AT1G23460

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004713
Physical Location & Seq
Forward (+)
8326747 .. 8329782
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G23460.1

Sequence Viewer

Length: 1383 bp
ATGATGGATAAGCTCTTCATTCTTTCTCTTCTAGGTCTGTTATTGGTAACTGCTTATGGAGCTGCAGGGAAGATGGTGTACACAGATCTTGACATTCTTGACGAGCTTGAGAACTTTGATGTACTGGTAGATGATGATGATGATACAAAGCTCTTGGATTGGCCATCATTCACAAGCCGGCATTCTGGTAAAAATCTGGTGAATGTTGATACATTCGGTGCAGCTGGAGATGGAGTTTCTGATGATACTCAGGCATTCGTAAGCGCATGGAGCAAAGCCTGCAGTACATCAAAATCTGTGTTTCTGGTTCCAGAAGGACGTCGATATTTAGTAAACGCAACAAAGTTCAATGGTCCATGTGAACAAAAGTTGATCATTCAGATTGACGGAACAATAGTGGCACCAGATGAGCCCAGCAATTGGGACTCAAAGTTCCAGAGAATTTGGCTCGAGTTTTCAAAGCTCAAAGGGGTCGTGTTTCAAGGGAAAGGAGTTATAGATGGTTCAGGCAGCAAATGGTGGGCTGCTTCTTGCAAGAAAAACAAGTCTAATCCTTGCAAAAGCGCACCAACTGCACTAACTATAGAATCTAGTTCAGGTGTGAAAGTAAGTGGCTTGACAATCCAGAACAGCCAGCAGATGAATTTCATCATAGCAAGGTCAGATTCAGTTCGAGTCTCCAAGGTTATGGTCTCTTCTCCAGGAGATAGTCCCAACACTGATGGAATCCATATCACTGGATCTACCAATGTTATCCTTCAAGACTGTAAAATCGGCACAGGGGATGATTGTGTCTCGATTGTGAATGCCAGCTCGAATATAAAGATGAAGAACATCTATTGTGGACCTGGACATGGAATCAGTATTGGAAGTCTTGGGAAGGATAATACGACAGGCATTGTAACACAAGTTGTGTTGGACACTGCCTTATTGAGAGAAACAACTAATGGACTCAGAATAAAAACATATCAGGGAGGTTCTGGTTACGTTCAGGGTATTCGATTCACAAACGTAGAGATGCAAGACGTCGCAAACCCGATACTCATTGATCAGTTCTACTGTGATTCTCCAACTACATGTCAAAACCAGACCTCGGCGGTTAAAATCAGCCAGATAATGTACCGGAACATAACCGGGACAACCAAAAGTGCGAAAGCCATCAAATTTGCGTGCAGCGACACAGTCCCTTGCAGCCACATAGTCCTCAACAATGTGAATCTTGAAGGCAACGACGGTCAAGTCGAAGCTTACTGTAATTCAGCCGAAGGTTTTGGGTATGGAGTGATTCATCCATCAGCGGACTGTCTCTACTCGCACGACGACAAGGGCTTGGACCAGACTCACAAGTCGGAGGAAGCCGAGACTGGCCACGACGAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

460

Amino Acids

49.57

Weight (kDa)

5.1

Isoelectric Point (pI)

27.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 96 - 420 3.2e-93 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1238, 1345
AatII GACGTC 2 cut(s) 322, 1029
AccB1I GGYRCC 1 cut(s) 400
AccB7I CCANNNNNTGG 1 cut(s) 420
AciI CCGC 2 cut(s) 1097, 1298
AclWI GGATC 1 cut(s) 748
AcoI YGGCCR 2 cut(s) 161, 1366
AcsI RAATTY 3 cut(s) 441, 643, 1163
AcyI GRCGYC 2 cut(s) 319, 1026
AfaI GTAC 4 cut(s) 80, 123, 286, 1121
AfiI CCNNNNNNNGG 3 cut(s) 420, 854, 1093
AflIII ACRYGT 1 cut(s) 1076
AgsI TTSAA 5 cut(s) 349, 459, 482, 761, 1223
AjnI CCWGG 2 cut(s) 700, 847
AloI GAACNNNNNNTCC 2 cut(s) 416, 448
AluBI AGCT 9 cut(s) 13, 62, 106, 151, 224, 463, 813, 1247, 1378
AluI AGCT 9 cut(s) 13, 62, 106, 151, 224, 463, 813, 1247, 1378
Alw26I GTCTC 5 cut(s) 682, 697, 799, 1310, 1355
AlwI GGATC 1 cut(s) 748
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 2 cut(s) 161, 1366
ApeKI GCWGC 6 cut(s) 62, 221, 510, 524, 1173, 1191
ApoI RAATTY 3 cut(s) 441, 643, 1163
AspLEI GCGC 2 cut(s) 266, 566
AspS9I GGNCC 3 cut(s) 353, 845, 1333
AsuC2I CCSGG 1 cut(s) 1135
AsuHPI GGTGA 1 cut(s) 211
AvaI CYCGRG 1 cut(s) 449
AvaII GGWCC 3 cut(s) 353, 845, 1333
BalI TGGCCA 2 cut(s) 163, 1368
BanI GGYRCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 414
BarI GAAGNNNNNNTAC 2 cut(s) 62, 94
BbvI GCAGC 6 cut(s) 49, 233, 511, 522, 1185, 1203
BccI CCATC 7 cut(s) 67, 172, 224, 494, 716, 1166, 1300
BciT130I CCWGG 2 cut(s) 702, 849
BclI TGATCA 2 cut(s) 372, 1048
BcnI CCSGG 1 cut(s) 1135
BcoDI GTCTC 5 cut(s) 682, 697, 799, 1310, 1355
BfaI CTAG 2 cut(s) 32, 591
BfmI CTRYAG 3 cut(s) 63, 280, 582
BglII AGATCT 1 cut(s) 85
BisI GCNGC 6 cut(s) 63, 222, 511, 525, 1174, 1192
BlsI GCNGC 6 cut(s) 64, 223, 512, 526, 1175, 1193
Bme1390I CCNGG 3 cut(s) 702, 849, 1135
Bme18I GGWCC 3 cut(s) 353, 845, 1333
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 3 cut(s) 353, 845, 1333
BmiI GGNNCC 2 cut(s) 309, 402
BmrFI CCNGG 3 cut(s) 702, 849, 1135
BmsI GCATC 1 cut(s) 1008
BpmI CTGGAG 2 cut(s) 246, 684
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 1135
BsaHI GRCGYC 2 cut(s) 319, 1026
BsaI GGTCTC 1 cut(s) 697
BsaJI CCNNGG 2 cut(s) 681, 1092
BsaWI WCCGGW 1 cut(s) 1122
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 3 cut(s) 420, 854, 1093
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 3 cut(s) 129, 742, 1369
BseBI CCWGG 2 cut(s) 702, 849
BseDI CCNNGG 2 cut(s) 681, 1092
BseGI GGATG 2 cut(s) 790, 1288
BseLI CCNNNNNNNGG 3 cut(s) 420, 854, 1093
BseMII CTCAG 2 cut(s) 263, 967
BseNI ACTGG 3 cut(s) 129, 742, 1369
BseXI GCAGC 6 cut(s) 49, 233, 511, 522, 1185, 1203
BseYI CCCAGC 1 cut(s) 413
BsgI GTGCAG 3 cut(s) 240, 558, 1192
BshFI GGCC 2 cut(s) 163, 1368
BshNI GGYRCC 1 cut(s) 400
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 3 cut(s) 178, 1123, 1134
BslFI GGGAC 4 cut(s) 437, 696, 1150, 1169
BslI CCNNNNNNNGG 3 cut(s) 420, 854, 1093
BsmAI GTCTC 5 cut(s) 682, 697, 799, 1310, 1355
BsmFI GGGAC 4 cut(s) 437, 696, 1150, 1169
BsmI GAATGC 3 cut(s) 181, 254, 811
BsnI GGCC 2 cut(s) 163, 1368
Bso31I GGTCTC 1 cut(s) 697
BsoBI CYCGRG 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 414
Bsp1407I TGTACA 1 cut(s) 78
Bsp143I GATC 4 cut(s) 85, 372, 740, 1048
BspACI CCGC 2 cut(s) 1097, 1298
BspANI GGCC 2 cut(s) 163, 1368
BspCNI CTCAG 2 cut(s) 262, 966
BspLI GGNNCC 2 cut(s) 309, 402
BspMAI CTGCAG 2 cut(s) 67, 284
BspPI GGATC 1 cut(s) 748
BspQI GCTCTTC 1 cut(s) 20
BspT107I GGYRCC 1 cut(s) 400
BspTNI GGTCTC 1 cut(s) 697
BsrFI RCCGGY 1 cut(s) 177
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 3 cut(s) 129, 742, 1369
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 681, 1092
BssMI GATC 4 cut(s) 85, 372, 740, 1048
BssNI GRCGYC 2 cut(s) 319, 1026
BssT1I CCWWGG 1 cut(s) 681
Bst2UI CCWGG 2 cut(s) 702, 849
Bst4CI ACNGT 6 cut(s) 767, 1061, 1183, 1235, 1253, 1304
Bst6I CTCTTC 3 cut(s) 20, 33, 700
BstACI GRCGYC 2 cut(s) 319, 1026
BstAPI GCANNNNNTGC 2 cut(s) 279, 572
BstAUI TGTACA 1 cut(s) 78
BstC8I GCNNGC 5 cut(s) 179, 280, 635, 811, 1171
BstDEI CTNAG 2 cut(s) 249, 953
BstF5I GGATG 2 cut(s) 790, 1288
BstHHI GCGC 2 cut(s) 266, 566
BstKTI GATC 4 cut(s) 88, 375, 743, 1051
BstMAI GTCTC 5 cut(s) 682, 697, 799, 1310, 1355
BstMBI GATC 4 cut(s) 85, 372, 740, 1048
BstMWI GCNNNNNNNGC 4 cut(s) 59, 270, 279, 572
BstNI CCWGG 2 cut(s) 702, 849
BstNSI RCATGY 1 cut(s) 1080
BstSCI CCNGG 3 cut(s) 700, 847, 1133
BstSFI CTRYAG 3 cut(s) 63, 280, 582
BstV1I GCAGC 6 cut(s) 49, 233, 511, 522, 1185, 1203
BstX2I RGATCY 2 cut(s) 85, 740
BstXI CCANNNNNNTGG 2 cut(s) 688, 737
BstYI RGATCY 2 cut(s) 85, 740
BsuRI GGCC 2 cut(s) 163, 1368
BtsCI GGATG 2 cut(s) 790, 1288
BtsI GCAGTG 1 cut(s) 921
BtsIMutI CAGTG 3 cut(s) 717, 735, 921
Cac8I GCNNGC 5 cut(s) 179, 280, 635, 811, 1171
CfoI GCGC 2 cut(s) 266, 566
Cfr10I RCCGGY 1 cut(s) 177
Cfr13I GGNCC 3 cut(s) 353, 845, 1333
Csp6I GTAC 4 cut(s) 79, 122, 285, 1120
CviAII CATG 4 cut(s) 267, 357, 854, 1077
CviQI GTAC 4 cut(s) 79, 122, 285, 1120
DdeI CTNAG 2 cut(s) 249, 953
DpnI GATC 4 cut(s) 87, 374, 742, 1050
DpnII GATC 4 cut(s) 85, 372, 740, 1048
DrdI GACNNNNNNGTC 2 cut(s) 1238, 1345
DseDI GACNNNNNNGTC 2 cut(s) 1238, 1345
EaeI YGGCCR 2 cut(s) 161, 1366
Eam1104I CTCTTC 3 cut(s) 20, 33, 700
EarI CTCTTC 3 cut(s) 20, 33, 700
Eco130I CCWWGG 1 cut(s) 681
Eco24I GRGCYC 1 cut(s) 414
Eco31I GGTCTC 1 cut(s) 697
Eco47I GGWCC 3 cut(s) 353, 845, 1333
Eco88I CYCGRG 1 cut(s) 449
EcoRII CCWGG 2 cut(s) 700, 847
EcoT14I CCWWGG 1 cut(s) 681
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 681
FaeI CATG 4 cut(s) 270, 360, 857, 1080
FaqI GGGAC 4 cut(s) 437, 696, 1150, 1169
FatI CATG 4 cut(s) 266, 356, 853, 1076
FbaI TGATCA 2 cut(s) 372, 1048
Fnu4HI GCNGC 6 cut(s) 63, 222, 511, 525, 1174, 1192
FokI GGATG 2 cut(s) 797, 1275
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 6 cut(s) 63, 222, 511, 525, 1174, 1192
FspBI CTAG 2 cut(s) 32, 591
GlaI GCGC 2 cut(s) 265, 565
GluI GCNGC 6 cut(s) 63, 222, 511, 525, 1174, 1192
GsaI CCCAGC 1 cut(s) 417
GsuI CTGGAG 2 cut(s) 246, 684
HaeIII GGCC 2 cut(s) 163, 1368
HapII CCGG 3 cut(s) 178, 1123, 1134
HhaI GCGC 2 cut(s) 266, 566
Hin1I GRCGYC 2 cut(s) 319, 1026
Hin1II CATG 4 cut(s) 270, 360, 857, 1080
Hin6I GCGC 2 cut(s) 264, 564
HinP1I GCGC 2 cut(s) 264, 564
HindIII AAGCTT 1 cut(s) 1245
HpaII CCGG 3 cut(s) 178, 1123, 1134
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 5 cut(s) 79, 81, 334, 362, 845
Hpy188I TCNGA 5 cut(s) 241, 381, 664, 956, 1351
Hpy188III TCNNGA 8 cut(s) 89, 98, 311, 436, 625, 761, 796, 1220
Hpy8I GTNNAC 5 cut(s) 79, 81, 334, 362, 845
Hpy99I CGWCG 5 cut(s) 324, 1031, 1235, 1322, 1376
HpyAV CCTTC 5 cut(s) 308, 767, 874, 1217, 1259
HpyCH4III ACNGT 6 cut(s) 767, 1061, 1183, 1235, 1253, 1304
HpyCH4IV ACGT 4 cut(s) 319, 987, 1011, 1026
HpyCH4V TGCA 9 cut(s) 65, 221, 282, 534, 558, 575, 1021, 1173, 1191
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 270, 279, 572
HpyF3I CTNAG 2 cut(s) 249, 953
HpySE526I ACGT 4 cut(s) 319, 987, 1011, 1026
Hsp92I GRCGYC 2 cut(s) 319, 1026
Hsp92II CATG 4 cut(s) 270, 360, 857, 1080
HspAI GCGC 2 cut(s) 264, 564
KroI GCCGGC 1 cut(s) 177
KroNI GCCGGC 1 cut(s) 179
Ksp22I TGATCA 2 cut(s) 372, 1048
Kzo9I GATC 4 cut(s) 85, 372, 740, 1048
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 2 cut(s) 59, 270
Lsp1109I GCAGC 6 cut(s) 49, 233, 511, 522, 1185, 1203
LweI GCATC 1 cut(s) 1008
MaeI CTAG 2 cut(s) 32, 591
MaeII ACGT 4 cut(s) 319, 987, 1011, 1026
MaeIII GTNAC 3 cut(s) 46, 901, 983
MalI GATC 4 cut(s) 87, 374, 742, 1050
MboI GATC 4 cut(s) 85, 372, 740, 1048
MboII GAAGA 5 cut(s) 7, 20, 82, 687, 841
MfeI CAATTG 1 cut(s) 418
MflI RGATCY 2 cut(s) 85, 740
MhlI GDGCHC 1 cut(s) 414
MlsI TGGCCA 2 cut(s) 163, 1368
MluCI AATT 5 cut(s) 418, 441, 643, 1163, 1255
MluNI TGGCCA 2 cut(s) 163, 1368
MlyI GAGTC 4 cut(s) 419, 684, 945, 1333
MmeI TCCRAC 3 cut(s) 897, 1094, 1329
MnlI CCTC 4 cut(s) 968, 1102, 1214, 1345
Mox20I TGGCCA 2 cut(s) 163, 1368
MroNI GCCGGC 1 cut(s) 177
MscI TGGCCA 2 cut(s) 163, 1368
MseI TTAA 1 cut(s) 1101
Msp20I TGGCCA 2 cut(s) 163, 1368
MspA1I CMGCKG 2 cut(s) 224, 1298
MspI CCGG 3 cut(s) 178, 1123, 1134
MspR9I CCNGG 3 cut(s) 702, 849, 1135
MunI CAATTG 1 cut(s) 418
Mva1269I GAATGC 3 cut(s) 181, 254, 811
MvaI CCWGG 2 cut(s) 702, 849
MwoI GCNNNNNNNGC 4 cut(s) 59, 270, 279, 572
NaeI GCCGGC 1 cut(s) 179
NciI CCSGG 1 cut(s) 1135
NdeII GATC 4 cut(s) 85, 372, 740, 1048
NgoMIV GCCGGC 1 cut(s) 177
NlaIII CATG 4 cut(s) 270, 360, 857, 1080
NlaIV GGNNCC 2 cut(s) 309, 402
NmeAIII GCCGAG 1 cut(s) 1073
NspI RCATGY 1 cut(s) 1080
PaeR7I CTCGAG 1 cut(s) 449
PciI ACATGT 1 cut(s) 1076
PciSI GCTCTTC 1 cut(s) 20
PcsI WCGNNNNNNNCGW 1 cut(s) 1239
PctI GAATGC 3 cut(s) 181, 254, 811
PdiI GCCGGC 1 cut(s) 179
PfeI GAWTC 8 cut(s) 587, 665, 726, 858, 1002, 1064, 1216, 1285
PflFI GACNNNGTC 1 cut(s) 1181
PflMI CCANNNNNTGG 1 cut(s) 420
PfoI TCCNGGA 1 cut(s) 700
PkrI GCNGC 6 cut(s) 64, 223, 512, 526, 1175, 1193
PleI GAGTC 4 cut(s) 419, 683, 945, 1333
PpsI GAGTC 4 cut(s) 419, 683, 945, 1333
PscI ACATGT 1 cut(s) 1076
Psp6I CCWGG 2 cut(s) 700, 847
PspFI CCCAGC 1 cut(s) 413
PspGI CCWGG 2 cut(s) 700, 847
PspN4I GGNNCC 2 cut(s) 309, 402
PspPI GGNCC 3 cut(s) 353, 845, 1333
PspXI VCTCGAGB 1 cut(s) 449
PstI CTGCAG 2 cut(s) 67, 284
PsuI RGATCY 2 cut(s) 85, 740
PsyI GACNNNGTC 1 cut(s) 1181
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 4 cut(s) 80, 123, 286, 1121
RsaNI GTAC 4 cut(s) 79, 122, 285, 1120
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 1 cut(s) 1101
SatI GCNGC 6 cut(s) 63, 222, 511, 525, 1174, 1192
Sau3AI GATC 4 cut(s) 85, 372, 740, 1048
Sau96I GGNCC 3 cut(s) 353, 845, 1333
SchI GAGTC 4 cut(s) 419, 684, 945, 1333
ScrFI CCNGG 3 cut(s) 702, 849, 1135
SduI GDGCHC 1 cut(s) 414
SfaNI GCATC 1 cut(s) 1008
SfcI CTRYAG 3 cut(s) 63, 280, 582
Sfr274I CTCGAG 1 cut(s) 449
SinI GGWCC 3 cut(s) 353, 845, 1333
SlaI CTCGAG 1 cut(s) 449
SmlI CTYRAG 2 cut(s) 107, 449
SmoI CTYRAG 2 cut(s) 107, 449
Sse9I AATT 5 cut(s) 418, 441, 643, 1163, 1255
SsiI CCGC 2 cut(s) 1097, 1298
SspMI CTAG 2 cut(s) 32, 591
StyD4I CCNGG 3 cut(s) 700, 847, 1133
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 6 cut(s) 767, 1061, 1183, 1235, 1253, 1304
TaiI ACGT 4 cut(s) 322, 990, 1014, 1029
TaqI TCGA 7 cut(s) 322, 450, 673, 797, 815, 1000, 1242
TasI AATT 5 cut(s) 418, 441, 643, 1163, 1255
TatI WGTACW 3 cut(s) 78, 121, 284
TfiI GAWTC 8 cut(s) 587, 665, 726, 858, 1002, 1064, 1216, 1285
Tru1I TTAA 1 cut(s) 1101
Tru9I TTAA 1 cut(s) 1101
TscAI CASTG 3 cut(s) 724, 742, 928
TseI GCWGC 6 cut(s) 62, 221, 510, 524, 1173, 1191
TspDTI ATGAA 5 cut(s) 7, 637, 656, 842, 1277
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 3 cut(s) 724, 742, 928
Tth111I GACNNNGTC 1 cut(s) 1181
Van91I CCANNNNNTGG 1 cut(s) 420
VpaK11BI GGWCC 3 cut(s) 353, 845, 1333
XapI RAATTY 3 cut(s) 441, 643, 1163
XceI RCATGY 1 cut(s) 1080
XhoI CTCGAG 1 cut(s) 449
XspI CTAG 2 cut(s) 32, 591
ZraI GACGTC 2 cut(s) 320, 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.