RLG00000000692

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003440
Physical Location & Seq
Forward (+)
3945177 .. 3953435
8259 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000692

Sequence Viewer

Length: 5919 bp
ATGTCGAGGATCACTAAGTGGAAGCTTGAGAAGACAAAAGTGAAGGTGGTTTTCAGGCTACAATTCAATGCTACACATATTCCCCAAAGTGGATGGGATAAGTTGTTTATATCTTTCATACCTGCTGATTCTGGAAAGGCAACTGCAAAGACAACCAAAGCGAATGTGAGGAATGGAACATGCAAATGGGCAGATCCTATCTACGAAACTACAAGACTTCTACAAGACACTAAAACAAAGAAATTTGATGAGAAGCTCTATAAACTTGTGGTGACCATGGGCTCTTCACGGTCTAGTGTCCTTGGGGAGACCAATATCAACCTTGCTGATTATGCCGACGCGTCAAAGCCTTCTGCTGTTGCACTGCCTCTACATGGATGTGATTCTGGAACTATTTTACATGTAACTGTACAGCTATTAACTTCCAAAACTGGTTTCAGAGAGTTTGAACAGCAGAGGGAGCTCAGAGAGAGCGGTCTATGTACAACATCAGACCAAAGCAGAAATGATGTGTCCACTGCTAAAAGAATATCGTCTTCCGATGATACAATCAATGCGAGAGTTAGGTTCAAAGAAGAACTCTCTCCCCTTGAAGACGAGGTTGGGCGGAATGAAGAGTTTCCAGACTCAACTGTAGGGTTTGATGGTTCTTCCAATACTTCAGAAAGTTTATACGCTGAGAAACATGATACCTCCAGCACCCATGAGATTGACAGCCTTAAGAGTACAACCTCTGGTGATTTAGGTGGACTGTCCCTTGGTCAAAGTCCTCGAAAGGAGAAAGGAGACCACTCTGATCAACGTTTCTTGGCACAGGGGACCAGTGAGTGGGCTCATAGCTGGGCTTCTGACTATTCTGGAGATGCCGACTTGCCAAATGCTTATGAGGAGAATAGTAGACTTAGAGGAAGCTTGGAAGCAGCTGAGTCATCCATTCTTGAGCTTAAGCAGGAAGTAAGCTCTTTACAGAGCCAAGCTGATGAAATAGGAGTCGAAGCCCAAAAATTTTCCCTTCAACTTGATGCAGAAATTTCTTCAAGTGAACGGTTAGCCAAAGAAGTTTCTATACTGAGATCAGAATGTTCAAAATTGAAAGAAGATCTTCAAGTGCAGAAAAACTCCAAATTAAGAATTCCATTCACCAGCACAGAAACTATTGAGACAGGTCAGGAGGACTTTTTGCAGGAGTTACAGCTCAGGTGGTTAAAGGGGCTTGGGGATGTGGAGGATAAAATTAAACAGCTTCAAAGCAAAGCAACCGTTGGAGTACATGAGAGGGACTTCAGGTCCTTTCATTCAGATTTAGAGGCATTGCTTGGTGTTCTGCAAGTTCTAAAGCCAGTAACTGAACAACCGATTTTGGGGATGAACAAGGCAAGCATAAAGGAGACAAATGAAATGAGTGTACATAAAGATGAGCAATTAGTACTAGGAACTAGGTCTGATGCAGATTTCTATCCCGAAGGTATGCTTCATGGTCTAAGCATGCCTGGCTTGGTGTCACAAGAGTTTGATTCTTTAGATGCTACAAATGCAATGAAAAGTAAATTCTTTGAGCTTTTAAGAGAGTTGGATGAATTAAAAGCTGAGCGAGAGAGCCTTGCCAAAAAAGCAGACCAGATGGAGTGCTACTATGAAGCTCTCATTCATGAATTAGAAGAAAATCAGAGACAGATGATGGGGGAGTTGCAGAGTCTCAGAAATGAGCATTCTACTTGCCTGTACACAATTTCATCTGCTAAAGCTGAGATGGGGAGAATCCAGCTAGATATGAACAATGAGATAACAAAATTTTCCAAAGAAAGGTATGATTTGGAATCTCTCACCAAGGAGCTCGAAAGAAGGGCTGCTACAGCAGAAGCAGCACTTAAAAGGGCACGCTTGAATTACTCCATTGCGGTGGACCATTTACAGAAGGACCTTGAATTGCTTTCTTCTCAGGTCCTGTCCATGCATGAGACTAATGAGAACCTCATTAAGCAGGCTTTTGCAGATTCTGTGCTTCCAAGCATTCAGGGGCGTGAGGTGATGATGCAGAATCCGAAATGGGAATCAGAGATTTTTCATTCTGGAAAGCACATGCAGCGTCCAAACCAAAGCAATGGTGTAAAAAGACAACATTTAGACGGGGATATACTATCAGACGATCTGAGAAGATCTCTCCTCTTGCAGAAGGAAACATACCAAAAGGTTGAAGAAGAAGTTTATGAAGTGCATCTGGTGAATGTATATTTGGACATCTTCTCAAAGACCCTAGAGGTCACTTTGATTGAAGCAAGTGCTGACTTTGGATTGGTCAAAGAGAAAGTGCATGAACTTACACACCAGCTGGAGCTTTCAACTGAATCAAAGGAGTTATTGATGCTGAGGCTACAGACTGCCTTGGATGAGATTCGCTGCCTGAATGAAGACAAGTATATTTGGAATTCAAAGTGCAATGACTTGACTCTAAAGAACCAAAATTTGGAAGAAGATTTTCGGACTGCCATGGATGAGTACAAGGATACTTGCAATTCAAAGTGCAATGACTTGACTCTGAAGAACCACAGTTTGGAAGAAGATTTGCAGAAAGTTACTAGAGAAAATAATCTTCATGCTCAGAAGATTGCTGAATGCAAAGATCTTGTAAAAGAATATGAAACCTATGAGAGCAAATATAGAGCCTGCAGTACAGAAAAATTGGAGTTAGCAAATTTATTGGAAAAGGAAGCCCTAGAGAATAAGAATCTTCAAAACAGACTTTCATCTTTGCAGGAAGAGTTGAAAGCTGTCAGAAATGACTGTGATGAGCTGACATATGGAAAGGAGAGTCTACAGAATATTGTAAATTCTTCACAGGGTAAGTTGTGGAATATGATGGCATCATATGATATAAAGTATAAAGTTCTGTCTCTGTCTCTCTGCAGTGAATATAACTATCAAAATTTGGAATCCAAGGATTTAACTGCTGTGGTGGTGCAAATAGAAGAGCTTCAGCACAATTTATATGAGAAGATTGTCCAGCTTATGGAAGAGAAGAAAGACCTAGCACAAGAAAGAGATACTTTAAGAGCAGCCAATTCTGATAATCTGATCATGAAACAGAAATTTGAACAGGATCTACGGGGTATGATGGATAAACTAGATGTTTCAAATGCCCTGGTGCAAAAGCTTCAGTTACAAGTTGGGGCCATTGCTAACAAACTTCAGATTAGCTCTGAAATTGAAGAGAGATATGCACAACAGCACAAAGAACTGCTGGCTGATCTGGATCAGTTGGAAATGGAGCTGCAGCAAATTTCTTTTAAATACCAGGACCTTGCGGAGGAAATTATGGCGTTGGAGACTGTAACCGACGAACTAGGAAGGTGTAAATTGACAATAGCAGCATTATCGGAGGAAAAAGAAGCTCTAGTGGTGTCCTTGCAGGATAAAACTGAAGAATCTTTCAAGCTTTCTCTGGAGGTTAATAGTTTGCAAGGAAGTTTGCTGTCTTCACGTGATGAGTTGCACATTGAGAAAAATCACAGAGATAAGTTAGCCAGTACAATTTCAGATCTCACTTCCCAATTGGACCAGAAGCATAGTCAGTCTCTAGATTTTGATCAGCAGAAGGATGAGCTGGTCCACCTCAAACAGTTGTTATCAGAATCAGAGCTAGAGAAATCAAGACTTTGTGGTCTCCTGTTGGATTCTGAGAAATGTCTAAAGGATGCTCGTGAAGAATGTTCTTCTATATCTGCTCTGGAAGCCCATTTGTCTGAAATGTATGAATTTTCAATAGCTGCTGATGTTGGACTCACTTTCACAAAGGCTCAATACGAGACCAAGATTGAAGAGCTTGATCAGAAACTTCACTTTTCTGATAGCTGCCTATCTGAGCTTTGCGACAATCATCTTCATGTAGAAAATATGCTTAACAGATGTCTTGCAAGCGAAAAGCATTTAGTTGAAGAGAACACCAAACTAATGACAAGATTAAATGATGCTGGTGAAGAATGTGCTTTTGTATCTGCTCTGGAAGCTCAGCTGTCTGAAGTGCATGAAGTTTCAATAGCTGCCGATGTTGGACTCACCTTCGTAGAAGCTCAGTATGGGGCCAAGATTGAAGAGCTTGGCCACAAACTTCACTCTTCTGATAGTCACCTTTCTGAGCTTTGCAACAATCACCTTCATGTGGAGAATAAGCTTAATGAATGTCTTGCTAGTGAAAGGCATTACATTGAAGAGAACACCAAATTAATGACAAGTCTCAGCTCCCTTAACTCTGAGTTAGAAGCCTCCATTTCTCAAAACAGGATACTTCTTGATACAAACAGTTCCATGAGGACTGAACTCGAGGAATACAAGAAAAGAGCTGAAAATGCAGCAGATATTGATCATGTGGATAAAAGTCAGTTTGCTCCTGAGATTGAAAGGCTGGAGCATATTGTGGTGACTTCTAAAGAAGAGATTGATAACCTGATATTCTCCAAGGAAGAACTAGAAATCAAATATTTAGTGCTCAAAGCCAAGTTGGATGAACAGTGCACTCAAATAGCCTCTCTGGAAGCATATAAAGATGAATTGACAGTGATGCATAATAATGAATGCAATGATCTTAAACAGAGGCTTGCTGAACAGGTTTTGAAGGCAGAGGAATTTAAGAACTTGTCCATCCATTTCAAGGAGCTTAAAGACAAGTCTTGTGTTGAGTGTCTCCATGCACCTGACAAAAGAGAACCAGAAGCGCCGCCAGCTCCTATGCAAGAGTCCTTAAGAATCGCATTCATCAAAGAACAATATGAAACCAAGCTGCAAGAACTGAAACAACAACTTGCTATCTCCAAGAAGCATTGTGAGGAGATGCTGTGGAAATTACAAGATGCAATCAATGAAGTTGATAACAGGAAGAAATCTGAAGCTACTCATGTAAAGAGAAATGAAGAGCTAGGAATGAGGATCTTGGAATTGGAGTCTGAGCTACAATCATTGCTTTCTGAAAAACGTGAAATAATGAGAGCATATGATCTGATGAAGGCTGAAAAGGAATGTTCATTAATAAGCCTCGACTGCTGTAAAGAAGAAAAACAAGATCTTGAAGCATCTTTGCAGAAGTGCAAAGAGGAAAAAGTTCAAATTACATTAGAACTCACTTCAGCAAAAGATTTGCTTGAGAGCTCTTCATCTTCTAATCAGAGAGAAGGGAATGGAAGATTACACAAAGAAGATGGCATTTCTGATGAAGCAGCTGGACTTGAATACTTGAATTCCATTGATGAACCCAAGAAGGATGATATTGTATCCAGAGGTATAAATGGAATTTCTAGTGTTTTACCTTCGAAACAAATGGATGTGCTTAATAGTGACGTGAAGCATTTGGTTCTGGCCAATGAGCATTTCAGGGCCCAAAGCTTAAGGTCTAGCATGGAGAACTTAAATAAGGAGTTGGAAAGGATGAAACATGAAAATTTGCAAGATGATCATCATTTTGACTCAAATTTTCCTGGTTTACAAAGAGACCTGATGCAATTAAATAAGGTTAATGAAGAATTGAGTAGTATTTTTCCCTTGTTCAACGAGTATTCTTGCAGTGGCAATGCATTAGAGAGAGTACTAGCTTTAGAAATTGAACTTGCAGAAGCATTGCAGGCAAAGAAGAAATCATCCGTCCAGTTTCAAAGTTCTTTCTTGAAACAACATGATGACGAGGAAGCAGTGTTTCACAGCTTTAGAGACATAAATGAGCTGATTAAAGACATGCTGGAGATCAAGGGAAGATATGCAACTGTGGAGACAGAACTTAAAGAAATGCATGACCGTTACTCTCAGTTAAGCCTTCAATTTGCAGAGGTTGAAGGAGAGAGGCAGAAACTAATGATGACTCTCAAGAATGTTCGAGCATCAAAGAAAGCACTATGCTTAAACCGCTCCTCGACAACCACTCTACTGGATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1973

Amino Acids

224.9

Weight (kDa)

5.03

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 141 2.5e-25 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3657
Acc36I ACCTGC 1 cut(s) 130
AccB7I CCANNNNNTGG 4 cut(s) 828, 2464, 2551, 3007
AccBSI CCGCTC 2 cut(s) 474, 5889
AccI GTMKAC 2 cut(s) 898, 2812
AccII CGCG 1 cut(s) 341
AciI CCGC 6 cut(s) 474, 607, 1898, 3302, 4712, 5887
AclI AACGTT 1 cut(s) 802
AclWI GGATC 6 cut(s) 17, 188, 3105, 3258, 4928, 5906
AcoI YGGCCR 2 cut(s) 4096, 5346
AcvI CACGTG 1 cut(s) 3479
AdeI CACNNNGTG 1 cut(s) 18
AflII CTTAAG 4 cut(s) 719, 944, 4735, 5374
AflIII ACRYGT 2 cut(s) 339, 400
AhdI GACNNNNNGTC 1 cut(s) 1285
AjiI CACGTC 1 cut(s) 5329
AjnI CCWGG 4 cut(s) 1489, 3138, 3291, 5464
Alw21I GWGCWC 5 cut(s) 465, 1836, 4485, 4511, 5141
Alw44I GTGCAC 1 cut(s) 4507
AlwI GGATC 6 cut(s) 17, 188, 3105, 3258, 4928, 5906
AlwNI CAGNNNCTG 3 cut(s) 1346, 1945, 1997
Ama87I CYCGRG 1 cut(s) 4316
AoxI GGCC 5 cut(s) 3168, 4077, 4096, 5346, 5364
ApaI GGGCCC 1 cut(s) 5368
ApaLI GTGCAC 1 cut(s) 4507
AseI ATTAAT 2 cut(s) 4220, 5018
Asp700I GAANNNNTTC 5 cut(s) 618, 1101, 2475, 2970, 5091
AspLEI GCGC 1 cut(s) 4711
AsuII TTCGAA 1 cut(s) 5300
AvaI CYCGRG 1 cut(s) 4316
AvaII GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3295, 3553, 3604
BaeGI GKGCMC 3 cut(s) 1879, 4511, 5368
BalI TGGCCA 2 cut(s) 4098, 5348
BamHI GGATCC 1 cut(s) 5911
BanII GRGCYC 6 cut(s) 284, 465, 835, 1836, 5141, 5368
BarI GAAGNNNNNNTAC 6 cut(s) 1050, 1082, 1834, 1866, 2165, 2197
BauI CACGAG 1 cut(s) 3696
BbrPI CACGTG 1 cut(s) 3479
BbsI GAAGAC 5 cut(s) 38, 528, 600, 2415, 3465
Bbv12I GWGCWC 5 cut(s) 465, 1836, 4485, 4511, 5141
BbvCI CCTCAGC 1 cut(s) 2366
BccI CCATC 9 cut(s) 87, 638, 1615, 1672, 1744, 2851, 3106, 4643, 5183
BcgI CGANNNNNNTGC 2 cut(s) 3354, 3388
BciT130I CCWGG 4 cut(s) 1491, 3140, 3293, 5466
BciVI GTATCC 3 cut(s) 2497, 4272, 5272
BclI TGATCA 6 cut(s) 796, 3072, 3583, 3823, 4357, 5440
BfmI CTRYAG 7 cut(s) 633, 1851, 2372, 2665, 2813, 2902, 3269
BfoI RGCGCY 1 cut(s) 4712
BfrI CTTAAG 4 cut(s) 719, 944, 4735, 5374
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 3 cut(s) 2497, 4272, 5272
BglII AGATCT 5 cut(s) 1099, 2156, 2620, 3535, 5053
BlpI GCTNAGC 2 cut(s) 1587, 4005
BmcAI AGTACT 2 cut(s) 1428, 5574
Bme1390I CCNGG 4 cut(s) 1491, 3140, 3293, 5466
Bme18I GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3295, 3553, 3604
BmeRI GACNNNNNGTC 1 cut(s) 1285
BmeT110I CYCGRG 1 cut(s) 4316
BmgBI CACGTC 1 cut(s) 5329
BmiI GGNNCC 5 cut(s) 820, 3169, 4078, 5366, 5913
BmrFI CCNGG 4 cut(s) 1491, 3140, 3293, 5466
BpiI GAAGAC 5 cut(s) 38, 528, 600, 2415, 3465
BplI GAGNNNNNCTC 2 cut(s) 2143, 2175
BpmI CTGGAG 6 cut(s) 679, 879, 2351, 3461, 4421, 5744
Bpu10I CCTNAGC 2 cut(s) 1196, 2366
Bpu1102I GCTNAGC 2 cut(s) 1587, 4005
Bpu14I TTCGAA 1 cut(s) 5300
BpuEI CTTGAG 4 cut(s) 47, 959, 5153, 5831
BsaAI YACGTR 1 cut(s) 3479
BsaBI GATNNNNATC 5 cut(s) 1455, 3249, 3582, 4356, 5442
BsaI GGTCTC 5 cut(s) 302, 780, 3665, 3797, 5472
BsaJI CCNNGG 9 cut(s) 276, 301, 757, 1827, 2382, 2487, 2934, 3138, 4452
BsaXI ACNNNNNCTCC 2 cut(s) 4814, 4844
Bse1I ACTGG 6 cut(s) 436, 822, 1340, 3522, 5632, 5913
Bse8I GATNNNNATC 5 cut(s) 1455, 3249, 3582, 4356, 5442
BseBI CCWGG 4 cut(s) 1491, 3140, 3293, 5466
BseDI CCNNGG 9 cut(s) 276, 301, 757, 1827, 2382, 2487, 2934, 3138, 4452
BseJI GATNNNNATC 5 cut(s) 1455, 3249, 3582, 4356, 5442
BseNI ACTGG 6 cut(s) 436, 822, 1340, 3522, 5632, 5913
BseRI GAGGAG 4 cut(s) 902, 2153, 4835, 5881
BseSI GKGCMC 3 cut(s) 1879, 4511, 5368
BseYI CCCAGC 1 cut(s) 840
BsgI GTGCAG 1 cut(s) 1130
Bsh1236I CGCG 1 cut(s) 341
BshFI GGCC 5 cut(s) 3170, 4079, 4098, 5348, 5366
BsiHKAI GWGCWC 5 cut(s) 465, 1836, 4485, 4511, 5141
BsiHKCI CYCGRG 1 cut(s) 4316
BslFI GGGAC 3 cut(s) 739, 832, 1292
BsmFI GGGAC 3 cut(s) 739, 832, 1292
BsmI GAATGC 5 cut(s) 1708, 2010, 2618, 4574, 4745
BsnI GGCC 5 cut(s) 3170, 4079, 4098, 5348, 5366
Bso31I GGTCTC 5 cut(s) 302, 780, 3665, 3797, 5472
BsoBI CYCGRG 1 cut(s) 4316
Bsp119I TTCGAA 1 cut(s) 5300
Bsp120I GGGCCC 1 cut(s) 5364
Bsp1286I GDGCHC 9 cut(s) 284, 465, 835, 1836, 1879, 4485, 4511, 5141, 5368
Bsp1407I TGTACA 4 cut(s) 409, 482, 1405, 1722
Bsp1720I GCTNAGC 2 cut(s) 1587, 4005
Bsp19I CCATGG 2 cut(s) 276, 2487
BspACI CCGC 6 cut(s) 474, 607, 1898, 3302, 4712, 5887
BspANI GGCC 5 cut(s) 3170, 4079, 4098, 5348, 5366
BspFNI CGCG 1 cut(s) 341
BspHI TCATGA 2 cut(s) 1648, 3075
BspLI GGNNCC 5 cut(s) 820, 3169, 4078, 5366, 5913
BspMAI CTGCAG 3 cut(s) 2669, 2906, 3273
BspMI ACCTGC 1 cut(s) 130
BspPI GGATC 6 cut(s) 17, 188, 3105, 3258, 4928, 5906
BspQI GCTCTTC 6 cut(s) 289, 2961, 3810, 4083, 4899, 5146
BspT104I TTCGAA 1 cut(s) 5300
BspTI CTTAAG 4 cut(s) 719, 944, 4735, 5374
BspTNI GGTCTC 5 cut(s) 302, 780, 3665, 3797, 5472
BsrBI CCGCTC 2 cut(s) 474, 5889
BsrGI TGTACA 4 cut(s) 409, 482, 1405, 1722
BsrI ACTGG 6 cut(s) 436, 822, 1340, 3522, 5632, 5913
BssECI CCNNGG 9 cut(s) 276, 301, 757, 1827, 2382, 2487, 2934, 3138, 4452
BssSI CACGAG 1 cut(s) 3696
BssT1I CCWWGG 8 cut(s) 276, 301, 757, 1827, 2382, 2487, 2934, 4452
Bst2BI CACGAG 1 cut(s) 3696
Bst2UI CCWGG 4 cut(s) 1491, 3140, 3293, 5466
BstAFI CTTAAG 4 cut(s) 719, 944, 4735, 5374
BstAUI TGTACA 4 cut(s) 409, 482, 1405, 1722
BstBAI YACGTR 1 cut(s) 3479
BstBI TTCGAA 1 cut(s) 5300
BstDSI CCRYGG 2 cut(s) 276, 2487
BstEII GGTNACC 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 3302
BstFNI CGCG 1 cut(s) 341
BstH2I RGCGCY 1 cut(s) 4712
BstHHI GCGC 1 cut(s) 4711
BstNI CCWGG 4 cut(s) 1491, 3140, 3293, 5466
BstNSI RCATGY 5 cut(s) 183, 404, 1489, 2083, 5722
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 4 cut(s) 1489, 3138, 3291, 5464
BstSFI CTRYAG 7 cut(s) 633, 1851, 2372, 2665, 2813, 2902, 3269
BstSLI GKGCMC 3 cut(s) 1879, 4511, 5368
BstUI CGCG 1 cut(s) 341
BstV2I GAAGAC 5 cut(s) 38, 528, 600, 2415, 3465
BstX2I RGATCY 9 cut(s) 193, 1099, 2156, 2620, 3097, 3535, 4920, 5053, 5911
BstXI CCANNNNNNTGG 3 cut(s) 1900, 2102, 5908
BstYI RGATCY 9 cut(s) 193, 1099, 2156, 2620, 3097, 3535, 4920, 5053, 5911
BsuI GTATCC 3 cut(s) 2497, 4272, 5272
BsuRI GGCC 5 cut(s) 3170, 4079, 4098, 5348, 5366
BtgI CCRYGG 2 cut(s) 276, 2487
BtrI CACGTC 1 cut(s) 5329
BtsI GCAGTG 5 cut(s) 362, 516, 2911, 5557, 5682
BtsIMutI CAGTG 8 cut(s) 362, 516, 829, 2911, 4511, 4557, 5557, 5682
BveI ACCTGC 1 cut(s) 130
CaiI CAGNNNCTG 3 cut(s) 1346, 1945, 1997
CciI TCATGA 2 cut(s) 1648, 3075
CfoI GCGC 1 cut(s) 4711
CseI GACGC 3 cut(s) 330, 347, 2075
DraI TTTAAA 1 cut(s) 3286
DraIII CACNNNGTG 1 cut(s) 18
DrdI GACNNNNNNGTC 1 cut(s) 3657
DriI GACNNNNNGTC 1 cut(s) 1285
DseDI GACNNNNNNGTC 1 cut(s) 3657
EaeI YGGCCR 2 cut(s) 4096, 5346
Eam1105I GACNNNNNGTC 1 cut(s) 1285
EciI GGCGGA 1 cut(s) 622
Ecl136II GAGCTC 3 cut(s) 463, 1834, 5139
Eco130I CCWWGG 8 cut(s) 276, 301, 757, 1827, 2382, 2487, 2934, 4452
Eco24I GRGCYC 6 cut(s) 284, 465, 835, 1836, 5141, 5368
Eco31I GGTCTC 5 cut(s) 302, 780, 3665, 3797, 5472
Eco47I GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3295, 3553, 3604
Eco53kI GAGCTC 3 cut(s) 463, 1834, 5139
Eco72I CACGTG 1 cut(s) 3479
Eco88I CYCGRG 1 cut(s) 4316
Eco91I GGTNACC 1 cut(s) 271
EcoICRI GAGCTC 3 cut(s) 463, 1834, 5139
EcoNI CCTNNNNNAGG 1 cut(s) 3302
EcoO109I RGGNCCY 5 cut(s) 1287, 1918, 1942, 3295, 5364
EcoO65I GGTNACC 1 cut(s) 271
EcoRI GAATTC 3 cut(s) 1131, 2425, 5227
EcoRII CCWGG 4 cut(s) 1489, 3138, 3291, 5464
EcoT14I CCWWGG 8 cut(s) 276, 301, 757, 1827, 2382, 2487, 2934, 4452
EcoT22I ATGCAT 4 cut(s) 1956, 4560, 5563, 5775
EcoT38I GRGCYC 6 cut(s) 284, 465, 835, 1836, 5141, 5368
ErhI CCWWGG 8 cut(s) 276, 301, 757, 1827, 2382, 2487, 2934, 4452
FaqI GGGAC 3 cut(s) 739, 832, 1292
FauNDI CATATG 3 cut(s) 2797, 2866, 4984
FbaI TGATCA 6 cut(s) 796, 3072, 3583, 3823, 4357, 5440
FblI GTMKAC 2 cut(s) 898, 2812
FriOI GRGCYC 6 cut(s) 284, 465, 835, 1836, 5141, 5368
GlaI GCGC 1 cut(s) 4710
GsaI CCCAGC 1 cut(s) 844
GsuI CTGGAG 6 cut(s) 679, 879, 2351, 3461, 4421, 5744
HaeII RGCGCY 1 cut(s) 4712
HaeIII GGCC 5 cut(s) 3170, 4079, 4098, 5348, 5366
HgaI GACGC 3 cut(s) 330, 347, 2075
HhaI GCGC 1 cut(s) 4711
Hin6I GCGC 1 cut(s) 4709
HinP1I GCGC 1 cut(s) 4709
HindIII AAGCTT 6 cut(s) 23, 910, 3149, 3431, 4166, 5371
Hpy99I CGWCG 2 cut(s) 341, 3338
HpyCH4IV ACGT 4 cut(s) 802, 3478, 4966, 5328
HpySE526I ACGT 4 cut(s) 802, 3478, 4966, 5328
HspAI GCGC 1 cut(s) 4709
Ksp22I TGATCA 6 cut(s) 796, 3072, 3583, 3823, 4357, 5440
LguI GCTCTTC 6 cut(s) 289, 2961, 3810, 4083, 4899, 5146
MaeII ACGT 4 cut(s) 802, 3478, 4966, 5328
MbiI CCGCTC 2 cut(s) 474, 5889
MfeI CAATTG 1 cut(s) 3548
MflI RGATCY 9 cut(s) 193, 1099, 2156, 2620, 3097, 3535, 4920, 5053, 5911
MhlI GDGCHC 9 cut(s) 284, 465, 835, 1836, 1879, 4485, 4511, 5141, 5368
MlsI TGGCCA 2 cut(s) 4098, 5348
MluI ACGCGT 1 cut(s) 339
MluNI TGGCCA 2 cut(s) 4098, 5348
MmeI TCCRAC 9 cut(s) 1243, 1551, 3237, 3300, 3648, 3754, 4027, 4476, 5388
Mox20I TGGCCA 2 cut(s) 4098, 5348
Mph1103I ATGCAT 4 cut(s) 1956, 4560, 5563, 5775
MroXI GAANNNNTTC 5 cut(s) 618, 1101, 2475, 2970, 5091
MscI TGGCCA 2 cut(s) 4098, 5348
MslI CAYNNNNRTG 7 cut(s) 184, 378, 1413, 1898, 3879, 4152, 4563
Msp20I TGGCCA 2 cut(s) 4098, 5348
MspA1I CMGCKG 4 cut(s) 923, 2329, 4009, 5210
MspCI CTTAAG 4 cut(s) 719, 944, 4735, 5374
MspR9I CCNGG 4 cut(s) 1491, 3140, 3293, 5466
MunI CAATTG 1 cut(s) 3548
Mva1269I GAATGC 5 cut(s) 1708, 2010, 2618, 4574, 4745
MvaI CCWGG 4 cut(s) 1491, 3140, 3293, 5466
MvnI CGCG 1 cut(s) 341
NcoI CCATGG 2 cut(s) 276, 2487
NdeI CATATG 3 cut(s) 2797, 2866, 4984
NlaIV GGNNCC 5 cut(s) 820, 3169, 4078, 5366, 5913
NmuCI GTSAC 6 cut(s) 271, 1500, 2260, 4121, 4414, 5324
NsiI ATGCAT 4 cut(s) 1956, 4560, 5563, 5775
NspI RCATGY 5 cut(s) 183, 404, 1489, 2083, 5722
NspV TTCGAA 1 cut(s) 5300
PaeI GCATGC 1 cut(s) 1489
PaeR7I CTCGAG 1 cut(s) 4316
PagI TCATGA 2 cut(s) 1648, 3075
PciI ACATGT 1 cut(s) 400
PciSI GCTCTTC 6 cut(s) 289, 2961, 3810, 4083, 4899, 5146
PctI GAATGC 5 cut(s) 1708, 2010, 2618, 4574, 4745
PdmI GAANNNNTTC 5 cut(s) 618, 1101, 2475, 2970, 5091
PflMI CCANNNNNTGG 4 cut(s) 828, 2464, 2551, 3007
PmaCI CACGTG 1 cut(s) 3479
PmlI CACGTG 1 cut(s) 3479
Ppu21I YACGTR 1 cut(s) 3479
PpuMI RGGWCCY 4 cut(s) 1287, 1918, 1942, 3295
PscI ACATGT 1 cut(s) 400
PshBI ATTAAT 2 cut(s) 4220, 5018
Psp124BI GAGCTC 3 cut(s) 465, 1836, 5141
Psp1406I AACGTT 1 cut(s) 802
Psp5II RGGWCCY 4 cut(s) 1287, 1918, 1942, 3295
Psp6I CCWGG 4 cut(s) 1489, 3138, 3291, 5464
PspCI CACGTG 1 cut(s) 3479
PspEI GGTNACC 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 840
PspGI CCWGG 4 cut(s) 1489, 3138, 3291, 5464
PspN4I GGNNCC 5 cut(s) 820, 3169, 4078, 5366, 5913
PspOMI GGGCCC 1 cut(s) 5364
PspPPI RGGWCCY 4 cut(s) 1287, 1918, 1942, 3295
PspXI VCTCGAGB 1 cut(s) 4316
PsrI GAACNNNNNNTAC 2 cut(s) 3084, 3116
PstI CTGCAG 3 cut(s) 2669, 2906, 3273
PstNI CAGNNNCTG 3 cut(s) 1346, 1945, 1997
PsuI RGATCY 9 cut(s) 193, 1099, 2156, 2620, 3097, 3535, 4920, 5053, 5911
PvuII CAGCTG 4 cut(s) 923, 2329, 4009, 5210
RseI CAYNNNNRTG 7 cut(s) 184, 378, 1413, 1898, 3879, 4152, 4563
SacI GAGCTC 3 cut(s) 465, 1836, 5141
SapI GCTCTTC 6 cut(s) 289, 2961, 3810, 4083, 4899, 5146
ScaI AGTACT 2 cut(s) 1428, 5574
ScrFI CCNGG 4 cut(s) 1491, 3140, 3293, 5466
SduI GDGCHC 9 cut(s) 284, 465, 835, 1836, 1879, 4485, 4511, 5141, 5368
SfcI CTRYAG 7 cut(s) 633, 1851, 2372, 2665, 2813, 2902, 3269
Sfr274I CTCGAG 1 cut(s) 4316
SfuI TTCGAA 1 cut(s) 5300
SinI GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3295, 3553, 3604
SlaI CTCGAG 1 cut(s) 4316
SmiMI CAYNNNNRTG 7 cut(s) 184, 378, 1413, 1898, 3879, 4152, 4563
SmlI CTYRAG 9 cut(s) 26, 719, 938, 944, 4316, 4735, 5132, 5374, 5846
SmoI CTYRAG 9 cut(s) 26, 719, 938, 944, 4316, 4735, 5132, 5374, 5846
SphI GCATGC 1 cut(s) 1489
SsiI CCGC 6 cut(s) 474, 607, 1898, 3302, 4712, 5887
SspI AATATT 2 cut(s) 2821, 4475
SstI GAGCTC 3 cut(s) 465, 1836, 5141
StyD4I CCNGG 4 cut(s) 1489, 3138, 3291, 5464
StyI CCWWGG 8 cut(s) 276, 301, 757, 1827, 2382, 2487, 2934, 4452
TaiI ACGT 4 cut(s) 805, 3481, 4969, 5331
TaqI TCGA 9 cut(s) 5, 772, 993, 1836, 4317, 5028, 5300, 5857, 5894
TauI GCSGC 1 cut(s) 4714
TscAI CASTG 8 cut(s) 369, 523, 829, 2911, 4511, 4557, 5557, 5682
TseFI GTSAC 6 cut(s) 271, 1500, 2260, 4121, 4414, 5324
Tsp45I GTSAC 6 cut(s) 271, 1500, 2260, 4121, 4414, 5324
TspGWI ACGGA 1 cut(s) 5617
TspRI CASTG 8 cut(s) 369, 523, 829, 2911, 4511, 4557, 5557, 5682
Van91I CCANNNNNTGG 4 cut(s) 828, 2464, 2551, 3007
Vha464I CTTAAG 4 cut(s) 719, 944, 4735, 5374
VneI GTGCAC 1 cut(s) 4507
VpaK11BI GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3295, 3553, 3604
VspI ATTAAT 2 cut(s) 4220, 5018
XagI CCTNNNNNAGG 1 cut(s) 3302
XbaI TCTAGA 1 cut(s) 3574
XceI RCATGY 5 cut(s) 183, 404, 1489, 2083, 5722
XhoI CTCGAG 1 cut(s) 4316
XmiI GTMKAC 2 cut(s) 898, 2812
XmnI GAANNNNTTC 5 cut(s) 618, 1101, 2475, 2970, 5091
ZrmI AGTACT 2 cut(s) 1428, 5574
Zsp2I ATGCAT 4 cut(s) 1956, 4560, 5563, 5775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.