pycom08g15100

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003440
Physical Location & Seq
Forward (+)
14590131 .. 14590466
336 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g15100.1

Sequence Viewer

Length: 336 bp
ATGCATGAATTTTCAATAGCTGCTGATGTTGGATTCACTTTCACAAAGGCTCAATATGAGACAAGGATAGAAGAACTAGAAAGGTATAACTTGACAATAACAGCACTGTTAGAGGAAAAAGAAGTTTTGATGGCGTCTTTACAGGATAAAACTCAAGAATCTTCCAGGCTTGCTCTCGAGCTTAATAGTTTGCAAGGGAGTTTGGTTTCTTTGTATGATGAGGTGCAAACAGAAAGAAATCTAAAGGATAAGTTAGAGAGTACAATTACAGAGCTCACTTCCCATTTGAATGAGAAGCATTGCCAGTTGCTAGGTTTTGATCGAGCAGAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

112

Amino Acids

12.7

Weight (kDa)

4.66

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 8
AcyI GRCGYC 1 cut(s) 134
AfaI GTAC 1 cut(s) 262
AgsI TTSAA 2 cut(s) 15, 289
AjnI CCWGG 1 cut(s) 164
AluBI AGCT 3 cut(s) 20, 181, 274
AluI AGCT 3 cut(s) 20, 181, 274
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 176
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 1 cut(s) 8
AvaI CYCGRG 1 cut(s) 176
BanII GRGCYC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 7
BccI CCATC 1 cut(s) 124
BciT130I CCWGG 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 2 cut(s) 77, 311
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
Bme1390I CCNGG 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 176
BmrFI CCNGG 1 cut(s) 166
BpuEI CTTGAG 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 134
Bse1I ACTGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 298
BseBI CCWGG 1 cut(s) 166
BseMI GCAATG 1 cut(s) 298
BseNI ACTGG 1 cut(s) 304
BseXI GCAGC 1 cut(s) 7
BsiHKAI GWGCWC 1 cut(s) 276
BsiHKCI CYCGRG 1 cut(s) 176
BsmAI GTCTC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 298
BsrI ACTGG 1 cut(s) 304
BssMI GATC 1 cut(s) 319
BssNI GRCGYC 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 166
Bst4CI ACNGT 1 cut(s) 108
BstACI GRCGYC 1 cut(s) 134
BstC8I GCNNGC 1 cut(s) 171
BstKTI GATC 1 cut(s) 322
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 1 cut(s) 319
BstNI CCWGG 1 cut(s) 166
BstSCI CCNGG 1 cut(s) 164
BstV1I GCAGC 1 cut(s) 7
BtsIMutI CAGTG 1 cut(s) 104
Cac8I GCNNGC 1 cut(s) 171
CseI GACGC 1 cut(s) 123
Csp6I GTAC 1 cut(s) 261
CviAII CATG 1 cut(s) 5
CviJI RGCY 6 cut(s) 20, 50, 169, 181, 274, 333
CviKI_1 RGCY 6 cut(s) 20, 50, 169, 181, 274, 333
CviQI GTAC 1 cut(s) 261
DpnI GATC 1 cut(s) 321
DpnII GATC 1 cut(s) 319
Ecl136II GAGCTC 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 274
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 164
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 276
FaeI CATG 1 cut(s) 8
FaiI YATR 4 cut(s) 6, 57, 87, 216
FatI CATG 1 cut(s) 4
Fnu4HI GCNGC 1 cut(s) 21
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 2 cut(s) 77, 311
GluI GCNGC 1 cut(s) 21
HgaI GACGC 1 cut(s) 123
Hin1I GRCGYC 1 cut(s) 134
Hin1II CATG 1 cut(s) 8
HinfI GANTC 2 cut(s) 33, 158
Hpy188III TCNNGA 2 cut(s) 155, 176
HpyAV CCTTC 1 cut(s) 323
HpyCH4III ACNGT 1 cut(s) 108
HpyCH4V TGCA 3 cut(s) 4, 193, 226
Hsp92I GRCGYC 1 cut(s) 134
Hsp92II CATG 1 cut(s) 8
Kzo9I GATC 1 cut(s) 319
LpnPI CCDG 4 cut(s) 128, 151, 178, 317
Lsp1109I GCAGC 1 cut(s) 7
MaeI CTAG 2 cut(s) 77, 311
MalI GATC 1 cut(s) 321
MboI GATC 1 cut(s) 319
MboII GAAGA 2 cut(s) 83, 153
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 2 cut(s) 8, 264
MmeI TCCRAC 1 cut(s) 10
MnlI CCTC 2 cut(s) 106, 214
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 1 cut(s) 288
MspR9I CCNGG 1 cut(s) 166
MvaI CCWGG 1 cut(s) 166
NdeII GATC 1 cut(s) 319
NlaIII CATG 1 cut(s) 8
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 176
PfeI GAWTC 2 cut(s) 33, 158
PkrI GCNGC 1 cut(s) 22
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 164
PspGI CCWGG 1 cut(s) 164
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
RseI CAYNNNNRTG 1 cut(s) 288
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 1 cut(s) 319
ScrFI CCNGG 1 cut(s) 166
SduI GDGCHC 1 cut(s) 276
SetI ASST 6 cut(s) 22, 86, 183, 225, 276, 316
Sfr274I CTCGAG 1 cut(s) 176
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 288
SmlI CTYRAG 2 cut(s) 153, 176
SmoI CTYRAG 2 cut(s) 153, 176
Sse9I AATT 2 cut(s) 8, 264
SspMI CTAG 2 cut(s) 77, 311
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 1 cut(s) 164
TaaI ACNGT 1 cut(s) 108
TaqI TCGA 2 cut(s) 177, 322
TasI AATT 2 cut(s) 8, 264
TatI WGTACW 1 cut(s) 260
TfiI GAWTC 2 cut(s) 33, 158
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 1 cut(s) 111
TseI GCWGC 1 cut(s) 20
TspDTI ATGAA 1 cut(s) 21
TspRI CASTG 1 cut(s) 111
XapI RAATTY 1 cut(s) 8
XhoI CTCGAG 1 cut(s) 176
XspI CTAG 2 cut(s) 77, 311
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.