Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0013983

Physical Location & Seq

Genomic Coordinates Reverse (-)

21325043 .. 21335230

Gene Structure 10188 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1100600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3177 bp

ATGGCTATGGCTGCCCTGCCTTCTCTCCAAAACCCCTATTTCCTATCCCTTAAACCTCTCTCACAACTCTCCACCTCCGTCCTCCCCGACCAAGCCCGCTTCTTTTCTTCGTTTTCTACCTTCTCTCGGCCTCCACGACGTCGTCTCAGGCTCACAACCAAACCCATTGCTGCCTCTATTAAGGAAAACTTGGGTCTTATAAGGAAAACTTGGAGTGATGTAACTAGTTTGAACAACTGGGTTGTTCGGGACTACTACCGCCTTGTGAAGTCTGTAAATGCGCTCGAGCCTCAAGTACAGAGTCTCTCTGATGACCAGCTTACTGCTAAAACTGCAGAGTTCCGGCAAAGGCTGGGAAAGGGGGAGACACTTGCTGATATCCAAGCTGAGGCTTTTGCTGTTGTTCGTGAAGCTGCAAAAAGGAAGCTTGGTATGCGTCATTTCGATGTGCAGATTATTGGTGGGGCGGTGCTTCATGATGGGTCCATTGCAGAAATGAAAACAGGAGAGGGAAAAACGTTGGTTTCCACCTTGGCGGCATATCTTAATGCCCTGACAGGTGAAGGCGTTCATGTGGTAACCGTAAACGATTACCTTGCACAACGAGATGCTGACTGGATGGGTCGTGTTCATCGCTTCTTAGGCCTTACTGTGGGTCTTGTTCAGAGGGGGATGACAGCTGAAGAGAGGAGGTCCAACTATAGCTGTGACATAACATACACTAATAACTCAGAACTTGGTTTTGACTATCTACGAGATAACCTTGCTGGAAACAGTGGACAGCTTGTTATGAAATGGCCAAAGCCATTTCATTTTGCAATAGTTGATGAAGTTGACTCAGTTCTTATTGACGAAGGACGAAATCCATTGCTAATTAGTGGGGAGGCTAGTAAAGATGCTGCACGCTATCCTGTGGCTGCTAAAGTGGCAGATTTGCTTGTGCGAGGAATTCATTACAATGTAGAACTTAAAGATAATTCAGTTGAGTTGACTGAAGAAGGAATAGCTCTTGCTGAGATGGCCCTTGAAACAAATGACTTGTGGGATGAAAATGATCCTTGGGCTAGATTTGTGATGAATGCGTTGAAGGCTAAAGAGTTCTATCGTCAAGGTGTGCAATACATTGTCAGAAATGGGAAGGCTCTCATAATAAATGAGCTGACTGGCAGAGTTGAGGAGAAGAGAAGGTGGTCTGAGGGGATTCATCAGGCTGTGGAAGCTAAAGAAGGCTTGAAGATTCAGGCTGATTCGGTTGTTGTGGCACAAATCACTTATCAATCTTTATTCAAGCTCTACCCAAAACTGTCAGGAATGACTGGAACTGCAAAAACAGAAGAGAAAGAGTTCTTGAAGATGTTTCAAGTGCCAGTCATTGAAATGCCAACAAATCTTCCAAACATTCGTAATGATTTACCAATTCAAGCTTTTGCGACTGCTCAAGGAAAGTGGGAATATGTTCGACAAGAAGTGGAGTACATGTTCAGACAGGGGCGACCTGTTTTGGTTGGAACTACCAGTGTTGAGAATTCCGAGTACTTGTCTGATCTGCTGAGGGAACAGAATATCCCCCACAATATTCTAAATGCACGGCCTAAGTATGCTGCAAGGGAGGCTGAAATTGTTGCCCAAGCAGGAAGAAAATATGCCATTACCATTTCTACCAATATGGCTGGCAGAGGCACTGACATAATTCTAGGAGGAAATCCAAAAATGCTTGCAAAAGAGATCATAGAAGACAGTTTAATTTCGTTCCTGACACGAGAAGCTCCAAATGTTGATGTTGATGGAGAGGCAATTTCACAAAAGGTATTGTCCAAGATAAAGGTCGGTCCATCATCATTAGCTTTTCTTGCTAAGACAGCACTAATGGCCAAGTATGTTTGCAAAAATGAAGGCAAGAGCTGGACATATAAGGAGGCAAAATCTATGATATCAGAGTCAGTGGAAATGAGTCAGTCAAAAGATTTGAAAGATTTAGAGACGCTTGTTGATGAACAGTCAGAGATGTACCCTCTTGGCCCTACAATTGCACTTGCTTATCTATCTGTTCTGAAGGATTGCGAGGTACACTGTTTCAAAGAAGGGTCTGAAGTAAAAAATCTTGGGGGTCTTCATGTGATAGGAACATCTTTGCATGAGTCACGGAGAATAGATAACCAGCTTCGTGGAAGAGCTGGAAGGCAAGGTGATCCTGGATCCACACGATTTATGGTGAGCTTGCAGGATGAGATGTTTCAAAAGTTTAATTTTGACACTGAGTGGGCTGTGAGACTTATTTCCAAAATTACAAATGATGAGGATATGCCAATTGAAGGTGGTGCAATTGTTAAACAGCTACTGGCCCTGCAAGTTAATGCAGAGAAGTACTTCTTTGGCATAAGGAAGAGCCTTGTTGAGTTTGATGAAGTATTAGAGGTGCAGAGAAAGCATGTCTATGAACTTAGACAGTCCATTTTAACGGGTGATGATGAAAGTTGTTCCCAGAACATTTACCAGTACATGCAGGCAGTGGTAGATGAAATTGTCTTTGGAAACGTTGATGCACTGAAGGTACATCCAAGAAATTGGAATTTGGGGAAGCTTTTGAAGGAGTTTATGACAATTTCTGGGAAACTACTGGATGATTCATTTACAGGCATTACAGAGGAAGTTTTACTAAAATCTCTAGCAGACTCACATGAACTAAACTCTAGAAATATTCATGACGTTCACCTTCCAAACTTGCCAAGACCTCCAAATGCCTTCAGAGGAATCCGCAAGAAGAGCTCTTCATTGAAACGCTGGCTTGCTATATGCTCTGATGATATGACTAAGAATGGACGGTACCATGCTACTACTAGCCTCCTCCGCAAATACCTTGGAGATCTTTTAATTGCTTCATACTTGGATGTTATTCAAGAATCTGGTTATGATGATGGACATGTGAAGGAAGTGGAAAGAGCAGTTATTGTGAAAACCCTTGATTGTTTCTGGAGGGATCATCTCGTAAACATGAACAGGCTTAGCTCAGCGGTAAACGTAAGGAGTTTCGGGCACAGGAATCCTCTTGAAGAATACAAAATTGATGGTTGTCGATTTTTCATATCTATGCTTAGTGCAACACGAAGGTTGACTGTAGAATCTCTTGTGCAATATTGGTCATCCTCAATGGAGTCCCAAGAAATTTTTGTATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0006996 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0009657 GO:0009658 GO:0009987 GO:0010109 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019222 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0031323 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW WD40 zf-RING_UBOX

Protein Analysis

1059

Amino Acids

119.17

Weight (kDa)

6.43

Isoelectric Point (pI)

36.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	78 - 449	4.9e-118	SecA DEAD-like domain
SecA_PP_bind	PF01043	300 - 406	2e-28	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	465 - 752	9.4e-67	SecA P-loop domain
SecA_SW	PF07516	769 - 1044	3.1e-41	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21650 AT1G21650 AT1G21650
fragaria_vesca	FvH4_2g27750
malus_domestica	MD00G1100600.v1.1
prunus_persica	Prupe.1G490400_v2.0.a1
pyrus_communis	pycom15g30570
rosa_chinensis	RchiOBHm_Chr6g0296621
rosa_laevigata	RLG00000011676
rosa_multiflora	Rmu_sc0001741.1_g000019
rosa_roxburghii	Rroxscaffold_7G00171330 Rroxscaffold_7G00178770
rosa_rugosa	Rorug06G0263700
rosa_samantha	Rh6AG375100 Rh6CG388700
rosa_wichuraiana	Rw6G032720 Rw6G032810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	200
AatII	GACGTC	1 cut(s)	142
Acc65I	GGTACC	1 cut(s)	2823
AccB1I	GGYRCC	1 cut(s)	2823
AciI	CCGC	7 cut(s)	97, 259, 467, 536, 2755, 2848, 3011
AclI	AACGTT	2 cut(s)	518, 2535
AclWI	GGATC	5 cut(s)	1049, 2183, 2190, 2203, 2985
AcoI	YGGCCR	2 cut(s)	797, 1869
AcsI	RAATTY	4 cut(s)	948, 1525, 2569, 3162
AcuI	CTGAAG	6 cut(s)	702, 1014, 2072, 2109, 2567, 2728
AcyI	GRCGYC	1 cut(s)	139
AdeI	CACNNNGTG	1 cut(s)	2259
AfaI	GTAC	9 cut(s)	297, 1475, 1535, 2009, 2067, 2366, 2498, 2553, 2825
AfiI	CCNNNNNNNGG	4 cut(s)	126, 388, 652, 2312
AflIII	ACRYGT	2 cut(s)	1476, 2920
AhlI	ACTAGT	1 cut(s)	224
AjnI	CCWGG	1 cut(s)	2191
AjuI	GAANNNNNNNTTGG	2 cut(s)	2511, 2543
AloI	GAACNNNNNNTCC	4 cut(s)	1463, 1495, 1548, 1580
Alw21I	GWGCWC	1 cut(s)	2768
Alw26I	GTCTC	5 cut(s)	149, 308, 359, 1973, 2263
AlwI	GGATC	5 cut(s)	1049, 2183, 2190, 2203, 2985
AlwNI	CAGNNNCTG	1 cut(s)	2338
Ama87I	CYCGRG	1 cut(s)	284
AoxI	GGCC	8 cut(s)	128, 643, 797, 1020, 1589, 1869, 2017, 2340
ApeKI	GCWGC	6 cut(s)	11, 170, 413, 899, 917, 1601
ApoI	RAATTY	4 cut(s)	948, 1525, 2569, 3162
Asp700I	GAANNNNTTC	4 cut(s)	567, 1343, 1455, 2366
Asp718I	GGTACC	1 cut(s)	2823
AspLEI	GCGC	1 cut(s)	283
AspS9I	GGNCC	6 cut(s)	483, 693, 1021, 1829, 2018, 2341
AsuHPI	GGTGA	5 cut(s)	572, 2198, 2224, 2474, 2702
AvaI	CYCGRG	1 cut(s)	284
AvaII	GGWCC	3 cut(s)	483, 693, 1829
BaeGI	GKGCMC	1 cut(s)	3036
BalI	TGGCCA	2 cut(s)	799, 1871
BamHI	GGATCC	1 cut(s)	2195
BanI	GGYRCC	1 cut(s)	2823
BanII	GRGCYC	1 cut(s)	2768
BauI	CACGAG	1 cut(s)	1758
BbsI	GAAGAC	2 cut(s)	1740, 2102
Bbv12I	GWGCWC	1 cut(s)	2768
BbvCI	CCTCAGC	2 cut(s)	387, 1550
BbvI	GCAGC	5 cut(s)	157, 400, 886, 904, 1588
BccI	CCATC	7 cut(s)	473, 613, 1012, 1778, 1840, 2909, 3059
BceAI	ACGGC	1 cut(s)	1604
BcgI	CGANNNNNNTGC	2 cut(s)	578, 612
BciT130I	CCWGG	1 cut(s)	2193
BcoDI	GTCTC	5 cut(s)	149, 308, 359, 1973, 2263
BcuI	ACTAGT	1 cut(s)	224
BfaI	CTAG	7 cut(s)	225, 888, 1065, 1694, 2666, 2691, 2838
BfmI	CTRYAG	3 cut(s)	333, 700, 3114
BglII	AGATCT	1 cut(s)	2863
BisI	GCNGC	7 cut(s)	12, 171, 414, 537, 900, 918, 1602
BlpI	GCTNAGC	2 cut(s)	3002, 3007
BlsI	GCNGC	7 cut(s)	13, 172, 415, 538, 901, 919, 1603
BmcAI	AGTACT	2 cut(s)	1535, 2366
Bme1390I	CCNGG	1 cut(s)	2193
Bme18I	GGWCC	3 cut(s)	483, 693, 1829
BmeT110I	CYCGRG	1 cut(s)	284
BmgT120I	GGNCC	6 cut(s)	483, 693, 1021, 1829, 2018, 2341
BmiI	GGNNCC	3 cut(s)	484, 2197, 2825
BmrFI	CCNGG	1 cut(s)	2193
BmrI	ACTGGG	1 cut(s)	247
BmsI	GCATC	3 cut(s)	598, 886, 2530
BmuI	ACTGGG	1 cut(s)	247
BpiI	GAAGAC	2 cut(s)	1740, 2102
BpmI	CTGGAG	1 cut(s)	2992
Bpu10I	CCTNAGC	2 cut(s)	387, 1550
Bpu1102I	GCTNAGC	2 cut(s)	3002, 3007
BpuEI	CTTGAG	2 cut(s)	276, 1422
BsaHI	GRCGYC	1 cut(s)	139
BsaJI	CCNNGG	3 cut(s)	531, 1058, 2857
BsaXI	ACNNNNNCTCC	2 cut(s)	1463, 1493
Bsc4I	CCNNNNNNNGG	4 cut(s)	126, 388, 652, 2312
Bse1I	ACTGG	9 cut(s)	242, 620, 1168, 1321, 1367, 1515, 2343, 2494, 2622
Bse3DI	GCAATG	3 cut(s)	165, 486, 866
BseBI	CCWGG	1 cut(s)	2193
BseDI	CCNNGG	3 cut(s)	531, 1058, 2857
BseGI	GGATG	8 cut(s)	624, 678, 1051, 2230, 2554, 2626, 2893, 3140
BseLI	CCNNNNNNNGG	4 cut(s)	126, 388, 652, 2312
BseMI	GCAATG	3 cut(s)	165, 486, 866
BseMII	CTCAG	9 cut(s)	160, 378, 744, 852, 1005, 1185, 1541, 2247, 3021
BseNI	ACTGG	9 cut(s)	242, 620, 1168, 1321, 1367, 1515, 2343, 2494, 2622
BseRI	GAGGAG	3 cut(s)	703, 1190, 2834
BseSI	GKGCMC	1 cut(s)	3036
BseXI	GCAGC	5 cut(s)	157, 400, 886, 904, 1588
BseYI	CCCAGC	1 cut(s)	352
BsgI	GTGCAG	3 cut(s)	470, 885, 2438
BshFI	GGCC	8 cut(s)	130, 645, 799, 1022, 1591, 1871, 2019, 2342
BshNI	GGYRCC	1 cut(s)	2823
BsiHKAI	GWGCWC	1 cut(s)	2768
BsiHKCI	CYCGRG	1 cut(s)	284
BsiSI	CCGG	1 cut(s)	343
BslFI	GGGAC	2 cut(s)	263, 3139
BslI	CCNNNNNNNGG	4 cut(s)	126, 388, 652, 2312
BsmAI	GTCTC	5 cut(s)	149, 308, 359, 1973, 2263
BsmBI	CGTCTC	2 cut(s)	149, 1973
BsmFI	GGGAC	2 cut(s)	263, 3139
BsmI	GAATGC	1 cut(s)	1084
BsnI	GGCC	8 cut(s)	130, 645, 799, 1022, 1591, 1871, 2019, 2342
BsoBI	CYCGRG	1 cut(s)	284
Bsp1286I	GDGCHC	2 cut(s)	2768, 3036
Bsp143I	GATC	7 cut(s)	1054, 1543, 1725, 2188, 2195, 2863, 2977
Bsp1720I	GCTNAGC	2 cut(s)	3002, 3007
BspACI	CCGC	7 cut(s)	97, 259, 467, 536, 2755, 2848, 3011
BspANI	GGCC	8 cut(s)	130, 645, 799, 1022, 1591, 1871, 2019, 2342
BspCNI	CTCAG	9 cut(s)	159, 379, 743, 851, 1006, 1186, 1542, 2248, 3020
BspHI	TCATGA	2 cut(s)	475, 2701
BspLI	GGNNCC	3 cut(s)	484, 2197, 2825
BspMAI	CTGCAG	1 cut(s)	337
BspPI	GGATC	5 cut(s)	1049, 2183, 2190, 2203, 2985
BspQI	GCTCTTC	4 cut(s)	2164, 2378, 2756, 2773
BspT107I	GGYRCC	1 cut(s)	2823
BsrDI	GCAATG	3 cut(s)	165, 486, 866
BsrI	ACTGG	9 cut(s)	242, 620, 1168, 1321, 1367, 1515, 2343, 2494, 2622
BssECI	CCNNGG	3 cut(s)	531, 1058, 2857
BssMI	GATC	7 cut(s)	1054, 1543, 1725, 2188, 2195, 2863, 2977
BssNI	GRCGYC	1 cut(s)	139
BssSI	CACGAG	1 cut(s)	1758
BssT1I	CCWWGG	3 cut(s)	531, 1058, 2857
Bst2BI	CACGAG	1 cut(s)	1758
Bst2UI	CCWGG	1 cut(s)	2193
Bst6I	CTCTTC	7 cut(s)	678, 1175, 1329, 2164, 2378, 2756, 2773
BstACI	GRCGYC	1 cut(s)	139
BstC8I	GCNNGC	8 cut(s)	97, 904, 1672, 1716, 2219, 2505, 2783, 2787
BstEII	GGTNACC	1 cut(s)	577
BstF5I	GGATG	8 cut(s)	624, 678, 1051, 2230, 2554, 2626, 2893, 3140
BstHHI	GCGC	1 cut(s)	283
BstKTI	GATC	7 cut(s)	1057, 1546, 1728, 2191, 2198, 2866, 2980
BstMAI	GTCTC	5 cut(s)	149, 308, 359, 1973, 2263
BstMBI	GATC	7 cut(s)	1054, 1543, 1725, 2188, 2195, 2863, 2977
BstNI	CCWGG	1 cut(s)	2193
BstNSI	RCATGY	4 cut(s)	1480, 2432, 2503, 2924
BstPI	GGTNACC	1 cut(s)	577
BstSCI	CCNGG	1 cut(s)	2191
BstSFI	CTRYAG	3 cut(s)	333, 700, 3114
BstSLI	GKGCMC	1 cut(s)	3036
BstV1I	GCAGC	5 cut(s)	157, 400, 886, 904, 1588
BstV2I	GAAGAC	2 cut(s)	1740, 2102
BstX2I	RGATCY	2 cut(s)	2195, 2863
BstXI	CCANNNNNNTGG	2 cut(s)	2165, 2565
BstYI	RGATCY	2 cut(s)	2195, 2863
BsuRI	GGCC	8 cut(s)	130, 645, 799, 1022, 1591, 1871, 2019, 2342
BtgZI	GCGATG	1 cut(s)	617
BtsCI	GGATG	8 cut(s)	624, 678, 1051, 2230, 2554, 2626, 2893, 3140
BtsI	GCAGTG	1 cut(s)	2514
BtsIMutI	CAGTG	8 cut(s)	781, 1522, 1680, 1947, 2068, 2253, 2514, 2543
Cac8I	GCNNGC	8 cut(s)	97, 904, 1672, 1716, 2219, 2505, 2783, 2787
CaiI	CAGNNNCTG	1 cut(s)	2338
CciI	TCATGA	2 cut(s)	475, 2701
CfoI	GCGC	1 cut(s)	283
Cfr13I	GGNCC	6 cut(s)	483, 693, 1021, 1829, 2018, 2341
CseI	GACGC	2 cut(s)	425, 1990
Csp6I	GTAC	9 cut(s)	296, 1474, 1534, 2008, 2066, 2365, 2497, 2552, 2824
CspCI	CAANNNNNGTGG	2 cut(s)	1427, 1462
CviQI	GTAC	9 cut(s)	296, 1474, 1534, 2008, 2066, 2365, 2497, 2552, 2824
DpnI	GATC	7 cut(s)	1056, 1545, 1727, 2190, 2197, 2865, 2979
DpnII	GATC	7 cut(s)	1054, 1543, 1725, 2188, 2195, 2863, 2977
DraIII	CACNNNGTG	1 cut(s)	2259
EaeI	YGGCCR	2 cut(s)	797, 1869
Eam1104I	CTCTTC	7 cut(s)	678, 1175, 1329, 2164, 2378, 2756, 2773
EarI	CTCTTC	7 cut(s)	678, 1175, 1329, 2164, 2378, 2756, 2773
Ecl136II	GAGCTC	1 cut(s)	2766
Eco130I	CCWWGG	3 cut(s)	531, 1058, 2857
Eco147I	AGGCCT	1 cut(s)	645
Eco24I	GRGCYC	1 cut(s)	2768
Eco32I	GATATC	2 cut(s)	379, 1932
Eco47I	GGWCC	3 cut(s)	483, 693, 1829
Eco53kI	GAGCTC	1 cut(s)	2766
Eco57I	CTGAAG	6 cut(s)	702, 1014, 2072, 2109, 2567, 2728
Eco88I	CYCGRG	1 cut(s)	284
Eco91I	GGTNACC	1 cut(s)	577
EcoICRI	GAGCTC	1 cut(s)	2766
EcoO65I	GGTNACC	1 cut(s)	577
EcoRI	GAATTC	2 cut(s)	948, 1525
EcoRII	CCWGG	1 cut(s)	2191
EcoRV	GATATC	2 cut(s)	379, 1932
EcoT14I	CCWWGG	3 cut(s)	531, 1058, 2857
EcoT38I	GRGCYC	1 cut(s)	2768
ErhI	CCWWGG	3 cut(s)	531, 1058, 2857
Esp3I	CGTCTC	2 cut(s)	149, 1973
FalI	AAGNNNNNCTT	6 cut(s)	173, 205, 193, 225, 2354, 2386
FaqI	GGGAC	2 cut(s)	263, 3139
FauI	CCCGC	1 cut(s)	104
Fnu4HI	GCNGC	7 cut(s)	12, 171, 414, 537, 900, 918, 1602
FokI	GGATG	8 cut(s)	631, 685, 1058, 2237, 2541, 2633, 2900, 3127
FriOI	GRGCYC	1 cut(s)	2768
Fsp4HI	GCNGC	7 cut(s)	12, 171, 414, 537, 900, 918, 1602
FspBI	CTAG	7 cut(s)	225, 888, 1065, 1694, 2666, 2691, 2838
GlaI	GCGC	1 cut(s)	282
GluI	GCNGC	7 cut(s)	12, 171, 414, 537, 900, 918, 1602
GsaI	CCCAGC	1 cut(s)	356
GsuI	CTGGAG	1 cut(s)	2992
HaeIII	GGCC	8 cut(s)	130, 645, 799, 1022, 1591, 1871, 2019, 2342
HapII	CCGG	1 cut(s)	343
HgaI	GACGC	2 cut(s)	425, 1990
HhaI	GCGC	1 cut(s)	283
Hin1I	GRCGYC	1 cut(s)	139
Hin6I	GCGC	1 cut(s)	281
HinP1I	GCGC	1 cut(s)	281
HincII	GTYRAC	3 cut(s)	835, 990, 3111
HindII	GTYRAC	3 cut(s)	835, 990, 3111
HindIII	AAGCTT	3 cut(s)	425, 1422, 2579
HpaII	CCGG	1 cut(s)	343
HphI	GGTGA	5 cut(s)	572, 2198, 2224, 2474, 2702
Hpy166II	GTNNAC	9 cut(s)	586, 779, 835, 990, 2068, 2710, 2989, 3016, 3111
Hpy8I	GTNNAC	9 cut(s)	586, 779, 835, 990, 2068, 2710, 2989, 3016, 3111
Hpy99I	CGWCG	2 cut(s)	141, 144
HpyCH4IV	ACGT	5 cut(s)	139, 518, 2535, 2706, 3018
HpySE526I	ACGT	5 cut(s)	139, 518, 2535, 2706, 3018
Hsp92I	GRCGYC	1 cut(s)	139
HspAI	GCGC	1 cut(s)	281
KpnI	GGTACC	1 cut(s)	2827
Kzo9I	GATC	7 cut(s)	1054, 1543, 1725, 2188, 2195, 2863, 2977
LguI	GCTCTTC	4 cut(s)	2164, 2378, 2756, 2773
LmnI	GCTCC	1 cut(s)	1771
Lsp1109I	GCAGC	5 cut(s)	157, 400, 886, 904, 1588
LweI	GCATC	3 cut(s)	598, 886, 2530
MaeI	CTAG	7 cut(s)	225, 888, 1065, 1694, 2666, 2691, 2838
MaeII	ACGT	5 cut(s)	139, 518, 2535, 2706, 3018
MaeIII	GTNAC	4 cut(s)	220, 577, 707, 2139
MalI	GATC	7 cut(s)	1056, 1545, 1727, 2190, 2197, 2865, 2979
MboI	GATC	7 cut(s)	1054, 1543, 1725, 2188, 2195, 2863, 2977
MfeI	CAATTG	3 cut(s)	2025, 2307, 2322
MflI	RGATCY	2 cut(s)	2195, 2863
MhlI	GDGCHC	2 cut(s)	2768, 3036
MlsI	TGGCCA	2 cut(s)	799, 1871
MluNI	TGGCCA	2 cut(s)	799, 1871
MlyI	GAGTC	7 cut(s)	310, 830, 1946, 1960, 2147, 2666, 3161
MmeI	TCCRAC	2 cut(s)	720, 1486
Mox20I	TGGCCA	2 cut(s)	799, 1871
MroXI	GAANNNNTTC	4 cut(s)	567, 1343, 1455, 2366
MscI	TGGCCA	2 cut(s)	799, 1871
MslI	CAYNNNNRTG	5 cut(s)	444, 957, 1376, 2433, 3086
Msp20I	TGGCCA	2 cut(s)	799, 1871
MspA1I	CMGCKG	2 cut(s)	680, 3011
MspI	CCGG	1 cut(s)	343
MspR9I	CCNGG	1 cut(s)	2193
MunI	CAATTG	3 cut(s)	2025, 2307, 2322
Mva1269I	GAATGC	1 cut(s)	1084
MvaI	CCWGG	1 cut(s)	2193
NdeII	GATC	7 cut(s)	1054, 1543, 1725, 2188, 2195, 2863, 2977
NlaIV	GGNNCC	3 cut(s)	484, 2197, 2825
NmeAIII	GCCGAG	1 cut(s)	106
NmuCI	GTSAC	2 cut(s)	707, 2139
NspI	RCATGY	4 cut(s)	1480, 2432, 2503, 2924
PaeR7I	CTCGAG	1 cut(s)	284
PagI	TCATGA	2 cut(s)	475, 2701
PceI	AGGCCT	1 cut(s)	645
PciI	ACATGT	2 cut(s)	1476, 2920
PciSI	GCTCTTC	4 cut(s)	2164, 2378, 2756, 2773
PcsI	WCGNNNNNNNCGW	1 cut(s)	133
PctI	GAATGC	1 cut(s)	1084
PdmI	GAANNNNTTC	4 cut(s)	567, 1343, 1455, 2366
PfeI	GAWTC	8 cut(s)	1201, 1237, 1247, 2624, 2751, 2900, 3040, 3119
PflFI	GACNNNGTC	1 cut(s)	141
PfoI	TCCNGGA	1 cut(s)	2191
PkrI	GCNGC	7 cut(s)	13, 172, 415, 538, 901, 919, 1603
PleI	GAGTC	7 cut(s)	309, 830, 1945, 1959, 2146, 2666, 3160
PpsI	GAGTC	7 cut(s)	309, 830, 1945, 1959, 2146, 2666, 3160
PscI	ACATGT	2 cut(s)	1476, 2920
PsiI	TTATAA	1 cut(s)	200
Psp124BI	GAGCTC	1 cut(s)	2768
Psp1406I	AACGTT	2 cut(s)	518, 2535
Psp6I	CCWGG	1 cut(s)	2191
PspEI	GGTNACC	1 cut(s)	577
PspFI	CCCAGC	1 cut(s)	352
PspGI	CCWGG	1 cut(s)	2191
PspN4I	GGNNCC	3 cut(s)	484, 2197, 2825
PspPI	GGNCC	6 cut(s)	483, 693, 1021, 1829, 2018, 2341
PspXI	VCTCGAGB	1 cut(s)	284
PstI	CTGCAG	1 cut(s)	337
PstNI	CAGNNNCTG	1 cut(s)	2338
PsuI	RGATCY	2 cut(s)	2195, 2863
PsyI	GACNNNGTC	1 cut(s)	141
PvuII	CAGCTG	1 cut(s)	680
RsaI	GTAC	9 cut(s)	297, 1475, 1535, 2009, 2067, 2366, 2498, 2553, 2825
RsaNI	GTAC	9 cut(s)	296, 1474, 1534, 2008, 2066, 2365, 2497, 2552, 2824
RseI	CAYNNNNRTG	5 cut(s)	444, 957, 1376, 2433, 3086
SacI	GAGCTC	1 cut(s)	2768
SapI	GCTCTTC	4 cut(s)	2164, 2378, 2756, 2773
SatI	GCNGC	7 cut(s)	12, 171, 414, 537, 900, 918, 1602
Sau3AI	GATC	7 cut(s)	1054, 1543, 1725, 2188, 2195, 2863, 2977
Sau96I	GGNCC	6 cut(s)	483, 693, 1021, 1829, 2018, 2341
ScaI	AGTACT	2 cut(s)	1535, 2366
SchI	GAGTC	7 cut(s)	310, 830, 1946, 1960, 2147, 2666, 3161
ScrFI	CCNGG	1 cut(s)	2193
SduI	GDGCHC	2 cut(s)	2768, 3036
SfaNI	GCATC	3 cut(s)	598, 886, 2530
SfcI	CTRYAG	3 cut(s)	333, 700, 3114
Sfr274I	CTCGAG	1 cut(s)	284
SinI	GGWCC	3 cut(s)	483, 693, 1829
SlaI	CTCGAG	1 cut(s)	284
SmiMI	CAYNNNNRTG	5 cut(s)	444, 957, 1376, 2433, 3086
SmlI	CTYRAG	3 cut(s)	284, 291, 1437
SmoI	CTYRAG	3 cut(s)	284, 291, 1437
SpeI	ACTAGT	1 cut(s)	224
SseBI	AGGCCT	1 cut(s)	645
SsiI	CCGC	7 cut(s)	97, 259, 467, 536, 2755, 2848, 3011
SspI	AATATT	3 cut(s)	1576, 2698, 3134
SspMI	CTAG	7 cut(s)	225, 888, 1065, 1694, 2666, 2691, 2838
SstI	GAGCTC	1 cut(s)	2768
StuI	AGGCCT	1 cut(s)	645
StyD4I	CCNGG	1 cut(s)	2191
StyI	CCWWGG	3 cut(s)	531, 1058, 2857
TaiI	ACGT	5 cut(s)	142, 521, 2538, 2709, 3021
TaqI	TCGA	4 cut(s)	285, 444, 1459, 3073
TaqII	GACCGA	1 cut(s)	1817
TatI	WGTACW	5 cut(s)	295, 1473, 1533, 2364, 2496
TauI	GCSGC	1 cut(s)	539
TfiI	GAWTC	8 cut(s)	1201, 1237, 1247, 2624, 2751, 2900, 3040, 3119
TscAI	CASTG	8 cut(s)	781, 1522, 1687, 1947, 2075, 2260, 2514, 2550
TseFI	GTSAC	2 cut(s)	707, 2139
TseI	GCWGC	6 cut(s)	11, 170, 413, 899, 917, 1601
Tsp45I	GTSAC	2 cut(s)	707, 2139
TspGWI	ACGGA	2 cut(s)	67, 2158
TspRI	CASTG	8 cut(s)	781, 1522, 1687, 1947, 2075, 2260, 2514, 2550
Tth111I	GACNNNGTC	1 cut(s)	141
VpaK11BI	GGWCC	3 cut(s)	483, 693, 1829
XapI	RAATTY	4 cut(s)	948, 1525, 2569, 3162
XbaI	TCTAGA	1 cut(s)	2690
XceI	RCATGY	4 cut(s)	1480, 2432, 2503, 2924
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2206
XhoI	CTCGAG	1 cut(s)	284
XmnI	GAANNNNTTC	4 cut(s)	567, 1343, 1455, 2366
XspI	CTAG	7 cut(s)	225, 888, 1065, 1694, 2666, 2691, 2838
ZraI	GACGTC	1 cut(s)	140
ZrmI	AGTACT	2 cut(s)	1535, 2366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.