AT1G22060

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003440
Physical Location & Seq
Reverse (-)
7773063 .. 7781539
8477 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G22060.2

Sequence Viewer

Length: 6000 bp
ATGTCGAGACTGGCTAAATGGAAGCTGGAGAAAGCCAAAGTCAAGGTCGTCTTTCGATTGCAGTTTCACGCTACCCATGTTCCACAAGCGGGTTGGGACAAACTATTTATATCTTTCATTCCCGCTGACTCCGTAAAAGCTACAGCAAAGACAACTAAAGCACTTGTGAGAAATGGAACTTGCAAGTGGGGGGATCCTATCTATGAAACTACAAGGCTGCTTCAGGATACTAGGACCAAGCAATTTGATGAAAAACTTTATAAGATTGTTGTCGCTATGGGTACTTCTAGATCTAGTATACTTGGAGAGGCTATGATCAACCTTGCTGAATATGCTGATGCCTTAAAACCTTTTGCTGTGATATTGCCCTTGCAAGGCTGTGATCCAGGAGCTATCTTACATGTCACTATACAGCTGTTAACCTCAAAAACTGGTTTCAGAGAGTTCGAGCAGCAAAGAGAAATCAGTGAGAGGGGACCATCAACGACCCCTGACCATAGCAGTCCTGACGAATCTTCTCGTTGCAGGATTTCACCTTCTGATGAGACTCTTAGTCATGTTGATAAGACAAATATAAGAGGGAGTTTCAAAGAAAAGTTCCGAGACAATTCTTTAGTGGAAGAAACAGTGGGGCTAAATGACCTTGATTCAGGATTGGGTTTTGATGTCTCTTCAAACACATCAGGGAGCCTAAACGCTGAGAAACATGATATATCCAGCATAAACGAAGTTGACAGCCTCAAAAGTGTGGTATCTGGAGATCTGAGTGGACTTGCTCAGAGCCCGCAGAAAGAGAAAGACAGTCTTGGGTGGCAACATGGCTGGGGGTCAGACTATCTTGGTAAGAATAGCGATTTGGGCAATGCAATAGAAGACAACAATAAGCTTAAAGGATTCTTGGAAGACATGGAATCATCCATTAATGAGATTAAGATTGAAGTGAGTTCGCTGCAGTGCCATGCAGATGATATAGGTTCCAAAGCTCAAGATTTTTCTCAAATACTTATATCAGAGATTGGTTCTGGGGATCATTTGGTCAGAGAAGTATCTGTGCTGAAATCAGAGTGTTCAAAGTTAAAAGAAGAGATGGAGCGATTACGTAACGTCAAATCACACGTTCTATTTAATAGCAAAGATCAAGATAATGTTCCCCACAGTCTACAACTAAGATGGCTGCAGGGACTTTTAGTTGTAGAGGATAACATAAGAGAAATTCAAAACAAAGTATGCTATGGCTACCATGACAGAGACTTAAGATTGTTCCTCTCAGACTTTGAGTCTCTGCTCGGAGTTCTACAGGATTTTAAAACGCAGATTGAGCAGCCTATATCTCACTTCAGCACTGTACCATCTGAGAAAATCATCATGACTGATAGTAAAGAGAGAGGCCTTTCGAAAGCAAAGCATTTTGTATCAGGCTCTGAGGTTGACACTGATATTTATCAACCAGAACTTGATCCGCTTCAATATCTTGGTATGCCTGATCTTACTTCTCGTGAACCCAATTCAGCAGATTCTGTTAGTGCCATGAGAGATAAAATACTTGAGCTTGTACGAGGGCTGGACGAGTCTAAAGCTGAACGTGATAGCCTTACCAAGAAAATGGATCAGATGGAATGCTATTACGAATCTCTGGTCCAGGAGCTTGAGGAAACTCAAAGGCAGTTGCTAGTAGAGTTACAGAGTTTAAGGACTGAACATTCCACGTGCCTCTATAGTATCTCTGGCGCTAAGGCGGAGATGGAAACGTTGCGTCACGACATGAATGAGCAAACACTAAGGTTTTCTGAGGAGAAGAAAACTTTGGATTCTTTTAATGAAGAACTGGATAAACGAGCTATGGCTGCAGAGGCAGCACTTAAAAGGGCCCGCTTGAATTATTCTATTGCTGTCAACCATTTGCAGAAGGATCTTGAATTGCTTTCATCTCAGGTTGTATCCATGTTTGAGACCAATGAGAATCTCATTAAGCAAGCCTTTCCAGAACCTCCACAAAGCTTTCACGAATGCATTCAATCAACAGATGATTCAATTTCTGAGAAACAGGACACTCGTGATGTAAAACTCATACAGTTTCAGAATGAGAAAAAGGGGATGAAGGAACGCCCTTTAAAAGGTGATATTATTCTTCTAGAAGATATGAAGAGATCTCTTCATGTGCAAGAAAGCTTGTATCAAAAAGTTGAAGAAGAACTCTATGAGATGCACTCAAGGAACTTATATCTGGAAGTATTCTCAAACATTCTACGAGAAACTTTTCTTGAAGCAAGTGTTGACATAAGGATCATGAAAGCGAAAATTGATGAACTTGGATGGCAGCTGGAGCTTTCAACAGAGGCCAAGGAAATATTGAAGCAGAGGCTGGATATTACTTTGGACGAGGTTTGCTCCCTGAAAGAGGAGAAAACCACATGCATTGCCAAATGGAATGCTGTAGCTTTGCAGAATCAAAGCTTAGAGGCAAATTTGCAGAATATTACTCACGAAAACCTCATCCTTCTGCAAAAGATTGATGAACTGGAATCTGTAGTGTTGGAGTCTAAGAGTTGGAAGACCAATTATGAGACATGCATTTGTGAGAAAAAAGAGCTTGCAGAGCTTATGGAGAAAGAAGCGGTGGAAAAAGCTCATTATCGGACAAGGCTTGCTACTGTGCAGGCTGAGTTTGATGCTGTGAGAGGTAAGTTTGATGATTTGGCTACTGCAAATGGAAATCTACAGCAGAATCTCAGTAGTCTGACGGACAAGTTGATAAACACGTTAGGCTGCTACAATGAAAAACTCGTTTCCTTGCCCCAGTGGGAAGGAGTGGATCTTGATTTTGAGTCCCATGATTTAACAGAGCAGCTTGATAAATTCTTGTGTAAGATATGTGAAAAGTGTTTCGTACTTATATCAGAGAATAATGGCCTGATGAAAGAAAAATCCATGACAGAGTCATATTTAAGGGCTGCTGAGTCTGATGTTATGGAGCTCAAGCAGATGCATGAGAATGATGTACAATGTATGGTCACTAAATTAGAAGCTTCCACTGCCCTCCTCCGGAGACTGCAGTTGGAAACCGAATCGGTCATGGATAAAATGAAGGTTATTACTGAAGATGAACAAAACTATGAGAGCCGTCACTTGGACCTTCTTTCTCGTCTTGACCATTTTGAGAATGAAATGCATCTACTTGTGTCCAAGAATGAGGGACTTGGTCAAGAGATTAGTGAATTAAGCTCTGTAGCAGTTGAACATGGAAGAACCAAGCTGCTTGTGGAAGAACTGGCTGAGGAAAAGAAACGTGTTCTGGTTTCACTTCAAGATAAATCTCAGGAAACTTTGGGTCTTGTGCGTGAGCTTGAAAATTTAAAGAAAACGTTTGACCACGAGTTGAGACTTGAAAGAAACTTGAGGCAGGAACTAGAAATTAAAATGCAAGATCTTACATCTGAGGTGATCGCCAAAAGTTCCAAATTGATGAGCTTTGATGAGCAAAGCTCTGAGTTGGTCCGTCTTAAGCAAATGGTTTCCGATCTGGAGCTGGAGAAGGCAACTCACACTCATCGTCTGACAAGATATGAAACATCTCTGAGAAGCTTGACTCGTGATTCATCATATATTTCCGATTTGGAATCTCAGATACTTGAAATGATGGAAATCTCAGTAGCAGCAGATATCCAGATTGTTTTCACAAGAACTGAATGGGAGACTTATGCTGACAAGCTCCACAAGGATCATTTTGAGGTACTGACTGCCTTCAATGACTCTCGTAATGTAGGAGCTCAGCATATGAATGCTAATATAAAGTTGTTGGCAGATCTCGACTCCCTAAAATCAGAATTGAAAATTGAGAGAAATTTGAGAAACAACTTAGACAGAAGAGTTGAAGAACTCACCTCTGAGTTAGATGAAAAACATTTGTTGTTAGAGAACTTTGATTTGCAGAAATCTCAAGTGGAACTTTTGGAGAAGATGGTAGCTGAGCTGGAATCTGAAAAGTCTTTTCAACGTTTGGAGTACGTTAGAAATGCTCATCGAGAGTCTTCTTTCATAGAAGAACTGTTTCAGTGTCTAATGGCTGCAGATGTTCAGCTTATTTTTACAAAGATCCAGTCTGATATCTGTATCAATGAGTTTGCAGAACAACTTAGCTGCTGCTCTAATAGCCACCTTGAGTTTCAGAAGAAGTACACTGATGTGGAATCTGCCCTTAACCATTGTCTAGTCAACGAAACGCGGTATATGGACGAAAACAATCAGTTATTGATTAATCTTGAAGTCTTAAAGTCTGAGCTAGAGTCTTCCATGGCTAAAAGTAGAGCTCTTGCTGATAGAAATGATGAAATGTCAGCTGAACTCGAAGAGCACGCAACTAGGGATGAGAATGCAGAAAGAAGTTACTCTGAGAGAAGCTTATGTGCTCCTGAGGTCGAGCAATTAAAATCTTTGCTGTTTGGGTATGAAGAAGAAATAGAAAATTTGACGGTGTTAAAAGCGGAGGCGGAAATTACAGTTGAGATACTAAAAGATAAACTAACTGGGCTTTGTGGGAAAGGTGCCAGTGAGCTTGAAACACTGAAAAATAGGTGCAGTGATCTCACTCAGAAGCTCTCTGAGCAGATTTTGAAAACAGAAGAGTTCAAAAGCATGTCCAATCATTTGAAAGAGCTTAAGGACAATGCTGAAGCAGAATGCAATCGTGCTCGTGAAAAGGCCGACTACAAAGCCCCGCTAACTCCACAGCAGGAGTCGTTGAGAATCATTTTCATCAAAGAACAGTATGACACCAAGCTGCAGGAACTTCAATATCAACTGACCATGTCCAAGAAGCATGGTGAGGAAATTCTGATGAAATTACAAGATGCCATTGATGAGAATGAAGCAAGGAAAAAGGCAGAATCCAGCCAATTAAAGAGATCTAAAGAGCTGGAGGGAAAAATATTGGAACTGGAGGCTGATCGACAATCTGTTATCTATGATAAACGTGAGAAAACCACGGCTTATGACATGATGAAGGCAGAGCTGGATTGCTCATTATTAAGCCTTGAGTGCTGCAAAGAAGAAAAACAGAAGCTTGAAGCTATTCTGCAACAATGCAAAGAGCAGAGTTTGAAAATGTCCAAAGAGCTAGAATCAAGGAGAGGATTAGTACAACGTTGCAGCTCTCAGAAAAATATTGAGATGGAAGAAAATGATAGATTAAATAGTGAGGTGTCAGAGTTGGCTGACAAAAATACAATTGCAGTCTCTTCAGGCGACTCGGTGAACAACGGGCAAAGGGAAGTTGCTTGTATTGATCCTACAGTGCGGATCATCAGTCCCAGGTCGATAATTCAGGGTACTATTCAATCGAGCAGTGTAAATGGAAACAGAGACCAATTACCTTCCGGAGAAGCAATGGCGCTGGACAAACGTGAAGAAAGCCTAGCTTTGATCAATGATAAATTCAGGGCAGAGACTTTAAGGTCTAGCATGGATCACCTAAACGATGAGCTAGAACGGATGAAGAATGAGAATCTACTTGAACCTCAAGATGATAATGATTCAGACACCAGATTTCCGGGTTTGGAACAGGAGCTGATGCAGTTGCGTCAGGCAAAGGAAGAACTTCAGAGTATCTTCCCTCTATCTCAAGAGAATTTCAGTTGCGGAAACGCATTGGAAAGGGTTCTTGCACTGGAAATAGAACTCGCTGAAGCATTGCGTGGAAAGAAAAAATCAACCACCCATTTCCAGAGCTCTTTCTTGAAGCAACACACCGATGACGAAGCAATCTTCCAGAGTTTCAGAGACATAAACAATCTAATTGAAGAAATGTTGGATACCAAAGGCCGGTATTCGTCTATGGAGACAGAGCTTAGGGAAATGCATGACCGTTATTCTCAGCTAAGCCTCAAGTTTGCAGAGGTTGAAGGAGAGAGACAAAAGCTGATGATGACTCTGAAGAACGTTAGGGCATCAAAGAAGGCTATGCTCTTGAACCGTTCATCATCAGCAACTCTTGGTGAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1999

Amino Acids

228.95

Weight (kDa)

5.01

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 141 7.8e-27 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 261
AasI GACNNNNNNGTC 1 cut(s) 5449
AccB1I GGYRCC 1 cut(s) 4541
AccI GTMKAC 2 cut(s) 298, 1159
AccII CGCG 1 cut(s) 4222
AccIII TCCGGA 2 cut(s) 3042, 5372
AclI AACGTT 5 cut(s) 1748, 3362, 3994, 5140, 5934
AcsI RAATTY 9 cut(s) 1212, 2464, 2855, 3349, 3841, 4462, 4827, 5429, 5623
AcuI CTGAAG 8 cut(s) 206, 1321, 3117, 4689, 5220, 5579, 5700, 5948
AcvI CACGTG 1 cut(s) 1707
AfiI CCNNNNNNNGG 7 cut(s) 89, 374, 2114, 2398, 3042, 3127, 5817
AflII CTTAAG 3 cut(s) 1252, 3500, 4655
AflIII ACRYGT 4 cut(s) 400, 1114, 2757, 3286
AhdI GACNNNNNGTC 2 cut(s) 552, 1276
AjnI CCWGG 3 cut(s) 385, 1638, 5306
AjuI GAANNNNNNNTTGG 4 cut(s) 839, 871, 1787, 1819
AleI CACNNNNGTG 1 cut(s) 4181
Alw21I GWGCWC 7 cut(s) 2976, 3769, 4309, 4353, 4408, 4690, 5726
AlwNI CAGNNNCTG 4 cut(s) 1421, 1517, 2362, 5563
Aor13HI TCCGGA 2 cut(s) 3042, 5372
AoxI GGCC 6 cut(s) 1387, 1866, 2337, 2908, 4698, 5815
ApaI GGGCCC 1 cut(s) 1870
ApoI RAATTY 9 cut(s) 1212, 2464, 2855, 3349, 3841, 4462, 4827, 5429, 5623
ArsI GACNNNNNNTTYG 6 cut(s) 30, 62, 1620, 1652, 4082, 4114
AseI ATTAAT 2 cut(s) 921, 4254
Asp700I GAANNNNTTC 6 cut(s) 2010, 2256, 4047, 5067, 5592, 5652
AspLEI GCGC 2 cut(s) 1730, 5389
AspS9I GGNCC 7 cut(s) 234, 476, 1636, 1866, 1867, 3130, 3493
AsuC2I CCSGG 1 cut(s) 5547
AsuHPI GGTGA 7 cut(s) 525, 2129, 3451, 3871, 4832, 5260, 5456
AsuII TTCGAA 1 cut(s) 1394
AvaII GGWCC 5 cut(s) 234, 476, 1636, 3130, 3493
AxyI CCTNAGG 1 cut(s) 4410
BaeGI GKGCMC 1 cut(s) 1870
BamHI GGATCC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 4541
BanII GRGCYC 6 cut(s) 785, 1870, 2976, 3769, 4309, 5726
BauI CACGAG 5 cut(s) 1494, 2052, 3371, 3588, 4689
BbrPI CACGTG 1 cut(s) 1707
BbsI GAAGAC 5 cut(s) 879, 909, 2558, 4020, 4278
Bbv12I GWGCWC 7 cut(s) 2976, 3769, 4309, 4353, 4408, 4690, 5726
BbvCI CCTCAGC 1 cut(s) 3273
BceAI ACGGC 2 cut(s) 3105, 4998
BciT130I CCWGG 3 cut(s) 387, 1640, 5308
BciVI GTATCC 3 cut(s) 220, 1948, 5800
BclI TGATCA 2 cut(s) 315, 5418
BcnI CCSGG 1 cut(s) 5547
BfoI RGCGCY 2 cut(s) 1731, 5390
BfrI CTTAAG 3 cut(s) 1252, 3500, 4655
BfuI GTATCC 3 cut(s) 220, 1948, 5800
BglII AGATCT 6 cut(s) 290, 760, 2147, 3424, 3802, 4901
BlpI GCTNAGC 3 cut(s) 3768, 3966, 5873
Bme1390I CCNGG 4 cut(s) 387, 1640, 5308, 5547
Bme18I GGWCC 5 cut(s) 234, 476, 1636, 3130, 3493
BmeRI GACNNNNNGTC 2 cut(s) 552, 1276
BmgT120I GGNCC 7 cut(s) 234, 476, 1636, 1866, 1867, 3130, 3493
BmiI GGNNCC 6 cut(s) 195, 477, 689, 976, 1868, 4543
BmrFI CCNGG 4 cut(s) 387, 1640, 5308, 5547
BmrI ACTGGG 2 cut(s) 2791, 4533
BmsI GCATC 8 cut(s) 328, 2193, 2659, 2973, 3178, 4837, 5556, 5951
BmuI ACTGGG 2 cut(s) 2791, 4533
BpiI GAAGAC 5 cut(s) 879, 909, 2558, 4020, 4278
BplI GAGNNNNNCTC 6 cut(s) 2192, 2224, 2372, 2404, 3467, 3499
BpmI CTGGAG 7 cut(s) 47, 777, 2342, 3542, 3548, 4934, 4955
Bpu10I CCTNAGC 3 cut(s) 1731, 3273, 5843
Bpu1102I GCTNAGC 3 cut(s) 3768, 3966, 5873
Bpu14I TTCGAA 1 cut(s) 1394
BpuMI CCSGG 1 cut(s) 5547
BsaAI YACGTR 2 cut(s) 1100, 1707
BsaBI GATNNNNATC 2 cut(s) 1440, 3641
BsaI GGTCTC 2 cut(s) 1943, 5352
BsaJI CCNNGG 4 cut(s) 2340, 4290, 4980, 5306
BsaWI WCCGGW 2 cut(s) 3042, 5372
Bsc4I CCNNNNNNNGG 7 cut(s) 89, 374, 2114, 2398, 3042, 3127, 5817
Bse118I RCCGGY 1 cut(s) 5817
Bse21I CCTNAGG 1 cut(s) 4410
Bse3DI GCAATG 4 cut(s) 868, 2415, 5388, 5684
Bse8I GATNNNNATC 2 cut(s) 1440, 3641
BseAI TCCGGA 2 cut(s) 3042, 5372
BseBI CCWGG 3 cut(s) 387, 1640, 5308
BseDI CCNNGG 4 cut(s) 2340, 4290, 4980, 5306
BseGI GGATG 6 cut(s) 914, 2100, 2318, 2493, 4369, 5493
BseJI GATNNNNATC 2 cut(s) 1440, 3641
BseLI CCNNNNNNNGG 7 cut(s) 89, 374, 2114, 2398, 3042, 3127, 5817
BseMI GCAATG 4 cut(s) 868, 2415, 5388, 5684
BseRI GAGGAG 3 cut(s) 1805, 2414, 3029
BseSI GKGCMC 1 cut(s) 1870
BseYI CCCAGC 1 cut(s) 822
BsgI GTGCAG 2 cut(s) 2675, 4594
Bsh1236I CGCG 1 cut(s) 4222
BshFI GGCC 6 cut(s) 1389, 1868, 2339, 2910, 4700, 5817
BshNI GGYRCC 1 cut(s) 4541
BsiHKAI GWGCWC 7 cut(s) 2976, 3769, 4309, 4353, 4408, 4690, 5726
BsiSI CCGG 4 cut(s) 3043, 5373, 5546, 5818
BslFI GGGAC 6 cut(s) 110, 489, 1194, 2812, 3207, 5289
BslI CCNNNNNNNGG 7 cut(s) 89, 374, 2114, 2398, 3042, 3127, 5817
BsmFI GGGAC 6 cut(s) 110, 489, 1194, 2812, 3207, 5289
BsmI GAATGC 7 cut(s) 1622, 2010, 2012, 2434, 3784, 4375, 4682
BsnI GGCC 6 cut(s) 1389, 1868, 2339, 2910, 4700, 5817
Bso31I GGTCTC 2 cut(s) 1943, 5352
Bsp119I TTCGAA 1 cut(s) 1394
Bsp120I GGGCCC 1 cut(s) 1866
Bsp1286I GDGCHC 9 cut(s) 785, 1870, 2976, 3769, 4309, 4353, 4408, 4690, 5726
Bsp13I TCCGGA 2 cut(s) 3042, 5372
Bsp1407I TGTACA 1 cut(s) 2998
Bsp1720I GCTNAGC 3 cut(s) 3768, 3966, 5873
Bsp19I CCATGG 1 cut(s) 4290
BspANI GGCC 6 cut(s) 1389, 1868, 2339, 2910, 4700, 5817
BspEI TCCGGA 2 cut(s) 3042, 5372
BspFNI CGCG 1 cut(s) 4222
BspHI TCATGA 2 cut(s) 1365, 2286
BspLI GGNNCC 6 cut(s) 195, 477, 689, 976, 1868, 4543
BspMAI CTGCAG 6 cut(s) 954, 1179, 1849, 3054, 4069, 4782
BspQI GCTCTTC 1 cut(s) 4341
BspT104I TTCGAA 1 cut(s) 1394
BspT107I GGYRCC 1 cut(s) 4541
BspTI CTTAAG 3 cut(s) 1252, 3500, 4655
BspTNI GGTCTC 2 cut(s) 1943, 5352
BsrDI GCAATG 4 cut(s) 868, 2415, 5388, 5684
BsrFI RCCGGY 1 cut(s) 5817
BsrGI TGTACA 1 cut(s) 2998
BssAI RCCGGY 1 cut(s) 5817
BssECI CCNNGG 4 cut(s) 2340, 4290, 4980, 5306
BssNAI GTATAC 1 cut(s) 299
BssSI CACGAG 5 cut(s) 1494, 2052, 3371, 3588, 4689
BssT1I CCWWGG 2 cut(s) 2340, 4290
Bst1107I GTATAC 1 cut(s) 299
Bst2BI CACGAG 5 cut(s) 1494, 2052, 3371, 3588, 4689
Bst2UI CCWGG 3 cut(s) 387, 1640, 5308
Bst6I CTCTTC 8 cut(s) 676, 1077, 2138, 2157, 3859, 4341, 4614, 5239
BstAFI CTTAAG 3 cut(s) 1252, 3500, 4655
BstAUI TGTACA 1 cut(s) 2998
BstBAI YACGTR 2 cut(s) 1100, 1707
BstBI TTCGAA 1 cut(s) 1394
BstC8I GCNNGC 7 cut(s) 785, 1870, 1974, 2592, 2646, 2658, 4353
BstDSI CCRYGG 2 cut(s) 4290, 4980
BstENI CCTNNNNNAGG 2 cut(s) 2112, 2396
BstF5I GGATG 6 cut(s) 914, 2100, 2318, 2493, 4369, 5493
BstFNI CGCG 1 cut(s) 4222
BstH2I RGCGCY 2 cut(s) 1731, 5390
BstHHI GCGC 2 cut(s) 1730, 5389
BstNI CCWGG 3 cut(s) 387, 1640, 5308
BstNSI RCATGY 4 cut(s) 404, 2415, 2571, 4636
BstSCI CCNGG 4 cut(s) 385, 1638, 5306, 5545
BstSLI GKGCMC 1 cut(s) 1870
BstSNI TACGTA 1 cut(s) 1100
BstUI CGCG 1 cut(s) 4222
BstV2I GAAGAC 5 cut(s) 879, 909, 2558, 4020, 4278
BstXI CCANNNNNNTGG 1 cut(s) 1603
BstZ17I GTATAC 1 cut(s) 299
Bsu36I CCTNAGG 1 cut(s) 4410
BsuI GTATCC 3 cut(s) 220, 1948, 5800
BsuRI GGCC 6 cut(s) 1389, 1868, 2339, 2910, 4700, 5817
BtgI CCRYGG 2 cut(s) 4290, 4980
BtsCI GGATG 6 cut(s) 914, 2100, 2318, 2493, 4369, 5493
BtsI GCAGTG 4 cut(s) 959, 3030, 4582, 5347
Cac8I GCNNGC 7 cut(s) 785, 1870, 1974, 2592, 2646, 2658, 4353
CaiI CAGNNNCTG 4 cut(s) 1421, 1517, 2362, 5563
CciI TCATGA 2 cut(s) 1365, 2286
CfoI GCGC 2 cut(s) 1730, 5389
Cfr10I RCCGGY 1 cut(s) 5817
Cfr13I GGNCC 7 cut(s) 234, 476, 1636, 1866, 1867, 3130, 3493
CseI GACGC 2 cut(s) 1742, 5564
CspCI CAANNNNNGTGG 2 cut(s) 2398, 2433
DraI TTTAAA 3 cut(s) 1306, 2112, 3354
DrdI GACNNNNNNGTC 1 cut(s) 5449
DriI GACNNNNNGTC 2 cut(s) 552, 1276
DseDI GACNNNNNNGTC 1 cut(s) 5449
Eam1104I CTCTTC 8 cut(s) 676, 1077, 2138, 2157, 3859, 4341, 4614, 5239
Eam1105I GACNNNNNGTC 2 cut(s) 552, 1276
EarI CTCTTC 8 cut(s) 676, 1077, 2138, 2157, 3859, 4341, 4614, 5239
EciI GGCGGA 2 cut(s) 1751, 4502
Ecl136II GAGCTC 4 cut(s) 2974, 3767, 4307, 5724
Eco105I TACGTA 1 cut(s) 1100
Eco130I CCWWGG 2 cut(s) 2340, 4290
Eco147I AGGCCT 1 cut(s) 1389
Eco24I GRGCYC 6 cut(s) 785, 1870, 2976, 3769, 4309, 5726
Eco31I GGTCTC 2 cut(s) 1943, 5352
Eco32I GATATC 2 cut(s) 3661, 4105
Eco47I GGWCC 5 cut(s) 234, 476, 1636, 3130, 3493
Eco53kI GAGCTC 4 cut(s) 2974, 3767, 4307, 5724
Eco57I CTGAAG 8 cut(s) 206, 1321, 3117, 4689, 5220, 5579, 5700, 5948
Eco72I CACGTG 1 cut(s) 1707
Eco81I CCTNAGG 1 cut(s) 4410
EcoICRI GAGCTC 4 cut(s) 2974, 3767, 4307, 5724
EcoNI CCTNNNNNAGG 2 cut(s) 2112, 2396
EcoO109I RGGNCCY 1 cut(s) 1866
EcoRII CCWGG 3 cut(s) 385, 1638, 5306
EcoRV GATATC 2 cut(s) 3661, 4105
EcoT14I CCWWGG 2 cut(s) 2340, 4290
EcoT22I ATGCAT 6 cut(s) 2012, 2417, 2573, 2988, 3171, 5856
EcoT38I GRGCYC 6 cut(s) 785, 1870, 2976, 3769, 4309, 5726
ErhI CCWWGG 2 cut(s) 2340, 4290
FaqI GGGAC 6 cut(s) 110, 489, 1194, 2812, 3207, 5289
FauI CCCGC 5 cut(s) 82, 130, 792, 1877, 4722
FauNDI CATATG 1 cut(s) 3774
FbaI TGATCA 2 cut(s) 315, 5418
FblI GTMKAC 2 cut(s) 298, 1159
FokI GGATG 6 cut(s) 901, 2107, 2325, 2480, 4376, 5500
FriOI GRGCYC 6 cut(s) 785, 1870, 2976, 3769, 4309, 5726
GlaI GCGC 2 cut(s) 1729, 5388
GsaI CCCAGC 1 cut(s) 826
GsuI CTGGAG 7 cut(s) 47, 777, 2342, 3542, 3548, 4934, 4955
HaeII RGCGCY 2 cut(s) 1731, 5390
HaeIII GGCC 6 cut(s) 1389, 1868, 2339, 2910, 4700, 5817
HapII CCGG 4 cut(s) 3043, 5373, 5546, 5818
HgaI GACGC 2 cut(s) 1742, 5564
HhaI GCGC 2 cut(s) 1730, 5389
Hin6I GCGC 2 cut(s) 1728, 5387
HinP1I GCGC 2 cut(s) 1728, 5387
HincII GTYRAC 6 cut(s) 420, 733, 1429, 1894, 2275, 4213
HindII GTYRAC 6 cut(s) 420, 733, 1429, 1894, 2275, 4213
HindIII AAGCTT 8 cut(s) 884, 1996, 2167, 2452, 3024, 3580, 4396, 5057
HpaI GTTAAC 1 cut(s) 420
HpaII CCGG 4 cut(s) 3043, 5373, 5546, 5818
HphI GGTGA 7 cut(s) 525, 2129, 3451, 3871, 4832, 5260, 5456
HspAI GCGC 2 cut(s) 1728, 5387
Kpn2I TCCGGA 2 cut(s) 3042, 5372
Ksp22I TGATCA 2 cut(s) 315, 5418
KspAI GTTAAC 1 cut(s) 420
LguI GCTCTTC 1 cut(s) 4341
LweI GCATC 8 cut(s) 328, 2193, 2659, 2973, 3178, 4837, 5556, 5951
MaeIII GTNAC 7 cut(s) 403, 1100, 1677, 1754, 3010, 3122, 4382
MfeI CAATTG 1 cut(s) 5223
MhlI GDGCHC 9 cut(s) 785, 1870, 2976, 3769, 4309, 4353, 4408, 4690, 5726
MmeI TCCRAC 4 cut(s) 2514, 2528, 3036, 5784
Mph1103I ATGCAT 6 cut(s) 2012, 2417, 2573, 2988, 3171, 5856
MroI TCCGGA 2 cut(s) 3042, 5372
MroXI GAANNNNTTC 6 cut(s) 2010, 2256, 4047, 5067, 5592, 5652
MslI CAYNNNNRTG 5 cut(s) 963, 2991, 3777, 4181, 5745
MspA1I CMGCKG 4 cut(s) 125, 415, 2320, 4337
MspCI CTTAAG 3 cut(s) 1252, 3500, 4655
MspI CCGG 4 cut(s) 3043, 5373, 5546, 5818
MspR9I CCNGG 4 cut(s) 387, 1640, 5308, 5547
MunI CAATTG 1 cut(s) 5223
Mva1269I GAATGC 7 cut(s) 1622, 2010, 2012, 2434, 3784, 4375, 4682
MvaI CCWGG 3 cut(s) 387, 1640, 5308
MvnI CGCG 1 cut(s) 4222
NciI CCSGG 1 cut(s) 5547
NcoI CCATGG 1 cut(s) 4290
NdeI CATATG 1 cut(s) 3774
NlaIV GGNNCC 6 cut(s) 195, 477, 689, 976, 1868, 4543
NmuCI GTSAC 4 cut(s) 403, 1754, 3010, 3122
NsiI ATGCAT 6 cut(s) 2012, 2417, 2573, 2988, 3171, 5856
NspI RCATGY 4 cut(s) 404, 2415, 2571, 4636
NspV TTCGAA 1 cut(s) 1394
OliI CACNNNNGTG 1 cut(s) 4181
PagI TCATGA 2 cut(s) 1365, 2286
PceI AGGCCT 1 cut(s) 1389
PciI ACATGT 1 cut(s) 400
PciSI GCTCTTC 1 cut(s) 4341
PctI GAATGC 7 cut(s) 1622, 2010, 2012, 2434, 3784, 4375, 4682
PdmI GAANNNNTTC 6 cut(s) 2010, 2256, 4047, 5067, 5592, 5652
PflFI GACNNNGTC 3 cut(s) 2935, 3198, 4804
PfoI TCCNGGA 2 cut(s) 385, 1638
PmaCI CACGTG 1 cut(s) 1707
PmlI CACGTG 1 cut(s) 1707
Ppu21I YACGTR 2 cut(s) 1100, 1707
PscI ACATGT 1 cut(s) 400
PshBI ATTAAT 2 cut(s) 921, 4254
PsiI TTATAA 1 cut(s) 261
Psp124BI GAGCTC 4 cut(s) 2976, 3769, 4309, 5726
Psp1406I AACGTT 5 cut(s) 1748, 3362, 3994, 5140, 5934
Psp6I CCWGG 3 cut(s) 385, 1638, 5306
PspCI CACGTG 1 cut(s) 1707
PspFI CCCAGC 1 cut(s) 822
PspGI CCWGG 3 cut(s) 385, 1638, 5306
PspN4I GGNNCC 6 cut(s) 195, 477, 689, 976, 1868, 4543
PspOMI GGGCCC 1 cut(s) 1866
PspPI GGNCC 7 cut(s) 234, 476, 1636, 1866, 1867, 3130, 3493
PstI CTGCAG 6 cut(s) 954, 1179, 1849, 3054, 4069, 4782
PstNI CAGNNNCTG 4 cut(s) 1421, 1517, 2362, 5563
PsyI GACNNNGTC 3 cut(s) 2935, 3198, 4804
PvuII CAGCTG 3 cut(s) 415, 2320, 4337
RseI CAYNNNNRTG 5 cut(s) 963, 2991, 3777, 4181, 5745
SacI GAGCTC 4 cut(s) 2976, 3769, 4309, 5726
SapI GCTCTTC 1 cut(s) 4341
Sau96I GGNCC 7 cut(s) 234, 476, 1636, 1866, 1867, 3130, 3493
ScrFI CCNGG 4 cut(s) 387, 1640, 5308, 5547
SduI GDGCHC 9 cut(s) 785, 1870, 2976, 3769, 4309, 4353, 4408, 4690, 5726
SfaNI GCATC 8 cut(s) 328, 2193, 2659, 2973, 3178, 4837, 5556, 5951
SfuI TTCGAA 1 cut(s) 1394
SinI GGWCC 5 cut(s) 234, 476, 1636, 3130, 3493
SmiMI CAYNNNNRTG 5 cut(s) 963, 2991, 3777, 4181, 5745
SnaBI TACGTA 1 cut(s) 1100
SseBI AGGCCT 1 cut(s) 1389
SspI AATATT 4 cut(s) 2349, 2476, 4926, 5161
SstI GAGCTC 4 cut(s) 2976, 3769, 4309, 5726
StuI AGGCCT 1 cut(s) 1389
StyD4I CCNGG 4 cut(s) 385, 1638, 5306, 5545
StyI CCWWGG 2 cut(s) 2340, 4290
TaqII GACCGA 1 cut(s) 3058
TatI WGTACW 3 cut(s) 2998, 4173, 5134
TseFI GTSAC 4 cut(s) 403, 1754, 3010, 3122
Tsp45I GTSAC 4 cut(s) 403, 1754, 3010, 3122
TspGWI ACGGA 4 cut(s) 121, 2756, 3485, 5500
Tth111I GACNNNGTC 3 cut(s) 2935, 3198, 4804
Vha464I CTTAAG 3 cut(s) 1252, 3500, 4655
VpaK11BI GGWCC 5 cut(s) 234, 476, 1636, 3130, 3493
VspI ATTAAT 2 cut(s) 921, 4254
XagI CCTNNNNNAGG 2 cut(s) 2112, 2396
XapI RAATTY 9 cut(s) 1212, 2464, 2855, 3349, 3841, 4462, 4827, 5429, 5623
XbaI TCTAGA 2 cut(s) 287, 2131
XceI RCATGY 4 cut(s) 404, 2415, 2571, 4636
XcmI CCANNNNNNNNNTGG 2 cut(s) 90, 3256
XmiI GTMKAC 2 cut(s) 298, 1159
XmnI GAANNNNTTC 6 cut(s) 2010, 2256, 4047, 5067, 5592, 5652
Zsp2I ATGCAT 6 cut(s) 2012, 2417, 2573, 2988, 3171, 5856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.