Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0013983

Physical Location & Seq

Genomic Coordinates Reverse (-)

11641040 .. 11652148

Gene Structure 11109 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00171330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3171 bp

ATGGCCTCTACTACTAGTCTCCAAATCCCCACTTTCCTCTCCCTCAAACCACTCCCTCAACGCCCCACCTTCGCCTTCTCCAACCACCCCACCTCCTTCTCTTCCGTCTCCTCCTCCTCCTCCCGCCCTCCACGCCGTCGTCTCACTCTCTCTCCCGCCCCCATTGCCGCCTCTCTCAAGGAAACTCTGGGCCGGATTGGAAAGACTTGGAACGACGTGACTAGCTTGAACAGCTGGGTTGTTCGAGACTACTACCGCCTTGTCAATTCTGTCAACTCTTTTGAGCCACAGCTACAGAGGCTCACTGATGACCAGCTTACTGCTAAAACTGCCGAGTTTCGGCGAAGATTGGGACAAGGAGAGACACTTGCGGATATTCAAGCTGAGGCTTTTGCTGTTGTTCGTGAAGCTGCGAAACGGAAGCTTGGTATGCGTCATTTCGATGTTCAGATTATTGGTGGGGCGGTGCTTCATGATGGGTCCATTGCAGAAATGAAGACCGGAGAAGGGAAAACGTTGGTTTCCACATTAGCAGCATATCTTAATGCCCTGACTGGCGAAGGTGTTCATGTGGTAACCGTAAATGATTACCTTGCTCAAAGAGATGCTGAGTGGATGGGTCGTGTTCATCGCTTCCTAGGTCTTTCAGTGGGTCTTATTCAGAGAGGGATGACAGCTGATAAGAGGAGATCCAACTATAGATGTGACATAACATACACCAATAATTCAGAACTTGGTTTTGACTATCTACGAGACAATCTTGCCGGAAACAGGGGAGAACTTGTTATGAGATCGCCAAAGCCATTTCATTTTGCAATAGTTGATGAAGTTGACTCTGTTCTTATTGATGAAGGAAGAAATCCATTGCTAATTAGTGGCGAGGCTAGTAAAGATGCTGCACGCTATCCTGTTGCTGCTAAAGTGGCTGAGTTGCTTGTGCGAGGAATTCATTACAATGTGGAACTTAAAGATTACTCAGTTGAGTTGACTGAAGAAGGAATAGCTCTTGCTGAGATGGCTCTTGAAACAAATGACTTATGGGATGAAAATGATCCCTGGGCCAGATTTGTGATGAATGCGTTGAAAGCTAAAGAGTTCTATCGGAAGGATGTGCAATATATTGTCAGAAATGGGAAGGCTCTCATAATCAATGAGCTCACTGGCAGAGTCGAAGATAAGAGAAGGTGGTCTGAGGGTATTCATCAAGCTGTGGAGGGTAAAGAAGGTTTGAAGATTCAGGCTGATTCAGTTGTTATCGCACAAATCACCTATCAATCTCTGTTCAAGCTCTACCCAAAGCTATCAGGGATGACTGGGACTGCAAAAACTGAAGAGAAAGAGTTCTTGAAAATGTTCCAGACACCAGTTATTGAAGTGCCAACAAATTTGCCAAACATTCGTAATGATTTACCTATTCAAGCTTTTGCGACTGCTCAGGGGAAATGGGAATATGTTCGTCAAGAAGTGGAATACATGTTCAGGCAGGGGCGTCCCGTTTTGGTTGGCACCACCAGTGTTGAGTATTCTGAACACCTGTCTGACCTATTGAGGGAACATAACATCCCACACAATGTTCTAAATGCACGGCCGAAGTATGCTGCAAGGGAGGCTGAAATTGTTGCCCAAGCTGGACGAAAACATGCCATTACTCTTTCTACCAATATGGCTGGCAGAGGCACTGACATAATCCTAGGAGGGAATCCTAAAATGCTTGCAAAAGAGATCATAGAAGACAGTTTGATTTCATTCCTGACACGAGAAGCTCCTAACGTTGACATAGATGGAGAGGCAATTTCACAAAAGGTATTGTCAAAGATAAAGGTCGGTCCATCATCATTAGCTTTGCTGGCTAAGACAGCATTAATGGCTAAATATGTTGGCAAAAATGAAGGGAAGAGCTGGACATATAAGGAGGCAAAATCCATGATATCAGAGTCTGTGGAAATGAGTCAATCAAGAGATTTGAAAGAGCTAGAGAGGCTTGTTGATGAACAGTCTGAGATGTACCCTCTTGGCCCTACAATAGCACTTGCTTATCTGTCAGTTTTGAAGGATTGCGAGGTACATTGTTTCAAAGAAGGGTCTGAAGTGAAAAGGCTTGGGGGCCTTCATGTGATTGGAACATCTTTGCACGAGTCTCGTAGAATAGATAACCAGCTTCGTGGCAGAGCAGGAAGGCAAGGTGATCCAGGATCAACTCGATTTATGGTGAGCTTGCAGGATGAGATGTTTCAAAAGTTCAATTTTGATACTGAGTGGGCTGTGAGACTTATTTCAAAGATTACTGATGATGAGGATGTACCAATTGAAGGTGATGCAATTGTGAGACAGCTCCTGTCCCTGCAAATTAATGCTGAAAAGTACTTTTTTGGCATAAGAAAGAGCCTGGTTGAGTTTGATGAAGTATTAGAGGTGCAAAGAAAGCATGTTTATGAACTTAGGCAGTCAATTTTAACTGGTGATAATGAAAGTTGTTCCCAGCATGTATTCCAGTACATGCAAGCAGTGGTAGATGAAATTGTTTTTAAAAATGTTGACGCTCTCAAGCATCCAAGAAACTGGAGTTTGAATAAGCTTTTGAAGGAGTTTATGGCGATTGCTGGGAAACTATTGGATGATTCATTTGCAGGAATTACTGAGGAAGCTTTGTTAGAATCTCTTGCGCAATCACATGAACTGAACTCTAAAGATATTGATGACATTCACCTTCCAAACTTGCCAAGACCTCCTAATGCCTTCAGAGGTATCCGCAAGAAGAACTCTTCATTGAAACGTTGGCTTGCTATATGCTCGGATGATTTGACCAAGAATGGAAGGTATCATGCTACTACTAACCTCCTCCGCAAGTACCTTGGAGACTATTTGATTGCTTCGTATTTGGATGTTGTTCAAGACTCTGGTTATGATGATACATATGTGAAGGAAGTTGAAAGAGCAGTTATTGTGAAAACCCTTGATTGTTTCTGGAGGGATCACCTTGTAAACATGAACAGGCTAAGTTCAGCGGTGAATGTAAGGAGTTTTGGGCACAGGAATCCTCTTGAAGAATACAAGATTGATGGTTGTCGATTTTTCATATCTATGCTTAGTGCAACGCGAAGGTTGACTGTAGAGTCACTTTTGCAATATTGGTCCTCCCCAATGGAGTCTCAAGAAATTTTTGTATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0006996 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0009657 GO:0009658 GO:0009987 GO:0010109 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019222 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0031323 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW WD40 zf-RING_UBOX

Protein Analysis

1056

Amino Acids

119.2

Weight (kDa)

6.58

Isoelectric Point (pI)

37.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	78 - 448	1.5e-116	SecA DEAD-like domain
SecA_PP_bind	PF01043	299 - 405	5.3e-29	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	464 - 751	9.4e-66	SecA P-loop domain
SecA_SW	PF07516	768 - 1042	3.6e-42	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21650 AT1G21650 AT1G21650
fragaria_vesca	FvH4_2g27750
malus_domestica	MD00G1100600.v1.1
prunus_persica	Prupe.1G490400_v2.0.a1
pyrus_communis	pycom15g30570
rosa_chinensis	RchiOBHm_Chr6g0296621
rosa_laevigata	RLG00000011676
rosa_multiflora	Rmu_sc0001741.1_g000019
rosa_roxburghii	Rroxscaffold_7G00171330 Rroxscaffold_7G00178770
rosa_rugosa	Rorug06G0263700
rosa_samantha	Rh6AG375100 Rh6CG388700
rosa_wichuraiana	Rw6G032720 Rw6G032810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3112
Acc16I	TGCGCA	1 cut(s)	2664
AccB1I	GGYRCC	1 cut(s)	1505
AccII	CGCG	1 cut(s)	3097
AciI	CCGC	9 cut(s)	124, 156, 168, 256, 371, 464, 2749, 2842, 3005
AclI	AACGTT	3 cut(s)	515, 1770, 2773
AclWI	GGATC	5 cut(s)	684, 1046, 2180, 2200, 2979
AcoI	YGGCCR	1 cut(s)	1586
AcsI	RAATTY	3 cut(s)	945, 1384, 3156
AcuI	CTGAAG	4 cut(s)	1011, 1350, 2106, 2722
AcyI	GRCGYC	1 cut(s)	1489
AfaI	GTAC	6 cut(s)	2006, 2064, 2301, 2363, 2495, 2849
AfiI	CCNNNNNNNGG	5 cut(s)	339, 507, 771, 1549, 2309
AflIII	ACRYGT	1 cut(s)	1473
AhlI	ACTAGT	1 cut(s)	14
AjiI	CACGTC	1 cut(s)	217
AjnI	CCWGG	3 cut(s)	1055, 2188, 2385
AloI	GAACNNNNNNTCC	4 cut(s)	1460, 1492, 1545, 1577
Alw21I	GWGCWC	1 cut(s)	1158
AlwI	GGATC	5 cut(s)	684, 1046, 2180, 2200, 2979
AlwNI	CAGNNNCTG	2 cut(s)	1937, 2335
AoxI	GGCC	6 cut(s)	3, 190, 1059, 1586, 2014, 2104
ApeKI	GCWGC	5 cut(s)	410, 533, 896, 914, 1598
ApoI	RAATTY	3 cut(s)	945, 1384, 3156
AseI	ATTAAT	2 cut(s)	1862, 2349
Asp700I	GAANNNNTTC	4 cut(s)	564, 1340, 1352, 1452
AspA2I	CCTAGG	2 cut(s)	637, 1690
AspLEI	GCGC	1 cut(s)	2665
AspS9I	GGNCC	7 cut(s)	190, 480, 1059, 1826, 2015, 2104, 3132
AsuHPI	GGTGA	8 cut(s)	1258, 2195, 2221, 2324, 2471, 2696, 2966, 3019
AvaII	GGWCC	3 cut(s)	480, 1826, 3132
AvrII	CCTAGG	2 cut(s)	637, 1690
BaeGI	GKGCMC	1 cut(s)	3030
BaeI	ACNNNNGTAYC	2 cut(s)	2901, 2934
BanI	GGYRCC	1 cut(s)	1505
BanII	GRGCYC	1 cut(s)	1158
BauI	CACGAG	2 cut(s)	1755, 2132
BbsI	GAAGAC	2 cut(s)	503, 1737
Bbv12I	GWGCWC	1 cut(s)	1158
BbvCI	CCTCAGC	1 cut(s)	384
BbvI	GCAGC	5 cut(s)	397, 545, 883, 901, 1585
BccI	CCATC	6 cut(s)	470, 610, 1009, 1775, 1837, 3053
BceAI	ACGGC	2 cut(s)	120, 1601
BcgI	CGANNNNNNTGC	2 cut(s)	2120, 2154
BciT130I	CCWGG	3 cut(s)	1057, 2190, 2387
BciVI	GTATCC	1 cut(s)	2756
BcuI	ACTAGT	1 cut(s)	14
BfaI	CTAG	6 cut(s)	15, 222, 638, 885, 1691, 1973
BfmI	CTRYAG	3 cut(s)	293, 697, 3108
BfuI	GTATCC	1 cut(s)	2756
BisI	GCNGC	6 cut(s)	168, 411, 534, 897, 915, 1599
BlnI	CCTAGG	2 cut(s)	637, 1690
BlsI	GCNGC	6 cut(s)	169, 412, 535, 898, 916, 1600
BmcAI	AGTACT	1 cut(s)	2363
Bme1390I	CCNGG	3 cut(s)	1057, 2190, 2387
Bme18I	GGWCC	3 cut(s)	480, 1826, 3132
BmgBI	CACGTC	1 cut(s)	217
BmgT120I	GGNCC	7 cut(s)	190, 480, 1059, 1826, 2015, 2104, 3132
BmiI	GGNNCC	3 cut(s)	481, 1507, 2105
BmrFI	CCNGG	3 cut(s)	1057, 2190, 2387
BmrI	ACTGGG	1 cut(s)	1323
BmsI	GCATC	4 cut(s)	595, 883, 2305, 2557
BmuI	ACTGGG	1 cut(s)	1323
BpiI	GAAGAC	2 cut(s)	503, 1737
BpmI	CTGGAG	2 cut(s)	2581, 2986
Bpu10I	CCTNAGC	2 cut(s)	384, 1434
BpuEI	CTTGAG	3 cut(s)	161, 2528, 3135
BsaHI	GRCGYC	1 cut(s)	1489
BsaJI	CCNNGG	5 cut(s)	637, 1055, 1056, 1690, 2851
BsaWI	WCCGGW	1 cut(s)	500
BsaXI	ACNNNNNCTCC	6 cut(s)	77, 107, 136, 166, 768, 798
Bsc4I	CCNNNNNNNGG	5 cut(s)	339, 507, 771, 1549, 2309
Bse1I	ACTGG	8 cut(s)	559, 1165, 1318, 1364, 1512, 2461, 2491, 2564
Bse3DI	GCAATG	3 cut(s)	162, 483, 863
BseBI	CCWGG	3 cut(s)	1057, 2190, 2387
BseDI	CCNNGG	5 cut(s)	637, 1055, 1056, 1690, 2851
BseLI	CCNNNNNNNGG	5 cut(s)	339, 507, 771, 1549, 2309
BseMI	GCAATG	3 cut(s)	162, 483, 863
BseNI	ACTGG	8 cut(s)	559, 1165, 1318, 1364, 1512, 2461, 2491, 2564
BseRI	GAGGAG	6 cut(s)	100, 103, 106, 109, 700, 2828
BseSI	GKGCMC	1 cut(s)	3030
BseX3I	CGGCCG	1 cut(s)	1586
BseXI	GCAGC	5 cut(s)	397, 545, 883, 901, 1585
BseYI	CCCAGC	3 cut(s)	234, 2478, 2600
BsgI	GTGCAG	1 cut(s)	882
Bsh1236I	CGCG	1 cut(s)	3097
Bsh1285I	CGRYCG	1 cut(s)	1589
BshFI	GGCC	6 cut(s)	5, 192, 1061, 1588, 2016, 2106
BshNI	GGYRCC	1 cut(s)	1505
BsiEI	CGRYCG	1 cut(s)	1589
BsiHKAI	GWGCWC	1 cut(s)	1158
BsiSI	CCGG	3 cut(s)	193, 501, 765
BslFI	GGGAC	4 cut(s)	366, 1330, 1476, 2323
BslI	CCNNNNNNNGG	5 cut(s)	339, 507, 771, 1549, 2309
BsmBI	CGTCTC	2 cut(s)	112, 146
BsmFI	GGGAC	4 cut(s)	366, 1330, 1476, 2323
BsmI	GAATGC	1 cut(s)	1081
BsnI	GGCC	6 cut(s)	5, 192, 1061, 1588, 2016, 2106
Bsp1286I	GDGCHC	2 cut(s)	1158, 3030
Bsp143I	GATC	7 cut(s)	689, 791, 1051, 1722, 2185, 2192, 2971
BspACI	CCGC	9 cut(s)	124, 156, 168, 256, 371, 464, 2749, 2842, 3005
BspANI	GGCC	6 cut(s)	5, 192, 1061, 1588, 2016, 2106
BspFNI	CGCG	1 cut(s)	3097
BspHI	TCATGA	2 cut(s)	472, 3167
BspLI	GGNNCC	3 cut(s)	481, 1507, 2105
BspPI	GGATC	5 cut(s)	684, 1046, 2180, 2200, 2979
BspQI	GCTCTTC	1 cut(s)	1889
BspT107I	GGYRCC	1 cut(s)	1505
BsrDI	GCAATG	3 cut(s)	162, 483, 863
BsrI	ACTGG	8 cut(s)	559, 1165, 1318, 1364, 1512, 2461, 2491, 2564
BssECI	CCNNGG	5 cut(s)	637, 1055, 1056, 1690, 2851
BssMI	GATC	7 cut(s)	689, 791, 1051, 1722, 2185, 2192, 2971
BssNI	GRCGYC	1 cut(s)	1489
BssSI	CACGAG	2 cut(s)	1755, 2132
BssT1I	CCWWGG	3 cut(s)	637, 1690, 2851
Bst2BI	CACGAG	2 cut(s)	1755, 2132
Bst2UI	CCWGG	3 cut(s)	1057, 2190, 2387
Bst4CI	ACNGT	4 cut(s)	580, 1736, 1995, 3109
Bst6I	CTCTTC	4 cut(s)	106, 1326, 1889, 2767
BstACI	GRCGYC	1 cut(s)	1489
BstC8I	GCNNGC	7 cut(s)	901, 1669, 1713, 1848, 2216, 2502, 2781
BstEII	GGTNACC	1 cut(s)	574
BstFNI	CGCG	1 cut(s)	3097
BstHHI	GCGC	1 cut(s)	2665
BstKTI	GATC	7 cut(s)	692, 794, 1054, 1725, 2188, 2195, 2974
BstMBI	GATC	7 cut(s)	689, 791, 1051, 1722, 2185, 2192, 2971
BstMCI	CGRYCG	1 cut(s)	1589
BstNI	CCWGG	3 cut(s)	1057, 2190, 2387
BstNSI	RCATGY	5 cut(s)	1477, 1643, 2429, 2486, 2500
BstPI	GGTNACC	1 cut(s)	574
BstSCI	CCNGG	3 cut(s)	1055, 2188, 2385
BstSFI	CTRYAG	3 cut(s)	293, 697, 3108
BstSLI	GKGCMC	1 cut(s)	3030
BstUI	CGCG	1 cut(s)	3097
BstV1I	GCAGC	5 cut(s)	397, 545, 883, 901, 1585
BstV2I	GAAGAC	2 cut(s)	503, 1737
BstX2I	RGATCY	1 cut(s)	689
BstXI	CCANNNNNNTGG	2 cut(s)	2162, 2559
BstYI	RGATCY	1 cut(s)	689
BstZI	CGGCCG	1 cut(s)	1586
BsuI	GTATCC	1 cut(s)	2756
BsuRI	GGCC	6 cut(s)	5, 192, 1061, 1588, 2016, 2106
BtgZI	GCGATG	1 cut(s)	614
BtrI	CACGTC	1 cut(s)	217
BtsI	GCAGTG	1 cut(s)	2511
BtsIMutI	CAGTG	6 cut(s)	303, 654, 1158, 1519, 1677, 2511
Cac8I	GCNNGC	7 cut(s)	901, 1669, 1713, 1848, 2216, 2502, 2781
CaiI	CAGNNNCTG	2 cut(s)	1937, 2335
CciI	TCATGA	2 cut(s)	472, 3167
CfoI	GCGC	1 cut(s)	2665
Cfr13I	GGNCC	7 cut(s)	190, 480, 1059, 1826, 2015, 2104, 3132
CseI	GACGC	3 cut(s)	422, 1478, 2546
Csp6I	GTAC	6 cut(s)	2005, 2063, 2300, 2362, 2494, 2848
CspCI	CAANNNNNGTGG	2 cut(s)	1498, 1533
CviQI	GTAC	6 cut(s)	2005, 2063, 2300, 2362, 2494, 2848
DpnI	GATC	7 cut(s)	691, 793, 1053, 1724, 2187, 2194, 2973
DpnII	GATC	7 cut(s)	689, 791, 1051, 1722, 2185, 2192, 2971
DraI	TTTAAA	1 cut(s)	2527
DrdI	GACNNNNNNGTC	1 cut(s)	3112
DseDI	GACNNNNNNGTC	1 cut(s)	3112
EaeI	YGGCCR	1 cut(s)	1586
EagI	CGGCCG	1 cut(s)	1586
Eam1104I	CTCTTC	4 cut(s)	106, 1326, 1889, 2767
EarI	CTCTTC	4 cut(s)	106, 1326, 1889, 2767
Ecl136II	GAGCTC	1 cut(s)	1156
EclXI	CGGCCG	1 cut(s)	1586
Eco130I	CCWWGG	3 cut(s)	637, 1690, 2851
Eco24I	GRGCYC	1 cut(s)	1158
Eco32I	GATATC	1 cut(s)	1929
Eco47I	GGWCC	3 cut(s)	480, 1826, 3132
Eco52I	CGGCCG	1 cut(s)	1586
Eco53kI	GAGCTC	1 cut(s)	1156
Eco57I	CTGAAG	4 cut(s)	1011, 1350, 2106, 2722
Eco91I	GGTNACC	1 cut(s)	574
EcoICRI	GAGCTC	1 cut(s)	1156
EcoO109I	RGGNCCY	1 cut(s)	2104
EcoO65I	GGTNACC	1 cut(s)	574
EcoRI	GAATTC	1 cut(s)	945
EcoRII	CCWGG	3 cut(s)	1055, 2188, 2385
EcoRV	GATATC	1 cut(s)	1929
EcoT14I	CCWWGG	3 cut(s)	637, 1690, 2851
EcoT38I	GRGCYC	1 cut(s)	1158
ErhI	CCWWGG	3 cut(s)	637, 1690, 2851
Esp3I	CGTCTC	2 cut(s)	112, 146
FaqI	GGGAC	4 cut(s)	366, 1330, 1476, 2323
FauI	CCCGC	2 cut(s)	131, 163
FauNDI	CATATG	1 cut(s)	2914
Fnu4HI	GCNGC	6 cut(s)	168, 411, 534, 897, 915, 1599
FriOI	GRGCYC	1 cut(s)	1158
Fsp4HI	GCNGC	6 cut(s)	168, 411, 534, 897, 915, 1599
FspBI	CTAG	6 cut(s)	15, 222, 638, 885, 1691, 1973
FspI	TGCGCA	1 cut(s)	2664
GlaI	GCGC	1 cut(s)	2664
GluI	GCNGC	6 cut(s)	168, 411, 534, 897, 915, 1599
GsaI	CCCAGC	3 cut(s)	238, 2482, 2604
GsuI	CTGGAG	2 cut(s)	2581, 2986
HaeIII	GGCC	6 cut(s)	5, 192, 1061, 1588, 2016, 2106
HapII	CCGG	3 cut(s)	193, 501, 765
HgaI	GACGC	3 cut(s)	422, 1478, 2546
HhaI	GCGC	1 cut(s)	2665
Hin1I	GRCGYC	1 cut(s)	1489
Hin6I	GCGC	1 cut(s)	2663
HinP1I	GCGC	1 cut(s)	2663
HincII	GTYRAC	6 cut(s)	274, 832, 987, 1774, 2536, 3105
HindII	GTYRAC	6 cut(s)	274, 832, 987, 1774, 2536, 3105
HindIII	AAGCTT	4 cut(s)	422, 1419, 2573, 2643
HpaII	CCGG	3 cut(s)	193, 501, 765
HphI	GGTGA	8 cut(s)	1258, 2195, 2221, 2324, 2471, 2696, 2966, 3019
Hpy166II	GTNNAC	7 cut(s)	274, 832, 987, 1774, 2536, 2983, 3105
Hpy8I	GTNNAC	7 cut(s)	274, 832, 987, 1774, 2536, 2983, 3105
Hpy99I	CGWCG	2 cut(s)	141, 218
HpyCH4III	ACNGT	4 cut(s)	580, 1736, 1995, 3109
HpyCH4IV	ACGT	4 cut(s)	216, 515, 1770, 2773
HpySE526I	ACGT	4 cut(s)	216, 515, 1770, 2773
Hsp92I	GRCGYC	1 cut(s)	1489
HspAI	GCGC	1 cut(s)	2663
Kzo9I	GATC	7 cut(s)	689, 791, 1051, 1722, 2185, 2192, 2971
LguI	GCTCTTC	1 cut(s)	1889
LmnI	GCTCC	2 cut(s)	1768, 2337
Lsp1109I	GCAGC	5 cut(s)	397, 545, 883, 901, 1585
LweI	GCATC	4 cut(s)	595, 883, 2305, 2557
MaeI	CTAG	6 cut(s)	15, 222, 638, 885, 1691, 1973
MaeII	ACGT	4 cut(s)	216, 515, 1770, 2773
MaeIII	GTNAC	4 cut(s)	217, 574, 704, 3114
MalI	GATC	7 cut(s)	691, 793, 1053, 1724, 2187, 2194, 2973
MboI	GATC	7 cut(s)	689, 791, 1051, 1722, 2185, 2192, 2971
MfeI	CAATTG	2 cut(s)	2304, 2319
MflI	RGATCY	1 cut(s)	689
MhlI	GDGCHC	2 cut(s)	1158, 3030
MlyI	GAGTC	8 cut(s)	827, 1176, 1943, 1957, 2144, 2888, 3122, 3155
MmeI	TCCRAC	2 cut(s)	105, 717
MroXI	GAANNNNTTC	4 cut(s)	564, 1340, 1352, 1452
MseI	TTAA	6 cut(s)	543, 966, 1862, 2349, 2453, 2526
MslI	CAYNNNNRTG	4 cut(s)	441, 954, 2430, 3080
MspA1I	CMGCKG	3 cut(s)	234, 677, 3005
MspI	CCGG	3 cut(s)	193, 501, 765
MspR9I	CCNGG	3 cut(s)	1057, 2190, 2387
MunI	CAATTG	2 cut(s)	2304, 2319
Mva1269I	GAATGC	1 cut(s)	1081
MvaI	CCWGG	3 cut(s)	1057, 2190, 2387
MvnI	CGCG	1 cut(s)	3097
NdeI	CATATG	1 cut(s)	2914
NdeII	GATC	7 cut(s)	689, 791, 1051, 1722, 2185, 2192, 2971
NlaIV	GGNNCC	3 cut(s)	481, 1507, 2105
NmeAIII	GCCGAG	1 cut(s)	358
NmuCI	GTSAC	3 cut(s)	217, 704, 3114
NsbI	TGCGCA	1 cut(s)	2664
NspI	RCATGY	5 cut(s)	1477, 1643, 2429, 2486, 2500
PagI	TCATGA	2 cut(s)	472, 3167
PasI	CCCWGGG	1 cut(s)	1056
PciI	ACATGT	1 cut(s)	1473
PciSI	GCTCTTC	1 cut(s)	1889
PctI	GAATGC	1 cut(s)	1081
PdmI	GAANNNNTTC	4 cut(s)	564, 1340, 1352, 1452
PfeI	GAWTC	6 cut(s)	1234, 1244, 1699, 2618, 2654, 3034
PfoI	TCCNGGA	1 cut(s)	2188
PkrI	GCNGC	6 cut(s)	169, 412, 535, 898, 916, 1600
PleI	GAGTC	8 cut(s)	827, 1175, 1942, 1956, 2143, 2888, 3121, 3154
PpsI	GAGTC	8 cut(s)	827, 1175, 1942, 1956, 2143, 2888, 3121, 3154
PscI	ACATGT	1 cut(s)	1473
PshBI	ATTAAT	2 cut(s)	1862, 2349
Psp124BI	GAGCTC	1 cut(s)	1158
Psp1406I	AACGTT	3 cut(s)	515, 1770, 2773
Psp6I	CCWGG	3 cut(s)	1055, 2188, 2385
PspEI	GGTNACC	1 cut(s)	574
PspFI	CCCAGC	3 cut(s)	234, 2478, 2600
PspGI	CCWGG	3 cut(s)	1055, 2188, 2385
PspN4I	GGNNCC	3 cut(s)	481, 1507, 2105
PspPI	GGNCC	7 cut(s)	190, 480, 1059, 1826, 2015, 2104, 3132
PstNI	CAGNNNCTG	2 cut(s)	1937, 2335
PsuI	RGATCY	1 cut(s)	689
PvuII	CAGCTG	2 cut(s)	234, 677
RsaI	GTAC	6 cut(s)	2006, 2064, 2301, 2363, 2495, 2849
RsaNI	GTAC	6 cut(s)	2005, 2063, 2300, 2362, 2494, 2848
RseI	CAYNNNNRTG	4 cut(s)	441, 954, 2430, 3080
SacI	GAGCTC	1 cut(s)	1158
SapI	GCTCTTC	1 cut(s)	1889
SaqAI	TTAA	6 cut(s)	543, 966, 1862, 2349, 2453, 2526
SatI	GCNGC	6 cut(s)	168, 411, 534, 897, 915, 1599
Sau3AI	GATC	7 cut(s)	689, 791, 1051, 1722, 2185, 2192, 2971
Sau96I	GGNCC	7 cut(s)	190, 480, 1059, 1826, 2015, 2104, 3132
ScaI	AGTACT	1 cut(s)	2363
SchI	GAGTC	8 cut(s)	827, 1176, 1943, 1957, 2144, 2888, 3122, 3155
ScrFI	CCNGG	3 cut(s)	1057, 2190, 2387
SduI	GDGCHC	2 cut(s)	1158, 3030
SfaNI	GCATC	4 cut(s)	595, 883, 2305, 2557
SfcI	CTRYAG	3 cut(s)	293, 697, 3108
SinI	GGWCC	3 cut(s)	480, 1826, 3132
SmiMI	CAYNNNNRTG	4 cut(s)	441, 954, 2430, 3080
SmlI	CTYRAG	3 cut(s)	176, 2543, 3150
SmoI	CTYRAG	3 cut(s)	176, 2543, 3150
SpeI	ACTAGT	1 cut(s)	14
SsiI	CCGC	9 cut(s)	124, 156, 168, 256, 371, 464, 2749, 2842, 3005
SspI	AATATT	1 cut(s)	3128
SspMI	CTAG	6 cut(s)	15, 222, 638, 885, 1691, 1973
SstI	GAGCTC	1 cut(s)	1158
StyD4I	CCNGG	3 cut(s)	1055, 2188, 2385
StyI	CCWWGG	3 cut(s)	637, 1690, 2851
TaaI	ACNGT	4 cut(s)	580, 1736, 1995, 3109
TaiI	ACGT	4 cut(s)	219, 518, 1773, 2776
TaqI	TCGA	5 cut(s)	244, 441, 1170, 2200, 3067
TaqII	GACCGA	1 cut(s)	1814
TatI	WGTACW	2 cut(s)	2361, 2493
TauI	GCSGC	1 cut(s)	170
TfiI	GAWTC	6 cut(s)	1234, 1244, 1699, 2618, 2654, 3034
Tru1I	TTAA	6 cut(s)	543, 966, 1862, 2349, 2453, 2526
Tru9I	TTAA	6 cut(s)	543, 966, 1862, 2349, 2453, 2526
TscAI	CASTG	6 cut(s)	310, 654, 1165, 1519, 1684, 2511
TseFI	GTSAC	3 cut(s)	217, 704, 3114
TseI	GCWGC	5 cut(s)	410, 533, 896, 914, 1598
Tsp45I	GTSAC	3 cut(s)	217, 704, 3114
TspGWI	ACGGA	2 cut(s)	94, 433
TspRI	CASTG	6 cut(s)	310, 654, 1165, 1519, 1684, 2511
VpaK11BI	GGWCC	3 cut(s)	480, 1826, 3132
VspI	ATTAAT	2 cut(s)	1862, 2349
XapI	RAATTY	3 cut(s)	945, 1384, 3156
XceI	RCATGY	5 cut(s)	1477, 1643, 2429, 2486, 2500
XmaJI	CCTAGG	2 cut(s)	637, 1690
XmnI	GAANNNNTTC	4 cut(s)	564, 1340, 1352, 1452
XspI	CTAG	6 cut(s)	15, 222, 638, 885, 1691, 1973
ZrmI	AGTACT	1 cut(s)	2363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.