MD16G1095500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004713
Physical Location & Seq
Reverse (-)
6606828 .. 6612450
5623 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1095500.v1.1.491

Sequence Viewer

Length: 3399 bp
ATGTTGTCAGTTTCACTTCGGAGAGGCGCATGGCCAATGCGGATTACTAGGTTACTGTCTTCCGTACGTTTCAGTACAAACTTCAACGTAGCCACAGCTTCATCAACCTCGTCATCTCTGCGTTTGAACTCGTACTTTGAACCCAGTGTTCGTGACCTTGTATACTCCATCAACCGAAAAATCGATGGTTTGATTAAATCCGGGGACTTGAACACTGCCCTCAAACTGTTCGATGAAATGCCTGTATACGATGTTGTAACATATAACATGCTGATTTCTGGGCACGGCCGACATGGGTTTCCGGAACAGGCTTTTCACTTCTATGCTGAAATGGTTTCACAAGGAATTAGAGAAAGTGCTTCGACGTTTTCGTCGGTTTTGGGTGTTTGTAACGACGCAGGGTTTTGTAGAGAAGGAATGCAAGTTCATTGCAGGGTTGTCTCACTTGGGTTCGGCTTGAATTTGTATGTGGGGGGCTCCCTTGTGGATCTTTATTTGCATATGGGACTAGATAATGTAGCTTTGAAGCTGTTTGATGAATTGCCGTACCGAAATGTAGCTGTTTGGAATTTGTTGTTACGTGGGTTTTGTGAACTTGGTCGGTTGGATGAGCTATGTGGTGTGTTTTCCACGATGGAATTTGATGGTGTGGATCCAAATGGGCTTACTTATTGTTATTTGATCCGCGGATGTAGTAATGGGAGGCTCTTGGATGAAGGAAAGCAGCTACATTGCCTAGTTGTTAAGGACGGATGGGTGGAATCGAATGTATTTGTTGCGAATGCTCTGGTGGATTTGTACTCTGCTTGTGGGAGCTTGATTGATGCAACAGAAGCATTTGAAGCTATTCAAATGGAGGATGTGATTTCGTGGAATTCAATTATCTGGGTCTGCGCAGAAAATGGTTTGCTGCTTGATGCTCTTAAGTTATTTGTTGAGATGCAGTTTTGGGAGAAGAGGCCATCGATTCGTTCGTTTATAGGGTTTTTGAATTTAGCGAGTAGGACTGAGAATATTGAACTTGGAAAGCAGATGCACTCTTATGTTTTGAAGTCGGGTTTTGGTCATGGTGGGAGTGTTCATGTTCAATCTGCTCTAATAGATATGTACGGGAAATGCGTCGACATTGAAAGTTCGGTGTCCATTTATGAAAGTGTTGGTGAGAAAACGTTGGAGTCTTGTAATTCTTTGATGACCTCTTTGTTGCATTGCGGTGTTATTGAGGATGTGGTGGAAATGTTTGGTTTGATGGTTGATAAGGGAATTGGTCTTGATGAAGTTACTCTATCCACGACCCTGAAAGCCTTATCGATATCTGCTTTAGCAAGTTCGGGCAGCTGCAAATTGGTACATTGTTGTGCAATAAAATCAGGTTTTGGATCTGATATTGCGGTTTCGTGTTCACTAATCGATGCCTATGCAAGGTGTGGCCATGTCAAGCTTTCTCGACAAGTTTTCGAACAACTACCTTCACCGAATGTCATTTGTTTTACGTCGTTAATCCATGGATATGCACGAAATGGAACGGGAAGTGAAGGTCTTGATTTGCTTCAAGAAATGATCTCAAAGGGTCTGAAACCTGATCAAGTGACAATCTTGGGTGTGTTAAGTGGGTGCAACCATTCAGGACTGGTCAAAGAAGCAAGATTTGTGTTTGATTCAATGAAAAAACTATATGATATTTCTCCGGACAGAAAGCATTTTTCGTGTATGGTTGATCTCTTAGGCCGGGCGGGTATGCTAGAGGAAGCTGAAGACTTGCTGCAGCAGGCACCGGTTAATGGGGACTGTGTGATGTGGAGCTCGTTGTTGCGTAGTTGTAGGATACACAAGAACGAAACAGTCGGAAGACGAACGGTAAAAACTTTGTTGTTGGAGCTTGATGTCGAAGATCCTGAGATATGGTTGCAAGCTTCAAACTTTTATTCTGAAATTGGAGAGTTTGACACTGCAATGCAAATCAGGGAAATTGCAATAGCAAGGAAAGTGAAATGGGAGATGGGTCACAGTTTGATCGAGACAAGCATGGATAATCTCTTTGCGGTTTTTGTTTTTGGTATGCTGATAGCGGTTTTTGGAGTTGCAAGAGACGTGGTCTGTGACAACATGAGCATGCTTCATGAGCTTGAAAGCTTTAACATAGAAGAAGACAATGAGCTGAATGTTTTCGACATCCCGACATGGAAAAGTGAACGTGGTAGCAAGACTCTTGTTAACGTGGATAGCTTCGGCGCAGCTGGGGATGGAGTTTCTGATGATACTCAGGCTTTCCAGAAAGCCTGGGGTATTGCTTGCAATACAACAAAAGCAGTTTTCTTGGTACCCCAAGGACGCCGGTATCTAGTGAATGCTACAAAATTTCAAGGGCCATGTGCGGATAACTTGATCATCCAGATTGAAGGAACTATAGTAGCACCAGATGAGCCTAATAACTGGGATCCAAATTTTCCACGAACATGGCTTGATTTCACTAAACTGAATGGAGTCCTTTTCCAAGGGCATGGCGTTATTGACGGCTCAGGCAGTAAATGGTGGGCCGCATCCTGCAAAACGAACAAGACAAATCCTTGCAGAGGGGCACCAACTGCATTTACTATTGATAAAAGCTCAGCTATAAATGTAAAAGGCATCACCATCAAGAACGCCCAACAGATGAATTTTGTCATCTCTCAGTGTGACGCGGTGCGAGTTAATGCAGTACAAGTCTCGGCTCCAGGAGACAGTCCCAACACTGATGGAATCCATATCACGGCATCTACTAATGTGATTCTCCAAGACTGCAAAATTGGAACAGGTGATGATTGCGTCTCAATTGTCAATGGTACCTCTGGAATTAAGATGAAAAGAATCTTTTGCGGACCAGGACATGGAATCAGCATCGGAAGTCTTGGGAAGGATAACTCCACTGCCATGGTCACAAAAGTGGTCTTGGATACAGCATACCTCAGGGAGACGACCAATGGCCTCAGGATTAAGACTTGGCAGGGCGGTGCTGGTTATGTTCGTGGAGTCCGTTTCCAGAATGTGAAGATGGAAAATGTATCAAACCCGATAATCATAGATCAGTTTTACTGTGATTCCCCCAAAACTTGTCAAAATCTGACATCAGCGGTGCAAATAACCCAAGTCATGTACAAGAACATTTATGGAACCACAAAGAGTGCGAAGGCCATGAAATTTGCCTGCAGTGACACAGTTCCATGCAGGAACATAGTCCTGACTGACGTCAACTTAGAGAAGGAGGATGGCCAAGTGGAAACATACTGCAACTCTGCGCAAGGCTTCGGGTATGGCATTGTGCATCCTTCAGCAGAATGTCTGAATTCCCATGACAAAGAGACATCTGAAGCTGAGCTTGCAGATCTGACTACAGCGGATATTGTGCACACTGAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 PPR PPR_2

Protein Analysis

1133

Amino Acids

124.11

Weight (kDa)

5.33

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 84 - 130 1.7e-09 PPR repeat family
PPR PF01535 86 - 116 2e-06 PPR repeat
PPR_2 PF13041 184 - 233 1.9e-10 PPR repeat family
PPR_2 PF13041 492 - 539 1.4e-09 PPR repeat family
PPR PF01535 496 - 525 7.3e-06 PPR repeat
Pect-lyase_RHGA_epim PF12708 738 - 783 6.4e-06 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 767 - 1091 5e-91 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 370
AatII GACGTC 1 cut(s) 3228
Acc16I TGCGCA 2 cut(s) 895, 3276
Acc65I GGTACC 2 cut(s) 2320, 2822
AccB1I GGYRCC 4 cut(s) 1771, 2320, 2578, 2822
AccB7I CCANNNNNTGG 1 cut(s) 2867
AccI GTMKAC 3 cut(s) 162, 246, 1122
AccII CGCG 2 cut(s) 687, 2681
AccIII TCCGGA 2 cut(s) 301, 1687
AclI AACGTT 1 cut(s) 1169
AclWI GGATC 8 cut(s) 495, 647, 660, 676, 1387, 1886, 2432, 2445
AcoI YGGCCR 4 cut(s) 32, 286, 1429, 3247
AcuI CTGAAG 3 cut(s) 1773, 3291, 3366
AcyI GRCGYC 2 cut(s) 2332, 3225
AfiI CCNNNNNNNGG 5 cut(s) 1422, 1781, 2573, 2749, 2867
AflII CTTAAG 1 cut(s) 923
AgeI ACCGGT 1 cut(s) 1774
AjiI CACGTC 1 cut(s) 2092
AjnI CCWGG 3 cut(s) 2279, 2713, 2860
AleI CACNNNNGTG 1 cut(s) 2921
AloI GAACNNNNNNTCC 2 cut(s) 408, 440
Alw21I GWGCWC 2 cut(s) 1805, 3387
Alw26I GTCTC 8 cut(s) 445, 2012, 2082, 2710, 2712, 2812, 2945, 3332
Alw44I GTGCAC 1 cut(s) 3383
AlwI GGATC 8 cut(s) 495, 647, 660, 676, 1387, 1886, 2432, 2445
Aor13HI TCCGGA 2 cut(s) 301, 1687
ApaLI GTGCAC 1 cut(s) 3383
ApeKI GCWGC 7 cut(s) 724, 910, 1335, 1338, 1762, 1765, 2234
AsiGI ACCGGT 1 cut(s) 1774
Asp700I GAANNNNTTC 2 cut(s) 846, 2165
Asp718I GGTACC 2 cut(s) 2320, 2822
AspLEI GCGC 4 cut(s) 29, 896, 2234, 3277
AspS9I GGNCC 3 cut(s) 2366, 2535, 2858
AsuC2I CCSGG 2 cut(s) 202, 1730
AsuHPI GGTGA 4 cut(s) 1172, 1464, 2623, 2807
AsuII TTCGAA 1 cut(s) 1458
AvaII GGWCC 1 cut(s) 2858
AxyI CCTNAGG 2 cut(s) 2945, 2966
BaeGI GKGCMC 3 cut(s) 285, 2581, 3387
BaeI ACNNNNGTAYC 2 cut(s) 2321, 2354
BalI TGGCCA 3 cut(s) 34, 1431, 3249
BamHI GGATCC 2 cut(s) 652, 2437
BanI GGYRCC 4 cut(s) 1771, 2320, 2578, 2822
BanII GRGCYC 2 cut(s) 479, 1805
BbsI GAAGAC 4 cut(s) 51, 1761, 1855, 2154
Bbv12I GWGCWC 2 cut(s) 1805, 3387
BbvI GCAGC 7 cut(s) 736, 897, 1325, 1347, 1749, 1777, 2246
BceAI ACGGC 4 cut(s) 301, 529, 2530, 2766
BcgI CGANNNNNNTGC 2 cut(s) 1400, 1434
BciT130I CCWGG 3 cut(s) 2281, 2715, 2862
BciVI GTATCC 2 cut(s) 1818, 2926
BclI TGATCA 2 cut(s) 1582, 2385
BcnI CCSGG 2 cut(s) 202, 1730
BcoDI GTCTC 8 cut(s) 445, 2012, 2082, 2710, 2712, 2812, 2945, 3332
BfaI CTAG 5 cut(s) 48, 509, 737, 1742, 2342
BfmI CTRYAG 4 cut(s) 1763, 2406, 3184, 3369
BfrI CTTAAG 1 cut(s) 923
BfuI GTATCC 2 cut(s) 1818, 2926
BglII AGATCT 1 cut(s) 3361
BisI GCNGC 8 cut(s) 725, 911, 1336, 1339, 1763, 1766, 2235, 2538
BlpI GCTNAGC 2 cut(s) 2608, 3351
BlsI GCNGC 8 cut(s) 726, 912, 1337, 1340, 1764, 1767, 2236, 2539
Bme1390I CCNGG 5 cut(s) 202, 1730, 2281, 2715, 2862
Bme18I GGWCC 1 cut(s) 2858
BmgBI CACGTC 1 cut(s) 2092
BmgT120I GGNCC 3 cut(s) 2366, 2535, 2858
BmiI GGNNCC 9 cut(s) 478, 654, 1773, 2322, 2439, 2580, 2712, 2824, 3151
BmrFI CCNGG 5 cut(s) 202, 1730, 2281, 2715, 2862
BmrI ACTGGG 2 cut(s) 138, 2443
BmuI ACTGGG 2 cut(s) 138, 2443
BoxI GACNNNNGTC 1 cut(s) 3224
BpiI GAAGAC 4 cut(s) 51, 1761, 1855, 2154
BpmI CTGGAG 1 cut(s) 2697
Bpu10I CCTNAGC 1 cut(s) 2518
Bpu1102I GCTNAGC 2 cut(s) 2608, 3351
Bpu14I TTCGAA 1 cut(s) 1458
BpuMI CCSGG 2 cut(s) 202, 1730
Bsa29I ATCGAT 4 cut(s) 183, 965, 1310, 1410
BsaAI YACGTR 1 cut(s) 581
BsaHI GRCGYC 2 cut(s) 2332, 3225
BsaJI CCNNGG 7 cut(s) 201, 685, 1504, 2280, 2326, 2494, 2910
BsaWI WCCGGW 3 cut(s) 301, 1687, 1774
Bsc4I CCNNNNNNNGG 5 cut(s) 1422, 1781, 2573, 2749, 2867
Bse118I RCCGGY 2 cut(s) 1774, 2334
Bse1I ACTGG 3 cut(s) 144, 1635, 2438
Bse21I CCTNAGG 2 cut(s) 2945, 2966
Bse3DI GCAATG 4 cut(s) 427, 730, 1207, 1959
BseAI TCCGGA 2 cut(s) 301, 1687
BseBI CCWGG 3 cut(s) 2281, 2715, 2862
BseCI ATCGAT 4 cut(s) 183, 965, 1310, 1410
BseDI CCNNGG 7 cut(s) 201, 685, 1504, 2280, 2326, 2494, 2910
BseLI CCNNNNNNNGG 5 cut(s) 1422, 1781, 2573, 2749, 2867
BseMI GCAATG 4 cut(s) 427, 730, 1207, 1959
BseNI ACTGG 3 cut(s) 144, 1635, 2438
BseSI GKGCMC 3 cut(s) 285, 2581, 3387
BseX3I CGGCCG 1 cut(s) 286
BseXI GCAGC 7 cut(s) 736, 897, 1325, 1347, 1749, 1777, 2246
BseYI CCCAGC 1 cut(s) 2237
Bsh1236I CGCG 2 cut(s) 687, 2681
Bsh1285I CGRYCG 1 cut(s) 289
BshNI GGYRCC 4 cut(s) 1771, 2320, 2578, 2822
BshTI ACCGGT 1 cut(s) 1774
BshVI ATCGAT 4 cut(s) 183, 965, 1310, 1410
BsiEI CGRYCG 1 cut(s) 289
BsiHKAI GWGCWC 2 cut(s) 1805, 3387
BsiSI CCGG 6 cut(s) 201, 302, 1688, 1729, 1775, 2335
BsiWI CGTACG 1 cut(s) 64
BslFI GGGAC 4 cut(s) 218, 519, 1799, 2709
BslI CCNNNNNNNGG 5 cut(s) 1422, 1781, 2573, 2749, 2867
BsmAI GTCTC 8 cut(s) 445, 2012, 2082, 2710, 2712, 2812, 2945, 3332
BsmBI CGTCTC 3 cut(s) 2082, 2812, 2945
BsmFI GGGAC 4 cut(s) 218, 519, 1799, 2709
BsmI GAATGC 3 cut(s) 423, 787, 2353
Bsp119I TTCGAA 1 cut(s) 1458
Bsp1286I GDGCHC 5 cut(s) 285, 479, 1805, 2581, 3387
Bsp13I TCCGGA 2 cut(s) 301, 1687
Bsp1407I TGTACA 1 cut(s) 3132
Bsp1720I GCTNAGC 2 cut(s) 2608, 3351
Bsp19I CCATGG 2 cut(s) 1504, 2910
BspDI ATCGAT 4 cut(s) 183, 965, 1310, 1410
BspEI TCCGGA 2 cut(s) 301, 1687
BspFNI CGCG 2 cut(s) 687, 2681
BspHI TCATGA 1 cut(s) 2119
BspLI GGNNCC 9 cut(s) 478, 654, 1773, 2322, 2439, 2580, 2712, 2824, 3151
BspMAI CTGCAG 2 cut(s) 1767, 3188
BspPI GGATC 8 cut(s) 495, 647, 660, 676, 1387, 1886, 2432, 2445
BspT104I TTCGAA 1 cut(s) 1458
BspT107I GGYRCC 4 cut(s) 1771, 2320, 2578, 2822
BspTI CTTAAG 1 cut(s) 923
BsrDI GCAATG 4 cut(s) 427, 730, 1207, 1959
BsrFI RCCGGY 2 cut(s) 1774, 2334
BsrGI TGTACA 1 cut(s) 3132
BsrI ACTGG 3 cut(s) 144, 1635, 2438
BssAI RCCGGY 2 cut(s) 1774, 2334
BssECI CCNNGG 7 cut(s) 201, 685, 1504, 2280, 2326, 2494, 2910
BssNAI GTATAC 2 cut(s) 163, 247
BssNI GRCGYC 2 cut(s) 2332, 3225
BssT1I CCWWGG 4 cut(s) 1504, 2326, 2494, 2910
Bst1107I GTATAC 2 cut(s) 163, 247
Bst2UI CCWGG 3 cut(s) 2281, 2715, 2862
Bst4CI ACNGT 9 cut(s) 57, 228, 1790, 1843, 1858, 2009, 2723, 3074, 3196
Bst6I CTCTTC 1 cut(s) 950
BstACI GRCGYC 2 cut(s) 2332, 3225
BstAFI CTTAAG 1 cut(s) 923
BstAPI GCANNNNNTGC 1 cut(s) 2585
BstAUI TGTACA 1 cut(s) 3132
BstBAI YACGTR 1 cut(s) 581
BstBI TTCGAA 1 cut(s) 1458
BstC8I GCNNGC 6 cut(s) 1770, 1911, 2114, 2293, 3184, 3357
BstDSI CCRYGG 3 cut(s) 685, 1504, 2910
BstENI CCTNNNNNAGG 2 cut(s) 1420, 2571
BstFNI CGCG 2 cut(s) 687, 2681
BstHHI GCGC 4 cut(s) 29, 896, 2234, 3277
BstMAI GTCTC 8 cut(s) 445, 2012, 2082, 2710, 2712, 2812, 2945, 3332
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 6 cut(s) 833, 842, 2122, 2585, 3356, 3391
BstNI CCWGG 3 cut(s) 2281, 2715, 2862
BstNSI RCATGY 2 cut(s) 271, 2116
BstPAI GACNNNNGTC 1 cut(s) 3224
BstSCI CCNGG 5 cut(s) 200, 1728, 2279, 2713, 2860
BstSFI CTRYAG 4 cut(s) 1763, 2406, 3184, 3369
BstSLI GKGCMC 3 cut(s) 285, 2581, 3387
BstUI CGCG 2 cut(s) 687, 2681
BstV1I GCAGC 7 cut(s) 736, 897, 1325, 1347, 1749, 1777, 2246
BstV2I GAAGAC 4 cut(s) 51, 1761, 1855, 2154
BstX2I RGATCY 6 cut(s) 487, 652, 1379, 1891, 2437, 3361
BstXI CCANNNNNNTGG 3 cut(s) 2457, 2501, 2911
BstYI RGATCY 6 cut(s) 487, 652, 1379, 1891, 2437, 3361
BstZ17I GTATAC 2 cut(s) 163, 247
BstZI CGGCCG 1 cut(s) 286
Bsu15I ATCGAT 4 cut(s) 183, 965, 1310, 1410
Bsu36I CCTNAGG 2 cut(s) 2945, 2966
BsuI GTATCC 2 cut(s) 1818, 2926
BsuTUI ATCGAT 4 cut(s) 183, 965, 1310, 1410
BtgI CCRYGG 3 cut(s) 685, 1504, 2910
BtrI CACGTC 1 cut(s) 2092
BtsI GCAGTG 4 cut(s) 213, 1947, 2904, 3193
BtsIMutI CAGTG 8 cut(s) 151, 213, 1947, 2678, 2730, 2904, 3193, 3387
Cac8I GCNNGC 6 cut(s) 1770, 1911, 2114, 2293, 3184, 3357
CciI TCATGA 1 cut(s) 2119
CfoI GCGC 4 cut(s) 29, 896, 2234, 3277
Cfr10I RCCGGY 2 cut(s) 1774, 2334
Cfr13I GGNCC 3 cut(s) 2366, 2535, 2858
Cfr42I CCGCGG 1 cut(s) 688
ClaI ATCGAT 4 cut(s) 183, 965, 1310, 1410
CseI GACGC 5 cut(s) 404, 1108, 2340, 2687, 2794
CspAI ACCGGT 1 cut(s) 1774
CspCI CAANNNNNGTGG 2 cut(s) 2073, 2108
DrdI GACNNNNNNGTC 1 cut(s) 370
DseDI GACNNNNNNGTC 1 cut(s) 370
EaeI YGGCCR 4 cut(s) 32, 286, 1429, 3247
EagI CGGCCG 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 950
EarI CTCTTC 1 cut(s) 950
Ecl136II GAGCTC 1 cut(s) 1803
EclXI CGGCCG 1 cut(s) 286
Eco130I CCWWGG 4 cut(s) 1504, 2326, 2494, 2910
Eco24I GRGCYC 2 cut(s) 479, 1805
Eco32I GATATC 1 cut(s) 1314
Eco47I GGWCC 1 cut(s) 2858
Eco52I CGGCCG 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 1803
Eco57I CTGAAG 3 cut(s) 1773, 3291, 3366
Eco81I CCTNAGG 2 cut(s) 2945, 2966
EcoICRI GAGCTC 1 cut(s) 1803
EcoNI CCTNNNNNAGG 2 cut(s) 1420, 2571
EcoRI GAATTC 2 cut(s) 874, 3322
EcoRII CCWGG 3 cut(s) 2279, 2713, 2860
EcoRV GATATC 1 cut(s) 1314
EcoT14I CCWWGG 4 cut(s) 1504, 2326, 2494, 2910
EcoT38I GRGCYC 2 cut(s) 479, 1805
ErhI CCWWGG 4 cut(s) 1504, 2326, 2494, 2910
Esp3I CGTCTC 3 cut(s) 2082, 2812, 2945
FalI AAGNNNNNCTT 2 cut(s) 3339, 3371
FaqI GGGAC 4 cut(s) 218, 519, 1799, 2709
FauI CCCGC 1 cut(s) 1726
FauNDI CATATG 1 cut(s) 501
FbaI TGATCA 2 cut(s) 1582, 2385
FblI GTMKAC 3 cut(s) 162, 246, 1122
Fnu4HI GCNGC 8 cut(s) 725, 911, 1336, 1339, 1763, 1766, 2235, 2538
FriOI GRGCYC 2 cut(s) 479, 1805
Fsp4HI GCNGC 8 cut(s) 725, 911, 1336, 1339, 1763, 1766, 2235, 2538
FspBI CTAG 5 cut(s) 48, 509, 737, 1742, 2342
FspI TGCGCA 2 cut(s) 895, 3276
GlaI GCGC 4 cut(s) 28, 895, 2233, 3276
GluI GCNGC 8 cut(s) 725, 911, 1336, 1339, 1763, 1766, 2235, 2538
GsaI CCCAGC 1 cut(s) 2241
GsuI CTGGAG 1 cut(s) 2697
HapII CCGG 6 cut(s) 201, 302, 1688, 1729, 1775, 2335
HgaI GACGC 5 cut(s) 404, 1108, 2340, 2687, 2794
HhaI GCGC 4 cut(s) 29, 896, 2234, 3277
Hin1I GRCGYC 2 cut(s) 2332, 3225
Hin6I GCGC 4 cut(s) 27, 894, 2232, 3275
HinP1I GCGC 4 cut(s) 27, 894, 2232, 3275
HincII GTYRAC 3 cut(s) 1123, 2215, 3229
HindII GTYRAC 3 cut(s) 1123, 2215, 3229
HindIII AAGCTT 3 cut(s) 1439, 1911, 2131
HpaI GTTAAC 1 cut(s) 2215
HpaII CCGG 6 cut(s) 201, 302, 1688, 1729, 1775, 2335
HphI GGTGA 4 cut(s) 1172, 1464, 2623, 2807
Hpy166II GTNNAC 9 cut(s) 163, 247, 593, 1123, 1403, 2192, 2215, 3229, 3385
Hpy8I GTNNAC 9 cut(s) 163, 247, 593, 1123, 1403, 2192, 2215, 3229, 3385
Hpy99I CGWCG 5 cut(s) 367, 376, 398, 1124, 1498
HpyAV CCTTC 9 cut(s) 407, 710, 1479, 1529, 2393, 2887, 3160, 3232, 3315
HpyCH4III ACNGT 9 cut(s) 57, 228, 1790, 1843, 1858, 2009, 2723, 3074, 3196
HpyF10VI GCNNNNNNNGC 6 cut(s) 833, 842, 2122, 2585, 3356, 3391
Hsp92I GRCGYC 2 cut(s) 2332, 3225
HspAI GCGC 4 cut(s) 27, 894, 2232, 3275
Kpn2I TCCGGA 2 cut(s) 301, 1687
KpnI GGTACC 2 cut(s) 2324, 2826
Ksp22I TGATCA 2 cut(s) 1582, 2385
KspAI GTTAAC 1 cut(s) 2215
KspI CCGCGG 1 cut(s) 688
LmnI GCTCC 5 cut(s) 482, 813, 1800, 1876, 2716
Lsp1109I GCAGC 7 cut(s) 736, 897, 1325, 1347, 1749, 1777, 2246
MaeI CTAG 5 cut(s) 48, 509, 737, 1742, 2342
MboII GAAGA 8 cut(s) 51, 967, 1766, 1860, 1901, 2156, 2159, 3040
MfeI CAATTG 1 cut(s) 2811
MflI RGATCY 6 cut(s) 487, 652, 1379, 1891, 2437, 3361
MhlI GDGCHC 5 cut(s) 285, 479, 1805, 2581, 3387
MlsI TGGCCA 3 cut(s) 34, 1431, 3249
MluNI TGGCCA 3 cut(s) 34, 1431, 3249
MlyI GAGTC 4 cut(s) 1184, 2200, 2493, 3018
MmeI TCCRAC 4 cut(s) 585, 1152, 1825, 1854
Mox20I TGGCCA 3 cut(s) 34, 1431, 3249
MroI TCCGGA 2 cut(s) 301, 1687
MroXI GAANNNNTTC 2 cut(s) 846, 2165
MscI TGGCCA 3 cut(s) 34, 1431, 3249
Msp20I TGGCCA 3 cut(s) 34, 1431, 3249
MspA1I CMGCKG 5 cut(s) 687, 1338, 2237, 3110, 3374
MspCI CTTAAG 1 cut(s) 923
MspI CCGG 6 cut(s) 201, 302, 1688, 1729, 1775, 2335
MspR9I CCNGG 5 cut(s) 202, 1730, 2281, 2715, 2862
MunI CAATTG 1 cut(s) 2811
Mva1269I GAATGC 3 cut(s) 423, 787, 2353
MvaI CCWGG 3 cut(s) 2281, 2715, 2862
MvnI CGCG 2 cut(s) 687, 2681
MwoI GCNNNNNNNGC 6 cut(s) 833, 842, 2122, 2585, 3356, 3391
NciI CCSGG 2 cut(s) 202, 1730
NcoI CCATGG 2 cut(s) 1504, 2910
NdeI CATATG 1 cut(s) 501
NlaIV GGNNCC 9 cut(s) 478, 654, 1773, 2322, 2439, 2580, 2712, 2824, 3151
NmeAIII GCCGAG 1 cut(s) 2687
NmuCI GTSAC 7 cut(s) 152, 1588, 2003, 2099, 2675, 2914, 3188
NsbI TGCGCA 2 cut(s) 895, 3276
NspI RCATGY 2 cut(s) 271, 2116
NspV TTCGAA 1 cut(s) 1458
OliI CACNNNNGTG 1 cut(s) 2921
PaeI GCATGC 1 cut(s) 2116
PagI TCATGA 1 cut(s) 2119
PcsI WCGNNNNNNNCGW 4 cut(s) 368, 971, 1116, 3010
PctI GAATGC 3 cut(s) 423, 787, 2353
PdmI GAANNNNTTC 2 cut(s) 846, 2165
PfeI GAWTC 8 cut(s) 761, 967, 1658, 2739, 2767, 2847, 2871, 3077
Pfl23II CGTACG 1 cut(s) 64
PflFI GACNNNGTC 1 cut(s) 2093
PflMI CCANNNNNTGG 1 cut(s) 2867
PfoI TCCNGGA 1 cut(s) 2713
PinAI ACCGGT 1 cut(s) 1774
PkrI GCNGC 8 cut(s) 726, 912, 1337, 1340, 1764, 1767, 2236, 2539
PleI GAGTC 4 cut(s) 1183, 2200, 2492, 3017
PpsI GAGTC 4 cut(s) 1183, 2200, 2492, 3017
Ppu21I YACGTR 1 cut(s) 581
PshAI GACNNNNGTC 1 cut(s) 3224
Psp124BI GAGCTC 1 cut(s) 1805
Psp1406I AACGTT 1 cut(s) 1169
Psp6I CCWGG 3 cut(s) 2279, 2713, 2860
PspFI CCCAGC 1 cut(s) 2237
PspGI CCWGG 3 cut(s) 2279, 2713, 2860
PspLI CGTACG 1 cut(s) 64
PspN4I GGNNCC 9 cut(s) 478, 654, 1773, 2322, 2439, 2580, 2712, 2824, 3151
PspPI GGNCC 3 cut(s) 2366, 2535, 2858
PstI CTGCAG 2 cut(s) 1767, 3188
PsuI RGATCY 6 cut(s) 487, 652, 1379, 1891, 2437, 3361
PsyI GACNNNGTC 1 cut(s) 2093
PvuII CAGCTG 2 cut(s) 1338, 2237
SacI GAGCTC 1 cut(s) 1805
SacII CCGCGG 1 cut(s) 688
SalI GTCGAC 1 cut(s) 1121
SatI GCNGC 8 cut(s) 725, 911, 1336, 1339, 1763, 1766, 2235, 2538
Sau96I GGNCC 3 cut(s) 2366, 2535, 2858
SchI GAGTC 4 cut(s) 1184, 2200, 2493, 3018
ScrFI CCNGG 5 cut(s) 202, 1730, 2281, 2715, 2862
SduI GDGCHC 5 cut(s) 285, 479, 1805, 2581, 3387
SfcI CTRYAG 4 cut(s) 1763, 2406, 3184, 3369
Sfr303I CCGCGG 1 cut(s) 688
SfuI TTCGAA 1 cut(s) 1458
SgrBI CCGCGG 1 cut(s) 688
SinI GGWCC 1 cut(s) 2858
SmlI CTYRAG 1 cut(s) 923
SmoI CTYRAG 1 cut(s) 923
SphI GCATGC 1 cut(s) 2116
SspI AATATT 1 cut(s) 1015
SspMI CTAG 5 cut(s) 48, 509, 737, 1742, 2342
SstI GAGCTC 1 cut(s) 1805
StyD4I CCNGG 5 cut(s) 200, 1728, 2279, 2713, 2860
StyI CCWWGG 4 cut(s) 1504, 2326, 2494, 2910
TaaI ACNGT 9 cut(s) 57, 228, 1790, 1843, 1858, 2009, 2723, 3074, 3196
TatI WGTACW 4 cut(s) 74, 798, 2698, 3132
TauI GCSGC 1 cut(s) 2540
TfiI GAWTC 8 cut(s) 761, 967, 1658, 2739, 2767, 2847, 2871, 3077
TscAI CASTG 8 cut(s) 151, 220, 1954, 2678, 2737, 2911, 3193, 3394
TseFI GTSAC 7 cut(s) 152, 1588, 2003, 2099, 2675, 2914, 3188
TseI GCWGC 7 cut(s) 724, 910, 1335, 1338, 1762, 1765, 2234
Tsp45I GTSAC 7 cut(s) 152, 1588, 2003, 2099, 2675, 2914, 3188
TspGWI ACGGA 3 cut(s) 52, 765, 3002
TspRI CASTG 8 cut(s) 151, 220, 1954, 2678, 2737, 2911, 3193, 3394
Tth111I GACNNNGTC 1 cut(s) 2093
Van91I CCANNNNNTGG 1 cut(s) 2867
Vha464I CTTAAG 1 cut(s) 923
VneI GTGCAC 1 cut(s) 3383
VpaK11BI GGWCC 1 cut(s) 2858
XagI CCTNNNNNAGG 2 cut(s) 1420, 2571
XceI RCATGY 2 cut(s) 271, 2116
XmiI GTMKAC 3 cut(s) 162, 246, 1122
XmnI GAANNNNTTC 2 cut(s) 846, 2165
XspI CTAG 5 cut(s) 48, 509, 737, 1742, 2342
ZraI GACGTC 1 cut(s) 3226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.