Rw7G040860

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003440
Physical Location & Seq
Reverse (-)
63739521 .. 63747773
8253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G040860.1

Sequence Viewer

Length: 5685 bp
ATGTCGAGGATCACTAAGTGGAAGCTTGAGAAGACGAAAGTGAAAGTGGTTTTCAGGCTACAATTCAACGCTACACATATTCCCCAAAGCGGATGGGATAAGTTGTTTATATCTTTCATACCTGCTGATTCTGGAAAGGCAACTGCAAAGACAACCAAAGCAAATGTGAGGAATGGAACATGCAAATGGGCAGATCCTATCTACGAAACTACAAGACTTCTACAAGACACTAAAACAAAGAAATTTGATGAGAAGCTCTATAAACTTGTGGTGACCATGGGCTCTTCACGGTCTAGTGTCCTTGGGGAGACCAATATCAACCTTGCTGATTATGCCGACGCGTCAAAGCCTTCTGCTGTTGCACTGCCTCTACATGGATGTGATTCTGGAACTATTTTACATGTAACTGTACAGCTATTAACTTCCAAAACTGGTTTCAGAGAGTTTGAACAGCAGAGGGAGCTCAGAGAGAGTGGTTTATGTACAACGTCAGACCAAAGCAGAAATGATGTGTCCACTGCTAAAAGAATATCGTCTTCCGAAGATACAATCAATGCGAGAGTTAGGTTCAAAGAAGAACTCTCTCCCCTTGAAGACGAGGTTGGGCGGAATGAAGAGTTTCCAGACTCAACTGTAGGGTTTGATGGTTCTTCCAATACTTCAGAAAGTTTATACACTGAGAAACATGATACCTCCAGCACCCATGAGATTGACAGCCTTAAGAGTACAACCTCTGGTGATTTAGGTGGACTGTCCCTTGGTCAAAGTCCTCGAAAGGAGAAAGGAGACCACTCTGATCAACGTTTCTTGGTACAGGGGACCAGTGAGTGGGCTCATAGTTGGGCTTCTGACTATTCTGGAGATGCCGACTTGCCAAATGCTTATGAGGAGAATAGTAGACTTAGAGGAAGCTTGGAAGCAGCTGAGTCATCCATTCTTGAGCTTAGGCAGGAAGTAAGCTCATTACAGAGCCAAGCTGATGAAATAGGAGTTGAAGCCCAAAAATTTTCCCTTCAACTTGATGCAGAAATTTGTTCAAGTGAACAGTTAGCCAAAGAAGTTTCTATACTGAGATCAGAATGTTCAAAATTGAAAGAAGATCTTGAAGTGCAGAAAAACTCCAAATTAAGAATTCCATTCACCAGCACACAAACTATTGAGACAGGTCAGGAGGACTTTTTGCAGGAGTTACAGCTCAGGTGGTTAAAGGGGCTTGGGGATGTGGAGGATAAAATTAAAGAGCTTCAAAGCAAAGCAGCCGTTGGAGTACATGAGAGGGACTTCAGGTCCTTTCATTCAGATTTAGAGGCATTGCTTGGTGTTTTGCAAGTTCTAAAGCCAGTAACTGGACAACCGATTTTGGGGATGAACAAGGCAAGCATAAAGGAGACAAATGAAACGAGTGTACATAAAGATGAGCAATTAGTACTAGGAACTAGGTTTGAAGCAGATTTCTATCCTGAAGGTATGCTTCATGGTCTAAGTATGCCTGGCTTGGTGTCACAAGAGTTTGATTCTTTAGATGCTTCAAATGCAATGAAAAGCAAATTCTTTGAGCTTTTAAGAGAGTTGGATGAATTAAAAGCTGAGCGAGAGAGCCTTGCCAAAAAAGCAGACCAGATGGAGTGCTACTATGAAGCTCTCATTCATGAATTAGAAGAAAATCAGAGACAGATGATGGGGGAGTTGCAGAGTCTCAGAAATGAGCATTCTACTTGCCTGTACACAATTTCATCTGCTAAAGCTGAGATGGGGAGAATCCAGCTAGATATGAACAATGAGATAACAAAATTTTCCAAAGAAAGGCATGATTTGGAATCTCTCACCAAGGAGCTCGAAAGAAGGGCTGCTACAGCAGAAGCAGCACTTAAAAGGGCACGCTTGAATTACTCCATTGCGGTGGACCATTTACAGAAGGACCTTGAATTGCTTTCTTCTCAGGTCCTGTCCATGCATGAGACTAATGAGAACCTCATTAAGCAGCTTTTGCAGATTCTGTGCTTCCAAGCTTTCAGGGGCATTTTTCATTCTGGAAAGCACATGCAGCGTCCAAACCAAAGCAATGGTGTAAAAAGACAACATTTAGACGGGGATATACTATCAGACGATCTGAGAAGATCTCTCCTCTTGCAGAAGGAAACATACCAAAAGGTTGAAGAAGAAGTTTATGAAGTGCATCTGGTGAATGTATATTTGGACATCTTCTCAAAGACCCTAGAGGTCACTTTGATTGAAGCAAGTGCTGACTTTGGATTGGTCAAAGAGCAAGTGCATGAACTTACACACCAGCTGGAGCTTTCAACTGAATCAAAGGAGTTATTGATGCTGAGGCTACAGACTGCCTTGGATGAGATTCGCTGCCTGAATGAAGACAAGTACATTTGGAATTCAAAGTGCAATGACTTGACTCTAAAGAACCAAAATTTGGAAGAAGATTTTCGGACTGCCATGGATGAGATTCGCTGCCTGAATGAAGAAAATAATCTTCATTCTCAGAAGATTGCTGAATGGAAAGATCTTGTAAAAGAATATGAAACCTATGAGAGCAAATATAGAGCCTGCAGTACAGAAAAATTGGAGTTAGCAAATTTATTGGAAAAGGAAGCCCTAGAGAATAAGAATCTTCAAAACAGACTTTCATCTTTGCAGGAAGAGTTGAAAGCTGAGAGTCTACAGAATATTGTAAATTCTTCAGAGGGTAAGTTGTGGAATCTGTTGGCATCATATGATATAAAGTATAAAGGTCTGTCTCTGTCTCTCTGCAGTGAATATAACTCTCAAAATTTGGGATCCAAGGATTTAACTGCTGTGGTGGTGCAAATAGAAGAGCTTCAGCACAATTTATATGAGAAGATTGTCCAGCTTATGGAAGAGAAGAAAGACCTAGCACAAGAAAGAGATACTTTAAGAGCAGCCAATTCTGATAATCTGATCATGAAACAGAAATTTGAACAGGATCTACGGGGTATGATGGATAAACTAGATGTTTCAAATGCCCTGGTGCAAAAGCTTCAGTTACAAGTTGGGGCCATTGCTAACAAACTTCAGATTAGTTCTGAGATTGAAGAGAGATATGCACTACAGCACAAAGAACTGTTGGCTGATCTGGATCAGTTGGAAATGGAGCTGCAGCAAATTTCTTTTAAATACCAGGACCTTGCGGAGGAAATTATGGCGTGTAAACTGACGATAGCAGCATTATCGGAGGAAAAAGAAGCTCTAGTGGTGTCCTTGCAGGATAAAACTGAAGAATCTTTCAAGCTTTCTCTGGAGGTTAATAGTTTGCAAGGAAGTTTGCTGTCTTCACGTGATGAGTTGCACATTGAGAAAAATCACAGAGATAAGTTAGCCAGTACAATTTCAGATCTCACTTCCCAACTGGACCAGAAGCATAGTCAGTCTCTAGATTTTGATCAGCAGAAGGATGAGCTGGTCCACCTCAAACAGTTGTTATCAGAATCAGAGCTAGAGAAATCAAGAGTTTGTGCTCGTGAAGAATGTTCTTCTATATCTGCTCTGCAAGCCCATTTGTCTGAAATGTATGAATTTTCAATAGCTGCTGATGTTGGACTCACTTTCACAAAGACTCAATACGAGACCAAGATTGAAGAGCTTGATCAGAAACTTCACTTTTCTGATAGCTGCCTATCTGAGCTTTGCGACAATCATCTTCATGTAGAAAATATGCTTAACAGATGTCTTGCAAGCGAAAAGCATTTAGTTGAAGAGAACACCAAACTAATGACAAGATTAAATGATGCTGGTGAAGAATGTGCTTTTGTATCTGCTCTGGAAGCTCAGCTGTCTGAAATGCATGAAGTTTCAATAGCTGCCGATGTTGGACTCACCTTCGTAGAAGCTCAGTATGGGGCCAAGATTGAAGAGCTTTGCCACAAACTTCACTCTTCTGATAGTCACCTTTCTGAGCTTTGCAACAATCACCTTCATGTGGAGAATAAGCTTAATGAATGTCTTGCTAGTGAAAGGCATTACATTGAAGAGAACACCAAATTAATGGCAAGTCTCAGCTCCCTTAACTCTGAGTTAGAAGCCTCCATTTCTCAAAACAAGATACTTCTTGATACAAACAGTTCCATGAGGACAGAACTCAAGGAATACAAGAAAAGAGCTGAAAATGCAGCAGCTATTGATCATGTGGATAAAAGTCAGTTTGCTCCTGAGATTGAAAGGCTGGAGCATATTGTGGTGACTTCTAATGAAGAGATTGATAACCTGATATTCTCCAAGGAAGAACTAGAAATCAAATATTTAGTGCTCAAGGCCAAGTTGGATGAACAGTGGACTCAAATAGCCTCTCTGGAAGCATATAAAGATGAATTGACAGTGATGCATAATAATGAATGCAATGATCTTAAACACAGGCTTGCTGAACAGGTTTTGAAGGCAGATGAATTTAAGAACTTGTCCATCCATTTCAAGGAGCTTAAAGACAAGTCTTGTGTTGAGTGTCTCCATGCACCTGACAAAAGAGAACCAGAAGCGCCGCCAGCTCCTATGCAAGAGTCCTTAAGAATCGCATTCATCAAAGAACAATATGAAACCAAGCTGCAAGAACTGAAACAGCAACTTGCTATCTCCAAGAAGCATTGTGAGGAGATGCTGTGGAAATTACAAGATGCAATCAATGAAGTTGATAACAGGAAGAAATCTGAAGCTACTCATGTAAAAAGAAACGAAGAGCTAGGAATGAGGATCTTGGAATTGGAGTCTGAGCTACAATCATTGCTTTCTGAAAAGCGTGAAATAATGAGAGCATATGATCTGATGAAGGCTGAAAAGGAATGTTCATTAATAAGCCTCGACTGCTGTAAAGAAGAAAAACAAGATCTTGAAGCATCTTTGCAGAAGTGCAACGAGGAAAAAGTTCAAATTTCTTTAGAACTCACTTCAGCAAAAGATTTAGAAGGGAACGGAAGATTACACAAAGAAGATGGCATTTCTGATGAAGCAGCTGGACTGGAATACTTGAATTCCATTGATGAACCCGAGAAGGATGATATTGTATCCAGAGGTATAAATGGAATTTCTAGTGTTTTACCTTCGAAACAAATGGACGTGCTTAATAGTGACGTGAAGCATTTGGTTCTTGCCAATGAGCATTTCAGGGCCCAAAGCTTAAGGTCTAGCATGGAGAACTTAAATAAGGAGTTGGAAAGGATGAAACATGAAAATTTGCTTCCCCAAGATGATCATCATTTTGACTCAAATTTTCCTGGTTTACAAAGAGACCTGATGCAATTAAATAAGGTTAATGAAGAATTGGGAAGTATTTTTCCCTTGTTCAACGAGTATTCTTGCAGTGGCAATGCATTAGAGAGAGTACTAGCTTTAGAAATTGAACTTGCAGAAGCATTGCAGGCAAAGAAGAAATCATCCTTCCAGTTTCAAAGTTCTTTCTTGAAACAACATGATGACGAGGAAGCAGTGTTTCACAGCTTTAGAGACATAAATGAGCTGATTAAAGACATGCTGGAGATCAAGGGAAGATATGCTACTGTGGAGACAGAACTTAAAGAAATGCATGACCGTTACTCTCAATTAAGCCTTCAATTTGCAGAGGTTGAAGGAGAGAGGCAGAAACTAATGATGACTCTCAAGAATGTTCGAGCATCAAAGAAGCATAGTTTAAACCGCTCCTCGACAACCACTCTACTGGATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1894

Amino Acids

216.05

Weight (kDa)

5.06

Isoelectric Point (pI)

50.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 141 2.4e-25 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 130
AccB7I CCANNNNNTGG 4 cut(s) 828, 1346, 2425, 2866
AccBSI CCGCTC 1 cut(s) 5655
AccI GTMKAC 2 cut(s) 898, 2671
AccII CGCG 1 cut(s) 341
AciI CCGC 6 cut(s) 90, 607, 1898, 3161, 4505, 5653
AclI AACGTT 1 cut(s) 802
AclWI GGATC 8 cut(s) 17, 188, 2784, 2797, 2964, 3117, 4721, 5672
AcvI CACGTG 1 cut(s) 3308
AdeI CACNNNGTG 1 cut(s) 18
AflII CTTAAG 3 cut(s) 719, 4528, 5137
AflIII ACRYGT 2 cut(s) 339, 400
AhdI GACNNNNNGTC 1 cut(s) 1285
AjiI CACGTC 2 cut(s) 5077, 5092
AjnI CCWGG 4 cut(s) 1489, 2997, 3150, 5233
Alw21I GWGCWC 4 cut(s) 465, 1836, 3490, 4278
AlwI GGATC 8 cut(s) 17, 188, 2784, 2797, 2964, 3117, 4721, 5672
AlwNI CAGNNNCTG 3 cut(s) 1346, 1945, 1996
Ama87I CYCGRG 1 cut(s) 5006
AoxI GGCC 4 cut(s) 3027, 3870, 4281, 5127
ApaI GGGCCC 1 cut(s) 5131
AseI ATTAAT 2 cut(s) 4013, 4811
Asp700I GAANNNNTTC 4 cut(s) 618, 2436, 2829, 4884
AspLEI GCGC 1 cut(s) 4504
AsuII TTCGAA 1 cut(s) 5063
AvaI CYCGRG 1 cut(s) 5006
AvaII GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3154, 3382, 3433
BaeGI GKGCMC 2 cut(s) 1879, 5131
BamHI GGATCC 2 cut(s) 2789, 5677
BanII GRGCYC 5 cut(s) 284, 465, 835, 1836, 5131
BarI GAAGNNNNNNTAC 8 cut(s) 1050, 1082, 1834, 1866, 2126, 2158, 5497, 5529
BauI CACGAG 1 cut(s) 3489
BbrPI CACGTG 1 cut(s) 3308
BbsI GAAGAC 5 cut(s) 38, 528, 600, 2376, 3294
Bbv12I GWGCWC 4 cut(s) 465, 1836, 3490, 4278
BbvCI CCTCAGC 1 cut(s) 2327
BccI CCATC 8 cut(s) 87, 638, 1615, 1672, 1744, 2965, 4436, 4946
BceAI ACGGC 1 cut(s) 1244
BcgI CGANNNNNNTGC 2 cut(s) 3183, 3217
BciT130I CCWGG 4 cut(s) 1491, 2999, 3152, 5235
BciVI GTATCC 1 cut(s) 5035
BclI TGATCA 6 cut(s) 796, 2931, 3412, 3616, 4150, 5209
BfmI CTRYAG 8 cut(s) 633, 1851, 2333, 2560, 2672, 2761, 3080, 3128
BfoI RGCGCY 1 cut(s) 4505
BfrI CTTAAG 3 cut(s) 719, 4528, 5137
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 1 cut(s) 5035
BglII AGATCT 5 cut(s) 1099, 2117, 2515, 3364, 4846
BlpI GCTNAGC 2 cut(s) 1587, 3798
BmcAI AGTACT 2 cut(s) 1428, 5343
Bme1390I CCNGG 4 cut(s) 1491, 2999, 3152, 5235
Bme18I GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3154, 3382, 3433
BmeRI GACNNNNNGTC 1 cut(s) 1285
BmeT110I CYCGRG 1 cut(s) 5006
BmgBI CACGTC 2 cut(s) 5077, 5092
BmiI GGNNCC 6 cut(s) 820, 2791, 3028, 3871, 5129, 5679
BmrFI CCNGG 4 cut(s) 1491, 2999, 3152, 5235
BpiI GAAGAC 5 cut(s) 38, 528, 600, 2376, 3294
BplI GAGNNNNNCTC 2 cut(s) 2104, 2136
BpmI CTGGAG 6 cut(s) 679, 879, 2312, 3290, 4214, 5513
Bpu10I CCTNAGC 3 cut(s) 944, 1196, 2327
Bpu1102I GCTNAGC 2 cut(s) 1587, 3798
Bpu14I TTCGAA 1 cut(s) 5063
BpuEI CTTGAG 5 cut(s) 47, 959, 4094, 4262, 5600
BsaAI YACGTR 1 cut(s) 3308
BsaBI GATNNNNATC 4 cut(s) 1455, 3108, 3411, 5211
BsaI GGTCTC 4 cut(s) 302, 780, 3590, 5241
BsaJI CCNNGG 9 cut(s) 276, 301, 757, 1827, 2343, 2448, 2793, 2997, 4245
BsaXI ACNNNNNCTCC 2 cut(s) 4607, 4637
Bse1I ACTGG 9 cut(s) 436, 822, 1340, 1351, 3351, 3384, 4983, 5401, 5679
Bse8I GATNNNNATC 4 cut(s) 1455, 3108, 3411, 5211
BseBI CCWGG 4 cut(s) 1491, 2999, 3152, 5235
BseDI CCNNGG 9 cut(s) 276, 301, 757, 1827, 2343, 2448, 2793, 2997, 4245
BseJI GATNNNNATC 4 cut(s) 1455, 3108, 3411, 5211
BseNI ACTGG 9 cut(s) 436, 822, 1340, 1351, 3351, 3384, 4983, 5401, 5679
BseRI GAGGAG 4 cut(s) 902, 2114, 4628, 5647
BseSI GKGCMC 2 cut(s) 1879, 5131
BsgI GTGCAG 1 cut(s) 1130
Bsh1236I CGCG 1 cut(s) 341
BshFI GGCC 4 cut(s) 3029, 3872, 4283, 5129
BsiHKAI GWGCWC 4 cut(s) 465, 1836, 3490, 4278
BsiHKCI CYCGRG 1 cut(s) 5006
BslFI GGGAC 3 cut(s) 739, 832, 1292
BsmFI GGGAC 3 cut(s) 739, 832, 1292
BsmI GAATGC 3 cut(s) 1708, 4367, 4538
BsnI GGCC 4 cut(s) 3029, 3872, 4283, 5129
Bso31I GGTCTC 4 cut(s) 302, 780, 3590, 5241
BsoBI CYCGRG 1 cut(s) 5006
Bsp119I TTCGAA 1 cut(s) 5063
Bsp120I GGGCCC 1 cut(s) 5127
Bsp1286I GDGCHC 8 cut(s) 284, 465, 835, 1836, 1879, 3490, 4278, 5131
Bsp1407I TGTACA 4 cut(s) 409, 482, 1405, 1722
Bsp1720I GCTNAGC 2 cut(s) 1587, 3798
Bsp19I CCATGG 2 cut(s) 276, 2448
BspACI CCGC 6 cut(s) 90, 607, 1898, 3161, 4505, 5653
BspANI GGCC 4 cut(s) 3029, 3872, 4283, 5129
BspFNI CGCG 1 cut(s) 341
BspHI TCATGA 2 cut(s) 1648, 2934
BspLI GGNNCC 6 cut(s) 820, 2791, 3028, 3871, 5129, 5679
BspMAI CTGCAG 3 cut(s) 2564, 2765, 3132
BspMI ACCTGC 1 cut(s) 130
BspPI GGATC 8 cut(s) 17, 188, 2784, 2797, 2964, 3117, 4721, 5672
BspQI GCTCTTC 5 cut(s) 289, 2820, 3603, 3876, 4692
BspT104I TTCGAA 1 cut(s) 5063
BspTI CTTAAG 3 cut(s) 719, 4528, 5137
BspTNI GGTCTC 4 cut(s) 302, 780, 3590, 5241
BsrBI CCGCTC 1 cut(s) 5655
BsrGI TGTACA 4 cut(s) 409, 482, 1405, 1722
BsrI ACTGG 9 cut(s) 436, 822, 1340, 1351, 3351, 3384, 4983, 5401, 5679
BssECI CCNNGG 9 cut(s) 276, 301, 757, 1827, 2343, 2448, 2793, 2997, 4245
BssSI CACGAG 1 cut(s) 3489
BssT1I CCWWGG 8 cut(s) 276, 301, 757, 1827, 2343, 2448, 2793, 4245
Bst2BI CACGAG 1 cut(s) 3489
Bst2UI CCWGG 4 cut(s) 1491, 2999, 3152, 5235
BstAFI CTTAAG 3 cut(s) 719, 4528, 5137
BstAPI GCANNNNNTGC 1 cut(s) 1987
BstAUI TGTACA 4 cut(s) 409, 482, 1405, 1722
BstBAI YACGTR 1 cut(s) 3308
BstBI TTCGAA 1 cut(s) 5063
BstC8I GCNNGC 8 cut(s) 1378, 1879, 2560, 3522, 3706, 4386, 4509, 5379
BstDSI CCRYGG 2 cut(s) 276, 2448
BstEII GGTNACC 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 3161
BstFNI CGCG 1 cut(s) 341
BstH2I RGCGCY 1 cut(s) 4505
BstHHI GCGC 1 cut(s) 4504
BstNI CCWGG 4 cut(s) 1491, 2999, 3152, 5235
BstNSI RCATGY 4 cut(s) 183, 404, 2044, 5491
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 4 cut(s) 1489, 2997, 3150, 5233
BstSFI CTRYAG 8 cut(s) 633, 1851, 2333, 2560, 2672, 2761, 3080, 3128
BstSLI GKGCMC 2 cut(s) 1879, 5131
BstUI CGCG 1 cut(s) 341
BstV2I GAAGAC 5 cut(s) 38, 528, 600, 2376, 3294
BstXI CCANNNNNNTGG 4 cut(s) 1900, 2063, 4015, 5674
BsuI GTATCC 1 cut(s) 5035
BsuRI GGCC 4 cut(s) 3029, 3872, 4283, 5129
BtgI CCRYGG 2 cut(s) 276, 2448
BtrI CACGTC 2 cut(s) 5077, 5092
BtsI GCAGTG 5 cut(s) 362, 516, 2770, 5326, 5451
BtsIMutI CAGTG 9 cut(s) 362, 516, 675, 829, 2770, 4304, 4350, 5326, 5451
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 8 cut(s) 1378, 1879, 2560, 3522, 3706, 4386, 4509, 5379
CaiI CAGNNNCTG 3 cut(s) 1346, 1945, 1996
CciI TCATGA 2 cut(s) 1648, 2934
CfoI GCGC 1 cut(s) 4504
CseI GACGC 3 cut(s) 330, 347, 2036
DraI TTTAAA 2 cut(s) 3145, 5649
DraIII CACNNNGTG 1 cut(s) 18
DriI GACNNNNNGTC 1 cut(s) 1285
Eam1105I GACNNNNNGTC 1 cut(s) 1285
EciI GGCGGA 1 cut(s) 622
Ecl136II GAGCTC 2 cut(s) 463, 1834
Eco130I CCWWGG 8 cut(s) 276, 301, 757, 1827, 2343, 2448, 2793, 4245
Eco24I GRGCYC 5 cut(s) 284, 465, 835, 1836, 5131
Eco31I GGTCTC 4 cut(s) 302, 780, 3590, 5241
Eco47I GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3154, 3382, 3433
Eco53kI GAGCTC 2 cut(s) 463, 1834
Eco72I CACGTG 1 cut(s) 3308
Eco88I CYCGRG 1 cut(s) 5006
Eco91I GGTNACC 1 cut(s) 271
EcoICRI GAGCTC 2 cut(s) 463, 1834
EcoNI CCTNNNNNAGG 1 cut(s) 3161
EcoO109I RGGNCCY 5 cut(s) 1287, 1918, 1942, 3154, 5127
EcoO65I GGTNACC 1 cut(s) 271
EcoRI GAATTC 3 cut(s) 1131, 2386, 4990
EcoRII CCWGG 4 cut(s) 1489, 2997, 3150, 5233
EcoT14I CCWWGG 8 cut(s) 276, 301, 757, 1827, 2343, 2448, 2793, 4245
EcoT22I ATGCAT 5 cut(s) 1956, 3816, 4353, 5332, 5544
EcoT38I GRGCYC 5 cut(s) 284, 465, 835, 1836, 5131
ErhI CCWWGG 8 cut(s) 276, 301, 757, 1827, 2343, 2448, 2793, 4245
FalI AAGNNNNNCTT 8 cut(s) 1086, 1118, 1455, 1487, 1851, 1883, 2887, 2919
FaqI GGGAC 3 cut(s) 739, 832, 1292
FauNDI CATATG 2 cut(s) 2725, 4777
FbaI TGATCA 6 cut(s) 796, 2931, 3412, 3616, 4150, 5209
FblI GTMKAC 2 cut(s) 898, 2671
FriOI GRGCYC 5 cut(s) 284, 465, 835, 1836, 5131
GlaI GCGC 1 cut(s) 4503
GsuI CTGGAG 6 cut(s) 679, 879, 2312, 3290, 4214, 5513
HaeII RGCGCY 1 cut(s) 4505
HaeIII GGCC 4 cut(s) 3029, 3872, 4283, 5129
HgaI GACGC 3 cut(s) 330, 347, 2036
HhaI GCGC 1 cut(s) 4504
Hin6I GCGC 1 cut(s) 4502
HinP1I GCGC 1 cut(s) 4502
HindIII AAGCTT 7 cut(s) 23, 910, 2007, 3008, 3260, 3959, 5134
Hpy99I CGWCG 1 cut(s) 341
HpyCH4IV ACGT 5 cut(s) 488, 802, 3307, 5076, 5091
HpySE526I ACGT 5 cut(s) 488, 802, 3307, 5076, 5091
HspAI GCGC 1 cut(s) 4502
Ksp22I TGATCA 6 cut(s) 796, 2931, 3412, 3616, 4150, 5209
LguI GCTCTTC 5 cut(s) 289, 2820, 3603, 3876, 4692
MaeII ACGT 5 cut(s) 488, 802, 3307, 5076, 5091
MbiI CCGCTC 1 cut(s) 5655
MhlI GDGCHC 8 cut(s) 284, 465, 835, 1836, 1879, 3490, 4278, 5131
MluI ACGCGT 1 cut(s) 339
MmeI TCCRAC 7 cut(s) 1243, 1551, 3096, 3547, 3820, 4269, 5151
Mph1103I ATGCAT 5 cut(s) 1956, 3816, 4353, 5332, 5544
MroXI GAANNNNTTC 4 cut(s) 618, 2436, 2829, 4884
MslI CAYNNNNRTG 7 cut(s) 184, 378, 1413, 1898, 3672, 3945, 4356
MspA1I CMGCKG 4 cut(s) 923, 2290, 3802, 4973
MspCI CTTAAG 3 cut(s) 719, 4528, 5137
MspR9I CCNGG 4 cut(s) 1491, 2999, 3152, 5235
MssI GTTTAAAC 1 cut(s) 5649
Mva1269I GAATGC 3 cut(s) 1708, 4367, 4538
MvaI CCWGG 4 cut(s) 1491, 2999, 3152, 5235
MvnI CGCG 1 cut(s) 341
NcoI CCATGG 2 cut(s) 276, 2448
NdeI CATATG 2 cut(s) 2725, 4777
NlaIV GGNNCC 6 cut(s) 820, 2791, 3028, 3871, 5129, 5679
NmuCI GTSAC 6 cut(s) 271, 1500, 2221, 3914, 4207, 5087
NsiI ATGCAT 5 cut(s) 1956, 3816, 4353, 5332, 5544
NspI RCATGY 4 cut(s) 183, 404, 2044, 5491
NspV TTCGAA 1 cut(s) 5063
PagI TCATGA 2 cut(s) 1648, 2934
PciI ACATGT 1 cut(s) 400
PciSI GCTCTTC 5 cut(s) 289, 2820, 3603, 3876, 4692
PctI GAATGC 3 cut(s) 1708, 4367, 4538
PdmI GAANNNNTTC 4 cut(s) 618, 2436, 2829, 4884
PflMI CCANNNNNTGG 4 cut(s) 828, 1346, 2425, 2866
PmaCI CACGTG 1 cut(s) 3308
PmeI GTTTAAAC 1 cut(s) 5649
PmlI CACGTG 1 cut(s) 3308
Ppu21I YACGTR 1 cut(s) 3308
PpuMI RGGWCCY 4 cut(s) 1287, 1918, 1942, 3154
PscI ACATGT 1 cut(s) 400
PshBI ATTAAT 2 cut(s) 4013, 4811
Psp124BI GAGCTC 2 cut(s) 465, 1836
Psp1406I AACGTT 1 cut(s) 802
Psp5II RGGWCCY 4 cut(s) 1287, 1918, 1942, 3154
Psp6I CCWGG 4 cut(s) 1489, 2997, 3150, 5233
PspCI CACGTG 1 cut(s) 3308
PspEI GGTNACC 1 cut(s) 271
PspGI CCWGG 4 cut(s) 1489, 2997, 3150, 5233
PspN4I GGNNCC 6 cut(s) 820, 2791, 3028, 3871, 5129, 5679
PspOMI GGGCCC 1 cut(s) 5127
PspPPI RGGWCCY 4 cut(s) 1287, 1918, 1942, 3154
PsrI GAACNNNNNNTAC 2 cut(s) 2943, 2975
PstI CTGCAG 3 cut(s) 2564, 2765, 3132
PstNI CAGNNNCTG 3 cut(s) 1346, 1945, 1996
PvuII CAGCTG 4 cut(s) 923, 2290, 3802, 4973
RseI CAYNNNNRTG 7 cut(s) 184, 378, 1413, 1898, 3672, 3945, 4356
SacI GAGCTC 2 cut(s) 465, 1836
SapI GCTCTTC 5 cut(s) 289, 2820, 3603, 3876, 4692
ScaI AGTACT 2 cut(s) 1428, 5343
ScrFI CCNGG 4 cut(s) 1491, 2999, 3152, 5235
SduI GDGCHC 8 cut(s) 284, 465, 835, 1836, 1879, 3490, 4278, 5131
SfcI CTRYAG 8 cut(s) 633, 1851, 2333, 2560, 2672, 2761, 3080, 3128
SfuI TTCGAA 1 cut(s) 5063
SinI GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3154, 3382, 3433
SmiMI CAYNNNNRTG 7 cut(s) 184, 378, 1413, 1898, 3672, 3945, 4356
SmlI CTYRAG 8 cut(s) 26, 719, 938, 4109, 4277, 4528, 5137, 5615
SmoI CTYRAG 8 cut(s) 26, 719, 938, 4109, 4277, 4528, 5137, 5615
SsiI CCGC 6 cut(s) 90, 607, 1898, 3161, 4505, 5653
SspI AATATT 2 cut(s) 2680, 4268
SstI GAGCTC 2 cut(s) 465, 1836
StyD4I CCNGG 4 cut(s) 1489, 2997, 3150, 5233
StyI CCWWGG 8 cut(s) 276, 301, 757, 1827, 2343, 2448, 2793, 4245
TaiI ACGT 5 cut(s) 491, 805, 3310, 5079, 5094
TaqI TCGA 7 cut(s) 5, 772, 1836, 4821, 5063, 5626, 5660
TauI GCSGC 1 cut(s) 4507
TscAI CASTG 9 cut(s) 369, 523, 682, 829, 2770, 4304, 4350, 5326, 5451
TseFI GTSAC 6 cut(s) 271, 1500, 2221, 3914, 4207, 5087
Tsp45I GTSAC 6 cut(s) 271, 1500, 2221, 3914, 4207, 5087
TspGWI ACGGA 1 cut(s) 4947
TspRI CASTG 9 cut(s) 369, 523, 682, 829, 2770, 4304, 4350, 5326, 5451
Van91I CCANNNNNTGG 4 cut(s) 828, 1346, 2425, 2866
Vha464I CTTAAG 3 cut(s) 719, 4528, 5137
VpaK11BI GGWCC 8 cut(s) 819, 1287, 1903, 1918, 1942, 3154, 3382, 3433
VspI ATTAAT 2 cut(s) 4013, 4811
XagI CCTNNNNNAGG 1 cut(s) 3161
XbaI TCTAGA 1 cut(s) 3403
XceI RCATGY 4 cut(s) 183, 404, 2044, 5491
XmiI GTMKAC 2 cut(s) 898, 2671
XmnI GAANNNNTTC 4 cut(s) 618, 2436, 2829, 4884
ZrmI AGTACT 2 cut(s) 1428, 5343
Zsp2I ATGCAT 5 cut(s) 1956, 3816, 4353, 5332, 5544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.