Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0013983

Physical Location & Seq

Genomic Coordinates Forward (+)

18427781 .. 18438889

Gene Structure 11109 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00178770.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3249 bp

ATGTTCCATAGTACAAATCGTACTCCATGTCATAATCTCCACCTAGTTACAGTTACAGTTCCCGCCATGGCCTCTACTACTAGTCTCCAAATCCCCACTTTCCTCTCCCTCAAACCACTCCCTCAACGCCCCACCTTCGCCTTCTCCAACCACCCCACCTCCTTCTCTTCCGTCTCCTCCTCCTCCTCCCGCCCTCCACGCCGTCGTCTCACTCTCTCTCCCGCCCCCATTGCCGCCTCTCTCAAGGAAACTCTGGGCCGGATTGGAAAGACTTGGAACGACGTGACTAGCTTGAACAGCTGGGTTGTTCGAGACTACTACCGCCTTGTCAATTCTGTCAACTCTTTTGAGCCACAGCTACAGAGGCTCACTGATGACCAGCTTACTGCTAAAACTGCCGAGTTTCGGCGAAGATTGGGACAAGGAGAGACACTTGCGGATATTCAAGCTGAGGCTTTTGCTGTTGTTCGTGAAGCTGCGAAACGGAAGCTTGGTATGCGTCATTTCGATGTTCAGATTATTGGTGGGGCGGTGCTTCATGATGGGTCCATTGCAGAAATGAAGACCGGAGAAGGGAAAACGTTGGTTTCCACATTAGCAGCATATCTTAATGCCCTGACTGGCGAAGGTGTTCATGTGGTAACCGTAAATGATTACCTTGCTCAAAGAGATGCTGAGTGGATGGGTCGTGTTCATCGCTTCCTAGGTCTTTCAGTGGGTCTTATTCAGGAACTTGGTTTTGACTATCTACGAGACAATCTTGCCGGAAACAGGGGAGAACTTGTTATGAGATCGCCAAAGCCATTTCATTTTGCAATAGTTGATGAAGTTGACTCTGTTCTTATTGATGAAGGAAGAAATCCATTGCTAATTAGTGGCGAGGCTAGTAAAGATGCTGCACGCTATCCTGTTGCTGCTAAAGTGGCTGAGTTGCTTGTGCGAGGAATTCATTACAATGTGGAACTTAAAGATTACTCAGTTGAGTTGACTGAAGAAGGAATAGCTCTTGCTGAGATGGCTCTTGAAACAAATGACTTATGGGATGAAAATGATCCCTGGGCCAGATTTGTGATGAATGCGTTGAAAGCTAAAGAGTTCTATCGGAAGGATGTGCAATATATTGTCAGAAATGGGAAGGCTCTCATAATCAATGAGCTCACTGGCAGAGTCGAAGATAAGAGAAGGTGGTCTGAGGGTATTCATCAAGCTGTGGAGGGTAAAGAAGGTTTGAAGATTCAGGCTGATTCAGTTGTTATCGCACAAATCACCTATCAATCTCTGTTCAAGCTCTACCCAAAGCTATCAGGGATGACTGGGACTGCAAAAACTGAAGAGAAAGAGTTCTTGAAAATGTTCCAGACACCAGTTATTGAAGTGCCAACAAATTTGCCAAACATTCGTAATGATTTACCTATTCAAGCTTTTGCGACTGCTCAGGGGAAATGGGAATATGTTCGTCAAGAAGTGGAATACATGTTCAGGCAGGGGCGTCCCGTTTTGGTTGGCACCACCAGTGTTGAGTATTCTGAACACCTGTCTGACCTATTGAGGGAACATAACATCCCACACAATGTTCTAAATGCACGGCCGAAGTATGCTGCAAGGGAGGCTGAAATTGTTGCCCAAGCTGGACGAAAACATGCCATTACTCTTTCTACCAATATGGCTGGCAGAGGCACTGACATAATCCTAGGAGGGAATCCTAAAATGCTTGCAAAAGAGATCATAGAAGACAGTTTGATTTCATTCCTGACACGAGAAGCTCCTAACGTTGACATAGATGGAGAGGCAATTTCACAAAAGGTATTGTCAAAGATAAAGGTCGGTCCATCATCATTAGCTTTGCTGGCTAAGACAGCATTAATGGCTAAATATGTTGGCAAAAATGAAGGGAAGAGCTGGACATATAAGGAGGCAAAATCCATGATATCAGAGTCTGTGGAAATGAGTCAATCAAGAGATTTGAAAGAGCTAGAGAGGCTTGTTGATGAACAGTCTGAGATGTACCCTCTTGGCCCTACAATAGCACTTGCTTATCTGTCAGTTTTGAAGGATTGCGAGGTACATTGTTTCAAAGAAGGGTCTGAAGTGAAAAGGCTTGGGGGCCTTCATGTGATTGGAACATCTTTGCACGAGTCTCGTAGAATAGATAACCAGTTTTGGGGTGAATTTGGATTTGATATGCATCCATCCAATTTCAGCTTGGGAGTTGCTGAGAGTGCTCCTCTTTTTCCTTTGAAAATTTTCCTTCGTGGCAGAGCAGGAAGGCAAGGTGATCCAGGATCAACTCGATTTATGGTGAGCTTGCAGGATGAGATGTTTCAAAAGTTCAATTTTGATACTGAGTGGGCTGTGAGACTTATTTCAAAGATTACTGATGATGAGGATGTACCAATTGAAGGTGATGCAATTGTGAGACAGCTCCTGTCCCTGCAAATTAATGCTGAAAAGTACTTTTTTGGCATAAGAAAGAGCCTGGTTGAGTTTGATGAAGTATTAGAGGTGCAAAGAAAGCATGTTTATGAACTTAGGCAGTCAATTTTAACTGGTGATAATGAAAGTTGTTCCCAGCATGTATTCCAGTACATGCAAGCAGTGGTAGATGAAATTGTTTTTAAAAATGTTGACGCTCTCAAGCATCCAAGAAACTGGAGTTTGAATAAGCTTTTGAAGGAGTTTATGGCGATTGCTGGGAAACTATTGGATGGAATTACTGAGGAAGCTTTGTTAGAATCTCTTGCGCAATCACATGAACTGAACTCTAAAGATATTGATGACATTCACCTTCCAAACTTGCCAAGACCTCCTAATGCCTTCAGAGGTATCCGCAAGAAGAACTCTTCATTGAAACGTTGGCTTGCTATATGCTCGGATGATTTGACCAAGAATGGAAGGTATCATGCTACTACTAACCTCCTCCGCAAGTACCTTGGAGACTATTTGATTGCTTCGTATTTGGATGTTGTTCAAGACTCTGGTTATGATGATACATATGTGAAGGAAGTTGAAAGAGCAGTTATTGTGAAAACCCTTGATTGTTTCTGGAGGGATCACCTTGTAAACATGAACAGGCTAAGTTCAGCGGTGAATGTAAGGAGTTTTGGGCACAGGAATCCTCTTGAAGAATACAAGATTGATGGTTGTCGATTTTTCATATCTATGCTTAGTGCAACGCGAAGGTTGACTGTAGAGTCACTTTTGCAATATTGGTCCTCCCCAATGGAGTCTCAAGAAATTTTTGTATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0006996 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0009657 GO:0009658 GO:0009987 GO:0010109 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019222 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0031323 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW WD40 zf-RING_UBOX

Protein Analysis

1082

Amino Acids

122.0

Weight (kDa)

6.42

Isoelectric Point (pI)

37.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	100 - 448	1.4e-96	SecA DEAD-like domain
SecA_PP_bind	PF01043	299 - 405	5.5e-29	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	464 - 725	6.1e-49	SecA P-loop domain
P-loop_SecA	PF21090	750 - 781	1.9e-08	SecA P-loop domain
SecA_SW	PF07516	798 - 1068	2.9e-42	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0013983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21650 AT1G21650 AT1G21650
fragaria_vesca	FvH4_2g27750
malus_domestica	MD00G1100600.v1.1
prunus_persica	Prupe.1G490400_v2.0.a1
pyrus_communis	pycom15g30570
rosa_chinensis	RchiOBHm_Chr6g0296621
rosa_laevigata	RLG00000011676
rosa_multiflora	Rmu_sc0001741.1_g000019
rosa_roxburghii	Rroxscaffold_7G00171330 Rroxscaffold_7G00178770
rosa_rugosa	Rorug06G0263700
rosa_samantha	Rh6AG375100 Rh6CG388700
rosa_wichuraiana	Rw6G032720 Rw6G032810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3190
Acc16I	TGCGCA	1 cut(s)	2742
AccB1I	GGYRCC	1 cut(s)	1505
AccII	CGCG	1 cut(s)	3175
AclI	AACGTT	3 cut(s)	581, 1770, 2851
AclWI	GGATC	4 cut(s)	1046, 2270, 2290, 3057
AcoI	YGGCCR	1 cut(s)	1586
AcsI	RAATTY	5 cut(s)	945, 1384, 2168, 2241, 3234
AcuI	CTGAAG	4 cut(s)	1011, 1350, 2106, 2800
AcyI	GRCGYC	1 cut(s)	1489
AfaI	GTAC	8 cut(s)	13, 22, 2006, 2064, 2391, 2453, 2585, 2927
AfiI	CCNNNNNNNGG	6 cut(s)	405, 573, 771, 1549, 2161, 2399
AflIII	ACRYGT	1 cut(s)	1473
AhlI	ACTAGT	1 cut(s)	80
AjiI	CACGTC	1 cut(s)	283
AjnI	CCWGG	3 cut(s)	1055, 2278, 2475
AloI	GAACNNNNNNTCC	4 cut(s)	1460, 1492, 1545, 1577
Alw21I	GWGCWC	2 cut(s)	1158, 2224
AlwI	GGATC	4 cut(s)	1046, 2270, 2290, 3057
AlwNI	CAGNNNCTG	2 cut(s)	1937, 2425
AoxI	GGCC	6 cut(s)	69, 256, 1059, 1586, 2014, 2104
ApeKI	GCWGC	5 cut(s)	476, 599, 896, 914, 1598
ApoI	RAATTY	5 cut(s)	945, 1384, 2168, 2241, 3234
AseI	ATTAAT	2 cut(s)	1862, 2439
Asp700I	GAANNNNTTC	5 cut(s)	630, 1340, 1352, 1452, 2243
AspA2I	CCTAGG	2 cut(s)	703, 1690
AspLEI	GCGC	1 cut(s)	2743
AspS9I	GGNCC	7 cut(s)	256, 546, 1059, 1826, 2015, 2104, 3210
AsuHPI	GGTGA	9 cut(s)	1258, 2177, 2285, 2311, 2414, 2561, 2774, 3044, 3097
AvaII	GGWCC	3 cut(s)	546, 1826, 3210
AvrII	CCTAGG	2 cut(s)	703, 1690
BaeGI	GKGCMC	1 cut(s)	3108
BaeI	ACNNNNGTAYC	2 cut(s)	2979, 3012
BanI	GGYRCC	1 cut(s)	1505
BanII	GRGCYC	1 cut(s)	1158
BauI	CACGAG	2 cut(s)	1755, 2132
BbsI	GAAGAC	2 cut(s)	569, 1737
Bbv12I	GWGCWC	2 cut(s)	1158, 2224
BbvCI	CCTCAGC	1 cut(s)	450
BbvI	GCAGC	5 cut(s)	463, 611, 883, 901, 1585
BccI	CCATC	8 cut(s)	536, 676, 1009, 1775, 1837, 2197, 2699, 3131
BceAI	ACGGC	2 cut(s)	186, 1601
BcgI	CGANNNNNNTGC	2 cut(s)	2120, 2154
BciT130I	CCWGG	3 cut(s)	1057, 2280, 2477
BciVI	GTATCC	1 cut(s)	2834
BcuI	ACTAGT	1 cut(s)	80
BfaI	CTAG	7 cut(s)	44, 81, 288, 704, 885, 1691, 1973
BfmI	CTRYAG	2 cut(s)	359, 3186
BfuI	GTATCC	1 cut(s)	2834
BisI	GCNGC	6 cut(s)	234, 477, 600, 897, 915, 1599
BlnI	CCTAGG	2 cut(s)	703, 1690
BlsI	GCNGC	6 cut(s)	235, 478, 601, 898, 916, 1600
BmcAI	AGTACT	1 cut(s)	2453
Bme1390I	CCNGG	3 cut(s)	1057, 2280, 2477
Bme18I	GGWCC	3 cut(s)	546, 1826, 3210
BmgBI	CACGTC	1 cut(s)	283
BmgT120I	GGNCC	7 cut(s)	256, 546, 1059, 1826, 2015, 2104, 3210
BmiI	GGNNCC	3 cut(s)	547, 1507, 2105
BmrFI	CCNGG	3 cut(s)	1057, 2280, 2477
BmrI	ACTGGG	1 cut(s)	1323
BmsI	GCATC	5 cut(s)	661, 883, 2194, 2395, 2647
BmuI	ACTGGG	1 cut(s)	1323
BpiI	GAAGAC	2 cut(s)	569, 1737
BplI	GAGNNNNNCTC	2 cut(s)	2209, 2241
BpmI	CTGGAG	2 cut(s)	2671, 3064
Bpu10I	CCTNAGC	2 cut(s)	450, 1434
BpuEI	CTTGAG	3 cut(s)	227, 2618, 3213
BsaBI	GATNNNNATC	1 cut(s)	2184
BsaHI	GRCGYC	1 cut(s)	1489
BsaJI	CCNNGG	6 cut(s)	66, 703, 1055, 1056, 1690, 2929
BsaWI	WCCGGW	1 cut(s)	566
BsaXI	ACNNNNNCTCC	6 cut(s)	143, 173, 202, 232, 768, 798
Bsc4I	CCNNNNNNNGG	6 cut(s)	405, 573, 771, 1549, 2161, 2399
Bse1I	ACTGG	9 cut(s)	625, 1165, 1318, 1364, 1512, 2155, 2551, 2581, 2654
Bse3DI	GCAATG	3 cut(s)	228, 549, 863
Bse8I	GATNNNNATC	1 cut(s)	2184
BseBI	CCWGG	3 cut(s)	1057, 2280, 2477
BseDI	CCNNGG	6 cut(s)	66, 703, 1055, 1056, 1690, 2929
BseJI	GATNNNNATC	1 cut(s)	2184
BseLI	CCNNNNNNNGG	6 cut(s)	405, 573, 771, 1549, 2161, 2399
BseMI	GCAATG	3 cut(s)	228, 549, 863
BseNI	ACTGG	9 cut(s)	625, 1165, 1318, 1364, 1512, 2155, 2551, 2581, 2654
BseRI	GAGGAG	6 cut(s)	166, 169, 172, 175, 2214, 2906
BseSI	GKGCMC	1 cut(s)	3108
BseX3I	CGGCCG	1 cut(s)	1586
BseXI	GCAGC	5 cut(s)	463, 611, 883, 901, 1585
BseYI	CCCAGC	3 cut(s)	300, 2568, 2690
BsgI	GTGCAG	1 cut(s)	882
Bsh1236I	CGCG	1 cut(s)	3175
Bsh1285I	CGRYCG	1 cut(s)	1589
BshFI	GGCC	6 cut(s)	71, 258, 1061, 1588, 2016, 2106
BshNI	GGYRCC	1 cut(s)	1505
BsiEI	CGRYCG	1 cut(s)	1589
BsiHKAI	GWGCWC	2 cut(s)	1158, 2224
BsiSI	CCGG	3 cut(s)	259, 567, 765
BslFI	GGGAC	4 cut(s)	432, 1330, 1476, 2413
BslI	CCNNNNNNNGG	6 cut(s)	405, 573, 771, 1549, 2161, 2399
BsmBI	CGTCTC	2 cut(s)	178, 212
BsmFI	GGGAC	4 cut(s)	432, 1330, 1476, 2413
BsmI	GAATGC	1 cut(s)	1081
BsnI	GGCC	6 cut(s)	71, 258, 1061, 1588, 2016, 2106
Bsp1286I	GDGCHC	3 cut(s)	1158, 2224, 3108
Bsp143I	GATC	6 cut(s)	791, 1051, 1722, 2275, 2282, 3049
Bsp19I	CCATGG	1 cut(s)	66
BspANI	GGCC	6 cut(s)	71, 258, 1061, 1588, 2016, 2106
BspFNI	CGCG	1 cut(s)	3175
BspHI	TCATGA	2 cut(s)	538, 3245
BspLI	GGNNCC	3 cut(s)	547, 1507, 2105
BspPI	GGATC	4 cut(s)	1046, 2270, 2290, 3057
BspQI	GCTCTTC	1 cut(s)	1889
BspT107I	GGYRCC	1 cut(s)	1505
BsrDI	GCAATG	3 cut(s)	228, 549, 863
BsrI	ACTGG	9 cut(s)	625, 1165, 1318, 1364, 1512, 2155, 2551, 2581, 2654
BssECI	CCNNGG	6 cut(s)	66, 703, 1055, 1056, 1690, 2929
BssMI	GATC	6 cut(s)	791, 1051, 1722, 2275, 2282, 3049
BssNI	GRCGYC	1 cut(s)	1489
BssSI	CACGAG	2 cut(s)	1755, 2132
BssT1I	CCWWGG	4 cut(s)	66, 703, 1690, 2929
Bst2BI	CACGAG	2 cut(s)	1755, 2132
Bst2UI	CCWGG	3 cut(s)	1057, 2280, 2477
Bst4CI	ACNGT	6 cut(s)	52, 58, 646, 1736, 1995, 3187
Bst6I	CTCTTC	4 cut(s)	172, 1326, 1889, 2845
BstACI	GRCGYC	1 cut(s)	1489
BstC8I	GCNNGC	7 cut(s)	901, 1669, 1713, 1848, 2306, 2592, 2859
BstDSI	CCRYGG	1 cut(s)	66
BstEII	GGTNACC	1 cut(s)	640
BstFNI	CGCG	1 cut(s)	3175
BstHHI	GCGC	1 cut(s)	2743
BstKTI	GATC	6 cut(s)	794, 1054, 1725, 2278, 2285, 3052
BstMBI	GATC	6 cut(s)	791, 1051, 1722, 2275, 2282, 3049
BstMCI	CGRYCG	1 cut(s)	1589
BstNI	CCWGG	3 cut(s)	1057, 2280, 2477
BstNSI	RCATGY	5 cut(s)	1477, 1643, 2519, 2576, 2590
BstPI	GGTNACC	1 cut(s)	640
BstSCI	CCNGG	3 cut(s)	1055, 2278, 2475
BstSFI	CTRYAG	2 cut(s)	359, 3186
BstSLI	GKGCMC	1 cut(s)	3108
BstUI	CGCG	1 cut(s)	3175
BstV1I	GCAGC	5 cut(s)	463, 611, 883, 901, 1585
BstV2I	GAAGAC	2 cut(s)	569, 1737
BstXI	CCANNNNNNTGG	1 cut(s)	2649
BstZI	CGGCCG	1 cut(s)	1586
BsuI	GTATCC	1 cut(s)	2834
BsuRI	GGCC	6 cut(s)	71, 258, 1061, 1588, 2016, 2106
BtgI	CCRYGG	1 cut(s)	66
BtgZI	GCGATG	1 cut(s)	680
BtrI	CACGTC	1 cut(s)	283
BtsI	GCAGTG	1 cut(s)	2601
BtsIMutI	CAGTG	6 cut(s)	369, 720, 1158, 1519, 1677, 2601
Cac8I	GCNNGC	7 cut(s)	901, 1669, 1713, 1848, 2306, 2592, 2859
CaiI	CAGNNNCTG	2 cut(s)	1937, 2425
CciI	TCATGA	2 cut(s)	538, 3245
CfoI	GCGC	1 cut(s)	2743
Cfr13I	GGNCC	7 cut(s)	256, 546, 1059, 1826, 2015, 2104, 3210
CseI	GACGC	3 cut(s)	488, 1478, 2636
Csp6I	GTAC	8 cut(s)	12, 21, 2005, 2063, 2390, 2452, 2584, 2926
CspCI	CAANNNNNGTGG	2 cut(s)	1498, 1533
CviQI	GTAC	8 cut(s)	12, 21, 2005, 2063, 2390, 2452, 2584, 2926
DpnI	GATC	6 cut(s)	793, 1053, 1724, 2277, 2284, 3051
DpnII	GATC	6 cut(s)	791, 1051, 1722, 2275, 2282, 3049
DraI	TTTAAA	1 cut(s)	2617
DrdI	GACNNNNNNGTC	1 cut(s)	3190
DseDI	GACNNNNNNGTC	1 cut(s)	3190
EaeI	YGGCCR	1 cut(s)	1586
EagI	CGGCCG	1 cut(s)	1586
Eam1104I	CTCTTC	4 cut(s)	172, 1326, 1889, 2845
EarI	CTCTTC	4 cut(s)	172, 1326, 1889, 2845
Ecl136II	GAGCTC	1 cut(s)	1156
EclXI	CGGCCG	1 cut(s)	1586
Eco130I	CCWWGG	4 cut(s)	66, 703, 1690, 2929
Eco24I	GRGCYC	1 cut(s)	1158
Eco32I	GATATC	1 cut(s)	1929
Eco47I	GGWCC	3 cut(s)	546, 1826, 3210
Eco52I	CGGCCG	1 cut(s)	1586
Eco53kI	GAGCTC	1 cut(s)	1156
Eco57I	CTGAAG	4 cut(s)	1011, 1350, 2106, 2800
Eco91I	GGTNACC	1 cut(s)	640
EcoICRI	GAGCTC	1 cut(s)	1156
EcoO109I	RGGNCCY	1 cut(s)	2104
EcoO65I	GGTNACC	1 cut(s)	640
EcoRI	GAATTC	1 cut(s)	945
EcoRII	CCWGG	3 cut(s)	1055, 2278, 2475
EcoRV	GATATC	1 cut(s)	1929
EcoT14I	CCWWGG	4 cut(s)	66, 703, 1690, 2929
EcoT22I	ATGCAT	1 cut(s)	2187
EcoT38I	GRGCYC	1 cut(s)	1158
ErhI	CCWWGG	4 cut(s)	66, 703, 1690, 2929
Esp3I	CGTCTC	2 cut(s)	178, 212
FaqI	GGGAC	4 cut(s)	432, 1330, 1476, 2413
FauI	CCCGC	3 cut(s)	70, 197, 229
FauNDI	CATATG	1 cut(s)	2992
Fnu4HI	GCNGC	6 cut(s)	234, 477, 600, 897, 915, 1599
FriOI	GRGCYC	1 cut(s)	1158
Fsp4HI	GCNGC	6 cut(s)	234, 477, 600, 897, 915, 1599
FspBI	CTAG	7 cut(s)	44, 81, 288, 704, 885, 1691, 1973
FspI	TGCGCA	1 cut(s)	2742
GlaI	GCGC	1 cut(s)	2742
GluI	GCNGC	6 cut(s)	234, 477, 600, 897, 915, 1599
GsaI	CCCAGC	3 cut(s)	304, 2572, 2694
GsuI	CTGGAG	2 cut(s)	2671, 3064
HaeIII	GGCC	6 cut(s)	71, 258, 1061, 1588, 2016, 2106
HapII	CCGG	3 cut(s)	259, 567, 765
HgaI	GACGC	3 cut(s)	488, 1478, 2636
HhaI	GCGC	1 cut(s)	2743
Hin1I	GRCGYC	1 cut(s)	1489
Hin6I	GCGC	1 cut(s)	2741
HinP1I	GCGC	1 cut(s)	2741
HincII	GTYRAC	6 cut(s)	340, 832, 987, 1774, 2626, 3183
HindII	GTYRAC	6 cut(s)	340, 832, 987, 1774, 2626, 3183
HindIII	AAGCTT	4 cut(s)	488, 1419, 2663, 2721
HpaII	CCGG	3 cut(s)	259, 567, 765
HphI	GGTGA	9 cut(s)	1258, 2177, 2285, 2311, 2414, 2561, 2774, 3044, 3097
Hpy166II	GTNNAC	7 cut(s)	340, 832, 987, 1774, 2626, 3061, 3183
Hpy8I	GTNNAC	7 cut(s)	340, 832, 987, 1774, 2626, 3061, 3183
Hpy99I	CGWCG	2 cut(s)	207, 284
HpyCH4III	ACNGT	6 cut(s)	52, 58, 646, 1736, 1995, 3187
HpyCH4IV	ACGT	4 cut(s)	282, 581, 1770, 2851
HpySE526I	ACGT	4 cut(s)	282, 581, 1770, 2851
Hsp92I	GRCGYC	1 cut(s)	1489
HspAI	GCGC	1 cut(s)	2741
Kzo9I	GATC	6 cut(s)	791, 1051, 1722, 2275, 2282, 3049
LguI	GCTCTTC	1 cut(s)	1889
LmnI	GCTCC	3 cut(s)	1768, 2227, 2427
Lsp1109I	GCAGC	5 cut(s)	463, 611, 883, 901, 1585
LweI	GCATC	5 cut(s)	661, 883, 2194, 2395, 2647
MaeI	CTAG	7 cut(s)	44, 81, 288, 704, 885, 1691, 1973
MaeII	ACGT	4 cut(s)	282, 581, 1770, 2851
MaeIII	GTNAC	5 cut(s)	46, 52, 283, 640, 3192
MalI	GATC	6 cut(s)	793, 1053, 1724, 2277, 2284, 3051
MboI	GATC	6 cut(s)	791, 1051, 1722, 2275, 2282, 3049
MfeI	CAATTG	2 cut(s)	2394, 2409
MhlI	GDGCHC	3 cut(s)	1158, 2224, 3108
MlyI	GAGTC	8 cut(s)	827, 1176, 1943, 1957, 2144, 2966, 3200, 3233
MmeI	TCCRAC	1 cut(s)	171
Mph1103I	ATGCAT	1 cut(s)	2187
MroXI	GAANNNNTTC	5 cut(s)	630, 1340, 1352, 1452, 2243
MseI	TTAA	6 cut(s)	609, 966, 1862, 2439, 2543, 2616
MslI	CAYNNNNRTG	4 cut(s)	507, 954, 2520, 3158
MspA1I	CMGCKG	2 cut(s)	300, 3083
MspI	CCGG	3 cut(s)	259, 567, 765
MspR9I	CCNGG	3 cut(s)	1057, 2280, 2477
MunI	CAATTG	2 cut(s)	2394, 2409
Mva1269I	GAATGC	1 cut(s)	1081
MvaI	CCWGG	3 cut(s)	1057, 2280, 2477
MvnI	CGCG	1 cut(s)	3175
NcoI	CCATGG	1 cut(s)	66
NdeI	CATATG	1 cut(s)	2992
NdeII	GATC	6 cut(s)	791, 1051, 1722, 2275, 2282, 3049
NlaIV	GGNNCC	3 cut(s)	547, 1507, 2105
NmeAIII	GCCGAG	1 cut(s)	424
NmuCI	GTSAC	2 cut(s)	283, 3192
NsbI	TGCGCA	1 cut(s)	2742
NsiI	ATGCAT	1 cut(s)	2187
NspI	RCATGY	5 cut(s)	1477, 1643, 2519, 2576, 2590
PagI	TCATGA	2 cut(s)	538, 3245
PasI	CCCWGGG	1 cut(s)	1056
PciI	ACATGT	1 cut(s)	1473
PciSI	GCTCTTC	1 cut(s)	1889
PctI	GAATGC	1 cut(s)	1081
PdmI	GAANNNNTTC	5 cut(s)	630, 1340, 1352, 1452, 2243
PfeI	GAWTC	5 cut(s)	1234, 1244, 1699, 2732, 3112
PfoI	TCCNGGA	1 cut(s)	2278
PkrI	GCNGC	6 cut(s)	235, 478, 601, 898, 916, 1600
PleI	GAGTC	8 cut(s)	827, 1175, 1942, 1956, 2143, 2966, 3199, 3232
PpsI	GAGTC	8 cut(s)	827, 1175, 1942, 1956, 2143, 2966, 3199, 3232
PscI	ACATGT	1 cut(s)	1473
PshBI	ATTAAT	2 cut(s)	1862, 2439
Psp124BI	GAGCTC	1 cut(s)	1158
Psp1406I	AACGTT	3 cut(s)	581, 1770, 2851
Psp6I	CCWGG	3 cut(s)	1055, 2278, 2475
PspEI	GGTNACC	1 cut(s)	640
PspFI	CCCAGC	3 cut(s)	300, 2568, 2690
PspGI	CCWGG	3 cut(s)	1055, 2278, 2475
PspN4I	GGNNCC	3 cut(s)	547, 1507, 2105
PspPI	GGNCC	7 cut(s)	256, 546, 1059, 1826, 2015, 2104, 3210
PstNI	CAGNNNCTG	2 cut(s)	1937, 2425
PvuII	CAGCTG	1 cut(s)	300
RsaI	GTAC	8 cut(s)	13, 22, 2006, 2064, 2391, 2453, 2585, 2927
RsaNI	GTAC	8 cut(s)	12, 21, 2005, 2063, 2390, 2452, 2584, 2926
RseI	CAYNNNNRTG	4 cut(s)	507, 954, 2520, 3158
SacI	GAGCTC	1 cut(s)	1158
SapI	GCTCTTC	1 cut(s)	1889
SaqAI	TTAA	6 cut(s)	609, 966, 1862, 2439, 2543, 2616
SatI	GCNGC	6 cut(s)	234, 477, 600, 897, 915, 1599
Sau3AI	GATC	6 cut(s)	791, 1051, 1722, 2275, 2282, 3049
Sau96I	GGNCC	7 cut(s)	256, 546, 1059, 1826, 2015, 2104, 3210
ScaI	AGTACT	1 cut(s)	2453
SchI	GAGTC	8 cut(s)	827, 1176, 1943, 1957, 2144, 2966, 3200, 3233
ScrFI	CCNGG	3 cut(s)	1057, 2280, 2477
SduI	GDGCHC	3 cut(s)	1158, 2224, 3108
SfaNI	GCATC	5 cut(s)	661, 883, 2194, 2395, 2647
SfcI	CTRYAG	2 cut(s)	359, 3186
SinI	GGWCC	3 cut(s)	546, 1826, 3210
SmiMI	CAYNNNNRTG	4 cut(s)	507, 954, 2520, 3158
SmlI	CTYRAG	3 cut(s)	242, 2633, 3228
SmoI	CTYRAG	3 cut(s)	242, 2633, 3228
SpeI	ACTAGT	1 cut(s)	80
SspI	AATATT	1 cut(s)	3206
SspMI	CTAG	7 cut(s)	44, 81, 288, 704, 885, 1691, 1973
SstI	GAGCTC	1 cut(s)	1158
StyD4I	CCNGG	3 cut(s)	1055, 2278, 2475
StyI	CCWWGG	4 cut(s)	66, 703, 1690, 2929
TaaI	ACNGT	6 cut(s)	52, 58, 646, 1736, 1995, 3187
TaiI	ACGT	4 cut(s)	285, 584, 1773, 2854
TaqI	TCGA	5 cut(s)	310, 507, 1170, 2290, 3145
TaqII	GACCGA	1 cut(s)	1814
TatI	WGTACW	3 cut(s)	11, 2451, 2583
TauI	GCSGC	1 cut(s)	236
TfiI	GAWTC	5 cut(s)	1234, 1244, 1699, 2732, 3112
Tru1I	TTAA	6 cut(s)	609, 966, 1862, 2439, 2543, 2616
Tru9I	TTAA	6 cut(s)	609, 966, 1862, 2439, 2543, 2616
TscAI	CASTG	6 cut(s)	376, 720, 1165, 1519, 1684, 2601
TseFI	GTSAC	2 cut(s)	283, 3192
TseI	GCWGC	5 cut(s)	476, 599, 896, 914, 1598
Tsp45I	GTSAC	2 cut(s)	283, 3192
TspGWI	ACGGA	2 cut(s)	160, 499
TspRI	CASTG	6 cut(s)	376, 720, 1165, 1519, 1684, 2601
VpaK11BI	GGWCC	3 cut(s)	546, 1826, 3210
VspI	ATTAAT	2 cut(s)	1862, 2439
XapI	RAATTY	5 cut(s)	945, 1384, 2168, 2241, 3234
XceI	RCATGY	5 cut(s)	1477, 1643, 2519, 2576, 2590
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2200
XmaJI	CCTAGG	2 cut(s)	703, 1690
XmnI	GAANNNNTTC	5 cut(s)	630, 1340, 1352, 1452, 2243
XspI	CTAG	7 cut(s)	44, 81, 288, 704, 885, 1691, 1973
ZrmI	AGTACT	1 cut(s)	2453
Zsp2I	ATGCAT	1 cut(s)	2187

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.