Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001741.1

Orthogroup ID

OG0013983

Physical Location & Seq

Genomic Coordinates Reverse (-)

98044 .. 107159

Gene Structure 9116 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0001741.1_g000019.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3420 bp

atggcctctactactactactactagtctccaaatccccactttcctctccctcaaaccactccctcaacgcccctccttccccttctccaaccaccccacctccttctcttccttctccacctcctcctcccgccctccacgccgtcgtctcactctctctcccgcccccattgccgcctctctcaaggagaatctgggccggattggaaagacttggaacgacgtgactagcttgaacagctgggttgttcgagactactaccgccttgtcaactctgtcaactcttttgagccacggctacagaggctcactgatgaccagcttactgctaaaactgccgagtttcggcgaagattgggacaaggagagacacttgcggatattcaagccgaggcttttgctgttgttcgtgaagctgcgaaacggaagcttggtatgcgtcattttgatgttcagattattggtggggcggtgcttcatgatgggtccattgcagaaatgaagacgggagagggaaaaacgttggtttccacattagcagcatatcttaatgccctgactggcgatggtgttcatgtggtaaccgtaaatgattaccttgctcaaagggatgctgagtggatgggtcgtgttcatcgcttcctaggtctttcagtgggtcttattcagagagggatgacagctgacaagaggaggttgaactatagatgtgacataacatacaccaataattcagaacttgggtttgactatctacgagacaatcttgccggaaacagtggagaacttgttatgagatcgccaaagccatttcattttgcaatagttgatgaagttgactcggttcttattgatgaaggaagaaatccattgctaattagtggcgaggctagtaaagatgctgctcgctatcctgttgctgctaaagtggctgagttgcttgtgcgaggaattcattacaatgtagaacttaaagattactcagttgagttgactgaagaaggaatagctcttgctgagatggctcttgaaacaaatgacttatgggatgaaaatgatccctgggccagatttgtgatgaatgcgttgaaagctaaagagttctatcggaaggatgtgcaatatattgtcagaaatgggaaggctctcataatcaatgagctcactggcagagtcgaagataagagaaggtggtctgagggtattcatcaagctgtggagggaaaagaaggtttgaagattcaggctgattcggttgttatcgcacaaatcacatatcaatctctgttcaagctctacccaaagctatcagggatgactggcactgcaaaaactgaagagaaagagttcttgaaaatgttccaaacaccagttattgaagtgccaacaaatttgccaaacattcgtaatgatttacctattcaagcttttgcgactgctcaggggaaatgggaatatgttcgtcaagaagtggaatacatgttcaggcaggggcgtcctgttttggttggcaccaccagtgttgagtattctgaacacctgtctgatctgttgagggaacataacatcccacacaatgttctaaatgcacggcccaagtatgctgcaagggaggctgaaattgttgcccaagctggacgaaaacatgccattactctttctactaatatggctggcagaggcactgacataatcctaggggggaatcctaaaatgcttgcaaaagaaatcatagaagacagtttgatttcattcctgacacgagaagctcctaacattgacatagatggagaggcgatttcacaaaaggtattgtcaaagattaaggtcggtccatcatcattagctttgctggctaagacagcgttaatggctaaatatgttggcaaaaatgaagggaagagctggacatataaggaggcaaaatccatgatatcagagtctgtggaaatgagtcaatcaagagatttgaaagagctagagaggcttgttgatgagcagtctgagatttaccctcttggccctacaatagcacttgcttatctgtcagttttgaaggattgcgaggtacactgtttcaaagaagggtctgaagtgaaaagacttgggggacttcatgtgattggaacatctttgcacgagtctcgtagaatagataaccagcttcgtggcagagcaggaaggcaaggtgatccaggatcaactcgatttatggtgagcttgcaggatgagatgtttcaaaagtttaattttgatactgagtgggctgtgagacttatttcaaaaattactgatgatgaggatgtaccaattgaaggtgatgccattgtgagacagctcctgtccctgcaaattaatgctgaaaagtacttttttggcataagaaagagcctggttgagtttgatgaagtattagaggtgcagagaaagcatgtttatgaacttcggcagtcaattttaactggtgataatgaaagttgttcccagcatgtattccagtacatgcaagcagtggtagatgaaattgtctttaaaaatgttgacaccctcaagcatccaagaaaatggagtttaaataagcttttgaaggagtttatggcaattgctgggaaactattggatgattcatttgcaggaattactgaggaagctttgttagaatctcttgcgcagtcacatgaactgaactttaaagatattgatgacattcaccttccaaacttgccaaaacctccaaatgccttcagaggtatccgcaagaagaactcttcattgaaacgttggcttgctatatgctcggatgatttgaccaagaatggaaggtaccatgctactactaacctcctccgcaagtaccttggagactatttgattgcttcatatttggatgttgttcaagactctggttatgatgatacatatgtgaaggaagttgaaagagcagttattgtgaaaacccttgattgtttctggagggatcaccttgtaaacatgaacaggctaagttcagcggtgaatgtaaggagttttgggcacaggaatcctcttgaagaatacaagattgatggttgtcgttttttcatatctatgcttagtgcaacacgaaggttgactgtagagtcacttttgcaatattggtcctccccaatggagtctcaagaaatttttgaaccatggtttgtatcttggtgggctagtatgtcaaccaaactgatcttgcctggtatgaagtgtgttgctcttatccctcagcttcattatatgaagagtattgaacttgtaaccttttccataggattggacccgagaagtgcactgtttcagtgtttcattgaagaaggcagcaggtttttgctgaggaacagaggaattggaactgcaatgaacaccttgattggagtggagcaaactaattga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0006996 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0009657 GO:0009658 GO:0009987 GO:0010109 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019222 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0031323 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW WD40 zf-RING_UBOX

Protein Analysis

1139

Amino Acids

128.72

Weight (kDa)

6.82

Isoelectric Point (pI)

38.33

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21650 AT1G21650 AT1G21650
fragaria_vesca	FvH4_2g27750
malus_domestica	MD00G1100600.v1.1
prunus_persica	Prupe.1G490400_v2.0.a1
pyrus_communis	pycom15g30570
rosa_chinensis	RchiOBHm_Chr6g0296621
rosa_laevigata	RLG00000011676
rosa_multiflora	Rmu_sc0001741.1_g000019
rosa_roxburghii	Rroxscaffold_7G00171330 Rroxscaffold_7G00178770
rosa_rugosa	Rorug06G0263700
rosa_samantha	Rh6AG375100 Rh6CG388700
rosa_wichuraiana	Rw6G032720 Rw6G032810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3121
Acc16I	TGCGCA	1 cut(s)	2673
Acc36I	ACCTGC	1 cut(s)	3339
Acc65I	GGTACC	1 cut(s)	2826
AccB1I	GGYRCC	2 cut(s)	1514, 2826
AciI	CCGC	9 cut(s)	133, 165, 177, 265, 380, 473, 2758, 2851, 3014
AclI	AACGTT	2 cut(s)	524, 2782
AclWI	GGATC	4 cut(s)	1055, 2189, 2209, 2988
AcsI	RAATTY	3 cut(s)	954, 1393, 3165
AcuI	CTGAAG	4 cut(s)	1020, 1359, 2115, 2731
AcyI	GRCGYC	1 cut(s)	1498
AfaI	GTAC	6 cut(s)	2073, 2310, 2372, 2504, 2828, 2858
AfiI	CCNNNNNNNGG	2 cut(s)	348, 2318
AflIII	ACRYGT	1 cut(s)	1482
AhlI	ACTAGT	1 cut(s)	23
AjiI	CACGTC	1 cut(s)	226
AjnI	CCWGG	4 cut(s)	1064, 2197, 2394, 3223
AloI	GAACNNNNNNTCC	4 cut(s)	1469, 1501, 1554, 1586
Alw21I	GWGCWC	2 cut(s)	1167, 3319
Alw44I	GTGCAC	1 cut(s)	3315
AlwI	GGATC	4 cut(s)	1055, 2189, 2209, 2988
AlwNI	CAGNNNCTG	2 cut(s)	1946, 2344
Ama87I	CYCGRG	1 cut(s)	3307
AoxI	GGCC	5 cut(s)	3, 199, 1068, 1595, 2023
ApaLI	GTGCAC	1 cut(s)	3315
ApeKI	GCWGC	6 cut(s)	419, 542, 905, 923, 1607, 3345
ApoI	RAATTY	3 cut(s)	954, 1393, 3165
AseI	ATTAAT	1 cut(s)	2358
Asp700I	GAANNNNTTC	3 cut(s)	1349, 1361, 1461
Asp718I	GGTACC	1 cut(s)	2826
AspA2I	CCTAGG	2 cut(s)	646, 1699
AspLEI	GCGC	1 cut(s)	2674
AspS9I	GGNCC	8 cut(s)	199, 489, 1068, 1596, 1835, 2024, 3141, 3304
AsuHPI	GGTGA	7 cut(s)	2204, 2230, 2333, 2480, 2705, 2975, 3028
AvaI	CYCGRG	1 cut(s)	3307
AvaII	GGWCC	4 cut(s)	489, 1835, 3141, 3304
AvrII	CCTAGG	2 cut(s)	646, 1699
BaeGI	GKGCMC	2 cut(s)	3039, 3319
BaeI	ACNNNNGTAYC	2 cut(s)	2910, 2943
BanI	GGYRCC	2 cut(s)	1514, 2826
BanII	GRGCYC	1 cut(s)	1167
BauI	CACGAG	2 cut(s)	1764, 2141
BbsI	GAAGAC	2 cut(s)	512, 1746
Bbv12I	GWGCWC	2 cut(s)	1167, 3319
BbvCI	CCTCAGC	2 cut(s)	3252, 3359
BbvI	GCAGC	6 cut(s)	406, 554, 892, 910, 1594, 3357
BccI	CCATC	7 cut(s)	479, 563, 619, 1018, 1784, 1846, 3062
BceAI	ACGGC	3 cut(s)	129, 314, 1610
BcgI	CGANNNNNNTGC	4 cut(s)	383, 417, 2129, 2163
BciT130I	CCWGG	4 cut(s)	1066, 2199, 2396, 3225
BciVI	GTATCC	1 cut(s)	2765
BcuI	ACTAGT	1 cut(s)	23
BfaI	CTAG	7 cut(s)	24, 231, 647, 894, 1700, 1982, 3198
BfmI	CTRYAG	3 cut(s)	302, 706, 3117
BfuAI	ACCTGC	1 cut(s)	3339
BfuI	GTATCC	1 cut(s)	2765
BisI	GCNGC	7 cut(s)	177, 420, 543, 906, 924, 1608, 3346
BlnI	CCTAGG	2 cut(s)	646, 1699
BlsI	GCNGC	7 cut(s)	178, 421, 544, 907, 925, 1609, 3347
BmcAI	AGTACT	1 cut(s)	2372
Bme1390I	CCNGG	4 cut(s)	1066, 2199, 2396, 3225
Bme18I	GGWCC	4 cut(s)	489, 1835, 3141, 3304
BmeT110I	CYCGRG	1 cut(s)	3307
BmgBI	CACGTC	1 cut(s)	226
BmgT120I	GGNCC	8 cut(s)	199, 489, 1068, 1596, 1835, 2024, 3141, 3304
BmiI	GGNNCC	4 cut(s)	490, 1516, 2828, 3306
BmrFI	CCNGG	4 cut(s)	1066, 2199, 2396, 3225
BmsI	GCATC	4 cut(s)	604, 892, 2314, 2566
BpiI	GAAGAC	2 cut(s)	512, 1746
BpmI	CTGGAG	1 cut(s)	2995
Bpu10I	CCTNAGC	3 cut(s)	1443, 3252, 3359
BpuEI	CTTGAG	3 cut(s)	170, 2537, 3144
BsaHI	GRCGYC	1 cut(s)	1498
BsaJI	CCNNGG	8 cut(s)	296, 393, 646, 1064, 1065, 1699, 2860, 3176
BsaXI	ACNNNNNCTCC	6 cut(s)	86, 116, 145, 175, 777, 807
Bsc4I	CCNNNNNNNGG	2 cut(s)	348, 2318
Bse1I	ACTGG	7 cut(s)	568, 1174, 1327, 1373, 1521, 2470, 2500
Bse3DI	GCAATG	4 cut(s)	171, 492, 872, 3390
BseBI	CCWGG	4 cut(s)	1066, 2199, 2396, 3225
BseDI	CCNNGG	8 cut(s)	296, 393, 646, 1064, 1065, 1699, 2860, 3176
BseLI	CCNNNNNNNGG	2 cut(s)	348, 2318
BseMI	GCAATG	4 cut(s)	171, 492, 872, 3390
BseNI	ACTGG	7 cut(s)	568, 1174, 1327, 1373, 1521, 2470, 2500
BseRI	GAGGAG	4 cut(s)	115, 118, 709, 2837
BseSI	GKGCMC	2 cut(s)	3039, 3319
BseXI	GCAGC	6 cut(s)	406, 554, 892, 910, 1594, 3357
BseYI	CCCAGC	3 cut(s)	243, 2487, 2609
BsgI	GTGCAG	1 cut(s)	2444
BshFI	GGCC	5 cut(s)	5, 201, 1070, 1597, 2025
BshNI	GGYRCC	2 cut(s)	1514, 2826
BsiHKAI	GWGCWC	2 cut(s)	1167, 3319
BsiHKCI	CYCGRG	1 cut(s)	3307
BsiSI	CCGG	2 cut(s)	202, 774
BslFI	GGGAC	3 cut(s)	375, 2127, 2332
BslI	CCNNNNNNNGG	2 cut(s)	348, 2318
BsmBI	CGTCTC	1 cut(s)	155
BsmFI	GGGAC	3 cut(s)	375, 2127, 2332
BsmI	GAATGC	1 cut(s)	1090
BsnI	GGCC	5 cut(s)	5, 201, 1070, 1597, 2025
BsoBI	CYCGRG	1 cut(s)	3307
Bsp1286I	GDGCHC	3 cut(s)	1167, 3039, 3319
Bsp143I	GATC	7 cut(s)	800, 1060, 1549, 2194, 2201, 2980, 3216
Bsp19I	CCATGG	1 cut(s)	3176
BspACI	CCGC	9 cut(s)	133, 165, 177, 265, 380, 473, 2758, 2851, 3014
BspANI	GGCC	5 cut(s)	5, 201, 1070, 1597, 2025
BspHI	TCATGA	1 cut(s)	481
BspLI	GGNNCC	4 cut(s)	490, 1516, 2828, 3306
BspMI	ACCTGC	1 cut(s)	3339
BspPI	GGATC	4 cut(s)	1055, 2189, 2209, 2988
BspQI	GCTCTTC	1 cut(s)	1898
BspT107I	GGYRCC	2 cut(s)	1514, 2826
BsrDI	GCAATG	4 cut(s)	171, 492, 872, 3390
BsrI	ACTGG	7 cut(s)	568, 1174, 1327, 1373, 1521, 2470, 2500
BssECI	CCNNGG	8 cut(s)	296, 393, 646, 1064, 1065, 1699, 2860, 3176
BssMI	GATC	7 cut(s)	800, 1060, 1549, 2194, 2201, 2980, 3216
BssNI	GRCGYC	1 cut(s)	1498
BssSI	CACGAG	2 cut(s)	1764, 2141
BssT1I	CCWWGG	4 cut(s)	646, 1699, 2860, 3176
Bst2BI	CACGAG	2 cut(s)	1764, 2141
Bst2UI	CCWGG	4 cut(s)	1066, 2199, 2396, 3225
Bst4CI	ACNGT	6 cut(s)	589, 782, 1745, 2078, 3118, 3321
Bst6I	CTCTTC	5 cut(s)	115, 1335, 1898, 2776, 3263
BstACI	GRCGYC	1 cut(s)	1498
BstC8I	GCNNGC	7 cut(s)	910, 1678, 1722, 1857, 2225, 2511, 2790
BstDSI	CCRYGG	2 cut(s)	296, 3176
BstEII	GGTNACC	1 cut(s)	583
BstHHI	GCGC	1 cut(s)	2674
BstKTI	GATC	7 cut(s)	803, 1063, 1552, 2197, 2204, 2983, 3219
BstMBI	GATC	7 cut(s)	800, 1060, 1549, 2194, 2201, 2980, 3216
BstNI	CCWGG	4 cut(s)	1066, 2199, 2396, 3225
BstNSI	RCATGY	5 cut(s)	1486, 1652, 2438, 2495, 2509
BstPI	GGTNACC	1 cut(s)	583
BstSCI	CCNGG	4 cut(s)	1064, 2197, 2394, 3223
BstSFI	CTRYAG	3 cut(s)	302, 706, 3117
BstSLI	GKGCMC	2 cut(s)	3039, 3319
BstV1I	GCAGC	6 cut(s)	406, 554, 892, 910, 1594, 3357
BstV2I	GAAGAC	2 cut(s)	512, 1746
BstXI	CCANNNNNNTGG	3 cut(s)	2171, 2568, 3301
BsuI	GTATCC	1 cut(s)	2765
BsuRI	GGCC	5 cut(s)	5, 201, 1070, 1597, 2025
BtgI	CCRYGG	2 cut(s)	296, 3176
BtgZI	GCGATG	2 cut(s)	582, 623
BtrI	CACGTC	1 cut(s)	226
BtsI	GCAGTG	2 cut(s)	1326, 2520
BveI	ACCTGC	1 cut(s)	3339
Cac8I	GCNNGC	7 cut(s)	910, 1678, 1722, 1857, 2225, 2511, 2790
CaiI	CAGNNNCTG	2 cut(s)	1946, 2344
CciI	TCATGA	1 cut(s)	481
CfoI	GCGC	1 cut(s)	2674
Cfr13I	GGNCC	8 cut(s)	199, 489, 1068, 1596, 1835, 2024, 3141, 3304
CseI	GACGC	2 cut(s)	431, 1487
Csp6I	GTAC	6 cut(s)	2072, 2309, 2371, 2503, 2827, 2857
CspCI	CAANNNNNGTGG	2 cut(s)	1507, 1542
CviQI	GTAC	6 cut(s)	2072, 2309, 2371, 2503, 2827, 2857
DpnI	GATC	7 cut(s)	802, 1062, 1551, 2196, 2203, 2982, 3218
DpnII	GATC	7 cut(s)	800, 1060, 1549, 2194, 2201, 2980, 3216
DraI	TTTAAA	3 cut(s)	2536, 2577, 2695
DrdI	GACNNNNNNGTC	1 cut(s)	3121
DseDI	GACNNNNNNGTC	1 cut(s)	3121
Eam1104I	CTCTTC	5 cut(s)	115, 1335, 1898, 2776, 3263
EarI	CTCTTC	5 cut(s)	115, 1335, 1898, 2776, 3263
Ecl136II	GAGCTC	1 cut(s)	1165
Eco130I	CCWWGG	4 cut(s)	646, 1699, 2860, 3176
Eco24I	GRGCYC	1 cut(s)	1167
Eco32I	GATATC	1 cut(s)	1938
Eco47I	GGWCC	4 cut(s)	489, 1835, 3141, 3304
Eco53kI	GAGCTC	1 cut(s)	1165
Eco57I	CTGAAG	4 cut(s)	1020, 1359, 2115, 2731
Eco88I	CYCGRG	1 cut(s)	3307
Eco91I	GGTNACC	1 cut(s)	583
EcoICRI	GAGCTC	1 cut(s)	1165
EcoO65I	GGTNACC	1 cut(s)	583
EcoRI	GAATTC	1 cut(s)	954
EcoRII	CCWGG	4 cut(s)	1064, 2197, 2394, 3223
EcoRV	GATATC	1 cut(s)	1938
EcoT14I	CCWWGG	4 cut(s)	646, 1699, 2860, 3176
EcoT38I	GRGCYC	1 cut(s)	1167
ErhI	CCWWGG	4 cut(s)	646, 1699, 2860, 3176
Esp3I	CGTCTC	1 cut(s)	155
FaqI	GGGAC	3 cut(s)	375, 2127, 2332
FauI	CCCGC	2 cut(s)	140, 172
FauNDI	CATATG	1 cut(s)	2923
Fnu4HI	GCNGC	7 cut(s)	177, 420, 543, 906, 924, 1608, 3346
FriOI	GRGCYC	1 cut(s)	1167
Fsp4HI	GCNGC	7 cut(s)	177, 420, 543, 906, 924, 1608, 3346
FspBI	CTAG	7 cut(s)	24, 231, 647, 894, 1700, 1982, 3198
FspI	TGCGCA	1 cut(s)	2673
GlaI	GCGC	1 cut(s)	2673
GluI	GCNGC	7 cut(s)	177, 420, 543, 906, 924, 1608, 3346
GsaI	CCCAGC	3 cut(s)	247, 2491, 2613
GsuI	CTGGAG	1 cut(s)	2995
HaeIII	GGCC	5 cut(s)	5, 201, 1070, 1597, 2025
HapII	CCGG	2 cut(s)	202, 774
HgaI	GACGC	2 cut(s)	431, 1487
HhaI	GCGC	1 cut(s)	2674
Hin1I	GRCGYC	1 cut(s)	1498
Hin6I	GCGC	1 cut(s)	2672
HinP1I	GCGC	1 cut(s)	2672
HincII	GTYRAC	7 cut(s)	274, 283, 841, 996, 2545, 3114, 3207
HindII	GTYRAC	7 cut(s)	274, 283, 841, 996, 2545, 3114, 3207
HindIII	AAGCTT	4 cut(s)	431, 1428, 2582, 2652
HpaII	CCGG	2 cut(s)	202, 774
HphI	GGTGA	7 cut(s)	2204, 2230, 2333, 2480, 2705, 2975, 3028
Hpy99I	CGWCG	2 cut(s)	150, 227
HpyCH4III	ACNGT	6 cut(s)	589, 782, 1745, 2078, 3118, 3321
HpyCH4IV	ACGT	3 cut(s)	225, 524, 2782
HpySE526I	ACGT	3 cut(s)	225, 524, 2782
Hsp92I	GRCGYC	1 cut(s)	1498
HspAI	GCGC	1 cut(s)	2672
KpnI	GGTACC	1 cut(s)	2830
Kzo9I	GATC	7 cut(s)	800, 1060, 1549, 2194, 2201, 2980, 3216
LguI	GCTCTTC	1 cut(s)	1898
LmnI	GCTCC	3 cut(s)	1777, 2346, 3406
Lsp1109I	GCAGC	6 cut(s)	406, 554, 892, 910, 1594, 3357
LweI	GCATC	4 cut(s)	604, 892, 2314, 2566
MaeI	CTAG	7 cut(s)	24, 231, 647, 894, 1700, 1982, 3198
MaeII	ACGT	3 cut(s)	225, 524, 2782
MaeIII	GTNAC	6 cut(s)	226, 583, 713, 2676, 3123, 3283
MalI	GATC	7 cut(s)	802, 1062, 1551, 2196, 2203, 2982, 3218
MboI	GATC	7 cut(s)	800, 1060, 1549, 2194, 2201, 2980, 3216
MfeI	CAATTG	2 cut(s)	2313, 2604
MhlI	GDGCHC	3 cut(s)	1167, 3039, 3319
MlyI	GAGTC	8 cut(s)	836, 1185, 1952, 1966, 2153, 2897, 3131, 3164
MmeI	TCCRAC	1 cut(s)	114
MroXI	GAANNNNTTC	3 cut(s)	1349, 1361, 1461
MslI	CAYNNNNRTG	4 cut(s)	450, 963, 2439, 3089
MspA1I	CMGCKG	3 cut(s)	243, 686, 3014
MspI	CCGG	2 cut(s)	202, 774
MspR9I	CCNGG	4 cut(s)	1066, 2199, 2396, 3225
MunI	CAATTG	2 cut(s)	2313, 2604
Mva1269I	GAATGC	1 cut(s)	1090
MvaI	CCWGG	4 cut(s)	1066, 2199, 2396, 3225
NcoI	CCATGG	1 cut(s)	3176
NdeI	CATATG	1 cut(s)	2923
NdeII	GATC	7 cut(s)	800, 1060, 1549, 2194, 2201, 2980, 3216
NlaIV	GGNNCC	4 cut(s)	490, 1516, 2828, 3306
NmeAIII	GCCGAG	2 cut(s)	367, 418
NmuCI	GTSAC	4 cut(s)	226, 713, 2676, 3123
NsbI	TGCGCA	1 cut(s)	2673
NspI	RCATGY	5 cut(s)	1486, 1652, 2438, 2495, 2509
PagI	TCATGA	1 cut(s)	481
PasI	CCCWGGG	1 cut(s)	1065
PciI	ACATGT	1 cut(s)	1482
PciSI	GCTCTTC	1 cut(s)	1898
PctI	GAATGC	1 cut(s)	1090
PdmI	GAANNNNTTC	3 cut(s)	1349, 1361, 1461
PfeI	GAWTC	7 cut(s)	193, 1243, 1253, 1708, 2627, 2663, 3043
PfoI	TCCNGGA	1 cut(s)	2197
PkrI	GCNGC	7 cut(s)	178, 421, 544, 907, 925, 1609, 3347
PleI	GAGTC	8 cut(s)	836, 1184, 1951, 1965, 2152, 2897, 3130, 3163
PpsI	GAGTC	8 cut(s)	836, 1184, 1951, 1965, 2152, 2897, 3130, 3163
PscI	ACATGT	1 cut(s)	1482
PshBI	ATTAAT	1 cut(s)	2358
Psp124BI	GAGCTC	1 cut(s)	1167
Psp1406I	AACGTT	2 cut(s)	524, 2782
Psp6I	CCWGG	4 cut(s)	1064, 2197, 2394, 3223
PspEI	GGTNACC	1 cut(s)	583
PspFI	CCCAGC	3 cut(s)	243, 2487, 2609
PspGI	CCWGG	4 cut(s)	1064, 2197, 2394, 3223
PspN4I	GGNNCC	4 cut(s)	490, 1516, 2828, 3306
PspPI	GGNCC	8 cut(s)	199, 489, 1068, 1596, 1835, 2024, 3141, 3304
PstNI	CAGNNNCTG	2 cut(s)	1946, 2344
PvuII	CAGCTG	2 cut(s)	243, 686
RsaI	GTAC	6 cut(s)	2073, 2310, 2372, 2504, 2828, 2858
RsaNI	GTAC	6 cut(s)	2072, 2309, 2371, 2503, 2827, 2857
RseI	CAYNNNNRTG	4 cut(s)	450, 963, 2439, 3089
SacI	GAGCTC	1 cut(s)	1167
SapI	GCTCTTC	1 cut(s)	1898
SatI	GCNGC	7 cut(s)	177, 420, 543, 906, 924, 1608, 3346
Sau3AI	GATC	7 cut(s)	800, 1060, 1549, 2194, 2201, 2980, 3216
Sau96I	GGNCC	8 cut(s)	199, 489, 1068, 1596, 1835, 2024, 3141, 3304
ScaI	AGTACT	1 cut(s)	2372
SchI	GAGTC	8 cut(s)	836, 1185, 1952, 1966, 2153, 2897, 3131, 3164
ScrFI	CCNGG	4 cut(s)	1066, 2199, 2396, 3225
SduI	GDGCHC	3 cut(s)	1167, 3039, 3319
SfaNI	GCATC	4 cut(s)	604, 892, 2314, 2566
SfcI	CTRYAG	3 cut(s)	302, 706, 3117
SinI	GGWCC	4 cut(s)	489, 1835, 3141, 3304
SmiMI	CAYNNNNRTG	4 cut(s)	450, 963, 2439, 3089
SmlI	CTYRAG	3 cut(s)	185, 2552, 3159
SmoI	CTYRAG	3 cut(s)	185, 2552, 3159
SpeI	ACTAGT	1 cut(s)	23
SsiI	CCGC	9 cut(s)	133, 165, 177, 265, 380, 473, 2758, 2851, 3014
SspI	AATATT	1 cut(s)	3137
SspMI	CTAG	7 cut(s)	24, 231, 647, 894, 1700, 1982, 3198
SstI	GAGCTC	1 cut(s)	1167
StyD4I	CCNGG	4 cut(s)	1064, 2197, 2394, 3223
StyI	CCWWGG	4 cut(s)	646, 1699, 2860, 3176
TaaI	ACNGT	6 cut(s)	589, 782, 1745, 2078, 3118, 3321
TaiI	ACGT	3 cut(s)	228, 527, 2785
TaqI	TCGA	3 cut(s)	253, 1179, 2209
TaqII	GACCGA	1 cut(s)	1823
TatI	WGTACW	2 cut(s)	2370, 2502
TauI	GCSGC	1 cut(s)	179
TfiI	GAWTC	7 cut(s)	193, 1243, 1253, 1708, 2627, 2663, 3043
TseFI	GTSAC	4 cut(s)	226, 713, 2676, 3123
TseI	GCWGC	6 cut(s)	419, 542, 905, 923, 1607, 3345
Tsp45I	GTSAC	4 cut(s)	226, 713, 2676, 3123
TspGWI	ACGGA	1 cut(s)	442
VneI	GTGCAC	1 cut(s)	3315
VpaK11BI	GGWCC	4 cut(s)	489, 1835, 3141, 3304
VspI	ATTAAT	1 cut(s)	2358
XapI	RAATTY	3 cut(s)	954, 1393, 3165
XceI	RCATGY	5 cut(s)	1486, 1652, 2438, 2495, 2509
XmaJI	CCTAGG	2 cut(s)	646, 1699
XmnI	GAANNNNTTC	3 cut(s)	1349, 1361, 1461
XspI	CTAG	7 cut(s)	24, 231, 647, 894, 1700, 1982, 3198
ZrmI	AGTACT	1 cut(s)	2372

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.