Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0013983

Physical Location & Seq

Genomic Coordinates Forward (+)

58388200 .. 58397644

Gene Structure 9445 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ26624

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3180 bp

ATGGCCTCTACTACTACTACTAGTCTCCAAATCCCCACTTTCCTCTCCCTCAAACCACTCCCTCAACGCCCCTCCTTCGCCTTCTCCAACCACCCCACCTCCTTCTCTTCCTTCTCCACCTCGTCCTCCTCCCGCCCTCCACGCCGTCGTCTCACTCTCTCTCCCGCCCCCATTGCCGCCTCTCTCAAGGAAAATCTGGGCCGGATTGGAAAGACTTGGAGCGACGTGACTAGCTTGAACAGCTGGGTTGTTCGAGACTACTACCGCCTTGTCAATTCTGTCAACTCTTTTGAGCCACAGCTACAGAGGCTCACTGATGACCAGCTTACTGCTAAAACTGCCGAGTTTCGGCGAAGATTGGGACAAGGAGAGACACTTGCGGATATTCAAGCCGAGGCTTTTGCTGTTGTTCGTGAAGCTGCGAAACGGAAGCTTGGTATGCGTCATTTTGATGTTCAGATTATTGGTGGGGCGGTGCTTCATGATGGGTCCATTGCAGAAATGAAGACCGGAGAAGGGAAAACGTTGGTTTCCACATTAGCAGCATATCTTAATGCCCTGACTGGCGAAGGTGTTCATGTGGTAACCGTAAATGATTACCTTGCTCAAAGGGATGCTGAGTGGATGGGTCGTGTTCATCGCTTCCTAGGTCTTTCAGTGGGTCTTATTCAGAGAGGGATGACAGCTGACAAGAGGAGGTCGAACTATAGATGTGACATAACATACACCAATAATTCAGAACTTGGGTTTGACTATCTACGAGACAATCTTGCTGGAAACAGTGGAGAACTTGTTATGAGATCGCCAAAGCCATTTCATTTTGCAATAGTTGACGAAGTTGACTCAGTTCTTATTGATGAAGGAAGAAATCCATTGCTAATTAGTGGCGAGGCTAGTAAAGATGCTGCTCGCTATCCTGTTGCTGCTAAAGTGGCTGAGTTGCTTGTGCGAGGAATTCATTACAATGTAGAACTTAAAGATTACTCAGTTGAGTTGACTGAAGAAGGAATAGCTCTTGCTGAGATGGCTCTTGAAACAAATGACTTATGGGATGAAAATGATCCCTGGGCCAGATTTGTGATGAATGCGTTGAAAGCTAAAGAGTTCTATCGGAAGGATGTGCAATATATTGTCAGAAATGGGAAGGCTCTCATAATCAATGAGCTCACTGGCAGAGTCGAAGATAAGAGAAGGTGGTCTGAGGGTATTCATCAAGCTGTGGAGGGAAAAGAAGGTTTGAAGATTCAGGCTGATTCGGTTGTTATCGCACAAATCACATATCAATCTCTGTTCAAGCTCTACCCAAAGCTATCAGGGATGACTGGCACTGCAAAAACTGAAGAGAAAGAGTTCTTGAAAATGTTCCAAACACCAGTTATTGAAGTACCAACAAATTTGCCAAACATTCGTAATGATTTACCTATTCAAGCTTTTGCGACTGCTCAGGGGAAATGGGAATATGTTCGTCAAGAAGTGGAATACATGTTCAGGCAGGGGCGTCCTGTTTTGGTTGGCACCACCAGTGTTGAGTATTCTGAACACCTGTCTGATCTGTTGAGGGAACATAACATCCCACACAATGTTCTAAATGCACGGCCCAAGTATGCTGCAAGGGAGGCTGAAATTGTTGCCCAAGCTGGACGAAAACATGCCATTACTCTTTCTACTAATATGGCTGGCAGAGGCACTGACATAATCCTAGGGGGGAATCCTAAAATGCTTGCAAAAGAAATCATAGAAGACAGTTTGATTTCATTCCTGACACGAGAAGCTCCTAACATTGACATAGATGGAGAGGCAATTTCACAAAAGGTATTGTCAAAGATTAAGGTCGGTCCATCATCATTAGCTTTGCTGGCTAAGACAGCGTTAATGGCTAAATATGTTGGCAAAAATGAAGGGAAGAGCTGGACATATAAGGAGGCAAAATCCATGATATCAGAGTCTGTGGAAATGAGTCAATCAAGAGATTTGAAAGAGCTAGAGAGGCTTGTTGATGAGCAGTCTGAGATATACCCTCTTGGCCCTACAATAGCACTTGCTTATCTGTCAGTTTTGAAGGATTGCGAGGTACACTGTTTCAAAGAAGGGTCTGAAGTGAAAAGACTTGGGGGACTTCATGTGATTGGAACATCTTTGCACGAGTCTCGTAGAATAGATAACCAGCTTCGTGGCAGAGCAGGAAGGCAAGGTGATCCAGGATCAACTCGATTTATGGTGAGCTTGCAGGATGAGATGTTTCAAAAGTTTAATTTTGATACTGAGTGGGCTGTGAGACTTATTTCAAAAATTACTGATGATGAGGATGTACCAATTGAAGGTGATGCCATTGTGAGACAGCTCCTGTCCCTGCAAATTAATGCTGAAAAGTACTTTTTTGGCATAAGAAAGAGCCTGGTTGAGTTTGATGAAGTATTAGAGGTGCAGAGAAAGCATGTTTATGAACTTCGGCAGTCAATTTTAACTGGTGATAATGAAAGTTGTTCCCAGCATGTATTCCAGTACATGCAAGCAGTGGTAGATGAAATTGTCTTTAAAAATGTTCACGCCCTCAAGCATCCAAGAAAATGGAGTTTAAATAAGCTTTTGAAGGAGTTTATGGCAATTGCTGGGAAACTATTGGATGATTCATTTGCAGGAATTACTGAGGAAGCTTTGTTAGAATCTCTTGCGCAGTCACATGAACTGAACTTTAAAGATATTGATGACATTCACCTTCCAAACTTGCCAAAACCTCCAAATGCCTTCAGAGGTATCCGCAAGAAGAACTCTTCATTGAAACGTTGGCTTGCTATATGCTCGGATGATTTGACCAAGAATGCAAAGTACCATGCTACTACTAACCTCCTCCGCAAGTACCTTGGAGACTATTTGATTGCTTCATATTTGGATGTTGTTCAAGACTCTGGTTATGATGATACATATGTGAAGGAAGTTGAAAGGGCAGTTATTGTGAAAACCCTTGATTGTTTCTGGAGGGATCACCTTGTAAACATGAACAGGCTAAGTTCAGCGGTGAATGTAAGGAGTTTTGGGCACAGGAATCCTCTTGAAGAATACAAGATTGATGGTTGTCGTTTTTTCATATCTATGCTTAGTGCAACACGAAGGTTGACTGTAGAGTCACTTTTGCAATATTGGTCCTCCCCAATGGAGTCTCAAGAAATTTTTGTATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0006996 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0009657 GO:0009658 GO:0009987 GO:0010109 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019222 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0031323 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW WD40 zf-RING_UBOX

Protein Analysis

1059

Amino Acids

119.5

Weight (kDa)

6.7

Isoelectric Point (pI)

38.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	82 - 451	1.8e-116	SecA DEAD-like domain
SecA_PP_bind	PF01043	302 - 408	5.3e-29	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	467 - 754	9.3e-66	SecA P-loop domain
SecA_SW	PF07516	771 - 1045	2.7e-42	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21650 AT1G21650 AT1G21650
fragaria_vesca	FvH4_2g27750
malus_domestica	MD00G1100600.v1.1
prunus_persica	Prupe.1G490400_v2.0.a1
pyrus_communis	pycom15g30570
rosa_chinensis	RchiOBHm_Chr6g0296621
rosa_laevigata	RLG00000011676
rosa_multiflora	Rmu_sc0001741.1_g000019
rosa_roxburghii	Rroxscaffold_7G00171330 Rroxscaffold_7G00178770
rosa_rugosa	Rorug06G0263700
rosa_samantha	Rh6AG375100 Rh6CG388700
rosa_wichuraiana	Rw6G032720 Rw6G032810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3121
Acc16I	TGCGCA	1 cut(s)	2673
AccB1I	GGYRCC	1 cut(s)	1514
AciI	CCGC	9 cut(s)	133, 165, 177, 265, 380, 473, 2758, 2851, 3014
AclI	AACGTT	2 cut(s)	524, 2782
AclWI	GGATC	4 cut(s)	1055, 2189, 2209, 2988
AcsI	RAATTY	3 cut(s)	954, 1393, 3165
AcuI	CTGAAG	4 cut(s)	1020, 1359, 2115, 2731
AcyI	GRCGYC	1 cut(s)	1498
AfaI	GTAC	7 cut(s)	1386, 2073, 2310, 2372, 2504, 2828, 2858
AfiI	CCNNNNNNNGG	3 cut(s)	348, 516, 2318
AflIII	ACRYGT	1 cut(s)	1482
AhlI	ACTAGT	1 cut(s)	20
AjiI	CACGTC	1 cut(s)	226
AjnI	CCWGG	3 cut(s)	1064, 2197, 2394
AloI	GAACNNNNNNTCC	4 cut(s)	1469, 1501, 1554, 1586
Alw21I	GWGCWC	1 cut(s)	1167
AlwI	GGATC	4 cut(s)	1055, 2189, 2209, 2988
AlwNI	CAGNNNCTG	2 cut(s)	1946, 2344
AoxI	GGCC	5 cut(s)	3, 199, 1068, 1595, 2023
ApeKI	GCWGC	5 cut(s)	419, 542, 905, 923, 1607
ApoI	RAATTY	3 cut(s)	954, 1393, 3165
AseI	ATTAAT	1 cut(s)	2358
Asp700I	GAANNNNTTC	4 cut(s)	573, 1349, 1361, 1461
AspA2I	CCTAGG	2 cut(s)	646, 1699
AspLEI	GCGC	1 cut(s)	2674
AspS9I	GGNCC	7 cut(s)	199, 489, 1068, 1596, 1835, 2024, 3141
AsuHPI	GGTGA	7 cut(s)	2204, 2230, 2333, 2480, 2705, 2975, 3028
AvaII	GGWCC	3 cut(s)	489, 1835, 3141
AvrII	CCTAGG	2 cut(s)	646, 1699
BaeGI	GKGCMC	1 cut(s)	3039
BaeI	ACNNNNGTAYC	2 cut(s)	2910, 2943
BanI	GGYRCC	1 cut(s)	1514
BanII	GRGCYC	1 cut(s)	1167
BauI	CACGAG	2 cut(s)	1764, 2141
BbsI	GAAGAC	2 cut(s)	512, 1746
Bbv12I	GWGCWC	1 cut(s)	1167
BbvI	GCAGC	5 cut(s)	406, 554, 892, 910, 1594
BccI	CCATC	6 cut(s)	479, 619, 1018, 1784, 1846, 3062
BceAI	ACGGC	2 cut(s)	129, 1610
BcgI	CGANNNNNNTGC	4 cut(s)	383, 417, 2129, 2163
BciT130I	CCWGG	3 cut(s)	1066, 2199, 2396
BciVI	GTATCC	1 cut(s)	2765
BcuI	ACTAGT	1 cut(s)	20
BfaI	CTAG	6 cut(s)	21, 231, 647, 894, 1700, 1982
BfmI	CTRYAG	3 cut(s)	302, 706, 3117
BfuI	GTATCC	1 cut(s)	2765
BisI	GCNGC	6 cut(s)	177, 420, 543, 906, 924, 1608
BlnI	CCTAGG	2 cut(s)	646, 1699
BlsI	GCNGC	6 cut(s)	178, 421, 544, 907, 925, 1609
BmcAI	AGTACT	1 cut(s)	2372
Bme1390I	CCNGG	3 cut(s)	1066, 2199, 2396
Bme18I	GGWCC	3 cut(s)	489, 1835, 3141
BmgBI	CACGTC	1 cut(s)	226
BmgT120I	GGNCC	7 cut(s)	199, 489, 1068, 1596, 1835, 2024, 3141
BmiI	GGNNCC	2 cut(s)	490, 1516
BmrFI	CCNGG	3 cut(s)	1066, 2199, 2396
BmsI	GCATC	4 cut(s)	604, 892, 2314, 2566
BpiI	GAAGAC	2 cut(s)	512, 1746
BpmI	CTGGAG	1 cut(s)	2995
Bpu10I	CCTNAGC	1 cut(s)	1443
BpuEI	CTTGAG	3 cut(s)	170, 2537, 3144
BsaHI	GRCGYC	1 cut(s)	1498
BsaJI	CCNNGG	6 cut(s)	393, 646, 1064, 1065, 1699, 2860
BsaWI	WCCGGW	1 cut(s)	509
BsaXI	ACNNNNNCTCC	6 cut(s)	83, 113, 145, 175, 777, 807
Bsc4I	CCNNNNNNNGG	3 cut(s)	348, 516, 2318
Bse1I	ACTGG	7 cut(s)	568, 1174, 1327, 1373, 1521, 2470, 2500
Bse3DI	GCAATG	3 cut(s)	171, 492, 872
BseBI	CCWGG	3 cut(s)	1066, 2199, 2396
BseDI	CCNNGG	6 cut(s)	393, 646, 1064, 1065, 1699, 2860
BseLI	CCNNNNNNNGG	3 cut(s)	348, 516, 2318
BseMI	GCAATG	3 cut(s)	171, 492, 872
BseNI	ACTGG	7 cut(s)	568, 1174, 1327, 1373, 1521, 2470, 2500
BseRI	GAGGAG	3 cut(s)	118, 709, 2837
BseSI	GKGCMC	1 cut(s)	3039
BseXI	GCAGC	5 cut(s)	406, 554, 892, 910, 1594
BseYI	CCCAGC	3 cut(s)	243, 2487, 2609
BsgI	GTGCAG	1 cut(s)	2444
BshFI	GGCC	5 cut(s)	5, 201, 1070, 1597, 2025
BshNI	GGYRCC	1 cut(s)	1514
BsiHKAI	GWGCWC	1 cut(s)	1167
BsiSI	CCGG	2 cut(s)	202, 510
BslFI	GGGAC	3 cut(s)	375, 2127, 2332
BslI	CCNNNNNNNGG	3 cut(s)	348, 516, 2318
BsmBI	CGTCTC	1 cut(s)	155
BsmFI	GGGAC	3 cut(s)	375, 2127, 2332
BsmI	GAATGC	2 cut(s)	1090, 2824
BsnI	GGCC	5 cut(s)	5, 201, 1070, 1597, 2025
Bsp1286I	GDGCHC	2 cut(s)	1167, 3039
Bsp143I	GATC	6 cut(s)	800, 1060, 1549, 2194, 2201, 2980
BspACI	CCGC	9 cut(s)	133, 165, 177, 265, 380, 473, 2758, 2851, 3014
BspANI	GGCC	5 cut(s)	5, 201, 1070, 1597, 2025
BspHI	TCATGA	2 cut(s)	481, 3176
BspLI	GGNNCC	2 cut(s)	490, 1516
BspPI	GGATC	4 cut(s)	1055, 2189, 2209, 2988
BspQI	GCTCTTC	1 cut(s)	1898
BspT107I	GGYRCC	1 cut(s)	1514
BsrDI	GCAATG	3 cut(s)	171, 492, 872
BsrI	ACTGG	7 cut(s)	568, 1174, 1327, 1373, 1521, 2470, 2500
BssECI	CCNNGG	6 cut(s)	393, 646, 1064, 1065, 1699, 2860
BssMI	GATC	6 cut(s)	800, 1060, 1549, 2194, 2201, 2980
BssNI	GRCGYC	1 cut(s)	1498
BssSI	CACGAG	2 cut(s)	1764, 2141
BssT1I	CCWWGG	3 cut(s)	646, 1699, 2860
Bst2BI	CACGAG	2 cut(s)	1764, 2141
Bst2UI	CCWGG	3 cut(s)	1066, 2199, 2396
Bst4CI	ACNGT	5 cut(s)	589, 782, 1745, 2078, 3118
Bst6I	CTCTTC	4 cut(s)	112, 1335, 1898, 2776
BstACI	GRCGYC	1 cut(s)	1498
BstC8I	GCNNGC	7 cut(s)	910, 1678, 1722, 1857, 2225, 2511, 2790
BstEII	GGTNACC	1 cut(s)	583
BstHHI	GCGC	1 cut(s)	2674
BstKTI	GATC	6 cut(s)	803, 1063, 1552, 2197, 2204, 2983
BstMBI	GATC	6 cut(s)	800, 1060, 1549, 2194, 2201, 2980
BstNI	CCWGG	3 cut(s)	1066, 2199, 2396
BstNSI	RCATGY	5 cut(s)	1486, 1652, 2438, 2495, 2509
BstPI	GGTNACC	1 cut(s)	583
BstSCI	CCNGG	3 cut(s)	1064, 2197, 2394
BstSFI	CTRYAG	3 cut(s)	302, 706, 3117
BstSLI	GKGCMC	1 cut(s)	3039
BstV1I	GCAGC	5 cut(s)	406, 554, 892, 910, 1594
BstV2I	GAAGAC	2 cut(s)	512, 1746
BstXI	CCANNNNNNTGG	2 cut(s)	2171, 2568
BsuI	GTATCC	1 cut(s)	2765
BsuRI	GGCC	5 cut(s)	5, 201, 1070, 1597, 2025
BtgZI	GCGATG	1 cut(s)	623
BtrI	CACGTC	1 cut(s)	226
BtsI	GCAGTG	2 cut(s)	1326, 2520
BtsIMutI	CAGTG	9 cut(s)	312, 663, 787, 1167, 1326, 1528, 1686, 2074, 2520
Cac8I	GCNNGC	7 cut(s)	910, 1678, 1722, 1857, 2225, 2511, 2790
CaiI	CAGNNNCTG	2 cut(s)	1946, 2344
CciI	TCATGA	2 cut(s)	481, 3176
CfoI	GCGC	1 cut(s)	2674
Cfr13I	GGNCC	7 cut(s)	199, 489, 1068, 1596, 1835, 2024, 3141
CseI	GACGC	2 cut(s)	431, 1487
Csp6I	GTAC	7 cut(s)	1385, 2072, 2309, 2371, 2503, 2827, 2857
CspCI	CAANNNNNGTGG	2 cut(s)	1507, 1542
CviQI	GTAC	7 cut(s)	1385, 2072, 2309, 2371, 2503, 2827, 2857
DpnI	GATC	6 cut(s)	802, 1062, 1551, 2196, 2203, 2982
DpnII	GATC	6 cut(s)	800, 1060, 1549, 2194, 2201, 2980
DraI	TTTAAA	3 cut(s)	2536, 2577, 2695
DrdI	GACNNNNNNGTC	1 cut(s)	3121
DseDI	GACNNNNNNGTC	1 cut(s)	3121
Eam1104I	CTCTTC	4 cut(s)	112, 1335, 1898, 2776
EarI	CTCTTC	4 cut(s)	112, 1335, 1898, 2776
Ecl136II	GAGCTC	1 cut(s)	1165
Eco130I	CCWWGG	3 cut(s)	646, 1699, 2860
Eco24I	GRGCYC	1 cut(s)	1167
Eco32I	GATATC	1 cut(s)	1938
Eco47I	GGWCC	3 cut(s)	489, 1835, 3141
Eco53kI	GAGCTC	1 cut(s)	1165
Eco57I	CTGAAG	4 cut(s)	1020, 1359, 2115, 2731
Eco91I	GGTNACC	1 cut(s)	583
EcoICRI	GAGCTC	1 cut(s)	1165
EcoO65I	GGTNACC	1 cut(s)	583
EcoRI	GAATTC	1 cut(s)	954
EcoRII	CCWGG	3 cut(s)	1064, 2197, 2394
EcoRV	GATATC	1 cut(s)	1938
EcoT14I	CCWWGG	3 cut(s)	646, 1699, 2860
EcoT38I	GRGCYC	1 cut(s)	1167
ErhI	CCWWGG	3 cut(s)	646, 1699, 2860
Esp3I	CGTCTC	1 cut(s)	155
FaqI	GGGAC	3 cut(s)	375, 2127, 2332
FauI	CCCGC	2 cut(s)	140, 172
FauNDI	CATATG	1 cut(s)	2923
Fnu4HI	GCNGC	6 cut(s)	177, 420, 543, 906, 924, 1608
FriOI	GRGCYC	1 cut(s)	1167
Fsp4HI	GCNGC	6 cut(s)	177, 420, 543, 906, 924, 1608
FspBI	CTAG	6 cut(s)	21, 231, 647, 894, 1700, 1982
FspI	TGCGCA	1 cut(s)	2673
GlaI	GCGC	1 cut(s)	2673
GluI	GCNGC	6 cut(s)	177, 420, 543, 906, 924, 1608
GsaI	CCCAGC	3 cut(s)	247, 2491, 2613
GsuI	CTGGAG	1 cut(s)	2995
HaeIII	GGCC	5 cut(s)	5, 201, 1070, 1597, 2025
HapII	CCGG	2 cut(s)	202, 510
HgaI	GACGC	2 cut(s)	431, 1487
HhaI	GCGC	1 cut(s)	2674
Hin1I	GRCGYC	1 cut(s)	1498
Hin6I	GCGC	1 cut(s)	2672
HinP1I	GCGC	1 cut(s)	2672
HincII	GTYRAC	5 cut(s)	283, 832, 841, 996, 3114
HindII	GTYRAC	5 cut(s)	283, 832, 841, 996, 3114
HindIII	AAGCTT	4 cut(s)	431, 1428, 2582, 2652
HpaII	CCGG	2 cut(s)	202, 510
HphI	GGTGA	7 cut(s)	2204, 2230, 2333, 2480, 2705, 2975, 3028
Hpy166II	GTNNAC	8 cut(s)	283, 832, 841, 996, 2074, 2545, 2992, 3114
Hpy8I	GTNNAC	8 cut(s)	283, 832, 841, 996, 2074, 2545, 2992, 3114
Hpy99I	CGWCG	2 cut(s)	150, 227
HpyCH4III	ACNGT	5 cut(s)	589, 782, 1745, 2078, 3118
HpyCH4IV	ACGT	3 cut(s)	225, 524, 2782
HpySE526I	ACGT	3 cut(s)	225, 524, 2782
Hsp92I	GRCGYC	1 cut(s)	1498
HspAI	GCGC	1 cut(s)	2672
Kzo9I	GATC	6 cut(s)	800, 1060, 1549, 2194, 2201, 2980
LguI	GCTCTTC	1 cut(s)	1898
LmnI	GCTCC	3 cut(s)	219, 1777, 2346
Lsp1109I	GCAGC	5 cut(s)	406, 554, 892, 910, 1594
LweI	GCATC	4 cut(s)	604, 892, 2314, 2566
MaeI	CTAG	6 cut(s)	21, 231, 647, 894, 1700, 1982
MaeII	ACGT	3 cut(s)	225, 524, 2782
MaeIII	GTNAC	5 cut(s)	226, 583, 713, 2676, 3123
MalI	GATC	6 cut(s)	802, 1062, 1551, 2196, 2203, 2982
MboI	GATC	6 cut(s)	800, 1060, 1549, 2194, 2201, 2980
MfeI	CAATTG	2 cut(s)	2313, 2604
MhlI	GDGCHC	2 cut(s)	1167, 3039
MlyI	GAGTC	8 cut(s)	836, 1185, 1952, 1966, 2153, 2897, 3131, 3164
MmeI	TCCRAC	1 cut(s)	111
MroXI	GAANNNNTTC	4 cut(s)	573, 1349, 1361, 1461
MslI	CAYNNNNRTG	4 cut(s)	450, 963, 2439, 3089
MspA1I	CMGCKG	3 cut(s)	243, 686, 3014
MspI	CCGG	2 cut(s)	202, 510
MspR9I	CCNGG	3 cut(s)	1066, 2199, 2396
MunI	CAATTG	2 cut(s)	2313, 2604
Mva1269I	GAATGC	2 cut(s)	1090, 2824
MvaI	CCWGG	3 cut(s)	1066, 2199, 2396
NdeI	CATATG	1 cut(s)	2923
NdeII	GATC	6 cut(s)	800, 1060, 1549, 2194, 2201, 2980
NlaIV	GGNNCC	2 cut(s)	490, 1516
NmeAIII	GCCGAG	2 cut(s)	367, 418
NmuCI	GTSAC	4 cut(s)	226, 713, 2676, 3123
NsbI	TGCGCA	1 cut(s)	2673
NspI	RCATGY	5 cut(s)	1486, 1652, 2438, 2495, 2509
PagI	TCATGA	2 cut(s)	481, 3176
PasI	CCCWGGG	1 cut(s)	1065
PciI	ACATGT	1 cut(s)	1482
PciSI	GCTCTTC	1 cut(s)	1898
PctI	GAATGC	2 cut(s)	1090, 2824
PdmI	GAANNNNTTC	4 cut(s)	573, 1349, 1361, 1461
PfeI	GAWTC	6 cut(s)	1243, 1253, 1708, 2627, 2663, 3043
PfoI	TCCNGGA	1 cut(s)	2197
PkrI	GCNGC	6 cut(s)	178, 421, 544, 907, 925, 1609
PleI	GAGTC	8 cut(s)	836, 1184, 1951, 1965, 2152, 2897, 3130, 3163
PpsI	GAGTC	8 cut(s)	836, 1184, 1951, 1965, 2152, 2897, 3130, 3163
PscI	ACATGT	1 cut(s)	1482
PshBI	ATTAAT	1 cut(s)	2358
Psp124BI	GAGCTC	1 cut(s)	1167
Psp1406I	AACGTT	2 cut(s)	524, 2782
Psp6I	CCWGG	3 cut(s)	1064, 2197, 2394
PspEI	GGTNACC	1 cut(s)	583
PspFI	CCCAGC	3 cut(s)	243, 2487, 2609
PspGI	CCWGG	3 cut(s)	1064, 2197, 2394
PspN4I	GGNNCC	2 cut(s)	490, 1516
PspPI	GGNCC	7 cut(s)	199, 489, 1068, 1596, 1835, 2024, 3141
PstNI	CAGNNNCTG	2 cut(s)	1946, 2344
PvuII	CAGCTG	2 cut(s)	243, 686
RsaI	GTAC	7 cut(s)	1386, 2073, 2310, 2372, 2504, 2828, 2858
RsaNI	GTAC	7 cut(s)	1385, 2072, 2309, 2371, 2503, 2827, 2857
RseI	CAYNNNNRTG	4 cut(s)	450, 963, 2439, 3089
SacI	GAGCTC	1 cut(s)	1167
SapI	GCTCTTC	1 cut(s)	1898
SatI	GCNGC	6 cut(s)	177, 420, 543, 906, 924, 1608
Sau3AI	GATC	6 cut(s)	800, 1060, 1549, 2194, 2201, 2980
Sau96I	GGNCC	7 cut(s)	199, 489, 1068, 1596, 1835, 2024, 3141
ScaI	AGTACT	1 cut(s)	2372
SchI	GAGTC	8 cut(s)	836, 1185, 1952, 1966, 2153, 2897, 3131, 3164
ScrFI	CCNGG	3 cut(s)	1066, 2199, 2396
SduI	GDGCHC	2 cut(s)	1167, 3039
SfaNI	GCATC	4 cut(s)	604, 892, 2314, 2566
SfcI	CTRYAG	3 cut(s)	302, 706, 3117
SinI	GGWCC	3 cut(s)	489, 1835, 3141
SmiMI	CAYNNNNRTG	4 cut(s)	450, 963, 2439, 3089
SmlI	CTYRAG	3 cut(s)	185, 2552, 3159
SmoI	CTYRAG	3 cut(s)	185, 2552, 3159
SpeI	ACTAGT	1 cut(s)	20
SsiI	CCGC	9 cut(s)	133, 165, 177, 265, 380, 473, 2758, 2851, 3014
SspI	AATATT	1 cut(s)	3137
SspMI	CTAG	6 cut(s)	21, 231, 647, 894, 1700, 1982
SstI	GAGCTC	1 cut(s)	1167
StyD4I	CCNGG	3 cut(s)	1064, 2197, 2394
StyI	CCWWGG	3 cut(s)	646, 1699, 2860
TaaI	ACNGT	5 cut(s)	589, 782, 1745, 2078, 3118
TaiI	ACGT	3 cut(s)	228, 527, 2785
TaqI	TCGA	4 cut(s)	253, 701, 1179, 2209
TaqII	GACCGA	1 cut(s)	1823
TatI	WGTACW	2 cut(s)	2370, 2502
TauI	GCSGC	1 cut(s)	179
TfiI	GAWTC	6 cut(s)	1243, 1253, 1708, 2627, 2663, 3043
TscAI	CASTG	9 cut(s)	319, 663, 787, 1174, 1333, 1528, 1693, 2081, 2520
TseFI	GTSAC	4 cut(s)	226, 713, 2676, 3123
TseI	GCWGC	5 cut(s)	419, 542, 905, 923, 1607
Tsp45I	GTSAC	4 cut(s)	226, 713, 2676, 3123
TspGWI	ACGGA	1 cut(s)	442
TspRI	CASTG	9 cut(s)	319, 663, 787, 1174, 1333, 1528, 1693, 2081, 2520
VpaK11BI	GGWCC	3 cut(s)	489, 1835, 3141
VspI	ATTAAT	1 cut(s)	2358
XapI	RAATTY	3 cut(s)	954, 1393, 3165
XceI	RCATGY	5 cut(s)	1486, 1652, 2438, 2495, 2509
XmaJI	CCTAGG	2 cut(s)	646, 1699
XmnI	GAANNNNTTC	4 cut(s)	573, 1349, 1361, 1461
XspI	CTAG	6 cut(s)	21, 231, 647, 894, 1700, 1982
ZrmI	AGTACT	1 cut(s)	2372

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.