Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0013983

Physical Location & Seq

Genomic Coordinates Forward (+)

57059408 .. 57076081

Gene Structure 16674 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G032810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5421 bp

ATGGAGCCACCGGAGTGCCCAGTGTGTCTGCAGAACTACGACGGCGCGTCGACAATTCCGCGCGTGCTTGGTTGCGGCCACTCGGCTTGCGAGGTCTGCCTGGCGAAGCTGCCCCACCGCTACCCGGAGACGATTCGCTGCCCGGCGTGTACTCAGCTCGTCAAGTACCCTCAGAACGGCGCCGCCGCGCTGCCCAAGAACATCGACCTTCTCTCGCTCTCTCTCTCCCTCTCTCCGCCGCAAAACCCTAACCCTAATTCCGACGCTCCCCGGAAGCGACGTCAGCAATCCGCCGATGATTTAGAAGTGTTTAATTTCCTGCCGCGGCTATGGTCCGACGAGTTTTATAGTGCTTGGAAGGACTGGGTTGTTGGGTACGACGCCGTTTTGGTCGAAACTGAAGAAAGGGCTCGGCTTTGTGATTCTGGTGCTGCTGTGGGGTTAGTTCAAGTTGGGTTTTTGGAGAGATTGGGTGAGTCTAAATTTGAGTTTAGCTACAATGTGAGGGTTATGAAGTGTTTGAGTGGAATGAGAGAGGAGGAGAGGAGAGAATTGGGTTTGTTGCTGAGAGGTTCTGGGAGGCAGTGTAGGAGGATAGGGAAGGTTTATGGGTTTTGGGGAAATGTGGAAGATGGGGTTTTGTATTTAGTGTGTGAGAGCCAGAGTGGGAGGTTTGAGGAGAAGTTGAGGGGTTTGAGAAATGGGGATGGGTTTGGGAGAGATGGGTTGGGTGGTTTTGGTGTGATTGGTATGGAGGTGTGTGAGGCGGTGATGGGGTTGCATTCGGAGGGGTTTGTTGGTGGTGGTTTTGGTGTTTCGTGTTTTGGTTTCGATGATTTTGGTCATGTTTTTGTGGATTTGAATGAGGTGTTGGTGATGGGAAGGAAGGTTTGGACAAGTGTTGGGGATAGTGTTGGTGGTAGAAAGGGAATTGGTGCTGAAGAAATGGGAGTGATGTTTGGCAGTTTATTGAAAGATGGGGCTTTTGTTAGCATGGAGGTGTTGTTTGAGGTGTTGAAGAAAGAGGGCATTGCAGTGGAATGTGAGAGCTCGAAATATATGGTTGGGTATGGCTCGGATGTTTGGTCATTAGCTTGTGTTTTGCTCAGTCTGCTTCTTGGGAAAGAGTTCAGTGAAGAGATTGGAAATATGAATCATATTTGCGATCATTCAACGTATGCAAGTTGGATTGAAAGAGTTGGTGATTTGCTGGACAGTAGATTGGGTTCTGAATATGCATCACTGAGGGTGACTCTCTGTAAATGTTTGAATTATGATCCAGCAAGTCGTCCACTAGTGATTGATGTTATGATTTGCATTAGGGAACTGATTATTAAACCTCAATATGATATCATGGCCGGTTTGGAGAGACCGGTTAAGGAGAACAGCACAAGTTGTTGCTTGATTCTGGGGGAGCTCTGTCAGATGCCCAAGAAAATGTCCGAAACACAGAAAGAACATGAGTTGCAGGGAAAAGAAGTTGGTGGAGGAGCAGACTTTGATCAGGTTGATGAGGGGAGGTCTGAGAATGGTTTTGTTGATGGACTCGCAGAGGGAAAGGTCAAATCTAAAGTTCTGCAGGGCCATCGTGACTCTATTACAGCATTAGCTGTTGGAGGAGAATTTCTATTCAGCTCCTCATTTGACAAAACCATCCATGTGTGGTCTTTGCAGGACTTCTGTCATGTACATACATTTAAAGGCCATGAGCATACCATTAAGGCTCTAATTTATGTAGATGAAGAGAAACCCTTGTGCATAAGTGGTGACAGTGGAGGTGGCATATTTGTCTGGAGCACAAGTTCCCCTCTAGGGCAAGAACCATTGAAGATACTGTGTGAGCAGAAAGATTGGCGCTTTAGTGGTATTCATGCCTTGGCCTTTAGAGATGGATATATTTATACCGGCAGTGGAGACAGAACAGTAAAAGCATGGTCTATGCAGGACGGCACCATATCATGCACTATGTCTGGTCATAAATCAGTAGTTTCAACACTTACAGTTTGTGATAGTGTTCTGTATAGCGGCAGTTGGGATGGAACCATACGATTATGGAGTCTCAGCGATCACAGTCCTTTGGCAGTACTAGGGGAAGACACATCTGGAACTGTGGCTTCTGTCTTATCTCTTGCTGCTGACAGACATGTGCTCATTGCAACCCATGAAAATGGATGTCTAAAGGTCTGGAAAAATGATGTGTTTATGAAATCCATTAAAATGCACAATGGTGCAGTTTTTACTACCGGCCTGGATGGAAAATGGCTTTTCACAGGCGGGTTGGATAAAACTGTCAATGTCCAGGAATGGTCTGGTGATGAGTTTCAAACAGATTTCAGACCTATTGGATCCATCCCCTGTGATTCTGTCATTACAACTTTGTTGTGCTGGCAAGGAAAGCTTTTTGTTGGATATGCTAATAGGAATATCATGGAGAATCTGGGCCGGATTGGAAAGACTTGGAGCGACGTGACTAGCTTGAACAGCTGGGTTGTTCGAGACTACTACCGCCTTGTCAATTCTGTCAACTCTTTTGAGCCACAGCTACAGAGGCTCACTGATGACCAGCTTACTGCTAAAACTGCCGAGTTTCGGCGAAGATTGGGACAAGGAGAGACACTTGCGGATATTCAAGCCGAGGCTTTTGCTGTTGTTCGTGAAGCTGCGAAACGGAAGCTTGGTATGCGTCATTTTGATGTTCAGATTATTGGTGGGGCGGTGCTTCATGATGGGTCCATTGCAGAAATGAAGACCGGAGAAGGGAAAACGTTGGTTTCCACATTAGCAGCATATCTTAATGCCCTGACTGGCGAAGGTGTTCATGTGGTAACCGTAAATGATTACCTTGCTCAAAGGGATGCTGAGTGGATGGGTCGTGTTCATCGCTTCCTAGGTCTTTCAGTGGGTCTTATTCAGAGAGGGATGACAGCTGACAAGAGGAGGTCAAACTATAGATGTGACATAACATACACCAATAATTCAGAACTTGGGTTTGACTATCTACGAGACAATCTTGCCGGAAACAGTGGAGAACTTGTTATGAGATCGCCAAAGCCATTTCATTTTGCAATAGTTGATGAAGTTGACTCAGTTCTTATTGATGAAGGAAGAAATCCATTGCTAATTAGTGGCGAGGCTAGTAAAGATGCTGCTCGCTATCCTGTTGCTGCTAAAGTGGCTGAGTTGCTTGTGCGAGGAATTCATTACAATGTAGAACTTAAAGATTACTCAGTTGAGTTGACTGAAGAAGGAATAGCTCTTGCTGAGATGGCTCTTGAAACAAATGACTTATGGGATGAAAATGATCCCTGGGCCAGATTTGTGATGAATGCGTTGAAAGCTAAAGAGTTCTATCGGAAGGATGTGCAATATATTGTCAGAAATGGGAAGGCTCTCATAATCAATGAGCTCACTGGCAGAGTCGAAGATAAGAGAAGGTGGTCTGAGGGTATTCATCAAGCTGTGGAGGGAAAAGAAGGTTTGAAGATTCAGGCTGATTCGGTTGTTATCGCACAAATCACATATCAATCTCTGTTCAAGCTCTACCCAAAGCTATCAGGGATGACTGGCACTGCAAAAACTGAAGAGAAAGAGTTCTTGAAAATGTTCCAAACACCAGTTATTGAAGTACCAACAAATTTGCCAAACATTCGTAATGATTTACCTATTCAAGCTTTTGCGACTGCTCAGGGGAAATGGGAATATGTTCGTCAAGAAGTGGAATACATGTTCAGGCAGGGGCGTCCTGTTTTGGTTGGCACCACCAGTGTTGAGTATTCTGAACACCTGTCTGATCTGTTGAAGGAACATAACATCCCACACAATGTTCTAAATGCACGGCCCAAGTATGCTGCAAGGGAGGCTGAAATTGTTGCCCAAGCTGGACGAAAACATGCCATTACTCTTTCTACTAATATGGCTGGCAGAGGCACTGACATAATCCTAGGGGGGAATCCTAAAATGCTTGCAAAAGAAATCATAGAAGACAGTTTGATTTCATTCCTGACACGAGAAGCTCCTAACATTGACATAGATGGAGAGGCAATTTCACAAAAGGTATTGTCAAAGATTAAGGTCGGTCCATCATCATTAGCTTTGCTGGCTAAGACAGCGTTAATGGCTAAATATGTTGGCAAAAATGAAGGGAAGAGCTGGACATATAAGGAGGCAAAATCCATGATATCAGAGTCTGTGGAAATGAGTCAATCAAGAGATTTGAAAGAGCTAGAGAGGCTTGTTGATGAGCAGTCTGAGATATACCCTCTTGGCCCTACAATAGCACTTGCTTATCTGTCAGTTTTGAAGGATTGCGAGGTACACTGTTTCAAAGAAGGATCTGAAGTGAAAAGACTTGGGGGACTTCATGTGATTGGAACATCTTTGCACGAGTCTCGTAGAATAGATAACCAGCTTCGTGGCAGAGCAGGAAGGCAAGGTGATCCAGGATCAACTCGATTTATGGTGAGCTTGCAGGATGAGATGTTTCAAAAGTTTAATTTTGATACTGAGTGGGCTGTGAGACTTATTTCAAAAATTACTGATGATGAGGATGTACCAATTGAAGGTGATGCCATTGTGAGACAGCTCCTGTCCCTGCAAATTAATGCTGAAAAGTACTTTTTTGGCATAAGAAAGAGCCTGGTTGAGTTTGATGAAGTATTAGAGGTGCAGAGAAAGCATGTTTATGAACTTCGGCAGTCAATTTTAACTGGTGATAATGAAAGTTGTTCCCAGCATGTATTCCAGTACATGCAAGCAGTGGTAGATGAAATTGTCTTTAAAAATGTTGACACCCTCAAGCATCCAAGAAAATGGAGTTTAAATAAGCTTTTGAAGGAGTTTATGGCAATTGCTGGGAAACTATTGGATGATTCATTTGCAGGAATTACTGAGGACGCTTTGTTAGAATCTCTTGCGCAGTCACATGAACTGAACTTTAAAGATATTGATGACATTCACCTTCCAAACTTGCCAATACCTCCAAATGCCTTCAGAGGTATCCGCAAGAAGAACTCTTCATTGAAACGTTGGCTTGCTATGTGCTCGGATGATTTGACCAAGAATGGAAGGTACCATGCTACTACTAACCTCCTCCGCAAGTACCTTGGAGACTATTTGATTGCTTCATATTTGGATGTTGTTCAAGACTCTGGTTATGATGATACATATGTGAAGGAAGTTGAAAGAGCAGTTATTGTGAAAACCCTTGATTGTTTCTGGAGGGATCACCTTGTAAACATGAACAGGCTAAGTTCAGCGGTGAATGTAAGGAGTTTTGGGCACAGGAATCCTCTTGAAGAATACAAGATTGATGGTTGTCGTTTTTTCATATCCATGCTTAGTGCAACACGAAGGTTGACTGTAGAGTCACTTTTGCAATATTGGTCCTCCCCAATGGAGTCTCAAGAAATTTTTGTATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0006996 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0009657 GO:0009658 GO:0009987 GO:0010109 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019222 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0031323 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW WD40 zf-RING_UBOX

Protein Analysis

1806

Amino Acids

201.71

Weight (kDa)

5.66

Isoelectric Point (pI)

38.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_UBOX	PF13445	6 - 48	1.6e-06	RING-type zinc-finger
WDR55	PF24796	517 - 730	6.2e-13	WDR55
WD40_Gbeta	PF25391	523 - 685	9.9e-08	G protein beta WD-40 repeat protein
Beta-prop_SCAP	PF24017	524 - 599	6.4e-06	SCAP Beta-propeller
Beta-prop_WDR3_1st	PF25173	525 - 598	4.7e-08	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	526 - 744	3.9e-23	WDR5 beta-propeller domain
WD40_Prp19	PF24814	621 - 732	2.4e-08	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	631 - 745	3.1e-06	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	632 - 766	3.9e-13	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	632 - 696	1.9e-06	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	632 - 693	2.6e-07	WDHD1 first WD40 domain
SecA_DEAD	PF07517	829 - 1198	4.8e-116	SecA DEAD-like domain
SecA_PP_bind	PF01043	1049 - 1155	1.1e-28	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	1214 - 1501	1.1e-65	SecA P-loop domain
SecA_SW	PF07516	1518 - 1792	8.8e-42	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21650 AT1G21650 AT1G21650
fragaria_vesca	FvH4_2g27750
malus_domestica	MD00G1100600.v1.1
prunus_persica	Prupe.1G490400_v2.0.a1
pyrus_communis	pycom15g30570
rosa_chinensis	RchiOBHm_Chr6g0296621
rosa_laevigata	RLG00000011676
rosa_multiflora	Rmu_sc0001741.1_g000019
rosa_roxburghii	Rroxscaffold_7G00171330 Rroxscaffold_7G00178770
rosa_rugosa	Rorug06G0263700
rosa_samantha	Rh6AG375100 Rh6CG388700
rosa_wichuraiana	Rw6G032720 Rw6G032810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	5362
AatII	GACGTC	1 cut(s)	283
Acc16I	TGCGCA	1 cut(s)	4914
Acc65I	GGTACC	1 cut(s)	5067
AccB1I	GGYRCC	4 cut(s)	179, 1949, 3755, 5067
AccI	GTMKAC	1 cut(s)	50
AccII	CGCG	5 cut(s)	47, 61, 63, 188, 325
AclI	AACGTT	2 cut(s)	2765, 5023
AclWI	GGATC	8 cut(s)	1271, 2340, 2353, 3296, 4339, 4430, 4450, 5229
AcoI	YGGCCR	2 cut(s)	76, 1356
AcsI	RAATTY	5 cut(s)	482, 1622, 3195, 3634, 5406
AcuI	CTGAAG	6 cut(s)	420, 960, 3261, 3600, 4356, 4972
AcyI	GRCGYC	4 cut(s)	180, 280, 381, 3739
AfiI	CCNNNNNNNGG	6 cut(s)	124, 176, 1812, 2589, 2757, 4559
AflIII	ACRYGT	2 cut(s)	2143, 3723
AgeI	ACCGGT	1 cut(s)	1372
AhdI	GACNNNNNGTC	1 cut(s)	46
AhlI	ACTAGT	1 cut(s)	1294
AjiI	CACGTC	1 cut(s)	2467
AjnI	CCWGG	6 cut(s)	99, 2247, 2298, 3305, 4438, 4635
AjuI	GAANNNNNNNTTGG	4 cut(s)	854, 874, 886, 906
AleI	CACNNNNGTG	2 cut(s)	13, 2226
AloI	GAACNNNNNNTCC	6 cut(s)	1786, 1818, 3710, 3742, 3795, 3827
Alw21I	GWGCWC	6 cut(s)	1052, 1419, 1799, 2151, 3408, 5042
AlwI	GGATC	8 cut(s)	1271, 2340, 2353, 3296, 4339, 4430, 4450, 5229
AlwNI	CAGNNNCTG	2 cut(s)	4187, 4585
ApoI	RAATTY	5 cut(s)	482, 1622, 3195, 3634, 5406
AseI	ATTAAT	1 cut(s)	4599
AsiGI	ACCGGT	1 cut(s)	1372
Asp700I	GAANNNNTTC	5 cut(s)	1127, 2814, 3590, 3602, 3702
Asp718I	GGTACC	1 cut(s)	5067
AspA2I	CCTAGG	2 cut(s)	2887, 3940
AspLEI	GCGC	6 cut(s)	47, 63, 182, 190, 1857, 4915
AspS9I	GGNCC	9 cut(s)	333, 1582, 2440, 2730, 3309, 3837, 4076, 4265, 5382
AsuC2I	CCSGG	3 cut(s)	125, 143, 271
AvaII	GGWCC	4 cut(s)	333, 2730, 4076, 5382
AvrII	CCTAGG	2 cut(s)	2887, 3940
BaeGI	GKGCMC	2 cut(s)	20, 5280
BaeI	ACNNNNGTAYC	2 cut(s)	5151, 5184
BamHI	GGATCC	1 cut(s)	2345
BanI	GGYRCC	4 cut(s)	179, 1949, 3755, 5067
BanII	GRGCYC	4 cut(s)	412, 1052, 1419, 3408
BauI	CACGAG	2 cut(s)	4005, 4382
BbsI	GAAGAC	3 cut(s)	2100, 2753, 3987
Bbv12I	GWGCWC	6 cut(s)	1052, 1419, 1799, 2151, 3408, 5042
BceAI	ACGGC	5 cut(s)	58, 193, 368, 1963, 3851
BcgI	CGANNNNNNTGC	6 cut(s)	1568, 1602, 2624, 2658, 4370, 4404
BciT130I	CCWGG	6 cut(s)	101, 2249, 2300, 3307, 4440, 4637
BciVI	GTATCC	1 cut(s)	5006
BclI	TGATCA	1 cut(s)	1501
BcnI	CCSGG	3 cut(s)	125, 143, 271
BcuI	ACTAGT	1 cut(s)	1294
BfaI	CTAG	8 cut(s)	1295, 1811, 2087, 2472, 2888, 3135, 3941, 4223
BfmI	CTRYAG	5 cut(s)	29, 1577, 2543, 2947, 5358
BfoI	RGCGCY	2 cut(s)	183, 1858
BfuI	GTATCC	1 cut(s)	5006
BlnI	CCTAGG	2 cut(s)	2887, 3940
BmcAI	AGTACT	2 cut(s)	2085, 4613
Bme1390I	CCNGG	9 cut(s)	101, 125, 143, 271, 2249, 2300, 3307, 4440, 4637
Bme18I	GGWCC	4 cut(s)	333, 2730, 4076, 5382
BmeRI	GACNNNNNGTC	1 cut(s)	46
BmgBI	CACGTC	1 cut(s)	2467
BmgT120I	GGNCC	9 cut(s)	333, 1582, 2440, 2730, 3309, 3837, 4076, 4265, 5382
BmiI	GGNNCC	8 cut(s)	6, 181, 1951, 2041, 2347, 2731, 3757, 5069
BmrFI	CCNGG	9 cut(s)	101, 125, 143, 271, 2249, 2300, 3307, 4440, 4637
BmrI	ACTGGG	2 cut(s)	14, 373
BmsI	GCATC	6 cut(s)	1247, 1416, 2845, 3133, 4555, 4807
BmuI	ACTGGG	2 cut(s)	14, 373
BoxI	GACNNNNGTC	1 cut(s)	1680
BpiI	GAAGAC	3 cut(s)	2100, 2753, 3987
BplI	GAGNNNNNCTC	2 cut(s)	1237, 1269
BpmI	CTGGAG	2 cut(s)	1813, 5236
Bpu10I	CCTNAGC	1 cut(s)	3684
BpuEI	CTTGAG	2 cut(s)	4778, 5385
BpuMI	CCSGG	3 cut(s)	125, 143, 271
BsaHI	GRCGYC	4 cut(s)	180, 280, 381, 3739
BsaI	GGTCTC	1 cut(s)	1363
BsaJI	CCNNGG	9 cut(s)	269, 323, 1875, 2634, 2887, 3305, 3306, 3940, 5101
BsaWI	WCCGGW	3 cut(s)	10, 1372, 2750
BsaXI	ACNNNNNCTCC	8 cut(s)	571, 601, 989, 1019, 1786, 1816, 3018, 3048
Bsc4I	CCNNNNNNNGG	6 cut(s)	124, 176, 1812, 2589, 2757, 4559
Bse118I	RCCGGY	4 cut(s)	1358, 1372, 1904, 2243
Bse1I	ACTGG	9 cut(s)	20, 368, 2809, 3415, 3568, 3614, 3762, 4711, 4741
Bse3DI	GCAATG	4 cut(s)	1029, 2151, 2733, 3113
BseBI	CCWGG	6 cut(s)	101, 2249, 2300, 3307, 4440, 4637
BseDI	CCNNGG	9 cut(s)	269, 323, 1875, 2634, 2887, 3305, 3306, 3940, 5101
BseLI	CCNNNNNNNGG	6 cut(s)	124, 176, 1812, 2589, 2757, 4559
BseMI	GCAATG	4 cut(s)	1029, 2151, 2733, 3113
BseNI	ACTGG	9 cut(s)	20, 368, 2809, 3415, 3568, 3614, 3762, 4711, 4741
BseRI	GAGGAG	9 cut(s)	551, 554, 559, 692, 1503, 1627, 1632, 2950, 5078
BseSI	GKGCMC	2 cut(s)	20, 5280
BseYI	CCCAGC	3 cut(s)	2484, 4728, 4850
BsgI	GTGCAG	2 cut(s)	2250, 4685
Bsh1236I	CGCG	5 cut(s)	47, 61, 63, 188, 325
BshNI	GGYRCC	4 cut(s)	179, 1949, 3755, 5067
BshTI	ACCGGT	1 cut(s)	1372
BsiHKAI	GWGCWC	6 cut(s)	1052, 1419, 1799, 2151, 3408, 5042
BslFI	GGGAC	3 cut(s)	2616, 4368, 4573
BslI	CCNNNNNNNGG	6 cut(s)	124, 176, 1812, 2589, 2757, 4559
BsmBI	CGTCTC	1 cut(s)	122
BsmFI	GGGAC	3 cut(s)	2616, 4368, 4573
BsmI	GAATGC	2 cut(s)	781, 3331
Bso31I	GGTCTC	1 cut(s)	1363
Bsp1286I	GDGCHC	9 cut(s)	20, 412, 1052, 1419, 1799, 2151, 3408, 5042, 5280
Bsp1407I	TGTACA	1 cut(s)	1687
BspFNI	CGCG	5 cut(s)	47, 61, 63, 188, 325
BspHI	TCATGA	2 cut(s)	2722, 5417
BspLI	GGNNCC	8 cut(s)	6, 181, 1951, 2041, 2347, 2731, 3757, 5069
BspMAI	CTGCAG	2 cut(s)	33, 1581
BspPI	GGATC	8 cut(s)	1271, 2340, 2353, 3296, 4339, 4430, 4450, 5229
BspQI	GCTCTTC	1 cut(s)	4139
BspT107I	GGYRCC	4 cut(s)	179, 1949, 3755, 5067
BspTNI	GGTCTC	1 cut(s)	1363
BsrDI	GCAATG	4 cut(s)	1029, 2151, 2733, 3113
BsrFI	RCCGGY	4 cut(s)	1358, 1372, 1904, 2243
BsrGI	TGTACA	1 cut(s)	1687
BsrI	ACTGG	9 cut(s)	20, 368, 2809, 3415, 3568, 3614, 3762, 4711, 4741
BssAI	RCCGGY	4 cut(s)	1358, 1372, 1904, 2243
BssECI	CCNNGG	9 cut(s)	269, 323, 1875, 2634, 2887, 3305, 3306, 3940, 5101
BssNI	GRCGYC	4 cut(s)	180, 280, 381, 3739
BssSI	CACGAG	2 cut(s)	4005, 4382
BssT1I	CCWWGG	4 cut(s)	1875, 2887, 3940, 5101
Bst2BI	CACGAG	2 cut(s)	4005, 4382
Bst2UI	CCWGG	6 cut(s)	101, 2249, 2300, 3307, 4440, 4637
Bst6I	CTCTTC	5 cut(s)	1131, 1737, 3576, 4139, 5017
BstACI	GRCGYC	4 cut(s)	180, 280, 381, 3739
BstAUI	TGTACA	1 cut(s)	1687
BstDSI	CCRYGG	1 cut(s)	323
BstEII	GGTNACC	1 cut(s)	2824
BstFNI	CGCG	5 cut(s)	47, 61, 63, 188, 325
BstH2I	RGCGCY	2 cut(s)	183, 1858
BstHHI	GCGC	6 cut(s)	47, 63, 182, 190, 1857, 4915
BstNI	CCWGG	6 cut(s)	101, 2249, 2300, 3307, 4440, 4637
BstNSI	RCATGY	6 cut(s)	2147, 3727, 3893, 4679, 4736, 4750
BstPAI	GACNNNNGTC	1 cut(s)	1680
BstPI	GGTNACC	1 cut(s)	2824
BstSCI	CCNGG	9 cut(s)	99, 123, 141, 269, 2247, 2298, 3305, 4438, 4635
BstSFI	CTRYAG	5 cut(s)	29, 1577, 2543, 2947, 5358
BstSLI	GKGCMC	2 cut(s)	20, 5280
BstUI	CGCG	5 cut(s)	47, 61, 63, 188, 325
BstV2I	GAAGAC	3 cut(s)	2100, 2753, 3987
BstX2I	RGATCY	2 cut(s)	2345, 4331
BstXI	CCANNNNNNTGG	3 cut(s)	2168, 4412, 4809
BstYI	RGATCY	2 cut(s)	2345, 4331
BsuI	GTATCC	1 cut(s)	5006
BtgI	CCRYGG	1 cut(s)	323
BtgZI	GCGATG	1 cut(s)	2864
BtrI	CACGTC	1 cut(s)	2467
BtsI	GCAGTG	5 cut(s)	590, 1041, 1915, 3567, 4761
CaiI	CAGNNNCTG	2 cut(s)	4187, 4585
CciI	TCATGA	2 cut(s)	2722, 5417
CfoI	GCGC	6 cut(s)	47, 63, 182, 190, 1857, 4915
Cfr10I	RCCGGY	4 cut(s)	1358, 1372, 1904, 2243
Cfr13I	GGNCC	9 cut(s)	333, 1582, 2440, 2730, 3309, 3837, 4076, 4265, 5382
Cfr42I	CCGCGG	1 cut(s)	326
CseI	GACGC	6 cut(s)	36, 272, 389, 2672, 3728, 4901
CspAI	ACCGGT	1 cut(s)	1372
CspCI	CAANNNNNGTGG	4 cut(s)	68, 103, 3748, 3783
DinI	GGCGCC	1 cut(s)	181
DraI	TTTAAA	4 cut(s)	1699, 4777, 4818, 4936
DrdI	GACNNNNNNGTC	1 cut(s)	5362
DriI	GACNNNNNGTC	1 cut(s)	46
DseDI	GACNNNNNNGTC	1 cut(s)	5362
EaeI	YGGCCR	2 cut(s)	76, 1356
Eam1104I	CTCTTC	5 cut(s)	1131, 1737, 3576, 4139, 5017
Eam1105I	GACNNNNNGTC	1 cut(s)	46
EarI	CTCTTC	5 cut(s)	1131, 1737, 3576, 4139, 5017
EciI	GGCGGA	2 cut(s)	225, 280
Ecl136II	GAGCTC	3 cut(s)	1050, 1417, 3406
Eco130I	CCWWGG	4 cut(s)	1875, 2887, 3940, 5101
Eco24I	GRGCYC	4 cut(s)	412, 1052, 1419, 3408
Eco31I	GGTCTC	1 cut(s)	1363
Eco32I	GATATC	2 cut(s)	1351, 4179
Eco47I	GGWCC	4 cut(s)	333, 2730, 4076, 5382
Eco53kI	GAGCTC	3 cut(s)	1050, 1417, 3406
Eco57I	CTGAAG	6 cut(s)	420, 960, 3261, 3600, 4356, 4972
Eco91I	GGTNACC	1 cut(s)	2824
EcoICRI	GAGCTC	3 cut(s)	1050, 1417, 3406
EcoO65I	GGTNACC	1 cut(s)	2824
EcoRI	GAATTC	1 cut(s)	3195
EcoRII	CCWGG	6 cut(s)	99, 2247, 2298, 3305, 4438, 4635
EcoRV	GATATC	2 cut(s)	1351, 4179
EcoT14I	CCWWGG	4 cut(s)	1875, 2887, 3940, 5101
EcoT22I	ATGCAT	1 cut(s)	1240
EcoT38I	GRGCYC	4 cut(s)	412, 1052, 1419, 3408
EgeI	GGCGCC	1 cut(s)	181
EheI	GGCGCC	1 cut(s)	181
ErhI	CCWWGG	4 cut(s)	1875, 2887, 3940, 5101
Esp3I	CGTCTC	1 cut(s)	122
FalI	AAGNNNNNCTT	2 cut(s)	2382, 2414
FaqI	GGGAC	3 cut(s)	2616, 4368, 4573
FauI	CCCGC	1 cut(s)	2267
FauNDI	CATATG	1 cut(s)	5164
FbaI	TGATCA	1 cut(s)	1501
FblI	GTMKAC	1 cut(s)	50
FriOI	GRGCYC	4 cut(s)	412, 1052, 1419, 3408
FspBI	CTAG	8 cut(s)	1295, 1811, 2087, 2472, 2888, 3135, 3941, 4223
FspI	TGCGCA	1 cut(s)	4914
GlaI	GCGC	6 cut(s)	46, 62, 181, 189, 1856, 4914
GsaI	CCCAGC	3 cut(s)	2488, 4732, 4854
GsuI	CTGGAG	2 cut(s)	1813, 5236
HaeII	RGCGCY	2 cut(s)	183, 1858
HgaI	GACGC	6 cut(s)	36, 272, 389, 2672, 3728, 4901
HhaI	GCGC	6 cut(s)	47, 63, 182, 190, 1857, 4915
Hin1I	GRCGYC	4 cut(s)	180, 280, 381, 3739
Hin6I	GCGC	6 cut(s)	45, 61, 180, 188, 1855, 4913
HinP1I	GCGC	6 cut(s)	45, 61, 180, 188, 1855, 4913
HincII	GTYRAC	6 cut(s)	51, 2524, 3082, 3237, 4786, 5355
HindII	GTYRAC	6 cut(s)	51, 2524, 3082, 3237, 4786, 5355
HindIII	AAGCTT	4 cut(s)	2396, 2672, 3669, 4823
Hpy99I	CGWCG	7 cut(s)	44, 52, 266, 282, 341, 383, 2468
HpyCH4IV	ACGT	5 cut(s)	280, 1175, 2466, 2765, 5023
HpySE526I	ACGT	5 cut(s)	280, 1175, 2466, 2765, 5023
Hsp92I	GRCGYC	4 cut(s)	180, 280, 381, 3739
HspAI	GCGC	6 cut(s)	45, 61, 180, 188, 1855, 4913
KasI	GGCGCC	1 cut(s)	179
KpnI	GGTACC	1 cut(s)	5071
Ksp22I	TGATCA	1 cut(s)	1501
KspI	CCGCGG	1 cut(s)	326
LguI	GCTCTTC	1 cut(s)	4139
LmnI	GCTCC	9 cut(s)	4, 271, 1414, 1490, 1640, 1794, 2460, 4018, 4587
LweI	GCATC	6 cut(s)	1247, 1416, 2845, 3133, 4555, 4807
MaeI	CTAG	8 cut(s)	1295, 1811, 2087, 2472, 2888, 3135, 3941, 4223
MaeII	ACGT	5 cut(s)	280, 1175, 2466, 2765, 5023
MaeIII	GTNAC	8 cut(s)	1249, 1589, 1766, 2467, 2824, 2954, 4917, 5364
MfeI	CAATTG	2 cut(s)	4554, 4845
MflI	RGATCY	2 cut(s)	2345, 4331
MhlI	GDGCHC	9 cut(s)	20, 412, 1052, 1419, 1799, 2151, 3408, 5042, 5280
Mly113I	GGCGCC	1 cut(s)	180
MmeI	TCCRAC	6 cut(s)	285, 360, 1166, 1594, 2259, 2386
Mph1103I	ATGCAT	1 cut(s)	1240
MroXI	GAANNNNTTC	5 cut(s)	1127, 2814, 3590, 3602, 3702
MspA1I	CMGCKG	4 cut(s)	325, 2484, 2927, 5255
MspR9I	CCNGG	9 cut(s)	101, 125, 143, 271, 2249, 2300, 3307, 4440, 4637
MunI	CAATTG	2 cut(s)	4554, 4845
Mva1269I	GAATGC	2 cut(s)	781, 3331
MvaI	CCWGG	6 cut(s)	101, 2249, 2300, 3307, 4440, 4637
MvnI	CGCG	5 cut(s)	47, 61, 63, 188, 325
NarI	GGCGCC	1 cut(s)	180
NciI	CCSGG	3 cut(s)	125, 143, 271
NdeI	CATATG	1 cut(s)	5164
NlaIV	GGNNCC	8 cut(s)	6, 181, 1951, 2041, 2347, 2731, 3757, 5069
NmeAIII	GCCGAG	4 cut(s)	62, 391, 2608, 2659
NmuCI	GTSAC	7 cut(s)	1249, 1589, 1766, 2467, 2954, 4917, 5364
NsbI	TGCGCA	1 cut(s)	4914
NsiI	ATGCAT	1 cut(s)	1240
NspI	RCATGY	6 cut(s)	2147, 3727, 3893, 4679, 4736, 4750
OliI	CACNNNNGTG	2 cut(s)	13, 2226
PagI	TCATGA	2 cut(s)	2722, 5417
PasI	CCCWGGG	1 cut(s)	3306
PciI	ACATGT	2 cut(s)	2143, 3723
PciSI	GCTCTTC	1 cut(s)	4139
PctI	GAATGC	2 cut(s)	781, 3331
PdmI	GAANNNNTTC	5 cut(s)	1127, 2814, 3590, 3602, 3702
PfoI	TCCNGGA	2 cut(s)	2298, 4438
PinAI	ACCGGT	1 cut(s)	1372
PluTI	GGCGCC	1 cut(s)	183
PscI	ACATGT	2 cut(s)	2143, 3723
PshAI	GACNNNNGTC	1 cut(s)	1680
PshBI	ATTAAT	1 cut(s)	4599
Psp124BI	GAGCTC	3 cut(s)	1052, 1419, 3408
Psp1406I	AACGTT	2 cut(s)	2765, 5023
Psp6I	CCWGG	6 cut(s)	99, 2247, 2298, 3305, 4438, 4635
PspEI	GGTNACC	1 cut(s)	2824
PspFI	CCCAGC	3 cut(s)	2484, 4728, 4850
PspGI	CCWGG	6 cut(s)	99, 2247, 2298, 3305, 4438, 4635
PspN4I	GGNNCC	8 cut(s)	6, 181, 1951, 2041, 2347, 2731, 3757, 5069
PspPI	GGNCC	9 cut(s)	333, 1582, 2440, 2730, 3309, 3837, 4076, 4265, 5382
PsrI	GAACNNNNNNTAC	2 cut(s)	1210, 1242
PstI	CTGCAG	2 cut(s)	33, 1581
PstNI	CAGNNNCTG	2 cut(s)	4187, 4585
PsuI	RGATCY	2 cut(s)	2345, 4331
PvuII	CAGCTG	2 cut(s)	2484, 2927
SacI	GAGCTC	3 cut(s)	1052, 1419, 3408
SacII	CCGCGG	1 cut(s)	326
SalI	GTCGAC	1 cut(s)	49
SapI	GCTCTTC	1 cut(s)	4139
Sau96I	GGNCC	9 cut(s)	333, 1582, 2440, 2730, 3309, 3837, 4076, 4265, 5382
ScaI	AGTACT	2 cut(s)	2085, 4613
ScrFI	CCNGG	9 cut(s)	101, 125, 143, 271, 2249, 2300, 3307, 4440, 4637
SduI	GDGCHC	9 cut(s)	20, 412, 1052, 1419, 1799, 2151, 3408, 5042, 5280
SfaNI	GCATC	6 cut(s)	1247, 1416, 2845, 3133, 4555, 4807
SfcI	CTRYAG	5 cut(s)	29, 1577, 2543, 2947, 5358
SfoI	GGCGCC	1 cut(s)	181
Sfr303I	CCGCGG	1 cut(s)	326
SgrBI	CCGCGG	1 cut(s)	326
SinI	GGWCC	4 cut(s)	333, 2730, 4076, 5382
SmlI	CTYRAG	2 cut(s)	4793, 5400
SmoI	CTYRAG	2 cut(s)	4793, 5400
SpeI	ACTAGT	1 cut(s)	1294
SspDI	GGCGCC	1 cut(s)	179
SspI	AATATT	1 cut(s)	5378
SspMI	CTAG	8 cut(s)	1295, 1811, 2087, 2472, 2888, 3135, 3941, 4223
SstI	GAGCTC	3 cut(s)	1052, 1419, 3408
StyD4I	CCNGG	9 cut(s)	99, 123, 141, 269, 2247, 2298, 3305, 4438, 4635
StyI	CCWWGG	4 cut(s)	1875, 2887, 3940, 5101
TaiI	ACGT	5 cut(s)	283, 1178, 2469, 2768, 5026
TaqI	TCGA	8 cut(s)	50, 204, 393, 831, 1052, 2494, 3420, 4450
TaqII	GACCGA	1 cut(s)	4064
TatI	WGTACW	5 cut(s)	149, 1687, 2083, 4611, 4743
TauI	GCSGC	7 cut(s)	78, 185, 188, 241, 325, 328, 2028
TseFI	GTSAC	7 cut(s)	1249, 1589, 1766, 2467, 2954, 4917, 5364
Tsp45I	GTSAC	7 cut(s)	1249, 1589, 1766, 2467, 2954, 4917, 5364
TspGWI	ACGGA	1 cut(s)	2683
VpaK11BI	GGWCC	4 cut(s)	333, 2730, 4076, 5382
VspI	ATTAAT	1 cut(s)	4599
XapI	RAATTY	5 cut(s)	482, 1622, 3195, 3634, 5406
XceI	RCATGY	6 cut(s)	2147, 3727, 3893, 4679, 4736, 4750
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2306
XmaJI	CCTAGG	2 cut(s)	2887, 3940
XmiI	GTMKAC	1 cut(s)	50
XmnI	GAANNNNTTC	5 cut(s)	1127, 2814, 3590, 3602, 3702
XspI	CTAG	8 cut(s)	1295, 1811, 2087, 2472, 2888, 3135, 3941, 4223
ZraI	GACGTC	1 cut(s)	281
ZrmI	AGTACT	2 cut(s)	2085, 4613
Zsp2I	ATGCAT	1 cut(s)	1240

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.