Rh4DG395200
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004713
Physical Location & Seq
Reverse (-)
61199520 .. 61202230
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG395200.1

Sequence Viewer

Length: 1392 bp
ATGGGTAAATTCTTCGTGATTTCTTTTCTTGGTATGCTGATAGCAGTTTATGGAGTTGCAGGAAACATTGTTTATGATGACATTGGCATTCTTGATGAGCTTGAAAGCTTTGACATAGAAGAAGACAATGAGGTGGACATCTTTGAGATTCCGTCATGGACAAGTGAACGTGGTAGCAAGGTTCTTGTTAATGTGGATAGCTTTGGTGCTACTGGAGATGGAGTTTCTGATGATACTCAGGCTTTTGTAAAAGCTTGGGATATTGCATGCAATACAACAAAAGCTGTTTTCCTGGTACCCCAAGGACGCCGCTATCTAGTAAATGCAACAAGATTTGAAGGGCCATGTGCAGATGGCTTAGTCATCCAGATTGAGGGAACAATTGTAGCACCGGATGATCCTAACAACTGGGATCCAAAATTTCCACGAACATGGCTTGACTTCTATAAATTGAATGGAGTACTTTTCCAAGGGCATGGAGTTATTGATGGATCAGGCAGTAAATGGTGGGCTTCATCCTGCAAAAAGAATAAGACAAATCCTTGCAGGAGTGCTCCTACAGCATTTACTATTGATAGGAGCTCAGCTATAAAGATAAAAGGGCTCACCATCCAGAACAGCCAACAAATGCATTTTACTATCTCTCAGAGTGACTCCGTACGAGTTTATGCAGTCCAAGTCTCGGCTCCAGGAGACAGTCCTAACACTGATGGAATCCATATCACTGCATCTACTAATGTGGTTCTCCAAGACTGCAAAATTGGAACAGGAGATGACTGCATCTCAATTGTCAATGCTACATCAAATATTAAGATGAAGAGAATCTTTTGCGGACCAGGACATGGAGTCAGCATCGGAAGTCTAGGGAAGGACAACTCCAGCGGCATAGTGACAAAAATTGTCTTGGATACAGCATACCTCAAGGAGACTACCAATGGCCTCCGCATTAAGACTTGGCAGGGAGGTTCTGGTTATGTTCGTGGGGTCCGTTTCCAAAACGTAAGGATGGAAGATGTAGCAAATCCCATAATCATAGATCAGTTTTACTGTGACTCCCCCAAAGCTTGTCAAAATCAGACATCAGCTGTGGAAATAAGCCAAATCATGTATCGGAACATCTCGGGAACCACAACGAGTAAGAATGCCATGAAGTTTGCCTGCAGTGACACAGTTCCATGCAGCAACCTAGTCCTCAGTAATGTCAACCTGGAGAAGAAGGACGGCACTGTGGAGACATATTGCAACTCTGCCGAAGGCTTCGGGACTGGAGTTGTGCATCCATCAGCAGACTGTCTGAATTCTCATGACAAAGACTTGACAATTATCAATCAGCCTACAGAAGTTAAAGATGCAGATCTCAGCATAGAAGATATTGTGCATACTGAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

463

Amino Acids

50.25

Weight (kDa)

4.94

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 92 - 416 3.7e-92 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 295
AccB1I GGYRCC 1 cut(s) 295
AccB7I CCANNNNNTGG 1 cut(s) 842
AciI CCGC 4 cut(s) 310, 831, 882, 943
AclWI GGATC 4 cut(s) 392, 407, 420, 499
AcsI RAATTY 3 cut(s) 8, 419, 1297
AcyI GRCGYC 1 cut(s) 307
AfaI GTAC 3 cut(s) 297, 462, 660
AfiI CCNNNNNNNGG 3 cut(s) 373, 682, 842
AgsI TTSAA 3 cut(s) 104, 338, 454
AhdI GACNNNNNGTC 1 cut(s) 845
AjnI CCWGG 4 cut(s) 291, 688, 835, 1206
AluBI AGCT 9 cut(s) 100, 108, 201, 254, 284, 582, 587, 1064, 1085
AluI AGCT 9 cut(s) 100, 108, 201, 254, 284, 582, 587, 1064, 1085
Alw21I GWGCWC 2 cut(s) 556, 584
Alw26I GTCTC 4 cut(s) 685, 687, 920, 1226
AlwI GGATC 4 cut(s) 392, 407, 420, 499
Ama87I CYCGRG 1 cut(s) 1120
AoxI GGCC 2 cut(s) 341, 937
ApeKI GCWGC 1 cut(s) 1179
ApoI RAATTY 3 cut(s) 8, 419, 1297
Asp718I GGTACC 1 cut(s) 295
AspS9I GGNCC 3 cut(s) 341, 833, 985
AsuHPI GGTGA 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 1120
AvaII GGWCC 2 cut(s) 833, 985
BamHI GGATCC 1 cut(s) 412
BanI GGYRCC 1 cut(s) 295
BanII GRGCYC 2 cut(s) 584, 606
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 2 cut(s) 556, 584
BbvI GCAGC 1 cut(s) 1191
BccI CCATC 7 cut(s) 212, 347, 482, 617, 704, 1000, 1288
BceAI ACGGC 1 cut(s) 1237
BciT130I CCWGG 4 cut(s) 293, 690, 837, 1208
BciVI GTATCC 1 cut(s) 901
BcoDI GTCTC 4 cut(s) 685, 687, 920, 1226
BfaI CTAG 3 cut(s) 317, 863, 1187
BfmI CTRYAG 3 cut(s) 558, 1159, 1335
BfuI GTATCC 1 cut(s) 901
BglII AGATCT 1 cut(s) 1354
BisI GCNGC 3 cut(s) 310, 883, 1180
BlpI GCTNAGC 1 cut(s) 583
BlsI GCNGC 3 cut(s) 311, 884, 1181
BmcAI AGTACT 1 cut(s) 462
Bme1390I CCNGG 4 cut(s) 293, 690, 837, 1208
Bme18I GGWCC 2 cut(s) 833, 985
BmeRI GACNNNNNGTC 1 cut(s) 845
BmeT110I CYCGRG 1 cut(s) 1120
BmgT120I GGNCC 3 cut(s) 341, 833, 985
BmiI GGNNCC 5 cut(s) 297, 414, 687, 986, 1126
BmrFI CCNGG 4 cut(s) 293, 690, 837, 1208
BmrI ACTGGG 1 cut(s) 418
BmsI GCATC 5 cut(s) 737, 789, 861, 1285, 1339
BmuI ACTGGG 1 cut(s) 418
BpiI GAAGAC 1 cut(s) 129
BpmI CTGGAG 5 cut(s) 234, 672, 862, 1229, 1287
Bpu1102I GCTNAGC 1 cut(s) 583
BpuEI CTTGAG 1 cut(s) 905
BsaBI GATNNNNATC 1 cut(s) 1353
BsaHI GRCGYC 1 cut(s) 307
BsaJI CCNNGG 2 cut(s) 301, 469
BsaWI WCCGGW 1 cut(s) 391
BsaXI ACNNNNNCTCC 4 cut(s) 207, 237, 1037, 1067
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 682, 842
Bse1I ACTGG 3 cut(s) 217, 413, 1270
Bse8I GATNNNNATC 1 cut(s) 1353
BseBI CCWGG 4 cut(s) 293, 690, 837, 1208
BseDI CCNNGG 2 cut(s) 301, 469
BseGI GGATG 6 cut(s) 363, 400, 515, 609, 1011, 1276
BseJI GATNNNNATC 1 cut(s) 1353
BseLI CCNNNNNNNGG 3 cut(s) 373, 682, 842
BseMII CTCAG 5 cut(s) 251, 597, 659, 1207, 1372
BseNI ACTGG 3 cut(s) 217, 413, 1270
BseXI GCAGC 1 cut(s) 1191
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 2 cut(s) 343, 939
BshNI GGYRCC 1 cut(s) 295
BsiHKAI GWGCWC 2 cut(s) 556, 584
BsiHKCI CYCGRG 1 cut(s) 1120
BsiSI CCGG 1 cut(s) 392
BsiWI CGTACG 1 cut(s) 658
BslFI GGGAC 1 cut(s) 1276
BslI CCNNNNNNNGG 3 cut(s) 373, 682, 842
BsmAI GTCTC 4 cut(s) 685, 687, 920, 1226
BsmFI GGGAC 1 cut(s) 1276
BsmI GAATGC 2 cut(s) 87, 1147
BsnI GGCC 2 cut(s) 343, 939
BsoBI CYCGRG 1 cut(s) 1120
Bsp1286I GDGCHC 3 cut(s) 556, 584, 606
Bsp143I GATC 5 cut(s) 397, 412, 491, 1036, 1354
Bsp1720I GCTNAGC 1 cut(s) 583
BspACI CCGC 4 cut(s) 310, 831, 882, 943
BspANI GGCC 2 cut(s) 343, 939
BspCNI CTCAG 5 cut(s) 250, 596, 658, 1206, 1371
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 5 cut(s) 297, 414, 687, 986, 1126
BspMAI CTGCAG 1 cut(s) 1163
BspPI GGATC 4 cut(s) 392, 407, 420, 499
BspT107I GGYRCC 1 cut(s) 295
BsrI ACTGG 3 cut(s) 217, 413, 1270
BssECI CCNNGG 2 cut(s) 301, 469
BssMI GATC 5 cut(s) 397, 412, 491, 1036, 1354
BssNI GRCGYC 1 cut(s) 307
BssT1I CCWWGG 2 cut(s) 301, 469
Bst2UI CCWGG 4 cut(s) 293, 690, 837, 1208
Bst4CI ACNGT 5 cut(s) 698, 1049, 1171, 1228, 1292
Bst6I CTCTTC 1 cut(s) 812
BstACI GRCGYC 1 cut(s) 307
BstC8I GCNNGC 2 cut(s) 268, 1159
BstDEI CTNAG 6 cut(s) 237, 358, 583, 645, 1193, 1358
BstF5I GGATG 6 cut(s) 363, 400, 515, 609, 1011, 1276
BstKTI GATC 5 cut(s) 400, 415, 494, 1039, 1357
BstMAI GTCTC 4 cut(s) 685, 687, 920, 1226
BstMBI GATC 5 cut(s) 397, 412, 491, 1036, 1354
BstMWI GCNNNNNNNGC 1 cut(s) 560
BstNI CCWGG 4 cut(s) 293, 690, 837, 1208
BstNSI RCATGY 1 cut(s) 270
BstSCI CCNGG 4 cut(s) 291, 688, 835, 1206
BstSFI CTRYAG 3 cut(s) 558, 1159, 1335
BstV1I GCAGC 1 cut(s) 1191
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 2 cut(s) 412, 1354
BstXI CCANNNNNNTGG 2 cut(s) 432, 476
BstYI RGATCY 2 cut(s) 412, 1354
BsuI GTATCC 1 cut(s) 901
BsuRI GGCC 2 cut(s) 343, 939
BtsCI GGATG 6 cut(s) 363, 400, 515, 609, 1011, 1276
BtsI GCAGTG 2 cut(s) 723, 1168
BtsIMutI CAGTG 4 cut(s) 705, 723, 1168, 1224
Cac8I GCNNGC 2 cut(s) 268, 1159
CciI TCATGA 1 cut(s) 1303
Cfr13I GGNCC 3 cut(s) 341, 833, 985
CseI GACGC 1 cut(s) 315
Csp6I GTAC 3 cut(s) 296, 461, 659
CviQI GTAC 3 cut(s) 296, 461, 659
DdeI CTNAG 6 cut(s) 237, 358, 583, 645, 1193, 1358
DpnI GATC 5 cut(s) 399, 414, 493, 1038, 1356
DpnII GATC 5 cut(s) 397, 412, 491, 1036, 1354
DriI GACNNNNNGTC 1 cut(s) 845
Eam1104I CTCTTC 1 cut(s) 812
Eam1105I GACNNNNNGTC 1 cut(s) 845
EarI CTCTTC 1 cut(s) 812
Ecl136II GAGCTC 1 cut(s) 582
Eco130I CCWWGG 2 cut(s) 301, 469
Eco24I GRGCYC 2 cut(s) 584, 606
Eco47I GGWCC 2 cut(s) 833, 985
Eco53kI GAGCTC 1 cut(s) 582
Eco88I CYCGRG 1 cut(s) 1120
EcoICRI GAGCTC 1 cut(s) 582
EcoRI GAATTC 1 cut(s) 1297
EcoRII CCWGG 4 cut(s) 291, 688, 835, 1206
EcoT14I CCWWGG 2 cut(s) 301, 469
EcoT22I ATGCAT 1 cut(s) 633
EcoT38I GRGCYC 2 cut(s) 584, 606
ErhI CCWWGG 2 cut(s) 301, 469
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FaqI GGGAC 1 cut(s) 1276
Fnu4HI GCNGC 3 cut(s) 310, 883, 1180
FokI GGATG 6 cut(s) 350, 407, 502, 596, 1018, 1263
FriOI GRGCYC 2 cut(s) 584, 606
Fsp4HI GCNGC 3 cut(s) 310, 883, 1180
FspBI CTAG 3 cut(s) 317, 863, 1187
GluI GCNGC 3 cut(s) 310, 883, 1180
GsuI CTGGAG 5 cut(s) 234, 672, 862, 1229, 1287
HaeIII GGCC 2 cut(s) 343, 939
HapII CCGG 1 cut(s) 392
HgaI GACGC 1 cut(s) 315
Hin1I GRCGYC 1 cut(s) 307
HincII GTYRAC 1 cut(s) 1204
HindII GTYRAC 1 cut(s) 1204
HindIII AAGCTT 3 cut(s) 106, 252, 1062
HinfI GANTC 6 cut(s) 148, 653, 714, 822, 846, 1052
HpaII CCGG 1 cut(s) 392
HphI GGTGA 1 cut(s) 598
Hpy166II GTNNAC 3 cut(s) 136, 167, 1204
Hpy188I TCNGA 7 cut(s) 229, 648, 857, 1077, 1113, 1296, 1391
Hpy188III TCNNGA 7 cut(s) 16, 92, 367, 613, 1122, 1261, 1304
Hpy8I GTNNAC 3 cut(s) 136, 167, 1204
HpyAV CCTTC 4 cut(s) 332, 862, 1210, 1247
HpyCH4III ACNGT 5 cut(s) 698, 1049, 1171, 1228, 1292
HpyCH4IV ACGT 2 cut(s) 169, 999
HpyF10VI GCNNNNNNNGC 1 cut(s) 560
HpyF3I CTNAG 6 cut(s) 237, 358, 583, 645, 1193, 1358
HpySE526I ACGT 2 cut(s) 169, 999
Hsp92I GRCGYC 1 cut(s) 307
KpnI GGTACC 1 cut(s) 299
Kzo9I GATC 5 cut(s) 397, 412, 491, 1036, 1354
LmnI GCTCC 3 cut(s) 559, 579, 691
Lsp1109I GCAGC 1 cut(s) 1191
LweI GCATC 5 cut(s) 737, 789, 861, 1285, 1339
MaeI CTAG 3 cut(s) 317, 863, 1187
MaeII ACGT 2 cut(s) 169, 999
MaeIII GTNAC 4 cut(s) 650, 889, 1049, 1163
MalI GATC 5 cut(s) 399, 414, 493, 1038, 1356
MboI GATC 5 cut(s) 397, 412, 491, 1036, 1354
MboII GAAGA 7 cut(s) 4, 131, 134, 829, 1022, 1225, 1379
MfeI CAATTG 2 cut(s) 381, 786
MflI RGATCY 2 cut(s) 412, 1354
MhlI GDGCHC 3 cut(s) 556, 584, 606
MluCI AATT 9 cut(s) 8, 381, 419, 449, 759, 786, 897, 1297, 1320
MlyI GAGTC 3 cut(s) 647, 855, 1046
MnlI CCTC 6 cut(s) 124, 367, 929, 950, 956, 1202
Mph1103I ATGCAT 1 cut(s) 633
MseI TTAA 4 cut(s) 189, 810, 948, 1344
MslI CAYNNNNRTG 1 cut(s) 430
MspA1I CMGCKG 2 cut(s) 882, 1085
MspI CCGG 1 cut(s) 392
MspR9I CCNGG 4 cut(s) 293, 690, 837, 1208
MunI CAATTG 2 cut(s) 381, 786
Mva1269I GAATGC 2 cut(s) 87, 1147
MvaI CCWGG 4 cut(s) 293, 690, 837, 1208
MwoI GCNNNNNNNGC 1 cut(s) 560
NdeII GATC 5 cut(s) 397, 412, 491, 1036, 1354
NlaIV GGNNCC 5 cut(s) 297, 414, 687, 986, 1126
NmeAIII GCCGAG 1 cut(s) 662
NmuCI GTSAC 4 cut(s) 650, 889, 1049, 1163
NsiI ATGCAT 1 cut(s) 633
NspI RCATGY 1 cut(s) 270
PaeI GCATGC 1 cut(s) 270
PagI TCATGA 1 cut(s) 1303
PcsI WCGNNNNNNNCGW 1 cut(s) 985
PctI GAATGC 2 cut(s) 87, 1147
PfeI GAWTC 3 cut(s) 148, 714, 822
Pfl23II CGTACG 1 cut(s) 658
PflMI CCANNNNNTGG 1 cut(s) 842
PfoI TCCNGGA 1 cut(s) 688
PkrI GCNGC 3 cut(s) 311, 884, 1181
PleI GAGTC 3 cut(s) 647, 854, 1046
PpsI GAGTC 3 cut(s) 647, 854, 1046
Psp124BI GAGCTC 1 cut(s) 584
Psp6I CCWGG 4 cut(s) 291, 688, 835, 1206
PspGI CCWGG 4 cut(s) 291, 688, 835, 1206
PspLI CGTACG 1 cut(s) 658
PspN4I GGNNCC 5 cut(s) 297, 414, 687, 986, 1126
PspPI GGNCC 3 cut(s) 341, 833, 985
PstI CTGCAG 1 cut(s) 1163
PsuI RGATCY 2 cut(s) 412, 1354
PvuII CAGCTG 1 cut(s) 1085
RsaI GTAC 3 cut(s) 297, 462, 660
RsaNI GTAC 3 cut(s) 296, 461, 659
RseI CAYNNNNRTG 1 cut(s) 430
SacI GAGCTC 1 cut(s) 584
SaqAI TTAA 4 cut(s) 189, 810, 948, 1344
SatI GCNGC 3 cut(s) 310, 883, 1180
Sau3AI GATC 5 cut(s) 397, 412, 491, 1036, 1354
Sau96I GGNCC 3 cut(s) 341, 833, 985
ScaI AGTACT 1 cut(s) 462
SchI GAGTC 3 cut(s) 647, 855, 1046
ScrFI CCNGG 4 cut(s) 293, 690, 837, 1208
SduI GDGCHC 3 cut(s) 556, 584, 606
SfaNI GCATC 5 cut(s) 737, 789, 861, 1285, 1339
SfcI CTRYAG 3 cut(s) 558, 1159, 1335
SinI GGWCC 2 cut(s) 833, 985
SmiMI CAYNNNNRTG 1 cut(s) 430
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
SphI GCATGC 1 cut(s) 270
Sse9I AATT 9 cut(s) 8, 381, 419, 449, 759, 786, 897, 1297, 1320
SsiI CCGC 4 cut(s) 310, 831, 882, 943
SspI AATATT 1 cut(s) 808
SspMI CTAG 3 cut(s) 317, 863, 1187
SstI GAGCTC 1 cut(s) 584
StyD4I CCNGG 4 cut(s) 291, 688, 835, 1206
StyI CCWWGG 2 cut(s) 301, 469
TaaI ACNGT 5 cut(s) 698, 1049, 1171, 1228, 1292
TaiI ACGT 2 cut(s) 172, 1002
TasI AATT 9 cut(s) 8, 381, 419, 449, 759, 786, 897, 1297, 1320
TatI WGTACW 1 cut(s) 460
TauI GCSGC 2 cut(s) 312, 885
TfiI GAWTC 3 cut(s) 148, 714, 822
Tru1I TTAA 4 cut(s) 189, 810, 948, 1344
Tru9I TTAA 4 cut(s) 189, 810, 948, 1344
TscAI CASTG 4 cut(s) 712, 730, 1168, 1231
TseFI GTSAC 4 cut(s) 650, 889, 1049, 1163
TseI GCWGC 1 cut(s) 1179
Tsp45I GTSAC 4 cut(s) 650, 889, 1049, 1163
TspDTI ATGAA 3 cut(s) 504, 830, 1163
TspGWI ACGGA 3 cut(s) 141, 646, 977
TspRI CASTG 4 cut(s) 712, 730, 1168, 1231
Van91I CCANNNNNTGG 1 cut(s) 842
VpaK11BI GGWCC 2 cut(s) 833, 985
XapI RAATTY 3 cut(s) 8, 419, 1297
XceI RCATGY 1 cut(s) 270
XspI CTAG 3 cut(s) 317, 863, 1187
ZrmI AGTACT 1 cut(s) 462
Zsp2I ATGCAT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.