Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0013983

Physical Location & Seq

Genomic Coordinates Forward (+)

21947812 .. 21956542

Gene Structure 8731 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g27750.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3144 bp

ATGGCTTCTACTTCCCTCCAAATCCCCACTTTCCTCTCCCTCAAACCACTTCCTCCACGCGCCACCTTCGCCTTCCCCACCTCCTTCTCTTCCTTCCCCGCCTCCTCTCCCCCTCCACGCCGCCGTCTCACTCTCTCTTCGGCCTCCCTCAAGGAAAATCTCGGCCGGCTTGGCAAGACATGGAACGACGTCACTAGCCTCAACAGCTGGGTTGTCCATGACTATTACCGCCTTGTCAGCTCTGTCAACTCCTTTGAGCCTCAGCTGCAGCGCCTCACTGATGACCAGCTTACTGCTAAAACTGCCGAGTTCCGGCGGCGATTGCGACAGGGAGAGACGCTTGCCGATATCCAAGCAGAGGCTTTTGCTGTTGTTCGTGAAGCTGCGAAACGGAAGCTTGGTATGCGGCATTTTGATGTTCAGATTATTGGCGGGGCGGTGCTTCATGATGGCTCCATTGCCGAAATGAAGACGGGAGAGGGGAAGACGTTGGTTTCCACTTTAGCTGCTTATCTTAACGCGCTGACTGGCGAAGGTGTTCATGTGGTAACCGTAAATGATTACCTTGCTCAAAGAGATGCTGAGTGGATGGGTCGTGTTCATCGCTTCTTAGGTCTTTCCGTGGGTCTTATTCAGAGAGGGATGACATCTGACAAGAGGAGATCCAACTATAGATGTGACATAACATACACCAATAATTCAGAACTTGGTTTTGACTATCTACGAGACAATCTTGCCGGAAACAGTGGAGAAGTTGTTATGAGATCGCCAAAGCCATTTCATTTTGCAATAGTTGATGAAGTTGACTCAGTTCTTATCGATGAAGGAAGAAATCCATTGCTAATTAGTGGCGAGGCTAGTAAAGATGCAGCACGCTATCCTGTTGCTGCTAAAGTGGCTGAGTTGCTTGTGCGAGGAATTCATTACACAGTGGAACTTAAAGATTACGCAGTTGAGTTGACTGAAGAAGGAATAGCTCTTGCTGAGATGGCTCTTGAAACAAATGACTTATGGGATGAAAATGATCCCTGGGCCAGATTTGTCATGAATGCGCTGAAAGCTAAAGAGTTCTATCGGAAGGATGTGCAATATATTGTCAGAAATGGGAAGGCTCTCATAATCAATGAGCTCACTGGCAGAGTCGAAGATAAGAGAAGGTGGTCTGAGGGTATTCATCAAGCTGTGGAGGGTAAAGAAGGTTTGAAGATTCAGGCGGATTCAGTAGTTATCGCACAAATCACATATCAATCTCTTTTCAAGCTCTACCCAAAGCTATCAGGGATGACTGGGACTGCAAAAACTGAAGAGAAGGAGTTCTTGAAAATGTTCCAAACACCAGTTATTGAAGTGCCAACAAATTTGCCAAACATTCGTAATGATTTACCTGTTCAAGCTTTCGCGACTGCTCAGGGGAAATGGGAATATGTTCGTCAAGAAGTGGAATACATGTTCAGGCAGGGGCGACCTGTTTTGGTTGGCACCACTAGTGTTGAGCATTCTGAACACCTGTCTGATCTGCTGCGGGAACATAACATCCCACACAATGTTCTAAATGCACGACCCAAGTATGCTGCAAAGGAAGCTGAAATTGTTGCTCAAGCTGGACGGAAGCATGCCATTACTCTTTCTACCAATATGGCTGGCAGAGGCACTGACATAATACTGGGAGGGAATCCTAAAATGCTTGCGAAAGAGATTATAGAAGACAGTTTGATTTCATCCCTGACACGAGAAGCTCCTGACATTGACATAGATGGAGAGGCAATTTCACAAAAGGTATTGTCAAAGATAAAGGTCGGTCCATCATCAATAGCTTTGCTGGCTAAGACGGCATTAATGGCTAAATATGTTGGCAAAAATGAGGGGAAGAGCTGGACATATAAGGAGGCAAAAGCCATGATATCAGAGTCTGTGGAAATGAGTCAATCAAAAGATATGAAAGAGCTAGAGAAGCTTGTTGATGAACAGTCTGAGATGTACCCTCTCGGCCCTACAATAGCACTTGCTTATCTTTCAGTTTTAAAGGATTGCGAGGTACACTGTTTCAAAGAAGGGTCTGAAGTGAAAAGACTTGGGGGCCTTCACGTGATTGGAACATCTTTGCACGAGTCTCGTAGAATAGATAACCAGCTTCGTGGCAGAGCAGGAAGGCAAGGTGACCCAGGGTCAACTCGATTTATGGTGAGCTTGCAGGATGAGATGTTTCAAAAGTTCAATTTTGATACTGAGTGGGCTGTGAGACTTATTTCAAAAATTACTGATGATGAGGATGTACCAATTGAAGGTGATGCCATTGTGAGACAGCTCCTGTCCCTGCAAATTAATGCAGAAAAGTACTTTTTTGGAATAAGAAAGAGCCTGGTTGAGTTTGATGAAGTATTAGAGGTGCAGAGAAAGCATGTGTATGAACTTAGGCAGTCTATTTTAACCGGTGATAATGAAAGTTGTGCCCAGCTCGTATTCCAGTACATGCAAGCAGTGGCAGATGAAATTGTCTTTAAAAATGTTGATGCCCTCAAGCATCCAAGGAACTGGAGTTTGAATAAGCTTTTAACGGAGTATGTGGAAATTGCCGGGAAACTATTGGATGATTCATTTGCAGAAATTACTGAGGAAGCTTTGTTAAAATCTCTGGCACAGTCACCTGAACTGAACTATAAAGAAATTGATGACATTCACCTTCCAAACTTGCCAAGACCTCCAAATGCCTTCAGAGGTATCCGCAAGAAGAACTCTTCATTGAAACGTTGGCTTGCTATATGCTCGGACGATTTGACCAAGAATGGAAGGTACCATGCTACTACTAACCTCCTCCGCAAGTACCTTGGAGACTATCTGATTGCTTCATATCTGGATGTTGTTCAAGACTCTGGTTATGATGATACCTATGTGAAGGAAGTTGAAAGAGCAGTTATTGTCAAAACCCTTGACTGTTTCTGGAGGGATCACCTTGTGAACATGAACAGGCTAAGCTCAGCGGTGAATGTAAGGAGTTTTGGGCACAGGAATCCTCTTGAAGAATACAAAATTGATGGTTGTCGATTTTTCATATCTATGCTTAGTGCAACGCGAAGGTTAACTGTAGAGTCACTTCTGAAATATTGGTCCTCCCCAATGGAGTCTCAAGAAATTTTTGTATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0006996 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0009657 GO:0009658 GO:0009987 GO:0010109 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019222 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0031323 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW WD40 zf-RING_UBOX

Protein Analysis

1048

Amino Acids

118.11

Weight (kDa)

6.33

Isoelectric Point (pI)

39.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	71 - 439	1.1e-116	SecA DEAD-like domain
SecA_PP_bind	PF01043	290 - 396	4.7e-29	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	455 - 742	4.5e-66	SecA P-loop domain
SecA_SW	PF07516	759 - 1034	1e-40	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21650 AT1G21650 AT1G21650
fragaria_vesca	FvH4_2g27750
malus_domestica	MD00G1100600.v1.1
prunus_persica	Prupe.1G490400_v2.0.a1
pyrus_communis	pycom15g30570
rosa_chinensis	RchiOBHm_Chr6g0296621
rosa_laevigata	RLG00000011676
rosa_multiflora	Rmu_sc0001741.1_g000019
rosa_roxburghii	Rroxscaffold_7G00171330 Rroxscaffold_7G00178770
rosa_rugosa	Rorug06G0263700
rosa_samantha	Rh6AG375100 Rh6CG388700
rosa_wichuraiana	Rw6G032720 Rw6G032810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	192
Acc65I	GGTACC	1 cut(s)	2790
AccB1I	GGYRCC	2 cut(s)	1478, 2790
AccII	CGCG	4 cut(s)	60, 521, 1400, 3070
AclI	AACGTT	1 cut(s)	2746
AclWI	GGATC	3 cut(s)	657, 1019, 2952
AcoI	YGGCCR	1 cut(s)	163
AcsI	RAATTY	3 cut(s)	918, 1357, 3129
AcuI	CTGAAG	4 cut(s)	984, 1323, 2079, 2695
AcvI	CACGTG	1 cut(s)	2086
AcyI	GRCGYC	1 cut(s)	189
AdeI	CACNNNGTG	1 cut(s)	2953
AfaI	GTAC	7 cut(s)	1979, 2037, 2274, 2336, 2468, 2792, 2822
AfiI	CCNNNNNNNGG	3 cut(s)	312, 358, 2282
AflIII	ACRYGT	1 cut(s)	1446
AgeI	ACCGGT	1 cut(s)	2429
AhdI	GACNNNNNGTC	1 cut(s)	2164
AhlI	ACTAGT	1 cut(s)	1484
AjnI	CCWGG	3 cut(s)	1028, 2161, 2358
AloI	GAACNNNNNNTCC	4 cut(s)	1433, 1465, 1518, 1550
Alw21I	GWGCWC	1 cut(s)	1131
Alw26I	GTCTC	8 cut(s)	131, 329, 720, 2115, 2233, 2293, 2823, 3126
AlwI	GGATC	3 cut(s)	657, 1019, 2952
AlwNI	CAGNNNCTG	2 cut(s)	1910, 2308
AoxI	GGCC	5 cut(s)	141, 163, 1032, 1987, 2077
ApeKI	GCWGC	8 cut(s)	265, 268, 383, 506, 869, 887, 1519, 1571
ApoI	RAATTY	3 cut(s)	918, 1357, 3129
AseI	ATTAAT	2 cut(s)	1835, 2322
AsiGI	ACCGGT	1 cut(s)	2429
Asp700I	GAANNNNTTC	4 cut(s)	537, 1313, 1325, 1425
Asp718I	GGTACC	1 cut(s)	2790
AspLEI	GCGC	4 cut(s)	62, 273, 523, 1054
AspS9I	GGNCC	5 cut(s)	1032, 1799, 1988, 2077, 3105
AsuC2I	CCSGG	1 cut(s)	2575
AsuHPI	GGTGA	8 cut(s)	2168, 2194, 2297, 2444, 2634, 2669, 2939, 2992
AvaII	GGWCC	2 cut(s)	1799, 3105
BaeGI	GKGCMC	2 cut(s)	2452, 3003
BaeI	ACNNNNGTAYC	2 cut(s)	2874, 2907
BanI	GGYRCC	2 cut(s)	1478, 2790
BanII	GRGCYC	1 cut(s)	1131
BarI	GAAGNNNNNNTAC	2 cut(s)	3075, 3107
BauI	CACGAG	2 cut(s)	1728, 2105
BbrPI	CACGTG	1 cut(s)	2086
BbsI	GAAGAC	3 cut(s)	476, 491, 1710
Bbv12I	GWGCWC	1 cut(s)	1131
BbvCI	CCTCAGC	1 cut(s)	261
BbvI	GCAGC	8 cut(s)	252, 280, 370, 493, 874, 881, 1506, 1558
BccI	CCATC	6 cut(s)	443, 583, 982, 1748, 1810, 3026
BceAI	ACGGC	2 cut(s)	108, 1845
BcgI	CGANNNNNNTGC	2 cut(s)	2093, 2127
BciT130I	CCWGG	3 cut(s)	1030, 2163, 2360
BciVI	GTATCC	1 cut(s)	2729
BcnI	CCSGG	1 cut(s)	2575
BcoDI	GTCTC	8 cut(s)	131, 329, 720, 2115, 2233, 2293, 2823, 3126
BcuI	ACTAGT	1 cut(s)	1484
BfaI	CTAG	4 cut(s)	195, 858, 1485, 1946
BfmI	CTRYAG	3 cut(s)	266, 670, 3081
BfoI	RGCGCY	1 cut(s)	274
BfuI	GTATCC	1 cut(s)	2729
BglI	GCCNNNNNGGC	1 cut(s)	171
BlpI	GCTNAGC	2 cut(s)	2969, 2974
BmcAI	AGTACT	1 cut(s)	2336
Bme1390I	CCNGG	4 cut(s)	1030, 2163, 2360, 2575
Bme18I	GGWCC	2 cut(s)	1799, 3105
BmeRI	GACNNNNNGTC	1 cut(s)	2164
BmgT120I	GGNCC	5 cut(s)	1032, 1799, 1988, 2077, 3105
BmiI	GGNNCC	4 cut(s)	454, 1480, 2078, 2792
BmrFI	CCNGG	4 cut(s)	1030, 2163, 2360, 2575
BmrI	ACTGGG	2 cut(s)	1296, 1673
BmsI	GCATC	5 cut(s)	568, 856, 2278, 2500, 2530
BmuI	ACTGGG	2 cut(s)	1296, 1673
BpiI	GAAGAC	3 cut(s)	476, 491, 1710
BpmI	CTGGAG	2 cut(s)	2554, 2959
Bpu10I	CCTNAGC	2 cut(s)	261, 1407
Bpu1102I	GCTNAGC	2 cut(s)	2969, 2974
BpuEI	CTTGAG	4 cut(s)	134, 1581, 2501, 3108
BpuMI	CCSGG	1 cut(s)	2575
Bsa29I	ATCGAT	1 cut(s)	819
BsaAI	YACGTR	1 cut(s)	2086
BsaHI	GRCGYC	1 cut(s)	189
BsaJI	CCNNGG	7 cut(s)	621, 1028, 1029, 2161, 2162, 2525, 2824
BsaWI	WCCGGW	1 cut(s)	2429
BsaXI	ACNNNNNCTCC	2 cut(s)	741, 771
Bsc4I	CCNNNNNNNGG	3 cut(s)	312, 358, 2282
Bse118I	RCCGGY	2 cut(s)	165, 2429
Bse1I	ACTGG	7 cut(s)	532, 1138, 1291, 1337, 1668, 2464, 2537
Bse3DI	GCAATG	2 cut(s)	456, 836
BseBI	CCWGG	3 cut(s)	1030, 2163, 2360
BseCI	ATCGAT	1 cut(s)	819
BseDI	CCNNGG	7 cut(s)	621, 1028, 1029, 2161, 2162, 2525, 2824
BseLI	CCNNNNNNNGG	3 cut(s)	312, 358, 2282
BseMI	GCAATG	2 cut(s)	456, 836
BseNI	ACTGG	7 cut(s)	532, 1138, 1291, 1337, 1668, 2464, 2537
BseRI	GAGGAG	3 cut(s)	94, 673, 2801
BseSI	GKGCMC	2 cut(s)	2452, 3003
BseX3I	CGGCCG	1 cut(s)	163
BseXI	GCAGC	8 cut(s)	252, 280, 370, 493, 874, 881, 1506, 1558
BseYI	CCCAGC	2 cut(s)	207, 2451
BsgI	GTGCAG	1 cut(s)	2408
Bsh1236I	CGCG	4 cut(s)	60, 521, 1400, 3070
Bsh1285I	CGRYCG	1 cut(s)	166
BshFI	GGCC	5 cut(s)	143, 165, 1034, 1989, 2079
BshNI	GGYRCC	2 cut(s)	1478, 2790
BshTI	ACCGGT	1 cut(s)	2429
BshVI	ATCGAT	1 cut(s)	819
BsiEI	CGRYCG	1 cut(s)	166
BsiHKAI	GWGCWC	1 cut(s)	1131
BsiSI	CCGG	5 cut(s)	166, 313, 738, 2430, 2574
BslFI	GGGAC	2 cut(s)	1303, 2296
BslI	CCNNNNNNNGG	3 cut(s)	312, 358, 2282
BsmAI	GTCTC	8 cut(s)	131, 329, 720, 2115, 2233, 2293, 2823, 3126
BsmBI	CGTCTC	2 cut(s)	131, 329
BsmFI	GGGAC	2 cut(s)	1303, 2296
BsmI	GAATGC	2 cut(s)	1054, 1495
BsnI	GGCC	5 cut(s)	143, 165, 1034, 1989, 2079
Bsp1286I	GDGCHC	3 cut(s)	1131, 2452, 3003
Bsp143I	GATC	5 cut(s)	662, 764, 1024, 1513, 2944
Bsp1720I	GCTNAGC	2 cut(s)	2969, 2974
Bsp68I	TCGCGA	1 cut(s)	1400
BspANI	GGCC	5 cut(s)	143, 165, 1034, 1989, 2079
BspDI	ATCGAT	1 cut(s)	819
BspFNI	CGCG	4 cut(s)	60, 521, 1400, 3070
BspHI	TCATGA	2 cut(s)	445, 1044
BspLI	GGNNCC	4 cut(s)	454, 1480, 2078, 2792
BspMAI	CTGCAG	1 cut(s)	270
BspPI	GGATC	3 cut(s)	657, 1019, 2952
BspQI	GCTCTTC	1 cut(s)	1862
BspT107I	GGYRCC	2 cut(s)	1478, 2790
BsrDI	GCAATG	2 cut(s)	456, 836
BsrFI	RCCGGY	2 cut(s)	165, 2429
BsrI	ACTGG	7 cut(s)	532, 1138, 1291, 1337, 1668, 2464, 2537
BssAI	RCCGGY	2 cut(s)	165, 2429
BssECI	CCNNGG	7 cut(s)	621, 1028, 1029, 2161, 2162, 2525, 2824
BssMI	GATC	5 cut(s)	662, 764, 1024, 1513, 2944
BssNI	GRCGYC	1 cut(s)	189
BssSI	CACGAG	2 cut(s)	1728, 2105
BssT1I	CCWWGG	2 cut(s)	2525, 2824
Bst2BI	CACGAG	2 cut(s)	1728, 2105
Bst2UI	CCWGG	3 cut(s)	1030, 2163, 2360
Bst4CI	ACNGT	9 cut(s)	553, 746, 931, 1709, 1968, 2042, 2640, 2933, 3082
Bst6I	CTCTTC	5 cut(s)	94, 142, 1299, 1862, 2740
BstACI	GRCGYC	1 cut(s)	189
BstBAI	YACGTR	1 cut(s)	2086
BstDSI	CCRYGG	1 cut(s)	621
BstEII	GGTNACC	2 cut(s)	547, 2156
BstFNI	CGCG	4 cut(s)	60, 521, 1400, 3070
BstH2I	RGCGCY	1 cut(s)	274
BstHHI	GCGC	4 cut(s)	62, 273, 523, 1054
BstKTI	GATC	5 cut(s)	665, 767, 1027, 1516, 2947
BstMAI	GTCTC	8 cut(s)	131, 329, 720, 2115, 2233, 2293, 2823, 3126
BstMBI	GATC	5 cut(s)	662, 764, 1024, 1513, 2944
BstMCI	CGRYCG	1 cut(s)	166
BstNI	CCWGG	3 cut(s)	1030, 2163, 2360
BstNSI	RCATGY	4 cut(s)	1450, 1616, 2402, 2473
BstPI	GGTNACC	2 cut(s)	547, 2156
BstSCI	CCNGG	4 cut(s)	1028, 2161, 2358, 2573
BstSFI	CTRYAG	3 cut(s)	266, 670, 3081
BstSLI	GKGCMC	2 cut(s)	2452, 3003
BstUI	CGCG	4 cut(s)	60, 521, 1400, 3070
BstV1I	GCAGC	8 cut(s)	252, 280, 370, 493, 874, 881, 1506, 1558
BstV2I	GAAGAC	3 cut(s)	476, 491, 1710
BstX2I	RGATCY	1 cut(s)	662
BstXI	CCANNNNNNTGG	2 cut(s)	2135, 2532
BstYI	RGATCY	1 cut(s)	662
BstZI	CGGCCG	1 cut(s)	163
Bsu15I	ATCGAT	1 cut(s)	819
BsuI	GTATCC	1 cut(s)	2729
BsuRI	GGCC	5 cut(s)	143, 165, 1034, 1989, 2079
BsuTUI	ATCGAT	1 cut(s)	819
BtgI	CCRYGG	1 cut(s)	621
BtgZI	GCGATG	1 cut(s)	587
BtsI	GCAGTG	1 cut(s)	2484
BtsIMutI	CAGTG	7 cut(s)	276, 751, 936, 1131, 1650, 2038, 2484
BtuMI	TCGCGA	1 cut(s)	1400
CaiI	CAGNNNCTG	2 cut(s)	1910, 2308
CciI	TCATGA	2 cut(s)	445, 1044
CfoI	GCGC	4 cut(s)	62, 273, 523, 1054
Cfr10I	RCCGGY	2 cut(s)	165, 2429
Cfr13I	GGNCC	5 cut(s)	1032, 1799, 1988, 2077, 3105
ClaI	ATCGAT	1 cut(s)	819
CseI	GACGC	1 cut(s)	346
Csp6I	GTAC	7 cut(s)	1978, 2036, 2273, 2335, 2467, 2791, 2821
CspAI	ACCGGT	1 cut(s)	2429
CspCI	CAANNNNNGTGG	2 cut(s)	1471, 1506
CviQI	GTAC	7 cut(s)	1978, 2036, 2273, 2335, 2467, 2791, 2821
DpnI	GATC	5 cut(s)	664, 766, 1026, 1515, 2946
DpnII	GATC	5 cut(s)	662, 764, 1024, 1513, 2944
DraI	TTTAAA	2 cut(s)	2022, 2500
DraIII	CACNNNGTG	1 cut(s)	2953
DriI	GACNNNNNGTC	1 cut(s)	2164
EaeI	YGGCCR	1 cut(s)	163
EagI	CGGCCG	1 cut(s)	163
Eam1104I	CTCTTC	5 cut(s)	94, 142, 1299, 1862, 2740
Eam1105I	GACNNNNNGTC	1 cut(s)	2164
EarI	CTCTTC	5 cut(s)	94, 142, 1299, 1862, 2740
EciI	GGCGGA	1 cut(s)	1229
Ecl136II	GAGCTC	1 cut(s)	1129
EclXI	CGGCCG	1 cut(s)	163
Eco130I	CCWWGG	2 cut(s)	2525, 2824
Eco24I	GRGCYC	1 cut(s)	1131
Eco32I	GATATC	2 cut(s)	349, 1902
Eco47I	GGWCC	2 cut(s)	1799, 3105
Eco52I	CGGCCG	1 cut(s)	163
Eco53kI	GAGCTC	1 cut(s)	1129
Eco57I	CTGAAG	4 cut(s)	984, 1323, 2079, 2695
Eco72I	CACGTG	1 cut(s)	2086
Eco91I	GGTNACC	2 cut(s)	547, 2156
EcoICRI	GAGCTC	1 cut(s)	1129
EcoO109I	RGGNCCY	1 cut(s)	2077
EcoO65I	GGTNACC	2 cut(s)	547, 2156
EcoRI	GAATTC	1 cut(s)	918
EcoRII	CCWGG	3 cut(s)	1028, 2161, 2358
EcoRV	GATATC	2 cut(s)	349, 1902
EcoT14I	CCWWGG	2 cut(s)	2525, 2824
EcoT38I	GRGCYC	1 cut(s)	1131
ErhI	CCWWGG	2 cut(s)	2525, 2824
Esp3I	CGTCTC	2 cut(s)	131, 329
FalI	AAGNNNNNCTT	2 cut(s)	1301, 1333
FaqI	GGGAC	2 cut(s)	1303, 2296
FauI	CCCGC	3 cut(s)	106, 425, 1515
FriOI	GRGCYC	1 cut(s)	1131
FspBI	CTAG	4 cut(s)	195, 858, 1485, 1946
GlaI	GCGC	4 cut(s)	61, 272, 522, 1053
GsaI	CCCAGC	2 cut(s)	211, 2455
GsuI	CTGGAG	2 cut(s)	2554, 2959
HaeII	RGCGCY	1 cut(s)	274
HaeIII	GGCC	5 cut(s)	143, 165, 1034, 1989, 2079
HapII	CCGG	5 cut(s)	166, 313, 738, 2430, 2574
HgaI	GACGC	1 cut(s)	346
HhaI	GCGC	4 cut(s)	62, 273, 523, 1054
Hin1I	GRCGYC	1 cut(s)	189
Hin6I	GCGC	4 cut(s)	60, 271, 521, 1052
HinP1I	GCGC	4 cut(s)	60, 271, 521, 1052
HincII	GTYRAC	5 cut(s)	247, 805, 960, 2169, 3078
HindII	GTYRAC	5 cut(s)	247, 805, 960, 2169, 3078
HindIII	AAGCTT	5 cut(s)	395, 1392, 1952, 2546, 2616
HpaI	GTTAAC	1 cut(s)	3078
HpaII	CCGG	5 cut(s)	166, 313, 738, 2430, 2574
HphI	GGTGA	8 cut(s)	2168, 2194, 2297, 2444, 2634, 2669, 2939, 2992
Hpy166II	GTNNAC	7 cut(s)	247, 805, 960, 2038, 2169, 2956, 3078
Hpy8I	GTNNAC	7 cut(s)	247, 805, 960, 2038, 2169, 2956, 3078
Hpy99I	CGWCG	1 cut(s)	191
HpyCH4III	ACNGT	9 cut(s)	553, 746, 931, 1709, 1968, 2042, 2640, 2933, 3082
HpyCH4IV	ACGT	4 cut(s)	189, 488, 2085, 2746
HpySE526I	ACGT	4 cut(s)	189, 488, 2085, 2746
Hsp92I	GRCGYC	1 cut(s)	189
HspAI	GCGC	4 cut(s)	60, 271, 521, 1052
KpnI	GGTACC	1 cut(s)	2794
KroI	GCCGGC	1 cut(s)	165
KroNI	GCCGGC	1 cut(s)	167
KspAI	GTTAAC	1 cut(s)	3078
Kzo9I	GATC	5 cut(s)	662, 764, 1024, 1513, 2944
LguI	GCTCTTC	1 cut(s)	1862
LmnI	GCTCC	3 cut(s)	458, 1741, 2310
Lsp1109I	GCAGC	8 cut(s)	252, 280, 370, 493, 874, 881, 1506, 1558
LweI	GCATC	5 cut(s)	568, 856, 2278, 2500, 2530
MaeI	CTAG	4 cut(s)	195, 858, 1485, 1946
MaeII	ACGT	4 cut(s)	189, 488, 2085, 2746
MaeIII	GTNAC	6 cut(s)	190, 547, 677, 2156, 2640, 3087
MalI	GATC	5 cut(s)	664, 766, 1026, 1515, 2946
MboI	GATC	5 cut(s)	662, 764, 1024, 1513, 2944
MfeI	CAATTG	1 cut(s)	2277
MflI	RGATCY	1 cut(s)	662
MhlI	GDGCHC	3 cut(s)	1131, 2452, 3003
MlyI	GAGTC	8 cut(s)	800, 1149, 1916, 1930, 2117, 2861, 3095, 3128
MmeI	TCCRAC	1 cut(s)	690
MroNI	GCCGGC	1 cut(s)	165
MroXI	GAANNNNTTC	4 cut(s)	537, 1313, 1325, 1425
MslI	CAYNNNNRTG	3 cut(s)	414, 2403, 3053
MspA1I	CMGCKG	3 cut(s)	207, 265, 2978
MspI	CCGG	5 cut(s)	166, 313, 738, 2430, 2574
MspR9I	CCNGG	4 cut(s)	1030, 2163, 2360, 2575
MunI	CAATTG	1 cut(s)	2277
Mva1269I	GAATGC	2 cut(s)	1054, 1495
MvaI	CCWGG	3 cut(s)	1030, 2163, 2360
MvnI	CGCG	4 cut(s)	60, 521, 1400, 3070
NaeI	GCCGGC	1 cut(s)	167
NciI	CCSGG	1 cut(s)	2575
NdeII	GATC	5 cut(s)	662, 764, 1024, 1513, 2944
NgoMIV	GCCGGC	1 cut(s)	165
NlaIV	GGNNCC	4 cut(s)	454, 1480, 2078, 2792
NmeAIII	GCCGAG	3 cut(s)	141, 331, 1965
NmuCI	GTSAC	5 cut(s)	190, 677, 2156, 2640, 3087
NruI	TCGCGA	1 cut(s)	1400
NspI	RCATGY	4 cut(s)	1450, 1616, 2402, 2473
PaeI	GCATGC	1 cut(s)	1616
PagI	TCATGA	2 cut(s)	445, 1044
PasI	CCCWGGG	2 cut(s)	1029, 2162
PciI	ACATGT	1 cut(s)	1446
PciSI	GCTCTTC	1 cut(s)	1862
PctI	GAATGC	2 cut(s)	1054, 1495
PdiI	GCCGGC	1 cut(s)	167
PdmI	GAANNNNTTC	4 cut(s)	537, 1313, 1325, 1425
PfeI	GAWTC	5 cut(s)	1207, 1217, 1672, 2591, 3007
PinAI	ACCGGT	1 cut(s)	2429
PleI	GAGTC	8 cut(s)	800, 1148, 1915, 1929, 2116, 2861, 3094, 3127
PmaCI	CACGTG	1 cut(s)	2086
PmlI	CACGTG	1 cut(s)	2086
PpsI	GAGTC	8 cut(s)	800, 1148, 1915, 1929, 2116, 2861, 3094, 3127
Ppu21I	YACGTR	1 cut(s)	2086
PscI	ACATGT	1 cut(s)	1446
PshBI	ATTAAT	2 cut(s)	1835, 2322
Psp124BI	GAGCTC	1 cut(s)	1131
Psp1406I	AACGTT	1 cut(s)	2746
Psp6I	CCWGG	3 cut(s)	1028, 2161, 2358
PspCI	CACGTG	1 cut(s)	2086
PspEI	GGTNACC	2 cut(s)	547, 2156
PspFI	CCCAGC	2 cut(s)	207, 2451
PspGI	CCWGG	3 cut(s)	1028, 2161, 2358
PspN4I	GGNNCC	4 cut(s)	454, 1480, 2078, 2792
PspPI	GGNCC	5 cut(s)	1032, 1799, 1988, 2077, 3105
PstI	CTGCAG	1 cut(s)	270
PstNI	CAGNNNCTG	2 cut(s)	1910, 2308
PsuI	RGATCY	1 cut(s)	662
PvuII	CAGCTG	2 cut(s)	207, 265
RruI	TCGCGA	1 cut(s)	1400
RsaI	GTAC	7 cut(s)	1979, 2037, 2274, 2336, 2468, 2792, 2822
RsaNI	GTAC	7 cut(s)	1978, 2036, 2273, 2335, 2467, 2791, 2821
RseI	CAYNNNNRTG	3 cut(s)	414, 2403, 3053
SacI	GAGCTC	1 cut(s)	1131
SapI	GCTCTTC	1 cut(s)	1862
Sau3AI	GATC	5 cut(s)	662, 764, 1024, 1513, 2944
Sau96I	GGNCC	5 cut(s)	1032, 1799, 1988, 2077, 3105
ScaI	AGTACT	1 cut(s)	2336
SchI	GAGTC	8 cut(s)	800, 1149, 1916, 1930, 2117, 2861, 3095, 3128
ScrFI	CCNGG	4 cut(s)	1030, 2163, 2360, 2575
SduI	GDGCHC	3 cut(s)	1131, 2452, 3003
SfaNI	GCATC	5 cut(s)	568, 856, 2278, 2500, 2530
SfcI	CTRYAG	3 cut(s)	266, 670, 3081
SinI	GGWCC	2 cut(s)	1799, 3105
SmiMI	CAYNNNNRTG	3 cut(s)	414, 2403, 3053
SmlI	CTYRAG	4 cut(s)	149, 1596, 2516, 3123
SmoI	CTYRAG	4 cut(s)	149, 1596, 2516, 3123
SpeI	ACTAGT	1 cut(s)	1484
SphI	GCATGC	1 cut(s)	1616
SspI	AATATT	1 cut(s)	3101
SspMI	CTAG	4 cut(s)	195, 858, 1485, 1946
SstI	GAGCTC	1 cut(s)	1131
StyD4I	CCNGG	4 cut(s)	1028, 2161, 2358, 2573
StyI	CCWWGG	2 cut(s)	2525, 2824
TaaI	ACNGT	9 cut(s)	553, 746, 931, 1709, 1968, 2042, 2640, 2933, 3082
TaiI	ACGT	4 cut(s)	192, 491, 2088, 2749
TaqI	TCGA	4 cut(s)	819, 1143, 2173, 3040
TaqII	GACCGA	1 cut(s)	1787
TatI	WGTACW	2 cut(s)	2334, 2466
TauI	GCSGC	3 cut(s)	123, 319, 409
TfiI	GAWTC	5 cut(s)	1207, 1217, 1672, 2591, 3007
TscAI	CASTG	7 cut(s)	283, 751, 936, 1138, 1657, 2045, 2484
TseFI	GTSAC	5 cut(s)	190, 677, 2156, 2640, 3087
TseI	GCWGC	8 cut(s)	265, 268, 383, 506, 869, 887, 1519, 1571
Tsp45I	GTSAC	5 cut(s)	190, 677, 2156, 2640, 3087
TspGWI	ACGGA	4 cut(s)	406, 610, 1621, 2570
TspRI	CASTG	7 cut(s)	283, 751, 936, 1138, 1657, 2045, 2484
VpaK11BI	GGWCC	2 cut(s)	1799, 3105
VspI	ATTAAT	2 cut(s)	1835, 2322
XapI	RAATTY	3 cut(s)	918, 1357, 3129
XceI	RCATGY	4 cut(s)	1450, 1616, 2402, 2473
XmnI	GAANNNNTTC	4 cut(s)	537, 1313, 1325, 1425
XspI	CTAG	4 cut(s)	195, 858, 1485, 1946
ZraI	GACGTC	1 cut(s)	190
ZrmI	AGTACT	1 cut(s)	2336

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.