FvH4_5g38230

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003440
Physical Location & Seq
Reverse (-)
28271960 .. 28282877
10918 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38230.t3

Sequence Viewer

Length: 6522 bp
ATGTCGAGGATCGCTAAATGGAAGCTTGAGAAGACAAAAGTGAAAGTCGTTTTCAGGCTACAATTCAATGCTACACATATTCCCCAAAGCGGATGGGATAAGTTGTTTATATCTTTCATACCTGCTGATTCTGGAAAGGCAACCGCAAAGACAACCAAAGCAAATGTGAGGAATGGAACATGCAAATGGGGAGATCCTATCTACGAAACTACACGACTTCTACAAGACACTAAAACAAAGAAATTTGATGAGAAGCTCTATAAACTTGTGGTGACCATGGGCTCTTCACGGTCCAGTGTCCTTGGGGAAACAAATATCAACCTTGCTGATTATGCCGATGCGTCAAAGCCTTCTTCTGTTGCACTGCCTCTCCATGGATGTGATTTTGGAACAATTTTACATGTAACAGTACAGCTATTAACTTCCAAAACCGGTTTCAGAGAGTTTGAGCAGCAGAGGGAGCTCAGAGAGAGTGGTCTATGTACAACGTCAGACCAAAGCAGAAATGATGTATCCACAGCTAAAAGAATATCGTCTTCTGAAGATACAATCAATGCGAGAGTTAGGTTCAAAGAAGAACTCTCTCCCCATGAAGAGGACATTAGGCAGAGTGAAGAGTATCCTGACTTAACTGTAGGGTTTGATGGTTCTTCCAATACTTCAGAAAGTTTATACGCTGAGAAACATGATACCTCCAGCACCCATGAGATTGACAGCCTTAAGAGTACAACCTCTGGTGATTTAGGTGGACTGTCTGTTGGTCAAAGTCCTCGAAAAGAGAAAGGAGACCCCTCTGATCAACGTTTATCGGCACAGGGGACCAGTGAGTGGGCTCATAGCTGGGCTTCTGACTATTCTGGAGATGCTGACTTGCCAAATGCGTACGAGGAGAACAGTAGACTTAGAGGAAGCCTGGAAGCAGCTGAGTCATCAATTCTTGAGCTTAAGCAGGAAGTAAGCTATTTACAATGCCAAGCTGATGAAATAGGAGTCGAAGCACAAAAGTTTTCCCTTCAGCTTGATGCAGAAATTTCTTCAGGCGAACAATTAGCCAAAGAAGTTTCTATACTGAGATCAGAATGTTCAAAACTGAAAGAAGATCTTGAAGAGCAGAAAAATTCCAAACTAAGAATTCCATACACCAGCAGAGAAACTTTTGCGACAGGCCAGGATGACTTATTGCATGAGTTACAGCTCAGGTGGTTAAAGGGGCTTGGCGATGCAGAGGATAAAATAAGAGAGCTTCAAAGCAAAGCAACCGTCGGGGTACATGAGAGGGACTTCAGGTCCTTTTATTCAGATTTAGAGGCATTGCTTGGTGTTCTGCAAGTTTTAAAGCCAGTAACTGGACAAGCAATTTTGGGGACGAACAAGGCAAGCATAAAGGAGACAAATGAAATGGGTGTACATAAAGATGTGCAATTAGTGCTAGGAACTAGGTTTGATGCAGATTTCTATCCTGAAGGTATGCTTCAGGGTCTAAGTATGCCTGGTGTGGTGTCACAAGAGTTTGATTCTTTAGATGCTGCAAATGCAATGAAAAGTAAATTCTTTGAGCTTTTAAGAGAATTAGATGAATTAAAAGCTGAGCGAGAGAGCCTTGCAAAGAAAGCAGACCAGATGGAGTGTTACTATGAAGCTCTCATTCATGAATTAGAAGAAAATCAGAGGCAGATGATGGGGGAGTTGCAGAGTCTCAGAAATGAGCATTCTACTTGCCTGTATACAATTTCATCTGCTAAAGCTGAGATGGAGAGAATCCAGCTAGATATGAGCAATGAGTTAACAAAATTTTCCAAAGAAAGGCATGATTCGGAGGCCCTCACAAAGGAGCTCGAAAGAAGGGCTGCTACTGCAGAAGCAGCACTTAAAAGGGCACGCTTGAATTATTCCATTGCAGTGGACCATTTACAGAAGGACCTTGAATTGCTTTCTTCTCAGGTCCTATCCATGCATGAGACTAATGAGAATCTCATTAAGCAGGCTTTTGAAGATTCCATGCTTCCAAGCTTTCAGGGGCGTGAGGTGATGATGCAGAATCCTAAACGAGAATCAGGGACTTTTCATGCTGGAAAGCAAATGCAGCATCCAAACCAAAGCAATGGGGTGAAAAGACAACATTTAGACGGGGATATACTATCAAATGATTTGAGAAGATCTCTTCTCTTGCAGAAGGAAACATACCAAAAGGTTGAAGAAGAAGTTTATGAAGTGCATCTGGTGAATGTATATTTGGACATCTTCTCAAAGACCCTAGAGGTCACTTTGATTGAAGCAAGTGCTGACTTTGGATTGGTCAAAGAGAAAGTGCATGAACTTGCACAACAGCTGGAGCTTTCAACTGAGTCGAAGGAGTTATTGATGCTGAGGCTACAGACTGCCTTGGATGAGATTCGCTGCCTGAATGAAGCCAATGATATTTGGACTTCAAAGTGCAGTGACTTGACTCTAAAGAACCAAAATTTGGAAGAAGATTTTCGGACTGCCATGGATGAGATTCGGTGCCTGAATGAGTACAAGGAGACTTGCAATTCAAAGTGCAATGAATTGACTCTAAAGAGCCATAGTTTGGAGGAAGAGGTTCAGAATCTCACAAGAGAAAATAATCTTCATGCCCAGAAGATTGCTGAATGGGAAGACCTTCTAAAAGAATATGAAACCTATGAGAGCAAATATAAAGCCTTCACTATAGAAAAATTGGAGATGGCAAATTTGTTGGAAAGGGAAACCCTAAAGAATAAGAATATTCAAAACGAACTTTCTTCTTTGCAAGAAGAGTTGAAAGCTGTCCAAAATGACTGTGATGAGCTGACATATGTGAAGGAGAGTCTGCAGAATATTGTTATTTCTTCACAGGGTAAGTTGCGGAATCTGTTGGCATCTTATGACATGAAGTATAAAGGTTTGTCTCTTCCTCTCTGCAGTGAATATAACTATCAGGATTTGGAATCCAGGGATTTGACTGGTGTTGTGGTGCAAATAGAAGAGCTTCAGCACAATGTCTATGAGAAGATTGTCCAGCTTATGGAAGAGAAGAATGATCTAGCACAAGAAAAAGATATAGCTCAAATGTCTTTAAGAGCAGCGGATTCTGATAATCTGATCATGAAACAGAAGTTTGAACAGGATCTACGGGGTATGATGGATAAACTAGATGTTTCAAATGCCCTGGTGCATAAGCTTCAGTTAAAAGTTGGGGCCATTGCTAACAAACTTCACATCAGCTCAGAAGTTGAAGAGAGATATGCACAGCAGCACAAAATTCTGTTGACTGATCTGGATCAGTTGGAAATGGAGCTGCAGCAAATTTCTTCAAAATACCAGGACCTTGCGGAGGAAGTTATGGCGTTAGAGACTGTAACTGACGAACTAGGAAGGTGTAAACTGACAATAGCAGCATTATCGGAGGAAAAAGAAGCCCTAGTGGTGTCCTTGCAGGATAAAACTGAAGAATCTTTCAAGCTTTCTCTGGAGGTTAATCGTTTGCAAGGAAGTTTGCTGTCTTCACTTGATGAGTTGCACGTTGAGAAAAATCACAAAGATAAGTTAGCGAGTACAGTTTCAGATCTCACTGCCCAACTGAATGAGAAGCATAGTCAGTTTCTGAATTTTGATCAGCAGAAGGATGAGCTGGTCCACCTCAAACAGTTGTTATCGGAATCAGAGCTAGAGAAATCAAGAGTTTGTGGTCTCTTATTGGAGTCTGAGAAATGTCTAAAAGATGCTCATGAAGAATGTTCTTCTATATCAGGTCTGGAATCCCAGTTGTCTGAATTATACAAACTTTTAATAGCTGCTGATGTTGGACTCATATTTACAAAGACTCAATATGAGACCAAGATTGAAGAGCTTGATCAGAAACTTCACTTTTCTGATAGCTGCCTGTCTGATCTTCATGACAATCACCTTCATGTAGAAAATATGCTTAACAGATGTCTTGCAAGCGAAAGGCATTTAGTTGAAGACAACACCAAACTGATGGCAAGATTAAATGATGCTGGTGAAGAATGTTCTTTGGTATCTTCCCTGGAAGCTCAGCTTTTTGAAATGCATGAAGTTTCATTAGCTGCCGATGTTGGACTCACCTTTGCAGTAGCTCAATATGAGGCCAGGATTGAAGAGCTTGGCCACAAACTTCACTCTTCTGATAGTCACCTTTCTGTGCTCCGCAATAATCAGCTTGAAATGGAGAATAAGCTTAATGAATGTCTTGCTGGTGAAAGGCATTACATTGAAGAGAACACCAAATTAATGACTAGTCTCAGCTCACTTAACTCTGACTTGAAAGCATCCATTGCTCAAAACAGGATACTTCTTGATACAAACAGTTCTGTGGGGATTGAACTTGAGGAATACAAGAAAAGAGGTGAAAATGCAGAAGCTCAATATGAGGCCAGGATTAAAGAGCTTGGCCAGAAACTTGACTCTTCTGATAGTCACCTTTCTGAGATTCGCAAGAATCAGCTTCATGTGGAGAATAAGCTTAATGACTGTATTGCTAGTGAAAAGCACTACATTGAAGAGAACAGCAAATTAATGACAAGTCTCAGCTCCCTTAACTCTGAGTTAGAAGCCTCCATTGGTCAAAACAGGGCACTTCTTGATACAAACAGTTCCTTGAGGACTGAACTTGAGGATTACAAGAAAAGAGCTGAAAATGCAGAAGCTCAATATGAGGCCAGGATTGAAGAGCTTGGCCAGAAACTTGACTCTTCCGATAGTCACCTTTCTGAGATTCGCAACAATCAGCTTCATCTGGAGAATAAGCTTAATGAATGTCTTGCTAGTGAAAAGCACTACATTGAAGAGAACTGCAAATTAATGACAAGTCTCAGCTCCCTTAACTCTGAGTTAGAAGCCTCCATCGGTCAAAACAGGATACTTCTTTATACAAACAGTTCCATGAGGACTGAACTTGAGGAATACAAGAAAAGAGCTGAAAATGCAGTGGCTATTGATCATGGGGATAAAAGTCAGTGCGCTCCTGAGATTGAAAGGCTGGAGCGTATATTGGCGACTTCTGAAGAAGAGGTTGATAACCTGATATTCTCCAAGGAAGAACTAGAAATCAAATATATAGTGATCAAAGCCAAGTTGGATGAACAGTGCACTCAAATAACCTCTCTGGAGGCATATAAAGATGAATCGACACTGATGCATAATGAATGTAATGACCTTAAACAGAAGCTTGCTGAACAGGTTTTGAAGGCAGAGGAATTCAAGAACTTGTCCATCCATTTCAAGGAGCTTAAGGGCAAGTCTTGTGTTGAGTGTCTCCATGCACCTGACAAAAGAGAACCAGAAGCGCCACCAGCTGCTATGCAAGAGTCCTTAAGAATTGCATTCATCAAAGAACAATATGAAACCAAGTTGCAAGAACTGAAACAACAACTTGCTATCTCCAAGAAGCATTGTGAGGAGATGCTGTGGAAATTACAAGATGCAATTAATGAAGTTGATAGCAGGAAGAAATCTGAAGCTACTCATGTAAAGAGAAACGAAGAGCTAGGAATGAGGATCTTGGAATTGGAGTCTGAGATACAATCAGTGCTCTCTGAAAAGCGTGAAATAATGAAAGCATATGATCTGATGAAGGCTGAAAAGGAATGTTCATTAATAAGCCTCGACTGCTGTAAAGAAGAAAAACAAGAGCTTGAAGCATCTTTGCAGAAGTGCAATGAGGAAAAAGTTCAAATTACTTTAGAACTCACTTCAGCAAAAGATTTGCTTCAGAGCTCTTCATCTTATAACCAGAGTGAAGGGAATGAAAAATTGCACAAAGAAGATAGCATTTCTGATGAAGCAGCTGGTCATGAATGCTTGAGTTCCATTGATGAACCAGAGAAGGATGATCTTGTATCCAGAGGTATAAATGGAATTTCTAGTGGTTTACATTTGAAACAAACGGACGTGGTTAATAGTGACAGGAAGCATTTGGTTCTTGCAAATGAGCATTTCAGGGCCCAAAGCTTAAGGTCTAGCATGGAGAACTTAAATAAGGAGTTGGAAAGGATGAAACATGAGAATTTGCTTCCCCTAGATGATCATCATTTTTACTCAAATTTTCCTGGTTTACAAAGAGACCTGATGCAATTAAACAAGGTTAATAAAGAATTAGGAAGCATTTTTCCTTCGTTCAATGAGTATTCTTGCAGTGGCAATGCATTAGAGAGAGTACTAGCTTTGGAAATTGAACTTGCTGAAGCATTGCAGGCAAAGAAGAAATCAACCTTCCAGTTTCAAAGTTCTTTCTTGAAACAACATGATGATGAGGAAGCAGTGTTTCACAGCTTTAGAGACATAAATGAGCTCATCAAAGACATGCTGGAGATCAAGGGAAGATATGCTACTGTGGAGGGGGAACTTAAAGAAATGCATGACCGTTACTCTCAGTTAAGCCTTCAATTTGCAGAGGTTGAAGGAGAGAGGCAGAAACTATTGATGACTCTCAAGAATGTTCGAGCATCAAAGAAAGCATCATACTTTAGCCGCTCCTCAACAACCTCTCTACTGGATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

2174

Amino Acids

247.44

Weight (kDa)

5.03

Isoelectric Point (pI)

49.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 141 1.1e-24 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5748
Acc36I ACCTGC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 2502
AccB7I CCANNNNNTGG 5 cut(s) 828, 1346, 2464, 2569, 3025
AccBSI CCGCTC 1 cut(s) 6492
AccI GTMKAC 2 cut(s) 898, 1724
AciI CCGC 7 cut(s) 90, 144, 2866, 3086, 3332, 4168, 6490
AclI AACGTT 1 cut(s) 802
AclWI GGATC 6 cut(s) 17, 188, 3135, 3288, 5525, 6509
AcoI YGGCCR 3 cut(s) 4126, 4411, 4696
AfaI GTAC 9 cut(s) 411, 484, 727, 884, 1269, 1407, 2516, 3556, 6177
AflII CTTAAG 5 cut(s) 719, 944, 5249, 5332, 5971
AflIII ACRYGT 1 cut(s) 400
AgeI ACCGGT 1 cut(s) 431
AhdI GACNNNNNGTC 1 cut(s) 1285
AhlI ACTAGT 1 cut(s) 4256
AjiI CACGTC 1 cut(s) 5911
AjuI GAANNNNNNNTTGG 8 cut(s) 419, 451, 2216, 2248, 2447, 2479, 4563, 4595
Alw21I GWGCWC 7 cut(s) 465, 1836, 4167, 5111, 5553, 5738, 6312
Alw44I GTGCAC 1 cut(s) 5107
AlwI GGATC 6 cut(s) 17, 188, 3135, 3288, 5525, 6509
AlwNI CAGNNNCTG 2 cut(s) 1346, 3604
ApaI GGGCCC 1 cut(s) 5965
ApaLI GTGCAC 1 cut(s) 5107
ArsI GACNNNNNNTTYG 2 cut(s) 1245, 1277
AseI ATTAAT 5 cut(s) 4250, 4535, 4820, 5448, 5615
AsiGI ACCGGT 1 cut(s) 431
Asp700I GAANNNNTTC 5 cut(s) 2475, 2580, 2640, 2988, 5688
AspLEI GCGC 2 cut(s) 4982, 5308
AvaII GGWCC 8 cut(s) 291, 819, 1287, 1903, 1918, 1942, 3325, 3634
BaeGI GKGCMC 4 cut(s) 1879, 4597, 5111, 5965
BalI TGGCCA 3 cut(s) 4128, 4413, 4698
BamHI GGATCC 1 cut(s) 6514
BanI GGYRCC 1 cut(s) 2502
BanII GRGCYC 7 cut(s) 284, 465, 835, 1836, 5738, 5965, 6312
BarI GAAGNNNNNNTAC 8 cut(s) 1050, 1082, 1834, 1866, 2165, 2197, 6331, 6363
BbsI GAAGAC 5 cut(s) 38, 528, 2643, 3495, 3969
Bbv12I GWGCWC 7 cut(s) 465, 1836, 4167, 5111, 5553, 5738, 6312
BbvCI CCTCAGC 1 cut(s) 2366
BcgI CGANNNNNNTGC 4 cut(s) 3384, 3418, 5137, 5171
BciVI GTATCC 5 cut(s) 523, 630, 4302, 4872, 5869
BclI TGATCA 7 cut(s) 796, 3102, 3613, 3853, 4957, 5082, 6043
BcuI ACTAGT 1 cut(s) 4256
BfmI CTRYAG 7 cut(s) 633, 1854, 2372, 2688, 2831, 2920, 3299
BfoI RGCGCY 1 cut(s) 5309
BfrI CTTAAG 5 cut(s) 719, 944, 5249, 5332, 5971
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 5 cut(s) 523, 630, 4302, 4872, 5869
BglII AGATCT 3 cut(s) 1099, 2156, 3565
BlpI GCTNAGC 2 cut(s) 1587, 4035
BmcAI AGTACT 1 cut(s) 6177
Bme18I GGWCC 8 cut(s) 291, 819, 1287, 1903, 1918, 1942, 3325, 3634
BmeRI GACNNNNNGTC 1 cut(s) 1285
BmgBI CACGTC 1 cut(s) 5911
BmiI GGNNCC 5 cut(s) 820, 2504, 3199, 5963, 6516
BmrI ACTGGG 1 cut(s) 3757
BmuI ACTGGG 1 cut(s) 3757
BpiI GAAGAC 5 cut(s) 38, 528, 2643, 3495, 3969
BplI GAGNNNNNCTC 2 cut(s) 2143, 2175
BpmI CTGGAG 8 cut(s) 679, 879, 2351, 3491, 4778, 5021, 5147, 6347
Bpu10I CCTNAGC 2 cut(s) 1196, 2366
Bpu1102I GCTNAGC 2 cut(s) 1587, 4035
BpuEI CTTGAG 8 cut(s) 47, 959, 4367, 4639, 4652, 4937, 5842, 6434
BsaBI GATNNNNATC 3 cut(s) 1455, 3279, 6045
BsaI GGTCTC 4 cut(s) 780, 3695, 3827, 6075
BsaJI CCNNGG 9 cut(s) 276, 301, 373, 2382, 2487, 2952, 3168, 4026, 5052
BsaWI WCCGGW 1 cut(s) 431
BsaXI ACNNNNNCTCC 4 cut(s) 354, 384, 5411, 5441
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 8 cut(s) 294, 822, 1340, 1351, 2968, 3763, 6235, 6516
Bse8I GATNNNNATC 3 cut(s) 1455, 3279, 6045
BseDI CCNNGG 9 cut(s) 276, 301, 373, 2382, 2487, 2952, 3168, 4026, 5052
BseJI GATNNNNATC 3 cut(s) 1455, 3279, 6045
BseNI ACTGG 8 cut(s) 294, 822, 1340, 1351, 2968, 3763, 6235, 6516
BseRI GAGGAG 3 cut(s) 902, 5432, 6484
BseSI GKGCMC 4 cut(s) 1879, 4597, 5111, 5965
BseYI CCCAGC 1 cut(s) 840
BsgI GTGCAG 1 cut(s) 2455
BshNI GGYRCC 1 cut(s) 2502
BshTI ACCGGT 1 cut(s) 431
BsiHKAI GWGCWC 7 cut(s) 465, 1836, 4167, 5111, 5553, 5738, 6312
BsiSI CCGG 1 cut(s) 432
BsiWI CGTACG 1 cut(s) 882
BslFI GGGAC 4 cut(s) 832, 1292, 1378, 2071
BsmFI GGGAC 4 cut(s) 832, 1292, 1378, 2071
BsmI GAATGC 3 cut(s) 1708, 5342, 5822
Bso31I GGTCTC 4 cut(s) 780, 3695, 3827, 6075
Bsp120I GGGCCC 1 cut(s) 5961
Bsp1407I TGTACA 2 cut(s) 482, 1405
Bsp1720I GCTNAGC 2 cut(s) 1587, 4035
Bsp19I CCATGG 3 cut(s) 276, 373, 2487
BspACI CCGC 7 cut(s) 90, 144, 2866, 3086, 3332, 4168, 6490
BspHI TCATGA 5 cut(s) 1648, 3105, 3727, 3895, 5812
BspLI GGNNCC 5 cut(s) 820, 2504, 3199, 5963, 6516
BspMAI CTGCAG 4 cut(s) 1858, 2835, 2924, 3303
BspMI ACCTGC 1 cut(s) 130
BspPI GGATC 6 cut(s) 17, 188, 3135, 3288, 5525, 6509
BspQI GCTCTTC 8 cut(s) 289, 1101, 2979, 3840, 4113, 4683, 5496, 5743
BspT107I GGYRCC 1 cut(s) 2502
BspTI CTTAAG 5 cut(s) 719, 944, 5249, 5332, 5971
BspTNI GGTCTC 4 cut(s) 780, 3695, 3827, 6075
BsrBI CCGCTC 1 cut(s) 6492
BsrFI RCCGGY 1 cut(s) 431
BsrGI TGTACA 2 cut(s) 482, 1405
BsrI ACTGG 8 cut(s) 294, 822, 1340, 1351, 2968, 3763, 6235, 6516
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 9 cut(s) 276, 301, 373, 2382, 2487, 2952, 3168, 4026, 5052
BssNAI GTATAC 1 cut(s) 1725
BssT1I CCWWGG 6 cut(s) 276, 301, 373, 2382, 2487, 5052
Bst1107I GTATAC 1 cut(s) 1725
BstAFI CTTAAG 5 cut(s) 719, 944, 5249, 5332, 5971
BstAPI GCANNNNNTGC 2 cut(s) 1426, 4295
BstAUI TGTACA 2 cut(s) 482, 1405
BstC8I GCNNGC 6 cut(s) 1378, 1879, 1983, 3943, 5190, 6213
BstDSI CCRYGG 3 cut(s) 276, 373, 2487
BstEII GGTNACC 1 cut(s) 271
BstENI CCTNNNNNAGG 2 cut(s) 1826, 3332
BstH2I RGCGCY 1 cut(s) 5309
BstHHI GCGC 2 cut(s) 4982, 5308
BstNSI RCATGY 3 cut(s) 183, 404, 6325
BstPI GGTNACC 1 cut(s) 271
BstSFI CTRYAG 7 cut(s) 633, 1854, 2372, 2688, 2831, 2920, 3299
BstSLI GKGCMC 4 cut(s) 1879, 4597, 5111, 5965
BstV2I GAAGAC 5 cut(s) 38, 528, 2643, 3495, 3969
BstX2I RGATCY 7 cut(s) 193, 1099, 2156, 3127, 3565, 5517, 6514
BstXI CCANNNNNNTGG 3 cut(s) 1900, 2102, 3979
BstYI RGATCY 7 cut(s) 193, 1099, 2156, 3127, 3565, 5517, 6514
BstZ17I GTATAC 1 cut(s) 1725
BsuI GTATCC 5 cut(s) 523, 630, 4302, 4872, 5869
BtgI CCRYGG 3 cut(s) 276, 373, 2487
BtgZI GCGATG 1 cut(s) 1233
BtrI CACGTC 1 cut(s) 5911
BtsI GCAGTG 8 cut(s) 362, 1905, 2443, 2929, 3570, 4953, 6160, 6285
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 6 cut(s) 1378, 1879, 1983, 3943, 5190, 6213
CaiI CAGNNNCTG 2 cut(s) 1346, 3604
CciI TCATGA 5 cut(s) 1648, 3105, 3727, 3895, 5812
CfoI GCGC 2 cut(s) 4982, 5308
Cfr10I RCCGGY 1 cut(s) 431
CseI GACGC 1 cut(s) 330
Csp6I GTAC 9 cut(s) 410, 483, 726, 883, 1268, 1406, 2515, 3555, 6176
CspAI ACCGGT 1 cut(s) 431
CviQI GTAC 9 cut(s) 410, 483, 726, 883, 1268, 1406, 2515, 3555, 6176
DraI TTTAAA 1 cut(s) 1335
DriI GACNNNNNGTC 1 cut(s) 1285
EaeI YGGCCR 3 cut(s) 4126, 4411, 4696
Eam1105I GACNNNNNGTC 1 cut(s) 1285
Ecl136II GAGCTC 4 cut(s) 463, 1834, 5736, 6310
Eco130I CCWWGG 6 cut(s) 276, 301, 373, 2382, 2487, 5052
Eco24I GRGCYC 7 cut(s) 284, 465, 835, 1836, 5738, 5965, 6312
Eco31I GGTCTC 4 cut(s) 780, 3695, 3827, 6075
Eco47I GGWCC 8 cut(s) 291, 819, 1287, 1903, 1918, 1942, 3325, 3634
Eco53kI GAGCTC 4 cut(s) 463, 1834, 5736, 6310
Eco91I GGTNACC 1 cut(s) 271
EcoICRI GAGCTC 4 cut(s) 463, 1834, 5736, 6310
EcoNI CCTNNNNNAGG 2 cut(s) 1826, 3332
EcoO109I RGGNCCY 6 cut(s) 1287, 1819, 1918, 1942, 3325, 5961
EcoO65I GGTNACC 1 cut(s) 271
EcoRI GAATTC 2 cut(s) 1131, 5216
EcoT14I CCWWGG 6 cut(s) 276, 301, 373, 2382, 2487, 5052
EcoT22I ATGCAT 5 cut(s) 1956, 4053, 5160, 6166, 6378
EcoT38I GRGCYC 7 cut(s) 284, 465, 835, 1836, 5738, 5965, 6312
ErhI CCWWGG 6 cut(s) 276, 301, 373, 2382, 2487, 5052
FaqI GGGAC 4 cut(s) 832, 1292, 1378, 2071
FauNDI CATATG 2 cut(s) 2815, 5581
FbaI TGATCA 7 cut(s) 796, 3102, 3613, 3853, 4957, 5082, 6043
FblI GTMKAC 2 cut(s) 898, 1724
FriOI GRGCYC 7 cut(s) 284, 465, 835, 1836, 5738, 5965, 6312
GlaI GCGC 2 cut(s) 4981, 5307
GsaI CCCAGC 1 cut(s) 844
GsuI CTGGAG 8 cut(s) 679, 879, 2351, 3491, 4778, 5021, 5147, 6347
HaeII RGCGCY 1 cut(s) 5309
HapII CCGG 1 cut(s) 432
HgaI GACGC 1 cut(s) 330
HhaI GCGC 2 cut(s) 4982, 5308
Hin6I GCGC 2 cut(s) 4980, 5306
HinP1I GCGC 2 cut(s) 4980, 5306
HincII GTYRAC 2 cut(s) 1785, 3270
HindII GTYRAC 2 cut(s) 1785, 3270
HindIII AAGCTT 9 cut(s) 23, 2008, 3179, 3461, 4196, 4481, 4766, 5186, 5968
HpaI GTTAAC 1 cut(s) 1785
HpaII CCGG 1 cut(s) 432
Hpy99I CGWCG 1 cut(s) 1265
HpyCH4IV ACGT 4 cut(s) 488, 802, 3522, 5910
HpySE526I ACGT 4 cut(s) 488, 802, 3522, 5910
HspAI GCGC 2 cut(s) 4980, 5306
Ksp22I TGATCA 7 cut(s) 796, 3102, 3613, 3853, 4957, 5082, 6043
KspAI GTTAAC 1 cut(s) 1785
LguI GCTCTTC 8 cut(s) 289, 1101, 2979, 3840, 4113, 4683, 5496, 5743
MaeII ACGT 4 cut(s) 488, 802, 3522, 5910
MbiI CCGCTC 1 cut(s) 6492
MflI RGATCY 7 cut(s) 193, 1099, 2156, 3127, 3565, 5517, 6514
MlsI TGGCCA 3 cut(s) 4128, 4413, 4698
MluNI TGGCCA 3 cut(s) 4128, 4413, 4698
MmeI TCCRAC 6 cut(s) 2697, 3267, 3784, 4057, 5076, 5985
Mox20I TGGCCA 3 cut(s) 4128, 4413, 4698
Mph1103I ATGCAT 5 cut(s) 1956, 4053, 5160, 6166, 6378
MroXI GAANNNNTTC 5 cut(s) 2475, 2580, 2640, 2988, 5688
MscI TGGCCA 3 cut(s) 4128, 4413, 4698
MslI CAYNNNNRTG 6 cut(s) 184, 378, 1413, 1898, 3909, 6267
Msp20I TGGCCA 3 cut(s) 4128, 4413, 4698
MspA1I CMGCKG 5 cut(s) 923, 2329, 3086, 5315, 5807
MspCI CTTAAG 5 cut(s) 719, 944, 5249, 5332, 5971
MspI CCGG 1 cut(s) 432
Mva1269I GAATGC 3 cut(s) 1708, 5342, 5822
NcoI CCATGG 3 cut(s) 276, 373, 2487
NdeI CATATG 2 cut(s) 2815, 5581
NlaIV GGNNCC 5 cut(s) 820, 2504, 3199, 5963, 6516
NmuCI GTSAC 8 cut(s) 271, 1500, 2260, 2438, 4151, 4436, 4721, 5921
NsiI ATGCAT 5 cut(s) 1956, 4053, 5160, 6166, 6378
NspI RCATGY 3 cut(s) 183, 404, 6325
PagI TCATGA 5 cut(s) 1648, 3105, 3727, 3895, 5812
PciI ACATGT 1 cut(s) 400
PciSI GCTCTTC 8 cut(s) 289, 1101, 2979, 3840, 4113, 4683, 5496, 5743
PctI GAATGC 3 cut(s) 1708, 5342, 5822
PdmI GAANNNNTTC 5 cut(s) 2475, 2580, 2640, 2988, 5688
Pfl23II CGTACG 1 cut(s) 882
PflMI CCANNNNNTGG 5 cut(s) 828, 1346, 2464, 2569, 3025
PinAI ACCGGT 1 cut(s) 431
PpuMI RGGWCCY 4 cut(s) 1287, 1918, 1942, 3325
PscI ACATGT 1 cut(s) 400
PshBI ATTAAT 5 cut(s) 4250, 4535, 4820, 5448, 5615
PsiI TTATAA 1 cut(s) 5748
Psp124BI GAGCTC 4 cut(s) 465, 1836, 5738, 6312
Psp1406I AACGTT 1 cut(s) 802
Psp5II RGGWCCY 4 cut(s) 1287, 1918, 1942, 3325
PspEI GGTNACC 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 840
PspLI CGTACG 1 cut(s) 882
PspN4I GGNNCC 5 cut(s) 820, 2504, 3199, 5963, 6516
PspOMI GGGCCC 1 cut(s) 5961
PspPPI RGGWCCY 4 cut(s) 1287, 1918, 1942, 3325
PsrI GAACNNNNNNTAC 2 cut(s) 3114, 3146
PstI CTGCAG 4 cut(s) 1858, 2835, 2924, 3303
PstNI CAGNNNCTG 2 cut(s) 1346, 3604
PsuI RGATCY 7 cut(s) 193, 1099, 2156, 3127, 3565, 5517, 6514
PvuII CAGCTG 4 cut(s) 923, 2329, 5315, 5807
RsaI GTAC 9 cut(s) 411, 484, 727, 884, 1269, 1407, 2516, 3556, 6177
RsaNI GTAC 9 cut(s) 410, 483, 726, 883, 1268, 1406, 2515, 3555, 6176
RseI CAYNNNNRTG 6 cut(s) 184, 378, 1413, 1898, 3909, 6267
SacI GAGCTC 4 cut(s) 465, 1836, 5738, 6312
SapI GCTCTTC 8 cut(s) 289, 1101, 2979, 3840, 4113, 4683, 5496, 5743
ScaI AGTACT 1 cut(s) 6177
SfcI CTRYAG 7 cut(s) 633, 1854, 2372, 2688, 2831, 2920, 3299
SinI GGWCC 8 cut(s) 291, 819, 1287, 1903, 1918, 1942, 3325, 3634
SmiMI CAYNNNNRTG 6 cut(s) 184, 378, 1413, 1898, 3909, 6267
SpeI ACTAGT 1 cut(s) 4256
SsiI CCGC 7 cut(s) 90, 144, 2866, 3086, 3332, 4168, 6490
SspI AATATT 2 cut(s) 2746, 2839
SstI GAGCTC 4 cut(s) 465, 1836, 5738, 6312
StyI CCWWGG 6 cut(s) 276, 301, 373, 2382, 2487, 5052
TaiI ACGT 4 cut(s) 491, 805, 3525, 5913
TaqI TCGA 8 cut(s) 5, 772, 993, 1836, 2348, 5147, 5625, 6460
TaqII GACCGA 1 cut(s) 4856
TatI WGTACW 7 cut(s) 409, 482, 725, 1405, 2514, 3554, 6175
TauI GCSGC 1 cut(s) 6492
TseFI GTSAC 8 cut(s) 271, 1500, 2260, 2438, 4151, 4436, 4721, 5921
Tsp45I GTSAC 8 cut(s) 271, 1500, 2260, 2438, 4151, 4436, 4721, 5921
TspGWI ACGGA 1 cut(s) 5921
Van91I CCANNNNNTGG 5 cut(s) 828, 1346, 2464, 2569, 3025
Vha464I CTTAAG 5 cut(s) 719, 944, 5249, 5332, 5971
VneI GTGCAC 1 cut(s) 5107
VpaK11BI GGWCC 8 cut(s) 291, 819, 1287, 1903, 1918, 1942, 3325, 3634
VspI ATTAAT 5 cut(s) 4250, 4535, 4820, 5448, 5615
XagI CCTNNNNNAGG 2 cut(s) 1826, 3332
XceI RCATGY 3 cut(s) 183, 404, 6325
XmiI GTMKAC 2 cut(s) 898, 1724
XmnI GAANNNNTTC 5 cut(s) 2475, 2580, 2640, 2988, 5688
ZrmI AGTACT 1 cut(s) 6177
Zsp2I ATGCAT 5 cut(s) 1956, 4053, 5160, 6166, 6378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.