MD15G1361900.v1.1

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003440
Physical Location & Seq
Forward (+)
43645448 .. 43656211
10764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1361900.v1.1.491

Sequence Viewer

Length: 6606 bp
ATGTCAAGGGTCACTAGGTGGAAGCTTGAGAAGACAAAAGTCAAAGTGGTTTTCAGGCTACAATTCCATGCCACAAATGTTCCACAAACCGGATGGGATAAGCTGTTTATATCTTTCATACCTGCTGATTCCGGAAAAGCAACTGCAAAAACTACCAAGGCAAATGTAAGGAATGGGACCTGTAAATGGGGAGATCCTATCTATGAAACTACAAGACTTCTACAAGACACTAAAACCAAACAATACGATGAGAAGCTTTACAAACTTGTGGTAACCATGGGTTCTTCAAGGTCTAGTATCCTTGGCGAGACTAATATCAACCTTGCTGATTATGCTGATGCTTCAAAGCCTTCGTCTGTTGCACTTCTACTCCTTGGATGTGATTCTGGAACTGTTTTACATGTCACAGTGCAGCTGCTAACTTCTAAAACTGGCTTCAGAGAGTTTGAGCAGCAGAGGGAGCTCAGAGAGAGTGGTCTACGAACAACCTCTGACCAAAATAGAAATGATGTATCCACTACTAGAAAGATATCATCTTCTGAAGATACAGTGAATGATCAGATGGATAAGATGAATTCGAAGGTTAAATTCAAAGAACTTTCTCCCCTTGAAGAGGGGGCTGGCCCGAATGAAGAGTATGCAGATTCAACTGGTGGATTTGAATGTTCTTCCAATACTTCAGAAAGTTTATATGCTGAGAAACATGACACGTCTAGCACCCATGAGATTGATAGTATTAAGAGTACCGCCTCTGGTGATTTAGGGGGATTGTCCCTTAGTCAAAGTCCTGGACAAGAGAAACGACATCCCACTGATCAGCGATTCTTGGCACAGGGAACCAGCGAGTGGGCTCATAGCTGGGGTTCTGACTTTTCTGCAGATGCTGATTTGCCAAATACTTATGAGGAAAATAGTAGGCTTAGAGGAAGCTTGGAAGCTGCTGAATTATCCATTCTTGAGCTTAAGCAGGAGGTGAGCTCTTTACAGCTTCATGCTGATGAAATAGGCAATGACGCTAAAAAAATTTCTTTTCAACTTGATGCTGAAATTGCTTCTGGAGAACAATTAGCAAAAGAAGTTTCCGTACTGAGATCCGAATGTTCAAAGTTAAAAGAAGATCTTGAGGAGCAAAAAAGTTTCAATTTAACCATTCCATTCTCCCGCAGAGAAAGTATTGAGACAAGCCAAGTTCACATTTTTCATGAGTTACACCTCAGGTGGTTTAAGGGGCTTTCAAGTATGGAAGATAAATTAAAAGAGATTCAGAGGAAGGCAATCTTGGGAGTAAATGAAAGGAACTTCCAGTCCTGTCACTCGGATTTCGAGGCATTGCTTTGTGTTTTGCTAGTTCTCAAACAAGAGACTGGGCAGGCAAGTTCAGGGATGAACATGACAAGCGTAAACCAGGCTGATGATATGACTTTACATAAAGGTGAGCAATTAGTACTAGGAACAAGGGTGGATGCAGATTTCTATAAACTCAAGGGTGCGGTTCATTGTATAAGCATACCTGGCCTGGTGTCGCAAGACTTTGATACTGTAGATGCTGCCAATGCAATGAAAGGGAAATTTGTTGAACTTTTAAGGGAGTTGGATGAGTTGAGAGCTGATCGAGAAAGCCTTGCAAAGAAAGCAGACCAGATGGAATGCTACTATGAAGCTCTCATTCATGAACTTGAGGAAAACCAGAGACAGATGATGGGAGAATTGCAGAGTCTCAGAAATGAGCATTCTACTTGCATGTACACAATTTCATCTGCTAACTCTGAGATGGAGAGAATCCAGCAAGACATGAACAACGAGCGAATAATATTTTCCAAAGAAAAGTGTGATTTGGACTCTCTGAACAAGGAGTTTGAAAGAAGGGCTACTACAGCAGAAGCAGCTCTTAAAAGGGCATGCATGAATTACTCTATTGCAGTGAACCAGTTACAGAAGGACCTTGAATTGCTTTCTTTCCAGGTTCAGTCCATGCATGAGACTAATGAGAACCTCATTAAGCAGGCTTTTGAAGATTCTTTGATTCCAAGGTTTCAGGAGTGTGAAGAAACAGTGCAGAATCGGAAATCAGATTCAGAGGATTTTCTGTCAGCAAAATATTTGCAGTGTCAGAATAAATGTTACAAGACAAAGAATCAGAAGTTAGATGGGGATGCATTATCAAACGATTTGAGAAGATCCCTCCTCTTACAGAAGGGGCTCTACCAAAAGATTGAAGAAGAACTTTATGAGGTGCATCTGGTGAATGTGTATTTGGATGTATTCTCAAAGACTCTACAAGTTACTTTGGTTGAAGCAAGTGCTGACTTCGGATTGACAAAAGAGAGAATGCATGAACTTTCACACCAGCTGGAGCTTTCAACTGAGTCCAATGAGTTATTGATGCTGAGGCTGCAGACTGCATTGGATGAGATTCGCTGCCTGAATGAATACAAGGAAACTTGTAATTCGATCCGCAATGACTTGGCTCTGAAGAACCAAATTTTGGAAGCAGATTTACAAAACACTACCAGTGAAAATGGTCTTCTTACCCAGAAGATTGTTGAATGGAAAGGTATGATAAAAGAATATGAAACTTATGAGAGCAAATACAAGGCCTGCACTACTGAAAAATTACAGCTGGAAAATTTGTTGCAAAAGGAAACCTTAGAAAACGACACTCTACAAAATAGACTTTCCTCTTTGCGGGAAGAGTTGAAATCTGTCAGAATTGACTTTGATGAGCTTGCTTGCACGAAGGAGGATCTGCAGAATATTGTAAACTTTCTACAGGGTAAGTTATGGAATCTGTTGGCATCTTATGACCTAAAGTATAAAAGCCTGGCTCCTTGCGGTGGATCTGTCTGTCAAGACTTGGAATCCAAAGATTTAACAGGTGTACTGTTGCAAATAGAAGAGTTTCAGAACAAGGTATATGAAAAGACTGTCCAGATGATTGAAGAGAAGAAAGACCTAGTGCAAGAAAGAGATATTGCTCAAGAGTCTTTACGCGCAGCAGAATCTGATAATCTGATGATGAAGCAGAAATTTGAACATGATCTACGAGGTACAGTGGATAAACTAGATGTTTTGAGTGCGCTGGTTCACAAGCTCCAGTTACGAGTTGAGGCCATTGCAAACAGACCCGAGATTAGCTCTGAAGCTGAAGACAATTATGCACAACAGCACAGAGAGCTTTTGTCTGATCTTGATAATTTAGAAATGGAGCTCCAGCAACTTACTTCTAAAAACGAGGGCCTTGCTGAGGAATTTATGGCATTAGAGAAAGTAACTGAAGAATTAGCAAGGTGTAACCTGGCCATAGCAGCATTAACAGAGGAAAAAGAAGCTTTGATGGTGTTCTTACAGGATAAAACTGAAGAATCTTCCAGGCTTGCTCTCAAGCTTAATAATTTGCAAGGGAGTTTACTTTCTTTGCATGATGAGGTCCACACAGAGAGAAATCTCAGAGATAAGTTAGAGAGTAAAATTACAGATCTCACTTCCCAATTGAACGAGAAGCATTGCCAGTTGCTAGGTTTTGATCAGCAGAAGGCTGAGCTGGTCCATCTCAAACAATCGGTATCCGATCTAGAACTGGAGAAATCAAGAGTTTCCTGTCTCCTGTTGGACTCTGACGAATGTATAAAAGATTTAGAAGCTCAATTGTTTGAAATGCATGAATTTTCGATAGCTACTGATGTTGGACTGTTTTTCACAAAGGCTCAATATGAGACCAGGATTGAAGAACTAGGAAGGTGTAACCTCACCATAGCAGCGCTTTCAGAGATAAAAGAAGTTTTGATGGCATCTTTACAGGATAAAACTGAAGAATCTTCCAAGCTTTCTCTTGAGCTTAAAAGTTTGCAAGGGAGTTTGGTTTCTTTGCATGATGGGGTTCAAAAGGAGAGAAATCTCAGGGATAAGTTAGAGAGTACAATTACAGATCTCACTTCCCAATTGAATGAAAAGCATTGCCAGTTGCTAGGTTTTGATCAGCAGAAGGCTGAGCTTGTCCATCTAAAAAAATTGGTATCGGATCTAGAACTTGAGAAATCAAGAGTTTCCTGTCTCTTGTTGGAATCTGAGGAATGTTTAAAAGACGTCCGTGAAGAGTGTTCCTCCATTTCTGCCTTGGAGGCTCAATTGTCTGAAATGCATGAATTTTCAATAGCTGCTGATGTTGGACTCACTTTCACCAAAGTTCAATATGAGACAAGGATTGAAGAACTAGAAAGGTATAACCCGACTGTAGCAGAATTATCAGAGGAGAAAGAAGCTTTAATGGAGTCCTTGCAGAATAAAACTGAAGAATCTTTCAAGCTTTGTCTGGAGCTTAATAGTATGCAAGGAAGTTTGCTCTCTTTGCAAGATGAGTTGCAAACTGAGAGAAATCTCAGAGATAAGTCAGAGAGTAGAATTACAGATCTGACTTCCCAATTGAATGAGAAGAACAGTCAGTTGCTAGATTTCAATCAACAGATGGCTGAGCTGGTCCATCTCAAACTATTGGTATCGGAGCTAGAGCTAGAAAAATCAAGAGTTCTCCGTCTCCTGTTGGATTCTGAGAAGTGTCTTAAAGATGCTCGTGAAGAGTGTTCTTCAGTCTCTGCTTTGGAAGCTCAGTTATCTGAAATGCATGAATTTTCAATAGCTGCTGATGTTGGACTCACTTTCACAAAAACTCAATATGAGGTCGTGATCGAAGAGCTGTGTCAGAAACTGCACTTTTCAGATAGCCAAGTTTCTGATATTCGCAACAATTTCCTTAATGTAGATAATATGCTTAACAAATGTCTTGCTAGCGAAGGGCATTACCTTGAAGAGAACACTCACTTGATGACAAGTCTCAATTCCCTTAAATCTGAGTTGGACGCCTCCAGTGCTCAAAACAGGATACTTCTTGATGCAAACAGTGCAATGAGGACTGAACTTGAGGAATACAATAAAAGAGCTGATAATACGGAGGATATTGTCCGCATGGATAAAAATCAGTCTGCTCTTGAGGTTGAAAGGCTGGAACATCTTCTAATGACATCTGAAGAAGAGATCAATAACCTGATATTCTCCAAGGAAGAACTAGAAGTCAAAGCTTTAGTACTCAAAGCCAAGTTGGATGAACAGTCCGCTCAGATAACCTTGCTGGAAGGATGTAAGGATGAAATGGAAATGCTGCACGATAGATGTAGAGAGCTTACACAGAAGGTTGCTGAACAGGTCTTAAAGACGGAAGAGTTTAAAAACTTGTCCATCCATTTTAAGGATCTTAAAGACAAGGCTTATGCCGAGGGACTCCATGCACAGGATAAAAAAGAACCAGGAGGGCCACCAGCTGCCATGCCAGAGTCCTTAAGAATTGTATTCATCAAAGAACAATATGAAACCAAGCTGCAAGAACTGAAACAACTGCTTGCTATGTCCAATAAGCATAGTGAGGAGATGCTCCGGAAATTACAAGATGCAGTCAATGAAGTTGAGAACAAGAAGAAATCAGAAGCTACTAATGTGAAGAGAAATGAAGAGCTAGGAATGAGGATATTGGAATTGGAGTCTGACCTACATTCAGTGCTTTCTGAAAAGCGTGAAATAATGAAAGCATATGACCTGATGAAAGCTGAAAAGGAATGCTCATTAATAAGCCTCGAGTGCTGCAAGGCAGAAAAACAAGAGCTTGAAGCATCTTTGCAGAAGTGCAACGAGGAAAAAGCTAAAATTGCTTTAGAACTCACCTCAGCAAAGGACTTGCTTGAGAGCACGTCATCATCTATAAATTATCAGAGGGACGCAGACACATCTGGCCTGCAAAGCAGCAGAATAGCAGAGGAAACTTTAGCTAAGTTTTCTGAGCTAGATGTAGCCAATGGTGAAGCATCTCAGCGTGAATGCATGAATTCCATTGATGAAGCAGACCAGTCAAATGTACTCAATAATATAAACTCCAAGCAGGATGATCTTGTATCCAGAGGTGTAAATGGGATCTCTAGTATTGTACTTTCAAAGCAAAGGGATACTCTTAATACCGACATGAAGCATTTGGTTCTTGCCAACGAGAATTTTAAGGCCCAAAGCTTAAAGTCTAGCATGGAAAACTTAGATAAGGAGTTGGAAAGGATGAAACATGAAAATTTGCTTCTTCCAATCGATGATCAACACCTTGACCCAAATTTTCCTGGTTTACAAAGAGAGATTATGCAATTAAAGAAGGCAAATGAAGAATTGGGAAATATATTTCCCTCATTCAATGAATTTTCTTGCAGTGGCAATGCATTAGAGAGGGTGCTAGCTTTGGAAATTGAACTTGCTGAAGCATTGCGGGCAAAGAAGAAATCAATTATCCAGTTTCAAAGTTCTTTCGTGAAACAACACAGTGATGAGGAAGCAGTGTTTCACAGCTTTAGAGACATCAACGAGCTAATTAAAGACATGCTGGAGATTAAGGGAAGATATACTACCGTGGAAACAGAACTCAAAGACATGCATGACCGTTACTCCCAGTTAAGCCTTCAATTTGCAGAGGTCGAAGGGGAGAGACAAAAACTAATGATGACTCTCAAGAATGTCCGAGCGTCAAAGAAAGCCCTATACTTGAATTGCTCCTCAACATCCCCTTTTCTAGACCCTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

2202

Amino Acids

250.56

Weight (kDa)

4.93

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 141 4.1e-26 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 4086
Acc36I ACCTGC 1 cut(s) 130
AccB7I CCANNNNNTGG 2 cut(s) 846, 2485
AccBSI CCGCTC 1 cut(s) 5108
AccI GTMKAC 1 cut(s) 478
AccII CGCG 1 cut(s) 2991
AccIII TCCGGA 2 cut(s) 131, 5424
AciI CCGC 9 cut(s) 747, 1162, 1490, 2455, 2686, 2832, 4957, 5106, 6292
AclWI GGATC 9 cut(s) 188, 1086, 2172, 2446, 2751, 2845, 4026, 5250, 5963
AcoI YGGCCR 1 cut(s) 3297
AcyI GRCGYC 2 cut(s) 4083, 4854
AdeI CACNNNGTG 1 cut(s) 18
AfeI AGCGCT 1 cut(s) 3759
AflII CTTAAG 2 cut(s) 962, 5329
AflIII ACRYGT 2 cut(s) 400, 708
AjiI CACGTC 2 cut(s) 711, 5736
AjuI GAANNNNNNNTTGG 6 cut(s) 1262, 1294, 2237, 2269, 2468, 2500
AloI GAACNNNNNNTCC 2 cut(s) 1289, 1321
Alw21I GWGCWC 5 cut(s) 465, 980, 3210, 4867, 5735
AlwI GGATC 9 cut(s) 188, 1086, 2172, 2446, 2751, 2845, 4026, 5250, 5963
AlwNI CAGNNNCTG 4 cut(s) 884, 3002, 4589, 4702
Ama87I CYCGRG 2 cut(s) 3125, 5621
Aor13HI TCCGGA 2 cut(s) 131, 5424
Aor51HI AGCGCT 1 cut(s) 3759
AoxI GGCC 9 cut(s) 622, 1513, 2595, 3108, 3235, 3297, 5303, 5776, 6039
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
AseI ATTAAT 1 cut(s) 5612
Asp700I GAANNNNTTC 2 cut(s) 2898, 5004
AspLEI GCGC 3 cut(s) 2993, 3079, 3760
AspS9I GGNCC 9 cut(s) 177, 623, 1939, 3235, 3427, 3544, 4474, 5303, 6040
AsuHPI GGTGA 8 cut(s) 767, 985, 1445, 2254, 3739, 4168, 5698, 5855
AsuII TTCGAA 1 cut(s) 578
AsuNHI GCTAGC 2 cut(s) 4781, 6259
AvaI CYCGRG 2 cut(s) 3125, 5621
AvaII GGWCC 5 cut(s) 177, 1939, 3427, 3544, 4474
AxyI CCTNAGG 1 cut(s) 1214
BalI TGGCCA 1 cut(s) 3299
BanII GRGCYC 5 cut(s) 465, 853, 980, 2202, 3210
BauI CACGAG 1 cut(s) 4566
BbsI GAAGAC 3 cut(s) 38, 2516, 3153
Bbv12I GWGCWC 5 cut(s) 465, 980, 3210, 4867, 5735
BbvCI CCTCAGC 3 cut(s) 2387, 3243, 5710
BcgI CGANNNNNNTGC 2 cut(s) 3221, 3255
BciVI GTATCC 6 cut(s) 308, 523, 3574, 4869, 5947, 5980
BclI TGATCA 5 cut(s) 556, 814, 3523, 3973, 6124
BfmI CTRYAG 7 cut(s) 876, 1539, 1872, 2393, 2747, 2768, 4230
BfoI RGCGCY 1 cut(s) 3761
BfrI CTTAAG 2 cut(s) 962, 5329
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 6 cut(s) 308, 523, 3574, 4869, 5947, 5980
BglI GCCNNNNNGGC 1 cut(s) 4118
BglII AGATCT 4 cut(s) 1117, 3475, 3925, 4405
BlpI GCTNAGC 3 cut(s) 3537, 3987, 4467
BmcAI AGTACT 2 cut(s) 1446, 5079
Bme18I GGWCC 5 cut(s) 177, 1939, 3427, 3544, 4474
BmeT110I CYCGRG 2 cut(s) 3125, 5621
BmgBI CACGTC 2 cut(s) 711, 5736
BmgT120I GGNCC 9 cut(s) 177, 623, 1939, 3235, 3427, 3544, 4474, 5303, 6040
BmiI GGNNCC 3 cut(s) 178, 838, 2826
BmrI ACTGGG 2 cut(s) 1374, 6466
BmtI GCTAGC 2 cut(s) 4785, 6263
BmuI ACTGGG 2 cut(s) 1374, 6466
BoxI GACNNNNGTC 1 cut(s) 38
BpiI GAAGAC 3 cut(s) 38, 2516, 3153
BplI GAGNNNNNCTC 6 cut(s) 962, 994, 3119, 3151, 4085, 4117
BpmI CTGGAG 8 cut(s) 1077, 2372, 3077, 3194, 3599, 4331, 4843, 6428
Bpu10I CCTNAGC 3 cut(s) 2387, 3243, 5710
Bpu1102I GCTNAGC 3 cut(s) 3537, 3987, 4467
Bpu14I TTCGAA 1 cut(s) 578
Bsa29I ATCGAT 1 cut(s) 6120
BsaBI GATNNNNATC 2 cut(s) 4452, 4968
BsaHI GRCGYC 2 cut(s) 4083, 4854
BsaI GGTCTC 1 cut(s) 3707
BsaJI CCNNGG 9 cut(s) 156, 276, 301, 373, 2027, 4113, 5049, 5265, 6432
BsaWI WCCGGW 3 cut(s) 89, 131, 5424
BsaXI ACNNNNNCTCC 4 cut(s) 354, 384, 6452, 6482
Bse21I CCTNAGG 1 cut(s) 1214
Bse8I GATNNNNATC 2 cut(s) 4452, 4968
BseAI TCCGGA 2 cut(s) 131, 5424
BseCI ATCGAT 1 cut(s) 6120
BseDI CCNNGG 9 cut(s) 156, 276, 301, 373, 2027, 4113, 5049, 5265, 6432
BseJI GATNNNNATC 2 cut(s) 4452, 4968
BseRI GAGGAG 5 cut(s) 1139, 2174, 4262, 5429, 6565
BseYI CCCAGC 1 cut(s) 858
BsgI GTGCAG 5 cut(s) 431, 2075, 2584, 4688, 5138
Bsh1236I CGCG 1 cut(s) 2991
BshFI GGCC 9 cut(s) 624, 1515, 2597, 3110, 3237, 3299, 5305, 5778, 6041
BshVI ATCGAT 1 cut(s) 6120
BsiHKAI GWGCWC 5 cut(s) 465, 980, 3210, 4867, 5735
BsiHKCI CYCGRG 2 cut(s) 3125, 5621
BsiSI CCGG 3 cut(s) 90, 132, 5425
BslFI GGGAC 4 cut(s) 190, 757, 5283, 5774
BsmBI CGTCTC 1 cut(s) 4535
BsmFI GGGAC 4 cut(s) 190, 757, 5283, 5774
BsmI GAATGC 5 cut(s) 1652, 1729, 2334, 5609, 5867
BsnI GGCC 9 cut(s) 624, 1515, 2597, 3110, 3237, 3299, 5305, 5778, 6041
Bso31I GGTCTC 1 cut(s) 3707
BsoBI CYCGRG 2 cut(s) 3125, 5621
Bsp119I TTCGAA 1 cut(s) 578
Bsp1286I GDGCHC 7 cut(s) 465, 853, 980, 2202, 3210, 4867, 5735
Bsp13I TCCGGA 2 cut(s) 131, 5424
Bsp1407I TGTACA 1 cut(s) 1743
Bsp1720I GCTNAGC 3 cut(s) 3537, 3987, 4467
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 9 cut(s) 747, 1162, 1490, 2455, 2686, 2832, 4957, 5106, 6292
BspANI GGCC 9 cut(s) 624, 1515, 2597, 3110, 3237, 3299, 5305, 5778, 6041
BspDI ATCGAT 1 cut(s) 6120
BspEI TCCGGA 2 cut(s) 131, 5424
BspFNI CGCG 1 cut(s) 2991
BspHI TCATGA 2 cut(s) 1201, 1669
BspLI GGNNCC 3 cut(s) 178, 838, 2826
BspMAI CTGCAG 3 cut(s) 880, 2397, 2751
BspMI ACCTGC 1 cut(s) 130
BspOI GCTAGC 2 cut(s) 4785, 6263
BspPI GGATC 9 cut(s) 188, 1086, 2172, 2446, 2751, 2845, 4026, 5250, 5963
BspQI GCTCTTC 2 cut(s) 4680, 5493
BspT104I TTCGAA 1 cut(s) 578
BspTI CTTAAG 2 cut(s) 962, 5329
BspTNI GGTCTC 1 cut(s) 3707
BsrBI CCGCTC 1 cut(s) 5108
BsrGI TGTACA 1 cut(s) 1743
BssECI CCNNGG 9 cut(s) 156, 276, 301, 373, 2027, 4113, 5049, 5265, 6432
BssNI GRCGYC 2 cut(s) 4083, 4854
BssSI CACGAG 1 cut(s) 4566
BssT1I CCWWGG 7 cut(s) 156, 276, 301, 373, 2027, 4113, 5049
Bst2BI CACGAG 1 cut(s) 4566
BstACI GRCGYC 2 cut(s) 4083, 4854
BstAFI CTTAAG 2 cut(s) 962, 5329
BstAPI GCANNNNNTGC 1 cut(s) 4895
BstAUI TGTACA 1 cut(s) 1743
BstBI TTCGAA 1 cut(s) 578
BstDSI CCRYGG 2 cut(s) 276, 6432
BstEII GGTNACC 1 cut(s) 271
BstENI CCTNNNNNAGG 2 cut(s) 611, 3242
BstFNI CGCG 1 cut(s) 2991
BstH2I RGCGCY 1 cut(s) 3761
BstHHI GCGC 3 cut(s) 2993, 3079, 3760
BstNSI RCATGY 5 cut(s) 404, 1744, 1902, 6406, 6457
BstPAI GACNNNNGTC 1 cut(s) 38
BstPI GGTNACC 1 cut(s) 271
BstSFI CTRYAG 7 cut(s) 876, 1539, 1872, 2393, 2747, 2768, 4230
BstUI CGCG 1 cut(s) 2991
BstV2I GAAGAC 3 cut(s) 38, 2516, 3153
Bsu15I ATCGAT 1 cut(s) 6120
Bsu36I CCTNAGG 1 cut(s) 1214
BsuI GTATCC 6 cut(s) 308, 523, 3574, 4869, 5947, 5980
BsuRI GGCC 9 cut(s) 624, 1515, 2597, 3110, 3237, 3299, 5305, 5778, 6041
BsuTUI ATCGAT 1 cut(s) 6120
BtgI CCRYGG 2 cut(s) 276, 6432
BtrI CACGTC 2 cut(s) 711, 5736
BtsI GCAGTG 4 cut(s) 1926, 2111, 6241, 6366
BveI ACCTGC 1 cut(s) 130
CaiI CAGNNNCTG 4 cut(s) 884, 3002, 4589, 4702
CciI TCATGA 2 cut(s) 1201, 1669
CfoI GCGC 3 cut(s) 2993, 3079, 3760
Cfr13I GGNCC 9 cut(s) 177, 623, 1939, 3235, 3427, 3544, 4474, 5303, 6040
ClaI ATCGAT 1 cut(s) 6120
CseI GACGC 4 cut(s) 1022, 4862, 5771, 6534
DraI TTTAAA 2 cut(s) 4077, 5218
DraIII CACNNNGTG 1 cut(s) 18
EaeI YGGCCR 1 cut(s) 3297
Ecl136II GAGCTC 3 cut(s) 463, 978, 3208
Eco130I CCWWGG 7 cut(s) 156, 276, 301, 373, 2027, 4113, 5049
Eco147I AGGCCT 1 cut(s) 2597
Eco24I GRGCYC 5 cut(s) 465, 853, 980, 2202, 3210
Eco31I GGTCTC 1 cut(s) 3707
Eco32I GATATC 1 cut(s) 531
Eco47I GGWCC 5 cut(s) 177, 1939, 3427, 3544, 4474
Eco47III AGCGCT 1 cut(s) 3759
Eco53kI GAGCTC 3 cut(s) 463, 978, 3208
Eco81I CCTNAGG 1 cut(s) 1214
Eco88I CYCGRG 2 cut(s) 3125, 5621
Eco91I GGTNACC 1 cut(s) 271
EcoICRI GAGCTC 3 cut(s) 463, 978, 3208
EcoNI CCTNNNNNAGG 2 cut(s) 611, 3242
EcoO109I RGGNCCY 3 cut(s) 177, 1939, 3235
EcoO65I GGTNACC 1 cut(s) 271
EcoRI GAATTC 2 cut(s) 574, 5869
EcoRV GATATC 1 cut(s) 531
EcoT14I CCWWGG 7 cut(s) 156, 276, 301, 373, 2027, 4113, 5049
EcoT38I GRGCYC 5 cut(s) 465, 853, 980, 2202, 3210
ErhI CCWWGG 7 cut(s) 156, 276, 301, 373, 2027, 4113, 5049
Esp3I CGTCTC 1 cut(s) 4535
FaqI GGGAC 4 cut(s) 190, 757, 5283, 5774
FauI CCCGC 3 cut(s) 1169, 2679, 6285
FauNDI CATATG 1 cut(s) 5578
FbaI TGATCA 5 cut(s) 556, 814, 3523, 3973, 6124
FblI GTMKAC 1 cut(s) 478
FriOI GRGCYC 5 cut(s) 465, 853, 980, 2202, 3210
GlaI GCGC 3 cut(s) 2992, 3078, 3759
GsaI CCCAGC 1 cut(s) 862
GsuI CTGGAG 8 cut(s) 1077, 2372, 3077, 3194, 3599, 4331, 4843, 6428
HaeII RGCGCY 1 cut(s) 3761
HaeIII GGCC 9 cut(s) 624, 1515, 2597, 3110, 3237, 3299, 5305, 5778, 6041
HapII CCGG 3 cut(s) 90, 132, 5425
HgaI GACGC 4 cut(s) 1022, 4862, 5771, 6534
HhaI GCGC 3 cut(s) 2993, 3079, 3760
Hin1I GRCGYC 2 cut(s) 4083, 4854
Hin6I GCGC 3 cut(s) 2991, 3077, 3758
HinP1I GCGC 3 cut(s) 2991, 3077, 3758
HpaII CCGG 3 cut(s) 90, 132, 5425
HphI GGTGA 8 cut(s) 767, 985, 1445, 2254, 3739, 4168, 5698, 5855
HpyCH4IV ACGT 3 cut(s) 710, 4083, 5735
HpySE526I ACGT 3 cut(s) 710, 4083, 5735
Hsp92I GRCGYC 2 cut(s) 4083, 4854
HspAI GCGC 3 cut(s) 2991, 3077, 3758
Kpn2I TCCGGA 2 cut(s) 131, 5424
Ksp22I TGATCA 5 cut(s) 556, 814, 3523, 3973, 6124
LguI GCTCTTC 2 cut(s) 4680, 5493
MaeII ACGT 3 cut(s) 710, 4083, 5735
MbiI CCGCTC 1 cut(s) 5108
MfeI CAATTG 5 cut(s) 3488, 3644, 3938, 4124, 4418
MhlI GDGCHC 7 cut(s) 465, 853, 980, 2202, 3210, 4867, 5735
MlsI TGGCCA 1 cut(s) 3299
MluNI TGGCCA 1 cut(s) 3299
Mox20I TGGCCA 1 cut(s) 3299
MroI TCCGGA 2 cut(s) 131, 5424
MroXI GAANNNNTTC 2 cut(s) 2898, 5004
MscI TGGCCA 1 cut(s) 3299
MslI CAYNNNNRTG 3 cut(s) 996, 1431, 6348
Msp20I TGGCCA 1 cut(s) 3299
MspA1I CMGCKG 4 cut(s) 415, 2350, 2620, 5312
MspCI CTTAAG 2 cut(s) 962, 5329
MspI CCGG 3 cut(s) 90, 132, 5425
MunI CAATTG 5 cut(s) 3488, 3644, 3938, 4124, 4418
Mva1269I GAATGC 5 cut(s) 1652, 1729, 2334, 5609, 5867
MvnI CGCG 1 cut(s) 2991
NcoI CCATGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 5578
NheI GCTAGC 2 cut(s) 4781, 6259
NlaIV GGNNCC 3 cut(s) 178, 838, 2826
NmeAIII GCCGAG 1 cut(s) 5290
NmuCI GTSAC 3 cut(s) 10, 403, 1310
NspI RCATGY 5 cut(s) 404, 1744, 1902, 6406, 6457
NspV TTCGAA 1 cut(s) 578
PaeI GCATGC 1 cut(s) 1902
PaeR7I CTCGAG 1 cut(s) 5621
PagI TCATGA 2 cut(s) 1201, 1669
PceI AGGCCT 1 cut(s) 2597
PciI ACATGT 1 cut(s) 400
PciSI GCTCTTC 2 cut(s) 4680, 5493
PctI GAATGC 5 cut(s) 1652, 1729, 2334, 5609, 5867
PdmI GAANNNNTTC 2 cut(s) 2898, 5004
PflMI CCANNNNNTGG 2 cut(s) 846, 2485
PfoI TCCNGGA 1 cut(s) 787
PpuMI RGGWCCY 2 cut(s) 177, 1939
PscI ACATGT 1 cut(s) 400
PshAI GACNNNNGTC 1 cut(s) 38
PshBI ATTAAT 1 cut(s) 5612
Psp124BI GAGCTC 3 cut(s) 465, 980, 3210
Psp5II RGGWCCY 2 cut(s) 177, 1939
PspEI GGTNACC 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 858
PspN4I GGNNCC 3 cut(s) 178, 838, 2826
PspPI GGNCC 9 cut(s) 177, 623, 1939, 3235, 3427, 3544, 4474, 5303, 6040
PspPPI RGGWCCY 2 cut(s) 177, 1939
PspXI VCTCGAGB 1 cut(s) 5621
PsrI GAACNNNNNNTAC 2 cut(s) 3024, 3056
PstI CTGCAG 3 cut(s) 880, 2397, 2751
PstNI CAGNNNCTG 4 cut(s) 884, 3002, 4589, 4702
PvuII CAGCTG 4 cut(s) 415, 2350, 2620, 5312
RseI CAYNNNNRTG 3 cut(s) 996, 1431, 6348
SacI GAGCTC 3 cut(s) 465, 980, 3210
SapI GCTCTTC 2 cut(s) 4680, 5493
Sau96I GGNCC 9 cut(s) 177, 623, 1939, 3235, 3427, 3544, 4474, 5303, 6040
ScaI AGTACT 2 cut(s) 1446, 5079
SduI GDGCHC 7 cut(s) 465, 853, 980, 2202, 3210, 4867, 5735
SfcI CTRYAG 7 cut(s) 876, 1539, 1872, 2393, 2747, 2768, 4230
Sfr274I CTCGAG 1 cut(s) 5621
SfuI TTCGAA 1 cut(s) 578
SinI GGWCC 5 cut(s) 177, 1939, 3427, 3544, 4474
SlaI CTCGAG 1 cut(s) 5621
SmiMI CAYNNNNRTG 3 cut(s) 996, 1431, 6348
SphI GCATGC 1 cut(s) 1902
SseBI AGGCCT 1 cut(s) 2597
SsiI CCGC 9 cut(s) 747, 1162, 1490, 2455, 2686, 2832, 4957, 5106, 6292
SspI AATATT 3 cut(s) 1812, 2099, 2755
SstI GAGCTC 3 cut(s) 465, 980, 3210
StuI AGGCCT 1 cut(s) 2597
StyI CCWWGG 7 cut(s) 156, 276, 301, 373, 2027, 4113, 5049
TaiI ACGT 3 cut(s) 713, 4086, 5738
TaqI TCGA 9 cut(s) 578, 1323, 1612, 2450, 3668, 4683, 5622, 6120, 6498
TatI WGTACW 7 cut(s) 1444, 1743, 2878, 3914, 5077, 5899, 5968
TseFI GTSAC 3 cut(s) 10, 403, 1310
Tsp45I GTSAC 3 cut(s) 10, 403, 1310
TspGWI ACGGA 5 cut(s) 1072, 4076, 4517, 4958, 5222
Van91I CCANNNNNTGG 2 cut(s) 846, 2485
Vha464I CTTAAG 2 cut(s) 962, 5329
VpaK11BI GGWCC 5 cut(s) 177, 1939, 3427, 3544, 4474
VspI ATTAAT 1 cut(s) 5612
XagI CCTNNNNNAGG 2 cut(s) 611, 3242
XbaI TCTAGA 3 cut(s) 3571, 4021, 6592
XceI RCATGY 5 cut(s) 404, 1744, 1902, 6406, 6457
XcmI CCANNNNNNNNNTGG 2 cut(s) 90, 4111
XhoI CTCGAG 1 cut(s) 5621
XmiI GTMKAC 1 cut(s) 478
XmnI GAANNNNTTC 2 cut(s) 2898, 5004
ZraI GACGTC 1 cut(s) 4084
ZrmI AGTACT 2 cut(s) 1446, 5079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.