AT1G70500

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004713
Physical Location & Seq
Reverse (-)
26566398 .. 26568871
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70500.1

Sequence Viewer

Length: 1407 bp
ATGATTAAACCCTTTATTATTTCTCTGTTTGCTCTGTTGATGGTAGCTTCTTATGGAGCTACAGGAAAAATGTTGTTCAAGGAACTTGACATTCTTGGGAAGCTTGAGAACCTCGATGTACCTGAGGATGATATTGAGGATGATGTGACCTTCTTTGATTTCTCGTCGTTTACAAGCCAATATTCTGGTAAGAATCTGGTGAATGTGGATAGCTTTAATGCATCTGGAGATGGAGTTTCTGATGATACTCAGGCATTTATAAGAGCTTGGACCATGGCCTGCTCTGCACCAAACTCTGTTTTATTGGTTCCACAAGGACGTAGCTATTTAGTAAATGCGACGAAATTCGATGGTCCATGTCAAGAAAAATTGATCATTCAGATTGATGGAACAATCATAGCACCAGATGAGCCTAGCCAATGGGACCCGAAATTCCCAAGGAATTGGCTTCAATTTTCCAAACTCCAAGGAGTTGTATTCCAAGGGAATGGAGTTATAGATGGTTCTGGTACCAAATGGTGGGCTGCTTCTTGCAAGAAGAACAAGTCTAACCCTTGCGTAGGCGCACCAACGGCTCTAACTATATATTCAAGTTCAAATGTGTATGTGAGAGGCTTAACTATCCGGAACAGCCAACAGATGCACTTGATCATACAACGGTCAACTACAGTTCGAATCTCTAGAGTTATGGTCACATCTCCAGGAGACAGTCCCAACACTGATGGAATTCATATAACCGCATCTACCGATGTTGTAGTCCAAGACAGTAAAATCAGCACAGGAGATGATTGTGTCTCGATTGTGAATGGAAGCGCCAAGATAAAGATGAAGAGGATCTACTGTGGACCAGGACATGGAATCAGTATTGGAAGTCTTGGACAAGGACATTCGAAAGGAACCGTCACTGCAGTTGTGTTGGAAACTGCGTTCTTAAAGAACACAACTAATGGTCTAAGAATCAAGACATGGCAGGGTGGTAATGGTTACGTAAAGGGTGTACGTTTCGAGAACGTAGTGATGCAGGACGTTGCGAATCCGATTATAATCGATCAATTCTACTGTGATTCACCATCAACTTGTCAAAACCAGACATCGGCGGTTCACATATCCGAGATAATGTACAGGAACATAACCGGAACCACGAAAAGCTCAAAAGCCATCAACTTCAAGTGTAGTGACGCAGTCCCTTGCAGCCACATTGTCCTCAACAATATCAACTTGGAAGGCAATGATGGAAAAGTAGAAGCATATTGTAACTCTGCTGAAGGGTTTGGTTATGGTGTAGTTCATCCTTCTGCGGATTGTCTGTATTCGCATGACGACAAGAGCTTAAACCAAACTGATCAGTATTTGGCAGAAACTATTATGGTCGAAAAAGAAACTGAAAATGCTCATGATGAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

468

Amino Acids

50.9

Weight (kDa)

5.42

Isoelectric Point (pI)

30.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 99 - 421 5.1e-93 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 260, 1043
Acc65I GGTACC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 509
AccB7I CCANNNNNTGG 2 cut(s) 519, 854
AccIII TCCGGA 1 cut(s) 624
AciI CCGC 3 cut(s) 738, 1097, 1298
AclWI GGATC 1 cut(s) 842
AcsI RAATTY 3 cut(s) 344, 431, 726
AcuI CTGAAG 1 cut(s) 1284
AfaI GTAC 4 cut(s) 120, 511, 999, 1121
AfiI CCNNNNNNNGG 4 cut(s) 519, 560, 854, 1093
AgsI TTSAA 5 cut(s) 79, 452, 591, 597, 1168
AjnI CCWGG 2 cut(s) 700, 847
AloI GAACNNNNNNTCC 2 cut(s) 911, 943
AluBI AGCT 9 cut(s) 47, 59, 103, 213, 266, 324, 1149, 1329, 1402
AluI AGCT 9 cut(s) 47, 59, 103, 213, 266, 324, 1149, 1329, 1402
Alw26I GTCTC 2 cut(s) 699, 799
AlwI GGATC 1 cut(s) 842
Aor13HI TCCGGA 1 cut(s) 624
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 2 cut(s) 524, 1191
ApoI RAATTY 3 cut(s) 344, 431, 726
Asp718I GGTACC 1 cut(s) 509
AspLEI GCGC 2 cut(s) 566, 815
AspS9I GGNCC 4 cut(s) 270, 353, 424, 845
AsuHPI GGTGA 2 cut(s) 211, 1059
AsuII TTCGAA 2 cut(s) 673, 890
AvaII GGWCC 4 cut(s) 270, 353, 424, 845
AxyI CCTNAGG 1 cut(s) 123
BanI GGYRCC 1 cut(s) 509
BarI GAAGNNNNNNTAC 2 cut(s) 821, 853
BbvI GCAGC 2 cut(s) 511, 1203
BccI CCATC 9 cut(s) 34, 224, 344, 380, 494, 716, 1078, 1166, 1226
BceAI ACGGC 1 cut(s) 588
BciT130I CCWGG 2 cut(s) 702, 849
BclI TGATCA 3 cut(s) 372, 648, 1342
BcoDI GTCTC 2 cut(s) 699, 799
BfaI CTAG 2 cut(s) 414, 681
BfmI CTRYAG 3 cut(s) 60, 666, 906
BfoI RGCGCY 1 cut(s) 816
BisI GCNGC 2 cut(s) 525, 1192
BlsI GCNGC 2 cut(s) 526, 1193
Bme1390I CCNGG 2 cut(s) 702, 849
Bme18I GGWCC 4 cut(s) 270, 353, 424, 845
BmgT120I GGNCC 4 cut(s) 270, 353, 424, 845
BmiI GGNNCC 6 cut(s) 309, 425, 426, 511, 898, 1138
BmrFI CCNGG 2 cut(s) 702, 849
BmsI GCATC 4 cut(s) 230, 630, 749, 1008
BpmI CTGGAG 2 cut(s) 246, 684
Bpu14I TTCGAA 2 cut(s) 673, 890
BpuEI CTTGAG 1 cut(s) 125
Bsa29I ATCGAT 1 cut(s) 1047
BsaAI YACGTR 1 cut(s) 988
BsaBI GATNNNNATC 1 cut(s) 1043
BsaJI CCNNGG 4 cut(s) 273, 437, 466, 481
BsaWI WCCGGW 2 cut(s) 624, 1133
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 4 cut(s) 519, 560, 854, 1093
Bse21I CCTNAGG 1 cut(s) 123
Bse3DI GCAATG 1 cut(s) 1234
Bse8I GATNNNNATC 1 cut(s) 1043
BseAI TCCGGA 1 cut(s) 624
BseBI CCWGG 2 cut(s) 702, 849
BseCI ATCGAT 1 cut(s) 1047
BseDI CCNNGG 4 cut(s) 273, 437, 466, 481
BseGI GGATG 3 cut(s) 133, 145, 1288
BseJI GATNNNNATC 1 cut(s) 1043
BseLI CCNNNNNNNGG 4 cut(s) 519, 560, 854, 1093
BseMI GCAATG 1 cut(s) 1234
BseMII CTCAG 2 cut(s) 114, 263
BseXI GCAGC 2 cut(s) 511, 1203
BsgI GTGCAG 1 cut(s) 270
BshFI GGCC 1 cut(s) 278
BshNI GGYRCC 1 cut(s) 509
BshVI ATCGAT 1 cut(s) 1047
BsiSI CCGG 2 cut(s) 625, 1134
BslFI GGGAC 3 cut(s) 437, 696, 1169
BslI CCNNNNNNNGG 4 cut(s) 519, 560, 854, 1093
BsmAI GTCTC 2 cut(s) 699, 799
BsmFI GGGAC 3 cut(s) 437, 696, 1169
BsnI GGCC 1 cut(s) 278
Bsp119I TTCGAA 2 cut(s) 673, 890
Bsp13I TCCGGA 1 cut(s) 624
Bsp1407I TGTACA 1 cut(s) 1119
Bsp143I GATC 5 cut(s) 372, 648, 834, 1048, 1342
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 3 cut(s) 738, 1097, 1298
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 2 cut(s) 115, 262
BspDI ATCGAT 1 cut(s) 1047
BspEI TCCGGA 1 cut(s) 624
BspHI TCATGA 1 cut(s) 1393
BspLI GGNNCC 6 cut(s) 309, 425, 426, 511, 898, 1138
BspMAI CTGCAG 1 cut(s) 910
BspPI GGATC 1 cut(s) 842
BspT104I TTCGAA 2 cut(s) 673, 890
BspT107I GGYRCC 1 cut(s) 509
BsrDI GCAATG 1 cut(s) 1234
BsrGI TGTACA 1 cut(s) 1119
BssECI CCNNGG 4 cut(s) 273, 437, 466, 481
BssMI GATC 5 cut(s) 372, 648, 834, 1048, 1342
BssT1I CCWWGG 4 cut(s) 273, 437, 466, 481
Bst2UI CCWGG 2 cut(s) 702, 849
Bst4CI ACNGT 7 cut(s) 660, 670, 710, 767, 842, 901, 1061
Bst6I CTCTTC 1 cut(s) 824
BstAUI TGTACA 1 cut(s) 1119
BstBAI YACGTR 1 cut(s) 988
BstBI TTCGAA 2 cut(s) 673, 890
BstC8I GCNNGC 1 cut(s) 280
BstDEI CTNAG 3 cut(s) 123, 249, 953
BstDSI CCRYGG 1 cut(s) 273
BstENI CCTNNNNNAGG 1 cut(s) 558
BstF5I GGATG 3 cut(s) 133, 145, 1288
BstH2I RGCGCY 1 cut(s) 816
BstHHI GCGC 2 cut(s) 566, 815
BstKTI GATC 5 cut(s) 375, 651, 837, 1051, 1345
BstMAI GTCTC 2 cut(s) 699, 799
BstMBI GATC 5 cut(s) 372, 648, 834, 1048, 1342
BstMWI GCNNNNNNNGC 2 cut(s) 284, 572
BstNI CCWGG 2 cut(s) 702, 849
BstSCI CCNGG 2 cut(s) 700, 847
BstSFI CTRYAG 3 cut(s) 60, 666, 906
BstSNI TACGTA 1 cut(s) 988
BstV1I GCAGC 2 cut(s) 511, 1203
BstX2I RGATCY 1 cut(s) 834
BstXI CCANNNNNNTGG 3 cut(s) 185, 444, 488
BstYI RGATCY 1 cut(s) 834
Bsu15I ATCGAT 1 cut(s) 1047
Bsu36I CCTNAGG 1 cut(s) 123
BsuRI GGCC 1 cut(s) 278
BsuTUI ATCGAT 1 cut(s) 1047
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 3 cut(s) 133, 145, 1288
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 2 cut(s) 717, 903
Cac8I GCNNGC 1 cut(s) 280
CciI TCATGA 1 cut(s) 1393
CfoI GCGC 2 cut(s) 566, 815
Cfr13I GGNCC 4 cut(s) 270, 353, 424, 845
ClaI ATCGAT 1 cut(s) 1047
CseI GACGC 1 cut(s) 1187
Csp6I GTAC 4 cut(s) 119, 510, 998, 1120
CviAII CATG 6 cut(s) 274, 357, 854, 966, 1316, 1394
CviQI GTAC 4 cut(s) 119, 510, 998, 1120
DdeI CTNAG 3 cut(s) 123, 249, 953
DpnI GATC 5 cut(s) 374, 650, 836, 1050, 1344
DpnII GATC 5 cut(s) 372, 648, 834, 1048, 1342
Eam1104I CTCTTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 824
Eco105I TACGTA 1 cut(s) 988
Eco130I CCWWGG 4 cut(s) 273, 437, 466, 481
Eco47I GGWCC 4 cut(s) 270, 353, 424, 845
Eco57I CTGAAG 1 cut(s) 1284
Eco81I CCTNAGG 1 cut(s) 123
EcoNI CCTNNNNNAGG 1 cut(s) 558
EcoO109I RGGNCCY 1 cut(s) 424
EcoRI GAATTC 1 cut(s) 726
EcoRII CCWGG 2 cut(s) 700, 847
EcoT14I CCWWGG 4 cut(s) 273, 437, 466, 481
EcoT22I ATGCAT 1 cut(s) 223
ErhI CCWWGG 4 cut(s) 273, 437, 466, 481
FaeI CATG 6 cut(s) 277, 360, 857, 969, 1319, 1397
FaqI GGGAC 3 cut(s) 437, 696, 1169
FatI CATG 6 cut(s) 273, 356, 853, 965, 1315, 1393
FbaI TGATCA 3 cut(s) 372, 648, 1342
Fnu4HI GCNGC 2 cut(s) 525, 1192
FokI GGATG 3 cut(s) 140, 152, 1275
Fsp4HI GCNGC 2 cut(s) 525, 1192
FspBI CTAG 2 cut(s) 414, 681
GlaI GCGC 2 cut(s) 565, 814
GluI GCNGC 2 cut(s) 525, 1192
GsuI CTGGAG 2 cut(s) 246, 684
HaeII RGCGCY 1 cut(s) 816
HaeIII GGCC 1 cut(s) 278
HapII CCGG 2 cut(s) 625, 1134
HgaI GACGC 1 cut(s) 1187
HhaI GCGC 2 cut(s) 566, 815
Hin1II CATG 6 cut(s) 277, 360, 857, 969, 1319, 1397
Hin6I GCGC 2 cut(s) 564, 813
HinP1I GCGC 2 cut(s) 564, 813
HincII GTYRAC 1 cut(s) 663
HindII GTYRAC 1 cut(s) 663
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 6 cut(s) 193, 675, 858, 957, 1033, 1064
HpaII CCGG 2 cut(s) 625, 1134
HphI GGTGA 2 cut(s) 211, 1059
Hpy166II GTNNAC 5 cut(s) 171, 663, 845, 998, 1102
Hpy188I TCNGA 4 cut(s) 241, 381, 1038, 1111
Hpy188III TCNNGA 8 cut(s) 225, 362, 625, 681, 796, 961, 1006, 1394
Hpy8I GTNNAC 5 cut(s) 171, 663, 845, 998, 1102
Hpy99I CGWCG 2 cut(s) 169, 343
HpyAV CCTTC 4 cut(s) 160, 1217, 1259, 1302
HpyCH4III ACNGT 7 cut(s) 660, 670, 710, 767, 842, 901, 1061
HpyCH4IV ACGT 5 cut(s) 319, 987, 1000, 1011, 1026
HpyCH4V TGCA 7 cut(s) 221, 287, 534, 643, 908, 1021, 1191
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 572
HpyF3I CTNAG 3 cut(s) 123, 249, 953
HpySE526I ACGT 5 cut(s) 319, 987, 1000, 1011, 1026
Hsp92II CATG 6 cut(s) 277, 360, 857, 969, 1319, 1397
HspAI GCGC 2 cut(s) 564, 813
KflI GGGWCCC 1 cut(s) 424
Kpn2I TCCGGA 1 cut(s) 624
KpnI GGTACC 1 cut(s) 513
Ksp22I TGATCA 3 cut(s) 372, 648, 1342
Kzo9I GATC 5 cut(s) 372, 648, 834, 1048, 1342
LmnI GCTCC 1 cut(s) 56
Lsp1109I GCAGC 2 cut(s) 511, 1203
LweI GCATC 4 cut(s) 230, 630, 749, 1008
MaeI CTAG 2 cut(s) 414, 681
MaeII ACGT 5 cut(s) 319, 987, 1000, 1011, 1026
MaeIII GTNAC 6 cut(s) 145, 691, 901, 983, 1175, 1253
MalI GATC 5 cut(s) 374, 650, 836, 1050, 1344
MboI GATC 5 cut(s) 372, 648, 834, 1048, 1342
MboII GAAGA 2 cut(s) 550, 841
MflI RGATCY 1 cut(s) 834
MluCI AATT 7 cut(s) 344, 368, 431, 442, 452, 726, 1052
MmeI TCCRAC 1 cut(s) 897
MnlI CCTC 6 cut(s) 118, 122, 130, 605, 825, 1214
Mph1103I ATGCAT 1 cut(s) 223
MroI TCCGGA 1 cut(s) 624
MseI TTAA 6 cut(s) 6, 216, 617, 932, 1331, 1405
MspI CCGG 2 cut(s) 625, 1134
MspR9I CCNGG 2 cut(s) 702, 849
MvaI CCWGG 2 cut(s) 702, 849
MwoI GCNNNNNNNGC 2 cut(s) 284, 572
NcoI CCATGG 1 cut(s) 273
NdeII GATC 5 cut(s) 372, 648, 834, 1048, 1342
NlaIII CATG 6 cut(s) 277, 360, 857, 969, 1319, 1397
NlaIV GGNNCC 6 cut(s) 309, 425, 426, 511, 898, 1138
NmuCI GTSAC 4 cut(s) 145, 691, 901, 1175
NsiI ATGCAT 1 cut(s) 223
NspV TTCGAA 2 cut(s) 673, 890
PagI TCATGA 1 cut(s) 1393
PfeI GAWTC 6 cut(s) 193, 675, 858, 957, 1033, 1064
PflFI GACNNNGTC 1 cut(s) 1181
PflMI CCANNNNNTGG 2 cut(s) 519, 854
PfoI TCCNGGA 1 cut(s) 700
PkrI GCNGC 2 cut(s) 526, 1193
Ppu21I YACGTR 1 cut(s) 988
PpuMI RGGWCCY 1 cut(s) 424
PsiI TTATAA 2 cut(s) 260, 1043
Psp5II RGGWCCY 1 cut(s) 424
Psp6I CCWGG 2 cut(s) 700, 847
PspGI CCWGG 2 cut(s) 700, 847
PspN4I GGNNCC 6 cut(s) 309, 425, 426, 511, 898, 1138
PspPI GGNCC 4 cut(s) 270, 353, 424, 845
PspPPI RGGWCCY 1 cut(s) 424
PstI CTGCAG 1 cut(s) 910
PsuI RGATCY 1 cut(s) 834
PsyI GACNNNGTC 1 cut(s) 1181
RsaI GTAC 4 cut(s) 120, 511, 999, 1121
RsaNI GTAC 4 cut(s) 119, 510, 998, 1120
SaqAI TTAA 6 cut(s) 6, 216, 617, 932, 1331, 1405
SatI GCNGC 2 cut(s) 525, 1192
Sau3AI GATC 5 cut(s) 372, 648, 834, 1048, 1342
Sau96I GGNCC 4 cut(s) 270, 353, 424, 845
ScrFI CCNGG 2 cut(s) 702, 849
SfaNI GCATC 4 cut(s) 230, 630, 749, 1008
SfcI CTRYAG 3 cut(s) 60, 666, 906
SfuI TTCGAA 2 cut(s) 673, 890
SinI GGWCC 4 cut(s) 270, 353, 424, 845
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SnaBI TACGTA 1 cut(s) 988
Sse9I AATT 7 cut(s) 344, 368, 431, 442, 452, 726, 1052
SsiI CCGC 3 cut(s) 738, 1097, 1298
SspI AATATT 1 cut(s) 182
SspMI CTAG 2 cut(s) 414, 681
StyD4I CCNGG 2 cut(s) 700, 847
StyI CCWWGG 4 cut(s) 273, 437, 466, 481
TaaI ACNGT 7 cut(s) 660, 670, 710, 767, 842, 901, 1061
TaiI ACGT 5 cut(s) 322, 990, 1003, 1014, 1029
TaqI TCGA 8 cut(s) 114, 348, 673, 797, 890, 1005, 1047, 1371
TasI AATT 7 cut(s) 344, 368, 431, 442, 452, 726, 1052
TatI WGTACW 1 cut(s) 1119
TfiI GAWTC 6 cut(s) 193, 675, 858, 957, 1033, 1064
Tru1I TTAA 6 cut(s) 6, 216, 617, 932, 1331, 1405
Tru9I TTAA 6 cut(s) 6, 216, 617, 932, 1331, 1405
TscAI CASTG 2 cut(s) 724, 910
TseFI GTSAC 4 cut(s) 145, 691, 901, 1175
TseI GCWGC 2 cut(s) 524, 1191
Tsp45I GTSAC 4 cut(s) 145, 691, 901, 1175
TspDTI ATGAA 3 cut(s) 719, 842, 1277
TspRI CASTG 2 cut(s) 724, 910
Tth111I GACNNNGTC 1 cut(s) 1181
Van91I CCANNNNNTGG 2 cut(s) 519, 854
VpaK11BI GGWCC 4 cut(s) 270, 353, 424, 845
XagI CCTNNNNNAGG 1 cut(s) 558
XapI RAATTY 3 cut(s) 344, 431, 726
XbaI TCTAGA 1 cut(s) 680
XspI CTAG 2 cut(s) 414, 681
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.