MD08G1176200.v1.1

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003440
Physical Location & Seq
Forward (+)
21378944 .. 21390493
11550 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1176200.v1.1.491

Sequence Viewer

Length: 6603 bp
ATGTCGAGGATCACTAGGTGGAAGCTTGAGAAGACAAAAGTAAAAGTGGTTTTCAGGTTACAGTTCCATGCCACACATGTTCCACAAACCGGATGGGATAAGCTGTTTATATCTTTCATACCTGCTGATTCCGGAAAAGCAACTGCAAAAACAACCAAGGCAAACGTGAGGAATGGGACCTGCAAATGGGGAGATCCTATCTATGAAACTACAAGGCTTCTACAAGACACTAAAACCAAACAATACGATGAGAAGCTTTACAAACTTGTGGTAACCATGGGTTCTTCAAGGTCTAGTATCCTTGGCGAGACTAATATCAACCTTGCTGATTATGCTGATGCATCAAAGCCTTCGTCTGTTGCTCTGCCTCTTCTTGGATGTGATTCTGGAACTGTTTTACATGTCACGGTACAGCTGCTAACTTCTAAAACTGGCTTCAGAGAGTTTGAGCATCAGAGGGAGCTCAGAGAGAGCGGTCTACGAACAACCTCTGACCAAAACAGAAATGATGTATCCACTACTAGAAGAATATCATCCTCTGAAGATGCAGTAAATGAGCAGATGGATAAGATGAATTCGAAAGTTAGATTCAAAGAACTCTCTCCCCATGAAGAAGGGGCTGGCTTGAATGAAGAGTATGCTGATTCAACTGGTGGATTTGAATATTCTTCCACTACTTCAGAAAGTTTATATGCTGAGAAACATGACACGTCTAGCACCCATGAGATTGACAGTATTAAGAGTACCGCCTCTGCTGATCTAGGGGGATTGTCCCTTAGTCAAAGTCCCGGACAAGAGAAACAACATCCCTCAGATCAGCGATTCTTGGTACAGGGGACCAGTGAGTGGGCTCATAGCTGGGGCTCTGAATTTTCTCCAGATGCTTATTTACCAAATGCTTCTGAGGAAAATAGCAGGCTTAGAGGAAGCTTGGAAGCAGCTGAATCATCCATTCTTGAGCTTAAGCAGGAGGTGAACTCTTTACAGGCTCATGCTGATGAAATCGGCAATGACGCACAAAAATTCGGTCTTCAACTTGATGCTGAAATTGCTTCTGGAGAACAATTAGCAAAAGAAATTTCCACACTGAGATCCGAATGTTCAAAGTTAAAAGAAGATCTTGAGGAGCAAAAAAGTTTCAAACTAAGCATTCCATTTTCCCACAGGGAAACTATTGAGACAGGCCAAGATTGCATTTTTCATGAGTTATGGCTCAGGTGGTTTAAGGGCCTTTCAAGTATGGAGGATAAATTAAAAGGGCTTCAGAGCAAGGCATTATTTGGAGCAAATGAAAGGGACTTCCAGTCCTGTCACTCGGATTTCGAGGCATTGCTTGGTGTTTTGCAAGTTCTCAAACAAGAGACTGGACAGGCAAGTTCAGGGCTGAACGTGACAAGCGTAAAGCAGGCTGATGAAATAAGTTTACATAAAGGCGAGCAATTAGTACTAGGAACGAGGGTGGATGCAGATTCCTATCAACCCGGGGGTGCGGTTCATTGTCTAAGTATACCTGGCCTGGTGTCACTAGACTTTGATTCTGTAGATGCCGCCAATGCAATGAAAGGGAAGTTTGTTGAAATTTTAAGAGAGTTGGATGAGATGAAAGCTGATCGAGAAAGCCTTGCAAAGAAAGCAGACCAGATGGAGTGCTACTATGAAGCTCTCATTCATGAACTTGAGGAAAACCAGAGACAGATGATTGGAGAATTGCAGAATCTCAGGAATGAGCATTCTACTTGCTTGTACACAATTTCATCTGCTAACGCTGAGATGGAGAGAATCCAGCAAGACATGAACAACGAGCGAAGAATATTTTCCAAAGAAAAGCGTGATTTGGACTCTCTGAACAAGGAGTTTGAAAGAAGGGCTACTACAGCAGAAGCAGCACTTAAAAGGGCACGCATGAATTACTCTATCGCAGTGAACCAGTTACAGAAGGACCTTGAATTGCTTTCATTCCAGGTTCAGTCCATGCATGAGACTAATGAGAACCTCATTAAGCAGGCTTTTGAAGATTCTTTGATTCCAATGTTTCAGGGGTGTGAAGAAACAGTGCAGAATAAGAAATCGGATTCAGAGGAATTTCCATCTGCAAATCACTTACAGTGTCAGAATAAATGCTACGGGATAAAGAAACAGCAGTTAGATGGGGATGCATTATCAGACGATTTGAAAAGATCCCTCCACTTGCAGAAGGGCATCTACCAAAAGGTTGAAGAAGAACTTTATGAGGTGCATCTGGTGAATGTGTATTTGGATGTACTGTCAAAGACTCTACAAGTTACTTTGATTGAAGCAAGTACTGCCGTCGGATTGACGAAAGAGAAAGTGCATGAACTTTCACAACAGCTGGAGCTTTCAACTGAGTCCAATGAGTTGTTGATGCTGAGGCTGCAGACTGCATTGGATGAGATTCGCTGCCTGAATGAGTACAAGGAAACTTGTAATTCAATCCGCAATGACTTGGTTCTGAAGAACCAAATTTTGGAAGCAGATTTACAAAACGCTAGTAGTGAAAATGGCCTTCTTACCCAGAAGATTGTTGAATGGAAAGGTATGATAAAAGAATATGAAACTTATGAGAGCAAATACAAGGCCTGCACTACTGAAAAATTACAGCTGGAAAATTTGTTGCAAAAGGAAACCCTAGAAAACGGCACTCTTCAGAATAAACTTTCCTCTTTGCAGGAAGAGTTGAAATCTGTCAGAACCGACTTTGATGAGCTAGCTTGCATGAAGGAGGATCTGCAGAATATTGTGAATTTTCTACAGGGTAAGTTATGGAATCTGTTGGCATCATATGACCAAAAGTGTAAAAGCCTGGCTCTTTGCGGTGGATGTGTCTGTCAAGACTTGGAATCCAGAGATTTAACAGGTGTAGTGATGCAAATAGAAGAGCTTCAGAACAATGTATATGGAAAGATTGTCCAAATGATGGAAGAAAAGAAAGAGCTAGCGCAAGAAAGAGATATTGCTCAAGAGTCTTTACGCGCAGCAGAGTCTGATAACCTGATCATGAAGCAGAAATTTGAACACGATCTACGAGGTACAGTGGATAAACTAGATGTTTCGAGTGCGCTGGTTCAAAAGCTCCAGTCACGAGTTGAGGCCATGGCAAACAGACCCGAGATTAGCTGTGAAGCCGAAGACAATTATGCACAACAACACAGAGAGCTTTTGGTTGGTCTTGATAATTTAGAATTGGAGCTGCAGCAACTTACTTCTAAAATTGAGGGCCTTGCTGAGGAATTTATGGCATTGGAGAAATTAACTGAACAACTAGGAAGGTGTAACCTGACCATAGCAGCATTAACAGTGGAAAAAGAAGCTTTGATGGTGTCCTTACAGGATAAAACTGATGAATCTTCCAGGCTTGCTCTCGTGCTTAATAGTTTGCAAGGGAGTTTGCTTTCTTTGCATGATGAGGTGCAAACAGAGAGAAATCTCAGAGATAAGTTAGAGAGTACAATCGCAGATCTCACATCCCAATTGAACGAGAAGCATTGCCAGGTGCTAGGTTTTGATCAGCAGAAGGCTGAGCTTGTCCATCTCAAACAATTGGTTTCAGATCTAGAATTAGAGAAATCAAGAGTCTCCCGTCTCTTGTTGGATTCTGAGGAATATCTAAAAGACGTCCGTGAAGAATGTTCTTCCGTTTCTGCTTTAGAGGCTCAATTGTCTGAAATGCATGAATTTTCAATAGCTGCTGATGTTGGACTCACTTTCACAAAAGCTCAATATGAGACAAGGATAGAAGAACTAGAAAGGTATAACTTGACCGTTGCAGCACTGTTAGAGGAAAAAGAAGTTTTGATGGCGTCTTTACAGGATAAAACTCAAGAATCTTCCAGGCTTGCTCTCGAGCTTAATAGTTTGCAAGGGAGTTTGGTTTCTTTGCATGATGAGGTGCAAACAGAAAGAAATCTAAGGGATAGGTTAGAGAGTACAATTACAGAGCTCACTTCCCATTTGAATGAGAAGCATTGCCAGTTGCTAGGTTTTGATCAGCAGAAGGCTGAGCTTGCCCATCTAAAACAATTGGTATTGGATCTAGAACTAGAGAAATCAAGAGTTTCCTGTCTCTTGTTGGATTCTGAGGAATGTCTAAAAGACGTCCGTAAAGAGTGTTCTTCCATTTCTGCTTTGGAGGCTCAATTGTCTGAATTGCATGAATCTTCAATAGCTGCTGACGTTGGACTCACTTTCACAAAAGTTCAATATGAGATGAGGATTGAAGAACTAGAAAGGTATAACCTGACCATAGCAGAATTATCAGAGGAGAAAGAAGCTTTAATGGCGTCCTTGCAGAATAAAAATGAAGAATCTTCCAAGCTTTGTCTGGAGCTTAATAGGATGCAAGGAAGTTTGCTCTCTTTGCAAGATGAGTTGCAAACTGAGAGAAATCTCAGAGATAAGTCAGAGAGTAGAATTACAGATCTGGCTTCCCAATTGATTAAGAAGAACAGTCAGTTGCTAGATTTTGATCACCGGATGGCTGAGCTGGTCCATCTCAAAGAACTGGTATCGGACCTAGAACTAGAGAAATCAAGAGTTCTCGGTCTCCTGTTGGATTCTGAGAAATGTCTTAAAGACGCTCATGAAGAGTGTTCTTCTGTCTCTGCTTTGGAAGCTCAGTTATCTGAAATGCATGAATTTTCAATAGCCGCTGATGTTGGACTCACTTTCACAAAAACTCAATACGAGGCCGTGATCGAAGAGCTTTGTCAGAAACTGCACTTTTCAGATAGCCAAGTTTCTGAAATTCGCAACAATTTCCTTAATGTAGAGAATATGCTTAACAAATGTCTTGCTAGCGAAAGGCATTACCTTGAAGAGAACACCCAATTGATGACAAGTCTCAATTTCATTAAATCTGAGTTGGAAGCCTCCAGTGCTCAAAACAGGATACTTCTTGATGCAAACAGTGTTATGAGGACCGAACTTGAGGAAAACAAGAAAAGAGCTGAAAATATGGAGGATATTGTCCATATGGATAAAAGTCAGTCTGCTCTTGAGGTGGAAAGGCTGGAACATCTTCTAATGACTTCTGAAGAAGAGATTGATAACCTGATATTCTCCAAGGAAGAACTGGAAGTCAAAGCTTTAGTACTCAAAGCCAAGTTGGATGAACAGTCTGCTCAGCTAACCTTGCTGGAAGGATATAAGAATGAAATGGAAATGCTGCACGATAGATGTAGCGATCTAACACAGAAGCTTGCTCAACAGGTCTTAAAGACGGAAGAATTTAAGAACTTGTCCATCCACTTTAAGGATCTTAAAGACAAGGCTTATGCCGAGGGCCTCCATGCACAGGGTAAAAAAGAACCAGAAAGGCCACCAACTGCTATGCAAGAGTCTTTAAGAATTGTATTCATCAAAGAACAATATGAAACCAAGCTGCAAGAACTGAAACAACAACTTGCTATGTCCAATAAGCACAGTGAGGAGATGCTTTGGAAATTACAAGATGCAGTCGACGAAGTTGAGAATAGGAAAAAATCCGAAGCTACTCATGTGAAGAGAAACGAAGAGCTAGGAATGAAGATCTTGGAATTGGAGTCTGACCTACATTCATTACTTTCTGAAAAGCGTGAAATAATGAAAGCATATGACCTGATGAAAGCTGAAAAGGAATGCTCATTAATAAGCCTTGAGTGCTGTAAGGAAGAAAAACAAGAGCTTGAAGCATCTTTGCAGAAGTGCACTGAGGAAAAAGCTAAAATTGCTTTAGAACTCACCTCAGCGAAGGATTTACTTTCGAGCAGTTCATCATCTGTAAATTATCAGAAGGGCGCAGAGGAACCTTTGGTTAAGTTTTCTGAACTAGATGGAGCCAATGGTGAAGCATCTCAGCATGAATGCATGAGTTCCGTTGATGAAGCAGACCAGTCAAATGTACTCAACAATATAAACTCCAAGCAGGATGATCTTGTATCCGGAGGTGTAAATGGGATCTCTGGTATAGTACTTTCAAAGCAAAGGGATATTCTTAATAGCGACATGAAGCATTTGGTTCTTGCCAATGAGAATTTTAAGGCCCAAGGCTTAAAGTCTAGCATGGAAAACTTAAATAAGGAGTTGGAAAGGATGAAACATGAAAATTTGCTTCTTTCCGTCGATGATCAACACCTTGAACCAAATTTTCCTGGTTTACATAGAGAGATTATGCAATTAAATAAGGTAAATGAAGAATTGGGAAATATATTTCCCTCCTTCAACGAATTTTCTTGCAGTGGCAATGCATTAGAGAGGGTGCTAGCTTTGGAAGTTGAACTTGCTGAAGCATTGCAGGCAAAGAAGAAATCAACTATCCAGTTTCAAAGTTCTTTCGTGAAACAACACAGTGATGAGGAAGCAGTGTTTCACAGCTTCAGAGACATCAACGAGCTAATCAAAGACATGCTGGAGATTAAGGGAAGATATGCTACCGTGGAAACGGAACTCAAAGACATGCATGACCGTTACTCCCAGTTAAGCCTTCAATTTGCAGAGGTCGAAGGAGAGAGACAAAAACTAATGATGACTCTCAAGAATGTCCGAGCGTCAAAGAAAGCCCTATACTTGAATCGCTCCTCAACATCCCCTTTTCTGGACCCTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

2201

Amino Acids

249.52

Weight (kDa)

4.94

Isoelectric Point (pI)

49.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 141 3.6e-26 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 3636, 4116
Acc36I ACCTGC 2 cut(s) 130, 188
AccB7I CCANNNNNTGG 3 cut(s) 846, 2485, 2935
AccBSI CCGCTC 1 cut(s) 474
AccI GTMKAC 3 cut(s) 478, 1507, 5475
AccII CGCG 1 cut(s) 2991
AccIII TCCGGA 2 cut(s) 131, 5936
AciI CCGC 7 cut(s) 474, 747, 1490, 1548, 2455, 2832, 4665
AclWI GGATC 8 cut(s) 17, 188, 1086, 2172, 2751, 4056, 5280, 5960
AcyI GRCGYC 4 cut(s) 3633, 3818, 4113, 4298
AdeI CACNNNGTG 1 cut(s) 18
AflII CTTAAG 1 cut(s) 962
AflIII ACRYGT 3 cut(s) 76, 400, 708
AhdI GACNNNNNGTC 1 cut(s) 1303
AjiI CACGTC 1 cut(s) 711
AjnI CCWGG 8 cut(s) 1510, 1515, 1959, 2820, 3368, 3507, 3848, 6145
AjuI GAANNNNNNNTTGG 4 cut(s) 2237, 2269, 2468, 2500
Alw21I GWGCWC 4 cut(s) 465, 3960, 4897, 5705
Alw44I GTGCAC 1 cut(s) 5701
AlwI GGATC 8 cut(s) 17, 188, 1086, 2172, 2751, 4056, 5280, 5960
AlwNI CAGNNNCTG 2 cut(s) 3002, 4732
Ama87I CYCGRG 3 cut(s) 1481, 3125, 3860
Aor13HI TCCGGA 2 cut(s) 131, 5936
ApaLI GTGCAC 1 cut(s) 5701
ArsI GACNNNNNNTTYG 4 cut(s) 338, 370, 1012, 1044
AseI ATTAAT 1 cut(s) 5642
Asp700I GAANNNNTTC 3 cut(s) 1813, 2898, 5034
AspLEI GCGC 4 cut(s) 2959, 2993, 3079, 5795
AsuC2I CCSGG 3 cut(s) 789, 1482, 1483
AsuHPI GGTGA 5 cut(s) 985, 2254, 4478, 5728, 5852
AsuII TTCGAA 1 cut(s) 578
AsuNHI GCTAGC 4 cut(s) 2725, 2953, 4811, 6256
AvaI CYCGRG 3 cut(s) 1481, 3125, 3860
AvaII GGWCC 7 cut(s) 177, 837, 1939, 4504, 4528, 4935, 6592
BaeGI GKGCMC 2 cut(s) 1900, 5705
BanII GRGCYC 4 cut(s) 465, 853, 866, 3960
BarI GAAGNNNNNNTAC 8 cut(s) 1855, 1887, 2186, 2218, 2481, 2513, 6409, 6441
BauI CACGAG 2 cut(s) 3099, 3380
BbsI GAAGAC 3 cut(s) 38, 1022, 3153
Bbv12I GWGCWC 4 cut(s) 465, 3960, 4897, 5705
BbvCI CCTCAGC 3 cut(s) 2387, 3243, 5740
BceAI ACGGC 3 cut(s) 2291, 2671, 4691
BciT130I CCWGG 8 cut(s) 1512, 1517, 1961, 2822, 3370, 3509, 3850, 6147
BciVI GTATCC 4 cut(s) 308, 523, 4899, 5944
BclI TGATCA 5 cut(s) 3012, 3523, 4003, 4483, 6121
BcnI CCSGG 3 cut(s) 789, 1482, 1483
BfmI CTRYAG 6 cut(s) 1539, 1872, 2393, 2747, 2768, 3209
BfrI CTTAAG 1 cut(s) 962
BfuAI ACCTGC 2 cut(s) 130, 188
BfuI GTATCC 4 cut(s) 308, 523, 4899, 5944
BglII AGATCT 5 cut(s) 1117, 3475, 3568, 4435, 5544
BlpI GCTNAGC 4 cut(s) 3537, 4017, 4497, 5139
BmcAI AGTACT 4 cut(s) 1446, 2302, 5109, 5967
Bme18I GGWCC 7 cut(s) 177, 837, 1939, 4504, 4528, 4935, 6592
BmeRI GACNNNNNGTC 1 cut(s) 1303
BmeT110I CYCGRG 3 cut(s) 1481, 3125, 3860
BmgBI CACGTC 1 cut(s) 711
BmiI GGNNCC 5 cut(s) 178, 838, 5802, 5833, 6594
BmrI ACTGGG 1 cut(s) 6463
BmtI GCTAGC 4 cut(s) 2729, 2957, 4815, 6260
BmuI ACTGGG 1 cut(s) 6463
BpiI GAAGAC 3 cut(s) 38, 1022, 3153
BplI GAGNNNNNCTC 2 cut(s) 962, 994
BpmI CTGGAG 7 cut(s) 861, 1077, 2372, 3077, 4361, 4873, 6425
Bpu10I CCTNAGC 4 cut(s) 1214, 2387, 3243, 5740
Bpu1102I GCTNAGC 4 cut(s) 3537, 4017, 4497, 5139
Bpu14I TTCGAA 1 cut(s) 578
BpuMI CCSGG 3 cut(s) 789, 1482, 1483
BsaBI GATNNNNATC 2 cut(s) 1473, 4482
BsaHI GRCGYC 4 cut(s) 3633, 3818, 4113, 4298
BsaI GGTCTC 1 cut(s) 4565
BsaWI WCCGGW 4 cut(s) 89, 131, 4488, 5936
BsaXI ACNNNNNCTCC 2 cut(s) 6449, 6479
Bse3DI GCAATG 8 cut(s) 1015, 1328, 1563, 2464, 3502, 3982, 6244, 6284
Bse8I GATNNNNATC 2 cut(s) 1473, 4482
BseAI TCCGGA 2 cut(s) 131, 5936
BseBI CCWGG 8 cut(s) 1512, 1517, 1961, 2822, 3370, 3509, 3850, 6147
BseJI GATNNNNATC 2 cut(s) 1473, 4482
BseMI GCAATG 8 cut(s) 1015, 1328, 1563, 2464, 3502, 3982, 6244, 6284
BseRI GAGGAG 4 cut(s) 1139, 4292, 5459, 6562
BseSI GKGCMC 2 cut(s) 1900, 5705
BseYI CCCAGC 1 cut(s) 858
BsgI GTGCAG 4 cut(s) 2075, 2584, 4718, 5168
Bsh1236I CGCG 1 cut(s) 2991
BsiHKAI GWGCWC 4 cut(s) 465, 3960, 4897, 5705
BsiHKCI CYCGRG 3 cut(s) 1481, 3125, 3860
BsiSI CCGG 6 cut(s) 90, 132, 789, 1482, 4489, 5937
BslFI GGGAC 5 cut(s) 190, 757, 771, 850, 1310
BsmBI CGTCTC 1 cut(s) 3605
BsmFI GGGAC 5 cut(s) 190, 757, 771, 850, 1310
BsmI GAATGC 4 cut(s) 1149, 1729, 5639, 5864
Bso31I GGTCTC 1 cut(s) 4565
BsoBI CYCGRG 3 cut(s) 1481, 3125, 3860
Bsp119I TTCGAA 1 cut(s) 578
Bsp1286I GDGCHC 7 cut(s) 465, 853, 866, 1900, 3960, 4897, 5705
Bsp13I TCCGGA 2 cut(s) 131, 5936
Bsp1407I TGTACA 1 cut(s) 1743
Bsp1720I GCTNAGC 4 cut(s) 3537, 4017, 4497, 5139
Bsp19I CCATGG 2 cut(s) 276, 3111
BspACI CCGC 7 cut(s) 474, 747, 1490, 1548, 2455, 2832, 4665
BspEI TCCGGA 2 cut(s) 131, 5936
BspFNI CGCG 1 cut(s) 2991
BspHI TCATGA 4 cut(s) 1201, 1669, 3015, 4597
BspLI GGNNCC 5 cut(s) 178, 838, 5802, 5833, 6594
BspMAI CTGCAG 3 cut(s) 2397, 2751, 3213
BspMI ACCTGC 2 cut(s) 130, 188
BspOI GCTAGC 4 cut(s) 2729, 2957, 4815, 6260
BspPI GGATC 8 cut(s) 17, 188, 1086, 2172, 2751, 4056, 5280, 5960
BspQI GCTCTTC 3 cut(s) 2889, 4710, 5523
BspT104I TTCGAA 1 cut(s) 578
BspTI CTTAAG 1 cut(s) 962
BspTNI GGTCTC 1 cut(s) 4565
BsrBI CCGCTC 1 cut(s) 474
BsrDI GCAATG 8 cut(s) 1015, 1328, 1563, 2464, 3502, 3982, 6244, 6284
BsrGI TGTACA 1 cut(s) 1743
BssNAI GTATAC 1 cut(s) 1508
BssNI GRCGYC 4 cut(s) 3633, 3818, 4113, 4298
BssSI CACGAG 2 cut(s) 3099, 3380
BssT1I CCWWGG 6 cut(s) 156, 276, 301, 3111, 5079, 6040
Bst1107I GTATAC 1 cut(s) 1508
Bst2BI CACGAG 2 cut(s) 3099, 3380
Bst2UI CCWGG 8 cut(s) 1512, 1517, 1961, 2822, 3370, 3509, 3850, 6147
BstACI GRCGYC 4 cut(s) 3633, 3818, 4113, 4298
BstAFI CTTAAG 1 cut(s) 962
BstAPI GCANNNNNTGC 1 cut(s) 2303
BstAUI TGTACA 1 cut(s) 1743
BstBI TTCGAA 1 cut(s) 578
BstDSI CCRYGG 3 cut(s) 276, 3111, 6429
BstEII GGTNACC 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 3242
BstFNI CGCG 1 cut(s) 2991
BstHHI GCGC 4 cut(s) 2959, 2993, 3079, 5795
BstNI CCWGG 8 cut(s) 1512, 1517, 1961, 2822, 3370, 3509, 3850, 6147
BstNSI RCATGY 4 cut(s) 80, 404, 6403, 6454
BstPI GGTNACC 1 cut(s) 271
BstSFI CTRYAG 6 cut(s) 1539, 1872, 2393, 2747, 2768, 3209
BstSLI GKGCMC 2 cut(s) 1900, 5705
BstUI CGCG 1 cut(s) 2991
BstV2I GAAGAC 3 cut(s) 38, 1022, 3153
BstZ17I GTATAC 1 cut(s) 1508
BsuI GTATCC 4 cut(s) 308, 523, 4899, 5944
BtgI CCRYGG 3 cut(s) 276, 3111, 6429
BtrI CACGTC 1 cut(s) 711
BtsI GCAGTG 3 cut(s) 1926, 6238, 6363
BveI ACCTGC 2 cut(s) 130, 188
CaiI CAGNNNCTG 2 cut(s) 3002, 4732
CciI TCATGA 4 cut(s) 1201, 1669, 3015, 4597
CfoI GCGC 4 cut(s) 2959, 2993, 3079, 5795
Cfr9I CCCGGG 1 cut(s) 1481
CseI GACGC 5 cut(s) 1022, 3807, 4287, 4601, 6531
DraIII CACNNNGTG 1 cut(s) 18
DriI GACNNNNNGTC 1 cut(s) 1303
Eam1105I GACNNNNNGTC 1 cut(s) 1303
Ecl136II GAGCTC 2 cut(s) 463, 3958
Eco130I CCWWGG 6 cut(s) 156, 276, 301, 3111, 5079, 6040
Eco147I AGGCCT 1 cut(s) 2597
Eco24I GRGCYC 4 cut(s) 465, 853, 866, 3960
Eco31I GGTCTC 1 cut(s) 4565
Eco47I GGWCC 7 cut(s) 177, 837, 1939, 4504, 4528, 4935, 6592
Eco53kI GAGCTC 2 cut(s) 463, 3958
Eco88I CYCGRG 3 cut(s) 1481, 3125, 3860
Eco91I GGTNACC 1 cut(s) 271
EcoICRI GAGCTC 2 cut(s) 463, 3958
EcoNI CCTNNNNNAGG 1 cut(s) 3242
EcoO109I RGGNCCY 5 cut(s) 177, 1228, 1939, 3235, 5299
EcoO65I GGTNACC 1 cut(s) 271
EcoRI GAATTC 1 cut(s) 574
EcoRII CCWGG 8 cut(s) 1510, 1515, 1959, 2820, 3368, 3507, 3848, 6145
EcoT14I CCWWGG 6 cut(s) 156, 276, 301, 3111, 5079, 6040
EcoT22I ATGCAT 8 cut(s) 343, 1977, 2158, 3690, 4650, 5864, 6244, 6456
EcoT38I GRGCYC 4 cut(s) 465, 853, 866, 3960
ErhI CCWWGG 6 cut(s) 156, 276, 301, 3111, 5079, 6040
Esp3I CGTCTC 1 cut(s) 3605
FalI AAGNNNNNCTT 8 cut(s) 1104, 1136, 1872, 1904, 2208, 2240, 6258, 6290
FaqI GGGAC 5 cut(s) 190, 757, 771, 850, 1310
FauNDI CATATG 3 cut(s) 2800, 4989, 5608
FbaI TGATCA 5 cut(s) 3012, 3523, 4003, 4483, 6121
FblI GTMKAC 3 cut(s) 478, 1507, 5475
FriOI GRGCYC 4 cut(s) 465, 853, 866, 3960
GlaI GCGC 4 cut(s) 2958, 2992, 3078, 5794
GsaI CCCAGC 1 cut(s) 862
GsuI CTGGAG 7 cut(s) 861, 1077, 2372, 3077, 4361, 4873, 6425
HapII CCGG 6 cut(s) 90, 132, 789, 1482, 4489, 5937
HgaI GACGC 5 cut(s) 1022, 3807, 4287, 4601, 6531
HhaI GCGC 4 cut(s) 2959, 2993, 3079, 5795
Hin1I GRCGYC 4 cut(s) 3633, 3818, 4113, 4298
Hin6I GCGC 4 cut(s) 2957, 2991, 3077, 5793
HinP1I GCGC 4 cut(s) 2957, 2991, 3077, 5793
HincII GTYRAC 1 cut(s) 5476
HindII GTYRAC 1 cut(s) 5476
HindIII AAGCTT 8 cut(s) 23, 254, 928, 3327, 4287, 4331, 5100, 5213
HpaII CCGG 6 cut(s) 90, 132, 789, 1482, 4489, 5937
HphI GGTGA 5 cut(s) 985, 2254, 4478, 5728, 5852
Hpy166II GTNNAC 9 cut(s) 479, 976, 1424, 1508, 1746, 1924, 5476, 5703, 6152
Hpy8I GTNNAC 9 cut(s) 479, 976, 1424, 1508, 1746, 1924, 5476, 5703, 6152
Hpy99I CGWCG 3 cut(s) 2312, 5480, 6119
HpyCH4IV ACGT 6 cut(s) 165, 710, 1389, 3633, 4113, 4191
HpySE526I ACGT 6 cut(s) 165, 710, 1389, 3633, 4113, 4191
Hsp92I GRCGYC 4 cut(s) 3633, 3818, 4113, 4298
HspAI GCGC 4 cut(s) 2957, 2991, 3077, 5793
Kpn2I TCCGGA 2 cut(s) 131, 5936
Ksp22I TGATCA 5 cut(s) 3012, 3523, 4003, 4483, 6121
LguI GCTCTTC 3 cut(s) 2889, 4710, 5523
LmnI GCTCC 9 cut(s) 460, 1126, 1283, 2353, 3096, 3205, 4342, 5831, 6575
MaeII ACGT 6 cut(s) 165, 710, 1389, 3633, 4113, 4191
MbiI CCGCTC 1 cut(s) 474
MfeI CAATTG 7 cut(s) 3488, 3557, 3674, 4037, 4154, 4448, 4844
MhlI GDGCHC 7 cut(s) 465, 853, 866, 1900, 3960, 4897, 5705
Mph1103I ATGCAT 8 cut(s) 343, 1977, 2158, 3690, 4650, 5864, 6244, 6456
MroI TCCGGA 2 cut(s) 131, 5936
MroXI GAANNNNTTC 3 cut(s) 1813, 2898, 5034
MslI CAYNNNNRTG 3 cut(s) 996, 3972, 6345
MspA1I CMGCKG 5 cut(s) 415, 941, 2350, 2620, 4667
MspCI CTTAAG 1 cut(s) 962
MspI CCGG 6 cut(s) 90, 132, 789, 1482, 4489, 5937
MunI CAATTG 7 cut(s) 3488, 3557, 3674, 4037, 4154, 4448, 4844
Mva1269I GAATGC 4 cut(s) 1149, 1729, 5639, 5864
MvaI CCWGG 8 cut(s) 1512, 1517, 1961, 2822, 3370, 3509, 3850, 6147
MvnI CGCG 1 cut(s) 2991
NciI CCSGG 3 cut(s) 789, 1482, 1483
NcoI CCATGG 2 cut(s) 276, 3111
NdeI CATATG 3 cut(s) 2800, 4989, 5608
NheI GCTAGC 4 cut(s) 2725, 2953, 4811, 6256
NlaIV GGNNCC 5 cut(s) 178, 838, 5802, 5833, 6594
NmeAIII GCCGAG 1 cut(s) 5320
NmuCI GTSAC 5 cut(s) 403, 1310, 1390, 1521, 3096
NsiI ATGCAT 8 cut(s) 343, 1977, 2158, 3690, 4650, 5864, 6244, 6456
NspI RCATGY 4 cut(s) 80, 404, 6403, 6454
NspV TTCGAA 1 cut(s) 578
PaeR7I CTCGAG 1 cut(s) 3860
PagI TCATGA 4 cut(s) 1201, 1669, 3015, 4597
PceI AGGCCT 1 cut(s) 2597
PciI ACATGT 2 cut(s) 76, 400
PciSI GCTCTTC 3 cut(s) 2889, 4710, 5523
PcsI WCGNNNNNNNCGW 1 cut(s) 1395
PctI GAATGC 4 cut(s) 1149, 1729, 5639, 5864
PdmI GAANNNNTTC 3 cut(s) 1813, 2898, 5034
PflMI CCANNNNNTGG 3 cut(s) 846, 2485, 2935
PfoI TCCNGGA 1 cut(s) 787
PpuMI RGGWCCY 2 cut(s) 177, 1939
PscI ACATGT 2 cut(s) 76, 400
PshBI ATTAAT 1 cut(s) 5642
Psp124BI GAGCTC 2 cut(s) 465, 3960
Psp5II RGGWCCY 2 cut(s) 177, 1939
Psp6I CCWGG 8 cut(s) 1510, 1515, 1959, 2820, 3368, 3507, 3848, 6145
PspEI GGTNACC 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 858
PspGI CCWGG 8 cut(s) 1510, 1515, 1959, 2820, 3368, 3507, 3848, 6145
PspN4I GGNNCC 5 cut(s) 178, 838, 5802, 5833, 6594
PspPPI RGGWCCY 2 cut(s) 177, 1939
PsrI GAACNNNNNNTAC 4 cut(s) 3024, 3056, 4111, 4143
PstI CTGCAG 3 cut(s) 2397, 2751, 3213
PstNI CAGNNNCTG 2 cut(s) 3002, 4732
PvuII CAGCTG 4 cut(s) 415, 941, 2350, 2620
RseI CAYNNNNRTG 3 cut(s) 996, 3972, 6345
SacI GAGCTC 2 cut(s) 465, 3960
SalI GTCGAC 1 cut(s) 5474
SapI GCTCTTC 3 cut(s) 2889, 4710, 5523
ScaI AGTACT 4 cut(s) 1446, 2302, 5109, 5967
SduI GDGCHC 7 cut(s) 465, 853, 866, 1900, 3960, 4897, 5705
SfcI CTRYAG 6 cut(s) 1539, 1872, 2393, 2747, 2768, 3209
Sfr274I CTCGAG 1 cut(s) 3860
SfuI TTCGAA 1 cut(s) 578
SinI GGWCC 7 cut(s) 177, 837, 1939, 4504, 4528, 4935, 6592
SlaI CTCGAG 1 cut(s) 3860
SmaI CCCGGG 1 cut(s) 1483
SmiMI CAYNNNNRTG 3 cut(s) 996, 3972, 6345
SseBI AGGCCT 1 cut(s) 2597
SsiI CCGC 7 cut(s) 474, 747, 1490, 1548, 2455, 2832, 4665
SspI AATATT 3 cut(s) 665, 1812, 2755
SstI GAGCTC 2 cut(s) 465, 3960
StuI AGGCCT 1 cut(s) 2597
StyI CCWWGG 6 cut(s) 156, 276, 301, 3111, 5079, 6040
TaiI ACGT 6 cut(s) 168, 713, 1392, 3636, 4116, 4194
TaqII GACCGA 3 cut(s) 1016, 4547, 4952
TauI GCSGC 2 cut(s) 1550, 4667
TseFI GTSAC 5 cut(s) 403, 1310, 1390, 1521, 3096
Tsp45I GTSAC 5 cut(s) 403, 1310, 1390, 1521, 3096
TspGWI ACGGA 7 cut(s) 3626, 3643, 4106, 5252, 5860, 6103, 6452
TspMI CCCGGG 1 cut(s) 1481
Van91I CCANNNNNTGG 3 cut(s) 846, 2485, 2935
Vha464I CTTAAG 1 cut(s) 962
VneI GTGCAC 1 cut(s) 5701
VpaK11BI GGWCC 7 cut(s) 177, 837, 1939, 4504, 4528, 4935, 6592
VspI ATTAAT 1 cut(s) 5642
XagI CCTNNNNNAGG 1 cut(s) 3242
XbaI TCTAGA 2 cut(s) 3571, 4051
XceI RCATGY 4 cut(s) 80, 404, 6403, 6454
XcmI CCANNNNNNNNNTGG 4 cut(s) 90, 4141, 4336, 5086
XhoI CTCGAG 1 cut(s) 3860
XmaI CCCGGG 1 cut(s) 1481
XmiI GTMKAC 3 cut(s) 478, 1507, 5475
XmnI GAANNNNTTC 3 cut(s) 1813, 2898, 5034
ZraI GACGTC 2 cut(s) 3634, 4114
ZrmI AGTACT 4 cut(s) 1446, 2302, 5109, 5967
Zsp2I ATGCAT 8 cut(s) 343, 1977, 2158, 3690, 4650, 5864, 6244, 6456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.