Rh7DG472500

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0003440
Physical Location & Seq
Reverse (-)
67597883 .. 67608136
10254 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG472500.1

Sequence Viewer

Length: 5454 bp
ATGTCGAGGATCACTAAGTGGAAGCTTGAGAAGACAAAAGTGAAGGTGGTTTTCAGGCTACAATTCAACGCTACACATATTCCCCAAAGCGGATGGGATAAGTTGTTTATATCTTTCATACCTGCTGATTCTGGAAAGGCAACTGCAAAGACAACCAAAGCAAATGTGAGGAATGGAACATGCAAATGGGCAGATCCTATCTACGAAACTACAAGACTTCTACAAGACACTAAAACAAAGAAATTTGATGAGAAGCTCTATAATCTTGTGGTGACCATGGGCTCTTCACGGTCTAGTGTCCTTGGGGAGACCAATATCAACCTTGCTGATTATGCCGACGCGTCAAAGCCTTCTGCTGTTGCACTGCCTCTACATGGATGTGATTCTGGAACTATTTTACATGTAACTGTACAGCTATTAACTTCCAAAACTGGTTTCAGAGAGTTTGAACAGCAGAGGGAGCTCAGAGAGAGCGGTCTATGTACAACGTCAGACCAAAGCAGAAATGATGTGTCCACTGCTAAAAGAATATCGTCTTCCGAAGATACAATCAATGCGAGAGTTAGGTTCAAAGAAGAACTCTCTCCCCTTGAAGACGAGGTTGGGCGGAATGAAGAGTTTCCAGACTCAACTGTAGGGTTTGATGGTTCTTCCAATACTTCAGAAAGTTTATACACTGAGAAACATGATACCTCCAGCACCCATGAGATTGACAGCCTTAAGAGTACAACCTCTGGTGATTTAGGTGGACTGTCCCTTGGTCAAAGTCCTCGAAAGGAGAAAGGAGACCACTCTGATCAACGTTTCTTGGCACAGGGGACCAGTGAGTGGGCTCATAGTTGGGCTTCTGACTATTCTGGAGATGCCGACTTGCCAAATGCTTATGAGGAGAATAGTAGACTTAGAGGAAGCTTGGAAGCAGCTGAGTCATCCATTCTTGAGCTTAAGCAGGAAGTAAGCTCTTTACAGAGCCAAGCTGATGAAATAGGAGTTGAAGCCCAAAAATTTTCCCTTCAACTTGATGCAGAAATTTGTTCAAGTGAACAGTTAGCCAAAGAAGTTTCTATACTGAGATCAGAATGTTCAAAATTGAAAGAAGATCTTGAAGTGCAGAAAAACTCCAAATTAAGAATTCCATTCACCAGCACAGAAACTATTGAGACAGGTCAGGAGGACTTTTTGCAGGATTTACAGCTCAGGTGGTTAAAGGGGCTTGGGGATGTGGAGGATAAAATTAAAGAGCTTCAAAGCAAAGCAACCGTTGGAGTACATGAGAGGGACTTCAGGTCCTTTCATTCAGATTTAGAGGCATTGCTTGGTGTTCTGCAAGTTCTAAAGCCAGTAACTGGACAACCGATTTTGGGGATGAACAAGGCAAGCATAAAGGAGACAAATGAAACGAGTGTACATAAAGATGAGCAATTAGTACTAGGAACTAGGTTTGATGCAGATTTCTATCCTGAAGGTATGCTTCATAGTCTAAGTATGCCTGGCTTGGTGTCACAAGAGTTTGAGTCTTTAGATGCCACAAATGCAATGAAAAGCAAATTCTTTGAGCTTTTAAGAGAGTTGGATGAATTAAAAGCTGAGCGAGAGAGCCTTGCCAAAAAAGCAGACCAGATGGAGTGTTACTATGAAGCTCTCATTCATGAATTAGAAGAAAATCAGAGACAGATGATGGGGGAGTTGCAGAGTCTCAGAAATGAGCATTCTACTTGCCTGTACACAATTTCATCTGCTAAAGCTGAGATGGGGAGAATCCAGCTAGATATGAACAATGAGATAACAAAATTTTCCAAAGAAAGGCATGATTTGGAATCTCTCACCAAGGAGCTCGAAAGAAGGGCTGCTACAGCAGAAGCAGCACTTAAAAGGGCACGCTTGAATTACTCCATTGCGGTGGACCATTTACAGAAGGACCTTGAATTGCTTTCTTCTCAGGTCCTGTCCATGCATGAGACTAATGAGAACCTCATTAAGCAGGCTTTTGCAGATTCTGTGCTTCCAAGCTTTCAGGGGCGTGAGGTGATGATGCAGAATCCGAAATGGGAATCAGAGATTTTTCAATCTGGAAAGCACATGCAGCGTCCAAACCAAAGCAATGGTGTAAAAAGACAACATTTAGACGGGGATATACTATCAGACGATCTGAGAAGATCTCTCCTCTTGCAGAAGGAAACATACCAAAAGGTTGAAGAAGAAGTTTATGAAGTGCATCTGGTGAATGTATATTTGGACATCTTCTCAAAGACCCTAGAGGTCACTTTGATTGAAGCAAGTGCTGACTTTGGATTGGTCAAAGAGAAAGTGCATGAACTTACACACCAGCTGGAGCTTTCAACTGAATCAAGGGAGTTATTGATGCTGAGGCTACAGACTGCCTTGGATGAGATTCGCTGCCTGAATGAAGACAAGTATATTTGGAATTCAAAGTGCAATGACTTGACTCTAAAGAACCAAAATTTGGAAGAAGATTTTCGGACTGCCATGGATGAGATTCGCTGCCTGAATGAGTACAAGGATACTTGCAATTCAAAGTGCAATGACTTGACTCTGAAGAACCACAGTTTGGAAGAAGATTTGCAGAAAGTTACTAGAGAAAATAATCTTCATTCTCAGAAGATTGCCGAATGGAAAGATCTTGTAAAAGAATATGAAACTTATGAGAGCAAATATAGAGCCTGCAGTACAGAAAAATTGGAGTTAGCAAATTTATTGGAAAAGGAAGCCCTAGAGAATAAGAATCTTCAAAACAGACTTTCATCTTTGCAGGAAGAGTTGAAAGCTGTCAGAAATGACTGTGATGAGCTGACATATGGAAAGGAGAGTCTACAGAATATTGTAAATTCTTCACAGGGTAAGTTGTGGAATCTGTTGGCATCATATGATATAAAGTATAAAGGTCTGTCTCTGTCTCTCTGCAGTGAATCTAACTCTCAAAATTTGGGATCCAAGGATTTAACTGCTGTGGTGGTGCAAATAGAAGAGCTTCAGCACAATTTATATGAGAAGATTGTCCAGCTTATGGAAGAGAAGAAAGACCTAGCACAAGAAAGAGATACTTTAAGAGCAGCCAATTCTGATAATCTGATCATGAAACAGAAATTTGAACAGGATCTACGGGGTATGATGGATAAACTAGATGTTTCAAATGCCCTGGTGCAAAAGCTTCAGTTACAAGTTGGGGCCATTGCTAACAAACTTCAGATTAGTTCTGAGATTGAAGAGAGATATGCACAACAGCACAAAGAACTGTTGGCCGATCTGGATCAGTTGGAAATGGAGCTGCAGCAAATTTCTTTTAAATACCAGGACCTTGCGGAGGAAATTATGGCGTTGGAGACTGTAACCGACGAACTAGGAAGGTGTAAACTGACGATAGCAGCATTATCGGAGGAAAAAGAAGCTCTAGTGGTGTCCTTGCAGGATAAAACTGAAGAATCTTTCAAGCTTTCTCTGGAGGTTAATAGTTTGCAAGGAAGTTTGCTGTCTTCACGTGATGAGTTGCACATTGAGAAAAATCACAGAGATAAGTTCGCCAGTACAATTTCAGATCTCACTTCCCAATTGGACCAGAAGCATAGTCAGTCTCTAGATTTTGATCAGCAGAAGGATGAGCTGGTCCACCTCAAACAGTTGTTATCAGAATCAGAGCTAGAGAAATCAAGAGTTTGTGGTCTCCTGTTGGATTCCGAGAAATGTCTAAAGGATGCTCGTGAAGAATGTTCTTCTATATCTGCTCTGCAAGCCCATTTGTCTGAAATGTATGAATTTTCAATAGCTGCTGATGTTGGACTCACTTTCACAAAGACTCAATACGAGACCAAGATTGAAGAGCTTGATCAGAAACTTCACTTTTCTGATAGCTGCCTATCTGAGCTTTGTGACAATCATCTTCATGTAGAAAATATGCTTAACAGATGTCTTGCAAGCGAAAAGCATTTAGTTGAAGAGAACACCAAACTAATGACAAGATTAAATGATGCTGGTGAAGAATGTGCTTTTGTATCTGCTCTGGAAGCTCAGCTGTCTGAAATGCATGAAGTTTCAATAGCTGCCGATGTTGGACTCACCTTCGTAGAAGCTCAGTATGGGGCCAAGATTGAAGAGCTTGGCCACAAACTTCACTCTTCTGATAGTCACCTTTCTGAGCTTTGCAACAATCACCTTCATGTGGAGAATAAGCTTAATGAATGTCTTGCTAGTGAAAGGCATTACATTGAAGAGAACACCAAATTAATGGCAAGTCTCAGCTCCCTTAACTCTGAGTTAGAAGCCTCCATTTTTCAAAACAAGATACTTCTTGATACAAACAGTTCCATGAGGACTGAACTCAAGGAATACAAGAAAAGAGCTGAAAATGCAGCAGCTATTGAGCAAGTGGATAAAAGTCAGTTTGCTCCTGAGATTGAAAGGCTGGAGCATATTGTGGTGACTTCTAATGAAGAGATTGATAACCTGATATTCTCCAAGGAGGAACTAGAAATCAAATATTTAGTGCTCAAAGCCAAGTTGGATGAACAGTGGACTCAAATAGCCTCTCTGGAAGCATATAAAGATGAATTGACAGTGATGCATAATAATGAATGCAATGATCTTAAACAGAGGCTTGCTGAACAGGTTTTGAAGGCAGAGGAATTTAAGAACTTGTCCATCCATTTCAAGGAGCTTAAAGACAAGTCTTGTGTTGAGTGTCTCCATGCACCTGACAAAAGAGAACCAGAAGCGCCGCCAGCTCCTATGCAAGAGTCCTTAAGAATCGCATTCATCAAAGAACAATATGAAACCAAGCTGCAAGAACTGAAACAACAACTTGCTATCTCCAAGAAGCATTGTGAGGAGATGCTGTGGAAATTACAAGATGCAATCAATGAAGTTGATAACAGGAAGAAATCTGAAGCTACTCATGTAAAGAGAAACGAAGAGCTAGGAATGAGGATCTTGGAATTGGAGTCTGAGCTGCAATCATTGCTTTCTGAAAAGCGTGAAATAATGAGAGCATATGATCTGATGAAGGCTGAAAAGGAATGTTCATTAATAAGCCTCGACTGCTGTAAAGAAGAAAAACAAAATCTTGAAGCATCTTTGCAGAAGTGCAACGAGGAAAAAGTTCAAATTACTTTAGAACTCACTTCAGCAAAAGATTTGCTTGAGAGCTCTTCATCTTCTAATCAGAGAGAAGGGAACGGAAGATTACACAAAGAAGATGGCATTTCTGATGAAGCAGCTGGACTGGAATACTTGAATTCCATTGATGAACCCGAGAAGGATGATATTGTATCCAGAGGTATAAATGGAATTTCTAGTGTTTTACCTTCGAAACAAATGGACGTGCTTAATAGTGATGTGAAGCATTTGGTTCTTGCCAATGAGCATTTCAGGGCCCAAAGCTTAAGGTCTAGCATGGAGAACTTAAATAAGGAGGTATGTATTTCTTTTGCAATGAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1817

Amino Acids

206.84

Weight (kDa)

4.99

Isoelectric Point (pI)

50.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 141 3.3e-25 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 130
AccB7I CCANNNNNTGG 5 cut(s) 828, 1346, 2464, 2569, 3025
AccBSI CCGCTC 1 cut(s) 474
AccI GTMKAC 2 cut(s) 898, 2830
AccII CGCG 1 cut(s) 341
AciI CCGC 6 cut(s) 90, 474, 607, 1898, 3320, 4730
AclI AACGTT 1 cut(s) 802
AclWI GGATC 7 cut(s) 17, 188, 2943, 2956, 3123, 3276, 4946
AcoI YGGCCR 2 cut(s) 3258, 4114
AcvI CACGTG 1 cut(s) 3497
AdeI CACNNNGTG 1 cut(s) 18
AflII CTTAAG 4 cut(s) 719, 944, 4753, 5392
AflIII ACRYGT 2 cut(s) 339, 400
AhdI GACNNNNNGTC 1 cut(s) 1285
AjiI CACGTC 1 cut(s) 5332
AjnI CCWGG 3 cut(s) 1489, 3156, 3309
Alw21I GWGCWC 4 cut(s) 465, 1836, 4503, 5159
AlwI GGATC 7 cut(s) 17, 188, 2943, 2956, 3123, 3276, 4946
AlwNI CAGNNNCTG 3 cut(s) 1346, 1945, 1997
Ama87I CYCGRG 1 cut(s) 5261
AoxI GGCC 5 cut(s) 3186, 3258, 4095, 4114, 5382
ApaI GGGCCC 1 cut(s) 5386
AseI ATTAAT 2 cut(s) 4238, 5036
Asp700I GAANNNNTTC 4 cut(s) 618, 2475, 2988, 5109
AspLEI GCGC 1 cut(s) 4729
AsuII TTCGAA 1 cut(s) 5318
AvaI CYCGRG 1 cut(s) 5261
AvaII GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3313, 3571, 3622
BaeGI GKGCMC 2 cut(s) 1879, 5386
BalI TGGCCA 1 cut(s) 4116
BamHI GGATCC 1 cut(s) 2948
BanII GRGCYC 6 cut(s) 284, 465, 835, 1836, 5159, 5386
BarI GAAGNNNNNNTAC 6 cut(s) 1050, 1082, 1834, 1866, 2165, 2197
BauI CACGAG 1 cut(s) 3714
BbrPI CACGTG 1 cut(s) 3497
BbsI GAAGAC 5 cut(s) 38, 528, 600, 2415, 3483
Bbv12I GWGCWC 4 cut(s) 465, 1836, 4503, 5159
BbvCI CCTCAGC 1 cut(s) 2366
BccI CCATC 8 cut(s) 87, 638, 1615, 1672, 1744, 3124, 4661, 5201
BcgI CGANNNNNNTGC 2 cut(s) 3372, 3406
BciT130I CCWGG 3 cut(s) 1491, 3158, 3311
BciVI GTATCC 2 cut(s) 2515, 5290
BclI TGATCA 4 cut(s) 796, 3090, 3601, 3841
BfmI CTRYAG 7 cut(s) 633, 1851, 2372, 2683, 2831, 2920, 3287
BfoI RGCGCY 1 cut(s) 4730
BfrI CTTAAG 4 cut(s) 719, 944, 4753, 5392
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 2 cut(s) 2515, 5290
BglII AGATCT 4 cut(s) 1099, 2156, 2638, 3553
BlpI GCTNAGC 2 cut(s) 1587, 4023
BmcAI AGTACT 1 cut(s) 1428
Bme1390I CCNGG 3 cut(s) 1491, 3158, 3311
Bme18I GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3313, 3571, 3622
BmeRI GACNNNNNGTC 1 cut(s) 1285
BmeT110I CYCGRG 1 cut(s) 5261
BmgBI CACGTC 1 cut(s) 5332
BmiI GGNNCC 5 cut(s) 820, 2950, 3187, 4096, 5384
BmrFI CCNGG 3 cut(s) 1491, 3158, 3311
BpiI GAAGAC 5 cut(s) 38, 528, 600, 2415, 3483
BplI GAGNNNNNCTC 2 cut(s) 2143, 2175
BpmI CTGGAG 5 cut(s) 679, 879, 2351, 3479, 4439
Bpu10I CCTNAGC 2 cut(s) 1196, 2366
Bpu1102I GCTNAGC 2 cut(s) 1587, 4023
Bpu14I TTCGAA 1 cut(s) 5318
BpuEI CTTGAG 4 cut(s) 47, 959, 4319, 5171
BsaAI YACGTR 1 cut(s) 3497
BsaBI GATNNNNATC 3 cut(s) 1455, 3267, 3600
BsaI GGTCTC 4 cut(s) 302, 780, 3683, 3815
BsaJI CCNNGG 9 cut(s) 276, 301, 757, 1827, 2382, 2487, 2952, 3156, 4470
BsaXI ACNNNNNCTCC 2 cut(s) 4832, 4862
Bse1I ACTGG 6 cut(s) 436, 822, 1340, 1351, 3540, 5238
Bse8I GATNNNNATC 3 cut(s) 1455, 3267, 3600
BseBI CCWGG 3 cut(s) 1491, 3158, 3311
BseDI CCNNGG 9 cut(s) 276, 301, 757, 1827, 2382, 2487, 2952, 3156, 4470
BseJI GATNNNNATC 3 cut(s) 1455, 3267, 3600
BseNI ACTGG 6 cut(s) 436, 822, 1340, 1351, 3540, 5238
BseRI GAGGAG 3 cut(s) 902, 2153, 4853
BseSI GKGCMC 2 cut(s) 1879, 5386
BsgI GTGCAG 1 cut(s) 1130
Bsh1236I CGCG 1 cut(s) 341
BshFI GGCC 5 cut(s) 3188, 3260, 4097, 4116, 5384
BsiHKAI GWGCWC 4 cut(s) 465, 1836, 4503, 5159
BsiHKCI CYCGRG 1 cut(s) 5261
BslFI GGGAC 3 cut(s) 739, 832, 1292
BsmFI GGGAC 3 cut(s) 739, 832, 1292
BsmI GAATGC 3 cut(s) 1708, 4592, 4763
BsnI GGCC 5 cut(s) 3188, 3260, 4097, 4116, 5384
Bso31I GGTCTC 4 cut(s) 302, 780, 3683, 3815
BsoBI CYCGRG 1 cut(s) 5261
Bsp119I TTCGAA 1 cut(s) 5318
Bsp120I GGGCCC 1 cut(s) 5382
Bsp1286I GDGCHC 8 cut(s) 284, 465, 835, 1836, 1879, 4503, 5159, 5386
Bsp1407I TGTACA 4 cut(s) 409, 482, 1405, 1722
Bsp1720I GCTNAGC 2 cut(s) 1587, 4023
Bsp19I CCATGG 2 cut(s) 276, 2487
BspACI CCGC 6 cut(s) 90, 474, 607, 1898, 3320, 4730
BspANI GGCC 5 cut(s) 3188, 3260, 4097, 4116, 5384
BspFNI CGCG 1 cut(s) 341
BspHI TCATGA 2 cut(s) 1648, 3093
BspLI GGNNCC 5 cut(s) 820, 2950, 3187, 4096, 5384
BspMAI CTGCAG 3 cut(s) 2687, 2924, 3291
BspMI ACCTGC 1 cut(s) 130
BspPI GGATC 7 cut(s) 17, 188, 2943, 2956, 3123, 3276, 4946
BspQI GCTCTTC 6 cut(s) 289, 2979, 3828, 4101, 4917, 5164
BspT104I TTCGAA 1 cut(s) 5318
BspTI CTTAAG 4 cut(s) 719, 944, 4753, 5392
BspTNI GGTCTC 4 cut(s) 302, 780, 3683, 3815
BsrBI CCGCTC 1 cut(s) 474
BsrGI TGTACA 4 cut(s) 409, 482, 1405, 1722
BsrI ACTGG 6 cut(s) 436, 822, 1340, 1351, 3540, 5238
BssECI CCNNGG 9 cut(s) 276, 301, 757, 1827, 2382, 2487, 2952, 3156, 4470
BssSI CACGAG 1 cut(s) 3714
BssT1I CCWWGG 8 cut(s) 276, 301, 757, 1827, 2382, 2487, 2952, 4470
Bst2BI CACGAG 1 cut(s) 3714
Bst2UI CCWGG 3 cut(s) 1491, 3158, 3311
BstAFI CTTAAG 4 cut(s) 719, 944, 4753, 5392
BstAPI GCANNNNNTGC 1 cut(s) 4969
BstAUI TGTACA 4 cut(s) 409, 482, 1405, 1722
BstBAI YACGTR 1 cut(s) 3497
BstBI TTCGAA 1 cut(s) 5318
BstC8I GCNNGC 8 cut(s) 1378, 1879, 1983, 2683, 3747, 3931, 4611, 4734
BstDSI CCRYGG 2 cut(s) 276, 2487
BstEII GGTNACC 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 3320
BstFNI CGCG 1 cut(s) 341
BstH2I RGCGCY 1 cut(s) 4730
BstHHI GCGC 1 cut(s) 4729
BstNI CCWGG 3 cut(s) 1491, 3158, 3311
BstNSI RCATGY 3 cut(s) 183, 404, 2083
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 3 cut(s) 1489, 3156, 3309
BstSFI CTRYAG 7 cut(s) 633, 1851, 2372, 2683, 2831, 2920, 3287
BstSLI GKGCMC 2 cut(s) 1879, 5386
BstUI CGCG 1 cut(s) 341
BstV2I GAAGAC 5 cut(s) 38, 528, 600, 2415, 3483
BstX2I RGATCY 8 cut(s) 193, 1099, 2156, 2638, 2948, 3115, 3553, 4938
BstXI CCANNNNNNTGG 3 cut(s) 1900, 2102, 4240
BstYI RGATCY 8 cut(s) 193, 1099, 2156, 2638, 2948, 3115, 3553, 4938
BsuI GTATCC 2 cut(s) 2515, 5290
BsuRI GGCC 5 cut(s) 3188, 3260, 4097, 4116, 5384
BtgI CCRYGG 2 cut(s) 276, 2487
BtrI CACGTC 1 cut(s) 5332
BtsI GCAGTG 3 cut(s) 362, 516, 2929
BtsIMutI CAGTG 7 cut(s) 362, 516, 675, 829, 2929, 4529, 4575
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 8 cut(s) 1378, 1879, 1983, 2683, 3747, 3931, 4611, 4734
CaiI CAGNNNCTG 3 cut(s) 1346, 1945, 1997
CciI TCATGA 2 cut(s) 1648, 3093
CfoI GCGC 1 cut(s) 4729
CseI GACGC 3 cut(s) 330, 347, 2075
DraI TTTAAA 1 cut(s) 3304
DraIII CACNNNGTG 1 cut(s) 18
DriI GACNNNNNGTC 1 cut(s) 1285
EaeI YGGCCR 2 cut(s) 3258, 4114
Eam1105I GACNNNNNGTC 1 cut(s) 1285
EciI GGCGGA 1 cut(s) 622
Ecl136II GAGCTC 3 cut(s) 463, 1834, 5157
Eco130I CCWWGG 8 cut(s) 276, 301, 757, 1827, 2382, 2487, 2952, 4470
Eco24I GRGCYC 6 cut(s) 284, 465, 835, 1836, 5159, 5386
Eco31I GGTCTC 4 cut(s) 302, 780, 3683, 3815
Eco47I GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3313, 3571, 3622
Eco53kI GAGCTC 3 cut(s) 463, 1834, 5157
Eco72I CACGTG 1 cut(s) 3497
Eco88I CYCGRG 1 cut(s) 5261
Eco91I GGTNACC 1 cut(s) 271
EcoICRI GAGCTC 3 cut(s) 463, 1834, 5157
EcoNI CCTNNNNNAGG 1 cut(s) 3320
EcoO109I RGGNCCY 5 cut(s) 1287, 1918, 1942, 3313, 5382
EcoO65I GGTNACC 1 cut(s) 271
EcoRI GAATTC 3 cut(s) 1131, 2425, 5245
EcoRII CCWGG 3 cut(s) 1489, 3156, 3309
EcoT14I CCWWGG 8 cut(s) 276, 301, 757, 1827, 2382, 2487, 2952, 4470
EcoT22I ATGCAT 3 cut(s) 1956, 4041, 4578
EcoT38I GRGCYC 6 cut(s) 284, 465, 835, 1836, 5159, 5386
ErhI CCWWGG 8 cut(s) 276, 301, 757, 1827, 2382, 2487, 2952, 4470
FaqI GGGAC 3 cut(s) 739, 832, 1292
FauNDI CATATG 3 cut(s) 2815, 2884, 5002
FbaI TGATCA 4 cut(s) 796, 3090, 3601, 3841
FblI GTMKAC 2 cut(s) 898, 2830
FriOI GRGCYC 6 cut(s) 284, 465, 835, 1836, 5159, 5386
GlaI GCGC 1 cut(s) 4728
GsuI CTGGAG 5 cut(s) 679, 879, 2351, 3479, 4439
HaeII RGCGCY 1 cut(s) 4730
HaeIII GGCC 5 cut(s) 3188, 3260, 4097, 4116, 5384
HgaI GACGC 3 cut(s) 330, 347, 2075
HhaI GCGC 1 cut(s) 4729
Hin6I GCGC 1 cut(s) 4727
HinP1I GCGC 1 cut(s) 4727
HindIII AAGCTT 7 cut(s) 23, 910, 2008, 3167, 3449, 4184, 5389
Hpy99I CGWCG 2 cut(s) 341, 3356
HpyCH4IV ACGT 4 cut(s) 488, 802, 3496, 5331
HpySE526I ACGT 4 cut(s) 488, 802, 3496, 5331
HspAI GCGC 1 cut(s) 4727
Ksp22I TGATCA 4 cut(s) 796, 3090, 3601, 3841
LguI GCTCTTC 6 cut(s) 289, 2979, 3828, 4101, 4917, 5164
LmnI GCTCC 9 cut(s) 460, 1831, 2332, 3283, 4259, 4405, 4420, 4666, 4741
MaeII ACGT 4 cut(s) 488, 802, 3496, 5331
MbiI CCGCTC 1 cut(s) 474
MfeI CAATTG 1 cut(s) 3566
MflI RGATCY 8 cut(s) 193, 1099, 2156, 2638, 2948, 3115, 3553, 4938
MhlI GDGCHC 8 cut(s) 284, 465, 835, 1836, 1879, 4503, 5159, 5386
MlsI TGGCCA 1 cut(s) 4116
MluI ACGCGT 1 cut(s) 339
MluNI TGGCCA 1 cut(s) 4116
MmeI TCCRAC 8 cut(s) 1243, 1551, 3255, 3318, 3666, 3772, 4045, 4494
Mox20I TGGCCA 1 cut(s) 4116
Mph1103I ATGCAT 3 cut(s) 1956, 4041, 4578
MroXI GAANNNNTTC 4 cut(s) 618, 2475, 2988, 5109
MscI TGGCCA 1 cut(s) 4116
MslI CAYNNNNRTG 7 cut(s) 184, 378, 1413, 1898, 3897, 4170, 4581
Msp20I TGGCCA 1 cut(s) 4116
MspA1I CMGCKG 4 cut(s) 923, 2329, 4027, 5228
MspCI CTTAAG 4 cut(s) 719, 944, 4753, 5392
MspR9I CCNGG 3 cut(s) 1491, 3158, 3311
MunI CAATTG 1 cut(s) 3566
Mva1269I GAATGC 3 cut(s) 1708, 4592, 4763
MvaI CCWGG 3 cut(s) 1491, 3158, 3311
MvnI CGCG 1 cut(s) 341
NcoI CCATGG 2 cut(s) 276, 2487
NdeI CATATG 3 cut(s) 2815, 2884, 5002
NlaIV GGNNCC 5 cut(s) 820, 2950, 3187, 4096, 5384
NmuCI GTSAC 6 cut(s) 271, 1500, 2260, 3884, 4139, 4432
NsiI ATGCAT 3 cut(s) 1956, 4041, 4578
NspI RCATGY 3 cut(s) 183, 404, 2083
NspV TTCGAA 1 cut(s) 5318
PagI TCATGA 2 cut(s) 1648, 3093
PciI ACATGT 1 cut(s) 400
PciSI GCTCTTC 6 cut(s) 289, 2979, 3828, 4101, 4917, 5164
PctI GAATGC 3 cut(s) 1708, 4592, 4763
PdmI GAANNNNTTC 4 cut(s) 618, 2475, 2988, 5109
PflMI CCANNNNNTGG 5 cut(s) 828, 1346, 2464, 2569, 3025
PmaCI CACGTG 1 cut(s) 3497
PmlI CACGTG 1 cut(s) 3497
Ppu21I YACGTR 1 cut(s) 3497
PpuMI RGGWCCY 4 cut(s) 1287, 1918, 1942, 3313
PscI ACATGT 1 cut(s) 400
PshBI ATTAAT 2 cut(s) 4238, 5036
Psp124BI GAGCTC 3 cut(s) 465, 1836, 5159
Psp1406I AACGTT 1 cut(s) 802
Psp5II RGGWCCY 4 cut(s) 1287, 1918, 1942, 3313
Psp6I CCWGG 3 cut(s) 1489, 3156, 3309
PspCI CACGTG 1 cut(s) 3497
PspEI GGTNACC 1 cut(s) 271
PspGI CCWGG 3 cut(s) 1489, 3156, 3309
PspN4I GGNNCC 5 cut(s) 820, 2950, 3187, 4096, 5384
PspOMI GGGCCC 1 cut(s) 5382
PspPPI RGGWCCY 4 cut(s) 1287, 1918, 1942, 3313
PsrI GAACNNNNNNTAC 2 cut(s) 3102, 3134
PstI CTGCAG 3 cut(s) 2687, 2924, 3291
PstNI CAGNNNCTG 3 cut(s) 1346, 1945, 1997
PsuI RGATCY 8 cut(s) 193, 1099, 2156, 2638, 2948, 3115, 3553, 4938
PvuII CAGCTG 4 cut(s) 923, 2329, 4027, 5228
RseI CAYNNNNRTG 7 cut(s) 184, 378, 1413, 1898, 3897, 4170, 4581
SacI GAGCTC 3 cut(s) 465, 1836, 5159
SapI GCTCTTC 6 cut(s) 289, 2979, 3828, 4101, 4917, 5164
ScaI AGTACT 1 cut(s) 1428
ScrFI CCNGG 3 cut(s) 1491, 3158, 3311
SduI GDGCHC 8 cut(s) 284, 465, 835, 1836, 1879, 4503, 5159, 5386
SfcI CTRYAG 7 cut(s) 633, 1851, 2372, 2683, 2831, 2920, 3287
SfuI TTCGAA 1 cut(s) 5318
SinI GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3313, 3571, 3622
SmiMI CAYNNNNRTG 7 cut(s) 184, 378, 1413, 1898, 3897, 4170, 4581
SmlI CTYRAG 8 cut(s) 26, 719, 938, 944, 4334, 4753, 5150, 5392
SmoI CTYRAG 8 cut(s) 26, 719, 938, 944, 4334, 4753, 5150, 5392
SsiI CCGC 6 cut(s) 90, 474, 607, 1898, 3320, 4730
SspI AATATT 2 cut(s) 2839, 4493
SstI GAGCTC 3 cut(s) 465, 1836, 5159
StyD4I CCNGG 3 cut(s) 1489, 3156, 3309
StyI CCWWGG 8 cut(s) 276, 301, 757, 1827, 2382, 2487, 2952, 4470
TaiI ACGT 4 cut(s) 491, 805, 3499, 5334
TaqI TCGA 5 cut(s) 5, 772, 1836, 5046, 5318
TauI GCSGC 1 cut(s) 4732
TscAI CASTG 7 cut(s) 369, 523, 682, 829, 2929, 4529, 4575
TseFI GTSAC 6 cut(s) 271, 1500, 2260, 3884, 4139, 4432
Tsp45I GTSAC 6 cut(s) 271, 1500, 2260, 3884, 4139, 4432
TspGWI ACGGA 1 cut(s) 5202
TspRI CASTG 7 cut(s) 369, 523, 682, 829, 2929, 4529, 4575
Van91I CCANNNNNTGG 5 cut(s) 828, 1346, 2464, 2569, 3025
Vha464I CTTAAG 4 cut(s) 719, 944, 4753, 5392
VpaK11BI GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3313, 3571, 3622
VspI ATTAAT 2 cut(s) 4238, 5036
XagI CCTNNNNNAGG 1 cut(s) 3320
XbaI TCTAGA 1 cut(s) 3592
XceI RCATGY 3 cut(s) 183, 404, 2083
XmiI GTMKAC 2 cut(s) 898, 2830
XmnI GAANNNNTTC 4 cut(s) 618, 2475, 2988, 5109
ZrmI AGTACT 1 cut(s) 1428
Zsp2I ATGCAT 3 cut(s) 1956, 4041, 4578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.