTC 4 cut(s) 1203, 1458, 2369, 2591
MscI TGGCCA 2 cut(s) 802, 2576
MslI CAYNNNNRTG 3 cut(s) 1667, 2436, 3065
Msp20I TGGCCA 2 cut(s) 802, 2576
MspA1I CMGCKG 2 cut(s) 322, 2990
MspCI CTTAAG 1 cut(s) 2443
MspI CCGG 3 cut(s) 250, 1311, 2827
MspR9I CCNGG 7 cut(s) 251, 599, 1211, 1312, 1609, 2196, 2393
MunI CAATTG 3 cut(s) 429, 1254, 2310
Mva1269I GAATGC 3 cut(s) 441, 1087, 1591
MvaI CCWGG 5 cut(s) 599, 1211, 1609, 2196, 2393
MvnI CGCG 2 cut(s) 1433, 2206
MwoI GCNNNNNNNGC 8 cut(s) 335, 645, 929, 1022, 1091, 1220, 1613, 1853
NciI CCSGG 2 cut(s) 251, 1312
NcoI CCATGG 2 cut(s) 1061, 1962
NdeII GATC 8 cut(s) 485, 1057, 1411, 1966, 2191, 2198, 2352, 2956
NheI GCTAGC 1 cut(s) 1852
NlaIV GGNNCC 3 cut(s) 487, 1968, 2200
NmuCI GTSAC 4 cut(s) 124, 143, 1933, 2318
NruI TCGCGA 1 cut(s) 1433
NsbI TGCGCA 1 cut(s) 3012
NsiI ATGCAT 1 cut(s) 556
NspI RCATGY 3 cut(s) 1460, 2435, 2506
NspV TTCGAA 2 cut(s) 154, 1405
PagI TCATGA 1 cut(s) 478
PaqCI CACCTGC 1 cut(s) 2709
PceI AGGCCT 2 cut(s) 648, 2112
PciI ACATGT 1 cut(s) 1456
PcsI WCGNNNNNNNCGW 1 cut(s) 2403
PctI GAATGC 3 cut(s) 441, 1087, 1591
PdmI GAANNNNTTC 4 cut(s) 1203, 1458, 2369, 2591
PfeI GAWTC 9 cut(s) 272, 298, 1250, 1407, 1940, 1984, 2879, 3098, 3131
PflFI GACNNNGTC 2 cut(s) 842, 1491
PflMI CCANNNNNTGG 3 cut(s) 101, 1216, 1963
PfoI TCCNGGA 3 cut(s) 249, 1310, 2194
PkrI GCNGC 5 cut(s) 418, 541, 904, 922, 1606
PleI GAGTC 3 cut(s) 376, 833, 2149
PpsI GAGTC 3 cut(s) 376, 833, 2149
PscI ACATGT 1 cut(s) 1456
PshAI GACNNNNGTC 1 cut(s) 1837
PshBI ATTAAT 1 cut(s) 2817
PsiI TTATAA 1 cut(s) 1151
Psp6I CCWGG 5 cut(s) 597, 1209, 1607, 2194, 2391
PspGI CCWGG 5 cut(s) 597, 1209, 1607, 2194, 2391
PspN4I GGNNCC 3 cut(s) 487, 1968, 2200
PspPI GGNCC 4 cut(s) 120, 1832, 2021, 2068
PstI CTGCAG 1 cut(s) 1983
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 4 cut(s) 485, 1966, 2191, 2198
PsyI GACNNNGTC 2 cut(s) 842, 1491
PvuII CAGCTG 1 cut(s) 322
RruI TCGCGA 1 cut(s) 1433
RsaI GTAC 5 cut(s) 216, 1684, 2012, 2501, 3113
RsaNI GTAC 5 cut(s) 215, 1683, 2011, 2500, 3112
RseI CAYNNNNRTG 3 cut(s) 1667, 2436, 3065
Rsr2I CGGWCCG 1 cut(s) 120
RsrII CGGWCCG 1 cut(s) 120
SalI GTCGAC 1 cut(s) 1494
SatI GCNGC 5 cut(s) 417, 540, 903, 921, 1605
Sau3AI GATC 8 cut(s) 485, 1057, 1411, 1966, 2191, 2198, 2352, 2956
Sau96I GGNCC 4 cut(s) 120, 1832, 2021, 2068
ScaI AGTACT 1 cut(s) 3113
SchI GAGTC 3 cut(s) 377, 833, 2150
ScrFI CCNGG 7 cut(s) 251, 599, 1211, 1312, 1609, 2196, 2393
SduI GDGCHC 1 cut(s) 29
SfaNI GCATC 4 cut(s) 601, 1704, 1752, 2563
SfcI CTRYAG 2 cut(s) 703, 1979
SfuI TTCGAA 2 cut(s) 154, 1405
SinI GGWCC 2 cut(s) 120, 1832
SmiMI CAYNNNNRTG 3 cut(s) 1667, 2436, 3065
SmlI CTYRAG 2 cut(s) 1028, 2443
SmoI CTYRAG 2 cut(s) 1028, 2443
SpeI ACTAGT 1 cut(s) 523
SseBI AGGCCT 2 cut(s) 648, 2112
SsiI CCGC 8 cut(s) 494, 637, 903, 1111, 2206, 2546, 2696, 2990
SspI AATATT 4 cut(s) 34, 1402, 1538, 1783
SspMI CTAG 9 cut(s) 374, 524, 1235, 1853, 2342, 2414, 2837, 3026, 3081
StuI AGGCCT 2 cut(s) 648, 2112
StyD4I CCNGG 7 cut(s) 249, 597, 1209, 1310, 1607, 2194, 2391
StyI CCWWGG 3 cut(s) 1061, 1962, 2836
TaaI ACNGT 6 cut(s) 263, 586, 779, 2063, 2640, 3094
TaiI ACGT 3 cut(s) 453, 2761, 2939
TaqI TCGA 9 cut(s) 67, 154, 346, 1405, 1410, 1465, 1495, 2397, 3042
TaqII GACCGA 1 cut(s) 108
TatI WGTACW 3 cut(s) 214, 2499, 3111
TauI GCSGC 1 cut(s) 905
TfiI GAWTC 9 cut(s) 272, 298, 1250, 1407, 1940, 1984, 2879, 3098, 3131
TscAI CASTG 1 cut(s) 990
TseFI GTSAC 4 cut(s) 124, 143, 1933, 2318
TseI GCWGC 4 cut(s) 416, 539, 920, 1604
Tsp45I GTSAC 4 cut(s) 124, 143, 1933, 2318
TspGWI ACGGA 5 cut(s) 72, 112, 398, 1918, 2038
TspRI CASTG 1 cut(s) 990
Tth111I GACNNNGTC 2 cut(s) 842, 1491
Van91I CCANNNNNTGG 3 cut(s) 101, 1216, 1963
Vha464I CTTAAG 1 cut(s) 2443
VpaK11BI GGWCC 2 cut(s) 120, 1832
VspI ATTAAT 1 cut(s) 2817
XagI CCTNNNNNAGG 1 cut(s) 2657
XbaI TCTAGA 2 cut(s) 1234, 3025
XceI RCATGY 3 cut(s) 1460, 2435, 2506
XcmI CCANNNNNNNNNTGG 1 cut(s) 1969
XmaJI CCTAGG 1 cut(s) 2836
XmiI GTMKAC 2 cut(s) 1495, 1567
XmnI GAANNNNTTC 4 cut(s) 1203, 1458, 2369, 2591
XspI CTAG 9 cut(s) 374, 524, 1235, 1853, 2342, 2414, 2837, 3026, 3081
ZrmI AGTACT 1 cut(s) 3113
Zsp2I ATGCAT 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.