Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0013983

Physical Location & Seq

Genomic Coordinates Forward (+)

56980136 .. 56988670

Gene Structure 8535 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G032720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3147 bp

ATGGCCTCTACTACTACTACTAGTCTCCAAATCCCCACTTTCCTCTCCCTCAAACCACTCCCTCAACGCCCCTCCTTCCCCTTCTCCAACCACCCCACCTCCTTCTCCACCTCCTCCTCCCGCCCTCCACGCCGTCGTCTCACTCTCTCTCCCGCCCCCATTGCCGCCTCTCTCAAGGAGAATCTGGGCCGGATTGGAAAGACTTGGAGCGACGTGACTAGCTTGAACAGCTGGGTTGTTCGAGACTACTACCGCCTTGTCAACTCTGTCAACTCTTTTGAGCCACAGCTACAGAGGCTCACTGATGACCAGCTTACTGCTAAAACTGCCGAGTTTCGGCGAAGATTGGGACAAGGAGAGACACTTGCGGATATTCAAGCCGAGGCTTTTGCTGTTGTTCGTGAAGCTGCGAAACGGAAGCTTGGTATGCGTCATTTTGATGTTCAGATTATTGGTGGGGCAGTGCTTCATGATGGGTCCATTGCAGAAATGAAGACGGGAGAGGGAAAAACGTTGGTTTCCACATTAGCAGCATATCTTAATGCCCTGACTGGTGATGGTGTTCATGGTATGTTTTTCGATGCTGAGTGGATGGGTCGTGTTCATCGCTTCCTAGGTCTTTCAGTGGGTCTTATTCAGAGAGGGATGACAGCTGACAAGAGGAGGTCAAACTATAGATGTGACATAACATACACCAATAATTCAGAACTTGGGTTTGACTATCTACGAGACAATCTTGCCGGAAACAGTGGAGAACTTGTTATGAGATCGCCAAAGCCATTTCATTTTGCAATAGTTGATGAAGTTGACTCAGTTCTTATTGATGAAGGAAGAAATCCATTGCTAATTAGTGGCGAGGCTAGTAAAGATGCTGCTCGCTATCCTGTTGCTGCTAAAGTGGCTGAGTTGCTTGTGCGAGGAATTCATTACAATGTAGAACTTAAAGATTACTCAGTTGAGTTGACTGAAGAAGGAATAGCTCTTGCTGAGATGGCTCTTGAAACAAATGACTTATGGGATGAAAATGATCCCTGGGCCAGATTTGTGATGAATGCGTTGAAAGCTAAAGAGTTCTATCGGAAGGATGTGCAATATATTGTCAGAAATGGGAAGGCTCTCATAATCAATGAGCTCACTGGCAGAGTCGAAGATAAGAGAAGGTGGTCTGAGGGTATTCATCAAGCTGTGGAGGGAAAAGAAGGTTTGAAGATTCAGGCTGATTCGGTTGTTATCGCACAAATCACATATCAATCTCTGTTCAAGCTCTACCCAAAGCTATCAGGGATGACTGGCACCGCAAAAACTGAAGAGAAAGAGTTCTTGAAAATGTTCCAAACACCAGTTATTGAAGTACCAACAAATTTGCCAAACATTCGTAATGATTTACCTATTCAAGCTTTTGCGACTGCTCAGGGGAAATGGGAATATGTTCGTCAAGAAGTGGAATACATGTTCAGGCAGGGGCGTCCTGTTTTGGTTGGCACCACCAGTGTTGAGTATTCTGAACACCTGTCTGATCTGTTGAAGGAACATAACATCCCACACAATGTTCTAAATGCACGGCCCAAGTATGCTGCAAGGGAGGCTGAAATTGTTGCCCAAGCTGGACGAAAACATGCCATTACTCTTTCTACTAATATGGCTGGCAGAGGCACTGACATAATCCTAGGGGGCAATCCTAAAATGCTTGCAAAAGAAATCATAGAAGACAGTTTGATTTCATTCCTGACACGAGAAGCTCCTAACATTGACATAGATGGAGAGGCAATTTCACAAAAGGTATTGTCAAAGATTAAGGTCGGTCCATCATCATTAGCTTTGCTGGCTAAGACAGCGTTAATGGCTAAATATGTTGGCAAAAATGAAGGGAAGAGCTGGACATATAAGGAGGCAAAATCCATGATATCAGAGTCTGTGGAAATGAGTCAATCAAGAGATTTGAAAGAGCTAGAGAGGCTTGTTGATGAGCAGTCTGAGATATACCCTCTTGGCCCTACAATAGCACTTGCTTATCTGTCAGTTTTGAAGGATTGCGAGGTACACTGTTTCAAAGAAGGGTCTGAAGTGAAAAGACTTGGGGGACTTCATGTGATTGGAACATCTTTGCACGAGTCTCGTAGAATAGATAACCAGCTTCGTGGCAGAGCAGGAAGGCAAGGTGATCCAGGATCAACTCGATTTATGGTGAGCTTGCAGGATGAGATGTTTCAAAAGTTTAATTTTGATACTGAGTGGGCTGTGAGACTTATTTCAAAAATTACTGATGATGAGGATGTACCAATTGAAGGTGATGCCATTGTGAGACAGCTCCTGTCCCTGCAAATTAATGCTGAAAAGTACTTTTTTGGCATAAGAAAGAGCCTGGTTGAGTTTGATGAAGTATTAGAGGTGCAGAGAAAGCATGTTTATGAACTTCGGCAGTCAATTTTAACTGGTGATAATGAAAGTTGTTCCCAGCATGTATTCCAGTACATGCAAGCAGTGGTAGATGAAATTGTCTTTAAAAATGTTGACACCCTCAAGCATCCAAGAAAATGGAGTTTAAATAAGCTTTTGAAGGAGTTTATGGCAATTGCTGGGAAACTATTGGATGATTCATTTGCAGGAATTACTGAGGAAGCTTTGTTAGAATCTCTTGCGCAGTCACATGAACTGAACTTTAAAGATATTGATGACATTCACCTTCCAAACTTGCCAATACCTCCAAATGCCTTCAGAGGTATCCGCAAGAAGAACTCTTCATTGAAACGTTGGCTTGCTATATGCTCGGATGATTTGACCAAGAATGGAAGGTACCATGCTACTACTAACCTCCTCCGCAAGTACCTTGGAGACTATTTGATTGCTTCATATTTGGATGTTGTTCAAGACTCTGGTTATGATGATACATATGTGAAGGAAGTTGAAAGAGCAGTTATTGTGAAAACCCTTGATTGTTTCTGGAGGGATCACCTTGTAAACATGAACAGGCTAAGTTCAGCGGTGAATGTAAGGAGTTTTGGGCACAGGAATCCTCTTGAAGAATACAAGATTGATGGTTGTCGTTTTTTCATATCCATGCTTAGTGCAACACGAAGGTTGACTGTAGAGTCACTTTTGCAATATTGGTCCTCCCCAATGGAGTCTCAAGAAATTTTTGTATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0006996 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0009657 GO:0009658 GO:0009987 GO:0010109 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0019222 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0031323 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW WD40 zf-RING_UBOX

Protein Analysis

1048

Amino Acids

118.31

Weight (kDa)

6.5

Isoelectric Point (pI)

37.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	78 - 440	4.9e-101	SecA DEAD-like domain
SecA_PP_bind	PF01043	291 - 397	5.3e-29	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	456 - 743	4.6e-66	SecA P-loop domain
SecA_SW	PF07516	760 - 1034	3.8e-42	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21650 AT1G21650 AT1G21650
fragaria_vesca	FvH4_2g27750
malus_domestica	MD00G1100600.v1.1
prunus_persica	Prupe.1G490400_v2.0.a1
pyrus_communis	pycom15g30570
rosa_chinensis	RchiOBHm_Chr6g0296621
rosa_laevigata	RLG00000011676
rosa_multiflora	Rmu_sc0001741.1_g000019
rosa_roxburghii	Rroxscaffold_7G00171330 Rroxscaffold_7G00178770
rosa_rugosa	Rorug06G0263700
rosa_samantha	Rh6AG375100 Rh6CG388700
rosa_wichuraiana	Rw6G032720 Rw6G032810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3088
Acc16I	TGCGCA	1 cut(s)	2640
Acc65I	GGTACC	1 cut(s)	2793
AccB1I	GGYRCC	3 cut(s)	1292, 1481, 2793
AciI	CCGC	9 cut(s)	121, 153, 165, 253, 368, 1296, 2725, 2818, 2981
AclI	AACGTT	2 cut(s)	512, 2749
AclWI	GGATC	4 cut(s)	1022, 2156, 2176, 2955
AcsI	RAATTY	3 cut(s)	921, 1360, 3132
AcuI	CTGAAG	4 cut(s)	987, 1326, 2082, 2698
AcyI	GRCGYC	1 cut(s)	1465
AfaI	GTAC	7 cut(s)	1353, 2040, 2277, 2339, 2471, 2795, 2825
AfiI	CCNNNNNNNGG	2 cut(s)	336, 2285
AflIII	ACRYGT	1 cut(s)	1449
AhlI	ACTAGT	1 cut(s)	20
AjiI	CACGTC	1 cut(s)	214
AjnI	CCWGG	3 cut(s)	1031, 2164, 2361
AloI	GAACNNNNNNTCC	4 cut(s)	1436, 1468, 1521, 1553
Alw21I	GWGCWC	1 cut(s)	1134
AlwI	GGATC	4 cut(s)	1022, 2156, 2176, 2955
AlwNI	CAGNNNCTG	2 cut(s)	1913, 2311
AoxI	GGCC	5 cut(s)	3, 187, 1035, 1562, 1990
ApeKI	GCWGC	5 cut(s)	407, 530, 872, 890, 1574
ApoI	RAATTY	3 cut(s)	921, 1360, 3132
AseI	ATTAAT	1 cut(s)	2325
Asp700I	GAANNNNTTC	3 cut(s)	1316, 1328, 1428
Asp718I	GGTACC	1 cut(s)	2793
AspA2I	CCTAGG	2 cut(s)	613, 1666
AspLEI	GCGC	1 cut(s)	2641
AspS9I	GGNCC	7 cut(s)	187, 477, 1035, 1563, 1802, 1991, 3108
AsuHPI	GGTGA	8 cut(s)	566, 2171, 2197, 2300, 2447, 2672, 2942, 2995
AvaII	GGWCC	3 cut(s)	477, 1802, 3108
AvrII	CCTAGG	2 cut(s)	613, 1666
BaeGI	GKGCMC	1 cut(s)	3006
BaeI	ACNNNNGTAYC	2 cut(s)	2877, 2910
BanI	GGYRCC	3 cut(s)	1292, 1481, 2793
BanII	GRGCYC	1 cut(s)	1134
BauI	CACGAG	2 cut(s)	1731, 2108
BbsI	GAAGAC	2 cut(s)	500, 1713
Bbv12I	GWGCWC	1 cut(s)	1134
BbvI	GCAGC	5 cut(s)	394, 542, 859, 877, 1561
BccI	CCATC	7 cut(s)	467, 551, 586, 985, 1751, 1813, 3029
BceAI	ACGGC	2 cut(s)	117, 1577
BcgI	CGANNNNNNTGC	4 cut(s)	371, 405, 2096, 2130
BciT130I	CCWGG	3 cut(s)	1033, 2166, 2363
BciVI	GTATCC	1 cut(s)	2732
BcuI	ACTAGT	1 cut(s)	20
BfaI	CTAG	6 cut(s)	21, 219, 614, 861, 1667, 1949
BfmI	CTRYAG	3 cut(s)	290, 673, 3084
BfuI	GTATCC	1 cut(s)	2732
BisI	GCNGC	6 cut(s)	165, 408, 531, 873, 891, 1575
BlnI	CCTAGG	2 cut(s)	613, 1666
BlsI	GCNGC	6 cut(s)	166, 409, 532, 874, 892, 1576
BmcAI	AGTACT	1 cut(s)	2339
Bme1390I	CCNGG	3 cut(s)	1033, 2166, 2363
Bme18I	GGWCC	3 cut(s)	477, 1802, 3108
BmgBI	CACGTC	1 cut(s)	214
BmgT120I	GGNCC	7 cut(s)	187, 477, 1035, 1563, 1802, 1991, 3108
BmiI	GGNNCC	4 cut(s)	478, 1294, 1483, 2795
BmrFI	CCNGG	3 cut(s)	1033, 2166, 2363
BmsI	GCATC	4 cut(s)	571, 859, 2281, 2533
BpiI	GAAGAC	2 cut(s)	500, 1713
BpmI	CTGGAG	1 cut(s)	2962
Bpu10I	CCTNAGC	1 cut(s)	1410
BpuEI	CTTGAG	3 cut(s)	158, 2504, 3111
BsaHI	GRCGYC	1 cut(s)	1465
BsaJI	CCNNGG	6 cut(s)	381, 613, 1031, 1032, 1666, 2827
BsaXI	ACNNNNNCTCC	6 cut(s)	83, 113, 133, 163, 744, 774
Bsc4I	CCNNNNNNNGG	2 cut(s)	336, 2285
Bse1I	ACTGG	7 cut(s)	556, 1141, 1294, 1340, 1488, 2437, 2467
Bse3DI	GCAATG	3 cut(s)	159, 480, 839
BseBI	CCWGG	3 cut(s)	1033, 2166, 2363
BseDI	CCNNGG	6 cut(s)	381, 613, 1031, 1032, 1666, 2827
BseLI	CCNNNNNNNGG	2 cut(s)	336, 2285
BseMI	GCAATG	3 cut(s)	159, 480, 839
BseNI	ACTGG	7 cut(s)	556, 1141, 1294, 1340, 1488, 2437, 2467
BseRI	GAGGAG	4 cut(s)	103, 106, 676, 2804
BseSI	GKGCMC	1 cut(s)	3006
BseXI	GCAGC	5 cut(s)	394, 542, 859, 877, 1561
BseYI	CCCAGC	3 cut(s)	231, 2454, 2576
BsgI	GTGCAG	1 cut(s)	2411
BshFI	GGCC	5 cut(s)	5, 189, 1037, 1564, 1992
BshNI	GGYRCC	3 cut(s)	1292, 1481, 2793
BsiHKAI	GWGCWC	1 cut(s)	1134
BsiSI	CCGG	2 cut(s)	190, 741
BslFI	GGGAC	3 cut(s)	363, 2094, 2299
BslI	CCNNNNNNNGG	2 cut(s)	336, 2285
BsmBI	CGTCTC	1 cut(s)	143
BsmFI	GGGAC	3 cut(s)	363, 2094, 2299
BsmI	GAATGC	1 cut(s)	1057
BsnI	GGCC	5 cut(s)	5, 189, 1037, 1564, 1992
Bsp1286I	GDGCHC	2 cut(s)	1134, 3006
Bsp143I	GATC	6 cut(s)	767, 1027, 1516, 2161, 2168, 2947
BspACI	CCGC	9 cut(s)	121, 153, 165, 253, 368, 1296, 2725, 2818, 2981
BspANI	GGCC	5 cut(s)	5, 189, 1037, 1564, 1992
BspHI	TCATGA	2 cut(s)	469, 3143
BspLI	GGNNCC	4 cut(s)	478, 1294, 1483, 2795
BspPI	GGATC	4 cut(s)	1022, 2156, 2176, 2955
BspQI	GCTCTTC	1 cut(s)	1865
BspT107I	GGYRCC	3 cut(s)	1292, 1481, 2793
BsrDI	GCAATG	3 cut(s)	159, 480, 839
BsrI	ACTGG	7 cut(s)	556, 1141, 1294, 1340, 1488, 2437, 2467
BssECI	CCNNGG	6 cut(s)	381, 613, 1031, 1032, 1666, 2827
BssMI	GATC	6 cut(s)	767, 1027, 1516, 2161, 2168, 2947
BssNI	GRCGYC	1 cut(s)	1465
BssSI	CACGAG	2 cut(s)	1731, 2108
BssT1I	CCWWGG	3 cut(s)	613, 1666, 2827
Bst2BI	CACGAG	2 cut(s)	1731, 2108
Bst2UI	CCWGG	3 cut(s)	1033, 2166, 2363
Bst4CI	ACNGT	4 cut(s)	749, 1712, 2045, 3085
Bst6I	CTCTTC	3 cut(s)	1302, 1865, 2743
BstACI	GRCGYC	1 cut(s)	1465
BstC8I	GCNNGC	7 cut(s)	877, 1645, 1689, 1824, 2192, 2478, 2757
BstHHI	GCGC	1 cut(s)	2641
BstKTI	GATC	6 cut(s)	770, 1030, 1519, 2164, 2171, 2950
BstMBI	GATC	6 cut(s)	767, 1027, 1516, 2161, 2168, 2947
BstNI	CCWGG	3 cut(s)	1033, 2166, 2363
BstNSI	RCATGY	5 cut(s)	1453, 1619, 2405, 2462, 2476
BstSCI	CCNGG	3 cut(s)	1031, 2164, 2361
BstSFI	CTRYAG	3 cut(s)	290, 673, 3084
BstSLI	GKGCMC	1 cut(s)	3006
BstV1I	GCAGC	5 cut(s)	394, 542, 859, 877, 1561
BstV2I	GAAGAC	2 cut(s)	500, 1713
BstXI	CCANNNNNNTGG	2 cut(s)	2138, 2535
BsuI	GTATCC	1 cut(s)	2732
BsuRI	GGCC	5 cut(s)	5, 189, 1037, 1564, 1992
BtgZI	GCGATG	1 cut(s)	590
BtrI	CACGTC	1 cut(s)	214
BtsI	GCAGTG	2 cut(s)	468, 2487
BtsIMutI	CAGTG	9 cut(s)	300, 468, 630, 754, 1134, 1495, 1653, 2041, 2487
Cac8I	GCNNGC	7 cut(s)	877, 1645, 1689, 1824, 2192, 2478, 2757
CaiI	CAGNNNCTG	2 cut(s)	1913, 2311
CciI	TCATGA	2 cut(s)	469, 3143
CfoI	GCGC	1 cut(s)	2641
Cfr13I	GGNCC	7 cut(s)	187, 477, 1035, 1563, 1802, 1991, 3108
CseI	GACGC	2 cut(s)	419, 1454
Csp6I	GTAC	7 cut(s)	1352, 2039, 2276, 2338, 2470, 2794, 2824
CspCI	CAANNNNNGTGG	2 cut(s)	1474, 1509
CviQI	GTAC	7 cut(s)	1352, 2039, 2276, 2338, 2470, 2794, 2824
DpnI	GATC	6 cut(s)	769, 1029, 1518, 2163, 2170, 2949
DpnII	GATC	6 cut(s)	767, 1027, 1516, 2161, 2168, 2947
DraI	TTTAAA	3 cut(s)	2503, 2544, 2662
DrdI	GACNNNNNNGTC	1 cut(s)	3088
DseDI	GACNNNNNNGTC	1 cut(s)	3088
Eam1104I	CTCTTC	3 cut(s)	1302, 1865, 2743
EarI	CTCTTC	3 cut(s)	1302, 1865, 2743
Ecl136II	GAGCTC	1 cut(s)	1132
Eco130I	CCWWGG	3 cut(s)	613, 1666, 2827
Eco24I	GRGCYC	1 cut(s)	1134
Eco32I	GATATC	1 cut(s)	1905
Eco47I	GGWCC	3 cut(s)	477, 1802, 3108
Eco53kI	GAGCTC	1 cut(s)	1132
Eco57I	CTGAAG	4 cut(s)	987, 1326, 2082, 2698
EcoICRI	GAGCTC	1 cut(s)	1132
EcoRI	GAATTC	1 cut(s)	921
EcoRII	CCWGG	3 cut(s)	1031, 2164, 2361
EcoRV	GATATC	1 cut(s)	1905
EcoT14I	CCWWGG	3 cut(s)	613, 1666, 2827
EcoT38I	GRGCYC	1 cut(s)	1134
ErhI	CCWWGG	3 cut(s)	613, 1666, 2827
Esp3I	CGTCTC	1 cut(s)	143
FaqI	GGGAC	3 cut(s)	363, 2094, 2299
FauI	CCCGC	2 cut(s)	128, 160
FauNDI	CATATG	1 cut(s)	2890
Fnu4HI	GCNGC	6 cut(s)	165, 408, 531, 873, 891, 1575
FriOI	GRGCYC	1 cut(s)	1134
Fsp4HI	GCNGC	6 cut(s)	165, 408, 531, 873, 891, 1575
FspBI	CTAG	6 cut(s)	21, 219, 614, 861, 1667, 1949
FspI	TGCGCA	1 cut(s)	2640
GlaI	GCGC	1 cut(s)	2640
GluI	GCNGC	6 cut(s)	165, 408, 531, 873, 891, 1575
GsaI	CCCAGC	3 cut(s)	235, 2458, 2580
GsuI	CTGGAG	1 cut(s)	2962
HaeIII	GGCC	5 cut(s)	5, 189, 1037, 1564, 1992
HapII	CCGG	2 cut(s)	190, 741
HgaI	GACGC	2 cut(s)	419, 1454
HhaI	GCGC	1 cut(s)	2641
Hin1I	GRCGYC	1 cut(s)	1465
Hin6I	GCGC	1 cut(s)	2639
HinP1I	GCGC	1 cut(s)	2639
HincII	GTYRAC	6 cut(s)	262, 271, 808, 963, 2512, 3081
HindII	GTYRAC	6 cut(s)	262, 271, 808, 963, 2512, 3081
HindIII	AAGCTT	4 cut(s)	419, 1395, 2549, 2619
HpaII	CCGG	2 cut(s)	190, 741
HphI	GGTGA	8 cut(s)	566, 2171, 2197, 2300, 2447, 2672, 2942, 2995
Hpy166II	GTNNAC	8 cut(s)	262, 271, 808, 963, 2041, 2512, 2959, 3081
Hpy8I	GTNNAC	8 cut(s)	262, 271, 808, 963, 2041, 2512, 2959, 3081
Hpy99I	CGWCG	2 cut(s)	138, 215
HpyCH4III	ACNGT	4 cut(s)	749, 1712, 2045, 3085
HpyCH4IV	ACGT	3 cut(s)	213, 512, 2749
HpySE526I	ACGT	3 cut(s)	213, 512, 2749
Hsp92I	GRCGYC	1 cut(s)	1465
HspAI	GCGC	1 cut(s)	2639
KpnI	GGTACC	1 cut(s)	2797
Kzo9I	GATC	6 cut(s)	767, 1027, 1516, 2161, 2168, 2947
LguI	GCTCTTC	1 cut(s)	1865
LmnI	GCTCC	3 cut(s)	207, 1744, 2313
Lsp1109I	GCAGC	5 cut(s)	394, 542, 859, 877, 1561
LweI	GCATC	4 cut(s)	571, 859, 2281, 2533
MaeI	CTAG	6 cut(s)	21, 219, 614, 861, 1667, 1949
MaeII	ACGT	3 cut(s)	213, 512, 2749
MaeIII	GTNAC	4 cut(s)	214, 680, 2643, 3090
MalI	GATC	6 cut(s)	769, 1029, 1518, 2163, 2170, 2949
MboI	GATC	6 cut(s)	767, 1027, 1516, 2161, 2168, 2947
MfeI	CAATTG	2 cut(s)	2280, 2571
MhlI	GDGCHC	2 cut(s)	1134, 3006
MlyI	GAGTC	8 cut(s)	803, 1152, 1919, 1933, 2120, 2864, 3098, 3131
MmeI	TCCRAC	1 cut(s)	111
MroXI	GAANNNNTTC	3 cut(s)	1316, 1328, 1428
MslI	CAYNNNNRTG	4 cut(s)	438, 930, 2406, 3056
MspA1I	CMGCKG	3 cut(s)	231, 653, 2981
MspI	CCGG	2 cut(s)	190, 741
MspR9I	CCNGG	3 cut(s)	1033, 2166, 2363
MunI	CAATTG	2 cut(s)	2280, 2571
Mva1269I	GAATGC	1 cut(s)	1057
MvaI	CCWGG	3 cut(s)	1033, 2166, 2363
NdeI	CATATG	1 cut(s)	2890
NdeII	GATC	6 cut(s)	767, 1027, 1516, 2161, 2168, 2947
NlaIV	GGNNCC	4 cut(s)	478, 1294, 1483, 2795
NmeAIII	GCCGAG	2 cut(s)	355, 406
NmuCI	GTSAC	4 cut(s)	214, 680, 2643, 3090
NsbI	TGCGCA	1 cut(s)	2640
NspI	RCATGY	5 cut(s)	1453, 1619, 2405, 2462, 2476
PagI	TCATGA	2 cut(s)	469, 3143
PasI	CCCWGGG	1 cut(s)	1032
PciI	ACATGT	1 cut(s)	1449
PciSI	GCTCTTC	1 cut(s)	1865
PctI	GAATGC	1 cut(s)	1057
PdmI	GAANNNNTTC	3 cut(s)	1316, 1328, 1428
PfeI	GAWTC	6 cut(s)	181, 1210, 1220, 2594, 2630, 3010
PfoI	TCCNGGA	1 cut(s)	2164
PkrI	GCNGC	6 cut(s)	166, 409, 532, 874, 892, 1576
PleI	GAGTC	8 cut(s)	803, 1151, 1918, 1932, 2119, 2864, 3097, 3130
PpsI	GAGTC	8 cut(s)	803, 1151, 1918, 1932, 2119, 2864, 3097, 3130
PscI	ACATGT	1 cut(s)	1449
PshBI	ATTAAT	1 cut(s)	2325
Psp124BI	GAGCTC	1 cut(s)	1134
Psp1406I	AACGTT	2 cut(s)	512, 2749
Psp6I	CCWGG	3 cut(s)	1031, 2164, 2361
PspFI	CCCAGC	3 cut(s)	231, 2454, 2576
PspGI	CCWGG	3 cut(s)	1031, 2164, 2361
PspN4I	GGNNCC	4 cut(s)	478, 1294, 1483, 2795
PspPI	GGNCC	7 cut(s)	187, 477, 1035, 1563, 1802, 1991, 3108
PstNI	CAGNNNCTG	2 cut(s)	1913, 2311
PvuII	CAGCTG	2 cut(s)	231, 653
RsaI	GTAC	7 cut(s)	1353, 2040, 2277, 2339, 2471, 2795, 2825
RsaNI	GTAC	7 cut(s)	1352, 2039, 2276, 2338, 2470, 2794, 2824
RseI	CAYNNNNRTG	4 cut(s)	438, 930, 2406, 3056
SacI	GAGCTC	1 cut(s)	1134
SapI	GCTCTTC	1 cut(s)	1865
SatI	GCNGC	6 cut(s)	165, 408, 531, 873, 891, 1575
Sau3AI	GATC	6 cut(s)	767, 1027, 1516, 2161, 2168, 2947
Sau96I	GGNCC	7 cut(s)	187, 477, 1035, 1563, 1802, 1991, 3108
ScaI	AGTACT	1 cut(s)	2339
SchI	GAGTC	8 cut(s)	803, 1152, 1919, 1933, 2120, 2864, 3098, 3131
ScrFI	CCNGG	3 cut(s)	1033, 2166, 2363
SduI	GDGCHC	2 cut(s)	1134, 3006
SfaNI	GCATC	4 cut(s)	571, 859, 2281, 2533
SfcI	CTRYAG	3 cut(s)	290, 673, 3084
SinI	GGWCC	3 cut(s)	477, 1802, 3108
SmiMI	CAYNNNNRTG	4 cut(s)	438, 930, 2406, 3056
SmlI	CTYRAG	3 cut(s)	173, 2519, 3126
SmoI	CTYRAG	3 cut(s)	173, 2519, 3126
SpeI	ACTAGT	1 cut(s)	20
SsiI	CCGC	9 cut(s)	121, 153, 165, 253, 368, 1296, 2725, 2818, 2981
SspI	AATATT	1 cut(s)	3104
SspMI	CTAG	6 cut(s)	21, 219, 614, 861, 1667, 1949
SstI	GAGCTC	1 cut(s)	1134
StyD4I	CCNGG	3 cut(s)	1031, 2164, 2361
StyI	CCWWGG	3 cut(s)	613, 1666, 2827
TaaI	ACNGT	4 cut(s)	749, 1712, 2045, 3085
TaiI	ACGT	3 cut(s)	216, 515, 2752
TaqI	TCGA	4 cut(s)	241, 579, 1146, 2176
TaqII	GACCGA	1 cut(s)	1790
TatI	WGTACW	2 cut(s)	2337, 2469
TauI	GCSGC	1 cut(s)	167
TfiI	GAWTC	6 cut(s)	181, 1210, 1220, 2594, 2630, 3010
TscAI	CASTG	9 cut(s)	307, 468, 630, 754, 1141, 1495, 1660, 2048, 2487
TseFI	GTSAC	4 cut(s)	214, 680, 2643, 3090
TseI	GCWGC	5 cut(s)	407, 530, 872, 890, 1574
Tsp45I	GTSAC	4 cut(s)	214, 680, 2643, 3090
TspGWI	ACGGA	1 cut(s)	430
TspRI	CASTG	9 cut(s)	307, 468, 630, 754, 1141, 1495, 1660, 2048, 2487
VpaK11BI	GGWCC	3 cut(s)	477, 1802, 3108
VspI	ATTAAT	1 cut(s)	2325
XapI	RAATTY	3 cut(s)	921, 1360, 3132
XceI	RCATGY	5 cut(s)	1453, 1619, 2405, 2462, 2476
XmaJI	CCTAGG	2 cut(s)	613, 1666
XmnI	GAANNNNTTC	3 cut(s)	1316, 1328, 1428
XspI	CTAG	6 cut(s)	21, 219, 614, 861, 1667, 1949
ZrmI	AGTACT	1 cut(s)	2339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.